AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1126700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1126700 MtrunA17_Chr1g0199571 92.308 91 7 0 9 99 127 217 2.07e-52 171
Msa1126700 MtrunA17_Chr1g0156811 58.427 89 37 0 9 97 127 215 1.10e-32 118
Msa1126700 MtrunA17_Chr4g0037111 56.044 91 40 0 9 99 127 217 1.31e-32 115
Msa1126700 MtrunA17_Chr7g0246311 35.484 93 57 2 8 98 124 215 6.77e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1126700 AT5G04220.3 62.637 91 34 0 9 99 65 155 1.60e-35 126
Msa1126700 AT5G04220.2 62.637 91 34 0 9 99 127 217 2.70e-35 126
Msa1126700 AT2G20990.1 54.639 97 44 0 3 99 121 217 1.19e-32 119
Msa1126700 AT2G20990.3 54.639 97 44 0 3 99 121 217 1.34e-32 119
Msa1126700 AT2G20990.2 57.143 91 39 0 9 99 127 217 1.44e-32 119
Msa1126700 AT1G20080.2 50.515 97 48 0 3 99 121 217 7.24e-31 114
Msa1126700 AT1G20080.3 50.515 97 48 0 3 99 121 217 7.24e-31 114
Msa1126700 AT1G20080.4 50.515 97 48 0 3 99 121 217 7.24e-31 114
Msa1126700 AT1G20080.1 50.515 97 48 0 3 99 121 217 7.24e-31 114
Msa1126700 AT1G20080.5 50.515 97 48 0 3 99 121 217 7.24e-31 114
Msa1126700 AT1G05500.1 32.990 97 64 1 3 98 120 216 1.03e-13 65.5
Msa1126700 AT3G61050.2 36.559 93 56 2 8 98 124 215 1.63e-13 65.1
Msa1126700 AT3G61050.1 36.559 93 56 2 8 98 124 215 1.63e-13 65.1
Msa1126700 AT1G05500.2 40.278 72 42 1 28 98 1 72 5.55e-12 60.5

Find 121 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACAGCCTCATGTGGATTT+TGG 0.130792 7_3:-78253573 Msa1126700:intron
ATAATCATGCTGACAGTTTA+TGG 0.142959 7_3:-78252591 Msa1126700:three_prime_UTR
TATTATTGTTATGGATTATT+AGG 0.158712 7_3:+78252648 None:intergenic
TATTAGGACTGCTTAATTTA+AGG 0.161510 7_3:+78252664 None:intergenic
TAGTGTTGAGATTCCTATTA+AGG 0.189939 7_3:-78254287 Msa1126700:five_prime_UTR
ATGCTGATAAAGAAGTATTA+AGG 0.197682 7_3:-78253158 Msa1126700:three_prime_UTR
TCAAAGTTGAGAGGCTAAAT+TGG 0.241187 7_3:+78254663 None:intergenic
GATATTCCTTCATTTGATTT+AGG 0.246878 7_3:-78252260 Msa1126700:three_prime_UTR
TTATATGGATAATTAAATTA+TGG 0.259071 7_3:+78252132 None:intergenic
TTCTGCAGGTATGAAAGTTT+TGG 0.273201 7_3:-78253991 Msa1126700:intron
AACAATGCCAGTTTAACTTT+TGG 0.280661 7_3:+78254249 None:intergenic
TTTGGAGCAACACCCTTAAT+AGG 0.284160 7_3:+78254274 None:intergenic
ACTCTCAATTGGAAGTTATA+TGG 0.289029 7_3:+78252117 None:intergenic
TGCAAGGTCAATACTATGTT+TGG 0.291324 7_3:+78253915 None:intergenic
TCCATAACAATAATAGTAAA+AGG 0.311165 7_3:-78252640 Msa1126700:three_prime_UTR
TCTAACTTTCATCACATTCA+TGG 0.315827 7_3:+78254611 None:intergenic
GCTAAGATCAGCAAAATTTC+AGG 0.326164 7_3:-78254196 Msa1126700:intron
ATTATTGAAGGATAGTTAAC+TGG 0.331648 7_3:-78252919 Msa1126700:three_prime_UTR
GAGAGAGCTGTGGCGGATTC+CGG 0.340791 7_3:+78253444 None:intergenic
GGAAACAAATGAAAAGGAAT+TGG 0.343840 7_3:-78253970 Msa1126700:CDS
TATTCTGCAATGGTTGAATC+AGG 0.347023 7_3:+78252201 None:intergenic
CCAAAATCCAAAAGTTAAAC+TGG 0.349476 7_3:-78254256 Msa1126700:five_prime_UTR
GCAGATAAGTTTAAGACCTT+TGG 0.357838 7_3:-78253751 Msa1126700:CDS
ATGGTTGATTTGGTGTTGTT+TGG 0.367363 7_3:-78251726 Msa1126700:three_prime_UTR
TCGATAAAGTGCGCAATTGT+TGG 0.373558 7_3:+78253087 None:intergenic
GTTTATGGGAGCATGAATTA+TGG 0.389437 7_3:-78252023 Msa1126700:three_prime_UTR
AAAAGTTAAACTGGCATTGT+TGG 0.398909 7_3:-78254247 Msa1126700:five_prime_UTR
AGTGTTGAGATTCCTATTAA+GGG 0.409795 7_3:-78254286 Msa1126700:five_prime_UTR
CGATCCATATGCTGCGATCT+TGG 0.412458 7_3:-78251966 Msa1126700:three_prime_UTR
AAAGATTGTGGTGTCTTTGA+TGG 0.416050 7_3:-78253706 Msa1126700:CDS
CTTCATAACCACATTATTGA+AGG 0.418430 7_3:-78252931 Msa1126700:three_prime_UTR
GTGTAGCGGAGGCAGGGAGG+TGG 0.422043 7_3:-78254775 Msa1126700:intron
AGACATTGGGATGATGGGTT+TGG 0.424062 7_3:-78254686 Msa1126700:five_prime_UTR
TGCTGATAAAGAAGTATTAA+GGG 0.432567 7_3:-78253157 Msa1126700:three_prime_UTR
TATTGCATTCCTTGCAATGA+TGG 0.437005 7_3:+78254709 None:intergenic
GCAATAAGACATTGGGATGA+TGG 0.439230 7_3:-78254692 Msa1126700:five_prime_UTR
TTGCTAACTATAGAAAGAAA+TGG 0.440469 7_3:-78254569 Msa1126700:intron
AAACTTAGGTCTGGATTTGA+AGG 0.442436 7_3:+78251881 None:intergenic
GAGAGTGATGCTTTCTAAGA+TGG 0.442565 7_3:-78252524 Msa1126700:three_prime_UTR
ATAACCAAGATCGCAGCATA+TGG 0.443061 7_3:+78251962 None:intergenic
GCAAGGTCAATACTATGTTT+GGG 0.443699 7_3:+78253916 None:intergenic
CGATAAAGTGCGCAATTGTT+GGG 0.445360 7_3:+78253088 None:intergenic
TTGTTGTGGTCAGGTGCAGC+AGG 0.446623 7_3:-78251814 Msa1126700:three_prime_UTR
ATGAGTGAATACATGTCAAA+TGG 0.450120 7_3:+78255361 None:intergenic
ATGTCTGGTGTAGCGGAGGC+AGG 0.450530 7_3:-78254782 Msa1126700:intron
TAATGAAACAATTGCTCTAC+CGG 0.451479 7_3:-78253463 Msa1126700:three_prime_UTR
CACTAACCACGCTGCAATCA+TGG 0.461908 7_3:+78251911 None:intergenic
TCATGAAACAAGTCATCAAA+TGG 0.463122 7_3:-78253947 Msa1126700:CDS
ATGTATCATAAGCATATTGT+TGG 0.476359 7_3:-78252557 Msa1126700:three_prime_UTR
ATCCATTGGTCTTCTTGCAT+AGG 0.483976 7_3:+78252303 None:intergenic
TACAGAATTTAGAGTCGTAA+AGG 0.487741 7_3:+78252439 None:intergenic
CAAGGAATGCAATAAGACAT+TGG 0.491395 7_3:-78254700 Msa1126700:five_prime_UTR
TAGGTGGTGTTGTTGTGGTC+AGG 0.491423 7_3:-78251823 Msa1126700:three_prime_UTR
ACGAAAGAAGTTGCATCCAT+TGG 0.491832 7_3:+78252289 None:intergenic
AAGGAATGCAATAAGACATT+GGG 0.493762 7_3:-78254699 Msa1126700:five_prime_UTR
TTAACAACAGAGTTCTGAAT+GGG 0.497423 7_3:+78253033 None:intergenic
TGTAGCGGAGGCAGGGAGGT+GGG 0.498715 7_3:-78254774 Msa1126700:intron
CAATAAGACATTGGGATGAT+GGG 0.504343 7_3:-78254691 Msa1126700:five_prime_UTR
GAACCTCGAGTTGTGAGGAT+TGG 0.504458 7_3:-78253135 Msa1126700:three_prime_UTR
TTTGGGTTGGCGATATCTGG+AGG 0.509662 7_3:-78253555 Msa1126700:CDS
AGACGAAGGAAACTTAGGTC+TGG 0.510333 7_3:+78251872 None:intergenic
TGATGCTAGACCCTTCATAC+AGG 0.519583 7_3:+78252992 None:intergenic
AGAGTGATGCTTTCTAAGAT+GGG 0.525015 7_3:-78252523 Msa1126700:three_prime_UTR
ACCACAATCTTTGCAAAACA+TGG 0.526489 7_3:+78253717 None:intergenic
TGACTACCTAAATCAAATGA+AGG 0.528716 7_3:+78252254 None:intergenic
GCTACAGACGAAGGAAACTT+AGG 0.531826 7_3:+78251867 None:intergenic
GCTAGACCCTTCATACAGGC+TGG 0.534194 7_3:+78252996 None:intergenic
GTCTATGAAGATCAAAGTGC+AGG 0.534460 7_3:-78253886 Msa1126700:intron
AGCACATTGTTATTCTGCAA+TGG 0.534464 7_3:+78252191 None:intergenic
TTGGGTTGGCGATATCTGGA+GGG 0.536736 7_3:-78253554 Msa1126700:CDS
GTATGAAGGGTCTAGCATCA+TGG 0.543228 7_3:-78252989 Msa1126700:three_prime_UTR
ATCCGCCACAGCTCTCTCCA+TGG 0.543560 7_3:-78253439 Msa1126700:three_prime_UTR
AACAGCTATAAGTAGATCCT+CGG 0.546715 7_3:+78252343 None:intergenic
TATGAAGGGTCTAGCATCAT+GGG 0.551384 7_3:-78252988 Msa1126700:three_prime_UTR
ATGTCTTCCAGCCTGTATGA+AGG 0.554066 7_3:-78253003 Msa1126700:three_prime_UTR
TTAACTATCCTTCAATAATG+TGG 0.555394 7_3:+78252923 None:intergenic
TCAATTAATTCAAAGTTGAG+AGG 0.557443 7_3:+78254654 None:intergenic
AATTATGGTCCTGGTGTCAA+TGG 0.558417 7_3:-78252008 Msa1126700:three_prime_UTR
GTTAACAACAGAGTTCTGAA+TGG 0.561492 7_3:+78253032 None:intergenic
TGTGGTGTCTTTGATGGAGA+AGG 0.563558 7_3:-78253700 Msa1126700:intron
GTGTGTAGGTGGTGTTGTTG+TGG 0.565056 7_3:-78251828 Msa1126700:three_prime_UTR
TGGAGTGGCTAATATATGAC+AGG 0.565325 7_3:+78252078 None:intergenic
TGTCTTCCAGCCTGTATGAA+GGG 0.565467 7_3:-78253002 Msa1126700:three_prime_UTR
AAACAAGTCATCAAATGGAC+TGG 0.565498 7_3:-78253942 Msa1126700:CDS
TTTCTGGCTCTGTGTGTAGG+TGG 0.566315 7_3:-78251839 Msa1126700:three_prime_UTR
GTTCAATTTACAGCCTCATG+TGG 0.577586 7_3:-78253580 Msa1126700:intron
TGTCTGGTGTAGCGGAGGCA+GGG 0.584181 7_3:-78254781 Msa1126700:intron
GAGGTCAGAACCATGTTCCA+TGG 0.585867 7_3:+78253422 None:intergenic
AGCCTATGCAAGAAGACCAA+TGG 0.586752 7_3:-78252305 Msa1126700:three_prime_UTR
GGAACATGGTTCTGACCTCA+AGG 0.587816 7_3:-78253418 Msa1126700:intron
TTGCAGCGTGGTTAGTGTGT+TGG 0.592356 7_3:-78251905 Msa1126700:three_prime_UTR
TTCCATGGAGAGAGCTGTGG+CGG 0.594000 7_3:+78253437 None:intergenic
GGGAGCATGAATTATGGTCC+TGG 0.596278 7_3:-78252017 Msa1126700:three_prime_UTR
TCTTTCTATAGTTAGCAAAG+TGG 0.597790 7_3:+78254574 None:intergenic
ACAGCTCTCTCCATGGAACA+TGG 0.598176 7_3:-78253432 Msa1126700:three_prime_UTR
GGATGCAACTTCTTTCGTCA+AGG 0.598987 7_3:-78252284 Msa1126700:three_prime_UTR
TTTGCATGATTTGGCCTCCG+AGG 0.600110 7_3:-78252360 Msa1126700:three_prime_UTR
CTGGTGTAGCGGAGGCAGGG+AGG 0.603912 7_3:-78254778 Msa1126700:intron
AAAGTGCGCAATTGTTGGGA+GGG 0.605427 7_3:+78253092 None:intergenic
AACTTAGGTCTGGATTTGAA+GGG 0.615955 7_3:+78251882 None:intergenic
AAACCAATCCTCACAACTCG+AGG 0.620062 7_3:+78253132 None:intergenic
CTACAATATAATCATAATCG+CGG 0.620252 7_3:+78253515 None:intergenic
CTGGTGTCAATGGTAGAAGT+TGG 0.621412 7_3:-78251998 Msa1126700:three_prime_UTR
TAAAGTGCGCAATTGTTGGG+AGG 0.630263 7_3:+78253091 None:intergenic
TCATCAACATTAGCCTCACA+AGG 0.630986 7_3:-78255261 Msa1126700:intron
TAAGGGAACCTCGAGTTGTG+AGG 0.631582 7_3:-78253140 Msa1126700:three_prime_UTR
AAAGGTCTTAAACTTATCTG+CGG 0.650638 7_3:+78253753 None:intergenic
CAACTTCTACCATTGACACC+AGG 0.652303 7_3:+78251999 None:intergenic
TGGGTTGGCGATATCTGGAG+GGG 0.654158 7_3:-78253553 Msa1126700:CDS
CTTCATAGACAACACATGCA+AGG 0.656238 7_3:+78253899 None:intergenic
TCTACTGCACCATCATTGCA+AGG 0.665556 7_3:-78254718 Msa1126700:five_prime_UTR
ATGTTCCATGGAGAGAGCTG+TGG 0.669038 7_3:+78253434 None:intergenic
AATTAAATTATGGCAAAGAG+CGG 0.674865 7_3:+78252142 None:intergenic
GCCAACCCAAAATCCACATG+AGG 0.676621 7_3:+78253567 None:intergenic
CATGGAAGTGTAGACACCAA+AGG 0.678495 7_3:+78253735 None:intergenic
GTGAACATAAGCGAAATGCA+AGG 0.684278 7_3:-78254220 Msa1126700:exon
CTACCACCACTAAAACAGCA+TGG 0.690939 7_3:+78251791 None:intergenic
GAAGGGTCTAGCATCATGGG+AGG 0.706115 7_3:-78252985 Msa1126700:three_prime_UTR
AGCTATAAGTAGATCCTCGG+AGG 0.708824 7_3:+78252346 None:intergenic
ACACGCCGAAGAAAACTGTG+AGG 0.784742 7_3:+78254741 None:intergenic
TTGTTGCCATGATTGCAGCG+TGG 0.786597 7_3:-78251917 Msa1126700:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTATGTTTTGTTTTT+AGG - chr7_3:78252808-78252827 Msa1126700:three_prime_UTR 10.0%
!!! ATTATTATGTTTTGTTTTTA+GGG - chr7_3:78252809-78252828 Msa1126700:three_prime_UTR 10.0%
!!! TATTATTATGTTTTGTTTTT+AGG - chr7_3:78252808-78252827 Msa1126700:three_prime_UTR 10.0%
!!! ATTATTATGTTTTGTTTTTA+GGG - chr7_3:78252809-78252828 Msa1126700:three_prime_UTR 10.0%
!! AATTATTTATCAAATAAGAA+GGG + chr7_3:78253617-78253636 None:intergenic 10.0%
!! TAATTATTTATCAAATAAGA+AGG + chr7_3:78253618-78253637 None:intergenic 10.0%
!!! ATTTTAAGTTTATTTATTTC+AGG - chr7_3:78254026-78254045 Msa1126700:intron 10.0%
!! TTATATGGATAATTAAATTA+TGG + chr7_3:78255090-78255109 None:intergenic 10.0%
!! GTTAAATAAAAAAAAGTTTA+TGG - chr7_3:78255181-78255200 Msa1126700:intron 10.0%
!! AACATAATAATAAGAATGAT+TGG + chr7_3:78252883-78252902 None:intergenic 15.0%
!!! TATTATTGTTATGGATTATT+AGG + chr7_3:78254574-78254593 None:intergenic 15.0%
!! AAAAATAAACACAAGAAAAC+AGG + chr7_3:78251747-78251766 None:intergenic 20.0%
!! AAACAAATCAAACACAAATA+AGG + chr7_3:78252034-78252053 None:intergenic 20.0%
!!! GGATCATTTAAAACATATTA+GGG + chr7_3:78252128-78252147 None:intergenic 20.0%
!!! TGGATCATTTAAAACATATT+AGG + chr7_3:78252129-78252148 None:intergenic 20.0%
!! ATTTGAAGAATGACAAATAT+GGG + chr7_3:78252155-78252174 None:intergenic 20.0%
!! TATTTGAAGAATGACAAATA+TGG + chr7_3:78252156-78252175 None:intergenic 20.0%
!! ATATAATTTACCATGTTGTT+TGG - chr7_3:78252242-78252261 Msa1126700:three_prime_UTR 20.0%
!!! TAGCTTATGAAATATGATTT+TGG - chr7_3:78252316-78252335 Msa1126700:three_prime_UTR 20.0%
!!! ACAAAATGATTTTGTGATAA+TGG - chr7_3:78252700-78252719 Msa1126700:three_prime_UTR 20.0%
!! TTTATCTTGTGATATCATTA+GGG + chr7_3:78253848-78253867 None:intergenic 20.0%
!!! TTTTATCTTGTGATATCATT+AGG + chr7_3:78253849-78253868 None:intergenic 20.0%
!! TAATGATATCACAAGATAAA+AGG - chr7_3:78253848-78253867 Msa1126700:intron 20.0%
!!! GTTATTTAAGCTATAACTTT+TGG - chr7_3:78253992-78254011 Msa1126700:intron 20.0%
!! TGTTTACTTAAAGAAAAAAC+GGG + chr7_3:78254343-78254362 None:intergenic 20.0%
!! GTGTTTACTTAAAGAAAAAA+CGG + chr7_3:78254344-78254363 None:intergenic 20.0%
!!! TTTTTCTTTAAGTAAACACA+TGG - chr7_3:78254345-78254364 Msa1126700:intron 20.0%
!! ATAACCAAAGAAAAAAAAGA+AGG - chr7_3:78254527-78254546 Msa1126700:intron 20.0%
!!! AATACCTTCTTTTTTTTCTT+TGG + chr7_3:78254534-78254553 None:intergenic 20.0%
!! TCCATAACAATAATAGTAAA+AGG - chr7_3:78254579-78254598 Msa1126700:five_prime_UTR 20.0%
!!! ACCTTTTACTATTATTGTTA+TGG + chr7_3:78254583-78254602 None:intergenic 20.0%
!!! TGTGTTTTATTTCTGTTTTA+TGG - chr7_3:78255474-78255493 Msa1126700:five_prime_UTR 20.0%
! ACATATTAGGGTGATAAATT+TGG + chr7_3:78252116-78252135 None:intergenic 25.0%
!!! TTATATTTTGAACTCTAACC+TGG + chr7_3:78252228-78252247 None:intergenic 25.0%
!! AGTTATTGTGTTTTGTCATT+TGG - chr7_3:78252339-78252358 Msa1126700:three_prime_UTR 25.0%
!! TTTCTTCAAGATTTTGTGTT+TGG - chr7_3:78252380-78252399 Msa1126700:three_prime_UTR 25.0%
! TATGAGATTGAATTTATGTC+TGG - chr7_3:78252422-78252441 Msa1126700:three_prime_UTR 25.0%
! TCAATTAATTCAAAGTTGAG+AGG + chr7_3:78252568-78252587 None:intergenic 25.0%
! TTGCTAACTATAGAAAGAAA+TGG - chr7_3:78252650-78252669 Msa1126700:three_prime_UTR 25.0%
!!! TAAGTGTCATTTAGTGATAT+TGG - chr7_3:78252736-78252755 Msa1126700:three_prime_UTR 25.0%
!!! AAGTGTCATTTAGTGATATT+GGG - chr7_3:78252737-78252756 Msa1126700:three_prime_UTR 25.0%
! TGTAAAGCTTTCAAAATACA+AGG - chr7_3:78253064-78253083 Msa1126700:three_prime_UTR 25.0%
! TAATCCTCTCTTTCATTTAT+AGG - chr7_3:78253167-78253186 Msa1126700:three_prime_UTR 25.0%
! AATCCTCTCTTTCATTTATA+GGG - chr7_3:78253168-78253187 Msa1126700:three_prime_UTR 25.0%
!!! AGTTTTGGAAACAAATGAAA+AGG - chr7_3:78253243-78253262 Msa1126700:intron 25.0%
!! TATTTTCTAATTTTCGGTGA+GGG + chr7_3:78253372-78253391 None:intergenic 25.0%
!! TTATTTTCTAATTTTCGGTG+AGG + chr7_3:78253373-78253392 None:intergenic 25.0%
!!! TGATTTTGATTTTTCTCAGT+TGG - chr7_3:78253420-78253439 Msa1126700:three_prime_UTR 25.0%
! TTTCTTCTAATTTCACTTCT+TGG - chr7_3:78253558-78253577 Msa1126700:CDS 25.0%
! TTCTTCTAATTTCACTTCTT+GGG - chr7_3:78253559-78253578 Msa1126700:CDS 25.0%
! CTACAATATAATCATAATCG+CGG + chr7_3:78253707-78253726 None:intergenic 25.0%
!!! ATGCTGATAAAGAAGTATTA+AGG - chr7_3:78254061-78254080 Msa1126700:intron 25.0%
!!! TGCTGATAAAGAAGTATTAA+GGG - chr7_3:78254062-78254081 Msa1126700:intron 25.0%
! TTAACTATCCTTCAATAATG+TGG + chr7_3:78254299-78254318 None:intergenic 25.0%
! ATTATTGAAGGATAGTTAAC+TGG - chr7_3:78254300-78254319 Msa1126700:five_prime_UTR 25.0%
! TATTAGGACTGCTTAATTTA+AGG + chr7_3:78254558-78254577 None:intergenic 25.0%
! ATGTATCATAAGCATATTGT+TGG - chr7_3:78254662-78254681 Msa1126700:five_prime_UTR 25.0%
!! GATATTCCTTCATTTGATTT+AGG - chr7_3:78254959-78254978 Msa1126700:intron 25.0%
! AATTAAATTATGGCAAAGAG+CGG + chr7_3:78255080-78255099 None:intergenic 25.0%
! AGCTTAAAAAAACTCTCAAT+TGG + chr7_3:78255116-78255135 None:intergenic 25.0%
!! TTTCTGTTTTATGGTTGATT+TGG - chr7_3:78255483-78255502 Msa1126700:five_prime_UTR 25.0%
ATGAGTGAATACATGTCAAA+TGG + chr7_3:78251861-78251880 None:intergenic 30.0%
TAAAAACATCAAACCTTGTG+AGG + chr7_3:78251974-78251993 None:intergenic 30.0%
! AAGATTTTGTGTTTGGTCAT+TGG - chr7_3:78252387-78252406 Msa1126700:three_prime_UTR 30.0%
AAGGAATGCAATAAGACATT+GGG - chr7_3:78252520-78252539 Msa1126700:three_prime_UTR 30.0%
TCTAACTTTCATCACATTCA+TGG + chr7_3:78252611-78252630 None:intergenic 30.0%
TCTTTCTATAGTTAGCAAAG+TGG + chr7_3:78252648-78252667 None:intergenic 30.0%
AATGGTACGATAGATAAGTA+AGG - chr7_3:78252668-78252687 Msa1126700:three_prime_UTR 30.0%
ATGGTACGATAGATAAGTAA+GGG - chr7_3:78252669-78252688 Msa1126700:three_prime_UTR 30.0%
!! AGTGTCATTTAGTGATATTG+GGG - chr7_3:78252738-78252757 Msa1126700:three_prime_UTR 30.0%
AAGAATGAAAGTGATGATCA+TGG + chr7_3:78252789-78252808 None:intergenic 30.0%
TAGTGTTGTGATTCCTATTA+AGG - chr7_3:78252849-78252868 Msa1126700:three_prime_UTR 30.0%
AGTGTTGTGATTCCTATTAA+GGG - chr7_3:78252850-78252869 Msa1126700:three_prime_UTR 30.0%
! TAGTGTTGAGATTCCTATTA+AGG - chr7_3:78252932-78252951 Msa1126700:three_prime_UTR 30.0%
! AGTGTTGAGATTCCTATTAA+GGG - chr7_3:78252933-78252952 Msa1126700:three_prime_UTR 30.0%
!! CCAGTTTAACTTTTGGATTT+TGG + chr7_3:78252966-78252985 None:intergenic 30.0%
CCAAAATCCAAAAGTTAAAC+TGG - chr7_3:78252963-78252982 Msa1126700:three_prime_UTR 30.0%
!! AACAATGCCAGTTTAACTTT+TGG + chr7_3:78252973-78252992 None:intergenic 30.0%
AAAAGTTAAACTGGCATTGT+TGG - chr7_3:78252972-78252991 Msa1126700:three_prime_UTR 30.0%
! CTTTGTTTCTTTGATGTGTT+TGG - chr7_3:78253104-78253123 Msa1126700:three_prime_UTR 30.0%
ATCCTCTCTTTCATTTATAG+GGG - chr7_3:78253169-78253188 Msa1126700:three_prime_UTR 30.0%
GGAAACAAATGAAAAGGAAT+TGG - chr7_3:78253249-78253268 Msa1126700:intron 30.0%
TCATGAAACAAGTCATCAAA+TGG - chr7_3:78253272-78253291 Msa1126700:intron 30.0%
AAAGGTCTTAAACTTATCTG+CGG + chr7_3:78253469-78253488 None:intergenic 30.0%
TAATGAAACAATTGCTCTAC+CGG - chr7_3:78253756-78253775 Msa1126700:CDS 30.0%
!! TTAACAACAGAGTTCTGAAT+GGG + chr7_3:78254189-78254208 None:intergenic 30.0%
CTTCATAACCACATTATTGA+AGG - chr7_3:78254288-78254307 Msa1126700:five_prime_UTR 30.0%
!! ATCTTGTGTCTACTTTTCTT+TGG + chr7_3:78254488-78254507 None:intergenic 30.0%
ATAATCATGCTGACAGTTTA+TGG - chr7_3:78254628-78254647 Msa1126700:five_prime_UTR 30.0%
! TACAGAATTTAGAGTCGTAA+AGG + chr7_3:78254783-78254802 None:intergenic 30.0%
! TTTTGTCTCTTTGCATGATT+TGG - chr7_3:78254850-78254869 Msa1126700:intron 30.0%
TGACTACCTAAATCAAATGA+AGG + chr7_3:78254968-78254987 None:intergenic 30.0%
ACTCTCAATTGGAAGTTATA+TGG + chr7_3:78255105-78255124 None:intergenic 30.0%
AACGAAAAAAATGTCTTCTG+TGG + chr7_3:78255164-78255183 None:intergenic 30.0%
!!! CGATCTTGGTTATTTTTACA+AGG - chr7_3:78255267-78255286 Msa1126700:five_prime_UTR 30.0%
TGAAGAATGACAAATATGGG+AGG + chr7_3:78252152-78252171 None:intergenic 35.0%
TTCTTCGATACCAAACAACA+TGG + chr7_3:78252255-78252274 None:intergenic 35.0%
TGAATTTATGTCTGGTGTAG+CGG - chr7_3:78252430-78252449 Msa1126700:three_prime_UTR 35.0%
TATTGCATTCCTTGCAATGA+TGG + chr7_3:78252513-78252532 None:intergenic 35.0%
CAAGGAATGCAATAAGACAT+TGG - chr7_3:78252519-78252538 Msa1126700:three_prime_UTR 35.0%
CAATAAGACATTGGGATGAT+GGG - chr7_3:78252528-78252547 Msa1126700:three_prime_UTR 35.0%
TCAAAGTTGAGAGGCTAAAT+TGG + chr7_3:78252559-78252578 None:intergenic 35.0%
GCTAAGATCAGCAAAATTTC+AGG - chr7_3:78253023-78253042 Msa1126700:three_prime_UTR 35.0%
ACAAAGCAACAAAATCACAG+AGG + chr7_3:78253090-78253109 None:intergenic 35.0%
TACCCCTATAAATGAAAGAG+AGG + chr7_3:78253174-78253193 None:intergenic 35.0%
ATTGTTTGTAGCAATTCTGC+AGG - chr7_3:78253214-78253233 Msa1126700:intron 35.0%
! TTCTGCAGGTATGAAAGTTT+TGG - chr7_3:78253228-78253247 Msa1126700:intron 35.0%
AAACAAGTCATCAAATGGAC+TGG - chr7_3:78253277-78253296 Msa1126700:intron 35.0%
GCAAGGTCAATACTATGTTT+GGG + chr7_3:78253306-78253325 None:intergenic 35.0%
TGCAAGGTCAATACTATGTT+TGG + chr7_3:78253307-78253326 None:intergenic 35.0%
! GCAGATAAGTTTAAGACCTT+TGG - chr7_3:78253468-78253487 Msa1126700:three_prime_UTR 35.0%
! TCCATGTTTTGCAAAGATTG+TGG - chr7_3:78253501-78253520 Msa1126700:three_prime_UTR 35.0%
ACCACAATCTTTGCAAAACA+TGG + chr7_3:78253505-78253524 None:intergenic 35.0%
AAAGATTGTGGTGTCTTTGA+TGG - chr7_3:78253513-78253532 Msa1126700:three_prime_UTR 35.0%
TGAAGTGTATTCATACCTTG+AGG + chr7_3:78253819-78253838 None:intergenic 35.0%
! TCACAAGATAAAAGGTATGC+TGG - chr7_3:78253856-78253875 Msa1126700:intron 35.0%
GCACTTTATCGAGCAAAAAA+AGG - chr7_3:78254143-78254162 Msa1126700:intron 35.0%
!! GTTAACAACAGAGTTCTGAA+TGG + chr7_3:78254190-78254209 None:intergenic 35.0%
! TAACTGGATTTTGCCAAGTT+AGG - chr7_3:78254316-78254335 Msa1126700:intron 35.0%
! AACTGGATTTTGCCAAGTTA+GGG - chr7_3:78254317-78254336 Msa1126700:intron 35.0%
!! AGAGTGATGCTTTCTAAGAT+GGG - chr7_3:78254696-78254715 Msa1126700:five_prime_UTR 35.0%
AACAGCTATAAGTAGATCCT+CGG + chr7_3:78254879-78254898 None:intergenic 35.0%
TATTCTGCAATGGTTGAATC+AGG + chr7_3:78255021-78255040 None:intergenic 35.0%
! AGCACATTGTTATTCTGCAA+TGG + chr7_3:78255031-78255050 None:intergenic 35.0%
AAAAAATGTCTTCTGTGGAG+TGG + chr7_3:78255159-78255178 None:intergenic 35.0%
! GTTTATGGGAGCATGAATTA+TGG - chr7_3:78255196-78255215 Msa1126700:intron 35.0%
AACTTAGGTCTGGATTTGAA+GGG + chr7_3:78255340-78255359 None:intergenic 35.0%
AAACTTAGGTCTGGATTTGA+AGG + chr7_3:78255341-78255360 None:intergenic 35.0%
! TCATCAACATTAGCCTCACA+AGG - chr7_3:78251958-78251977 Msa1126700:three_prime_UTR 40.0%
CTGTATGCTAGATATGGACT+GGG + chr7_3:78252066-78252085 None:intergenic 40.0%
CAGTCCATATCTAGCATACA+GGG - chr7_3:78252065-78252084 Msa1126700:three_prime_UTR 40.0%
CAATCCCTGTATGCTAGATA+TGG + chr7_3:78252072-78252091 None:intergenic 40.0%
AGAATGACAAATATGGGAGG+TGG + chr7_3:78252149-78252168 None:intergenic 40.0%
GCTCTGCAGATTTATTTACC+AGG - chr7_3:78252207-78252226 Msa1126700:three_prime_UTR 40.0%
! GCAATCCTCACAGTTTTCTT+CGG - chr7_3:78252473-78252492 Msa1126700:three_prime_UTR 40.0%
GCAATAAGACATTGGGATGA+TGG - chr7_3:78252527-78252546 Msa1126700:three_prime_UTR 40.0%
GTCATTTAGTGATATTGGGG+TGG - chr7_3:78252741-78252760 Msa1126700:three_prime_UTR 40.0%
ATTGGAGCAACACCCTTAAT+AGG + chr7_3:78252865-78252884 None:intergenic 40.0%
! TTTGGAGCAACACCCTTAAT+AGG + chr7_3:78252948-78252967 None:intergenic 40.0%
GTGAACATAAGCGAAATGCA+AGG - chr7_3:78252999-78253018 Msa1126700:three_prime_UTR 40.0%
CTTCATAGACAACACATGCA+AGG + chr7_3:78253323-78253342 None:intergenic 40.0%
GTCTATGAAGATCAAAGTGC+AGG - chr7_3:78253333-78253352 Msa1126700:intron 40.0%
! TTCTAATTTTCGGTGAGGGT+AGG + chr7_3:78253368-78253387 None:intergenic 40.0%
TGTCTTTGATGGAGAAGGTA+AGG - chr7_3:78253524-78253543 Msa1126700:exon 40.0%
GTCTTTGATGGAGAAGGTAA+GGG - chr7_3:78253525-78253544 Msa1126700:exon 40.0%
GTTCAATTTACAGCCTCATG+TGG - chr7_3:78253639-78253658 Msa1126700:intron 40.0%
! TTACAGCCTCATGTGGATTT+TGG - chr7_3:78253646-78253665 Msa1126700:intron 40.0%
! TACAGCCTCATGTGGATTTT+GGG - chr7_3:78253647-78253666 Msa1126700:intron 40.0%
GCATACACTCCATCAGTTTA+AGG + chr7_3:78253916-78253935 None:intergenic 40.0%
ACAGACAAGTTTCAATGCTG+AGG + chr7_3:78253974-78253993 None:intergenic 40.0%
CGATAAAGTGCGCAATTGTT+GGG + chr7_3:78254134-78254153 None:intergenic 40.0%
TCGATAAAGTGCGCAATTGT+TGG + chr7_3:78254135-78254154 None:intergenic 40.0%
TATGAAGGGTCTAGCATCAT+GGG - chr7_3:78254231-78254250 Msa1126700:five_prime_UTR 40.0%
! GAGAGTGATGCTTTCTAAGA+TGG - chr7_3:78254695-78254714 Msa1126700:five_prime_UTR 40.0%
!! ATCCATTGGTCTTCTTGCAT+AGG + chr7_3:78254919-78254938 None:intergenic 40.0%
ACGAAAGAAGTTGCATCCAT+TGG + chr7_3:78254933-78254952 None:intergenic 40.0%
TGGAGTGGCTAATATATGAC+AGG + chr7_3:78255144-78255163 None:intergenic 40.0%
! AATTATGGTCCTGGTGTCAA+TGG - chr7_3:78255211-78255230 Msa1126700:intron 40.0%
ATAACCAAGATCGCAGCATA+TGG + chr7_3:78255260-78255279 None:intergenic 40.0%
GAAAAAGTTGCTACAGACGA+AGG + chr7_3:78255364-78255383 None:intergenic 40.0%
!! TCGTCTGTAGCAACTTTTTC+TGG - chr7_3:78255364-78255383 Msa1126700:five_prime_UTR 40.0%
CCTGTATGCTAGATATGGAC+TGG + chr7_3:78252067-78252086 None:intergenic 45.0%
CCAGTCCATATCTAGCATAC+AGG - chr7_3:78252064-78252083 Msa1126700:three_prime_UTR 45.0%
ATTTATGTCTGGTGTAGCGG+AGG - chr7_3:78252433-78252452 Msa1126700:three_prime_UTR 45.0%
TCTACTGCACCATCATTGCA+AGG - chr7_3:78252501-78252520 Msa1126700:three_prime_UTR 45.0%
!! AGACATTGGGATGATGGGTT+TGG - chr7_3:78252533-78252552 Msa1126700:three_prime_UTR 45.0%
! CATGGAAGTGTAGACACCAA+AGG + chr7_3:78253487-78253506 None:intergenic 45.0%
TGTGGTGTCTTTGATGGAGA+AGG - chr7_3:78253519-78253538 Msa1126700:three_prime_UTR 45.0%
!!! GATTTTGGGTTGGCGATATC+TGG - chr7_3:78253661-78253680 Msa1126700:intron 45.0%
!! GCTGTGTTGCTTCCTTAAAC+TGG - chr7_3:78253888-78253907 Msa1126700:CDS 45.0%
! CAGTTTAAGGCGCCAGTTTA+AGG + chr7_3:78253903-78253922 None:intergenic 45.0%
AAACTGGCGCCTTAAACTGA+TGG - chr7_3:78253904-78253923 Msa1126700:CDS 45.0%
TGCTCGATTTCTCGACAACA+CGG - chr7_3:78253933-78253952 Msa1126700:CDS 45.0%
AAACCAATCCTCACAACTCG+AGG + chr7_3:78254090-78254109 None:intergenic 45.0%
AAAGTGCGCAATTGTTGGGA+GGG + chr7_3:78254130-78254149 None:intergenic 45.0%
TAAAGTGCGCAATTGTTGGG+AGG + chr7_3:78254131-78254150 None:intergenic 45.0%
ATGTCTTCCAGCCTGTATGA+AGG - chr7_3:78254216-78254235 Msa1126700:exon 45.0%
TGTCTTCCAGCCTGTATGAA+GGG - chr7_3:78254217-78254236 Msa1126700:exon 45.0%
TGATGCTAGACCCTTCATAC+AGG + chr7_3:78254230-78254249 None:intergenic 45.0%
GTATGAAGGGTCTAGCATCA+TGG - chr7_3:78254230-78254249 Msa1126700:five_prime_UTR 45.0%
AGAAAAAACGGGCCCTAACT+TGG + chr7_3:78254332-78254351 None:intergenic 45.0%
AGCTATAAGTAGATCCTCGG+AGG + chr7_3:78254876-78254895 None:intergenic 45.0%
AGCCTATGCAAGAAGACCAA+TGG - chr7_3:78254914-78254933 Msa1126700:intron 45.0%
GGATGCAACTTCTTTCGTCA+AGG - chr7_3:78254935-78254954 Msa1126700:intron 45.0%
CAACTTCTACCATTGACACC+AGG + chr7_3:78255223-78255242 None:intergenic 45.0%
! CTGGTGTCAATGGTAGAAGT+TGG - chr7_3:78255221-78255240 Msa1126700:intron 45.0%
AGACGAAGGAAACTTAGGTC+TGG + chr7_3:78255350-78255369 None:intergenic 45.0%
GCTACAGACGAAGGAAACTT+AGG + chr7_3:78255355-78255374 None:intergenic 45.0%
!!! CTTTTTCTGGCTCTGTGTGT+AGG - chr7_3:78255377-78255396 Msa1126700:five_prime_UTR 45.0%
CTACCACCACTAAAACAGCA+TGG + chr7_3:78255431-78255450 None:intergenic 45.0%
!!! AATTATTATTTTCTAATTTT+CGG + chr7_3:78253378-78253397 None:intergenic 5.0%
!! TTAAATAAAAAAAAGTTTAT+GGG - chr7_3:78255182-78255201 Msa1126700:intron 5.0%
ACACGCCGAAGAAAACTGTG+AGG + chr7_3:78252481-78252500 None:intergenic 50.0%
!! GCCTCATGTGGATTTTGGGT+TGG - chr7_3:78253651-78253670 Msa1126700:intron 50.0%
GCCAACCCAAAATCCACATG+AGG + chr7_3:78253655-78253674 None:intergenic 50.0%
!! TTTGGGTTGGCGATATCTGG+AGG - chr7_3:78253664-78253683 Msa1126700:intron 50.0%
!! TTGGGTTGGCGATATCTGGA+GGG - chr7_3:78253665-78253684 Msa1126700:intron 50.0%
ATGTTCCATGGAGAGAGCTG+TGG + chr7_3:78253788-78253807 None:intergenic 50.0%
ACAGCTCTCTCCATGGAACA+TGG - chr7_3:78253787-78253806 Msa1126700:intron 50.0%
GAGGTCAGAACCATGTTCCA+TGG + chr7_3:78253800-78253819 None:intergenic 50.0%
!! GGAACATGGTTCTGACCTCA+AGG - chr7_3:78253801-78253820 Msa1126700:intron 50.0%
! TAAGGGAACCTCGAGTTGTG+AGG - chr7_3:78254079-78254098 Msa1126700:intron 50.0%
! GAACCTCGAGTTGTGAGGAT+TGG - chr7_3:78254084-78254103 Msa1126700:intron 50.0%
! TTTGCATGATTTGGCCTCCG+AGG - chr7_3:78254859-78254878 Msa1126700:intron 50.0%
! GGGAGCATGAATTATGGTCC+TGG - chr7_3:78255202-78255221 Msa1126700:intron 50.0%
CGATCCATATGCTGCGATCT+TGG - chr7_3:78255253-78255272 Msa1126700:intron 50.0%
TTGTTGCCATGATTGCAGCG+TGG - chr7_3:78255302-78255321 Msa1126700:five_prime_UTR 50.0%
CACTAACCACGCTGCAATCA+TGG + chr7_3:78255311-78255330 None:intergenic 50.0%
TTGCAGCGTGGTTAGTGTGT+TGG - chr7_3:78255314-78255333 Msa1126700:five_prime_UTR 50.0%
!! TTTCTGGCTCTGTGTGTAGG+TGG - chr7_3:78255380-78255399 Msa1126700:five_prime_UTR 50.0%
!! GTGTGTAGGTGGTGTTGTTG+TGG - chr7_3:78255391-78255410 Msa1126700:five_prime_UTR 50.0%
!! TAGGTGGTGTTGTTGTGGTC+AGG - chr7_3:78255396-78255415 Msa1126700:five_prime_UTR 50.0%
!!! AGCAGGCCATGCTGTTTTAG+TGG - chr7_3:78255422-78255441 Msa1126700:five_prime_UTR 50.0%
!!! AGGCCATGCTGTTTTAGTGG+TGG - chr7_3:78255425-78255444 Msa1126700:five_prime_UTR 50.0%
!! TGGGTTGGCGATATCTGGAG+GGG - chr7_3:78253666-78253685 Msa1126700:intron 55.0%
TTCCATGGAGAGAGCTGTGG+CGG + chr7_3:78253785-78253804 None:intergenic 55.0%
GCTAGACCCTTCATACAGGC+TGG + chr7_3:78254226-78254245 None:intergenic 55.0%
GAAGGGTCTAGCATCATGGG+AGG - chr7_3:78254234-78254253 Msa1126700:five_prime_UTR 55.0%
TTGTTGTGGTCAGGTGCAGC+AGG - chr7_3:78255405-78255424 Msa1126700:five_prime_UTR 55.0%
! ATGTCTGGTGTAGCGGAGGC+AGG - chr7_3:78252437-78252456 Msa1126700:three_prime_UTR 60.0%
! TGTCTGGTGTAGCGGAGGCA+GGG - chr7_3:78252438-78252457 Msa1126700:three_prime_UTR 60.0%
!! GAGAGAGCTGTGGCGGATTC+CGG + chr7_3:78253778-78253797 None:intergenic 60.0%
ATCCGCCACAGCTCTCTCCA+TGG - chr7_3:78253780-78253799 Msa1126700:CDS 60.0%
! TGTAGCGGAGGCAGGGAGGT+GGG - chr7_3:78252445-78252464 Msa1126700:three_prime_UTR 65.0%
! CTGGTGTAGCGGAGGCAGGG+AGG - chr7_3:78252441-78252460 Msa1126700:three_prime_UTR 70.0%
! GTGTAGCGGAGGCAGGGAGG+TGG - chr7_3:78252444-78252463 Msa1126700:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 78251726 78255515 78251726 ID=Msa1126700;Name=Msa1126700
chr7_3 mRNA 78251726 78255515 78251726 ID=Msa1126700-mRNA-1;Parent=Msa1126700;Name=Msa1126700-mRNA-1;_AED=0.01;_eAED=0.01;_QI=577|1|1|1|0.66|0.42|7|1591|99
chr7_3 exon 78251726 78253193 78251726 ID=Msa1126700-mRNA-1:exon:21793;Parent=Msa1126700-mRNA-1
chr7_3 exon 78253419 78253589 78253419 ID=Msa1126700-mRNA-1:exon:21792;Parent=Msa1126700-mRNA-1
chr7_3 exon 78253701 78253802 78253701 ID=Msa1126700-mRNA-1:exon:21791;Parent=Msa1126700-mRNA-1
chr7_3 exon 78253887 78254005 78253887 ID=Msa1126700-mRNA-1:exon:21790;Parent=Msa1126700-mRNA-1
chr7_3 exon 78254197 78254328 78254197 ID=Msa1126700-mRNA-1:exon:21789;Parent=Msa1126700-mRNA-1
chr7_3 exon 78254570 78254791 78254570 ID=Msa1126700-mRNA-1:exon:21788;Parent=Msa1126700-mRNA-1
chr7_3 exon 78255262 78255515 78255262 ID=Msa1126700-mRNA-1:exon:21787;Parent=Msa1126700-mRNA-1
chr7_3 five_prime_UTR 78255262 78255515 78255262 ID=Msa1126700-mRNA-1:five_prime_utr;Parent=Msa1126700-mRNA-1
chr7_3 five_prime_UTR 78254570 78254791 78254570 ID=Msa1126700-mRNA-1:five_prime_utr;Parent=Msa1126700-mRNA-1
chr7_3 five_prime_UTR 78254228 78254328 78254228 ID=Msa1126700-mRNA-1:five_prime_utr;Parent=Msa1126700-mRNA-1
chr7_3 CDS 78254197 78254227 78254197 ID=Msa1126700-mRNA-1:cds;Parent=Msa1126700-mRNA-1
chr7_3 CDS 78253887 78254005 78253887 ID=Msa1126700-mRNA-1:cds;Parent=Msa1126700-mRNA-1
chr7_3 CDS 78253701 78253802 78253701 ID=Msa1126700-mRNA-1:cds;Parent=Msa1126700-mRNA-1
chr7_3 CDS 78253542 78253589 78253542 ID=Msa1126700-mRNA-1:cds;Parent=Msa1126700-mRNA-1
chr7_3 three_prime_UTR 78253419 78253541 78253419 ID=Msa1126700-mRNA-1:three_prime_utr;Parent=Msa1126700-mRNA-1
chr7_3 three_prime_UTR 78251726 78253193 78251726 ID=Msa1126700-mRNA-1:three_prime_utr;Parent=Msa1126700-mRNA-1
Gene Sequence

>Msa1126700

ATGCAAGGTGCTAAGATCAGCAAAATTTCAGGTATGAAAGTTTTGGAAACAAATGAAAAGGAATTGGTCATGAAACAAGTCATCAAATGGACTGGCAACCCAAACATAGTATTGACCTTGCATGTGTTGTCTATGAAGATCAAAGTGCAGTTGGTAGACTTACAAGTTTTTGCGACGCCGCAGATAAGTTTAAGACCTTTGGTGTCTACACTTCCATGTTTTGCAAAGATTGTGGTGTCTTTGATGGAGAAGCCTCATGTGGATTTTGGGTTGGCGATATCTGGAGGGGACATCATATAG
Protein sequence

>Msa1126700

MQGAKISKISGMKVLETNEKELVMKQVIKWTGNPNIVLTLHVLSMKIKVQLVDLQVFATPQISLRPLVSTLPCFAKIVVSLMEKPHVDFGLAISGGDII*