Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1388740 | AES82602.2 | 86.076 | 79 | 11 | 0 | 1 | 79 | 1 | 79 | 2.85e-39 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1388740 | sp|Q8GT20|BEBT_TOBAC | 62.338 | 77 | 29 | 0 | 3 | 79 | 6 | 82 | 5.41e-26 | 101 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1388740 | G7L0T1 | 86.076 | 79 | 11 | 0 | 1 | 79 | 1 | 79 | 1.36e-39 | 137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1129130 | Msa1388740 | 0.814810 | 1.274769e-51 | -8.615850e-47 |
| Msa1129470 | Msa1388740 | 0.805077 | 1.588371e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1388740 | MtrunA17_Chr7g0273741 | 86.076 | 79 | 11 | 0 | 1 | 79 | 1 | 79 | 2.97e-43 | 137 |
| Msa1388740 | MtrunA17_Chr7g0273761 | 87.179 | 78 | 10 | 0 | 2 | 79 | 374 | 451 | 3.29e-42 | 145 |
| Msa1388740 | MtrunA17_Chr7g0273781 | 83.544 | 79 | 13 | 0 | 1 | 79 | 34 | 112 | 3.50e-41 | 140 |
| Msa1388740 | MtrunA17_Chr5g0433641 | 81.013 | 79 | 15 | 0 | 1 | 79 | 1 | 79 | 1.71e-39 | 135 |
| Msa1388740 | MtrunA17_Chr7g0273791 | 79.747 | 79 | 16 | 0 | 1 | 79 | 1 | 79 | 3.56e-38 | 131 |
| Msa1388740 | MtrunA17_Chr7g0273731 | 78.481 | 79 | 17 | 0 | 1 | 79 | 84 | 162 | 2.78e-37 | 130 |
| Msa1388740 | MtrunA17_Chr7g0273721 | 77.922 | 77 | 17 | 0 | 2 | 78 | 3 | 79 | 8.59e-36 | 125 |
| Msa1388740 | MtrunA17_Chr3g0098961 | 67.089 | 79 | 26 | 0 | 1 | 79 | 4 | 82 | 2.75e-33 | 112 |
| Msa1388740 | MtrunA17_Chr8g0353421 | 59.459 | 74 | 30 | 0 | 6 | 79 | 5 | 78 | 2.86e-28 | 98.6 |
| Msa1388740 | MtrunA17_Chr1g0165711 | 62.162 | 74 | 28 | 0 | 6 | 79 | 5 | 78 | 6.50e-27 | 100 |
| Msa1388740 | MtrunA17_Chr1g0165671 | 59.459 | 74 | 30 | 0 | 6 | 79 | 23 | 96 | 1.51e-26 | 100 |
| Msa1388740 | MtrunA17_Chr6g0471371 | 56.757 | 74 | 32 | 0 | 6 | 79 | 5 | 78 | 9.30e-25 | 90.9 |
| Msa1388740 | MtrunA17_Chr1g0165681 | 55.405 | 74 | 33 | 0 | 6 | 79 | 5 | 78 | 6.34e-24 | 92.8 |
| Msa1388740 | MtrunA17_Chr1g0165661 | 53.247 | 77 | 36 | 0 | 3 | 79 | 2 | 78 | 7.35e-24 | 92.8 |
| Msa1388740 | MtrunA17_Chr7g0273671 | 93.023 | 43 | 3 | 0 | 1 | 43 | 1 | 43 | 1.20e-23 | 84.3 |
| Msa1388740 | MtrunA17_Chr1g0191081 | 58.442 | 77 | 32 | 0 | 3 | 79 | 11 | 87 | 4.11e-23 | 90.5 |
| Msa1388740 | MtrunA17_Chr1g0165741 | 52.703 | 74 | 35 | 0 | 6 | 79 | 5 | 78 | 9.95e-23 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1388740 | AT5G17540.1 | 61.039 | 77 | 29 | 1 | 3 | 79 | 2 | 77 | 2.14e-24 | 94.7 |
| Msa1388740 | AT3G03480.1 | 58.974 | 78 | 31 | 1 | 2 | 79 | 11 | 87 | 5.54e-22 | 87.8 |
Find 18 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACATTTAAGAACCTTAAC+AGG | 0.260011 | tig0021157:-124327 | None:intergenic |
| GGGTCTTTCTCTACCCTTGA+TGG | 0.346824 | tig0021157:-124306 | None:intergenic |
| TAACGATAGATAAATATAAT+AGG | 0.351117 | tig0021157:-124279 | None:intergenic |
| AAAGCTGGTGGCACCAACTC+TGG | 0.474806 | tig0021157:-124189 | None:intergenic |
| TCTATCGTTACGAACCATCA+AGG | 0.503853 | tig0021157:+124287 | Msa1388740:CDS |
| AGTTTAACTTCGCGAGGAGT+GGG | 0.506066 | tig0021157:-124213 | None:intergenic |
| TCGCGAGGAGTGGGTAAAGC+TGG | 0.526362 | tig0021157:-124204 | None:intergenic |
| TAGTTTAACTTCGCGAGGAG+TGG | 0.531895 | tig0021157:-124214 | None:intergenic |
| GGTAGAGAAAGACCCTGTTA+AGG | 0.553972 | tig0021157:+124309 | Msa1388740:CDS |
| GCGGAGGTGCCAACCAGAGT+TGG | 0.564737 | tig0021157:+124171 | Msa1388740:CDS |
| CTGGTGGCACCAACTCTGGT+TGG | 0.572358 | tig0021157:-124185 | None:intergenic |
| GATAATAGTTTAACTTCGCG+AGG | 0.573152 | tig0021157:-124219 | None:intergenic |
| TCTGACATAGATGATCAAGA+AGG | 0.602444 | tig0021157:+124235 | Msa1388740:CDS |
| CTATCGTTACGAACCATCAA+GGG | 0.609999 | tig0021157:+124288 | Msa1388740:CDS |
| CGAGGAGTGGGTAAAGCTGG+TGG | 0.619470 | tig0021157:-124201 | None:intergenic |
| CACATTTAAGAACCTTAACA+GGG | 0.639455 | tig0021157:-124326 | None:intergenic |
| TGTTAATGTTCACAGCGCGG+AGG | 0.678502 | tig0021157:+124155 | Msa1388740:CDS |
| CATTGTTAATGTTCACAGCG+CGG | 0.734245 | tig0021157:+124152 | Msa1388740:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACGATAGATAAATATAAT+AGG | - | tig0021157:124277-124296 | None:intergenic | 15.0% |
| CACATTTAAGAACCTTAACA+GGG | - | tig0021157:124324-124343 | None:intergenic | 30.0% | |
| ! | GATAATAGTTTAACTTCGCG+AGG | - | tig0021157:124217-124236 | None:intergenic | 35.0% |
| TCTGACATAGATGATCAAGA+AGG | + | tig0021157:124235-124254 | Msa1388740:CDS | 35.0% | |
| GCACATTTAAGAACCTTAAC+AGG | - | tig0021157:124325-124344 | None:intergenic | 35.0% | |
| CATTGTTAATGTTCACAGCG+CGG | + | tig0021157:124152-124171 | Msa1388740:CDS | 40.0% | |
| TCTATCGTTACGAACCATCA+AGG | + | tig0021157:124287-124306 | Msa1388740:CDS | 40.0% | |
| CTATCGTTACGAACCATCAA+GGG | + | tig0021157:124288-124307 | Msa1388740:CDS | 40.0% | |
| ! | AGTTTAACTTCGCGAGGAGT+GGG | - | tig0021157:124211-124230 | None:intergenic | 45.0% |
| ! | TAGTTTAACTTCGCGAGGAG+TGG | - | tig0021157:124212-124231 | None:intergenic | 45.0% |
| GGTAGAGAAAGACCCTGTTA+AGG | + | tig0021157:124309-124328 | Msa1388740:CDS | 45.0% | |
| TGTTAATGTTCACAGCGCGG+AGG | + | tig0021157:124155-124174 | Msa1388740:CDS | 50.0% | |
| GGGTCTTTCTCTACCCTTGA+TGG | - | tig0021157:124304-124323 | None:intergenic | 50.0% | |
| AAAGCTGGTGGCACCAACTC+TGG | - | tig0021157:124187-124206 | None:intergenic | 55.0% | |
| CTGGTGGCACCAACTCTGGT+TGG | - | tig0021157:124183-124202 | None:intergenic | 60.0% | |
| CGAGGAGTGGGTAAAGCTGG+TGG | - | tig0021157:124199-124218 | None:intergenic | 60.0% | |
| TCGCGAGGAGTGGGTAAAGC+TGG | - | tig0021157:124202-124221 | None:intergenic | 60.0% | |
| !! | GCGGAGGTGCCAACCAGAGT+TGG | + | tig0021157:124171-124190 | Msa1388740:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021157 | gene | 124142 | 124378 | 124142 | ID=Msa1388740;Name=Msa1388740 |
| tig0021157 | mRNA | 124142 | 124378 | 124142 | ID=Msa1388740-mRNA-1;Parent=Msa1388740;Name=Msa1388740-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|79 |
| tig0021157 | exon | 124142 | 124378 | 124142 | ID=Msa1388740-mRNA-1:exon:11234;Parent=Msa1388740-mRNA-1 |
| tig0021157 | CDS | 124142 | 124378 | 124142 | ID=Msa1388740-mRNA-1:cds;Parent=Msa1388740-mRNA-1 |
| Gene Sequence |
| Protein sequence |
