AlfalfaGEDB | Msa1129470

M. sativa cultivar zhongmu-4 / Msa1129470

KEGG: K19861
GO: -
PFAM: PF02458.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1129470	RHN49367.1	90.291	309	30	0	147	455	32	340	0.0	589
Msa1129470	RHN49367.1	93.261	460	30	1	2	460	374	833	0.0	898

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1129470	sp\|Q6E593\|BEBT1_PETHY	55.773	459	198	3	3	460	6	460	0.0	532

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1129470	A0A396HEP6	90.291	309	30	0	147	455	32	340	0.0	589
Msa1129470	A0A396HEP6	93.261	460	30	1	2	460	374	833	0.0	898

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa1129470	0.841509	4.551680e-58	-8.615850e-47
Msa0002690	Msa1129470	0.805614	1.226269e-49	-8.615850e-47
Msa0002700	Msa1129470	0.819732	9.952185e-53	-8.615850e-47
Msa0003370	Msa1129470	0.846928	1.596015e-59	-8.615850e-47
Msa0003450	Msa1129470	0.825726	4.005374e-54	-8.615850e-47
Msa0003840	Msa1129470	-0.814116	1.814356e-51	-8.615850e-47
Msa0005500	Msa1129470	0.858848	6.215447e-63	-8.615850e-47
Msa0011060	Msa1129470	0.812616	3.876977e-51	-8.615850e-47
Msa0014970	Msa1129470	0.815082	1.109273e-51	-8.615850e-47
Msa0016830	Msa1129470	0.835900	1.282091e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1129470	MtrunA17_Chr7g0273761	93.261	460	30	1	2	460	374	833	0.0	898
Msa1129470	MtrunA17_Chr7g0273761	90.291	309	30	0	147	455	32	340	0.0	589
Msa1129470	MtrunA17_Chr7g0273781	77.826	460	101	1	1	460	34	492	0.0	765
Msa1129470	MtrunA17_Chr7g0273791	77.462	457	102	1	1	457	1	456	0.0	756
Msa1129470	MtrunA17_Chr5g0433641	76.304	460	109	0	1	460	1	460	0.0	741
Msa1129470	MtrunA17_Chr7g0273731	76.682	446	103	1	1	446	84	528	0.0	729
Msa1129470	MtrunA17_Chr7g0273721	73.261	460	122	1	2	460	3	462	0.0	715
Msa1129470	MtrunA17_Chr1g0165671	57.082	466	178	7	6	460	23	477	0.0	553
Msa1129470	MtrunA17_Chr1g0165661	53.132	463	200	5	6	460	5	458	1.19e-176	503
Msa1129470	MtrunA17_Chr1g0165681	52.573	447	206	6	6	449	5	448	2.31e-174	497

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1129470	AT5G17540.1	47.109	467	231	9	3	460	2	461	1.15e-144	422
Msa1129470	AT3G03480.1	44.667	450	231	9	2	443	11	450	3.96e-129	382
Msa1129470	AT3G48720.1	30.664	437	287	9	8	441	7	430	5.14e-71	231
Msa1129470	AT5G41040.2	32.715	431	271	10	14	438	21	438	2.51e-70	230
Msa1129470	AT5G41040.1	32.715	431	271	10	14	438	37	454	3.23e-70	230
Msa1129470	AT5G63560.1	30.942	446	280	10	1	439	1	425	6.21e-67	221
Msa1129470	AT1G27620.1	30.858	431	266	12	14	434	13	421	2.23e-56	193
Msa1129470	AT5G48930.1	29.639	415	258	11	41	438	33	430	1.74e-49	175
Msa1129470	AT1G03390.1	29.792	433	267	13	14	426	26	441	1.11e-48	173
Msa1129470	AT2G19070.1	30.023	433	259	14	36	438	29	447	1.80e-46	167

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Find 126 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCCTTTAGGTTATGCAG+TGG	0.626639	7_3:-81430891	Msa1129470:CDS
AAACCTCTTCATAACTTCAG+AGG	0.602162	7_3:+81430589	Msa1129460:CDS
AAACTAGGACCAATCGTTGT+AGG	0.458464	7_3:+81430527	Msa1129460:intron
AAAGCTGGTGGCACCAACTC+TGG	0.474806	7_3:+81432825	None:intergenic
AACCTTCCATTCAACCAGTA+TGG	0.403496	7_3:-81431306	Msa1129470:CDS
AACTTATTACCATAATAAAT+TGG	0.316387	7_3:+81430417	Msa1129460:intron
AAGAATGCAAAAGGAGGAGA+AGG	0.542764	7_3:-81430635	Msa1129470:CDS
AAGAATTAGGGAGGGAGCTA+GGG	0.488281	7_3:-81432625	Msa1129470:CDS
AAGCTTTGCGGAAACCCTTT+AGG	0.345123	7_3:-81430902	Msa1129470:CDS
AAGGTAGAGATAACAAAACT+AGG	0.584883	7_3:+81430512	Msa1129460:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CACATTTAAGAACCTTAACA+GGG	+	chr7_3:81430612-81430631	Msa1129460:CDS	30.0%
TGTTTATTACTATCCACTTG+CGG	-	chr7_3:81430648-81430667	Msa1129470:CDS	30.0%
GATGTAACACTTGATGAATT+TGG	-	chr7_3:81430751-81430770	Msa1129470:CDS	30.0%
CACAACTTTATGGACAAAAA+TGG	+	chr7_3:81430940-81430959	None:intergenic	30.0%
TCCAAAAAATAGTAGTTGGA+AGG	-	chr7_3:81431087-81431106	Msa1129470:CDS	30.0%
AGAAAGGCAAGTATATTGTT+TGG	+	chr7_3:81431667-81431686	None:intergenic	30.0%
TAACATGTAGAAAGTTCATG+TGG	+	chr7_3:81431769-81431788	None:intergenic	30.0%
ATCACATATACCAAACTGAT+TGG	-	chr7_3:81431793-81431812	Msa1129470:intron	30.0%
TCTTCCTTGTTGATATTTGA+CGG	+	chr7_3:81432089-81432108	None:intergenic	30.0%
GTGTAAAGAAGAAAGATTGA+TGG	+	chr7_3:81432133-81432152	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	81430314	81433005	81430314	ID=Msa1129470;Name=Msa1129470
chr7_3	mRNA	81430314	81433005	81430314	ID=Msa1129470-mRNA-1;Parent=Msa1129470;Name=Msa1129470-mRNA-1;_AED=0.02;_eAED=0.02;_QI=116\|1\|1\|1\|1\|1\|2\|194\|460
chr7_3	exon	81430314	81431449	81430314	ID=Msa1129470-mRNA-1:exon:23680;Parent=Msa1129470-mRNA-1
chr7_3	exon	81432449	81433005	81432449	ID=Msa1129470-mRNA-1:exon:23679;Parent=Msa1129470-mRNA-1
chr7_3	five_prime_UTR	81432890	81433005	81432890	ID=Msa1129470-mRNA-1:five_prime_utr;Parent=Msa1129470-mRNA-1
chr7_3	CDS	81432449	81432889	81432449	ID=Msa1129470-mRNA-1:cds;Parent=Msa1129470-mRNA-1
chr7_3	CDS	81430508	81431449	81430508	ID=Msa1129470-mRNA-1:cds;Parent=Msa1129470-mRNA-1
chr7_3	three_prime_UTR	81430314	81430507	81430314	ID=Msa1129470-mRNA-1:three_prime_utr;Parent=Msa1129470-mRNA-1

Gene Sequence

>Msa1129470

ATGACTTCGTCATTGTTAATGTTCACAGCGCGGAGGTGCCAACCAGAGTTGGTGCCACCAGCTTTACCCACTCCTCGCGAAGTTAAACTATTATCTGACATAGATGATCAAGAAGGTTTGCGTTTCAATATGCCTATTATATTTATCTATCGTTACGAACCATCAATGGTAGAGAAAGACCCTGTTAAGGTTCTTAAATGTGCACTCTCAAAAACACTTGTTTATTACTATCCACTTGCGGGAAGAATTAGGGAGGGAGCTAGGGACAAGTTGATGGTGGATTGTACTGGTGAAGGTGTCATGTTCATTGAAGCTGAAGCTGATGTAACACTTGATGAATTTGGTGATGTACCTCATCCCCCATTCCCTTGCTTCCAAGAACTCATATACGATGTTCCAGGCACGAAACAAATTATTGATCATCCTATTCTGTTCATACAGATCACACGTCTCAAGTGTGGTGGTTTCATTGTGGGCGTAAACTGGAACCATGCAATTGGCGATGCAACAGGTTTGAAACAATTCATGAATGTATGGGCAGAAATGGCTCGAGGTGCACATCAACCTTCCATTCAACCAGTATGGCGCAGAGAGATCCTAATGGCAAGAGACCCTCCGCGCATTACTTGCAACCATCGTGAATACCAACAAATACTTCCGTCAAATATCAACAAGGAAGAAGATAATGTTGCTACCATAGTCCATCAATCTTTCTTCTTTACACCTTCTCACATAGCTGCAATTCGTCACTTGATTCCATTTCACCTTCGTCAATGCAGTACATTTGATCTTATCACTGCATGTTTTTGGTGTTGTCGCACAAAAGCATTACAGCTAGAGCCAGATGAAGAAGTACAAATGATGTGCATCGTTAATGCACGTTCAAGGTTTAGGGCTAACCATTCGCCACTTGATGGATATTATGGTAACTGTTTTGCTTTCCCGGCTGCTGTCACCACCGCAGGGAAGCTTTGCGGAAACCCTTTAGGTTATGCAGTGGAAATGATAAGAAAAGCAAAAGCTGAAGTTACAGAAGAATACATGCATTCTTTGGCAGATCTTATGGTTATTAAGGAACGATGTTTATTTACAACAATAAGAGCATGTGTTGTATCAGACTTGACTCGTGCTAAGTACTCAGAAGTAAATTTTGGATGGGGTGAGGCGGTGTATGGTGGAGTGGTAAAAGGTGGGGCTGGACCTTTACCTGGAGCTACGTATATAATCCCACATAAGAATGCAAAAGGAGGAGAAGGTTTGATATTATTAATTTTCTTAACCTCTGAAGTTATGAAGAGGTTTGCTGAAGAGTTAGATAAGATGTTTGGTAACCAAAATCAACCTACAACGATTGGTCCTAGTTTTGTTATCTCTACCTTATAG

Protein sequence

>Msa1129470

MTSSLLMFTARRCQPELVPPALPTPREVKLLSDIDDQEGLRFNMPIIFIYRYEPSMVEKDPVKVLKCALSKTLVYYYPLAGRIREGARDKLMVDCTGEGVMFIEAEADVTLDEFGDVPHPPFPCFQELIYDVPGTKQIIDHPILFIQITRLKCGGFIVGVNWNHAIGDATGLKQFMNVWAEMARGAHQPSIQPVWRREILMARDPPRITCNHREYQQILPSNINKEEDNVATIVHQSFFFTPSHIAAIRHLIPFHLRQCSTFDLITACFWCCRTKALQLEPDEEVQMMCIVNARSRFRANHSPLDGYYGNCFAFPAAVTTAGKLCGNPLGYAVEMIRKAKAEVTEEYMHSLADLMVIKERCLFTTIRACVVSDLTRAKYSEVNFGWGEAVYGGVVKGGAGPLPGATYIIPHKNAKGGEGLILLIFLTSEVMKRFAEELDKMFGNQNQPTTIGPSFVISTL*