Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0051680 | KEH40053.1 | 88.462 | 104 | 12 | 0 | 1 | 104 | 1 | 104 | 8.55e-63 | 196 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0051680 | A0A072VF29 | 88.462 | 104 | 12 | 0 | 1 | 104 | 1 | 104 | 4.08e-63 | 196 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0036140 | Msa0051680 | 0.800657 | 1.300362e-48 | -8.615850e-47 |
| Msa0051560 | Msa0051680 | 0.937297 | 4.357617e-98 | -8.615850e-47 |
| Msa0051680 | Msa0101910 | 0.928220 | 3.930458e-92 | -8.615850e-47 |
| Msa0051680 | Msa0146140 | 0.937556 | 2.857243e-98 | -8.615850e-47 |
| Msa0051680 | Msa0661650 | 0.801753 | 7.760203e-49 | -8.615850e-47 |
| Msa0051680 | Msa0817460 | 0.818921 | 1.522571e-52 | -8.615850e-47 |
| Msa0051680 | Msa0936060 | 0.800331 | 1.515123e-48 | -8.615850e-47 |
| Msa0051680 | Msa1129730 | 0.807537 | 4.815311e-50 | -8.615850e-47 |
| Msa0051680 | Msa1331000 | 0.802823 | 4.671472e-49 | -8.615850e-47 |
| Msa0051680 | Msa1376970 | 0.926445 | 4.641728e-91 | -8.615850e-47 |
| Msa0051680 | Msa1435270 | 0.812473 | 4.165799e-51 | -8.615850e-47 |
| Msa0051680 | Msa1440560 | 0.804668 | 1.934679e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0051680 | MtrunA17_Chr1g0153841 | 88.462 | 104 | 12 | 0 | 1 | 104 | 1 | 104 | 7.87e-67 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTCGCCAACATGTGGATTC+TGG | 0.218060 | 1_2:+5786464 | None:intergenic |
| TGGTGTCCATGGGGATTGTT+GGG | 0.291649 | 1_2:+5786371 | None:intergenic |
| TTGGTGTCCATGGGGATTGT+TGG | 0.338734 | 1_2:+5786370 | None:intergenic |
| TGGAGGCACGTTGGTGTCCA+TGG | 0.365721 | 1_2:+5786360 | None:intergenic |
| TGGGGATTGTTGGGGTTGAA+TGG | 0.408417 | 1_2:+5786380 | None:intergenic |
| GTCGCCAACATGTGGATTCT+GGG | 0.412404 | 1_2:+5786465 | None:intergenic |
| CACCTAATTAATTAACGCCT+TGG | 0.435077 | 1_2:+5786340 | None:intergenic |
| CATATGAAAACATAGTCATT+TGG | 0.452470 | 1_2:-5786531 | Msa0051680:CDS |
| TATGTTTCTCAACCCTTGCT+AGG | 0.462334 | 1_2:-5786594 | Msa0051680:CDS |
| TGGGGTTGAATGGCCAGCAC+CGG | 0.464105 | 1_2:+5786390 | None:intergenic |
| GGAGGCACGTTGGTGTCCAT+GGG | 0.465448 | 1_2:+5786361 | None:intergenic |
| CTAATTAATTAACGCCTTGG+AGG | 0.484883 | 1_2:+5786343 | None:intergenic |
| CCAGGTCATAGTCCCGGTGC+TGG | 0.508743 | 1_2:-5786403 | Msa0051680:CDS |
| GGGGTTGAATGGCCAGCACC+GGG | 0.545577 | 1_2:+5786391 | None:intergenic |
| TGGACACCAACGTGCCTCCA+AGG | 0.558768 | 1_2:-5786357 | Msa0051680:CDS |
| GGACTATGACCTGGATCCGT+TGG | 0.561199 | 1_2:+5786412 | None:intergenic |
| TCAGCTTCGTCGCCAACATG+TGG | 0.570493 | 1_2:+5786457 | None:intergenic |
| ATGAAAACATAGTCATTTGG+CGG | 0.581045 | 1_2:-5786528 | Msa0051680:CDS |
| CCAGCACCGGGACTATGACC+TGG | 0.585779 | 1_2:+5786403 | None:intergenic |
| GGTGTCCATGGGGATTGTTG+GGG | 0.593694 | 1_2:+5786372 | None:intergenic |
| ACGGATCCAGGTCATAGTCC+CGG | 0.595399 | 1_2:-5786409 | Msa0051680:CDS |
| GATTCCCAGAATCCACATGT+TGG | 0.624961 | 1_2:-5786469 | Msa0051680:CDS |
| TTCAACCCCAACAATCCCCA+TGG | 0.630589 | 1_2:-5786377 | Msa0051680:CDS |
| GAGGCACGTTGGTGTCCATG+GGG | 0.682384 | 1_2:+5786362 | None:intergenic |
| TTAACGCCTTGGAGGCACGT+TGG | 0.691422 | 1_2:+5786351 | None:intergenic |
| GAAAAGTAGAATTGAACACA+AGG | 0.697977 | 1_2:+5786638 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGTTCTCTCTTTTTACTA+GGG | + | chr1_2:5786436-5786455 | None:intergenic | 25.0% |
| !! | ATTTGTTCTCTCTTTTTACT+AGG | + | chr1_2:5786437-5786456 | None:intergenic | 25.0% |
| ! | CATATGAAAACATAGTCATT+TGG | - | chr1_2:5786472-5786491 | Msa0051680:CDS | 25.0% |
| GAAAAGTAGAATTGAACACA+AGG | + | chr1_2:5786368-5786387 | None:intergenic | 30.0% | |
| ! | TTGTTCTCTCTTTTTACTAG+GGG | + | chr1_2:5786435-5786454 | None:intergenic | 30.0% |
| ATGAAAACATAGTCATTTGG+CGG | - | chr1_2:5786475-5786494 | Msa0051680:CDS | 30.0% | |
| TATGTTTCTCAACCCTTGCT+AGG | - | chr1_2:5786409-5786428 | Msa0051680:CDS | 40.0% | |
| ! | TTTTACTAGGGGCCTAGCAA+GGG | + | chr1_2:5786424-5786443 | None:intergenic | 45.0% |
| ! | TTTTTACTAGGGGCCTAGCA+AGG | + | chr1_2:5786425-5786444 | None:intergenic | 45.0% |
| GATTCCCAGAATCCACATGT+TGG | - | chr1_2:5786534-5786553 | Msa0051680:CDS | 45.0% | |
| ! | GATCGACGATTTTCGACCAA+CGG | - | chr1_2:5786575-5786594 | Msa0051680:CDS | 45.0% |
| GTCGCCAACATGTGGATTCT+GGG | + | chr1_2:5786541-5786560 | None:intergenic | 50.0% | |
| ! | GATTTTCGACCAACGGATCC+AGG | - | chr1_2:5786582-5786601 | Msa0051680:CDS | 50.0% |
| TGGGGATTGTTGGGGTTGAA+TGG | + | chr1_2:5786626-5786645 | None:intergenic | 50.0% | |
| TTCAACCCCAACAATCCCCA+TGG | - | chr1_2:5786626-5786645 | Msa0051680:CDS | 50.0% | |
| TGGTGTCCATGGGGATTGTT+GGG | + | chr1_2:5786635-5786654 | None:intergenic | 50.0% | |
| ! | TTGGTGTCCATGGGGATTGT+TGG | + | chr1_2:5786636-5786655 | None:intergenic | 50.0% |
| CGTCGCCAACATGTGGATTC+TGG | + | chr1_2:5786542-5786561 | None:intergenic | 55.0% | |
| TCAGCTTCGTCGCCAACATG+TGG | + | chr1_2:5786549-5786568 | None:intergenic | 55.0% | |
| GGACTATGACCTGGATCCGT+TGG | + | chr1_2:5786594-5786613 | None:intergenic | 55.0% | |
| ACGGATCCAGGTCATAGTCC+CGG | - | chr1_2:5786594-5786613 | Msa0051680:CDS | 55.0% | |
| GGTGTCCATGGGGATTGTTG+GGG | + | chr1_2:5786634-5786653 | None:intergenic | 55.0% | |
| ! | TTAACGCCTTGGAGGCACGT+TGG | + | chr1_2:5786655-5786674 | None:intergenic | 55.0% |
| TGGGGTTGAATGGCCAGCAC+CGG | + | chr1_2:5786616-5786635 | None:intergenic | 60.0% | |
| ! | GAGGCACGTTGGTGTCCATG+GGG | + | chr1_2:5786644-5786663 | None:intergenic | 60.0% |
| ! | GGAGGCACGTTGGTGTCCAT+GGG | + | chr1_2:5786645-5786664 | None:intergenic | 60.0% |
| ! | TGGAGGCACGTTGGTGTCCA+TGG | + | chr1_2:5786646-5786665 | None:intergenic | 60.0% |
| TGGACACCAACGTGCCTCCA+AGG | - | chr1_2:5786646-5786665 | Msa0051680:CDS | 60.0% | |
| ! | CCAGCACCGGGACTATGACC+TGG | + | chr1_2:5786603-5786622 | None:intergenic | 65.0% |
| CCAGGTCATAGTCCCGGTGC+TGG | - | chr1_2:5786600-5786619 | Msa0051680:CDS | 65.0% | |
| GGGGTTGAATGGCCAGCACC+GGG | + | chr1_2:5786615-5786634 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 5786351 | 5786674 | 5786351 | ID=Msa0051680;Name=Msa0051680 |
| chr1_2 | mRNA | 5786351 | 5786674 | 5786351 | ID=Msa0051680-mRNA-1;Parent=Msa0051680;Name=Msa0051680-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|107 |
| chr1_2 | exon | 5786351 | 5786674 | 5786351 | ID=Msa0051680-mRNA-1:exon:1785;Parent=Msa0051680-mRNA-1 |
| chr1_2 | CDS | 5786351 | 5786674 | 5786351 | ID=Msa0051680-mRNA-1:cds;Parent=Msa0051680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
