AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1364010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364010 MtrunA17_Chr2g0330081 82.065 184 21 1 1 172 82 265 1.70e-93 275
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364010 AT3G13780.1 39.409 203 113 3 2 194 99 301 6.99e-28 107

Find 76 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACAAATTGATCCTGTTAA+AGG 0.155025 tig0018106:+53249 Msa1364010:CDS
GAAATTCGAAATGGGTCATT+TGG 0.296722 tig0018106:+53122 Msa1364010:exon
TCAAAGTTATTACCTTTAAC+AGG 0.314979 tig0018106:-53261 None:intergenic
AAGGTTTCATTTCAAGTCAT+TGG 0.319227 tig0018106:+53045 Msa1364010:five_prime_UTR
GAATGAAGCCTTGTATGTTT+AGG 0.320634 tig0018106:+55258 Msa1364010:three_prime_UTR
CTGTTTCTCGTCATCACTTT+CGG 0.323541 tig0018106:+52987 Msa1364010:five_prime_UTR
TCATTGGAAGAAACCCTATC+TGG 0.338307 tig0018106:+53061 Msa1364010:five_prime_UTR
TGATCCGGAATGTGGAGAAT+TGG 0.345241 tig0018106:+54692 Msa1364010:CDS
ATGTTCTCATAACTGATAAT+TGG 0.345555 tig0018106:+55337 Msa1364010:three_prime_UTR
TCATCCTCACTTTCTTTGCT+AGG 0.386031 tig0018106:-54733 None:intergenic
TTGTTCTTCAAAACCCAGAT+AGG 0.388052 tig0018106:-53075 None:intergenic
GGATCATGCCCTTTGGATAC+TGG 0.392754 tig0018106:-54675 None:intergenic
GTATCTTTGATATCTCTCTT+AGG 0.392852 tig0018106:-54922 None:intergenic
TGTCTGCGAAAACACCCTTT+TGG 0.394919 tig0018106:+53166 Msa1364010:CDS
AAGGAACTTGTTGCTAAGGT+TGG 0.407977 tig0018106:+54850 Msa1364010:CDS
CTTCTTCAAATTGAACCAAA+AGG 0.419549 tig0018106:-53181 None:intergenic
TGAGGATGAGGAAGAGGAAG+AGG 0.426013 tig0018106:+55056 Msa1364010:CDS
GATCCGGAATGTGGAGAATT+GGG 0.436535 tig0018106:+54693 Msa1364010:CDS
AGGAACTTGTTGCTAAGGTT+GGG 0.441377 tig0018106:+54851 Msa1364010:CDS
ACATTCCGGATCATGCCCTT+TGG 0.441791 tig0018106:-54682 None:intergenic
TGAGGAAGAGGAAGAGGAGG+AGG 0.442921 tig0018106:+55062 Msa1364010:CDS
TGAGGATAAAAGGAAAATGA+AGG 0.453909 tig0018106:+54765 Msa1364010:CDS
GAAATGGGTCATTTGGAACT+TGG 0.454601 tig0018106:+53129 Msa1364010:exon
ACACTGTTCGGACGAGGTTG+CGG 0.458112 tig0018106:+52944 Msa1364010:five_prime_UTR
ATGACTTGAAATGAAACCTT+AGG 0.465906 tig0018106:-53042 None:intergenic
TTCTTCAAATTGAACCAAAA+GGG 0.471521 tig0018106:-53180 None:intergenic
TGGTGATAGTGACGATGATA+AGG 0.485152 tig0018106:+54831 Msa1364010:CDS
TGACAATGAGGATGAGGAAG+AGG 0.499436 tig0018106:+55050 Msa1364010:CDS
CATCACAAGGCCACGGCACT+TGG 0.512507 tig0018106:-55159 None:intergenic
TAGCAAAGAAAGTGAGGATG+AGG 0.513147 tig0018106:+54735 Msa1364010:CDS
GCTGTGGCGGTGTAAAATCA+AGG 0.516324 tig0018106:+55111 Msa1364010:exon
AACAGCGACAAAACACTGTT+CGG 0.516498 tig0018106:+52932 Msa1364010:five_prime_UTR
AATCAGACTCGTTGTTGAGT+TGG 0.519462 tig0018106:-53011 None:intergenic
TGTTCTTCAAAACCCAGATA+GGG 0.524313 tig0018106:-53074 None:intergenic
ACTCCCAATTCTCCACATTC+CGG 0.528495 tig0018106:-54696 None:intergenic
TGATAAGGAACTTGTTGCTA+AGG 0.529588 tig0018106:+54846 Msa1364010:CDS
GCAAGGTCTAAAGACAATAG+AGG 0.531239 tig0018106:+54898 Msa1364010:CDS
AGTATCCAAAGGGCATGATC+CGG 0.532093 tig0018106:+54677 Msa1364010:CDS
ATGGAGTTCGAAGGCGAAGA+TGG 0.533843 tig0018106:+52890 Msa1364010:five_prime_UTR
GTCTGATTCAGAGCCTCCTA+AGG 0.535965 tig0018106:+53026 Msa1364010:five_prime_UTR
TGATGAAGAAGATGAGGATG+AGG 0.536649 tig0018106:+55017 Msa1364010:CDS
GGATGAGGAAGAGGAAGAGG+AGG 0.536791 tig0018106:+55059 Msa1364010:CDS
TTAGACCTTGCGGAGTACCT+AGG 0.545892 tig0018106:-54886 None:intergenic
CATTGGAAGAAACCCTATCT+GGG 0.552765 tig0018106:+53062 Msa1364010:five_prime_UTR
GACAAAACACTGTTCGGACG+AGG 0.556592 tig0018106:+52938 Msa1364010:five_prime_UTR
AAACAATGACAATGAGGATG+AGG 0.562409 tig0018106:+55044 Msa1364010:CDS
GAGGATAAAAGGAAAATGAA+GGG 0.567105 tig0018106:+54766 Msa1364010:CDS
TCTATTGTCTTTAGACCTTG+CGG 0.572441 tig0018106:-54896 None:intergenic
TTGGGAAAGGTAAGAAACCT+AGG 0.574841 tig0018106:+54869 Msa1364010:CDS
AACAAAGATGACGAGTGGAC+TGG 0.577011 tig0018106:+54811 Msa1364010:CDS
CTTGTTGCTAAGGTTGGGAA+AGG 0.577595 tig0018106:+54856 Msa1364010:CDS
GAGTTTGACCAGTATCCAAA+GGG 0.590357 tig0018106:+54667 Msa1364010:CDS
ACTTGAAATGAAACCTTAGG+AGG 0.593531 tig0018106:-53039 None:intergenic
CTGTGGCGGTGTAAAATCAA+GGG 0.601243 tig0018106:+55112 Msa1364010:exon
AAAGGGCATGATCCGGAATG+TGG 0.603271 tig0018106:+54684 Msa1364010:CDS
GACGATGATGAGACGCTAGG+AGG 0.603685 tig0018106:+54982 Msa1364010:CDS
CACTACATATCAACATCACA+AGG 0.606768 tig0018106:-55172 None:intergenic
AACATAGTCCTAAACATACA+AGG 0.620114 tig0018106:-55266 None:intergenic
TGAGTTTGACCAGTATCCAA+AGG 0.622122 tig0018106:+54666 Msa1364010:CDS
GGAGCCTAGCAAAGAAAGTG+AGG 0.635527 tig0018106:+54729 Msa1364010:CDS
GAGACAACAAAGATGACGAG+TGG 0.639451 tig0018106:+54806 Msa1364010:CDS
GAATTGGGAGTGGTTTCTCG+AGG 0.640949 tig0018106:+54708 Msa1364010:CDS
TAATTCTGAATGGAGTTCGA+AGG 0.641013 tig0018106:+52881 Msa1364010:five_prime_UTR
GGAATGTGGAGAATTGGGAG+TGG 0.641413 tig0018106:+54698 Msa1364010:CDS
GGAACAAAACAATGACAATG+AGG 0.654439 tig0018106:+55038 Msa1364010:CDS
ATATCAACATCACAAGGCCA+CGG 0.654785 tig0018106:-55166 None:intergenic
TGTTACTGATGAAGAAGATG+AGG 0.658205 tig0018106:+55011 Msa1364010:CDS
GATGACGATGATGAGACGCT+AGG 0.662670 tig0018106:+54979 Msa1364010:CDS
TGTTTAGATACCAAGTGCCG+TGG 0.663591 tig0018106:+55149 Msa1364010:three_prime_UTR
AGATATCAAAGATACTAGTG+TGG 0.683203 tig0018106:+54930 Msa1364010:CDS
AGAAACCTAGGTACTCCGCA+AGG 0.683889 tig0018106:+54881 Msa1364010:CDS
AGAAGATGACGATGATGCTG+TGG 0.684484 tig0018106:+55095 Msa1364010:CDS
TGTGGCGGTGTAAAATCAAG+GGG 0.688596 tig0018106:+55113 Msa1364010:exon
AGATGACGATGATGCTGTGG+CGG 0.690723 tig0018106:+55098 Msa1364010:CDS
AGTGATGACGAGAAACAGTG+TGG 0.729424 tig0018106:-52982 None:intergenic
GTGGCGGTGTAAAATCAAGG+GGG 0.792447 tig0018106:+55114 Msa1364010:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAATTCATTTTCAATATA+AGG + tig0018106:53992-54011 Msa1364010:intron 10.0%
!!! TTTTAACAAAATCGATAATT+TGG + tig0018106:53360-53379 Msa1364010:intron 15.0%
!! TAATTTATTTAAGTATATCC+AGG + tig0018106:53603-53622 Msa1364010:intron 15.0%
!!! AGTTTATTTTTGTGTTAATT+TGG + tig0018106:53931-53950 Msa1364010:intron 15.0%
!! TACAAATTCCAATATTATTT+AGG + tig0018106:54069-54088 Msa1364010:intron 15.0%
!! ATTATTCAGAAAAACATGTT+TGG - tig0018106:52865-52884 None:intergenic 20.0%
!!! TTTTTCTGAATAATTCTGAA+TGG + tig0018106:52871-52890 Msa1364010:five_prime_UTR 20.0%
!!! ATTTTCAAGAAATTCGAAAT+GGG + tig0018106:53114-53133 Msa1364010:exon 20.0%
!! AAAAATAGTTTCCATCAAAA+GGG - tig0018106:53288-53307 None:intergenic 20.0%
!! TAGAATTATACTATCCTTAA+AGG - tig0018106:53567-53586 None:intergenic 20.0%
!! TTAAGGATAGTATAATTCTA+AGG + tig0018106:53567-53586 Msa1364010:intron 20.0%
!! AATAAGTTAAAAACAACTCA+TGG - tig0018106:53705-53724 None:intergenic 20.0%
!! CTTATATATAAGCACTTATA+TGG + tig0018106:53816-53835 Msa1364010:intron 20.0%
!!! TTCATTTTCAATATAAGGTA+TGG + tig0018106:53997-54016 Msa1364010:intron 20.0%
!!! TCATTTTCAATATAAGGTAT+GGG + tig0018106:53998-54017 Msa1364010:intron 20.0%
!! AACTTGATCCTAAATAATAT+TGG - tig0018106:54080-54099 None:intergenic 20.0%
!!! GATTTTCAAGAAATTCGAAA+TGG + tig0018106:53113-53132 Msa1364010:exon 25.0%
! TTCTTCAAATTGAACCAAAA+GGG - tig0018106:53183-53202 None:intergenic 25.0%
! TCAAAGTTATTACCTTTAAC+AGG - tig0018106:53264-53283 None:intergenic 25.0%
! AAAATAGTTTCCATCAAAAG+GGG - tig0018106:53287-53306 None:intergenic 25.0%
! CAAAAATAGTTTCCATCAAA+AGG - tig0018106:53289-53308 None:intergenic 25.0%
!!! GAAAAGTAAGTTTTTTTAGC+TGG + tig0018106:53382-53401 Msa1364010:intron 25.0%
!! TTTGAGAGTTTCTTTTGTAT+TGG + tig0018106:53507-53526 Msa1364010:intron 25.0%
!!! TTTTAGTTTGACTGATTCTT+AGG + tig0018106:53644-53663 Msa1364010:intron 25.0%
!! ATTAAGTTGTTTATCCTAAC+AGG + tig0018106:53864-53883 Msa1364010:intron 25.0%
!! TTAAGTTGTTTATCCTAACA+GGG + tig0018106:53865-53884 Msa1364010:intron 25.0%
! ATCATAAACTATAGTACTCA+TGG - tig0018106:53907-53926 None:intergenic 25.0%
! AAAGTATACATGTCCTTAAA+AGG - tig0018106:53974-53993 None:intergenic 25.0%
!! CATTTTCAATATAAGGTATG+GGG + tig0018106:53999-54018 Msa1364010:intron 25.0%
! GATGCTAATATTATAGAAGA+AGG - tig0018106:54028-54047 None:intergenic 25.0%
!!! CTTTCATTTTTGTTGTTTCT+CGG + tig0018106:54108-54127 Msa1364010:intron 25.0%
!! CTTCATTCCTTTTGTAAATT+TGG + tig0018106:54133-54152 Msa1364010:intron 25.0%
! TCGAATACTACCTATAAATA+TGG + tig0018106:54187-54206 Msa1364010:intron 25.0%
! CGAATACTACCTATAAATAT+GGG + tig0018106:54188-54207 Msa1364010:intron 25.0%
! CAGAATAATCCCATATTTAT+AGG - tig0018106:54200-54219 None:intergenic 25.0%
!!! TAAAAAGTGTTGAACTTTTC+TGG + tig0018106:54258-54277 Msa1364010:intron 25.0%
!!! AAAAAGTGTTGAACTTTTCT+GGG + tig0018106:54259-54278 Msa1364010:intron 25.0%
! AAAACAAATTCATGCGTAAA+TGG - tig0018106:54560-54579 None:intergenic 25.0%
!!! AATTTTGAAAATGCAGAGTT+TGG + tig0018106:54625-54644 Msa1364010:intron 25.0%
! ATGTTCTCATAACTGATAAT+TGG + tig0018106:55337-55356 Msa1364010:three_prime_UTR 25.0%
ATTCAGAAAAACATGTTTGG+CGG - tig0018106:52862-52881 None:intergenic 30.0%
!! GCTGTTTTGTAAAGAGAATT+TGG - tig0018106:52918-52937 None:intergenic 30.0%
ATGACTTGAAATGAAACCTT+AGG - tig0018106:53045-53064 None:intergenic 30.0%
! AAGGTTTCATTTCAAGTCAT+TGG + tig0018106:53045-53064 Msa1364010:five_prime_UTR 30.0%
CTTCTTCAAATTGAACCAAA+AGG - tig0018106:53184-53203 None:intergenic 30.0%
TCACAAATTGATCCTGTTAA+AGG + tig0018106:53249-53268 Msa1364010:CDS 30.0%
GTATATTTGTGTAGCCTTTA+AGG + tig0018106:53550-53569 Msa1364010:intron 30.0%
!! ATAATTCTAAGGCTCTTGTT+TGG + tig0018106:53578-53597 Msa1364010:intron 30.0%
!! GTTAGCTTAATAGCCTTTTA+AGG + tig0018106:53958-53977 Msa1364010:intron 30.0%
GAATTTGTAACCAACTAAAG+AGG - tig0018106:54058-54077 None:intergenic 30.0%
CAAGCAACCAAATTTACAAA+AGG - tig0018106:54143-54162 None:intergenic 30.0%
! CTTTTGTAAATTTGGTTGCT+TGG + tig0018106:54141-54160 Msa1364010:intron 30.0%
!!! AAAAGTGTTGAACTTTTCTG+GGG + tig0018106:54260-54279 Msa1364010:intron 30.0%
GGTTAGAAATGGAAATGAAA+TGG + tig0018106:54281-54300 Msa1364010:intron 30.0%
AATGGAAATGAAATGGTTAC+TGG + tig0018106:54288-54307 Msa1364010:intron 30.0%
AACTGAAAATCTCTTTCTCA+AGG - tig0018106:54326-54345 None:intergenic 30.0%
TGAATTATACTACCCTTAGA+AGG - tig0018106:54496-54515 None:intergenic 30.0%
TTCTGTTTATGTTCACTTGA+TGG + tig0018106:54517-54536 Msa1364010:intron 30.0%
! AATTCACAGTGATAGCTTTT+AGG + tig0018106:54583-54602 Msa1364010:intron 30.0%
!!! AGTTTGGTTTTCTTGTTATG+AGG + tig0018106:54641-54660 Msa1364010:CDS 30.0%
!! AGGATGATTTTGAGGATAAA+AGG + tig0018106:54755-54774 Msa1364010:CDS 30.0%
TGAGGATAAAAGGAAAATGA+AGG + tig0018106:54765-54784 Msa1364010:CDS 30.0%
GAGGATAAAAGGAAAATGAA+GGG + tig0018106:54766-54785 Msa1364010:CDS 30.0%
GTATCTTTGATATCTCTCTT+AGG - tig0018106:54925-54944 None:intergenic 30.0%
AGATATCAAAGATACTAGTG+TGG + tig0018106:54930-54949 Msa1364010:CDS 30.0%
AACATAGTCCTAAACATACA+AGG - tig0018106:55269-55288 None:intergenic 30.0%
TAATTCTGAATGGAGTTCGA+AGG + tig0018106:52881-52900 Msa1364010:five_prime_UTR 35.0%
ACTTGAAATGAAACCTTAGG+AGG - tig0018106:53042-53061 None:intergenic 35.0%
TGTTCTTCAAAACCCAGATA+GGG - tig0018106:53077-53096 None:intergenic 35.0%
TTGTTCTTCAAAACCCAGAT+AGG - tig0018106:53078-53097 None:intergenic 35.0%
GAAATTCGAAATGGGTCATT+TGG + tig0018106:53122-53141 Msa1364010:exon 35.0%
! TTTTCGCAGACAAGCAAAAT+CGG - tig0018106:53158-53177 None:intergenic 35.0%
! ATAACTTTGACCCCTTTTGA+TGG + tig0018106:53274-53293 Msa1364010:intron 35.0%
TCCTGCTAAGAAAATGTCAT+TGG - tig0018106:53448-53467 None:intergenic 35.0%
! GGATATGAGTTGCAAGTTTT+AGG + tig0018106:53528-53547 Msa1364010:intron 35.0%
! CTCATGGTTGACTTAGATTT+AGG - tig0018106:53891-53910 None:intergenic 35.0%
! TTGTCTTTTGAACTTGAGTG+TGG + tig0018106:54234-54253 Msa1364010:intron 35.0%
! TGTCTTTTGAACTTGAGTGT+GGG + tig0018106:54235-54254 Msa1364010:intron 35.0%
!!! AACTTTTCTGGGGTTAGAAA+TGG + tig0018106:54270-54289 Msa1364010:intron 35.0%
AATCTCTTTCTCAAGGAATG+TGG - tig0018106:54319-54338 None:intergenic 35.0%
TGCTGAATTCATGTCAACAA+TGG + tig0018106:54362-54381 Msa1364010:intron 35.0%
TTATATCTTGCAAGTGTCTG+TGG + tig0018106:54462-54481 Msa1364010:intron 35.0%
!! TGATAAGGAACTTGTTGCTA+AGG + tig0018106:54846-54865 Msa1364010:CDS 35.0%
TCTATTGTCTTTAGACCTTG+CGG - tig0018106:54899-54918 None:intergenic 35.0%
TGTTACTGATGAAGAAGATG+AGG + tig0018106:55011-55030 Msa1364010:CDS 35.0%
GGAACAAAACAATGACAATG+AGG + tig0018106:55038-55057 Msa1364010:CDS 35.0%
AAACAATGACAATGAGGATG+AGG + tig0018106:55044-55063 Msa1364010:CDS 35.0%
CACTACATATCAACATCACA+AGG - tig0018106:55175-55194 None:intergenic 35.0%
! GAATGAAGCCTTGTATGTTT+AGG + tig0018106:55258-55277 Msa1364010:three_prime_UTR 35.0%
AACAGCGACAAAACACTGTT+CGG + tig0018106:52932-52951 Msa1364010:five_prime_UTR 40.0%
! CTGTTTCTCGTCATCACTTT+CGG + tig0018106:52987-53006 Msa1364010:five_prime_UTR 40.0%
AATCAGACTCGTTGTTGAGT+TGG - tig0018106:53014-53033 None:intergenic 40.0%
TCATTGGAAGAAACCCTATC+TGG + tig0018106:53061-53080 Msa1364010:five_prime_UTR 40.0%
CATTGGAAGAAACCCTATCT+GGG + tig0018106:53062-53081 Msa1364010:five_prime_UTR 40.0%
! GAAATGGGTCATTTGGAACT+TGG + tig0018106:53129-53148 Msa1364010:exon 40.0%
! GCCAATGACATTTTCTTAGC+AGG + tig0018106:53444-53463 Msa1364010:intron 40.0%
!! GCTATTTTCACAAGCTATCC+TGG - tig0018106:53624-53643 None:intergenic 40.0%
!! GCTATTTTCACAAGCTAGCA+TGG - tig0018106:53740-53759 None:intergenic 40.0%
ACTTAGATTTAGGCCCTGTT+AGG - tig0018106:53881-53900 None:intergenic 40.0%
ATCCTGCTTTCCTCTTTAGT+TGG + tig0018106:54045-54064 Msa1364010:intron 40.0%
AACCAACTAAAGAGGAAAGC+AGG - tig0018106:54050-54069 None:intergenic 40.0%
TGAGTTTGACCAGTATCCAA+AGG + tig0018106:54666-54685 Msa1364010:CDS 40.0%
GAGTTTGACCAGTATCCAAA+GGG + tig0018106:54667-54686 Msa1364010:CDS 40.0%
TCATCCTCACTTTCTTTGCT+AGG - tig0018106:54736-54755 None:intergenic 40.0%
TAGCAAAGAAAGTGAGGATG+AGG + tig0018106:54735-54754 Msa1364010:CDS 40.0%
! TGAGGATGAGGATGATTTTG+AGG + tig0018106:54747-54766 Msa1364010:CDS 40.0%
! TGGTGATAGTGACGATGATA+AGG + tig0018106:54831-54850 Msa1364010:CDS 40.0%
!! AAGGAACTTGTTGCTAAGGT+TGG + tig0018106:54850-54869 Msa1364010:CDS 40.0%
!! AGGAACTTGTTGCTAAGGTT+GGG + tig0018106:54851-54870 Msa1364010:CDS 40.0%
TTGGGAAAGGTAAGAAACCT+AGG + tig0018106:54869-54888 Msa1364010:CDS 40.0%
GCAAGGTCTAAAGACAATAG+AGG + tig0018106:54898-54917 Msa1364010:CDS 40.0%
TGATGAAGAAGATGAGGATG+AGG + tig0018106:55017-55036 Msa1364010:CDS 40.0%
ATATCAACATCACAAGGCCA+CGG - tig0018106:55169-55188 None:intergenic 40.0%
! AGTGATGACGAGAAACAGTG+TGG - tig0018106:52985-53004 None:intergenic 45.0%
! TGTCTGCGAAAACACCCTTT+TGG + tig0018106:53166-53185 Msa1364010:CDS 45.0%
TGTGGCTGTGTAACCTTCTA+AGG + tig0018106:54480-54499 Msa1364010:intron 45.0%
GTGGCTGTGTAACCTTCTAA+GGG + tig0018106:54481-54500 Msa1364010:intron 45.0%
! AGTATCCAAAGGGCATGATC+CGG + tig0018106:54677-54696 Msa1364010:CDS 45.0%
TGATCCGGAATGTGGAGAAT+TGG + tig0018106:54692-54711 Msa1364010:CDS 45.0%
GATCCGGAATGTGGAGAATT+GGG + tig0018106:54693-54712 Msa1364010:CDS 45.0%
ACTCCCAATTCTCCACATTC+CGG - tig0018106:54699-54718 None:intergenic 45.0%
GAGACAACAAAGATGACGAG+TGG + tig0018106:54806-54825 Msa1364010:CDS 45.0%
AACAAAGATGACGAGTGGAC+TGG + tig0018106:54811-54830 Msa1364010:CDS 45.0%
CTTGTTGCTAAGGTTGGGAA+AGG + tig0018106:54856-54875 Msa1364010:CDS 45.0%
TGACAATGAGGATGAGGAAG+AGG + tig0018106:55050-55069 Msa1364010:CDS 45.0%
AGAAGATGACGATGATGCTG+TGG + tig0018106:55095-55114 Msa1364010:CDS 45.0%
CTGTGGCGGTGTAAAATCAA+GGG + tig0018106:55112-55131 Msa1364010:exon 45.0%
TGTGGCGGTGTAAAATCAAG+GGG + tig0018106:55113-55132 Msa1364010:exon 45.0%
! TGTTTAGATACCAAGTGCCG+TGG + tig0018106:55149-55168 Msa1364010:three_prime_UTR 45.0%
ATGGAGTTCGAAGGCGAAGA+TGG + tig0018106:52890-52909 Msa1364010:five_prime_UTR 50.0%
GACAAAACACTGTTCGGACG+AGG + tig0018106:52938-52957 Msa1364010:five_prime_UTR 50.0%
GTCTGATTCAGAGCCTCCTA+AGG + tig0018106:53026-53045 Msa1364010:five_prime_UTR 50.0%
GGATCATGCCCTTTGGATAC+TGG - tig0018106:54678-54697 None:intergenic 50.0%
ACATTCCGGATCATGCCCTT+TGG - tig0018106:54685-54704 None:intergenic 50.0%
! AAAGGGCATGATCCGGAATG+TGG + tig0018106:54684-54703 Msa1364010:CDS 50.0%
GGAATGTGGAGAATTGGGAG+TGG + tig0018106:54698-54717 Msa1364010:CDS 50.0%
GAATTGGGAGTGGTTTCTCG+AGG + tig0018106:54708-54727 Msa1364010:CDS 50.0%
GGAGCCTAGCAAAGAAAGTG+AGG + tig0018106:54729-54748 Msa1364010:CDS 50.0%
AGAAACCTAGGTACTCCGCA+AGG + tig0018106:54881-54900 Msa1364010:CDS 50.0%
! TTAGACCTTGCGGAGTACCT+AGG - tig0018106:54889-54908 None:intergenic 50.0%
GATGACGATGATGAGACGCT+AGG + tig0018106:54979-54998 Msa1364010:CDS 50.0%
TGAGGATGAGGAAGAGGAAG+AGG + tig0018106:55056-55075 Msa1364010:CDS 50.0%
AGATGACGATGATGCTGTGG+CGG + tig0018106:55098-55117 Msa1364010:CDS 50.0%
GCTGTGGCGGTGTAAAATCA+AGG + tig0018106:55111-55130 Msa1364010:exon 50.0%
GTGGCGGTGTAAAATCAAGG+GGG + tig0018106:55114-55133 Msa1364010:exon 50.0%
ACACTGTTCGGACGAGGTTG+CGG + tig0018106:52944-52963 Msa1364010:five_prime_UTR 55.0%
GACGATGATGAGACGCTAGG+AGG + tig0018106:54982-55001 Msa1364010:CDS 55.0%
GGATGAGGAAGAGGAAGAGG+AGG + tig0018106:55059-55078 Msa1364010:CDS 55.0%
TGAGGAAGAGGAAGAGGAGG+AGG + tig0018106:55062-55081 Msa1364010:CDS 55.0%
CATCACAAGGCCACGGCACT+TGG - tig0018106:55162-55181 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0018106 gene 52841 55368 52841 ID=Msa1364010;Name=Msa1364010
tig0018106 mRNA 52841 55368 52841 ID=Msa1364010-mRNA-1;Parent=Msa1364010;Name=Msa1364010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=291|1|1|1|1|1|2|243|207
tig0018106 exon 52841 53270 52841 ID=Msa1364010-mRNA-1:exon:13774;Parent=Msa1364010-mRNA-1
tig0018106 exon 54641 55368 54641 ID=Msa1364010-mRNA-1:exon:13775;Parent=Msa1364010-mRNA-1
tig0018106 five_prime_UTR 52841 53131 52841 ID=Msa1364010-mRNA-1:five_prime_utr;Parent=Msa1364010-mRNA-1
tig0018106 CDS 53132 53270 53132 ID=Msa1364010-mRNA-1:cds;Parent=Msa1364010-mRNA-1
tig0018106 CDS 54641 55125 54641 ID=Msa1364010-mRNA-1:cds;Parent=Msa1364010-mRNA-1
tig0018106 three_prime_UTR 55126 55368 55126 ID=Msa1364010-mRNA-1:three_prime_utr;Parent=Msa1364010-mRNA-1
Gene Sequence

>Msa1364010

ATGGGTCATTTGGAACTTGGTGACCGATTTTGCTTGTCTGCGAAAACACCCTTTTGGTTCAATTTGAAGAAGAGTGAAGATTCTGAGAGTGAGATTGAATTTGATCAACTTGATATTTCACAAATTGATCCTGTTAAAGAGTTTGGTTTTCTTGTTATGAGGCATGAGTTTGACCAGTATCCAAAGGGCATGATCCGGAATGTGGAGAATTGGGAGTGGTTTCTCGAGGAGCCTAGCAAAGAAAGTGAGGATGAGGATGATTTTGAGGATAAAAGGAAAATGAAGGGAAAGCGAAAAGTATTTAGAGACAACAAAGATGACGAGTGGACTGGTGATAGTGACGATGATAAGGAACTTGTTGCTAAGGTTGGGAAAGGTAAGAAACCTAGGTACTCCGCAAGGTCTAAAGACAATAGAGGACCTAAGAGAGATATCAAAGATACTAGTGTGGATGAAAATGATGAAGAAGATGATGACGATGACGATGATGAGACGCTAGGAGGCTTCATTGTTACTGATGAAGAAGATGAGGATGAGGAACAAAACAATGACAATGAGGATGAGGAAGAGGAAGAGGAGGAGGAGTTTGAAGAAGAAGATGACGATGATGCTGTGGCGGTGTAA
Protein sequence

>Msa1364010

MGHLELGDRFCLSAKTPFWFNLKKSEDSESEIEFDQLDISQIDPVKEFGFLVMRHEFDQYPKGMIRNVENWEWFLEEPSKESEDEDDFEDKRKMKGKRKVFRDNKDDEWTGDSDDDKELVAKVGKGKKPRYSARSKDNRGPKRDIKDTSVDENDEEDDDDDDDETLGGFIVTDEEDEDEEQNNDNEDEEEEEEEEFEEEDDDDAVAV*