AlfalfaGEDB | Msa1390830

M. sativa cultivar zhongmu-4 / Msa1390830

KEGG: K01431
GO: GO:0003674 GO:0003824 GO:0003837 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006206 GO:0006208 GO:0006212 GO:0006725 GO:0006807 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009605 GO:0009987 GO:0009991 GO:0016787 GO:0016810 GO:0016811 GO:0017144 GO:0019860 GO:0031667 GO:0031668 GO:0031669 GO:0034641 GO:0042737 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046113 GO:0046483 GO:0046700 GO:0050896 GO:0051716 GO:0055086 GO:0071496 GO:0071704 GO:0072527 GO:0072529 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1390830	AES90587.2	86.842	76	10	0	5	80	26	101	9.71e-38	139

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1390830	sp\|Q8H183\|BUP1_ARATH	66.667	69	23	0	5	73	53	121	9.88e-24	95.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1390830	G7JV37	86.842	76	10	0	5	80	26	101	4.64e-38	139

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1265150	Msa1390830	0.869065	4.092104e-66	-8.615850e-47
Msa0312860	Msa1390830	0.949383	1.423413e-107	-8.615850e-47
Msa0319100	Msa1390830	0.843355	1.474918e-58	-8.615850e-47
Msa1126700	Msa1390830	0.825443	4.675697e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1390830	MtrunA17_Chr4g0049851	86.842	76	10	0	5	80	59	134	1.62e-41	139
Msa1390830	MtrunA17_Chr4g0049861	69.737	76	23	0	5	80	57	132	7.39e-33	116

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1390830	AT5G64370.1	66.667	69	23	0	5	73	53	121	1.00e-24	95.1

Find 33 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTACAGCCTCATGTGGATTT+TGG	0.130792	tig0021393:-11479	None:intergenic
ATGCTGATAAAGAAGTATTA+AGG	0.197682	tig0021393:-11064	Msa1390830:CDS
GAGAGAGCTGTGGCGGATTC+CGG	0.340791	tig0021393:+11350	None:intergenic
TCGATAAAGTGCGCAATTGT+TGG	0.373558	tig0021393:+10993	None:intergenic
TGCTGATAAAGAAGTATTAA+GGG	0.432567	tig0021393:-11063	Msa1390830:CDS
CGATAAAGTGCGCAATTGTT+GGG	0.445360	tig0021393:+10994	None:intergenic
TAATGAAACAATTGCTCTAC+CGG	0.451479	tig0021393:-11369	Msa1390830:CDS
TTAACAACAGAGTTCTGAAT+GGG	0.497423	tig0021393:+10939	None:intergenic
TTTGGGTTGGCGATATCTGG+AGG	0.509662	tig0021393:-11461	Msa1390830:five_prime_UTR
TGATGCTAGACCCTTCATAC+AGG	0.519583	tig0021393:+10898	None:intergenic
GCTAGACCCTTCATACAGGC+TGG	0.534194	tig0021393:+10902	None:intergenic
TTGGGTTGGCGATATCTGGA+GGG	0.536736	tig0021393:-11460	Msa1390830:five_prime_UTR
GTATGAAGGGTCTAGCATCA+TGG	0.543228	tig0021393:-10895	Msa1390830:three_prime_UTR
ATCCGCCACAGCTCTCTCCA+TGG	0.543560	tig0021393:-11345	Msa1390830:CDS
TATGAAGGGTCTAGCATCAT+GGG	0.551384	tig0021393:-10894	Msa1390830:three_prime_UTR
AAGGGAACCTCGAGTTGTGA+GGG	0.552694	tig0021393:-11045	Msa1390830:CDS
ATGTCTTCCAGCCTGTATGA+AGG	0.554066	tig0021393:-10909	Msa1390830:three_prime_UTR
GAACCTCGAGTTGTGAGGGT+TGG	0.560787	tig0021393:-11041	Msa1390830:CDS
GTTAACAACAGAGTTCTGAA+TGG	0.561492	tig0021393:+10938	None:intergenic
TGTCTTCCAGCCTGTATGAA+GGG	0.565467	tig0021393:-10908	Msa1390830:three_prime_UTR
GTTCAATTTACAGCCTCATG+TGG	0.577586	tig0021393:-11486	None:intergenic
GAGGTCAAAACCATGTTCCA+TGG	0.583571	tig0021393:+11328	None:intergenic
TTCCATGGAGAGAGCTGTGG+CGG	0.594000	tig0021393:+11343	None:intergenic
ACAGCTCTCTCCATGGAACA+TGG	0.598176	tig0021393:-11338	Msa1390830:CDS
TAAGGGAACCTCGAGTTGTG+AGG	0.598332	tig0021393:-11046	Msa1390830:CDS
AAAGTGCGCAATTGTTGGGA+GGG	0.605427	tig0021393:+10998	None:intergenic
AAACCAACCCTCACAACTCG+AGG	0.620062	tig0021393:+11038	None:intergenic
CTACAATATAATCATAATCG+CGG	0.620252	tig0021393:+11421	None:intergenic
TAAAGTGCGCAATTGTTGGG+AGG	0.630263	tig0021393:+10997	None:intergenic
TGGGTTGGCGATATCTGGAG+GGG	0.654158	tig0021393:-11459	Msa1390830:five_prime_UTR
ATGTTCCATGGAGAGAGCTG+TGG	0.669038	tig0021393:+11340	None:intergenic
GCCAACCCAAAATCCACATG+AGG	0.676621	tig0021393:+11473	None:intergenic
GAAGGGTCTAGCATCATGGG+AGG	0.706115	tig0021393:-10891	Msa1390830:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTAAGTTTATTTATTTC+AGG	-	tig0021393:11267-11286	Msa1390830:intron	10.0%
!!	TTTATCTTGTGATATCATTA+GGG	+	tig0021393:11089-11108	None:intergenic	20.0%
!!!	TTTTATCTTGTGATATCATT+AGG	+	tig0021393:11090-11109	None:intergenic	20.0%
!!	TAATGATATCACAAGATAAA+AGG	-	tig0021393:11089-11108	Msa1390830:intron	20.0%
!!!	GTTATTTAAGCTATAACTTT+TGG	-	tig0021393:11233-11252	Msa1390830:intron	20.0%
!	CTACAATATAATCATAATCG+CGG	+	tig0021393:10948-10967	None:intergenic	25.0%
!!!	ATGCTGATAAAGAAGTATTA+AGG	-	tig0021393:11302-11321	Msa1390830:intron	25.0%
!!!	TGCTGATAAAGAAGTATTAA+GGG	-	tig0021393:11303-11322	Msa1390830:intron	25.0%
	TAATGAAACAATTGCTCTAC+CGG	-	tig0021393:10997-11016	Msa1390830:CDS	30.0%
!!	TTAACAACAGAGTTCTGAAT+GGG	+	tig0021393:11430-11449	None:intergenic	30.0%
	TGAAGTGTATTCATACCTTG+AGG	+	tig0021393:11060-11079	None:intergenic	35.0%
!	TCACAAGATAAAAGGTATGC+TGG	-	tig0021393:11097-11116	Msa1390830:intron	35.0%
	ACAGATAAGTTTCAATGCTG+AGG	+	tig0021393:11215-11234	None:intergenic	35.0%
	GCACTTTATCGAGCAAAAAA+AGG	-	tig0021393:11384-11403	Msa1390830:exon	35.0%
!!	GTTAACAACAGAGTTCTGAA+TGG	+	tig0021393:11431-11450	None:intergenic	35.0%
	GCATACACTCCATCAGTTTA+AGG	+	tig0021393:11157-11176	None:intergenic	40.0%
	CGATAAAGTGCGCAATTGTT+GGG	+	tig0021393:11375-11394	None:intergenic	40.0%
	TCGATAAAGTGCGCAATTGT+TGG	+	tig0021393:11376-11395	None:intergenic	40.0%
	TATGAAGGGTCTAGCATCAT+GGG	-	tig0021393:11472-11491	Msa1390830:five_prime_UTR	40.0%
!!!	GATTTTGGGTTGGCGATATC+TGG	-	tig0021393:10902-10921	Msa1390830:three_prime_UTR	45.0%
	GAGGTCAAAACCATGTTCCA+TGG	+	tig0021393:11041-11060	None:intergenic	45.0%
!!!	GGAACATGGTTTTGACCTCA+AGG	-	tig0021393:11042-11061	Msa1390830:CDS	45.0%
!!	GCTGTGTTGCTTCCTTAAAC+TGG	-	tig0021393:11129-11148	Msa1390830:intron	45.0%
!	CAGTTTAAGGCGCCAGTTTA+AGG	+	tig0021393:11144-11163	None:intergenic	45.0%
	AAACTGGCGCCTTAAACTGA+TGG	-	tig0021393:11145-11164	Msa1390830:intron	45.0%
	TGCTCGATTTCTCGACAACA+CGG	-	tig0021393:11174-11193	Msa1390830:intron	45.0%
	AAAGTGCGCAATTGTTGGGA+GGG	+	tig0021393:11371-11390	None:intergenic	45.0%
	TAAAGTGCGCAATTGTTGGG+AGG	+	tig0021393:11372-11391	None:intergenic	45.0%
	ATGTCTTCCAGCCTGTATGA+AGG	-	tig0021393:11457-11476	Msa1390830:five_prime_UTR	45.0%
	TGTCTTCCAGCCTGTATGAA+GGG	-	tig0021393:11458-11477	Msa1390830:five_prime_UTR	45.0%
	TGATGCTAGACCCTTCATAC+AGG	+	tig0021393:11471-11490	None:intergenic	45.0%
	GTATGAAGGGTCTAGCATCA+TGG	-	tig0021393:11471-11490	Msa1390830:five_prime_UTR	45.0%
!!	GCCTCATGTGGATTTTGGGT+TGG	-	tig0021393:10892-10911	Msa1390830:three_prime_UTR	50.0%
	GCCAACCCAAAATCCACATG+AGG	+	tig0021393:10896-10915	None:intergenic	50.0%
!!	TTTGGGTTGGCGATATCTGG+AGG	-	tig0021393:10905-10924	Msa1390830:three_prime_UTR	50.0%
!!	TTGGGTTGGCGATATCTGGA+GGG	-	tig0021393:10906-10925	Msa1390830:three_prime_UTR	50.0%
	ATGTTCCATGGAGAGAGCTG+TGG	+	tig0021393:11029-11048	None:intergenic	50.0%
	ACAGCTCTCTCCATGGAACA+TGG	-	tig0021393:11028-11047	Msa1390830:CDS	50.0%
!	TAAGGGAACCTCGAGTTGTG+AGG	-	tig0021393:11320-11339	Msa1390830:intron	50.0%
!	AAGGGAACCTCGAGTTGTGA+GGG	-	tig0021393:11321-11340	Msa1390830:intron	50.0%
	AAACCAACCCTCACAACTCG+AGG	+	tig0021393:11331-11350	None:intergenic	50.0%
!!	TGGGTTGGCGATATCTGGAG+GGG	-	tig0021393:10907-10926	Msa1390830:three_prime_UTR	55.0%
	TTCCATGGAGAGAGCTGTGG+CGG	+	tig0021393:11026-11045	None:intergenic	55.0%
!	GAACCTCGAGTTGTGAGGGT+TGG	-	tig0021393:11325-11344	Msa1390830:CDS	55.0%
	GCTAGACCCTTCATACAGGC+TGG	+	tig0021393:11467-11486	None:intergenic	55.0%
!!	GAGAGAGCTGTGGCGGATTC+CGG	+	tig0021393:11019-11038	None:intergenic	60.0%
	ATCCGCCACAGCTCTCTCCA+TGG	-	tig0021393:11021-11040	Msa1390830:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0021393	gene	10891	11497	10891	ID=Msa1390830;Name=Msa1390830
tig0021393	mRNA	10891	11497	10891	ID=Msa1390830-mRNA-1;Parent=Msa1390830;Name=Msa1390830-mRNA-1;_AED=0.00;_eAED=0.00;_QI=98\|1\|1\|1\|1\|1\|2\|41\|80
tig0021393	exon	10891	11099	10891	ID=Msa1390830-mRNA-1:exon:12470;Parent=Msa1390830-mRNA-1
tig0021393	exon	11325	11497	11325	ID=Msa1390830-mRNA-1:exon:12469;Parent=Msa1390830-mRNA-1
tig0021393	five_prime_UTR	11400	11497	11400	ID=Msa1390830-mRNA-1:five_prime_utr;Parent=Msa1390830-mRNA-1
tig0021393	CDS	11325	11399	11325	ID=Msa1390830-mRNA-1:cds;Parent=Msa1390830-mRNA-1
tig0021393	CDS	10932	11099	10932	ID=Msa1390830-mRNA-1:cds;Parent=Msa1390830-mRNA-1
tig0021393	three_prime_UTR	10891	10931	10891	ID=Msa1390830-mRNA-1:three_prime_utr;Parent=Msa1390830-mRNA-1

Gene Sequence	>Msa1390830 ATGTTGCTTAATGAAACAATTGCTCTACCGGAATCCGCCACAGCTCTCTCCATGGAACATGGTTTTGACCTCAAGGATTTCTGCTTTCATGCTGATAAAGAAGTATTAAGGGAACCTCGAGTTGTGAGGGTTGGTTTGATTCAGAACTCTATTGCCCTCCCAACAATTGCGCACTTTATCGAGCAAAAAAAGGCTATCTTTGAGAAAGTAAAGCCCATTCAGAACTCTGTTGTTAACTCATGA
Protein sequence	>Msa1390830 MLLNETIALPESATALSMEHGFDLKDFCFHADKEVLREPRVVRVGLIQNSIALPTIAHFIEQKKAIFEKVKPIQNSVVNS*