AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1365560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365560 MtrunA17_Chr5g0421951 79.859 283 30 5 2 260 1 280 1.24e-155 434
Msa1365560 MtrunA17_Chr5g0422001 74.377 281 39 6 2 255 1 275 9.06e-139 391
Msa1365560 MtrunA17_Chr6g0461801 62.500 200 38 6 2 187 1 177 5.14e-68 209
Msa1365560 MtrunA17_Chr6g0465351 83.077 65 11 0 159 223 3 67 9.46e-34 117
Msa1365560 MtrunA17_Chr6g0458841 47.368 95 33 3 51 128 1 95 1.97e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1365560 AT3G07930.4 40.945 254 114 6 21 259 95 327 1.83e-50 168
Msa1365560 AT3G07930.3 40.945 254 114 6 21 259 211 443 3.88e-49 168

Find 46 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTAACAATCTGTGTTTA+TGG 0.125925 tig0018369:-85194 None:intergenic
GGAAAACACTTGATAATAAC+TGG 0.253853 tig0018369:+82082 Msa1365560:CDS
CGAGAGTCATTGAAAATCTT+AGG 0.262380 tig0018369:-81970 None:intergenic
TCTTCGACTTTATGAAAATA+AGG 0.265794 tig0018369:-81814 None:intergenic
AACTGGGTGCCTCCACCTTC+TGG 0.292945 tig0018369:+82099 Msa1365560:CDS
TTGAAGATTTAGAACAGTTT+AGG 0.299387 tig0018369:+81890 Msa1365560:CDS
ATTATAAGCTGAACGAATAT+TGG 0.337988 tig0018369:+85150 Msa1365560:CDS
GAAGAGGTAATAAAGACATT+AGG 0.362333 tig0018369:+83519 Msa1365560:CDS
TTGAACAAGAGTGTAAAGTT+AGG 0.367789 tig0018369:+81782 Msa1365560:CDS
TGCATACAAGTTTCTGCGTC+TGG 0.405102 tig0018369:-83477 None:intergenic
TTCTTAAGGCTGATAGATGC+AGG 0.429544 tig0018369:+82046 Msa1365560:CDS
CATCTATCAGCCTTAAGAAA+AGG 0.442836 tig0018369:-82042 None:intergenic
CGTGGAAATGATACTCTTGA+AGG 0.486923 tig0018369:-82137 None:intergenic
TGAACAAGAGTGTAAAGTTA+GGG 0.506150 tig0018369:+81783 Msa1365560:CDS
TCGTTCAGCTTATAATCCCT+TGG 0.506473 tig0018369:-85143 None:intergenic
CATATAACTATCACTCTCCA+TGG 0.522838 tig0018369:-82162 None:intergenic
AGTATCATTTCCACGATCCA+TGG 0.535981 tig0018369:+82145 Msa1365560:CDS
GACTGCTGATGGTGGAAGGA+GGG 0.554805 tig0018369:+85253 Msa1365560:three_prime_UTR
AAGAAGAGAGGAAATAGAAG+AGG 0.558019 tig0018369:+83503 Msa1365560:CDS
GATTGTCGACGTACAAGTAA+TGG 0.559337 tig0018369:-81711 None:intergenic
GATGCAGGGAGGCTTACAAA+AGG 0.578219 tig0018369:+82061 Msa1365560:CDS
GTTACTGAACTGCACGGTGT+CGG 0.579421 tig0018369:+83612 Msa1365560:CDS
TCTTAAGGCTGATAGATGCA+GGG 0.581857 tig0018369:+82047 Msa1365560:CDS
GAACGAGACACGACCCTCTG+TGG 0.587983 tig0018369:-81856 None:intergenic
GGACTGCTGATGGTGGAAGG+AGG 0.589673 tig0018369:+85252 Msa1365560:three_prime_UTR
GTGAATACTTAACTGAGACT+TGG 0.590610 tig0018369:+83583 Msa1365560:CDS
GTGGGATGAAGTTGTGCCAA+GGG 0.598505 tig0018369:+85127 Msa1365560:CDS
CTATCACTCTCCATGGATCG+TGG 0.601633 tig0018369:-82155 None:intergenic
AAATGATACTCTTGAAGGAG+AGG 0.606210 tig0018369:-82132 None:intergenic
GGTGGACTGCTGATGGTGGA+AGG 0.610976 tig0018369:+85249 Msa1365560:three_prime_UTR
TTATACGCATACTTACTGAA+AGG 0.617301 tig0018369:-81733 None:intergenic
TTTATGGATGTCTTTCGCGA+AGG 0.621333 tig0018369:-85178 None:intergenic
TAAAGGTGGACTGCTGATGG+TGG 0.627702 tig0018369:+85245 Msa1365560:three_prime_UTR
AGTCGAAGAAGAGTGCAAAG+TGG 0.629579 tig0018369:+81828 Msa1365560:CDS
AATTCAAAACCAGAAGGTGG+AGG 0.629969 tig0018369:-82108 None:intergenic
CAAAGTGGAGCATCCACAGA+GGG 0.632129 tig0018369:+81843 Msa1365560:CDS
GAGTCATTGAAAATCTTAGG+AGG 0.634133 tig0018369:-81967 None:intergenic
ACCGTGCAGTTCAGTAACAT+AGG 0.643567 tig0018369:-83607 None:intergenic
AGTGGGATGAAGTTGTGCCA+AGG 0.644533 tig0018369:+85126 Msa1365560:CDS
AGAGGTAATTCAAAACCAGA+AGG 0.645372 tig0018369:-82114 None:intergenic
GGTAATTCAAAACCAGAAGG+TGG 0.647679 tig0018369:-82111 None:intergenic
GCAAAGTGGAGCATCCACAG+AGG 0.658092 tig0018369:+81842 Msa1365560:CDS
TTGTATGCAAGTAAGAAGAG+AGG 0.658799 tig0018369:+83491 Msa1365560:CDS
GAAAACACTTGATAATAACT+GGG 0.673694 tig0018369:+82083 Msa1365560:CDS
ACCTATGTTACTGAACTGCA+CGG 0.687032 tig0018369:+83606 Msa1365560:CDS
TAAGGCTGATAGATGCAGGG+AGG 0.729699 tig0018369:+82050 Msa1365560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAAAGAATATACTAAA+GGG + tig0018369:82990-83009 Msa1365560:intron 10.0%
!!! ATAATAAAATCCTTAATTTT+AGG - tig0018369:84252-84271 None:intergenic 10.0%
!!! TCTTTAAATTTTAACTTTTT+AGG + tig0018369:84771-84790 Msa1365560:intron 10.0%
!! AACATTAGCATAAAATATAA+GGG - tig0018369:82243-82262 None:intergenic 15.0%
!! AAACATTAGCATAAAATATA+AGG - tig0018369:82244-82263 None:intergenic 15.0%
!! AAAGCTATAATATCATAATA+AGG - tig0018369:82282-82301 None:intergenic 15.0%
!! TAGTAATTTAGAAATAAAGA+AGG + tig0018369:82761-82780 Msa1365560:intron 15.0%
!! AGTAATTTAGAAATAAAGAA+GGG + tig0018369:82762-82781 Msa1365560:intron 15.0%
!! CTAATAAAAGAATATACTAA+AGG + tig0018369:82989-83008 Msa1365560:intron 15.0%
!! AATAAAAGAATATACTAAAG+GGG + tig0018369:82991-83010 Msa1365560:intron 15.0%
!! TTTGAGAATATGTTAAATTA+GGG + tig0018369:83275-83294 Msa1365560:intron 15.0%
!! AATAACTAAAAAACACTAAA+GGG - tig0018369:83756-83775 None:intergenic 15.0%
!! AAATAACTAAAAAACACTAA+AGG - tig0018369:83757-83776 None:intergenic 15.0%
!! AATTGATTATAAGAATTTGA+GGG - tig0018369:83812-83831 None:intergenic 15.0%
!!! ATGATATGTATTTCTTTTAA+AGG + tig0018369:85228-85247 Msa1365560:three_prime_UTR 15.0%
!!! AACAATACTTAATTTTGATA+TGG + tig0018369:85368-85387 Msa1365560:three_prime_UTR 15.0%
!! TATAATATCATAATAAGGTC+AGG - tig0018369:82277-82296 None:intergenic 20.0%
!!! AGTCTTTAAAATAGAGAAAA+AGG - tig0018369:82648-82667 None:intergenic 20.0%
!! GTTTGAGAATATGTTAAATT+AGG + tig0018369:83274-83293 Msa1365560:intron 20.0%
!! AATATGTTAAATTAGGGAAT+TGG + tig0018369:83281-83300 Msa1365560:intron 20.0%
!!! ACTTTTAACAAGTTACTTTT+AGG + tig0018369:83678-83697 Msa1365560:intron 20.0%
!! CAATTGATTATAAGAATTTG+AGG - tig0018369:83813-83832 None:intergenic 20.0%
!!! AAAAAAAAAACCCTTTTTAG+TGG + tig0018369:83886-83905 Msa1365560:intron 20.0%
!! AATCATTCTCTAAATAATCT+TGG - tig0018369:84578-84597 None:intergenic 20.0%
!! AAGATTATTTAGAGAATGAT+TGG + tig0018369:84577-84596 Msa1365560:intron 20.0%
!!! ACAGTTAGTGTTTTTAAAAA+AGG - tig0018369:85016-85035 None:intergenic 20.0%
!!! TTCTTTACATTGATTAAGAT+TGG + tig0018369:85045-85064 Msa1365560:intron 20.0%
!! TAATTAACAATCTGTGTTTA+TGG - tig0018369:85197-85216 None:intergenic 20.0%
!!! ATATGTATTTCTTTTAAAGG+TGG + tig0018369:85231-85250 Msa1365560:three_prime_UTR 20.0%
!!! AACTATGTAGTTTTGTTTTT+TGG + tig0018369:85278-85297 Msa1365560:three_prime_UTR 20.0%
!!! TTTTTGGCAAAATACTAATA+TGG + tig0018369:85294-85313 Msa1365560:three_prime_UTR 20.0%
! TCTTCGACTTTATGAAAATA+AGG - tig0018369:81817-81836 None:intergenic 25.0%
!! TTGAAGATTTAGAACAGTTT+AGG + tig0018369:81890-81909 Msa1365560:CDS 25.0%
!!! AAAAGGTTTAGCTTTTCTTT+TGG - tig0018369:82028-82047 None:intergenic 25.0%
!! AAAAGCTAAACCTTTTCTTA+AGG + tig0018369:82032-82051 Msa1365560:CDS 25.0%
!! GAAAACACTTGATAATAACT+GGG + tig0018369:82083-82102 Msa1365560:CDS 25.0%
!! ATTTGAAATTTATCCTCTTG+AGG + tig0018369:82345-82364 Msa1365560:intron 25.0%
! GGACATTATGATTAAGAATT+TGG + tig0018369:82377-82396 Msa1365560:intron 25.0%
! GAATTTATGATAAAGAACAG+TGG + tig0018369:82619-82638 Msa1365560:intron 25.0%
!!! TTAATTTGTTACAGAACTAC+AGG - tig0018369:82733-82752 None:intergenic 25.0%
! TAAAGAAGGGATAATGTATA+AGG + tig0018369:82775-82794 Msa1365560:intron 25.0%
!! ATTTTCGCAGTAAATATGTT+AGG + tig0018369:82798-82817 Msa1365560:intron 25.0%
!!! TGAACAGTTTTTGAAATTCT+AGG - tig0018369:82862-82881 None:intergenic 25.0%
!! TTTCAAAAACTGTTCAGTTT+TGG + tig0018369:82866-82885 Msa1365560:intron 25.0%
!! AAGTTACACAATTTTTGTTG+AGG + tig0018369:83021-83040 Msa1365560:intron 25.0%
! ATGTACCATTTGCAATTTAT+TGG + tig0018369:83153-83172 Msa1365560:intron 25.0%
! ATAAGAAGCTAACTCAAAAT+AGG - tig0018369:83407-83426 None:intergenic 25.0%
! AAATAGCATAAACTACTAGA+GGG - tig0018369:83722-83741 None:intergenic 25.0%
!! GTTTATGCTATTTGTAACTT+TGG + tig0018369:83729-83748 Msa1365560:intron 25.0%
! ATGGAATGTACCTAAAATTA+AGG + tig0018369:84239-84258 Msa1365560:intron 25.0%
! ATATGGAATGAAGAATTACA+TGG + tig0018369:84341-84360 Msa1365560:intron 25.0%
!! GCTTGAGTTAAATTCAATTT+TGG - tig0018369:84968-84987 None:intergenic 25.0%
!!! TAGTGTTTTTAAAAAAGGCT+TGG - tig0018369:85011-85030 None:intergenic 25.0%
!!! GGTTATCATCTCTTTTAATT+AGG + tig0018369:85066-85085 Msa1365560:intron 25.0%
! ATTATAAGCTGAACGAATAT+TGG + tig0018369:85150-85169 Msa1365560:CDS 25.0%
TTATACGCATACTTACTGAA+AGG - tig0018369:81736-81755 None:intergenic 30.0%
! TTGAACAAGAGTGTAAAGTT+AGG + tig0018369:81782-81801 Msa1365560:CDS 30.0%
! TGAACAAGAGTGTAAAGTTA+GGG + tig0018369:81783-81802 Msa1365560:CDS 30.0%
! TTCATAGGCTTTTCAACTTT+CGG - tig0018369:82003-82022 None:intergenic 30.0%
! GCTTTTCTTTTGGATTTCAT+AGG - tig0018369:82018-82037 None:intergenic 30.0%
! GGAAAACACTTGATAATAAC+TGG + tig0018369:82082-82101 Msa1365560:CDS 30.0%
CTATCTAATTCACAGAGATT+AGG - tig0018369:82562-82581 None:intergenic 30.0%
TTGAAATTGAACGTGAATTC+TGG + tig0018369:82667-82686 Msa1365560:intron 30.0%
ATTAGTGTGTGATCATGTAT+AGG - tig0018369:82974-82993 None:intergenic 30.0%
AAAAGAATATACTAAAGGGG+AGG + tig0018369:82994-83013 Msa1365560:intron 30.0%
TAATAGAAGCAACAAGAAGA+TGG - tig0018369:83091-83110 None:intergenic 30.0%
TCACTCCAATAAATTGCAAA+TGG - tig0018369:83161-83180 None:intergenic 30.0%
GATTTGAGTAATGTCAAATG+AGG + tig0018369:83241-83260 Msa1365560:intron 30.0%
!!! CTTCTTATTTTGCAAAATGC+AGG + tig0018369:83419-83438 Msa1365560:intron 30.0%
GAAGAGGTAATAAAGACATT+AGG + tig0018369:83519-83538 Msa1365560:CDS 30.0%
! GAGGGTCTAAAATTGTAATT+TGG - tig0018369:83704-83723 None:intergenic 30.0%
CAAATAGCATAAACTACTAG+AGG - tig0018369:83723-83742 None:intergenic 30.0%
!!! GGGTTTTAAGCAAATGAAAA+GGG - tig0018369:83792-83811 None:intergenic 30.0%
!!! AGGGTTTTAAGCAAATGAAA+AGG - tig0018369:83793-83812 None:intergenic 30.0%
! AGAAGATGCTTAATGCTTTT+TGG + tig0018369:83909-83928 Msa1365560:intron 30.0%
AATGATTCAAACTCAACCAA+GGG - tig0018369:84161-84180 None:intergenic 30.0%
GAGTTTGAATCATTCTAAGT+TGG + tig0018369:84167-84186 Msa1365560:intron 30.0%
!!! CATCTCTCTCTCTTTATTTT+TGG + tig0018369:84298-84317 Msa1365560:intron 30.0%
TACATGGTAAATACATAGTG+AGG + tig0018369:84357-84376 Msa1365560:intron 30.0%
!! TAAGAATACAGATTTGCTGA+GGG - tig0018369:84472-84491 None:intergenic 30.0%
!! ATAAGAATACAGATTTGCTG+AGG - tig0018369:84473-84492 None:intergenic 30.0%
AGCAAATCTGTATTCTTATC+TGG + tig0018369:84474-84493 Msa1365560:intron 30.0%
! TTTAGGTTAAGTTTGTTCAG+AGG + tig0018369:84788-84807 Msa1365560:intron 30.0%
TTTATGTGAAATACAACCGA+TGG + tig0018369:84878-84897 Msa1365560:intron 30.0%
AATATAGGGACTAAGTTACT+CGG + tig0018369:84927-84946 Msa1365560:intron 30.0%
!! TAAATTCAATTTTGGTCCCT+CGG - tig0018369:84960-84979 None:intergenic 30.0%
!! TCTTTTAATTAGGTATGCTG+CGG + tig0018369:85076-85095 Msa1365560:intron 30.0%
! GCATATGCTATATTTTGTAC+AGG + tig0018369:85101-85120 Msa1365560:CDS 30.0%
! TATATTTTGTACAGGCAAGT+GGG + tig0018369:85109-85128 Msa1365560:CDS 30.0%
GAGTCATTGAAAATCTTAGG+AGG - tig0018369:81970-81989 None:intergenic 35.0%
CGAGAGTCATTGAAAATCTT+AGG - tig0018369:81973-81992 None:intergenic 35.0%
CATCTATCAGCCTTAAGAAA+AGG - tig0018369:82045-82064 None:intergenic 35.0%
AGAGGTAATTCAAAACCAGA+AGG - tig0018369:82117-82136 None:intergenic 35.0%
AAATGATACTCTTGAAGGAG+AGG - tig0018369:82135-82154 None:intergenic 35.0%
CATATAACTATCACTCTCCA+TGG - tig0018369:82165-82184 None:intergenic 35.0%
!! TCAAGAGGTTTTAGTAGAGA+AGG - tig0018369:82477-82496 None:intergenic 35.0%
AGGTTTGTGAGTAATTCAAG+AGG - tig0018369:82492-82511 None:intergenic 35.0%
TGGCAATGTTGTATATCAAG+AGG - tig0018369:82512-82531 None:intergenic 35.0%
TTCACAGAGATTAGGAGTAA+GGG - tig0018369:82554-82573 None:intergenic 35.0%
ATTCACAGAGATTAGGAGTA+AGG - tig0018369:82555-82574 None:intergenic 35.0%
TTTGTTACAGAACTACAGGA+AGG - tig0018369:82729-82748 None:intergenic 35.0%
!! TTGAAATTCTAGGCACTGAT+TGG - tig0018369:82852-82871 None:intergenic 35.0%
!!! CTGTTCAGTTTTGGATATAG+TGG + tig0018369:82875-82894 Msa1365560:intron 35.0%
GATCATGTATAGGACAGTTA+TGG - tig0018369:82964-82983 None:intergenic 35.0%
! TTTGTTGAGGTATGCTTTTG+CGG + tig0018369:83034-83053 Msa1365560:intron 35.0%
TTGAGTAATGTCAAATGAGG+TGG + tig0018369:83244-83263 Msa1365560:intron 35.0%
TTGTATGCAAGTAAGAAGAG+AGG + tig0018369:83491-83510 Msa1365560:CDS 35.0%
AAGAAGAGAGGAAATAGAAG+AGG + tig0018369:83503-83522 Msa1365560:CDS 35.0%
GTGAATACTTAACTGAGACT+TGG + tig0018369:83583-83602 Msa1365560:CDS 35.0%
AGCAAATGAAAAGGGATGAA+GGG - tig0018369:83784-83803 None:intergenic 35.0%
AAGCAAATGAAAAGGGATGA+AGG - tig0018369:83785-83804 None:intergenic 35.0%
AGCATCTTCTCCACTAAAAA+GGG - tig0018369:83899-83918 None:intergenic 35.0%
AAGCATCTTCTCCACTAAAA+AGG - tig0018369:83900-83919 None:intergenic 35.0%
!! AGATGCTTAATGCTTTTTGG+TGG + tig0018369:83912-83931 Msa1365560:intron 35.0%
!! GATGCTTAATGCTTTTTGGT+GGG + tig0018369:83913-83932 Msa1365560:intron 35.0%
!! ATGCTTAATGCTTTTTGGTG+GGG + tig0018369:83914-83933 Msa1365560:intron 35.0%
TTTCGTTGCTAGAATGTTGA+AGG + tig0018369:84090-84109 Msa1365560:intron 35.0%
GAATGATTCAAACTCAACCA+AGG - tig0018369:84162-84181 None:intergenic 35.0%
TCAAATCTTCGTGACATTGT+GGG - tig0018369:84200-84219 None:intergenic 35.0%
ATCAAATCTTCGTGACATTG+TGG - tig0018369:84201-84220 None:intergenic 35.0%
ACAATGTCACGAAGATTTGA+TGG + tig0018369:84200-84219 Msa1365560:intron 35.0%
AGATGTAATAGCTTCAGTCA+TGG - tig0018369:84283-84302 None:intergenic 35.0%
TTTGGAAGATCAAGATGACA+TGG + tig0018369:84316-84335 Msa1365560:intron 35.0%
ATCAAGATGACATGGTGATA+TGG + tig0018369:84324-84343 Msa1365560:intron 35.0%
TGGTAAATACATAGTGAGGT+CGG + tig0018369:84361-84380 Msa1365560:intron 35.0%
GTATTCTTATCTGGCAGATT+TGG + tig0018369:84483-84502 Msa1365560:intron 35.0%
TTGGAGAAAATGCTTACAGA+TGG + tig0018369:84502-84521 Msa1365560:intron 35.0%
TGGAGAAAATGCTTACAGAT+GGG + tig0018369:84503-84522 Msa1365560:intron 35.0%
ACTCAATACTAGTCACACAT+TGG - tig0018369:84614-84633 None:intergenic 35.0%
AAGAAATATGGTCCTACCAT+CGG - tig0018369:84897-84916 None:intergenic 35.0%
!! AAATTCAATTTTGGTCCCTC+GGG - tig0018369:84959-84978 None:intergenic 35.0%
!! AAAAGGCTTGGTTAGAGTTT+TGG - tig0018369:84999-85018 None:intergenic 35.0%
! CTATATTTTGTACAGGCAAG+TGG + tig0018369:85108-85127 Msa1365560:CDS 35.0%
!!! TGTTGCAGTTTTGTTGTAAG+TGG + tig0018369:85341-85360 Msa1365560:three_prime_UTR 35.0%
GATTGTCGACGTACAAGTAA+TGG - tig0018369:81714-81733 None:intergenic 40.0%
TTCTTAAGGCTGATAGATGC+AGG + tig0018369:82046-82065 Msa1365560:CDS 40.0%
TCTTAAGGCTGATAGATGCA+GGG + tig0018369:82047-82066 Msa1365560:CDS 40.0%
AATTCAAAACCAGAAGGTGG+AGG - tig0018369:82111-82130 None:intergenic 40.0%
GGTAATTCAAAACCAGAAGG+TGG - tig0018369:82114-82133 None:intergenic 40.0%
CGTGGAAATGATACTCTTGA+AGG - tig0018369:82140-82159 None:intergenic 40.0%
AGTATCATTTCCACGATCCA+TGG + tig0018369:82145-82164 Msa1365560:CDS 40.0%
CTGGTCTGCAATATTCTGTT+TGG + tig0018369:82686-82705 Msa1365560:intron 40.0%
TTTAGCAGACTCTTGATACG+CGG + tig0018369:82823-82842 Msa1365560:intron 40.0%
AGGACAGTTATGGTTATGTG+TGG - tig0018369:82954-82973 None:intergenic 40.0%
TCAATGACACGCAAGATGTT+GGG - tig0018369:83318-83337 None:intergenic 40.0%
ACCTATGTTACTGAACTGCA+CGG + tig0018369:83606-83625 Msa1365560:CDS 40.0%
ATTGGAGCAAAGGAATTCAG+TGG + tig0018369:83945-83964 Msa1365560:intron 40.0%
TTAGTCATGTTAGCCAAGCA+AGG + tig0018369:84040-84059 Msa1365560:intron 40.0%
TAGTCATGTTAGCCAAGCAA+GGG + tig0018369:84041-84060 Msa1365560:intron 40.0%
CTAGAATGTTGAAGGCGAAA+TGG + tig0018369:84098-84117 Msa1365560:intron 40.0%
TAGGGGATGTATAAAGGTGA+AGG + tig0018369:84125-84144 Msa1365560:intron 40.0%
CAAATCTTCGTGACATTGTG+GGG - tig0018369:84199-84218 None:intergenic 40.0%
! ATTTGATGGTTCCTGAAGAG+AGG + tig0018369:84214-84233 Msa1365560:intron 40.0%
GGTAAATACATAGTGAGGTC+GGG + tig0018369:84362-84381 Msa1365560:intron 40.0%
AATGCTTACAGATGGGAGAA+AGG + tig0018369:84510-84529 Msa1365560:intron 40.0%
AATCTTGGTACAACTCACAG+TGG - tig0018369:84563-84582 None:intergenic 40.0%
!! ATTGAGTGCTAGAGTATTGC+AGG + tig0018369:84627-84646 Msa1365560:intron 40.0%
CTGTGTCAAAATTCTGCAGA+CGG - tig0018369:84674-84693 None:intergenic 40.0%
!! CTGCAGAATTTTGACACAGT+TGG + tig0018369:84675-84694 Msa1365560:intron 40.0%
TGTGAAATACAACCGATGGT+AGG + tig0018369:84882-84901 Msa1365560:intron 40.0%
TCGTTCAGCTTATAATCCCT+TGG - tig0018369:85146-85165 None:intergenic 40.0%
TTTATGGATGTCTTTCGCGA+AGG - tig0018369:85181-85200 None:intergenic 40.0%
!!! TTTTAAAGGTGGACTGCTGA+TGG + tig0018369:85242-85261 Msa1365560:three_prime_UTR 40.0%
! AGTCGAAGAAGAGTGCAAAG+TGG + tig0018369:81828-81847 Msa1365560:CDS 45.0%
ATCCTCTTGAGGTCTTGCTT+AGG + tig0018369:82356-82375 Msa1365560:intron 45.0%
! GCTGGCAAGACTATTTTGTG+TGG - tig0018369:82532-82551 None:intergenic 45.0%
TGTCAAATGAGGTGGATGCT+TGG + tig0018369:83252-83271 Msa1365560:intron 45.0%
GTCAATGACACGCAAGATGT+TGG - tig0018369:83319-83338 None:intergenic 45.0%
TGCATACAAGTTTCTGCGTC+TGG - tig0018369:83480-83499 None:intergenic 45.0%
ACCGTGCAGTTCAGTAACAT+AGG - tig0018369:83610-83629 None:intergenic 45.0%
!! CTTAATGCTTTTTGGTGGGG+AGG + tig0018369:83917-83936 Msa1365560:intron 45.0%
GAGCAAAGGAATTCAGTGGT+TGG + tig0018369:83949-83968 Msa1365560:intron 45.0%
!! AGGAATTCAGTGGTTGGCTT+AGG + tig0018369:83955-83974 Msa1365560:intron 45.0%
CCTATCCCTCTCTAAGCTTT+GGG - tig0018369:83995-84014 None:intergenic 45.0%
ACCTATCCCTCTCTAAGCTT+TGG - tig0018369:83996-84015 None:intergenic 45.0%
TAGCATATTCCACCCTTGCT+TGG - tig0018369:84056-84075 None:intergenic 45.0%
GTGGAATATGCTATGCAAGC+TGG + tig0018369:84063-84082 Msa1365560:intron 45.0%
GAATGTTGAAGGCGAAATGG+CGG + tig0018369:84101-84120 Msa1365560:intron 45.0%
ACATTCCATCTCCTCTCTTC+AGG - tig0018369:84228-84247 None:intergenic 45.0%
AGAAGAAACGCTCTGTGCAA+GGG + tig0018369:84417-84436 Msa1365560:intron 45.0%
!! ACAGATTTGCTGAGGGACAT+GGG - tig0018369:84465-84484 None:intergenic 45.0%
!! TACAGATTTGCTGAGGGACA+TGG - tig0018369:84466-84485 None:intergenic 45.0%
TACAGATGGGAGAAAGGTTG+CGG + tig0018369:84516-84535 Msa1365560:intron 45.0%
TGGTACAACTCACAGTGGTT+CGG - tig0018369:84558-84577 None:intergenic 45.0%
AGTTACTCGGAAAAAACCCG+AGG + tig0018369:84940-84959 Msa1365560:intron 45.0%
GTTACTCGGAAAAAACCCGA+GGG + tig0018369:84941-84960 Msa1365560:intron 45.0%
!!! TTTTAAAAATTAAAGAAATA+TGG - tig0018369:84909-84928 None:intergenic 5.0%
!!! TTCTTTAATTTTTAAAATAT+AGG + tig0018369:84912-84931 Msa1365560:intron 5.0%
!!! TCTTTAATTTTTAAAATATA+GGG + tig0018369:84913-84932 Msa1365560:intron 5.0%
! CAAAGTGGAGCATCCACAGA+GGG + tig0018369:81843-81862 Msa1365560:CDS 50.0%
TAAGGCTGATAGATGCAGGG+AGG + tig0018369:82050-82069 Msa1365560:CDS 50.0%
GATGCAGGGAGGCTTACAAA+AGG + tig0018369:82061-82080 Msa1365560:CDS 50.0%
CTATCACTCTCCATGGATCG+TGG - tig0018369:82158-82177 None:intergenic 50.0%
GTCCTAAGCAAGACCTCAAG+AGG - tig0018369:82361-82380 None:intergenic 50.0%
CAGAGATTAGGAGTAAGGGC+TGG - tig0018369:82550-82569 None:intergenic 50.0%
GTTACTGAACTGCACGGTGT+CGG + tig0018369:83612-83631 Msa1365560:CDS 50.0%
!! AATGCTTTTTGGTGGGGAGG+AGG + tig0018369:83920-83939 Msa1365560:intron 50.0%
TTTGGTGGGGAGGAGGTAAT+TGG + tig0018369:83927-83946 Msa1365560:intron 50.0%
GGAGGAGGTAATTGGAGCAA+AGG + tig0018369:83935-83954 Msa1365560:intron 50.0%
TCGTGTCCCAAAGCTTAGAG+AGG + tig0018369:83986-84005 Msa1365560:intron 50.0%
CGTGTCCCAAAGCTTAGAGA+GGG + tig0018369:83987-84006 Msa1365560:intron 50.0%
CCCAAAGCTTAGAGAGGGAT+AGG + tig0018369:83992-84011 Msa1365560:intron 50.0%
TCATGTTAGCCAAGCAAGGG+TGG + tig0018369:84044-84063 Msa1365560:intron 50.0%
!! TTGAAGGCGAAATGGCGGAT+AGG + tig0018369:84106-84125 Msa1365560:intron 50.0%
!! TGAAGGCGAAATGGCGGATA+GGG + tig0018369:84107-84126 Msa1365560:intron 50.0%
GGCGGATAGGGGATGTATAA+AGG + tig0018369:84119-84138 Msa1365560:intron 50.0%
TGAAGGTCATGCAAGACCCT+TGG + tig0018369:84142-84161 Msa1365560:intron 50.0%
TGGTTCCTGAAGAGAGGAGA+TGG + tig0018369:84220-84239 Msa1365560:intron 50.0%
GAGAAGAAACGCTCTGTGCA+AGG + tig0018369:84416-84435 Msa1365560:intron 50.0%
ACAGATGGGAGAAAGGTTGC+GGG + tig0018369:84517-84536 Msa1365560:intron 50.0%
CACAGTTGGAGCTGCGTTAT+TGG + tig0018369:84689-84708 Msa1365560:intron 50.0%
TGTTCAGAGGCAAGCGAACA+TGG + tig0018369:84801-84820 Msa1365560:intron 50.0%
AGTGGGATGAAGTTGTGCCA+AGG + tig0018369:85126-85145 Msa1365560:CDS 50.0%
GTGGGATGAAGTTGTGCCAA+GGG + tig0018369:85127-85146 Msa1365560:CDS 50.0%
!! TAAAGGTGGACTGCTGATGG+TGG + tig0018369:85245-85264 Msa1365560:three_prime_UTR 50.0%
! GCAAAGTGGAGCATCCACAG+AGG + tig0018369:81842-81861 Msa1365560:CDS 55.0%
!! GAAGGCGAAATGGCGGATAG+GGG + tig0018369:84108-84127 Msa1365560:intron 55.0%
GCTCTGTGCAAGGGAAGTGA+CGG + tig0018369:84426-84445 Msa1365560:intron 55.0%
! AAGGGAAGTGACGGAGGTTG+TGG + tig0018369:84435-84454 Msa1365560:intron 55.0%
! GAAGTGACGGAGGTTGTGGA+AGG + tig0018369:84439-84458 Msa1365560:intron 55.0%
!! GACTGCTGATGGTGGAAGGA+GGG + tig0018369:85253-85272 Msa1365560:three_prime_UTR 55.0%
GAACGAGACACGACCCTCTG+TGG - tig0018369:81859-81878 None:intergenic 60.0%
AACTGGGTGCCTCCACCTTC+TGG + tig0018369:82099-82118 Msa1365560:CDS 60.0%
CTGTGCAAGGGAAGTGACGG+AGG + tig0018369:84429-84448 Msa1365560:intron 60.0%
!! GGTGGACTGCTGATGGTGGA+AGG + tig0018369:85249-85268 Msa1365560:three_prime_UTR 60.0%
!! GGACTGCTGATGGTGGAAGG+AGG + tig0018369:85252-85271 Msa1365560:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
tig0018369 gene 81697 85415 81697 ID=Msa1365560;Name=Msa1365560
tig0018369 mRNA 81697 85415 81697 ID=Msa1365560-mRNA-1;Parent=Msa1365560;Name=Msa1365560-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.5|0.66|1|0.5|1|3|216|260
tig0018369 exon 81697 82170 81697 ID=Msa1365560-mRNA-1:exon:14617;Parent=Msa1365560-mRNA-1
tig0018369 exon 83441 83637 83441 ID=Msa1365560-mRNA-1:exon:14618;Parent=Msa1365560-mRNA-1
tig0018369 exon 85088 85415 85088 ID=Msa1365560-mRNA-1:exon:14619;Parent=Msa1365560-mRNA-1
tig0018369 CDS 81697 82170 81697 ID=Msa1365560-mRNA-1:cds;Parent=Msa1365560-mRNA-1
tig0018369 CDS 83441 83637 83441 ID=Msa1365560-mRNA-1:cds;Parent=Msa1365560-mRNA-1
tig0018369 CDS 85088 85199 85088 ID=Msa1365560-mRNA-1:cds;Parent=Msa1365560-mRNA-1
tig0018369 three_prime_UTR 85200 85415 85200 ID=Msa1365560-mRNA-1:three_prime_utr;Parent=Msa1365560-mRNA-1
Gene Sequence

>Msa1365560

AGAATGGAAAGTAACCATTACTTGTACGTCGACAATCCTTTCAGTAAGTATGCGTATAAGCGAAATTATCTTTCAAAGAGTGAAGTTGAACAAGAGTGTAAAGTTAGGGTCGTGTCTCCTTATTTTCATAAAGTCGAAGAAGAGTGCAAAGTGGAGCATCCACAGAGGGTCGTGTCTCGTTCTACGAAGAGCATTGAAGATTTAGAACAGTTTAGGTATCAGAGTGAAAAAGTGTCAAGTCTAAGAGAGTGTGTTAAGAACAGTGAACTTCCTCCTAAGATTTTCAATGACTCTCGAAAGAGACCGAAAGTTGAAAAGCCTATGAAATCCAAAAGAAAAGCTAAACCTTTTCTTAAGGCTGATAGATGCAGGGAGGCTTACAAAAGGAAAACACTTGATAATAACTGGGTGCCTCCACCTTCTGGTTTTGAATTACCTCTCCTTCAAGAGTATCATTTCCACGATCCATGGAGAGCAGAACAAGTTTTAGATAACTTTTTTGAGTTATGTCCAGACGCAGAAACTTGTATGCAAGTAAGAAGAGAGGAAATAGAAGAGGTAATAAAGACATTAGGTTTTCAAGTGAAAAGATCAAGAAGTTTGCAACGCTTTTCTCGTGAATACTTAACTGAGACTTGGACCTATGTTACTGAACTGCACGGTGTCGGCAAGTATGCTGCGGATGCATATGCTATATTTTGTACAGGCAAGTGGGATGAAGTTGTGCCAAGGGATTATAAGCTGAACGAATATTGGAATTTCCTTCGCGAAAGACATCCATAA
Protein sequence

>Msa1365560

RMESNHYLYVDNPFSKYAYKRNYLSKSEVEQECKVRVVSPYFHKVEEECKVEHPQRVVSRSTKSIEDLEQFRYQSEKVSSLRECVKNSELPPKIFNDSRKRPKVEKPMKSKRKAKPFLKADRCREAYKRKTLDNNWVPPPSGFELPLLQEYHFHDPWRAEQVLDNFFELCPDAETCMQVRREEIEEVIKTLGFQVKRSRSLQRFSREYLTETWTYVTELHGVGKYAADAYAIFCTGKWDEVVPRDYKLNEYWNFLRERHP*