Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1376970 | KEH40053.1 | 88.462 | 104 | 12 | 0 | 1 | 104 | 1 | 104 | 8.55e-63 | 196 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1376970 | A0A072VF29 | 88.462 | 104 | 12 | 0 | 1 | 104 | 1 | 104 | 4.08e-63 | 196 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0051560 | Msa1376970 | 0.922200 | 1.339314e-88 | -8.615850e-47 |
| Msa0051680 | Msa1376970 | 0.926445 | 4.641728e-91 | -8.615850e-47 |
| Msa0101910 | Msa1376970 | 0.975991 | 5.585680e-141 | -8.615850e-47 |
| Msa0146140 | Msa1376970 | 0.966669 | 3.134089e-126 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1376970 | MtrunA17_Chr1g0153841 | 88.462 | 104 | 12 | 0 | 1 | 104 | 1 | 104 | 7.87e-67 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTCGCCAACATGTGGATTC+TGG | 0.218060 | tig0019847:-17765 | None:intergenic |
| TGGTGTCCATGGGGATTGTT+GGG | 0.291649 | tig0019847:-17858 | None:intergenic |
| TTGGTGTCCATGGGGATTGT+TGG | 0.338734 | tig0019847:-17859 | None:intergenic |
| TGGAGGCACGTTGGTGTCCA+TGG | 0.365721 | tig0019847:-17869 | None:intergenic |
| TGGGGATTGTTGGGGTTGAA+TGG | 0.408417 | tig0019847:-17849 | None:intergenic |
| GTCGCCAACATGTGGATTCT+GGG | 0.412404 | tig0019847:-17764 | None:intergenic |
| CACCTAATTAATTAACGCCT+TGG | 0.435077 | tig0019847:-17889 | None:intergenic |
| CATATGAAAACATAGTCATT+TGG | 0.452470 | tig0019847:+17698 | Msa1376970:CDS |
| TATGTTTCTCAACCCTTGCT+AGG | 0.462334 | tig0019847:+17635 | Msa1376970:CDS |
| TGGGGTTGAATGGCCAGCAC+CGG | 0.464105 | tig0019847:-17839 | None:intergenic |
| GGAGGCACGTTGGTGTCCAT+GGG | 0.465448 | tig0019847:-17868 | None:intergenic |
| CTAATTAATTAACGCCTTGG+AGG | 0.484883 | tig0019847:-17886 | None:intergenic |
| CCAGGTCATAGTCCCGGTGC+TGG | 0.508743 | tig0019847:+17826 | Msa1376970:CDS |
| GGGGTTGAATGGCCAGCACC+GGG | 0.545577 | tig0019847:-17838 | None:intergenic |
| TGGACACCAACGTGCCTCCA+AGG | 0.558768 | tig0019847:+17872 | Msa1376970:CDS |
| GGACTATGACCTGGATCCGT+TGG | 0.561199 | tig0019847:-17817 | None:intergenic |
| TCAGCTTCGTCGCCAACATG+TGG | 0.570493 | tig0019847:-17772 | None:intergenic |
| ATGAAAACATAGTCATTTGG+CGG | 0.581045 | tig0019847:+17701 | Msa1376970:CDS |
| CCAGCACCGGGACTATGACC+TGG | 0.585779 | tig0019847:-17826 | None:intergenic |
| GGTGTCCATGGGGATTGTTG+GGG | 0.593694 | tig0019847:-17857 | None:intergenic |
| ACGGATCCAGGTCATAGTCC+CGG | 0.595399 | tig0019847:+17820 | Msa1376970:CDS |
| GATTCCCAGAATCCACATGT+TGG | 0.624961 | tig0019847:+17760 | Msa1376970:CDS |
| TTCAACCCCAACAATCCCCA+TGG | 0.630589 | tig0019847:+17852 | Msa1376970:CDS |
| GAGGCACGTTGGTGTCCATG+GGG | 0.682384 | tig0019847:-17867 | None:intergenic |
| TTAACGCCTTGGAGGCACGT+TGG | 0.691422 | tig0019847:-17878 | None:intergenic |
| GAAAAGTAGAATTGAACACA+AGG | 0.697977 | tig0019847:-17591 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGTTCTCTCTTTTTACTA+GGG | - | tig0019847:17662-17681 | None:intergenic | 25.0% |
| !! | ATTTGTTCTCTCTTTTTACT+AGG | - | tig0019847:17663-17682 | None:intergenic | 25.0% |
| ! | CATATGAAAACATAGTCATT+TGG | + | tig0019847:17698-17717 | Msa1376970:CDS | 25.0% |
| GAAAAGTAGAATTGAACACA+AGG | - | tig0019847:17594-17613 | None:intergenic | 30.0% | |
| ! | TTGTTCTCTCTTTTTACTAG+GGG | - | tig0019847:17661-17680 | None:intergenic | 30.0% |
| ATGAAAACATAGTCATTTGG+CGG | + | tig0019847:17701-17720 | Msa1376970:CDS | 30.0% | |
| TATGTTTCTCAACCCTTGCT+AGG | + | tig0019847:17635-17654 | Msa1376970:CDS | 40.0% | |
| ! | TTTTACTAGGGGCCTAGCAA+GGG | - | tig0019847:17650-17669 | None:intergenic | 45.0% |
| ! | TTTTTACTAGGGGCCTAGCA+AGG | - | tig0019847:17651-17670 | None:intergenic | 45.0% |
| GATTCCCAGAATCCACATGT+TGG | + | tig0019847:17760-17779 | Msa1376970:CDS | 45.0% | |
| ! | GATCGACGATTTTCGACCAA+CGG | + | tig0019847:17801-17820 | Msa1376970:CDS | 45.0% |
| GTCGCCAACATGTGGATTCT+GGG | - | tig0019847:17767-17786 | None:intergenic | 50.0% | |
| ! | GATTTTCGACCAACGGATCC+AGG | + | tig0019847:17808-17827 | Msa1376970:CDS | 50.0% |
| TGGGGATTGTTGGGGTTGAA+TGG | - | tig0019847:17852-17871 | None:intergenic | 50.0% | |
| TTCAACCCCAACAATCCCCA+TGG | + | tig0019847:17852-17871 | Msa1376970:CDS | 50.0% | |
| TGGTGTCCATGGGGATTGTT+GGG | - | tig0019847:17861-17880 | None:intergenic | 50.0% | |
| ! | TTGGTGTCCATGGGGATTGT+TGG | - | tig0019847:17862-17881 | None:intergenic | 50.0% |
| CGTCGCCAACATGTGGATTC+TGG | - | tig0019847:17768-17787 | None:intergenic | 55.0% | |
| TCAGCTTCGTCGCCAACATG+TGG | - | tig0019847:17775-17794 | None:intergenic | 55.0% | |
| GGACTATGACCTGGATCCGT+TGG | - | tig0019847:17820-17839 | None:intergenic | 55.0% | |
| ACGGATCCAGGTCATAGTCC+CGG | + | tig0019847:17820-17839 | Msa1376970:CDS | 55.0% | |
| GGTGTCCATGGGGATTGTTG+GGG | - | tig0019847:17860-17879 | None:intergenic | 55.0% | |
| ! | TTAACGCCTTGGAGGCACGT+TGG | - | tig0019847:17881-17900 | None:intergenic | 55.0% |
| TGGGGTTGAATGGCCAGCAC+CGG | - | tig0019847:17842-17861 | None:intergenic | 60.0% | |
| ! | GAGGCACGTTGGTGTCCATG+GGG | - | tig0019847:17870-17889 | None:intergenic | 60.0% |
| ! | GGAGGCACGTTGGTGTCCAT+GGG | - | tig0019847:17871-17890 | None:intergenic | 60.0% |
| ! | TGGAGGCACGTTGGTGTCCA+TGG | - | tig0019847:17872-17891 | None:intergenic | 60.0% |
| TGGACACCAACGTGCCTCCA+AGG | + | tig0019847:17872-17891 | Msa1376970:CDS | 60.0% | |
| ! | CCAGCACCGGGACTATGACC+TGG | - | tig0019847:17829-17848 | None:intergenic | 65.0% |
| CCAGGTCATAGTCCCGGTGC+TGG | + | tig0019847:17826-17845 | Msa1376970:CDS | 65.0% | |
| GGGGTTGAATGGCCAGCACC+GGG | - | tig0019847:17841-17860 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0019847 | gene | 17577 | 17900 | 17577 | ID=Msa1376970;Name=Msa1376970 |
| tig0019847 | mRNA | 17577 | 17900 | 17577 | ID=Msa1376970-mRNA-1;Parent=Msa1376970;Name=Msa1376970-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|107 |
| tig0019847 | exon | 17577 | 17900 | 17577 | ID=Msa1376970-mRNA-1:exon:5402;Parent=Msa1376970-mRNA-1 |
| tig0019847 | CDS | 17577 | 17900 | 17577 | ID=Msa1376970-mRNA-1:cds;Parent=Msa1376970-mRNA-1 |
| Gene Sequence |
| Protein sequence |
