AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1376630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376630 MtrunA17_Chr3g0145791 96.373 193 7 0 1 193 1 193 4.25e-135 375
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376630 AT5G38110.1 86.391 169 22 1 1 168 1 169 1.18e-104 300
Msa1376630 AT1G66740.1 80.899 178 31 1 1 178 1 175 2.43e-103 296

Find 62 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTACTCACATGCTCTTATT+TGG 0.139177 tig0019825:-119619 Msa1376630:CDS
TGGATCTTGTTCTCCAGTTT+GGG 0.231870 tig0019825:+119392 None:intergenic
GTGGATCTTGTTCTCCAGTT+TGG 0.266545 tig0019825:+119391 None:intergenic
AATGCAAGATCCAGCGAAAT+TGG 0.267575 tig0019825:+119151 None:intergenic
CTACTCACATGCTCTTATTT+GGG 0.286175 tig0019825:-119618 Msa1376630:CDS
GATATCTTCTTCACGAATCT+TGG 0.287365 tig0019825:+119659 None:intergenic
TGAGACTTATGACCAGTTAT+TGG 0.289525 tig0019825:-119754 Msa1376630:CDS
CGAGATTTCCTATGAGTGTT+TGG 0.353611 tig0019825:-120112 Msa1376630:CDS
TGAAAAGAGAATAAAGATTA+AGG 0.358393 tig0019825:+119010 None:intergenic
AACGAATTGTGGATAGTAAC+TGG 0.370258 tig0019825:+119185 None:intergenic
GGGTAGTTGCTCTGAGGGAG+GGG 0.371206 tig0019825:+119413 None:intergenic
TGGGGTAGTTGCTCTGAGGG+AGG 0.378053 tig0019825:+119411 None:intergenic
GGGGTAGTTGCTCTGAGGGA+GGG 0.392562 tig0019825:+119412 None:intergenic
GTTCTCAGGGTGGAAATTGA+TGG 0.403676 tig0019825:+119455 None:intergenic
GGTAGTTGCTCTGAGGGAGG+GGG 0.407080 tig0019825:+119414 None:intergenic
CAAGGACACTCTCCAATAAC+TGG 0.417188 tig0019825:+119742 None:intergenic
TCACGAATCTTGGATGGGTC+TGG 0.425679 tig0019825:+119669 None:intergenic
TCAGAGCAACTACCCCAAAC+TGG 0.436456 tig0019825:-119405 Msa1376630:CDS
GAGAGCGGCCAAACACTCAT+AGG 0.445137 tig0019825:+120104 None:intergenic
CTATCTGTTAAAACCTTGGT+TGG 0.449270 tig0019825:+119525 None:intergenic
AACCCTATCTGTTAAAACCT+TGG 0.452925 tig0019825:+119521 None:intergenic
TTATTGGAGAGTGTCCTTGT+TGG 0.475012 tig0019825:-119738 Msa1376630:CDS
TTCTCAGGGTGGAAATTGAT+GGG 0.479943 tig0019825:+119456 None:intergenic
CGATACAAATCCAATTTCGC+TGG 0.481461 tig0019825:-119161 Msa1376630:three_prime_UTR
TCTGTTAAAACCTTGGTTGG+AGG 0.483580 tig0019825:+119528 None:intergenic
GAATGGAAGCTGATTTATGT+TGG 0.485287 tig0019825:-119789 Msa1376630:CDS
TCTTCTTCACGAATCTTGGA+TGG 0.488809 tig0019825:+119663 None:intergenic
ATGGGGAACTTAGTGACCCT+TGG 0.490528 tig0019825:+119474 None:intergenic
GAGAGGGCAATGAAATGTAT+TGG 0.492460 tig0019825:+120298 None:intergenic
GGTCAAGAGTTTATTCGTGT+TGG 0.494710 tig0019825:-119597 Msa1376630:CDS
CTTGTTGGTCCTGTCAATGT+TGG 0.502550 tig0019825:-119723 Msa1376630:CDS
CGATAGTTTCCAACATTGAC+AGG 0.504203 tig0019825:+119714 None:intergenic
ATTTGTATCGCAACGAATTG+TGG 0.504640 tig0019825:+119174 None:intergenic
TAGGAAATCTCGAACTGAAA+GGG 0.513564 tig0019825:+120123 None:intergenic
AAGGGATTGAGAAACGAAGC+TGG 0.517879 tig0019825:+120141 None:intergenic
GTTTGGGGTAGTTGCTCTGA+GGG 0.518854 tig0019825:+119408 None:intergenic
GTAGAAATAACTCGGGCAAA+CGG 0.521989 tig0019825:+119080 None:intergenic
GAGAGAGGAACCTCCAACCA+AGG 0.524167 tig0019825:-119538 Msa1376630:CDS
ATTCGTGAAGAAGATATCAT+TGG 0.528304 tig0019825:-119654 Msa1376630:CDS
CAATTGTCACAGATTTGGAA+TGG 0.533603 tig0019825:-119806 Msa1376630:intron
ATAGGAAATCTCGAACTGAA+AGG 0.536457 tig0019825:+120122 None:intergenic
CGAATCTTGGATGGGTCTGG+TGG 0.541608 tig0019825:+119672 None:intergenic
TGTTTGGCCGCTCTCAAAGA+TGG 0.557561 tig0019825:-120096 Msa1376630:intron
CTTCTTCACGAATCTTGGAT+GGG 0.564124 tig0019825:+119664 None:intergenic
GGATCTTGTTCTCCAGTTTG+GGG 0.567661 tig0019825:+119393 None:intergenic
GCTAGAAAGTAGAAATAACT+CGG 0.577591 tig0019825:+119072 None:intergenic
AGTTTGGGGTAGTTGCTCTG+AGG 0.582073 tig0019825:+119407 None:intergenic
GTATCGGGTGCAGCAGCAAG+TGG 0.584533 tig0019825:+119372 None:intergenic
AGGGCAATGAAATGTATTGG+CGG 0.594879 tig0019825:+120301 None:intergenic
ACGAAAATGAAAATAAGAGA+GGG 0.613145 tig0019825:+120282 None:intergenic
CTAGAAAGTAGAAATAACTC+GGG 0.618457 tig0019825:+119073 None:intergenic
CAACATTGACAGGACCAACA+AGG 0.622504 tig0019825:+119724 None:intergenic
TGACGACGAGCAGCTGAGAG+AGG 0.624627 tig0019825:-119553 Msa1376630:CDS
CACGAAAATGAAAATAAGAG+AGG 0.626674 tig0019825:+120281 None:intergenic
ACATCTTATCTGATAAACCA+AGG 0.636524 tig0019825:-119491 Msa1376630:CDS
GATTTATGTTGGATCTGCTG+AGG 0.636648 tig0019825:-119778 Msa1376630:CDS
GAAACGAAGCTGGATTGTCA+AGG 0.636849 tig0019825:+120151 None:intergenic
AAGAAGAAAAGCGATGAGCG+CGG 0.647668 tig0019825:-120196 Msa1376630:exon
CATCTTATCTGATAAACCAA+GGG 0.651929 tig0019825:-119490 Msa1376630:CDS
GGTTAACATTACGAACGTGA+CGG 0.673152 tig0019825:-120175 Msa1376630:CDS
TCTCAGGGTGGAAATTGATG+GGG 0.674973 tig0019825:+119457 None:intergenic
ATACATACCATCTTTGAGAG+CGG 0.695677 tig0019825:+120089 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTCAAATCAAAAAGA+GGG + tig0019825:119052-119071 None:intergenic 15.0%
!! TATTTATTCAAATCAAAAAG+AGG + tig0019825:119053-119072 None:intergenic 15.0%
!!! TTTACTCTACTTTTATTTTA+GGG - tig0019825:119243-119262 Msa1376630:three_prime_UTR 15.0%
!! ATTTATTCAAATCAAAAAGA+GGG + tig0019825:119052-119071 None:intergenic 15.0%
!! TATTTATTCAAATCAAAAAG+AGG + tig0019825:119053-119072 None:intergenic 15.0%
!!! TTTACTCTACTTTTATTTTA+GGG - tig0019825:119243-119262 Msa1376630:three_prime_UTR 15.0%
!!! CTTTACTCTACTTTTATTTT+AGG - tig0019825:119242-119261 Msa1376630:three_prime_UTR 20.0%
!! AACAAAATTAGGTCAAATTT+GGG + tig0019825:119349-119368 None:intergenic 20.0%
!! AAACAAAATTAGGTCAAATT+TGG + tig0019825:119350-119369 None:intergenic 20.0%
!! ACTAAGTAACAAACAAAATT+AGG + tig0019825:119360-119379 None:intergenic 20.0%
!!! TCTACTTTCTTGTTTTTTTA+GGG - tig0019825:119398-119417 Msa1376630:CDS 20.0%
!! TTCAACTTAATAATTGCATT+GGG + tig0019825:119448-119467 None:intergenic 20.0%
!! TTTCAACTTAATAATTGCAT+TGG + tig0019825:119449-119468 None:intergenic 20.0%
!!! CAATTATTAAGTTGAAAATG+TGG - tig0019825:119452-119471 Msa1376630:CDS 20.0%
!! TGAAAAGAGAATAAAGATTA+AGG + tig0019825:120294-120313 None:intergenic 20.0%
!!! CTTTACTCTACTTTTATTTT+AGG - tig0019825:119242-119261 Msa1376630:three_prime_UTR 20.0%
!! AACAAAATTAGGTCAAATTT+GGG + tig0019825:119349-119368 None:intergenic 20.0%
!! AAACAAAATTAGGTCAAATT+TGG + tig0019825:119350-119369 None:intergenic 20.0%
!! ACTAAGTAACAAACAAAATT+AGG + tig0019825:119360-119379 None:intergenic 20.0%
!!! TCTACTTTCTTGTTTTTTTA+GGG - tig0019825:119398-119417 Msa1376630:CDS 20.0%
!! TTCAACTTAATAATTGCATT+GGG + tig0019825:119448-119467 None:intergenic 20.0%
!! TTTCAACTTAATAATTGCAT+TGG + tig0019825:119449-119468 None:intergenic 20.0%
!!! CAATTATTAAGTTGAAAATG+TGG - tig0019825:119452-119471 Msa1376630:CDS 20.0%
!! TGAAAAGAGAATAAAGATTA+AGG + tig0019825:120294-120313 None:intergenic 20.0%
! ACGAAAATGAAAATAAGAGA+GGG + tig0019825:119022-119041 None:intergenic 25.0%
!! TGTTTGTTACTTAGTTAGTT+AGG - tig0019825:119365-119384 Msa1376630:CDS 25.0%
!! GTTTGTTACTTAGTTAGTTA+GGG - tig0019825:119366-119385 Msa1376630:CDS 25.0%
! CTAAAAAAACAAGAAAGTAG+AGG + tig0019825:119399-119418 None:intergenic 25.0%
!!! CTCTACTTTCTTGTTTTTTT+AGG - tig0019825:119397-119416 Msa1376630:CDS 25.0%
!!! TTCTTGTTTTTTTAGGGTTT+AGG - tig0019825:119404-119423 Msa1376630:CDS 25.0%
! TCAACTTAATAATTGCATTG+GGG + tig0019825:119447-119466 None:intergenic 25.0%
!!! TACTTTTTGTCAATCTCTTA+TGG - tig0019825:120032-120051 Msa1376630:intron 25.0%
! ACGAAAATGAAAATAAGAGA+GGG + tig0019825:119022-119041 None:intergenic 25.0%
!! TGTTTGTTACTTAGTTAGTT+AGG - tig0019825:119365-119384 Msa1376630:CDS 25.0%
!! GTTTGTTACTTAGTTAGTTA+GGG - tig0019825:119366-119385 Msa1376630:CDS 25.0%
! CTAAAAAAACAAGAAAGTAG+AGG + tig0019825:119399-119418 None:intergenic 25.0%
!!! CTCTACTTTCTTGTTTTTTT+AGG - tig0019825:119397-119416 Msa1376630:CDS 25.0%
!!! TTCTTGTTTTTTTAGGGTTT+AGG - tig0019825:119404-119423 Msa1376630:CDS 25.0%
! TCAACTTAATAATTGCATTG+GGG + tig0019825:119447-119466 None:intergenic 25.0%
!!! TACTTTTTGTCAATCTCTTA+TGG - tig0019825:120032-120051 Msa1376630:intron 25.0%
CACGAAAATGAAAATAAGAG+AGG + tig0019825:119023-119042 None:intergenic 30.0%
TCAAATCAAAAAGAGGGAAA+GGG + tig0019825:119046-119065 None:intergenic 30.0%
TTCAAATCAAAAAGAGGGAA+AGG + tig0019825:119047-119066 None:intergenic 30.0%
AAAACAAGAAAGTAGAGGAT+TGG + tig0019825:119394-119413 None:intergenic 30.0%
AACTTAATAATTGCATTGGG+GGG + tig0019825:119445-119464 None:intergenic 30.0%
CAACTTAATAATTGCATTGG+GGG + tig0019825:119446-119465 None:intergenic 30.0%
!!! AAACTATCGCTTTGTTTTAC+AGG - tig0019825:119601-119620 Msa1376630:CDS 30.0%
ATTCGTGAAGAAGATATCAT+TGG - tig0019825:119647-119666 Msa1376630:CDS 30.0%
!! AACCAAGGTTTTAACAGATA+GGG - tig0019825:119778-119797 Msa1376630:CDS 30.0%
! TTTTAACAGATAGGGTTCAA+AGG - tig0019825:119786-119805 Msa1376630:CDS 30.0%
ACATCTTATCTGATAAACCA+AGG - tig0019825:119810-119829 Msa1376630:intron 30.0%
CATCTTATCTGATAAACCAA+GGG - tig0019825:119811-119830 Msa1376630:intron 30.0%
! CTTCATTTTCATTGTTCTCA+GGG + tig0019825:119862-119881 None:intergenic 30.0%
! TCTTCATTTTCATTGTTCTC+AGG + tig0019825:119863-119882 None:intergenic 30.0%
! AGGCTTTTCAATTACATGTA+TGG - tig0019825:120002-120021 Msa1376630:intron 30.0%
CTAGAAAGTAGAAATAACTC+GGG + tig0019825:120231-120250 None:intergenic 30.0%
GCTAGAAAGTAGAAATAACT+CGG + tig0019825:120232-120251 None:intergenic 30.0%
CACGAAAATGAAAATAAGAG+AGG + tig0019825:119023-119042 None:intergenic 30.0%
TCAAATCAAAAAGAGGGAAA+GGG + tig0019825:119046-119065 None:intergenic 30.0%
TTCAAATCAAAAAGAGGGAA+AGG + tig0019825:119047-119066 None:intergenic 30.0%
AAAACAAGAAAGTAGAGGAT+TGG + tig0019825:119394-119413 None:intergenic 30.0%
AACTTAATAATTGCATTGGG+GGG + tig0019825:119445-119464 None:intergenic 30.0%
CAACTTAATAATTGCATTGG+GGG + tig0019825:119446-119465 None:intergenic 30.0%
!!! AAACTATCGCTTTGTTTTAC+AGG - tig0019825:119601-119620 Msa1376630:CDS 30.0%
ATTCGTGAAGAAGATATCAT+TGG - tig0019825:119647-119666 Msa1376630:CDS 30.0%
!! AACCAAGGTTTTAACAGATA+GGG - tig0019825:119778-119797 Msa1376630:CDS 30.0%
! TTTTAACAGATAGGGTTCAA+AGG - tig0019825:119786-119805 Msa1376630:CDS 30.0%
ACATCTTATCTGATAAACCA+AGG - tig0019825:119810-119829 Msa1376630:intron 30.0%
CATCTTATCTGATAAACCAA+GGG - tig0019825:119811-119830 Msa1376630:intron 30.0%
! CTTCATTTTCATTGTTCTCA+GGG + tig0019825:119862-119881 None:intergenic 30.0%
! TCTTCATTTTCATTGTTCTC+AGG + tig0019825:119863-119882 None:intergenic 30.0%
! AGGCTTTTCAATTACATGTA+TGG - tig0019825:120002-120021 Msa1376630:intron 30.0%
CTAGAAAGTAGAAATAACTC+GGG + tig0019825:120231-120250 None:intergenic 30.0%
GCTAGAAAGTAGAAATAACT+CGG + tig0019825:120232-120251 None:intergenic 30.0%
TAGGAAATCTCGAACTGAAA+GGG + tig0019825:119181-119200 None:intergenic 35.0%
ATAGGAAATCTCGAACTGAA+AGG + tig0019825:119182-119201 None:intergenic 35.0%
! ATACATACCATCTTTGAGAG+CGG + tig0019825:119215-119234 None:intergenic 35.0%
! ATTTTCGTTTTCACTCTGCT+AGG - tig0019825:119283-119302 Msa1376630:three_prime_UTR 35.0%
! GTGGACTATCAATTCATTCA+TGG - tig0019825:119471-119490 Msa1376630:CDS 35.0%
ATGGTCAATTGTCACAGATT+TGG - tig0019825:119490-119509 Msa1376630:CDS 35.0%
CAATTGTCACAGATTTGGAA+TGG - tig0019825:119495-119514 Msa1376630:CDS 35.0%
! GAATGGAAGCTGATTTATGT+TGG - tig0019825:119512-119531 Msa1376630:CDS 35.0%
TGAGACTTATGACCAGTTAT+TGG - tig0019825:119547-119566 Msa1376630:CDS 35.0%
GATATCTTCTTCACGAATCT+TGG + tig0019825:119645-119664 None:intergenic 35.0%
TCTACTCACATGCTCTTATT+TGG - tig0019825:119682-119701 Msa1376630:CDS 35.0%
! CTACTCACATGCTCTTATTT+GGG - tig0019825:119683-119702 Msa1376630:CDS 35.0%
CTATCTGTTAAAACCTTGGT+TGG + tig0019825:119779-119798 None:intergenic 35.0%
!! CAACCAAGGTTTTAACAGAT+AGG - tig0019825:119777-119796 Msa1376630:CDS 35.0%
AACCCTATCTGTTAAAACCT+TGG + tig0019825:119783-119802 None:intergenic 35.0%
!!! TTTTGTTGGTGTTGCTATAC+AGG - tig0019825:119982-120001 Msa1376630:intron 35.0%
AACGAATTGTGGATAGTAAC+TGG + tig0019825:120119-120138 None:intergenic 35.0%
ATTTGTATCGCAACGAATTG+TGG + tig0019825:120130-120149 None:intergenic 35.0%
TAGGAAATCTCGAACTGAAA+GGG + tig0019825:119181-119200 None:intergenic 35.0%
ATAGGAAATCTCGAACTGAA+AGG + tig0019825:119182-119201 None:intergenic 35.0%
! ATACATACCATCTTTGAGAG+CGG + tig0019825:119215-119234 None:intergenic 35.0%
! ATTTTCGTTTTCACTCTGCT+AGG - tig0019825:119283-119302 Msa1376630:three_prime_UTR 35.0%
! GTGGACTATCAATTCATTCA+TGG - tig0019825:119471-119490 Msa1376630:CDS 35.0%
ATGGTCAATTGTCACAGATT+TGG - tig0019825:119490-119509 Msa1376630:CDS 35.0%
CAATTGTCACAGATTTGGAA+TGG - tig0019825:119495-119514 Msa1376630:CDS 35.0%
! GAATGGAAGCTGATTTATGT+TGG - tig0019825:119512-119531 Msa1376630:CDS 35.0%
TGAGACTTATGACCAGTTAT+TGG - tig0019825:119547-119566 Msa1376630:CDS 35.0%
GATATCTTCTTCACGAATCT+TGG + tig0019825:119645-119664 None:intergenic 35.0%
TCTACTCACATGCTCTTATT+TGG - tig0019825:119682-119701 Msa1376630:CDS 35.0%
! CTACTCACATGCTCTTATTT+GGG - tig0019825:119683-119702 Msa1376630:CDS 35.0%
CTATCTGTTAAAACCTTGGT+TGG + tig0019825:119779-119798 None:intergenic 35.0%
!! CAACCAAGGTTTTAACAGAT+AGG - tig0019825:119777-119796 Msa1376630:CDS 35.0%
AACCCTATCTGTTAAAACCT+TGG + tig0019825:119783-119802 None:intergenic 35.0%
!!! TTTTGTTGGTGTTGCTATAC+AGG - tig0019825:119982-120001 Msa1376630:intron 35.0%
AACGAATTGTGGATAGTAAC+TGG + tig0019825:120119-120138 None:intergenic 35.0%
ATTTGTATCGCAACGAATTG+TGG + tig0019825:120130-120149 None:intergenic 35.0%
GAGAGGGCAATGAAATGTAT+TGG + tig0019825:119006-119025 None:intergenic 40.0%
GGTTAACATTACGAACGTGA+CGG - tig0019825:119126-119145 Msa1376630:three_prime_UTR 40.0%
CGAGATTTCCTATGAGTGTT+TGG - tig0019825:119189-119208 Msa1376630:three_prime_UTR 40.0%
! GTTTTCACTCTGCTAGGTTA+GGG - tig0019825:119289-119308 Msa1376630:three_prime_UTR 40.0%
TAATAATTGCATTGGGGGGA+GGG + tig0019825:119441-119460 None:intergenic 40.0%
TTAATAATTGCATTGGGGGG+AGG + tig0019825:119442-119461 None:intergenic 40.0%
GATTTATGTTGGATCTGCTG+AGG - tig0019825:119523-119542 Msa1376630:CDS 40.0%
!! TTATTGGAGAGTGTCCTTGT+TGG - tig0019825:119563-119582 Msa1376630:CDS 40.0%
CGATAGTTTCCAACATTGAC+AGG + tig0019825:119590-119609 None:intergenic 40.0%
CTTCTTCACGAATCTTGGAT+GGG + tig0019825:119640-119659 None:intergenic 40.0%
TCTTCTTCACGAATCTTGGA+TGG + tig0019825:119641-119660 None:intergenic 40.0%
GGTCAAGAGTTTATTCGTGT+TGG - tig0019825:119704-119723 Msa1376630:CDS 40.0%
TCTGTTAAAACCTTGGTTGG+AGG + tig0019825:119776-119795 None:intergenic 40.0%
TTCTCAGGGTGGAAATTGAT+GGG + tig0019825:119848-119867 None:intergenic 40.0%
! CATTTTCATTGTTCTCAGGG+TGG + tig0019825:119859-119878 None:intergenic 40.0%
TGGATCTTGTTCTCCAGTTT+GGG + tig0019825:119912-119931 None:intergenic 40.0%
! GGGGGTTCTTAACATTTTGT+TGG - tig0019825:119968-119987 Msa1376630:intron 40.0%
CGATACAAATCCAATTTCGC+TGG - tig0019825:120140-120159 Msa1376630:CDS 40.0%
AATGCAAGATCCAGCGAAAT+TGG + tig0019825:120153-120172 None:intergenic 40.0%
GTAGAAATAACTCGGGCAAA+CGG + tig0019825:120224-120243 None:intergenic 40.0%
GAGAGGGCAATGAAATGTAT+TGG + tig0019825:119006-119025 None:intergenic 40.0%
GGTTAACATTACGAACGTGA+CGG - tig0019825:119126-119145 Msa1376630:three_prime_UTR 40.0%
CGAGATTTCCTATGAGTGTT+TGG - tig0019825:119189-119208 Msa1376630:three_prime_UTR 40.0%
! GTTTTCACTCTGCTAGGTTA+GGG - tig0019825:119289-119308 Msa1376630:three_prime_UTR 40.0%
TAATAATTGCATTGGGGGGA+GGG + tig0019825:119441-119460 None:intergenic 40.0%
TTAATAATTGCATTGGGGGG+AGG + tig0019825:119442-119461 None:intergenic 40.0%
GATTTATGTTGGATCTGCTG+AGG - tig0019825:119523-119542 Msa1376630:CDS 40.0%
!! TTATTGGAGAGTGTCCTTGT+TGG - tig0019825:119563-119582 Msa1376630:CDS 40.0%
CGATAGTTTCCAACATTGAC+AGG + tig0019825:119590-119609 None:intergenic 40.0%
CTTCTTCACGAATCTTGGAT+GGG + tig0019825:119640-119659 None:intergenic 40.0%
TCTTCTTCACGAATCTTGGA+TGG + tig0019825:119641-119660 None:intergenic 40.0%
GGTCAAGAGTTTATTCGTGT+TGG - tig0019825:119704-119723 Msa1376630:CDS 40.0%
TCTGTTAAAACCTTGGTTGG+AGG + tig0019825:119776-119795 None:intergenic 40.0%
TTCTCAGGGTGGAAATTGAT+GGG + tig0019825:119848-119867 None:intergenic 40.0%
! CATTTTCATTGTTCTCAGGG+TGG + tig0019825:119859-119878 None:intergenic 40.0%
TGGATCTTGTTCTCCAGTTT+GGG + tig0019825:119912-119931 None:intergenic 40.0%
! GGGGGTTCTTAACATTTTGT+TGG - tig0019825:119968-119987 Msa1376630:intron 40.0%
CGATACAAATCCAATTTCGC+TGG - tig0019825:120140-120159 Msa1376630:CDS 40.0%
AATGCAAGATCCAGCGAAAT+TGG + tig0019825:120153-120172 None:intergenic 40.0%
GTAGAAATAACTCGGGCAAA+CGG + tig0019825:120224-120243 None:intergenic 40.0%
AAGAAGAAAAGCGATGAGCG+CGG - tig0019825:119105-119124 Msa1376630:three_prime_UTR 45.0%
GAAACGAAGCTGGATTGTCA+AGG + tig0019825:119153-119172 None:intergenic 45.0%
AAGGGATTGAGAAACGAAGC+TGG + tig0019825:119163-119182 None:intergenic 45.0%
! CGTTTTCACTCTGCTAGGTT+AGG - tig0019825:119288-119307 Msa1376630:three_prime_UTR 45.0%
ACTCTGCTAGGTTAGGGTTT+AGG - tig0019825:119295-119314 Msa1376630:three_prime_UTR 45.0%
CTCTGCTAGGTTAGGGTTTA+GGG - tig0019825:119296-119315 Msa1376630:three_prime_UTR 45.0%
AATAATTGCATTGGGGGGAG+GGG + tig0019825:119440-119459 None:intergenic 45.0%
CAAGGACACTCTCCAATAAC+TGG + tig0019825:119562-119581 None:intergenic 45.0%
CAACATTGACAGGACCAACA+AGG + tig0019825:119580-119599 None:intergenic 45.0%
CTTGTTGGTCCTGTCAATGT+TGG - tig0019825:119578-119597 Msa1376630:CDS 45.0%
TCTCAGGGTGGAAATTGATG+GGG + tig0019825:119847-119866 None:intergenic 45.0%
GTTCTCAGGGTGGAAATTGA+TGG + tig0019825:119849-119868 None:intergenic 45.0%
GGATCTTGTTCTCCAGTTTG+GGG + tig0019825:119911-119930 None:intergenic 45.0%
GTGGATCTTGTTCTCCAGTT+TGG + tig0019825:119913-119932 None:intergenic 45.0%
TCATTTGGGGGAATGGTATC+GGG + tig0019825:119947-119966 None:intergenic 45.0%
CTCATTTGGGGGAATGGTAT+CGG + tig0019825:119948-119967 None:intergenic 45.0%
GATACCATTCCCCCAAATGA+GGG - tig0019825:119947-119966 Msa1376630:intron 45.0%
ATACCATTCCCCCAAATGAG+GGG - tig0019825:119948-119967 Msa1376630:intron 45.0%
TTAAGAACCCCCCTCATTTG+GGG + tig0019825:119960-119979 None:intergenic 45.0%
GTTAAGAACCCCCCTCATTT+GGG + tig0019825:119961-119980 None:intergenic 45.0%
TGTTAAGAACCCCCCTCATT+TGG + tig0019825:119962-119981 None:intergenic 45.0%
AAGAAGAAAAGCGATGAGCG+CGG - tig0019825:119105-119124 Msa1376630:three_prime_UTR 45.0%
GAAACGAAGCTGGATTGTCA+AGG + tig0019825:119153-119172 None:intergenic 45.0%
AAGGGATTGAGAAACGAAGC+TGG + tig0019825:119163-119182 None:intergenic 45.0%
! CGTTTTCACTCTGCTAGGTT+AGG - tig0019825:119288-119307 Msa1376630:three_prime_UTR 45.0%
ACTCTGCTAGGTTAGGGTTT+AGG - tig0019825:119295-119314 Msa1376630:three_prime_UTR 45.0%
CTCTGCTAGGTTAGGGTTTA+GGG - tig0019825:119296-119315 Msa1376630:three_prime_UTR 45.0%
AATAATTGCATTGGGGGGAG+GGG + tig0019825:119440-119459 None:intergenic 45.0%
CAAGGACACTCTCCAATAAC+TGG + tig0019825:119562-119581 None:intergenic 45.0%
CAACATTGACAGGACCAACA+AGG + tig0019825:119580-119599 None:intergenic 45.0%
CTTGTTGGTCCTGTCAATGT+TGG - tig0019825:119578-119597 Msa1376630:CDS 45.0%
TCTCAGGGTGGAAATTGATG+GGG + tig0019825:119847-119866 None:intergenic 45.0%
GTTCTCAGGGTGGAAATTGA+TGG + tig0019825:119849-119868 None:intergenic 45.0%
GGATCTTGTTCTCCAGTTTG+GGG + tig0019825:119911-119930 None:intergenic 45.0%
GTGGATCTTGTTCTCCAGTT+TGG + tig0019825:119913-119932 None:intergenic 45.0%
TCATTTGGGGGAATGGTATC+GGG + tig0019825:119947-119966 None:intergenic 45.0%
CTCATTTGGGGGAATGGTAT+CGG + tig0019825:119948-119967 None:intergenic 45.0%
GATACCATTCCCCCAAATGA+GGG - tig0019825:119947-119966 Msa1376630:intron 45.0%
ATACCATTCCCCCAAATGAG+GGG - tig0019825:119948-119967 Msa1376630:intron 45.0%
TTAAGAACCCCCCTCATTTG+GGG + tig0019825:119960-119979 None:intergenic 45.0%
GTTAAGAACCCCCCTCATTT+GGG + tig0019825:119961-119980 None:intergenic 45.0%
TGTTAAGAACCCCCCTCATT+TGG + tig0019825:119962-119981 None:intergenic 45.0%
TGTTTGGCCGCTCTCAAAGA+TGG - tig0019825:119205-119224 Msa1376630:three_prime_UTR 50.0%
TCACGAATCTTGGATGGGTC+TGG + tig0019825:119635-119654 None:intergenic 50.0%
ATGGGGAACTTAGTGACCCT+TGG + tig0019825:119830-119849 None:intergenic 50.0%
! GTTTGGGGTAGTTGCTCTGA+GGG + tig0019825:119896-119915 None:intergenic 50.0%
! AGTTTGGGGTAGTTGCTCTG+AGG + tig0019825:119897-119916 None:intergenic 50.0%
TCAGAGCAACTACCCCAAAC+TGG - tig0019825:119896-119915 Msa1376630:intron 50.0%
CGATACCATTCCCCCAAATG+AGG - tig0019825:119946-119965 Msa1376630:intron 50.0%
TACCATTCCCCCAAATGAGG+GGG - tig0019825:119949-119968 Msa1376630:intron 50.0%
TAAGAACCCCCCTCATTTGG+GGG + tig0019825:119959-119978 None:intergenic 50.0%
TGTTTGGCCGCTCTCAAAGA+TGG - tig0019825:119205-119224 Msa1376630:three_prime_UTR 50.0%
TCACGAATCTTGGATGGGTC+TGG + tig0019825:119635-119654 None:intergenic 50.0%
ATGGGGAACTTAGTGACCCT+TGG + tig0019825:119830-119849 None:intergenic 50.0%
! GTTTGGGGTAGTTGCTCTGA+GGG + tig0019825:119896-119915 None:intergenic 50.0%
! AGTTTGGGGTAGTTGCTCTG+AGG + tig0019825:119897-119916 None:intergenic 50.0%
TCAGAGCAACTACCCCAAAC+TGG - tig0019825:119896-119915 Msa1376630:intron 50.0%
CGATACCATTCCCCCAAATG+AGG - tig0019825:119946-119965 Msa1376630:intron 50.0%
TACCATTCCCCCAAATGAGG+GGG - tig0019825:119949-119968 Msa1376630:intron 50.0%
TAAGAACCCCCCTCATTTGG+GGG + tig0019825:119959-119978 None:intergenic 50.0%
GAGAGCGGCCAAACACTCAT+AGG + tig0019825:119200-119219 None:intergenic 55.0%
CGAATCTTGGATGGGTCTGG+TGG + tig0019825:119632-119651 None:intergenic 55.0%
GAGAGAGGAACCTCCAACCA+AGG - tig0019825:119763-119782 Msa1376630:CDS 55.0%
ACCATTCCCCCAAATGAGGG+GGG - tig0019825:119950-119969 Msa1376630:intron 55.0%
GAGAGCGGCCAAACACTCAT+AGG + tig0019825:119200-119219 None:intergenic 55.0%
CGAATCTTGGATGGGTCTGG+TGG + tig0019825:119632-119651 None:intergenic 55.0%
GAGAGAGGAACCTCCAACCA+AGG - tig0019825:119763-119782 Msa1376630:CDS 55.0%
ACCATTCCCCCAAATGAGGG+GGG - tig0019825:119950-119969 Msa1376630:intron 55.0%
TGACGACGAGCAGCTGAGAG+AGG - tig0019825:119748-119767 Msa1376630:CDS 60.0%
!! GGTAGTTGCTCTGAGGGAGG+GGG + tig0019825:119890-119909 None:intergenic 60.0%
!! GGGTAGTTGCTCTGAGGGAG+GGG + tig0019825:119891-119910 None:intergenic 60.0%
!! GGGGTAGTTGCTCTGAGGGA+GGG + tig0019825:119892-119911 None:intergenic 60.0%
!! TGGGGTAGTTGCTCTGAGGG+AGG + tig0019825:119893-119912 None:intergenic 60.0%
GTATCGGGTGCAGCAGCAAG+TGG + tig0019825:119932-119951 None:intergenic 60.0%
ACCCCCCTCATTTGGGGGAA+TGG + tig0019825:119954-119973 None:intergenic 60.0%
TGACGACGAGCAGCTGAGAG+AGG - tig0019825:119748-119767 Msa1376630:CDS 60.0%
!! GGTAGTTGCTCTGAGGGAGG+GGG + tig0019825:119890-119909 None:intergenic 60.0%
!! GGGTAGTTGCTCTGAGGGAG+GGG + tig0019825:119891-119910 None:intergenic 60.0%
!! GGGGTAGTTGCTCTGAGGGA+GGG + tig0019825:119892-119911 None:intergenic 60.0%
!! TGGGGTAGTTGCTCTGAGGG+AGG + tig0019825:119893-119912 None:intergenic 60.0%
GTATCGGGTGCAGCAGCAAG+TGG + tig0019825:119932-119951 None:intergenic 60.0%
ACCCCCCTCATTTGGGGGAA+TGG + tig0019825:119954-119973 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0019825 gene 119003 120320 119003 ID=Msa1376630;Name=Msa1376630
tig0019825 mRNA 119003 120320 119003 ID=Msa1376630-mRNA-1;Parent=Msa1376630;Name=Msa1376630-mRNA-1;_AED=0.02;_eAED=0.02;_QI=115|1|1|1|1|1|2|341|193
tig0019825 exon 119003 119816 119003 ID=Msa1376630-mRNA-1:exon:5216;Parent=Msa1376630-mRNA-1
tig0019825 exon 120097 120320 120097 ID=Msa1376630-mRNA-1:exon:5215;Parent=Msa1376630-mRNA-1
tig0019825 five_prime_UTR 120206 120320 120206 ID=Msa1376630-mRNA-1:five_prime_utr;Parent=Msa1376630-mRNA-1
tig0019825 CDS 120097 120205 120097 ID=Msa1376630-mRNA-1:cds;Parent=Msa1376630-mRNA-1
tig0019825 CDS 119344 119816 119344 ID=Msa1376630-mRNA-1:cds;Parent=Msa1376630-mRNA-1
tig0019825 three_prime_UTR 119003 119343 119003 ID=Msa1376630-mRNA-1:three_prime_utr;Parent=Msa1376630-mRNA-1
Gene Sequence

>Msa1376630

ATGAGCGCGGTTAACATTACGAACGTGACGGTCCTTGACAATCCAGCTTCGTTTCTCAATCCCTTTCAGTTCGAGATTTCCTATGAGTGTTTGGCCGCTCTCAAAGATGATTTGGAATGGAAGCTGATTTATGTTGGATCTGCTGAGGATGAGACTTATGACCAGTTATTGGAGAGTGTCCTTGTTGGTCCTGTCAATGTTGGAAACTATCGCTTTGTTTTACAGGCAGATCCACCAGACCCATCCAAGATTCGTGAAGAAGATATCATTGGTGTTACAGTGCTTCTACTCACATGCTCTTATTTGGGTCAAGAGTTTATTCGTGTTGGCTACTATGTCAACAATGACTATGACGACGAGCAGCTGAGAGAGGAACCTCCAACCAAGGTTTTAACAGATAGGGTTCAAAGGAACATCTTATCTGATAAACCAAGGGTCACTAAGTTCCCCATCAATTTCCACCCTGAGAACAATGAAAATGAAGAACAACCCCCTCCCTCAGAGCAACTACCCCAAACTGGAGAACAAGATCCACTTGCTGCTGCACCCGATACCATTCCCCCAAATGAGGGGGGTTCTTAA
Protein sequence

>Msa1376630

MSAVNITNVTVLDNPASFLNPFQFEISYECLAALKDDLEWKLIYVGSAEDETYDQLLESVLVGPVNVGNYRFVLQADPPDPSKIREEDIIGVTVLLLTCSYLGQEFIRVGYYVNNDYDDEQLREEPPTKVLTDRVQRNILSDKPRVTKFPINFHPENNENEEQPPPSEQLPQTGEQDPLAAAPDTIPPNEGGS*