AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1359010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1359010 MtrunA17_Chr1g0168671 98.611 72 1 0 1 72 78 149 1.14e-45 142
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1359010 AT1G04555.1 75.342 73 18 0 1 73 76 148 2.36e-31 107

Find 41 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTCTTCTGGGCTGTTTC+TGG 0.133948 tig0016633:-1336 None:intergenic
TCTGCTTCGTGTCTTTCTTC+TGG 0.172972 tig0016633:-1347 None:intergenic
TGCTAAGTTAAATGTGTAAT+AGG 0.269239 tig0016633:+3150 Msa1359010:three_prime_UTR
CTGCGGTTGATTTGTCTAAA+AGG 0.270731 tig0016633:+1373 Msa1359010:CDS
CTTTGGAGGAATGGCGTAAA+AGG 0.283674 tig0016633:+3028 Msa1359010:CDS
CTGCTTCGTGTCTTTCTTCT+GGG 0.289507 tig0016633:-1346 None:intergenic
GAAGGATGTCATTCCGGATA+TGG 0.315459 tig0016633:-3334 None:intergenic
CTTCTGGGCTGTTTCTGGTT+CGG 0.381648 tig0016633:-1331 None:intergenic
GCTAAGTTAAATGTGTAATA+GGG 0.401034 tig0016633:+3151 Msa1359010:three_prime_UTR
CAAGCTGGCGGCATCGTTCA+AGG 0.426188 tig0016633:-1412 None:intergenic
GCAAACAAGATCAAGGCATA+TGG 0.478442 tig0016633:-3437 None:intergenic
TGATTTGTCTAAAAGGCTAA+AGG 0.478881 tig0016633:+1380 Msa1359010:CDS
TATATAAAGTCAAGATTGAG+AGG 0.480669 tig0016633:-3100 None:intergenic
AACGATGCCGCCAGCTTGAA+AGG 0.485394 tig0016633:+1417 Msa1359010:CDS
GAGGGCGAGACAAAGACAAT+TGG 0.490395 tig0016633:+3059 Msa1359010:CDS
GATTCTCAGCTTTGGAGGAA+TGG 0.496368 tig0016633:+3019 Msa1359010:intron
AGAAAAGAGGAGAGACAGAG+AGG 0.501813 tig0016633:+3295 Msa1359010:three_prime_UTR
AAGAAGGAAGGATGTCATTC+CGG 0.507574 tig0016633:-3340 None:intergenic
CTTATAAGCAAACAAGATCA+AGG 0.509621 tig0016633:-3444 None:intergenic
TTCGTGATTCTCAGCTTTGG+AGG 0.517039 tig0016633:+3014 Msa1359010:intron
AGTGGAGAGAGAATAGAGAG+AGG 0.523701 tig0016633:+3257 Msa1359010:three_prime_UTR
AGACGGAGAGGATCCATATC+CGG 0.539155 tig0016633:+3321 Msa1359010:three_prime_UTR
TGAGAGAGATTGAGCAGGAA+GGG 0.541006 tig0016633:+1304 Msa1359010:CDS
GAGTTTACAAGAGAGAAAAG+AGG 0.555516 tig0016633:+3282 Msa1359010:three_prime_UTR
TCAGGTGAGAGAGATTGAGC+AGG 0.557629 tig0016633:+1299 None:intergenic
GAGAGACAGAGAGGATGAGA+CGG 0.566240 tig0016633:+3304 Msa1359010:three_prime_UTR
TGTGTAATAGGGAAATTAGC+AGG 0.567315 tig0016633:+3162 Msa1359010:three_prime_UTR
TGTTGAAAACAAAGAGAGAG+TGG 0.572542 tig0016633:+3239 Msa1359010:three_prime_UTR
AAGAGGAAAACCTTTCAAGC+TGG 0.581024 tig0016633:-1427 None:intergenic
GTGAGAGAGATTGAGCAGGA+AGG 0.582348 tig0016633:+1303 Msa1359010:CDS
TGGCGGCATCGTTCAAGGAA+CGG 0.593127 tig0016633:-1407 None:intergenic
GGTGAAGGTGAGTCACAAGA+AGG 0.598576 tig0016633:-3356 None:intergenic
AAAACAAAACGAGAAGGTGA+AGG 0.616029 tig0016633:-3371 None:intergenic
AAGGTGAGTCACAAGAAGGA+AGG 0.616323 tig0016633:-3352 None:intergenic
GCATCATCATCGTAACACAT+TGG 0.623456 tig0016633:+3209 Msa1359010:three_prime_UTR
AGGAAAACCTTTCAAGCTGG+CGG 0.625675 tig0016633:-1424 None:intergenic
GGCGTAAAAGGAAAATCGAG+AGG 0.668939 tig0016633:+3040 Msa1359010:CDS
ACAGAGAGGATGAGACGGAG+AGG 0.670450 tig0016633:+3309 Msa1359010:three_prime_UTR
ATAAAGTCAAGATTGAGAGG+AGG 0.675762 tig0016633:-3097 None:intergenic
GCGTAAAAGGAAAATCGAGA+GGG 0.690961 tig0016633:+3041 Msa1359010:CDS
AGACACGAAGCAGACAGCTG+CGG 0.704640 tig0016633:+1356 Msa1359010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTATTTTTAGGAAATA+GGG - tig0016633:1526-1545 None:intergenic 10.0%
!!! TAATTTTATTTTTAGGAAAT+AGG - tig0016633:1527-1546 None:intergenic 10.0%
!!! AAATTTACAATTTTGATATT+TGG + tig0016633:2627-2646 Msa1359010:intron 10.0%
!!! TCTTTATATGTATAATTTTT+AGG - tig0016633:3405-3424 None:intergenic 10.0%
!!! AATTTTATTTTTAGGAAATA+GGG - tig0016633:1526-1545 None:intergenic 10.0%
!!! TAATTTTATTTTTAGGAAAT+AGG - tig0016633:1527-1546 None:intergenic 10.0%
!!! AAATTTACAATTTTGATATT+TGG + tig0016633:2627-2646 Msa1359010:intron 10.0%
!!! TCTTTATATGTATAATTTTT+AGG - tig0016633:3405-3424 None:intergenic 10.0%
!! TCCTAAAAATAAAATTATTC+AGG + tig0016633:1530-1549 Msa1359010:intron 15.0%
!!! TCCTGAATAATTTTATTTTT+AGG - tig0016633:1534-1553 None:intergenic 15.0%
!! AAATAATTACTAAAACTCAT+TGG - tig0016633:1564-1583 None:intergenic 15.0%
!! TATTAACAATGATAAATTGA+AGG + tig0016633:1784-1803 Msa1359010:intron 15.0%
!! ATTAACAATGATAAATTGAA+GGG + tig0016633:1785-1804 Msa1359010:intron 15.0%
!! AAATGTCAATTAAATCATAT+AGG + tig0016633:1995-2014 Msa1359010:intron 15.0%
!!! GGTTTTTTAAAATGATAAAA+TGG + tig0016633:2408-2427 Msa1359010:intron 15.0%
!! AGTCGTTATATTATAAAAAT+AGG - tig0016633:2460-2479 None:intergenic 15.0%
!!! ATGAATTTTAAATATTTAGC+TGG - tig0016633:2600-2619 None:intergenic 15.0%
!!! GCTAAATATTTAAAATTCAT+TGG + tig0016633:2600-2619 Msa1359010:intron 15.0%
!! TCCTAAAAATAAAATTATTC+AGG + tig0016633:1530-1549 Msa1359010:intron 15.0%
!!! TCCTGAATAATTTTATTTTT+AGG - tig0016633:1534-1553 None:intergenic 15.0%
!! AAATAATTACTAAAACTCAT+TGG - tig0016633:1564-1583 None:intergenic 15.0%
!! TATTAACAATGATAAATTGA+AGG + tig0016633:1784-1803 Msa1359010:intron 15.0%
!! ATTAACAATGATAAATTGAA+GGG + tig0016633:1785-1804 Msa1359010:intron 15.0%
!! AAATGTCAATTAAATCATAT+AGG + tig0016633:1995-2014 Msa1359010:intron 15.0%
!!! GGTTTTTTAAAATGATAAAA+TGG + tig0016633:2408-2427 Msa1359010:intron 15.0%
!! AGTCGTTATATTATAAAAAT+AGG - tig0016633:2460-2479 None:intergenic 15.0%
!!! ATGAATTTTAAATATTTAGC+TGG - tig0016633:2600-2619 None:intergenic 15.0%
!!! GCTAAATATTTAAAATTCAT+TGG + tig0016633:2600-2619 Msa1359010:intron 15.0%
!! AAAATGGTTATGATTAAGAA+AGG - tig0016633:1466-1485 None:intergenic 20.0%
!!! TAATTAGTGTTGTTTGTAAT+TGG + tig0016633:1664-1683 Msa1359010:intron 20.0%
!!! AATTAGTGTTGTTTGTAATT+GGG + tig0016633:1665-1684 Msa1359010:intron 20.0%
!!! ATTGTTACTTTTTTGTTGAA+GGG - tig0016633:1743-1762 None:intergenic 20.0%
!!! TATTGTTACTTTTTTGTTGA+AGG - tig0016633:1744-1763 None:intergenic 20.0%
!! ATTGAACATTAGAAAATTGA+CGG + tig0016633:1836-1855 Msa1359010:intron 20.0%
!!! AATTGATTTGTGCATTTTTT+AGG + tig0016633:1947-1966 Msa1359010:intron 20.0%
!!! ATTTTTTAGGAGTGATATAA+TGG + tig0016633:1960-1979 Msa1359010:intron 20.0%
!! TCAATAAATGCTCAATTATA+TGG + tig0016633:2158-2177 Msa1359010:intron 20.0%
!! CAATAAATGCTCAATTATAT+GGG + tig0016633:2159-2178 Msa1359010:intron 20.0%
!! TTATAAAAATAGGTCATGTT+TGG - tig0016633:2450-2469 None:intergenic 20.0%
!!! ATTTATATTAGAAGCATGAA+GGG - tig0016633:2692-2711 None:intergenic 20.0%
!! AATAAATTCATGTAAGTTCA+AGG - tig0016633:2727-2746 None:intergenic 20.0%
!! AATCGTTTAATCCAAATATT+TGG - tig0016633:2834-2853 None:intergenic 20.0%
!! ATTAAACGATTCATGATATT+TGG + tig0016633:2843-2862 Msa1359010:intron 20.0%
!! AAAATGGTTATGATTAAGAA+AGG - tig0016633:1466-1485 None:intergenic 20.0%
!!! TAATTAGTGTTGTTTGTAAT+TGG + tig0016633:1664-1683 Msa1359010:intron 20.0%
!!! AATTAGTGTTGTTTGTAATT+GGG + tig0016633:1665-1684 Msa1359010:intron 20.0%
!!! ATTGTTACTTTTTTGTTGAA+GGG - tig0016633:1743-1762 None:intergenic 20.0%
!!! TATTGTTACTTTTTTGTTGA+AGG - tig0016633:1744-1763 None:intergenic 20.0%
!! ATTGAACATTAGAAAATTGA+CGG + tig0016633:1836-1855 Msa1359010:intron 20.0%
!!! AATTGATTTGTGCATTTTTT+AGG + tig0016633:1947-1966 Msa1359010:intron 20.0%
!!! ATTTTTTAGGAGTGATATAA+TGG + tig0016633:1960-1979 Msa1359010:intron 20.0%
!! TCAATAAATGCTCAATTATA+TGG + tig0016633:2158-2177 Msa1359010:intron 20.0%
!! CAATAAATGCTCAATTATAT+GGG + tig0016633:2159-2178 Msa1359010:intron 20.0%
!! TTATAAAAATAGGTCATGTT+TGG - tig0016633:2450-2469 None:intergenic 20.0%
!!! ATTTATATTAGAAGCATGAA+GGG - tig0016633:2692-2711 None:intergenic 20.0%
!! AATAAATTCATGTAAGTTCA+AGG - tig0016633:2727-2746 None:intergenic 20.0%
!! AATCGTTTAATCCAAATATT+TGG - tig0016633:2834-2853 None:intergenic 20.0%
!! ATTAAACGATTCATGATATT+TGG + tig0016633:2843-2862 Msa1359010:intron 20.0%
!! ATGATTAAGAAAGGTATGTA+GGG - tig0016633:1457-1476 None:intergenic 25.0%
!! TATGATTAAGAAAGGTATGT+AGG - tig0016633:1458-1477 None:intergenic 25.0%
! CTGTGTTTGCAAATAAAAAA+TGG - tig0016633:1482-1501 None:intergenic 25.0%
! TAGTGTGATATAATAAACTG+TGG + tig0016633:1603-1622 Msa1359010:intron 25.0%
! ATTTGTGTTTGAATGCTTAT+AGG + tig0016633:1693-1712 Msa1359010:intron 25.0%
! TTGAACATTAGAAAATTGAC+GGG + tig0016633:1837-1856 Msa1359010:intron 25.0%
! TAGTGTAATATGAGCAAAAT+GGG - tig0016633:2189-2208 None:intergenic 25.0%
! ATAGTGTAATATGAGCAAAA+TGG - tig0016633:2190-2209 None:intergenic 25.0%
!! TAGTTGCAAAAGAGAATTTT+TGG + tig0016633:2386-2405 Msa1359010:intron 25.0%
!! AGTTGCAAAAGAGAATTTTT+GGG + tig0016633:2387-2406 Msa1359010:intron 25.0%
! TTTAGCTGGAAGAAATATAT+AGG - tig0016633:2586-2605 None:intergenic 25.0%
!! GATTTATATTAGAAGCATGA+AGG - tig0016633:2693-2712 None:intergenic 25.0%
!! TCTTTAGAAAGAAGAACAAA+TGG - tig0016633:2800-2819 None:intergenic 25.0%
! TTCTTTGTTCTTGTAGAATT+TGG + tig0016633:2903-2922 Msa1359010:intron 25.0%
! ATTAAAACCAATGTCAATAC+AGG - tig0016633:2982-3001 None:intergenic 25.0%
! TATATAAAGTCAAGATTGAG+AGG - tig0016633:3103-3122 None:intergenic 25.0%
! TGCTAAGTTAAATGTGTAAT+AGG + tig0016633:3150-3169 Msa1359010:three_prime_UTR 25.0%
! GCTAAGTTAAATGTGTAATA+GGG + tig0016633:3151-3170 Msa1359010:three_prime_UTR 25.0%
! AAAGAAAAAACAAAACGAGA+AGG - tig0016633:3380-3399 None:intergenic 25.0%
!!! AGCATATCATTGTTACTTTT+TGG + tig0016633:3465-3484 Msa1359010:three_prime_UTR 25.0%
!! ATGATTAAGAAAGGTATGTA+GGG - tig0016633:1457-1476 None:intergenic 25.0%
!! TATGATTAAGAAAGGTATGT+AGG - tig0016633:1458-1477 None:intergenic 25.0%
! CTGTGTTTGCAAATAAAAAA+TGG - tig0016633:1482-1501 None:intergenic 25.0%
! TAGTGTGATATAATAAACTG+TGG + tig0016633:1603-1622 Msa1359010:intron 25.0%
! ATTTGTGTTTGAATGCTTAT+AGG + tig0016633:1693-1712 Msa1359010:intron 25.0%
! TTGAACATTAGAAAATTGAC+GGG + tig0016633:1837-1856 Msa1359010:intron 25.0%
! TAGTGTAATATGAGCAAAAT+GGG - tig0016633:2189-2208 None:intergenic 25.0%
! ATAGTGTAATATGAGCAAAA+TGG - tig0016633:2190-2209 None:intergenic 25.0%
!! TAGTTGCAAAAGAGAATTTT+TGG + tig0016633:2386-2405 Msa1359010:intron 25.0%
!! AGTTGCAAAAGAGAATTTTT+GGG + tig0016633:2387-2406 Msa1359010:intron 25.0%
! TTTAGCTGGAAGAAATATAT+AGG - tig0016633:2586-2605 None:intergenic 25.0%
!! GATTTATATTAGAAGCATGA+AGG - tig0016633:2693-2712 None:intergenic 25.0%
!! TCTTTAGAAAGAAGAACAAA+TGG - tig0016633:2800-2819 None:intergenic 25.0%
! TTCTTTGTTCTTGTAGAATT+TGG + tig0016633:2903-2922 Msa1359010:intron 25.0%
! ATTAAAACCAATGTCAATAC+AGG - tig0016633:2982-3001 None:intergenic 25.0%
! TATATAAAGTCAAGATTGAG+AGG - tig0016633:3103-3122 None:intergenic 25.0%
! TGCTAAGTTAAATGTGTAAT+AGG + tig0016633:3150-3169 Msa1359010:three_prime_UTR 25.0%
! GCTAAGTTAAATGTGTAATA+GGG + tig0016633:3151-3170 Msa1359010:three_prime_UTR 25.0%
! AAAGAAAAAACAAAACGAGA+AGG - tig0016633:3380-3399 None:intergenic 25.0%
!!! AGCATATCATTGTTACTTTT+TGG + tig0016633:3465-3484 Msa1359010:three_prime_UTR 25.0%
! TGATTTGTCTAAAAGGCTAA+AGG + tig0016633:1380-1399 Msa1359010:CDS 30.0%
! AGTCCTTTCAATTTGGTTTA+TGG + tig0016633:1812-1831 Msa1359010:intron 30.0%
! GTCCTTTCAATTTGGTTTAT+GGG + tig0016633:1813-1832 Msa1359010:intron 30.0%
ATCCCATAAACCAAATTGAA+AGG - tig0016633:1818-1837 None:intergenic 30.0%
TGAACATTAGAAAATTGACG+GGG + tig0016633:1838-1857 Msa1359010:intron 30.0%
ATATTGGCCAAAATATTAGG+GGG - tig0016633:2070-2089 None:intergenic 30.0%
GATATTGGCCAAAATATTAG+GGG - tig0016633:2071-2090 None:intergenic 30.0%
GGATATTGGCCAAAATATTA+GGG - tig0016633:2072-2091 None:intergenic 30.0%
CATTATTTGACAACAGTAGT+AGG - tig0016633:2248-2267 None:intergenic 30.0%
CTATCCTAGTTTCATTACAA+GGG + tig0016633:2325-2344 Msa1359010:intron 30.0%
TAATATAACGACTGAAGACA+CGG + tig0016633:2467-2486 Msa1359010:intron 30.0%
CAAAACAAATGAAAAGATGC+TGG + tig0016633:2520-2539 Msa1359010:intron 30.0%
! TGTTCTTCTTTCTAAAGACT+TGG + tig0016633:2802-2821 Msa1359010:intron 30.0%
!!! TGTTCTTTTTTCTGAAGACT+TGG - tig0016633:2875-2894 None:intergenic 30.0%
CAAAGACAATTGGAGAAAAA+TGG + tig0016633:3069-3088 Msa1359010:CDS 30.0%
CTTATAAGCAAACAAGATCA+AGG - tig0016633:3447-3466 None:intergenic 30.0%
! TATACTACAACATTTTGCTC+AGG + tig0016633:3517-3536 Msa1359010:three_prime_UTR 30.0%
! ATACTACAACATTTTGCTCA+GGG + tig0016633:3518-3537 Msa1359010:three_prime_UTR 30.0%
! TGATTTGTCTAAAAGGCTAA+AGG + tig0016633:1380-1399 Msa1359010:CDS 30.0%
! AGTCCTTTCAATTTGGTTTA+TGG + tig0016633:1812-1831 Msa1359010:intron 30.0%
! GTCCTTTCAATTTGGTTTAT+GGG + tig0016633:1813-1832 Msa1359010:intron 30.0%
ATCCCATAAACCAAATTGAA+AGG - tig0016633:1818-1837 None:intergenic 30.0%
TGAACATTAGAAAATTGACG+GGG + tig0016633:1838-1857 Msa1359010:intron 30.0%
ATATTGGCCAAAATATTAGG+GGG - tig0016633:2070-2089 None:intergenic 30.0%
GATATTGGCCAAAATATTAG+GGG - tig0016633:2071-2090 None:intergenic 30.0%
GGATATTGGCCAAAATATTA+GGG - tig0016633:2072-2091 None:intergenic 30.0%
CATTATTTGACAACAGTAGT+AGG - tig0016633:2248-2267 None:intergenic 30.0%
CTATCCTAGTTTCATTACAA+GGG + tig0016633:2325-2344 Msa1359010:intron 30.0%
TAATATAACGACTGAAGACA+CGG + tig0016633:2467-2486 Msa1359010:intron 30.0%
CAAAACAAATGAAAAGATGC+TGG + tig0016633:2520-2539 Msa1359010:intron 30.0%
! TGTTCTTCTTTCTAAAGACT+TGG + tig0016633:2802-2821 Msa1359010:intron 30.0%
!!! TGTTCTTTTTTCTGAAGACT+TGG - tig0016633:2875-2894 None:intergenic 30.0%
CAAAGACAATTGGAGAAAAA+TGG + tig0016633:3069-3088 Msa1359010:CDS 30.0%
CTTATAAGCAAACAAGATCA+AGG - tig0016633:3447-3466 None:intergenic 30.0%
! TATACTACAACATTTTGCTC+AGG + tig0016633:3517-3536 Msa1359010:three_prime_UTR 30.0%
! ATACTACAACATTTTGCTCA+GGG + tig0016633:3518-3537 Msa1359010:three_prime_UTR 30.0%
AAGGTATGTAGGGAAAAAAG+AGG - tig0016633:1447-1466 None:intergenic 35.0%
CAACACTAATTAGAGATCCT+CGG - tig0016633:1656-1675 None:intergenic 35.0%
TATTGGCCAAAATATTAGGG+GGG - tig0016633:2069-2088 None:intergenic 35.0%
CGGATATTGGCCAAAATATT+AGG - tig0016633:2073-2092 None:intergenic 35.0%
AATACTAGTCCTATAAGCAG+TGG - tig0016633:2096-2115 None:intergenic 35.0%
TGCTCATATTACACTATGTC+TGG + tig0016633:2193-2212 Msa1359010:intron 35.0%
!! TAGGCTTTAAATCGAGGAAA+AGG - tig0016633:2229-2248 None:intergenic 35.0%
GTTGTCAAATAATGCGATAG+CGG + tig0016633:2254-2273 Msa1359010:intron 35.0%
GCTATCCTAGTTTCATTACA+AGG + tig0016633:2324-2343 Msa1359010:intron 35.0%
ATTGCCCTTGTAATGAAACT+AGG - tig0016633:2332-2351 None:intergenic 35.0%
AGATGCTGGCATTAATCATT+CGG + tig0016633:2534-2553 Msa1359010:intron 35.0%
CTTGGCTTATGCCAAATATT+TGG + tig0016633:2820-2839 Msa1359010:intron 35.0%
! TCTGAATCCTGTATTGACAT+TGG + tig0016633:2972-2991 Msa1359010:intron 35.0%
ATAAAGTCAAGATTGAGAGG+AGG - tig0016633:3100-3119 None:intergenic 35.0%
TGTGTAATAGGGAAATTAGC+AGG + tig0016633:3162-3181 Msa1359010:three_prime_UTR 35.0%
TGTTGAAAACAAAGAGAGAG+TGG + tig0016633:3239-3258 Msa1359010:three_prime_UTR 35.0%
GAGTTTACAAGAGAGAAAAG+AGG + tig0016633:3282-3301 Msa1359010:three_prime_UTR 35.0%
AAAACAAAACGAGAAGGTGA+AGG - tig0016633:3374-3393 None:intergenic 35.0%
AAGGTATGTAGGGAAAAAAG+AGG - tig0016633:1447-1466 None:intergenic 35.0%
CAACACTAATTAGAGATCCT+CGG - tig0016633:1656-1675 None:intergenic 35.0%
TATTGGCCAAAATATTAGGG+GGG - tig0016633:2069-2088 None:intergenic 35.0%
CGGATATTGGCCAAAATATT+AGG - tig0016633:2073-2092 None:intergenic 35.0%
AATACTAGTCCTATAAGCAG+TGG - tig0016633:2096-2115 None:intergenic 35.0%
TGCTCATATTACACTATGTC+TGG + tig0016633:2193-2212 Msa1359010:intron 35.0%
!! TAGGCTTTAAATCGAGGAAA+AGG - tig0016633:2229-2248 None:intergenic 35.0%
GTTGTCAAATAATGCGATAG+CGG + tig0016633:2254-2273 Msa1359010:intron 35.0%
GCTATCCTAGTTTCATTACA+AGG + tig0016633:2324-2343 Msa1359010:intron 35.0%
ATTGCCCTTGTAATGAAACT+AGG - tig0016633:2332-2351 None:intergenic 35.0%
AGATGCTGGCATTAATCATT+CGG + tig0016633:2534-2553 Msa1359010:intron 35.0%
CTTGGCTTATGCCAAATATT+TGG + tig0016633:2820-2839 Msa1359010:intron 35.0%
! TCTGAATCCTGTATTGACAT+TGG + tig0016633:2972-2991 Msa1359010:intron 35.0%
ATAAAGTCAAGATTGAGAGG+AGG - tig0016633:3100-3119 None:intergenic 35.0%
TGTGTAATAGGGAAATTAGC+AGG + tig0016633:3162-3181 Msa1359010:three_prime_UTR 35.0%
TGTTGAAAACAAAGAGAGAG+TGG + tig0016633:3239-3258 Msa1359010:three_prime_UTR 35.0%
GAGTTTACAAGAGAGAAAAG+AGG + tig0016633:3282-3301 Msa1359010:three_prime_UTR 35.0%
AAAACAAAACGAGAAGGTGA+AGG - tig0016633:3374-3393 None:intergenic 35.0%
!! CTGCGGTTGATTTGTCTAAA+AGG + tig0016633:1373-1392 Msa1359010:CDS 40.0%
AAGAGGAAAACCTTTCAAGC+TGG - tig0016633:1430-1449 None:intergenic 40.0%
GTTTGAATGCTTATAGGCAG+TGG + tig0016633:1699-1718 Msa1359010:intron 40.0%
GGGTGTTAGTCCTTTCAATT+TGG + tig0016633:1805-1824 Msa1359010:intron 40.0%
CATTAGAAAATTGACGGGGT+TGG + tig0016633:1842-1861 Msa1359010:intron 40.0%
! TTACACTATGTCTGGAACCT+AGG + tig0016633:2201-2220 Msa1359010:intron 40.0%
AAATCGAGGAAAAGGATCCT+AGG - tig0016633:2221-2240 None:intergenic 40.0%
!! CAGTAGTAGGCTTTAAATCG+AGG - tig0016633:2235-2254 None:intergenic 40.0%
TGGCATTAATCATTCGGCAA+TGG + tig0016633:2540-2559 Msa1359010:intron 40.0%
! GAAGGGCATAAAAAGATTGC+AGG - tig0016633:2675-2694 None:intergenic 40.0%
CTGAGAATCACGAACATCAA+AGG - tig0016633:3008-3027 None:intergenic 40.0%
ATGTTCGTGATTCTCAGCTT+TGG + tig0016633:3011-3030 Msa1359010:intron 40.0%
GCGTAAAAGGAAAATCGAGA+GGG + tig0016633:3041-3060 Msa1359010:CDS 40.0%
GCATCATCATCGTAACACAT+TGG + tig0016633:3209-3228 Msa1359010:three_prime_UTR 40.0%
AAGAAGGAAGGATGTCATTC+CGG - tig0016633:3343-3362 None:intergenic 40.0%
GCAAACAAGATCAAGGCATA+TGG - tig0016633:3440-3459 None:intergenic 40.0%
!! CTGCGGTTGATTTGTCTAAA+AGG + tig0016633:1373-1392 Msa1359010:CDS 40.0%
AAGAGGAAAACCTTTCAAGC+TGG - tig0016633:1430-1449 None:intergenic 40.0%
GTTTGAATGCTTATAGGCAG+TGG + tig0016633:1699-1718 Msa1359010:intron 40.0%
GGGTGTTAGTCCTTTCAATT+TGG + tig0016633:1805-1824 Msa1359010:intron 40.0%
CATTAGAAAATTGACGGGGT+TGG + tig0016633:1842-1861 Msa1359010:intron 40.0%
! TTACACTATGTCTGGAACCT+AGG + tig0016633:2201-2220 Msa1359010:intron 40.0%
AAATCGAGGAAAAGGATCCT+AGG - tig0016633:2221-2240 None:intergenic 40.0%
!! CAGTAGTAGGCTTTAAATCG+AGG - tig0016633:2235-2254 None:intergenic 40.0%
TGGCATTAATCATTCGGCAA+TGG + tig0016633:2540-2559 Msa1359010:intron 40.0%
! GAAGGGCATAAAAAGATTGC+AGG - tig0016633:2675-2694 None:intergenic 40.0%
CTGAGAATCACGAACATCAA+AGG - tig0016633:3008-3027 None:intergenic 40.0%
ATGTTCGTGATTCTCAGCTT+TGG + tig0016633:3011-3030 Msa1359010:intron 40.0%
GCGTAAAAGGAAAATCGAGA+GGG + tig0016633:3041-3060 Msa1359010:CDS 40.0%
GCATCATCATCGTAACACAT+TGG + tig0016633:3209-3228 Msa1359010:three_prime_UTR 40.0%
AAGAAGGAAGGATGTCATTC+CGG - tig0016633:3343-3362 None:intergenic 40.0%
GCAAACAAGATCAAGGCATA+TGG - tig0016633:3440-3459 None:intergenic 40.0%
TGAGAGAGATTGAGCAGGAA+GGG + tig0016633:1304-1323 Msa1359010:CDS 45.0%
! TCTTTCTTCTGGGCTGTTTC+TGG - tig0016633:1339-1358 None:intergenic 45.0%
CTGCTTCGTGTCTTTCTTCT+GGG - tig0016633:1349-1368 None:intergenic 45.0%
TCTGCTTCGTGTCTTTCTTC+TGG - tig0016633:1350-1369 None:intergenic 45.0%
AGGAAAACCTTTCAAGCTGG+CGG - tig0016633:1427-1446 None:intergenic 45.0%
TTAGAGATCCTCGGCTGATA+TGG - tig0016633:1647-1666 None:intergenic 45.0%
AATTGACGGGGTTGGATGTT+TGG + tig0016633:1850-1869 Msa1359010:intron 45.0%
!! CTATAAGCAGTGGCGGATAT+TGG - tig0016633:2086-2105 None:intergenic 45.0%
CAATATCCGCCACTGCTTAT+AGG + tig0016633:2084-2103 Msa1359010:intron 45.0%
ACTAGTCCTATAAGCAGTGG+CGG - tig0016633:2093-2112 None:intergenic 45.0%
ATGAAACTAGGATAGCAGCG+AGG - tig0016633:2320-2339 None:intergenic 45.0%
TAGCAGAAACATGTAGTGGC+GGG - tig0016633:2359-2378 None:intergenic 45.0%
CTAGCAGAAACATGTAGTGG+CGG - tig0016633:2360-2379 None:intergenic 45.0%
CACCTAGCAGAAACATGTAG+TGG - tig0016633:2363-2382 None:intergenic 45.0%
AAATATATAGGACCCCCAGC+TGG - tig0016633:2574-2593 None:intergenic 45.0%
! TTCGTGATTCTCAGCTTTGG+AGG + tig0016633:3014-3033 Msa1359010:intron 45.0%
!! GATTCTCAGCTTTGGAGGAA+TGG + tig0016633:3019-3038 Msa1359010:intron 45.0%
! CTTTGGAGGAATGGCGTAAA+AGG + tig0016633:3028-3047 Msa1359010:CDS 45.0%
GGCGTAAAAGGAAAATCGAG+AGG + tig0016633:3040-3059 Msa1359010:CDS 45.0%
AGTGGAGAGAGAATAGAGAG+AGG + tig0016633:3257-3276 Msa1359010:three_prime_UTR 45.0%
AGAAAAGAGGAGAGACAGAG+AGG + tig0016633:3295-3314 Msa1359010:three_prime_UTR 45.0%
GAAGGATGTCATTCCGGATA+TGG - tig0016633:3337-3356 None:intergenic 45.0%
AAGGTGAGTCACAAGAAGGA+AGG - tig0016633:3355-3374 None:intergenic 45.0%
TGAGAGAGATTGAGCAGGAA+GGG + tig0016633:1304-1323 Msa1359010:CDS 45.0%
! TCTTTCTTCTGGGCTGTTTC+TGG - tig0016633:1339-1358 None:intergenic 45.0%
CTGCTTCGTGTCTTTCTTCT+GGG - tig0016633:1349-1368 None:intergenic 45.0%
TCTGCTTCGTGTCTTTCTTC+TGG - tig0016633:1350-1369 None:intergenic 45.0%
AGGAAAACCTTTCAAGCTGG+CGG - tig0016633:1427-1446 None:intergenic 45.0%
TTAGAGATCCTCGGCTGATA+TGG - tig0016633:1647-1666 None:intergenic 45.0%
AATTGACGGGGTTGGATGTT+TGG + tig0016633:1850-1869 Msa1359010:intron 45.0%
!! CTATAAGCAGTGGCGGATAT+TGG - tig0016633:2086-2105 None:intergenic 45.0%
CAATATCCGCCACTGCTTAT+AGG + tig0016633:2084-2103 Msa1359010:intron 45.0%
ACTAGTCCTATAAGCAGTGG+CGG - tig0016633:2093-2112 None:intergenic 45.0%
ATGAAACTAGGATAGCAGCG+AGG - tig0016633:2320-2339 None:intergenic 45.0%
TAGCAGAAACATGTAGTGGC+GGG - tig0016633:2359-2378 None:intergenic 45.0%
CTAGCAGAAACATGTAGTGG+CGG - tig0016633:2360-2379 None:intergenic 45.0%
CACCTAGCAGAAACATGTAG+TGG - tig0016633:2363-2382 None:intergenic 45.0%
AAATATATAGGACCCCCAGC+TGG - tig0016633:2574-2593 None:intergenic 45.0%
! TTCGTGATTCTCAGCTTTGG+AGG + tig0016633:3014-3033 Msa1359010:intron 45.0%
!! GATTCTCAGCTTTGGAGGAA+TGG + tig0016633:3019-3038 Msa1359010:intron 45.0%
! CTTTGGAGGAATGGCGTAAA+AGG + tig0016633:3028-3047 Msa1359010:CDS 45.0%
GGCGTAAAAGGAAAATCGAG+AGG + tig0016633:3040-3059 Msa1359010:CDS 45.0%
AGTGGAGAGAGAATAGAGAG+AGG + tig0016633:3257-3276 Msa1359010:three_prime_UTR 45.0%
AGAAAAGAGGAGAGACAGAG+AGG + tig0016633:3295-3314 Msa1359010:three_prime_UTR 45.0%
GAAGGATGTCATTCCGGATA+TGG - tig0016633:3337-3356 None:intergenic 45.0%
AAGGTGAGTCACAAGAAGGA+AGG - tig0016633:3355-3374 None:intergenic 45.0%
GTGAGAGAGATTGAGCAGGA+AGG + tig0016633:1303-1322 Msa1359010:CDS 50.0%
!! CTTCTGGGCTGTTTCTGGTT+CGG - tig0016633:1334-1353 None:intergenic 50.0%
TGGCCAAAATATTAGGGGGG+CGG - tig0016633:2066-2085 None:intergenic 50.0%
! CGCCACTACATGTTTCTGCT+AGG + tig0016633:2358-2377 Msa1359010:intron 50.0%
GAGGGCGAGACAAAGACAAT+TGG + tig0016633:3059-3078 Msa1359010:CDS 50.0%
GAGAGACAGAGAGGATGAGA+CGG + tig0016633:3304-3323 Msa1359010:three_prime_UTR 50.0%
AGACGGAGAGGATCCATATC+CGG + tig0016633:3321-3340 Msa1359010:three_prime_UTR 50.0%
GGTGAAGGTGAGTCACAAGA+AGG - tig0016633:3359-3378 None:intergenic 50.0%
GTGAGAGAGATTGAGCAGGA+AGG + tig0016633:1303-1322 Msa1359010:CDS 50.0%
!! CTTCTGGGCTGTTTCTGGTT+CGG - tig0016633:1334-1353 None:intergenic 50.0%
TGGCCAAAATATTAGGGGGG+CGG - tig0016633:2066-2085 None:intergenic 50.0%
! CGCCACTACATGTTTCTGCT+AGG + tig0016633:2358-2377 Msa1359010:intron 50.0%
GAGGGCGAGACAAAGACAAT+TGG + tig0016633:3059-3078 Msa1359010:CDS 50.0%
GAGAGACAGAGAGGATGAGA+CGG + tig0016633:3304-3323 Msa1359010:three_prime_UTR 50.0%
AGACGGAGAGGATCCATATC+CGG + tig0016633:3321-3340 Msa1359010:three_prime_UTR 50.0%
GGTGAAGGTGAGTCACAAGA+AGG - tig0016633:3359-3378 None:intergenic 50.0%
AGACACGAAGCAGACAGCTG+CGG + tig0016633:1356-1375 Msa1359010:CDS 55.0%
TGGCGGCATCGTTCAAGGAA+CGG - tig0016633:1410-1429 None:intergenic 55.0%
AACGATGCCGCCAGCTTGAA+AGG + tig0016633:1417-1436 Msa1359010:CDS 55.0%
AACCACAGCCATATCAGCCG+AGG + tig0016633:1636-1655 Msa1359010:intron 55.0%
ATCCTCGGCTGATATGGCTG+TGG - tig0016633:1641-1660 None:intergenic 55.0%
GCTTATAGGCAGTGGCTGAG+AGG + tig0016633:1707-1726 Msa1359010:intron 55.0%
! TGCCCGCCCCCCTAATATTT+TGG + tig0016633:2060-2079 Msa1359010:intron 55.0%
GGCCAAAATATTAGGGGGGC+GGG - tig0016633:2065-2084 None:intergenic 55.0%
CAATGGCGACTCAACCAGCT+GGG + tig0016633:2557-2576 Msa1359010:intron 55.0%
AATGGCGACTCAACCAGCTG+GGG + tig0016633:2558-2577 Msa1359010:intron 55.0%
ACAGAGAGGATGAGACGGAG+AGG + tig0016633:3309-3328 Msa1359010:three_prime_UTR 55.0%
AGACACGAAGCAGACAGCTG+CGG + tig0016633:1356-1375 Msa1359010:CDS 55.0%
TGGCGGCATCGTTCAAGGAA+CGG - tig0016633:1410-1429 None:intergenic 55.0%
AACGATGCCGCCAGCTTGAA+AGG + tig0016633:1417-1436 Msa1359010:CDS 55.0%
AACCACAGCCATATCAGCCG+AGG + tig0016633:1636-1655 Msa1359010:intron 55.0%
ATCCTCGGCTGATATGGCTG+TGG - tig0016633:1641-1660 None:intergenic 55.0%
GCTTATAGGCAGTGGCTGAG+AGG + tig0016633:1707-1726 Msa1359010:intron 55.0%
! TGCCCGCCCCCCTAATATTT+TGG + tig0016633:2060-2079 Msa1359010:intron 55.0%
GGCCAAAATATTAGGGGGGC+GGG - tig0016633:2065-2084 None:intergenic 55.0%
CAATGGCGACTCAACCAGCT+GGG + tig0016633:2557-2576 Msa1359010:intron 55.0%
AATGGCGACTCAACCAGCTG+GGG + tig0016633:2558-2577 Msa1359010:intron 55.0%
ACAGAGAGGATGAGACGGAG+AGG + tig0016633:3309-3328 Msa1359010:three_prime_UTR 55.0%
CAAGCTGGCGGCATCGTTCA+AGG - tig0016633:1415-1434 None:intergenic 60.0%
GCAATGGCGACTCAACCAGC+TGG + tig0016633:2556-2575 Msa1359010:intron 60.0%
ATGGCGACTCAACCAGCTGG+GGG + tig0016633:2559-2578 Msa1359010:intron 60.0%
CAAGCTGGCGGCATCGTTCA+AGG - tig0016633:1415-1434 None:intergenic 60.0%
GCAATGGCGACTCAACCAGC+TGG + tig0016633:2556-2575 Msa1359010:intron 60.0%
ATGGCGACTCAACCAGCTGG+GGG + tig0016633:2559-2578 Msa1359010:intron 60.0%
Chromosome Type Strat End Strand Name
tig0016633 gene 1303 3555 1303 ID=Msa1359010;Name=Msa1359010
tig0016633 mRNA 1303 3555 1303 ID=Msa1359010-mRNA-1;Parent=Msa1359010;Name=Msa1359010-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|1|1|1|1|2|442|73
tig0016633 exon 1303 1438 1303 ID=Msa1359010-mRNA-1:exon:11156;Parent=Msa1359010-mRNA-1
tig0016633 exon 3028 3555 3028 ID=Msa1359010-mRNA-1:exon:11157;Parent=Msa1359010-mRNA-1
tig0016633 CDS 1303 1438 1303 ID=Msa1359010-mRNA-1:cds;Parent=Msa1359010-mRNA-1
tig0016633 CDS 3028 3113 3028 ID=Msa1359010-mRNA-1:cds;Parent=Msa1359010-mRNA-1
tig0016633 three_prime_UTR 3114 3555 3114 ID=Msa1359010-mRNA-1:three_prime_utr;Parent=Msa1359010-mRNA-1
Gene Sequence

>Msa1359010

GTGAGAGAGATTGAGCAGGAAGGGTACACCGAACCAGAAACAGCCCAGAAGAAAGACACGAAGCAGACAGCTGCGGTTGATTTGTCTAAAAGGCTAAAGGATTTCCGTTCCTTGAACGATGCCGCCAGCTTGAAAGCTTTGGAGGAATGGCGTAAAAGGAAAATCGAGAGGGCGAGACAAAGACAATTGGAGAAAAATGGAACAACCTCCTCTCAATCTTGA
Protein sequence

>Msa1359010

VREIEQEGYTEPETAQKKDTKQTAAVDLSKRLKDFRSLNDAASLKALEEWRKRKIERARQRQLEKNGTTSSQS*