Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1376870 | XP_024634987.1 | 97.561 | 82 | 2 | 0 | 1 | 82 | 1 | 82 | 1.52e-51 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1376870 | A0A072V0P5 | 97.561 | 82 | 2 | 0 | 1 | 82 | 1 | 82 | 2.46e-51 | 165 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0418220 | Msa1376870 | 0.807384 | 5.189485e-50 | -8.615850e-47 |
| Msa0464630 | Msa1376870 | 0.853798 | 1.883078e-61 | -8.615850e-47 |
| Msa0510440 | Msa1376870 | 0.914730 | 1.360349e-84 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1376870 | MtrunA17_Chr3g0115811 | 97.561 | 82 | 2 | 0 | 1 | 82 | 1 | 82 | 4.73e-55 | 165 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1376870 | AT1G34640.1 | 58.537 | 82 | 32 | 1 | 1 | 80 | 1 | 82 | 6.14e-23 | 84.7 |
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGTATTGTTGGAATTTC+TGG | 0.095978 | tig0019834:-1140 | Msa1376870:CDS |
| GATCTTTCCTCAACAGTAAC+AGG | 0.350655 | tig0019834:+1062 | None:intergenic |
| TTGGCTGGCAGCAGTTATAT+TGG | 0.382907 | tig0019834:-1213 | Msa1376870:CDS |
| ACCTATGTGTTGGGTATTGT+TGG | 0.449040 | tig0019834:-1149 | Msa1376870:CDS |
| TCCTCAACAGTAACAGGATA+AGG | 0.457912 | tig0019834:+1068 | None:intergenic |
| ATGTTCGTTACCTATGTGTT+GGG | 0.458889 | tig0019834:-1158 | Msa1376870:CDS |
| CGGTTGAAATAATCCCAATC+AGG | 0.486481 | tig0019834:+1107 | None:intergenic |
| TCAGCAGCTTCTCTTTCTCA+TGG | 0.490405 | tig0019834:-1041 | Msa1376870:CDS |
| CTCTAAGAACCTCTATACTT+TGG | 0.491621 | tig0019834:-1232 | Msa1376870:CDS |
| GCCTTATCCTGTTACTGTTG+AGG | 0.496290 | tig0019834:-1069 | Msa1376870:CDS |
| GCAGCAGTTATATTGGTAGT+TGG | 0.498983 | tig0019834:-1206 | Msa1376870:CDS |
| AAGAACCTCTATACTTTGGC+TGG | 0.505897 | tig0019834:-1228 | Msa1376870:CDS |
| GATGTTCGTTACCTATGTGT+TGG | 0.512795 | tig0019834:-1159 | Msa1376870:CDS |
| TAGAGCTGCATCATTTGCCA+TGG | 0.517008 | tig0019834:+1250 | None:intergenic |
| TGCTGCCAGCCAAAGTATAG+AGG | 0.518224 | tig0019834:+1223 | None:intergenic |
| TCAACCGTGATATTTCTCGT+TGG | 0.602906 | tig0019834:-1091 | Msa1376870:CDS |
| TCCAACAATACCCAACACAT+AGG | 0.663389 | tig0019834:+1148 | None:intergenic |
| AAGGCCAACGAGAAATATCA+CGG | 0.673257 | tig0019834:+1087 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTCTAAGAACCTCTATACTT+TGG | - | tig0019834:1038-1057 | Msa1376870:CDS | 35.0% |
| ATGTTCGTTACCTATGTGTT+GGG | - | tig0019834:1112-1131 | Msa1376870:CDS | 35.0% | |
| ! | TTGGGTATTGTTGGAATTTC+TGG | - | tig0019834:1130-1149 | Msa1376870:CDS | 35.0% |
| AAGAACCTCTATACTTTGGC+TGG | - | tig0019834:1042-1061 | Msa1376870:CDS | 40.0% | |
| ! | GCAGCAGTTATATTGGTAGT+TGG | - | tig0019834:1064-1083 | Msa1376870:CDS | 40.0% |
| GATGTTCGTTACCTATGTGT+TGG | - | tig0019834:1111-1130 | Msa1376870:CDS | 40.0% | |
| ! | ACCTATGTGTTGGGTATTGT+TGG | - | tig0019834:1121-1140 | Msa1376870:CDS | 40.0% |
| TCCAACAATACCCAACACAT+AGG | + | tig0019834:1125-1144 | None:intergenic | 40.0% | |
| ! | TGGTGTTCTTTTGCCTGATT+GGG | - | tig0019834:1150-1169 | Msa1376870:CDS | 40.0% |
| CGGTTGAAATAATCCCAATC+AGG | + | tig0019834:1166-1185 | None:intergenic | 40.0% | |
| TCAACCGTGATATTTCTCGT+TGG | - | tig0019834:1179-1198 | Msa1376870:CDS | 40.0% | |
| AAGGCCAACGAGAAATATCA+CGG | + | tig0019834:1186-1205 | None:intergenic | 40.0% | |
| TCCTCAACAGTAACAGGATA+AGG | + | tig0019834:1205-1224 | None:intergenic | 40.0% | |
| GATCTTTCCTCAACAGTAAC+AGG | + | tig0019834:1211-1230 | None:intergenic | 40.0% | |
| TTGGCTGGCAGCAGTTATAT+TGG | - | tig0019834:1057-1076 | Msa1376870:CDS | 45.0% | |
| ! | CTGGTGTTCTTTTGCCTGAT+TGG | - | tig0019834:1149-1168 | Msa1376870:CDS | 45.0% |
| GCCTTATCCTGTTACTGTTG+AGG | - | tig0019834:1201-1220 | Msa1376870:CDS | 45.0% | |
| TCAGCAGCTTCTCTTTCTCA+TGG | - | tig0019834:1229-1248 | Msa1376870:CDS | 45.0% | |
| TGCTGCCAGCCAAAGTATAG+AGG | + | tig0019834:1050-1069 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0019834 | gene | 1022 | 1270 | 1022 | ID=Msa1376870;Name=Msa1376870 |
| tig0019834 | mRNA | 1022 | 1270 | 1022 | ID=Msa1376870-mRNA-1;Parent=Msa1376870;Name=Msa1376870-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|82 |
| tig0019834 | exon | 1022 | 1270 | 1022 | ID=Msa1376870-mRNA-1:exon:5374;Parent=Msa1376870-mRNA-1 |
| tig0019834 | CDS | 1022 | 1270 | 1022 | ID=Msa1376870-mRNA-1:cds;Parent=Msa1376870-mRNA-1 |
| Gene Sequence |
| Protein sequence |
