Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389240 | KEH40566.1 | 57.895 | 95 | 21 | 4 | 2 | 83 | 740 | 828 | 3.01e-15 | 80.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389240 | A0A072VG29 | 57.895 | 95 | 21 | 4 | 2 | 83 | 740 | 828 | 1.44e-15 | 80.5 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1389240 | Msa1423990 | 0.826625 | 2.448050e-54 | -8.615850e-47 |
| Msa1389240 | Msa1444370 | 0.815782 | 7.750234e-52 | -8.615850e-47 |
| Msa1249490 | Msa1389240 | 0.835241 | 1.881670e-56 | -8.615850e-47 |
| Msa0458700 | Msa1389240 | 0.815087 | 1.106123e-51 | -8.615850e-47 |
| Msa1002320 | Msa1389240 | 0.805807 | 1.116892e-49 | -8.615850e-47 |
| Msa0344830 | Msa1389240 | 0.801717 | 7.891902e-49 | -8.615850e-47 |
| Msa1136090 | Msa1389240 | 0.808101 | 3.654232e-50 | -8.615850e-47 |
| Msa0580610 | Msa1389240 | 0.820335 | 7.241048e-53 | -8.615850e-47 |
| Msa0590120 | Msa1389240 | 0.807702 | 4.441689e-50 | -8.615850e-47 |
| Msa0885270 | Msa1389240 | 0.817212 | 3.708073e-52 | -8.615850e-47 |
| Msa0885280 | Msa1389240 | 0.804869 | 1.756420e-49 | -8.615850e-47 |
| Msa0898380 | Msa1389240 | 0.805544 | 1.268552e-49 | -8.615850e-47 |
| Msa0898390 | Msa1389240 | 0.812909 | 3.344149e-51 | -8.615850e-47 |
| Msa0954910 | Msa1389240 | 0.809153 | 2.178112e-50 | -8.615850e-47 |
| Msa0964220 | Msa1389240 | 0.834294 | 3.257521e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGCATCGTGTTCATTCTT+TGG | 0.246372 | tig0021250:+37855 | None:intergenic |
| AAGCATCGTGTTCATTCTTT+GGG | 0.359597 | tig0021250:+37856 | None:intergenic |
| GCCATGCAAATTGATGATTA+TGG | 0.363303 | tig0021250:-37376 | Msa1389240:CDS |
| TTTGCATGGCACATGCAATC+TGG | 0.368924 | tig0021250:+37389 | None:intergenic |
| TGGGCATGGGAAGGTATATA+TGG | 0.394204 | tig0021250:+37409 | None:intergenic |
| CACCATTTGCAGAGCTCAAA+AGG | 0.398062 | tig0021250:-37442 | Msa1389240:CDS |
| TTGCATGGCACATGCAATCT+GGG | 0.489199 | tig0021250:+37390 | None:intergenic |
| TGCCACGGATCATGATATCA+TGG | 0.549241 | tig0021250:-37808 | Msa1389240:CDS |
| TGGCACATGCAATCTGGGCA+TGG | 0.551645 | tig0021250:+37395 | None:intergenic |
| TCCATAATCATCAATTTGCA+TGG | 0.563396 | tig0021250:+37375 | None:intergenic |
| GGCACATGCAATCTGGGCAT+GGG | 0.576360 | tig0021250:+37396 | None:intergenic |
| CTCCATGATATCATGATCCG+TGG | 0.589676 | tig0021250:+37806 | None:intergenic |
| TTTCAGTTCTGATTCTGCCA+CGG | 0.602513 | tig0021250:-37823 | Msa1389240:CDS |
| CATGCAATCTGGGCATGGGA+AGG | 0.612438 | tig0021250:+37400 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTAATTTAATACTTTATAGA+TGG | - | tig0021250:37743-37762 | Msa1389240:intron | 15.0% |
| !! | AATACTGAGAAAATAAACAA+CGG | - | tig0021250:37599-37618 | Msa1389240:intron | 20.0% |
| !! | GATCTTATCTTATTATTTGT+AGG | - | tig0021250:37708-37727 | Msa1389240:intron | 20.0% |
| !!! | AAAAGTGATTCTGAAGTTTT+TGG | - | tig0021250:37437-37456 | Msa1389240:CDS | 25.0% |
| ! | AATGTATTCCTCAATTTCAT+TGG | - | tig0021250:37523-37542 | Msa1389240:intron | 25.0% |
| !! | TTTTTCTTAGTATGCAAGTT+CGG | + | tig0021250:37630-37649 | None:intergenic | 25.0% |
| ! | TAAGAAAAATATAGCATCTG+AGG | - | tig0021250:37641-37660 | Msa1389240:intron | 25.0% |
| TCCATAATCATCAATTTGCA+TGG | + | tig0021250:37848-37867 | None:intergenic | 30.0% | |
| ! | AAGCATCGTGTTCATTCTTT+GGG | + | tig0021250:37367-37386 | None:intergenic | 35.0% |
| TCTATTCGCCAATGAAATTG+AGG | + | tig0021250:37534-37553 | None:intergenic | 35.0% | |
| AAAGAACACCTCCATCAATT+TGG | - | tig0021250:37560-37579 | Msa1389240:intron | 35.0% | |
| GAAAAATATAGCATCTGAGG+AGG | - | tig0021250:37644-37663 | Msa1389240:intron | 35.0% | |
| ATAAAGAACGCCCTTAACAA+GGG | + | tig0021250:37677-37696 | None:intergenic | 35.0% | |
| TATAAAGAACGCCCTTAACA+AGG | + | tig0021250:37678-37697 | None:intergenic | 35.0% | |
| ! | GCCATGCAAATTGATGATTA+TGG | - | tig0021250:37844-37863 | Msa1389240:CDS | 35.0% |
| !! | GAAGCATCGTGTTCATTCTT+TGG | + | tig0021250:37368-37387 | None:intergenic | 40.0% |
| ! | TTTCAGTTCTGATTCTGCCA+CGG | - | tig0021250:37397-37416 | Msa1389240:CDS | 40.0% |
| TTTCCCAGATGCCAAATTGA+TGG | + | tig0021250:37574-37593 | None:intergenic | 40.0% | |
| AGGAACTTTCCCCTTGTTAA+GGG | - | tig0021250:37664-37683 | Msa1389240:intron | 40.0% | |
| TAAAGAACGCCCTTAACAAG+GGG | + | tig0021250:37676-37695 | None:intergenic | 40.0% | |
| TGCCACGGATCATGATATCA+TGG | - | tig0021250:37412-37431 | Msa1389240:CDS | 45.0% | |
| CTCCATGATATCATGATCCG+TGG | + | tig0021250:37417-37436 | None:intergenic | 45.0% | |
| ACCTCCATCAATTTGGCATC+TGG | - | tig0021250:37567-37586 | Msa1389240:intron | 45.0% | |
| CCTCCATCAATTTGGCATCT+GGG | - | tig0021250:37568-37587 | Msa1389240:intron | 45.0% | |
| GAGGAACTTTCCCCTTGTTA+AGG | - | tig0021250:37663-37682 | Msa1389240:intron | 45.0% | |
| CACCATTTGCAGAGCTCAAA+AGG | - | tig0021250:37778-37797 | Msa1389240:CDS | 45.0% | |
| ! | TGCCTTTTGAGCTCTGCAAA+TGG | + | tig0021250:37783-37802 | None:intergenic | 45.0% |
| ! | TGGGCATGGGAAGGTATATA+TGG | + | tig0021250:37814-37833 | None:intergenic | 45.0% |
| TTGCATGGCACATGCAATCT+GGG | + | tig0021250:37833-37852 | None:intergenic | 45.0% | |
| TTTGCATGGCACATGCAATC+TGG | + | tig0021250:37834-37853 | None:intergenic | 45.0% | |
| CCCAGATGCCAAATTGATGG+AGG | + | tig0021250:37571-37590 | None:intergenic | 50.0% | |
| CATGCAATCTGGGCATGGGA+AGG | + | tig0021250:37823-37842 | None:intergenic | 55.0% | |
| GGCACATGCAATCTGGGCAT+GGG | + | tig0021250:37827-37846 | None:intergenic | 55.0% | |
| TGGCACATGCAATCTGGGCA+TGG | + | tig0021250:37828-37847 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021250 | gene | 37357 | 37885 | 37357 | ID=Msa1389240;Name=Msa1389240 |
| tig0021250 | mRNA | 37357 | 37885 | 37357 | ID=Msa1389240-mRNA-1;Parent=Msa1389240;Name=Msa1389240-mRNA-1;_AED=0.72;_eAED=1.00;_QI=0|0|0|0.5|0|0|2|0|83 |
| tig0021250 | exon | 37758 | 37885 | 37758 | ID=Msa1389240-mRNA-1:exon:11521;Parent=Msa1389240-mRNA-1 |
| tig0021250 | exon | 37357 | 37480 | 37357 | ID=Msa1389240-mRNA-1:exon:11520;Parent=Msa1389240-mRNA-1 |
| tig0021250 | CDS | 37758 | 37885 | 37758 | ID=Msa1389240-mRNA-1:cds;Parent=Msa1389240-mRNA-1 |
| tig0021250 | CDS | 37357 | 37480 | 37357 | ID=Msa1389240-mRNA-1:cds;Parent=Msa1389240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
