AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0662410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662410 MtrunA17_Chr5g0421951 80.755 265 24 5 5 245 19 280 4.49e-147 412
Msa0662410 MtrunA17_Chr5g0422001 75.379 264 37 5 4 240 13 275 3.36e-133 377
Msa0662410 MtrunA17_Chr6g0461801 61.828 186 34 6 1 172 15 177 9.83e-60 187
Msa0662410 MtrunA17_Chr6g0465351 83.077 65 11 0 144 208 3 67 7.82e-34 117
Msa0662410 MtrunA17_Chr6g0458841 47.368 95 33 3 36 113 1 95 1.93e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662410 AT3G07930.4 53.333 150 53 2 110 244 180 327 1.84e-49 165
Msa0662410 AT3G07930.3 53.333 150 53 2 110 244 296 443 3.13e-48 165

Find 43 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTAACAATCTGTGTTTA+TGG 0.125925 4_4:-78331 None:intergenic
GGAAAACACTTGATAATAAC+TGG 0.253853 4_4:+75220 Msa0662410:CDS
CGAGAGTCATTGAAAATCTT+AGG 0.262380 4_4:-75108 None:intergenic
AACTGGGTGCCTCCACCTTC+TGG 0.292945 4_4:+75237 Msa0662410:CDS
TTGAAGATTTAGAACAGTTT+AGG 0.299387 4_4:+75028 Msa0662410:CDS
ATTATAAGCTGAACGAATAT+TGG 0.337988 4_4:+78287 Msa0662410:CDS
GAAGAGGTAATAAAGACATT+AGG 0.362333 4_4:+76656 Msa0662410:CDS
TTGAACAAGAGTGTAAAGTT+AGG 0.367789 4_4:+74920 Msa0662410:CDS
TGCATACAAGTTTCTGCGTC+TGG 0.405102 4_4:-76614 None:intergenic
TTCTTAAGGCTGATAGATGC+AGG 0.429544 4_4:+75184 Msa0662410:CDS
CATCTATCAGCCTTAAGAAA+AGG 0.442836 4_4:-75180 None:intergenic
CGTGGAAATGATACTCTTGA+AGG 0.486923 4_4:-75275 None:intergenic
TGAACAAGAGTGTAAAGTTA+GGG 0.506150 4_4:+74921 Msa0662410:CDS
TCGTTCAGCTTATAATCCCT+TGG 0.506473 4_4:-78280 None:intergenic
CATATAACTATCACTCTCCA+TGG 0.522838 4_4:-75300 None:intergenic
AGTATCATTTCCACGATCCA+TGG 0.535981 4_4:+75283 Msa0662410:CDS
GACTGCTGATGGTGGAAGGA+GGG 0.554805 4_4:+78390 Msa0662410:three_prime_UTR
AAGAAGAGAGGAAATAGAAG+AGG 0.558019 4_4:+76640 Msa0662410:CDS
GATGCAGGGAGGCTTACAAA+AGG 0.578219 4_4:+75199 Msa0662410:CDS
GTTACTGAACTGCACGGTGT+CGG 0.579421 4_4:+76749 Msa0662410:CDS
TCTTAAGGCTGATAGATGCA+GGG 0.581857 4_4:+75185 Msa0662410:CDS
GAACGAGACACGACCCTCTG+TGG 0.587983 4_4:-74994 None:intergenic
GGACTGCTGATGGTGGAAGG+AGG 0.589673 4_4:+78389 Msa0662410:three_prime_UTR
GTGAATACTTAACTGAGACT+TGG 0.590610 4_4:+76720 Msa0662410:CDS
GTGGGATGAAGTTGTGCCAA+GGG 0.598505 4_4:+78264 Msa0662410:CDS
CTATCACTCTCCATGGATCG+TGG 0.601633 4_4:-75293 None:intergenic
AAATGATACTCTTGAAGGAG+AGG 0.606210 4_4:-75270 None:intergenic
GGTGGACTGCTGATGGTGGA+AGG 0.610976 4_4:+78386 Msa0662410:three_prime_UTR
TTTATGGATGTCTTTCGCGA+AGG 0.621333 4_4:-78315 None:intergenic
TAAAGGTGGACTGCTGATGG+TGG 0.627702 4_4:+78382 Msa0662410:three_prime_UTR
AGTCGAAGAAGAGTGCAAAG+TGG 0.629579 4_4:+74966 Msa0662410:CDS
AATTCAAAACCAGAAGGTGG+AGG 0.629969 4_4:-75246 None:intergenic
CAAAGTGGAGCATCCACAGA+GGG 0.632129 4_4:+74981 Msa0662410:CDS
GAGTCATTGAAAATCTTAGG+AGG 0.634133 4_4:-75105 None:intergenic
ACCGTGCAGTTCAGTAACAT+AGG 0.643567 4_4:-76744 None:intergenic
AGTGGGATGAAGTTGTGCCA+AGG 0.644533 4_4:+78263 Msa0662410:CDS
AGAGGTAATTCAAAACCAGA+AGG 0.645372 4_4:-75252 None:intergenic
GGTAATTCAAAACCAGAAGG+TGG 0.647679 4_4:-75249 None:intergenic
GCAAAGTGGAGCATCCACAG+AGG 0.658092 4_4:+74980 Msa0662410:CDS
TTGTATGCAAGTAAGAAGAG+AGG 0.658799 4_4:+76628 Msa0662410:CDS
GAAAACACTTGATAATAACT+GGG 0.673694 4_4:+75221 Msa0662410:CDS
ACCTATGTTACTGAACTGCA+CGG 0.687032 4_4:+76743 Msa0662410:CDS
TAAGGCTGATAGATGCAGGG+AGG 0.729699 4_4:+75188 Msa0662410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAAAGAATATACTAAA+GGG + chr4_4:76127-76146 Msa0662410:intron 10.0%
!!! ATAATAAAATCCTTAATTTT+AGG - chr4_4:77389-77408 None:intergenic 10.0%
!!! TCTTTAAATTTTAACTTTTT+AGG + chr4_4:77908-77927 Msa0662410:intron 10.0%
!! AACATTAGCATAAAATATAA+GGG - chr4_4:75379-75398 None:intergenic 15.0%
!! AAACATTAGCATAAAATATA+AGG - chr4_4:75380-75399 None:intergenic 15.0%
!! AAAGCTATAATATCATAATA+AGG - chr4_4:75419-75438 None:intergenic 15.0%
!! TAGTAATTTAGAAATAAAGA+AGG + chr4_4:75898-75917 Msa0662410:intron 15.0%
!! AGTAATTTAGAAATAAAGAA+GGG + chr4_4:75899-75918 Msa0662410:intron 15.0%
!! CTAATAAAAGAATATACTAA+AGG + chr4_4:76126-76145 Msa0662410:intron 15.0%
!! AATAAAAGAATATACTAAAG+GGG + chr4_4:76128-76147 Msa0662410:intron 15.0%
!! TTTGAGAATATGTTAAATTA+GGG + chr4_4:76412-76431 Msa0662410:intron 15.0%
!! AATAACTAAAAAACACTAAA+GGG - chr4_4:76893-76912 None:intergenic 15.0%
!! AAATAACTAAAAAACACTAA+AGG - chr4_4:76894-76913 None:intergenic 15.0%
!! AATTGATTATAAGAATTTGA+GGG - chr4_4:76949-76968 None:intergenic 15.0%
!!! ATGATATGTATTTCTTTTAA+AGG + chr4_4:78365-78384 Msa0662410:three_prime_UTR 15.0%
!!! AACAATACTTAATTTTGATA+TGG + chr4_4:78505-78524 Msa0662410:three_prime_UTR 15.0%
!! TATAATATCATAATAAGGTC+AGG - chr4_4:75414-75433 None:intergenic 20.0%
!!! AGTCTTTAAAATAGAGAAAA+AGG - chr4_4:75785-75804 None:intergenic 20.0%
!! GTTTGAGAATATGTTAAATT+AGG + chr4_4:76411-76430 Msa0662410:intron 20.0%
!! AATATGTTAAATTAGGGAAT+TGG + chr4_4:76418-76437 Msa0662410:intron 20.0%
!!! ACTTTTAACAAGTTACTTTT+AGG + chr4_4:76815-76834 Msa0662410:intron 20.0%
!! CAATTGATTATAAGAATTTG+AGG - chr4_4:76950-76969 None:intergenic 20.0%
!!! AAAAAAAAAACCCTTTTTAG+TGG + chr4_4:77023-77042 Msa0662410:intron 20.0%
!! AATCATTCTCTAAATAATCT+TGG - chr4_4:77715-77734 None:intergenic 20.0%
!! AAGATTATTTAGAGAATGAT+TGG + chr4_4:77714-77733 Msa0662410:intron 20.0%
!!! ACAGTTAGTGTTTTTAAAAA+AGG - chr4_4:78153-78172 None:intergenic 20.0%
!!! TTCTTTACATTGATTAAGAT+TGG + chr4_4:78182-78201 Msa0662410:intron 20.0%
!! TAATTAACAATCTGTGTTTA+TGG - chr4_4:78334-78353 None:intergenic 20.0%
!!! ATATGTATTTCTTTTAAAGG+TGG + chr4_4:78368-78387 Msa0662410:three_prime_UTR 20.0%
!!! AACTATGTAGTTTTGTTTTT+TGG + chr4_4:78415-78434 Msa0662410:three_prime_UTR 20.0%
!!! TTTTTGGCAAAATACTAATA+TGG + chr4_4:78431-78450 Msa0662410:three_prime_UTR 20.0%
!! TTGAAGATTTAGAACAGTTT+AGG + chr4_4:75028-75047 Msa0662410:CDS 25.0%
!!! AAAAGGTTTAGCTTTTCTTT+TGG - chr4_4:75166-75185 None:intergenic 25.0%
!! AAAAGCTAAACCTTTTCTTA+AGG + chr4_4:75170-75189 Msa0662410:CDS 25.0%
!! GAAAACACTTGATAATAACT+GGG + chr4_4:75221-75240 Msa0662410:CDS 25.0%
!! ATTTGAAATTTATCCTCTTG+AGG + chr4_4:75482-75501 Msa0662410:intron 25.0%
! GGACATTATGATTAAGAATT+TGG + chr4_4:75514-75533 Msa0662410:intron 25.0%
! GAATTTATGATAAAGAACAG+TGG + chr4_4:75756-75775 Msa0662410:intron 25.0%
!!! TTAATTTGTTACAGAACTAC+AGG - chr4_4:75870-75889 None:intergenic 25.0%
! TAAAGAAGGGATAATGTATA+AGG + chr4_4:75912-75931 Msa0662410:intron 25.0%
!! ATTTTCGCAGTAAATATGTT+AGG + chr4_4:75935-75954 Msa0662410:intron 25.0%
!!! TGAACAGTTTTTGAAATTCT+AGG - chr4_4:75999-76018 None:intergenic 25.0%
!! TTTCAAAAACTGTTCAGTTT+TGG + chr4_4:76003-76022 Msa0662410:intron 25.0%
!! AAGTTACACAATTTTTGTTG+AGG + chr4_4:76158-76177 Msa0662410:intron 25.0%
! ATGTACCATTTGCAATTTAT+TGG + chr4_4:76290-76309 Msa0662410:intron 25.0%
! ATAAGAAGCTAACTCAAAAT+AGG - chr4_4:76544-76563 None:intergenic 25.0%
! AAATAGCATAAACTACTAGA+GGG - chr4_4:76859-76878 None:intergenic 25.0%
!! GTTTATGCTATTTGTAACTT+TGG + chr4_4:76866-76885 Msa0662410:intron 25.0%
! ATGGAATGTACCTAAAATTA+AGG + chr4_4:77376-77395 Msa0662410:intron 25.0%
! ATATGGAATGAAGAATTACA+TGG + chr4_4:77478-77497 Msa0662410:intron 25.0%
!! GCTTGAGTTAAATTCAATTT+TGG - chr4_4:78105-78124 None:intergenic 25.0%
!!! TAGTGTTTTTAAAAAAGGCT+TGG - chr4_4:78148-78167 None:intergenic 25.0%
!!! GGTTATCATCTCTTTTAATT+AGG + chr4_4:78203-78222 Msa0662410:intron 25.0%
! ATTATAAGCTGAACGAATAT+TGG + chr4_4:78287-78306 Msa0662410:CDS 25.0%
! TTGAACAAGAGTGTAAAGTT+AGG + chr4_4:74920-74939 Msa0662410:CDS 30.0%
! TGAACAAGAGTGTAAAGTTA+GGG + chr4_4:74921-74940 Msa0662410:CDS 30.0%
! TTCATAGGCTTTTCAACTTT+CGG - chr4_4:75141-75160 None:intergenic 30.0%
! GCTTTTCTTTTGGATTTCAT+AGG - chr4_4:75156-75175 None:intergenic 30.0%
! GGAAAACACTTGATAATAAC+TGG + chr4_4:75220-75239 Msa0662410:CDS 30.0%
GAGAGTGATAGTTATATGTA+TGG + chr4_4:75305-75324 Msa0662410:intron 30.0%
TATGTATGGAGTGAAGTTAT+AGG + chr4_4:75319-75338 Msa0662410:intron 30.0%
CTATCTAATTCACAGAGATT+AGG - chr4_4:75699-75718 None:intergenic 30.0%
TTGAAATTGAACGTGAATTC+TGG + chr4_4:75804-75823 Msa0662410:intron 30.0%
ATTAGTGTGTGATCATGTAT+AGG - chr4_4:76111-76130 None:intergenic 30.0%
AAAAGAATATACTAAAGGGG+AGG + chr4_4:76131-76150 Msa0662410:intron 30.0%
TAATAGAAGCAACAAGAAGA+TGG - chr4_4:76228-76247 None:intergenic 30.0%
TCACTCCAATAAATTGCAAA+TGG - chr4_4:76298-76317 None:intergenic 30.0%
GATTTGAGTAATGTCAAATG+AGG + chr4_4:76378-76397 Msa0662410:intron 30.0%
!!! CTTCTTATTTTGCAAAATGC+AGG + chr4_4:76556-76575 Msa0662410:intron 30.0%
GAAGAGGTAATAAAGACATT+AGG + chr4_4:76656-76675 Msa0662410:CDS 30.0%
! GAGGGTCTAAAATTGTAATT+TGG - chr4_4:76841-76860 None:intergenic 30.0%
CAAATAGCATAAACTACTAG+AGG - chr4_4:76860-76879 None:intergenic 30.0%
!!! GGGTTTTAAGCAAATGAAAA+GGG - chr4_4:76929-76948 None:intergenic 30.0%
!!! AGGGTTTTAAGCAAATGAAA+AGG - chr4_4:76930-76949 None:intergenic 30.0%
! AGAAGATGCTTAATGCTTTT+TGG + chr4_4:77046-77065 Msa0662410:intron 30.0%
AATGATTCAAACTCAACCAA+GGG - chr4_4:77298-77317 None:intergenic 30.0%
GAGTTTGAATCATTCTAAGT+TGG + chr4_4:77304-77323 Msa0662410:intron 30.0%
!!! CATCTCTCTCTCTTTATTTT+TGG + chr4_4:77435-77454 Msa0662410:intron 30.0%
TACATGGTAAATACATAGTG+AGG + chr4_4:77494-77513 Msa0662410:intron 30.0%
!! TAAGAATACAGATTTGCTGA+GGG - chr4_4:77609-77628 None:intergenic 30.0%
!! ATAAGAATACAGATTTGCTG+AGG - chr4_4:77610-77629 None:intergenic 30.0%
AGCAAATCTGTATTCTTATC+TGG + chr4_4:77611-77630 Msa0662410:intron 30.0%
! TTTAGGTTAAGTTTGTTCAG+AGG + chr4_4:77925-77944 Msa0662410:intron 30.0%
TTTATGTGAAATACAACCGA+TGG + chr4_4:78015-78034 Msa0662410:intron 30.0%
AATATAGGGACTAAGTTACT+CGG + chr4_4:78064-78083 Msa0662410:intron 30.0%
!! TAAATTCAATTTTGGTCCCT+CGG - chr4_4:78097-78116 None:intergenic 30.0%
!! TCTTTTAATTAGGTATGCTG+CGG + chr4_4:78213-78232 Msa0662410:intron 30.0%
! GCATATGCTATATTTTGTAC+AGG + chr4_4:78238-78257 Msa0662410:CDS 30.0%
! TATATTTTGTACAGGCAAGT+GGG + chr4_4:78246-78265 Msa0662410:CDS 30.0%
GAGTCATTGAAAATCTTAGG+AGG - chr4_4:75108-75127 None:intergenic 35.0%
CGAGAGTCATTGAAAATCTT+AGG - chr4_4:75111-75130 None:intergenic 35.0%
CATCTATCAGCCTTAAGAAA+AGG - chr4_4:75183-75202 None:intergenic 35.0%
AGAGGTAATTCAAAACCAGA+AGG - chr4_4:75255-75274 None:intergenic 35.0%
AAATGATACTCTTGAAGGAG+AGG - chr4_4:75273-75292 None:intergenic 35.0%
CATATAACTATCACTCTCCA+TGG - chr4_4:75303-75322 None:intergenic 35.0%
!! TCAAGAGGTTTTAGTAGAGA+AGG - chr4_4:75614-75633 None:intergenic 35.0%
AGGTTTGTGAGTAATTCAAG+AGG - chr4_4:75629-75648 None:intergenic 35.0%
TGGCAATGTTGTATATCAAG+AGG - chr4_4:75649-75668 None:intergenic 35.0%
TTCACAGAGATTAGGAGTAA+GGG - chr4_4:75691-75710 None:intergenic 35.0%
ATTCACAGAGATTAGGAGTA+AGG - chr4_4:75692-75711 None:intergenic 35.0%
TTTGTTACAGAACTACAGGA+AGG - chr4_4:75866-75885 None:intergenic 35.0%
!! TTGAAATTCTAGGCACTGAT+TGG - chr4_4:75989-76008 None:intergenic 35.0%
!!! CTGTTCAGTTTTGGATATAG+TGG + chr4_4:76012-76031 Msa0662410:intron 35.0%
GATCATGTATAGGACAGTTA+TGG - chr4_4:76101-76120 None:intergenic 35.0%
! TTTGTTGAGGTATGCTTTTG+CGG + chr4_4:76171-76190 Msa0662410:intron 35.0%
TTGAGTAATGTCAAATGAGG+TGG + chr4_4:76381-76400 Msa0662410:intron 35.0%
TTGTATGCAAGTAAGAAGAG+AGG + chr4_4:76628-76647 Msa0662410:CDS 35.0%
AAGAAGAGAGGAAATAGAAG+AGG + chr4_4:76640-76659 Msa0662410:CDS 35.0%
GTGAATACTTAACTGAGACT+TGG + chr4_4:76720-76739 Msa0662410:CDS 35.0%
AGCAAATGAAAAGGGATGAA+GGG - chr4_4:76921-76940 None:intergenic 35.0%
AAGCAAATGAAAAGGGATGA+AGG - chr4_4:76922-76941 None:intergenic 35.0%
AGCATCTTCTCCACTAAAAA+GGG - chr4_4:77036-77055 None:intergenic 35.0%
AAGCATCTTCTCCACTAAAA+AGG - chr4_4:77037-77056 None:intergenic 35.0%
!! AGATGCTTAATGCTTTTTGG+TGG + chr4_4:77049-77068 Msa0662410:intron 35.0%
!! GATGCTTAATGCTTTTTGGT+GGG + chr4_4:77050-77069 Msa0662410:intron 35.0%
!! ATGCTTAATGCTTTTTGGTG+GGG + chr4_4:77051-77070 Msa0662410:intron 35.0%
TTTCGTTGCTAGAATGTTGA+AGG + chr4_4:77227-77246 Msa0662410:intron 35.0%
GAATGATTCAAACTCAACCA+AGG - chr4_4:77299-77318 None:intergenic 35.0%
TCAAATCTTCGTGACATTGT+GGG - chr4_4:77337-77356 None:intergenic 35.0%
ATCAAATCTTCGTGACATTG+TGG - chr4_4:77338-77357 None:intergenic 35.0%
ACAATGTCACGAAGATTTGA+TGG + chr4_4:77337-77356 Msa0662410:intron 35.0%
AGATGTAATAGCTTCAGTCA+TGG - chr4_4:77420-77439 None:intergenic 35.0%
TTTGGAAGATCAAGATGACA+TGG + chr4_4:77453-77472 Msa0662410:intron 35.0%
ATCAAGATGACATGGTGATA+TGG + chr4_4:77461-77480 Msa0662410:intron 35.0%
TGGTAAATACATAGTGAGGT+CGG + chr4_4:77498-77517 Msa0662410:intron 35.0%
GTATTCTTATCTGGCAGATT+TGG + chr4_4:77620-77639 Msa0662410:intron 35.0%
TTGGAGAAAATGCTTACAGA+TGG + chr4_4:77639-77658 Msa0662410:intron 35.0%
TGGAGAAAATGCTTACAGAT+GGG + chr4_4:77640-77659 Msa0662410:intron 35.0%
ACTCAATACTAGTCACACAT+TGG - chr4_4:77751-77770 None:intergenic 35.0%
AAGAAATATGGTCCTACCAT+CGG - chr4_4:78034-78053 None:intergenic 35.0%
!! AAATTCAATTTTGGTCCCTC+GGG - chr4_4:78096-78115 None:intergenic 35.0%
!! AAAAGGCTTGGTTAGAGTTT+TGG - chr4_4:78136-78155 None:intergenic 35.0%
! CTATATTTTGTACAGGCAAG+TGG + chr4_4:78245-78264 Msa0662410:CDS 35.0%
!!! TGTTGCAGTTTTGTTGTAAG+TGG + chr4_4:78478-78497 Msa0662410:three_prime_UTR 35.0%
TTCTTAAGGCTGATAGATGC+AGG + chr4_4:75184-75203 Msa0662410:CDS 40.0%
TCTTAAGGCTGATAGATGCA+GGG + chr4_4:75185-75204 Msa0662410:CDS 40.0%
AATTCAAAACCAGAAGGTGG+AGG - chr4_4:75249-75268 None:intergenic 40.0%
GGTAATTCAAAACCAGAAGG+TGG - chr4_4:75252-75271 None:intergenic 40.0%
CGTGGAAATGATACTCTTGA+AGG - chr4_4:75278-75297 None:intergenic 40.0%
AGTATCATTTCCACGATCCA+TGG + chr4_4:75283-75302 Msa0662410:CDS 40.0%
CTGGTCTGCAATATTCTGTT+TGG + chr4_4:75823-75842 Msa0662410:intron 40.0%
TTTAGCAGACTCTTGATACG+CGG + chr4_4:75960-75979 Msa0662410:intron 40.0%
AGGACAGTTATGGTTATGTG+TGG - chr4_4:76091-76110 None:intergenic 40.0%
TCAATGACACGCAAGATGTT+GGG - chr4_4:76455-76474 None:intergenic 40.0%
ACCTATGTTACTGAACTGCA+CGG + chr4_4:76743-76762 Msa0662410:CDS 40.0%
ATTGGAGCAAAGGAATTCAG+TGG + chr4_4:77082-77101 Msa0662410:intron 40.0%
TTAGTCATGTTAGCCAAGCA+AGG + chr4_4:77177-77196 Msa0662410:intron 40.0%
TAGTCATGTTAGCCAAGCAA+GGG + chr4_4:77178-77197 Msa0662410:intron 40.0%
CTAGAATGTTGAAGGCGAAA+TGG + chr4_4:77235-77254 Msa0662410:intron 40.0%
TAGGGGATGTATAAAGGTGA+AGG + chr4_4:77262-77281 Msa0662410:intron 40.0%
CAAATCTTCGTGACATTGTG+GGG - chr4_4:77336-77355 None:intergenic 40.0%
! ATTTGATGGTTCCTGAAGAG+AGG + chr4_4:77351-77370 Msa0662410:intron 40.0%
GGTAAATACATAGTGAGGTC+GGG + chr4_4:77499-77518 Msa0662410:intron 40.0%
AATGCTTACAGATGGGAGAA+AGG + chr4_4:77647-77666 Msa0662410:intron 40.0%
AATCTTGGTACAACTCACAG+TGG - chr4_4:77700-77719 None:intergenic 40.0%
!! ATTGAGTGCTAGAGTATTGC+AGG + chr4_4:77764-77783 Msa0662410:intron 40.0%
CTGTGTCAAAATTCTGCAGA+CGG - chr4_4:77811-77830 None:intergenic 40.0%
!! CTGCAGAATTTTGACACAGT+TGG + chr4_4:77812-77831 Msa0662410:intron 40.0%
TGTGAAATACAACCGATGGT+AGG + chr4_4:78019-78038 Msa0662410:intron 40.0%
TCGTTCAGCTTATAATCCCT+TGG - chr4_4:78283-78302 None:intergenic 40.0%
TTTATGGATGTCTTTCGCGA+AGG - chr4_4:78318-78337 None:intergenic 40.0%
!!! TTTTAAAGGTGGACTGCTGA+TGG + chr4_4:78379-78398 Msa0662410:three_prime_UTR 40.0%
! AGTCGAAGAAGAGTGCAAAG+TGG + chr4_4:74966-74985 Msa0662410:CDS 45.0%
ATCCTCTTGAGGTCTTGCTT+AGG + chr4_4:75493-75512 Msa0662410:intron 45.0%
! GCTGGCAAGACTATTTTGTG+TGG - chr4_4:75669-75688 None:intergenic 45.0%
TGTCAAATGAGGTGGATGCT+TGG + chr4_4:76389-76408 Msa0662410:intron 45.0%
GTCAATGACACGCAAGATGT+TGG - chr4_4:76456-76475 None:intergenic 45.0%
TGCATACAAGTTTCTGCGTC+TGG - chr4_4:76617-76636 None:intergenic 45.0%
ACCGTGCAGTTCAGTAACAT+AGG - chr4_4:76747-76766 None:intergenic 45.0%
!! CTTAATGCTTTTTGGTGGGG+AGG + chr4_4:77054-77073 Msa0662410:intron 45.0%
GAGCAAAGGAATTCAGTGGT+TGG + chr4_4:77086-77105 Msa0662410:intron 45.0%
!! AGGAATTCAGTGGTTGGCTT+AGG + chr4_4:77092-77111 Msa0662410:intron 45.0%
CCTATCCCTCTCTAAGCTTT+GGG - chr4_4:77132-77151 None:intergenic 45.0%
ACCTATCCCTCTCTAAGCTT+TGG - chr4_4:77133-77152 None:intergenic 45.0%
TAGCATATTCCACCCTTGCT+TGG - chr4_4:77193-77212 None:intergenic 45.0%
GTGGAATATGCTATGCAAGC+TGG + chr4_4:77200-77219 Msa0662410:intron 45.0%
GAATGTTGAAGGCGAAATGG+CGG + chr4_4:77238-77257 Msa0662410:intron 45.0%
ACATTCCATCTCCTCTCTTC+AGG - chr4_4:77365-77384 None:intergenic 45.0%
AGAAGAAACGCTCTGTGCAA+GGG + chr4_4:77554-77573 Msa0662410:intron 45.0%
!! ACAGATTTGCTGAGGGACAT+GGG - chr4_4:77602-77621 None:intergenic 45.0%
!! TACAGATTTGCTGAGGGACA+TGG - chr4_4:77603-77622 None:intergenic 45.0%
TACAGATGGGAGAAAGGTTG+CGG + chr4_4:77653-77672 Msa0662410:intron 45.0%
TGGTACAACTCACAGTGGTT+CGG - chr4_4:77695-77714 None:intergenic 45.0%
AGTTACTCGGAAAAAACCCG+AGG + chr4_4:78077-78096 Msa0662410:intron 45.0%
GTTACTCGGAAAAAACCCGA+GGG + chr4_4:78078-78097 Msa0662410:intron 45.0%
!!! TTTTAAAAATTAAAGAAATA+TGG - chr4_4:78046-78065 None:intergenic 5.0%
!!! TTCTTTAATTTTTAAAATAT+AGG + chr4_4:78049-78068 Msa0662410:intron 5.0%
!!! TCTTTAATTTTTAAAATATA+GGG + chr4_4:78050-78069 Msa0662410:intron 5.0%
! CAAAGTGGAGCATCCACAGA+GGG + chr4_4:74981-75000 Msa0662410:CDS 50.0%
TAAGGCTGATAGATGCAGGG+AGG + chr4_4:75188-75207 Msa0662410:CDS 50.0%
GATGCAGGGAGGCTTACAAA+AGG + chr4_4:75199-75218 Msa0662410:CDS 50.0%
CTATCACTCTCCATGGATCG+TGG - chr4_4:75296-75315 None:intergenic 50.0%
GTCCTAAGCAAGACCTCAAG+AGG - chr4_4:75498-75517 None:intergenic 50.0%
CAGAGATTAGGAGTAAGGGC+TGG - chr4_4:75687-75706 None:intergenic 50.0%
GTTACTGAACTGCACGGTGT+CGG + chr4_4:76749-76768 Msa0662410:CDS 50.0%
!! AATGCTTTTTGGTGGGGAGG+AGG + chr4_4:77057-77076 Msa0662410:intron 50.0%
TTTGGTGGGGAGGAGGTAAT+TGG + chr4_4:77064-77083 Msa0662410:intron 50.0%
GGAGGAGGTAATTGGAGCAA+AGG + chr4_4:77072-77091 Msa0662410:intron 50.0%
TCGTGTCCCAAAGCTTAGAG+AGG + chr4_4:77123-77142 Msa0662410:intron 50.0%
CGTGTCCCAAAGCTTAGAGA+GGG + chr4_4:77124-77143 Msa0662410:intron 50.0%
CCCAAAGCTTAGAGAGGGAT+AGG + chr4_4:77129-77148 Msa0662410:intron 50.0%
TCATGTTAGCCAAGCAAGGG+TGG + chr4_4:77181-77200 Msa0662410:intron 50.0%
!! TTGAAGGCGAAATGGCGGAT+AGG + chr4_4:77243-77262 Msa0662410:intron 50.0%
!! TGAAGGCGAAATGGCGGATA+GGG + chr4_4:77244-77263 Msa0662410:intron 50.0%
GGCGGATAGGGGATGTATAA+AGG + chr4_4:77256-77275 Msa0662410:intron 50.0%
TGAAGGTCATGCAAGACCCT+TGG + chr4_4:77279-77298 Msa0662410:intron 50.0%
TGGTTCCTGAAGAGAGGAGA+TGG + chr4_4:77357-77376 Msa0662410:intron 50.0%
GAGAAGAAACGCTCTGTGCA+AGG + chr4_4:77553-77572 Msa0662410:intron 50.0%
ACAGATGGGAGAAAGGTTGC+GGG + chr4_4:77654-77673 Msa0662410:intron 50.0%
CACAGTTGGAGCTGCGTTAT+TGG + chr4_4:77826-77845 Msa0662410:intron 50.0%
TGTTCAGAGGCAAGCGAACA+TGG + chr4_4:77938-77957 Msa0662410:intron 50.0%
AGTGGGATGAAGTTGTGCCA+AGG + chr4_4:78263-78282 Msa0662410:CDS 50.0%
GTGGGATGAAGTTGTGCCAA+GGG + chr4_4:78264-78283 Msa0662410:CDS 50.0%
!! TAAAGGTGGACTGCTGATGG+TGG + chr4_4:78382-78401 Msa0662410:three_prime_UTR 50.0%
! GCAAAGTGGAGCATCCACAG+AGG + chr4_4:74980-74999 Msa0662410:CDS 55.0%
!! GAAGGCGAAATGGCGGATAG+GGG + chr4_4:77245-77264 Msa0662410:intron 55.0%
GCTCTGTGCAAGGGAAGTGA+CGG + chr4_4:77563-77582 Msa0662410:intron 55.0%
! AAGGGAAGTGACGGAGGTTG+TGG + chr4_4:77572-77591 Msa0662410:intron 55.0%
! GAAGTGACGGAGGTTGTGGA+AGG + chr4_4:77576-77595 Msa0662410:intron 55.0%
!! GACTGCTGATGGTGGAAGGA+GGG + chr4_4:78390-78409 Msa0662410:three_prime_UTR 55.0%
GAACGAGACACGACCCTCTG+TGG - chr4_4:74997-75016 None:intergenic 60.0%
AACTGGGTGCCTCCACCTTC+TGG + chr4_4:75237-75256 Msa0662410:CDS 60.0%
CTGTGCAAGGGAAGTGACGG+AGG + chr4_4:77566-77585 Msa0662410:intron 60.0%
!! GGTGGACTGCTGATGGTGGA+AGG + chr4_4:78386-78405 Msa0662410:three_prime_UTR 60.0%
!! GGACTGCTGATGGTGGAAGG+AGG + chr4_4:78389-78408 Msa0662410:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 74880 78552 74880 ID=Msa0662410;Name=Msa0662410
chr4_4 mRNA 74880 78552 74880 ID=Msa0662410-mRNA-1;Parent=Msa0662410;Name=Msa0662410-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0.5|0.66|1|0.5|1|3|216|245
chr4_4 exon 74880 75308 74880 ID=Msa0662410-mRNA-1:exon:11;Parent=Msa0662410-mRNA-1
chr4_4 exon 76578 76774 76578 ID=Msa0662410-mRNA-1:exon:12;Parent=Msa0662410-mRNA-1
chr4_4 exon 78225 78552 78225 ID=Msa0662410-mRNA-1:exon:13;Parent=Msa0662410-mRNA-1
chr4_4 CDS 74880 75308 74880 ID=Msa0662410-mRNA-1:cds;Parent=Msa0662410-mRNA-1
chr4_4 CDS 76578 76774 76578 ID=Msa0662410-mRNA-1:cds;Parent=Msa0662410-mRNA-1
chr4_4 CDS 78225 78336 78225 ID=Msa0662410-mRNA-1:cds;Parent=Msa0662410-mRNA-1
chr4_4 three_prime_UTR 78337 78552 78337 ID=Msa0662410-mRNA-1:three_prime_utr;Parent=Msa0662410-mRNA-1
Gene Sequence

>Msa0662410

AAGTATGTGTATAAGCGAAATCATCTTACAAAGAGTGAAGTTGAACAAGAGTGTAAAGTTAGGGTCGTGTCTCGTTATTTTCATAAAGTCGAAGAAGAGTGCAAAGTGGAGCATCCACAGAGGGTCGTGTCTCGTTCTACGAAGAGCATTGAAGATTTAGAACAGTTTAGGTATCAGAGTGAAAAAGTGTCAAGTCTAAGAGAGTGTGTTAAGAACAGTGAACTTCCTCCTAAGATTTTCAATGACTCTCGAAAGAGACCGAAAGTTGAAAAGCCTATGAAATCCAAAAGAAAAGCTAAACCTTTTCTTAAGGCTGATAGATGCAGGGAGGCTTACAAAAGGAAAACACTTGATAATAACTGGGTGCCTCCACCTTCTGGTTTTGAATTACCTCTCCTTCAAGAGTATCATTTCCACGATCCATGGAGAGCAGAACAAGTTTTAGATAACTTTTTTGAGTTATGTCCAGACGCAGAAACTTGTATGCAAGTAAGAAGAGAGGAAATAGAAGAGGTAATAAAGACATTAGGTTTTCAAGTGAAAAGATCAAGAAGTTTGCAACGCTTTTCTCGTGAATACTTAACTGAGACTTGGACCTATGTTACTGAACTGCACGGTGTCGGCAAGTATGCTGCGGATGCATATGCTATATTTTGTACAGGCAAGTGGGATGAAGTTGTGCCAAGGGATTATAAGCTGAACGAATATTGGAATTTCCTTCGCGAAAGACATCCATAA
Protein sequence

>Msa0662410

KYVYKRNHLTKSEVEQECKVRVVSRYFHKVEEECKVEHPQRVVSRSTKSIEDLEQFRYQSEKVSSLRECVKNSELPPKIFNDSRKRPKVEKPMKSKRKAKPFLKADRCREAYKRKTLDNNWVPPPSGFELPLLQEYHFHDPWRAEQVLDNFFELCPDAETCMQVRREEIEEVIKTLGFQVKRSRSLQRFSREYLTETWTYVTELHGVGKYAADAYAIFCTGKWDEVVPRDYKLNEYWNFLRERHP*