Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0051560 | KEH40053.1 | 88.462 | 104 | 12 | 0 | 1 | 104 | 1 | 104 | 8.55e-63 | 196 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0051560 | A0A072VF29 | 88.462 | 104 | 12 | 0 | 1 | 104 | 1 | 104 | 4.08e-63 | 196 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0051560 | Msa0051680 | 0.937297 | 4.357617e-98 | -8.615850e-47 |
| Msa0051560 | Msa0101910 | 0.933348 | 2.151302e-95 | -8.615850e-47 |
| Msa0051560 | Msa0146140 | 0.924261 | 8.919272e-90 | -8.615850e-47 |
| Msa0051560 | Msa0817460 | 0.814916 | 1.207259e-51 | -8.615850e-47 |
| Msa0051560 | Msa0936060 | 0.803965 | 2.709228e-49 | -8.615850e-47 |
| Msa0051560 | Msa0972960 | 0.803030 | 4.234222e-49 | -8.615850e-47 |
| Msa0051560 | Msa1376970 | 0.922200 | 1.339314e-88 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0051560 | MtrunA17_Chr1g0153841 | 88.462 | 104 | 12 | 0 | 1 | 104 | 1 | 104 | 7.87e-67 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTCGCCAACATGTGGATTC+TGG | 0.218060 | 1_2:+5664466 | None:intergenic |
| TGGTGTCCATGGGGATTGTT+GGG | 0.291649 | 1_2:+5664373 | None:intergenic |
| TTGGTGTCCATGGGGATTGT+TGG | 0.338734 | 1_2:+5664372 | None:intergenic |
| TGGAGGCACGTTGGTGTCCA+TGG | 0.365721 | 1_2:+5664362 | None:intergenic |
| TGGGGATTGTTGGGGTTGAA+TGG | 0.408417 | 1_2:+5664382 | None:intergenic |
| GTCGCCAACATGTGGATTCT+GGG | 0.412404 | 1_2:+5664467 | None:intergenic |
| CACCTAATTAATTAACGCCT+TGG | 0.435077 | 1_2:+5664342 | None:intergenic |
| CATATGAAAACATAGTCATT+TGG | 0.452470 | 1_2:-5664533 | Msa0051560:CDS |
| TATGTTTCTCAACCCTTGCT+AGG | 0.462334 | 1_2:-5664596 | Msa0051560:CDS |
| TGGGGTTGAATGGCCAGCAC+CGG | 0.464105 | 1_2:+5664392 | None:intergenic |
| GGAGGCACGTTGGTGTCCAT+GGG | 0.465448 | 1_2:+5664363 | None:intergenic |
| CTAATTAATTAACGCCTTGG+AGG | 0.484883 | 1_2:+5664345 | None:intergenic |
| CCAGGTCATAGTCCCGGTGC+TGG | 0.508743 | 1_2:-5664405 | Msa0051560:CDS |
| GGGGTTGAATGGCCAGCACC+GGG | 0.545577 | 1_2:+5664393 | None:intergenic |
| TGGACACCAACGTGCCTCCA+AGG | 0.558768 | 1_2:-5664359 | Msa0051560:CDS |
| GGACTATGACCTGGATCCGT+TGG | 0.561199 | 1_2:+5664414 | None:intergenic |
| TCAGCTTCGTCGCCAACATG+TGG | 0.570493 | 1_2:+5664459 | None:intergenic |
| ATGAAAACATAGTCATTTGG+CGG | 0.581045 | 1_2:-5664530 | Msa0051560:CDS |
| CCAGCACCGGGACTATGACC+TGG | 0.585779 | 1_2:+5664405 | None:intergenic |
| GGTGTCCATGGGGATTGTTG+GGG | 0.593694 | 1_2:+5664374 | None:intergenic |
| ACGGATCCAGGTCATAGTCC+CGG | 0.595399 | 1_2:-5664411 | Msa0051560:CDS |
| GATTCCCAGAATCCACATGT+TGG | 0.624961 | 1_2:-5664471 | Msa0051560:CDS |
| TTCAACCCCAACAATCCCCA+TGG | 0.630589 | 1_2:-5664379 | Msa0051560:CDS |
| GAGGCACGTTGGTGTCCATG+GGG | 0.682384 | 1_2:+5664364 | None:intergenic |
| TTAACGCCTTGGAGGCACGT+TGG | 0.691422 | 1_2:+5664353 | None:intergenic |
| GAAAAGTAGAATTGAACACA+AGG | 0.697977 | 1_2:+5664640 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGTTCTCTCTTTTTACTA+GGG | + | chr1_2:5664438-5664457 | None:intergenic | 25.0% |
| !! | ATTTGTTCTCTCTTTTTACT+AGG | + | chr1_2:5664439-5664458 | None:intergenic | 25.0% |
| ! | CATATGAAAACATAGTCATT+TGG | - | chr1_2:5664474-5664493 | Msa0051560:CDS | 25.0% |
| GAAAAGTAGAATTGAACACA+AGG | + | chr1_2:5664370-5664389 | None:intergenic | 30.0% | |
| ! | TTGTTCTCTCTTTTTACTAG+GGG | + | chr1_2:5664437-5664456 | None:intergenic | 30.0% |
| ATGAAAACATAGTCATTTGG+CGG | - | chr1_2:5664477-5664496 | Msa0051560:CDS | 30.0% | |
| TATGTTTCTCAACCCTTGCT+AGG | - | chr1_2:5664411-5664430 | Msa0051560:CDS | 40.0% | |
| ! | TTTTACTAGGGGCCTAGCAA+GGG | + | chr1_2:5664426-5664445 | None:intergenic | 45.0% |
| ! | TTTTTACTAGGGGCCTAGCA+AGG | + | chr1_2:5664427-5664446 | None:intergenic | 45.0% |
| GATTCCCAGAATCCACATGT+TGG | - | chr1_2:5664536-5664555 | Msa0051560:CDS | 45.0% | |
| ! | GATCGACGATTTTCGACCAA+CGG | - | chr1_2:5664577-5664596 | Msa0051560:CDS | 45.0% |
| GTCGCCAACATGTGGATTCT+GGG | + | chr1_2:5664543-5664562 | None:intergenic | 50.0% | |
| ! | GATTTTCGACCAACGGATCC+AGG | - | chr1_2:5664584-5664603 | Msa0051560:CDS | 50.0% |
| TGGGGATTGTTGGGGTTGAA+TGG | + | chr1_2:5664628-5664647 | None:intergenic | 50.0% | |
| TTCAACCCCAACAATCCCCA+TGG | - | chr1_2:5664628-5664647 | Msa0051560:CDS | 50.0% | |
| TGGTGTCCATGGGGATTGTT+GGG | + | chr1_2:5664637-5664656 | None:intergenic | 50.0% | |
| ! | TTGGTGTCCATGGGGATTGT+TGG | + | chr1_2:5664638-5664657 | None:intergenic | 50.0% |
| CGTCGCCAACATGTGGATTC+TGG | + | chr1_2:5664544-5664563 | None:intergenic | 55.0% | |
| TCAGCTTCGTCGCCAACATG+TGG | + | chr1_2:5664551-5664570 | None:intergenic | 55.0% | |
| GGACTATGACCTGGATCCGT+TGG | + | chr1_2:5664596-5664615 | None:intergenic | 55.0% | |
| ACGGATCCAGGTCATAGTCC+CGG | - | chr1_2:5664596-5664615 | Msa0051560:CDS | 55.0% | |
| GGTGTCCATGGGGATTGTTG+GGG | + | chr1_2:5664636-5664655 | None:intergenic | 55.0% | |
| ! | TTAACGCCTTGGAGGCACGT+TGG | + | chr1_2:5664657-5664676 | None:intergenic | 55.0% |
| TGGGGTTGAATGGCCAGCAC+CGG | + | chr1_2:5664618-5664637 | None:intergenic | 60.0% | |
| ! | GAGGCACGTTGGTGTCCATG+GGG | + | chr1_2:5664646-5664665 | None:intergenic | 60.0% |
| ! | GGAGGCACGTTGGTGTCCAT+GGG | + | chr1_2:5664647-5664666 | None:intergenic | 60.0% |
| ! | TGGAGGCACGTTGGTGTCCA+TGG | + | chr1_2:5664648-5664667 | None:intergenic | 60.0% |
| TGGACACCAACGTGCCTCCA+AGG | - | chr1_2:5664648-5664667 | Msa0051560:CDS | 60.0% | |
| ! | CCAGCACCGGGACTATGACC+TGG | + | chr1_2:5664605-5664624 | None:intergenic | 65.0% |
| CCAGGTCATAGTCCCGGTGC+TGG | - | chr1_2:5664602-5664621 | Msa0051560:CDS | 65.0% | |
| GGGGTTGAATGGCCAGCACC+GGG | + | chr1_2:5664617-5664636 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 5664353 | 5664676 | 5664353 | ID=Msa0051560;Name=Msa0051560 |
| chr1_2 | mRNA | 5664353 | 5664676 | 5664353 | ID=Msa0051560-mRNA-1;Parent=Msa0051560;Name=Msa0051560-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|107 |
| chr1_2 | exon | 5664353 | 5664676 | 5664353 | ID=Msa0051560-mRNA-1:exon:1748;Parent=Msa0051560-mRNA-1 |
| chr1_2 | CDS | 5664353 | 5664676 | 5664353 | ID=Msa0051560-mRNA-1:cds;Parent=Msa0051560-mRNA-1 |
| Gene Sequence |
| Protein sequence |
