AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1265150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265150 MtrunA17_Chr1g0199571 92.308 78 6 0 9 86 127 204 3.11e-42 147
Msa1265150 MtrunA17_Chr4g0049851 72.165 97 20 1 84 173 38 134 1.36e-40 141
Msa1265150 MtrunA17_Chr4g0049861 58.763 97 33 1 84 173 36 132 3.91e-32 119
Msa1265150 MtrunA17_Chr1g0156811 48.515 101 51 1 3 103 121 220 1.37e-26 104
Msa1265150 MtrunA17_Chr4g0037111 53.571 84 39 0 3 86 121 204 1.74e-25 100
Msa1265150 MtrunA17_Chr7g0246311 32.990 97 63 2 8 103 124 219 7.85e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265150 AT5G04220.3 54.737 95 42 1 9 103 65 158 4.86e-28 108
Msa1265150 AT5G04220.2 54.737 95 42 1 9 103 127 220 5.89e-28 108
Msa1265150 AT2G20990.3 54.762 84 38 0 3 86 121 204 1.86e-25 102
Msa1265150 AT2G20990.1 54.762 84 38 0 3 86 121 204 1.99e-25 102
Msa1265150 AT2G20990.2 54.762 84 38 0 3 86 121 204 2.01e-25 102
Msa1265150 AT1G20080.2 45.545 101 54 1 3 103 121 220 4.91e-25 100
Msa1265150 AT1G20080.3 45.545 101 54 1 3 103 121 220 4.91e-25 100
Msa1265150 AT1G20080.4 45.545 101 54 1 3 103 121 220 4.91e-25 100
Msa1265150 AT1G20080.1 45.545 101 54 1 3 103 121 220 4.91e-25 100
Msa1265150 AT1G20080.5 45.545 101 54 1 3 103 121 220 4.91e-25 100
Msa1265150 AT5G64370.1 55.556 90 33 1 84 166 32 121 4.42e-23 94.7
Msa1265150 AT3G61050.2 32.990 97 63 2 8 103 124 219 4.76e-11 60.8
Msa1265150 AT3G61050.1 32.990 97 63 2 8 103 124 219 4.76e-11 60.8

Find 113 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATCATGCTGACAGTTTC+TGG 0.155569 8_2:+69255661 Msa1265150:three_prime_UTR
TATTATTGTTATGGATTATT+AGG 0.158712 8_2:-69255604 None:intergenic
TATTAGGACTGCTTAATTTA+AGG 0.161510 8_2:-69255588 None:intergenic
TAGTGTTGAGATTCCTATTA+AGG 0.189939 8_2:+69253963 Msa1265150:five_prime_UTR
ATGCTGATAAAGAAGTATTA+AGG 0.197682 8_2:+69255092 Msa1265150:CDS
AACAATGCCACTTTAACTTT+TGG 0.230938 8_2:-69254001 None:intergenic
TCAAAGTTGAGAGGCTAAAT+TGG 0.241187 8_2:-69253587 None:intergenic
GATATTCCTTCATTTGATTT+AGG 0.246878 8_2:+69255992 Msa1265150:three_prime_UTR
TTATATGGATAATTAAATTA+TGG 0.259071 8_2:-69256120 None:intergenic
TTCTGCAGGTATGAAAGTTT+TGG 0.273201 8_2:+69254259 Msa1265150:intron
TTTGGAGCAACACCCTTAAT+AGG 0.284160 8_2:-69253976 None:intergenic
ACTCTCAATTGGAAGTTATA+TGG 0.289029 8_2:-69256135 None:intergenic
TGCAAGGTCAATACTATGTT+TGG 0.291324 8_2:-69254335 None:intergenic
TCCATAACAATAATAGTAAA+AGG 0.311165 8_2:+69255612 Msa1265150:three_prime_UTR
TCTAACTTTCATCACATTCA+TGG 0.315827 8_2:-69253639 None:intergenic
GCTAAGATCAGCAAAATTTC+AGG 0.326164 8_2:+69254054 Msa1265150:CDS
ATTATTGAAGGATAGTTAAC+TGG 0.331648 8_2:+69255331 Msa1265150:three_prime_UTR
GAGAGAGCTGTGGCGGATTC+CGG 0.340791 8_2:-69254806 None:intergenic
GGAAACAAATGAAAAGGAAT+TGG 0.343840 8_2:+69254280 Msa1265150:CDS
TATTCTGCAATGGTTGAATC+AGG 0.347023 8_2:-69256051 None:intergenic
GCAGATAAGTTTAAGACCTT+TGG 0.357838 8_2:+69254499 Msa1265150:CDS
ATGGTTGATTTGGTGTTGTT+TGG 0.358895 8_2:+69256526 Msa1265150:three_prime_UTR
TCGATAAAGTGCGCAATTGT+TGG 0.373558 8_2:-69255163 None:intergenic
GTTTATGGGAGCATGAATTA+TGG 0.389437 8_2:+69256229 Msa1265150:three_prime_UTR
CGATCTATATGCTGCGATCT+TGG 0.403507 8_2:+69256286 Msa1265150:three_prime_UTR
AGTGTTGAGATTCCTATTAA+GGG 0.409795 8_2:+69253964 Msa1265150:five_prime_UTR
AAAGATTGTGGTGTCTTTGA+TGG 0.416050 8_2:+69254544 Msa1265150:CDS
CTTCATAACCACATTATTGA+AGG 0.418430 8_2:+69255319 Msa1265150:three_prime_UTR
GTGTAGCGGAGGCAGGGAGG+TGG 0.422043 8_2:+69253475 Msa1265150:intron
AGACATTGGGATGATGGGTT+TGG 0.424062 8_2:+69253564 Msa1265150:five_prime_UTR
AAAAGTTAAAGTGGCATTGT+TGG 0.426962 8_2:+69254003 Msa1265150:five_prime_UTR
TGCTGATAAAGAAGTATTAA+GGG 0.432567 8_2:+69255093 Msa1265150:CDS
TATTGCATTCCTTGCAATGA+TGG 0.437005 8_2:-69253541 None:intergenic
GCAATAAGACATTGGGATGA+TGG 0.439230 8_2:+69253558 Msa1265150:five_prime_UTR
TTGCTAACTATAGAAAGAAA+TGG 0.440469 8_2:+69253681 Msa1265150:five_prime_UTR
AAACTTAGGTCTGGATTTGA+AGG 0.442436 8_2:-69256371 None:intergenic
GAGAGTGATGCTTTCTAAGA+TGG 0.442565 8_2:+69255728 Msa1265150:three_prime_UTR
GCAAGGTCAATACTATGTTT+GGG 0.443699 8_2:-69254334 None:intergenic
CGATAAAGTGCGCAATTGTT+GGG 0.445360 8_2:-69255162 None:intergenic
TTGTTGTGGTCAGGTGCAGC+AGG 0.446623 8_2:+69256438 Msa1265150:three_prime_UTR
ATGAGTGAATACATGTCAAA+TGG 0.450120 8_2:-69252889 None:intergenic
ATGTCTGGTGTAGCGGAGGC+AGG 0.450530 8_2:+69253468 Msa1265150:intron
TAATGAAACAATTGCTCTAC+CGG 0.451479 8_2:+69254787 Msa1265150:CDS
CACTAACCACGCTGCAATCA+TGG 0.461908 8_2:-69256341 None:intergenic
TCATGAAACAAGTCATCAAA+TGG 0.463122 8_2:+69254303 Msa1265150:CDS
ATGTATCATAAGCATATTGT+TGG 0.476359 8_2:+69255695 Msa1265150:three_prime_UTR
CCAAAATCCAAAAGTTAAAG+TGG 0.476564 8_2:+69253994 Msa1265150:five_prime_UTR
ATCCATTGGTCTTCTTGCAT+AGG 0.483976 8_2:-69255949 None:intergenic
TACAGAATTTAGAGTCGTAA+AGG 0.487741 8_2:-69255813 None:intergenic
CAAGGAATGCAATAAGACAT+TGG 0.491395 8_2:+69253550 Msa1265150:five_prime_UTR
TAGGTGGTGTTGTTGTGGTC+AGG 0.491423 8_2:+69256429 Msa1265150:three_prime_UTR
ACGAAAGAAGTTGCATCCAT+TGG 0.491832 8_2:-69255963 None:intergenic
AAGGAATGCAATAAGACATT+GGG 0.493762 8_2:+69253551 Msa1265150:five_prime_UTR
TTAACAACAGAGTTCTGAAT+GGG 0.497423 8_2:-69255217 None:intergenic
TGTAGCGGAGGCAGGGAGGT+GGG 0.498715 8_2:+69253476 Msa1265150:intron
CAATAAGACATTGGGATGAT+GGG 0.504343 8_2:+69253559 Msa1265150:five_prime_UTR
GCTACAGACGGAGGAAACTT+AGG 0.512242 8_2:-69256385 None:intergenic
AGACGGAGGAAACTTAGGTC+TGG 0.518668 8_2:-69256380 None:intergenic
TGATGCTAGACCCTTCATAC+AGG 0.519583 8_2:-69255258 None:intergenic
AGAGTGATGCTTTCTAAGAT+GGG 0.525015 8_2:+69255729 Msa1265150:three_prime_UTR
ACCACAATCTTTGCAAAACA+TGG 0.526489 8_2:-69254533 None:intergenic
TGACTACCTAAATCAAATGA+AGG 0.528716 8_2:-69255998 None:intergenic
GCTAGACCCTTCATACAGGC+TGG 0.534194 8_2:-69255254 None:intergenic
GTCTATGAAGATCAAAGTGC+AGG 0.534460 8_2:+69254364 Msa1265150:CDS
AGCACATTGTTATTCTGCAA+TGG 0.534464 8_2:-69256061 None:intergenic
GTATGAAGGGTCTAGCATCA+TGG 0.543228 8_2:+69255261 Msa1265150:three_prime_UTR
ATCCGCCACAGCTCTCTCCA+TGG 0.543560 8_2:+69254811 Msa1265150:CDS
AACAGCTATAAGTAGATCCT+CGG 0.546715 8_2:-69255909 None:intergenic
TATGAAGGGTCTAGCATCAT+GGG 0.551384 8_2:+69255262 Msa1265150:three_prime_UTR
AAGGGAACCTCGAGTTGTGA+GGG 0.552694 8_2:+69255111 Msa1265150:CDS
ATGTCTTCCAGCCTGTATGA+AGG 0.554066 8_2:+69255247 Msa1265150:three_prime_UTR
TTAACTATCCTTCAATAATG+TGG 0.555394 8_2:-69255327 None:intergenic
TCAATTAATTCAAAGTTGAG+AGG 0.557443 8_2:-69253596 None:intergenic
AATTATGGTCCTGGTGTCAA+TGG 0.558417 8_2:+69256244 Msa1265150:three_prime_UTR
GAACCTCGAGTTGTGAGGGT+TGG 0.560787 8_2:+69255115 Msa1265150:CDS
GTTAACAACAGAGTTCTGAA+TGG 0.561492 8_2:-69255218 None:intergenic
TGTGGTGTCTTTGATGGAGA+AGG 0.563558 8_2:+69254550 Msa1265150:CDS
GTGTGTAGGTGGTGTTGTTG+TGG 0.565056 8_2:+69256424 Msa1265150:three_prime_UTR
TGGAGTGGCTAATATATGAC+AGG 0.565325 8_2:-69256174 None:intergenic
TGTCTTCCAGCCTGTATGAA+GGG 0.565467 8_2:+69255248 Msa1265150:three_prime_UTR
AAACAAGTCATCAAATGGAC+TGG 0.565498 8_2:+69254308 Msa1265150:CDS
TTTGTGGCTCTGTGTGTAGG+TGG 0.566315 8_2:+69256413 Msa1265150:three_prime_UTR
GAGGTCAAAACCATGTTCCA+TGG 0.583571 8_2:-69254828 None:intergenic
TGTCTGGTGTAGCGGAGGCA+GGG 0.584181 8_2:+69253469 Msa1265150:intron
AGCCTATGCAAGAAGACCAA+TGG 0.586752 8_2:+69255947 Msa1265150:three_prime_UTR
TTGCAGCGTGGTTAGTGTGT+TGG 0.592356 8_2:+69256347 Msa1265150:three_prime_UTR
TTCCATGGAGAGAGCTGTGG+CGG 0.594000 8_2:-69254813 None:intergenic
GGGAGCATGAATTATGGTCC+TGG 0.596278 8_2:+69256235 Msa1265150:three_prime_UTR
TCTTTCTATAGTTAGCAAAG+TGG 0.597790 8_2:-69253676 None:intergenic
ACAGCTCTCTCCATGGAACA+TGG 0.598176 8_2:+69254818 Msa1265150:CDS
TAAGGGAACCTCGAGTTGTG+AGG 0.598332 8_2:+69255110 Msa1265150:CDS
GGATGCAACTTCTTTCGTCA+AGG 0.598987 8_2:+69255968 Msa1265150:three_prime_UTR
TTTGCATGATTTGGCCTCCG+AGG 0.600110 8_2:+69255892 Msa1265150:three_prime_UTR
CTGGTGTAGCGGAGGCAGGG+AGG 0.603912 8_2:+69253472 Msa1265150:intron
AAAGTGCGCAATTGTTGGGA+GGG 0.605427 8_2:-69255158 None:intergenic
AACTTAGGTCTGGATTTGAA+GGG 0.615955 8_2:-69256370 None:intergenic
AAACCAACCCTCACAACTCG+AGG 0.620062 8_2:-69255118 None:intergenic
CTGGTGTCAATGGTAGAAGT+TGG 0.621412 8_2:+69256254 Msa1265150:three_prime_UTR
TAAAGTGCGCAATTGTTGGG+AGG 0.630263 8_2:-69255159 None:intergenic
TCATCAACATTAGCCTCACA+AGG 0.630986 8_2:+69252989 Msa1265150:five_prime_UTR
AAAGGTCTTAAACTTATCTG+CGG 0.650638 8_2:-69254497 None:intergenic
CAACTTCTACCATTGACACC+AGG 0.652303 8_2:-69256253 None:intergenic
CTTCATAGACAACACATGCA+AGG 0.656238 8_2:-69254351 None:intergenic
TCTACTGCACCATCATTGCA+AGG 0.665556 8_2:+69253532 Msa1265150:five_prime_UTR
ATGTTCCATGGAGAGAGCTG+TGG 0.669038 8_2:-69254816 None:intergenic
AATTAAATTATGGCAAAGAG+CGG 0.674865 8_2:-69256110 None:intergenic
CATGGAAGTGTAGACACCAA+AGG 0.678495 8_2:-69254515 None:intergenic
GTGAACATAAGCGAAATGCA+AGG 0.684278 8_2:+69254030 Msa1265150:exon
CTACCACCACTAAAACAGCA+TGG 0.690939 8_2:-69256461 None:intergenic
GAAGGGTCTAGCATCATGGG+AGG 0.706115 8_2:+69255265 Msa1265150:three_prime_UTR
AGCTATAAGTAGATCCTCGG+AGG 0.708824 8_2:-69255906 None:intergenic
ACACGCCGAAGAAAACTGTG+AGG 0.784742 8_2:-69253509 None:intergenic
TTGTTGCCATGATTGCAGCG+TGG 0.786597 8_2:+69256335 Msa1265150:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTATGTTTTGTTTTT+AGG + chr8_2:69253839-69253858 Msa1265150:intron 10.0%
!!! ATTATTATGTTTTGTTTTTA+GGG + chr8_2:69253840-69253859 Msa1265150:intron 10.0%
!!! TATTATTATGTTTTGTTTTT+AGG + chr8_2:69253839-69253858 Msa1265150:intron 10.0%
!!! ATTATTATGTTTTGTTTTTA+GGG + chr8_2:69253840-69253859 Msa1265150:intron 10.0%
!! AATTATTTATCAAATAAGAA+GGG - chr8_2:69254648-69254667 None:intergenic 10.0%
!! TAATTATTTATCAAATAAGA+AGG - chr8_2:69254649-69254668 None:intergenic 10.0%
!!! ATTTTAAGTTTATTTATTTC+AGG + chr8_2:69255057-69255076 Msa1265150:intron 10.0%
!! TTATATGGATAATTAAATTA+TGG - chr8_2:69256123-69256142 None:intergenic 10.0%
!! GTTAAATAAAAAAAAGTTTA+TGG + chr8_2:69256214-69256233 Msa1265150:three_prime_UTR 10.0%
!! AACATAATAATAAGAATGAT+TGG - chr8_2:69253914-69253933 None:intergenic 15.0%
!!! TATTATTGTTATGGATTATT+AGG - chr8_2:69255607-69255626 None:intergenic 15.0%
!! AAAAATAAACACAAGAAAAC+AGG - chr8_2:69252778-69252797 None:intergenic 20.0%
!! AAACAAATCAAACACAAATA+AGG - chr8_2:69253065-69253084 None:intergenic 20.0%
!!! GGATCATTTAAAACATATTA+GGG - chr8_2:69253159-69253178 None:intergenic 20.0%
!!! TGGATCATTTAAAACATATT+AGG - chr8_2:69253160-69253179 None:intergenic 20.0%
!! ATTTGAAGAATGACAAATAT+GGG - chr8_2:69253186-69253205 None:intergenic 20.0%
!! TATTTGAAGAATGACAAATA+TGG - chr8_2:69253187-69253206 None:intergenic 20.0%
!! ATATAATTTACCATGTTGTT+TGG + chr8_2:69253273-69253292 Msa1265150:intron 20.0%
!!! TAGCTTATGAAATATGATTT+TGG + chr8_2:69253347-69253366 Msa1265150:intron 20.0%
!!! ACAAAATGATTTTGTGATAA+TGG + chr8_2:69253731-69253750 Msa1265150:intron 20.0%
!! TTTATCTTGTGATATCATTA+GGG - chr8_2:69254879-69254898 None:intergenic 20.0%
!!! TTTTATCTTGTGATATCATT+AGG - chr8_2:69254880-69254899 None:intergenic 20.0%
!! TAATGATATCACAAGATAAA+AGG + chr8_2:69254879-69254898 Msa1265150:intron 20.0%
!!! GTTATTTAAGCTATAACTTT+TGG + chr8_2:69255023-69255042 Msa1265150:intron 20.0%
!! TGTTTACTTAAAGAAAAAAC+GGG - chr8_2:69255374-69255393 None:intergenic 20.0%
!! GTGTTTACTTAAAGAAAAAA+CGG - chr8_2:69255375-69255394 None:intergenic 20.0%
!!! TTTTTCTTTAAGTAAACACA+TGG + chr8_2:69255376-69255395 Msa1265150:three_prime_UTR 20.0%
!! AACCAAAGAAAAAAAAAAGA+AGG + chr8_2:69255560-69255579 Msa1265150:three_prime_UTR 20.0%
!!! TACCTTCTTTTTTTTTTCTT+TGG - chr8_2:69255565-69255584 None:intergenic 20.0%
!! TCCATAACAATAATAGTAAA+AGG + chr8_2:69255612-69255631 Msa1265150:three_prime_UTR 20.0%
!!! ACCTTTTACTATTATTGTTA+TGG - chr8_2:69255616-69255635 None:intergenic 20.0%
!!! TGTGTTTTATTTCTGTTTTA+TGG + chr8_2:69256507-69256526 Msa1265150:three_prime_UTR 20.0%
! ACATATTAGGGTGATAAATT+TGG - chr8_2:69253147-69253166 None:intergenic 25.0%
!!! TTATATTTTGAACTCTAACC+TGG - chr8_2:69253259-69253278 None:intergenic 25.0%
!! AGTTATTGTGTTTTGTCATT+TGG + chr8_2:69253370-69253389 Msa1265150:intron 25.0%
!! TTTCTTCAAGATTTTGTGTT+TGG + chr8_2:69253411-69253430 Msa1265150:intron 25.0%
! TATGAGATTGAATTTATGTC+TGG + chr8_2:69253453-69253472 Msa1265150:intron 25.0%
! TCAATTAATTCAAAGTTGAG+AGG - chr8_2:69253599-69253618 None:intergenic 25.0%
! TTGCTAACTATAGAAAGAAA+TGG + chr8_2:69253681-69253700 Msa1265150:five_prime_UTR 25.0%
!!! TAAGTGTCATTTAGTGATAT+TGG + chr8_2:69253767-69253786 Msa1265150:intron 25.0%
!!! AAGTGTCATTTAGTGATATT+GGG + chr8_2:69253768-69253787 Msa1265150:intron 25.0%
! TGTAAAGCTTTCAAAATACA+AGG + chr8_2:69254095-69254114 Msa1265150:intron 25.0%
! TAATCCTCTCTTTCATTTAT+AGG + chr8_2:69254198-69254217 Msa1265150:intron 25.0%
! AATCCTCTCTTTCATTTATA+GGG + chr8_2:69254199-69254218 Msa1265150:intron 25.0%
!!! AGTTTTGGAAACAAATGAAA+AGG + chr8_2:69254274-69254293 Msa1265150:CDS 25.0%
!! TATTTTCTAATTTTCGGTGA+GGG - chr8_2:69254403-69254422 None:intergenic 25.0%
!! TTATTTTCTAATTTTCGGTG+AGG - chr8_2:69254404-69254423 None:intergenic 25.0%
!!! TGATTTTGATTTTTCTCAGT+TGG + chr8_2:69254451-69254470 Msa1265150:intron 25.0%
! TTTCTTCTAATTTCACTTCT+TGG + chr8_2:69254589-69254608 Msa1265150:intron 25.0%
! TTCTTCTAATTTCACTTCTT+GGG + chr8_2:69254590-69254609 Msa1265150:intron 25.0%
! CTACAATATAATCATAATCG+CGG - chr8_2:69254738-69254757 None:intergenic 25.0%
!!! ATGCTGATAAAGAAGTATTA+AGG + chr8_2:69255092-69255111 Msa1265150:CDS 25.0%
!!! TGCTGATAAAGAAGTATTAA+GGG + chr8_2:69255093-69255112 Msa1265150:CDS 25.0%
! TTAACTATCCTTCAATAATG+TGG - chr8_2:69255330-69255349 None:intergenic 25.0%
! ATTATTGAAGGATAGTTAAC+TGG + chr8_2:69255331-69255350 Msa1265150:three_prime_UTR 25.0%
! TATTAGGACTGCTTAATTTA+AGG - chr8_2:69255591-69255610 None:intergenic 25.0%
! ATGTATCATAAGCATATTGT+TGG + chr8_2:69255695-69255714 Msa1265150:three_prime_UTR 25.0%
!! GATATTCCTTCATTTGATTT+AGG + chr8_2:69255992-69256011 Msa1265150:three_prime_UTR 25.0%
! AATTAAATTATGGCAAAGAG+CGG - chr8_2:69256113-69256132 None:intergenic 25.0%
! AGCTTAAAAAAACTCTCAAT+TGG - chr8_2:69256149-69256168 None:intergenic 25.0%
!! TTTCTGTTTTATGGTTGATT+TGG + chr8_2:69256516-69256535 Msa1265150:three_prime_UTR 25.0%
ATGAGTGAATACATGTCAAA+TGG - chr8_2:69252892-69252911 None:intergenic 30.0%
TAAAAACATCAAACCTTGTG+AGG - chr8_2:69253005-69253024 None:intergenic 30.0%
! AAGATTTTGTGTTTGGTCAT+TGG + chr8_2:69253418-69253437 Msa1265150:intron 30.0%
AAGGAATGCAATAAGACATT+GGG + chr8_2:69253551-69253570 Msa1265150:five_prime_UTR 30.0%
TCTAACTTTCATCACATTCA+TGG - chr8_2:69253642-69253661 None:intergenic 30.0%
TCTTTCTATAGTTAGCAAAG+TGG - chr8_2:69253679-69253698 None:intergenic 30.0%
AATGGTACGATAGATAAGTA+AGG + chr8_2:69253699-69253718 Msa1265150:intron 30.0%
ATGGTACGATAGATAAGTAA+GGG + chr8_2:69253700-69253719 Msa1265150:intron 30.0%
!! AGTGTCATTTAGTGATATTG+GGG + chr8_2:69253769-69253788 Msa1265150:intron 30.0%
AAGAATGAAAGTGATGATCA+TGG - chr8_2:69253820-69253839 None:intergenic 30.0%
TAGTGTTGTGATTCCTATTA+AGG + chr8_2:69253880-69253899 Msa1265150:intron 30.0%
AGTGTTGTGATTCCTATTAA+GGG + chr8_2:69253881-69253900 Msa1265150:intron 30.0%
! TAGTGTTGAGATTCCTATTA+AGG + chr8_2:69253963-69253982 Msa1265150:five_prime_UTR 30.0%
! AGTGTTGAGATTCCTATTAA+GGG + chr8_2:69253964-69253983 Msa1265150:five_prime_UTR 30.0%
!! CCACTTTAACTTTTGGATTT+TGG - chr8_2:69253997-69254016 None:intergenic 30.0%
CCAAAATCCAAAAGTTAAAG+TGG + chr8_2:69253994-69254013 Msa1265150:five_prime_UTR 30.0%
!!! AACAATGCCACTTTAACTTT+TGG - chr8_2:69254004-69254023 None:intergenic 30.0%
AAAAGTTAAAGTGGCATTGT+TGG + chr8_2:69254003-69254022 Msa1265150:five_prime_UTR 30.0%
! CTTTGTTTCTTTGATGTGTT+TGG + chr8_2:69254135-69254154 Msa1265150:intron 30.0%
ATCCTCTCTTTCATTTATAG+GGG + chr8_2:69254200-69254219 Msa1265150:intron 30.0%
GGAAACAAATGAAAAGGAAT+TGG + chr8_2:69254280-69254299 Msa1265150:CDS 30.0%
TCATGAAACAAGTCATCAAA+TGG + chr8_2:69254303-69254322 Msa1265150:CDS 30.0%
AAAGGTCTTAAACTTATCTG+CGG - chr8_2:69254500-69254519 None:intergenic 30.0%
TAATGAAACAATTGCTCTAC+CGG + chr8_2:69254787-69254806 Msa1265150:CDS 30.0%
!! TTAACAACAGAGTTCTGAAT+GGG - chr8_2:69255220-69255239 None:intergenic 30.0%
CTTCATAACCACATTATTGA+AGG + chr8_2:69255319-69255338 Msa1265150:three_prime_UTR 30.0%
!! ATCTTGTGTCTACTTTTCTT+TGG - chr8_2:69255519-69255538 None:intergenic 30.0%
! TACAGAATTTAGAGTCGTAA+AGG - chr8_2:69255816-69255835 None:intergenic 30.0%
! TTTTGTCTCTTTGCATGATT+TGG + chr8_2:69255883-69255902 Msa1265150:three_prime_UTR 30.0%
TGACTACCTAAATCAAATGA+AGG - chr8_2:69256001-69256020 None:intergenic 30.0%
ACTCTCAATTGGAAGTTATA+TGG - chr8_2:69256138-69256157 None:intergenic 30.0%
AACGAAAAAAATGTCTTCTG+TGG - chr8_2:69256197-69256216 None:intergenic 30.0%
!!! CGATCTTGGTTATTTTTACA+AGG + chr8_2:69256300-69256319 Msa1265150:three_prime_UTR 30.0%
TGAAGAATGACAAATATGGG+AGG - chr8_2:69253183-69253202 None:intergenic 35.0%
TTCTTCGATACCAAACAACA+TGG - chr8_2:69253286-69253305 None:intergenic 35.0%
TGAATTTATGTCTGGTGTAG+CGG + chr8_2:69253461-69253480 Msa1265150:intron 35.0%
TATTGCATTCCTTGCAATGA+TGG - chr8_2:69253544-69253563 None:intergenic 35.0%
CAAGGAATGCAATAAGACAT+TGG + chr8_2:69253550-69253569 Msa1265150:five_prime_UTR 35.0%
CAATAAGACATTGGGATGAT+GGG + chr8_2:69253559-69253578 Msa1265150:five_prime_UTR 35.0%
TCAAAGTTGAGAGGCTAAAT+TGG - chr8_2:69253590-69253609 None:intergenic 35.0%
GCTAAGATCAGCAAAATTTC+AGG + chr8_2:69254054-69254073 Msa1265150:CDS 35.0%
ACAAAGCAACAAAATCACAG+AGG - chr8_2:69254121-69254140 None:intergenic 35.0%
TACCCCTATAAATGAAAGAG+AGG - chr8_2:69254205-69254224 None:intergenic 35.0%
ATTGTTTGTAGCAATTCTGC+AGG + chr8_2:69254245-69254264 Msa1265150:intron 35.0%
! TTCTGCAGGTATGAAAGTTT+TGG + chr8_2:69254259-69254278 Msa1265150:intron 35.0%
AAACAAGTCATCAAATGGAC+TGG + chr8_2:69254308-69254327 Msa1265150:CDS 35.0%
GCAAGGTCAATACTATGTTT+GGG - chr8_2:69254337-69254356 None:intergenic 35.0%
TGCAAGGTCAATACTATGTT+TGG - chr8_2:69254338-69254357 None:intergenic 35.0%
! GCAGATAAGTTTAAGACCTT+TGG + chr8_2:69254499-69254518 Msa1265150:CDS 35.0%
! TCCATGTTTTGCAAAGATTG+TGG + chr8_2:69254532-69254551 Msa1265150:CDS 35.0%
ACCACAATCTTTGCAAAACA+TGG - chr8_2:69254536-69254555 None:intergenic 35.0%
AAAGATTGTGGTGTCTTTGA+TGG + chr8_2:69254544-69254563 Msa1265150:CDS 35.0%
TGAAGTGTATTCATACCTTG+AGG - chr8_2:69254850-69254869 None:intergenic 35.0%
! TCACAAGATAAAAGGTATGC+TGG + chr8_2:69254887-69254906 Msa1265150:intron 35.0%
ACAGATAAGTTTCAATGCTG+AGG - chr8_2:69255005-69255024 None:intergenic 35.0%
GCACTTTATCGAGCAAAAAA+AGG + chr8_2:69255174-69255193 Msa1265150:CDS 35.0%
!! GTTAACAACAGAGTTCTGAA+TGG - chr8_2:69255221-69255240 None:intergenic 35.0%
! TAACTGGATTTTGCCAAGTT+AGG + chr8_2:69255347-69255366 Msa1265150:three_prime_UTR 35.0%
! AACTGGATTTTGCCAAGTTA+GGG + chr8_2:69255348-69255367 Msa1265150:three_prime_UTR 35.0%
ATAATCATGCTGACAGTTTC+TGG + chr8_2:69255661-69255680 Msa1265150:three_prime_UTR 35.0%
!! AGAGTGATGCTTTCTAAGAT+GGG + chr8_2:69255729-69255748 Msa1265150:three_prime_UTR 35.0%
AACAGCTATAAGTAGATCCT+CGG - chr8_2:69255912-69255931 None:intergenic 35.0%
TATTCTGCAATGGTTGAATC+AGG - chr8_2:69256054-69256073 None:intergenic 35.0%
! AGCACATTGTTATTCTGCAA+TGG - chr8_2:69256064-69256083 None:intergenic 35.0%
AAAAAATGTCTTCTGTGGAG+TGG - chr8_2:69256192-69256211 None:intergenic 35.0%
! GTTTATGGGAGCATGAATTA+TGG + chr8_2:69256229-69256248 Msa1265150:three_prime_UTR 35.0%
AACTTAGGTCTGGATTTGAA+GGG - chr8_2:69256373-69256392 None:intergenic 35.0%
AAACTTAGGTCTGGATTTGA+AGG - chr8_2:69256374-69256393 None:intergenic 35.0%
!! ATGGTTGATTTGGTGTTGTT+TGG + chr8_2:69256526-69256545 Msa1265150:three_prime_UTR 35.0%
! TCATCAACATTAGCCTCACA+AGG + chr8_2:69252989-69253008 Msa1265150:five_prime_UTR 40.0%
AGAATGACAAATATGGGAGG+TGG - chr8_2:69253180-69253199 None:intergenic 40.0%
GCTCTGCAGATTTATTTACC+AGG + chr8_2:69253238-69253257 Msa1265150:intron 40.0%
! GCAATCCTCACAGTTTTCTT+CGG + chr8_2:69253504-69253523 Msa1265150:five_prime_UTR 40.0%
GCAATAAGACATTGGGATGA+TGG + chr8_2:69253558-69253577 Msa1265150:five_prime_UTR 40.0%
GTCATTTAGTGATATTGGGG+TGG + chr8_2:69253772-69253791 Msa1265150:intron 40.0%
ATTGGAGCAACACCCTTAAT+AGG - chr8_2:69253896-69253915 None:intergenic 40.0%
! TTTGGAGCAACACCCTTAAT+AGG - chr8_2:69253979-69253998 None:intergenic 40.0%
GTGAACATAAGCGAAATGCA+AGG + chr8_2:69254030-69254049 Msa1265150:exon 40.0%
CTTCATAGACAACACATGCA+AGG - chr8_2:69254354-69254373 None:intergenic 40.0%
GTCTATGAAGATCAAAGTGC+AGG + chr8_2:69254364-69254383 Msa1265150:CDS 40.0%
! TTCTAATTTTCGGTGAGGGT+AGG - chr8_2:69254399-69254418 None:intergenic 40.0%
TGTCTTTGATGGAGAAGGTA+AGG + chr8_2:69254555-69254574 Msa1265150:intron 40.0%
GTCTTTGATGGAGAAGGTAA+GGG + chr8_2:69254556-69254575 Msa1265150:intron 40.0%
GTTCAATTTACAGCCTCATG+TGG + chr8_2:69254670-69254689 Msa1265150:intron 40.0%
! TTACAGCCTCATGTGGATTT+TGG + chr8_2:69254677-69254696 Msa1265150:intron 40.0%
! TACAGCCTCATGTGGATTTT+GGG + chr8_2:69254678-69254697 Msa1265150:intron 40.0%
GCATACACTCCATCAGTTTA+AGG - chr8_2:69254947-69254966 None:intergenic 40.0%
CGATAAAGTGCGCAATTGTT+GGG - chr8_2:69255165-69255184 None:intergenic 40.0%
TCGATAAAGTGCGCAATTGT+TGG - chr8_2:69255166-69255185 None:intergenic 40.0%
TATGAAGGGTCTAGCATCAT+GGG + chr8_2:69255262-69255281 Msa1265150:three_prime_UTR 40.0%
! GAGAGTGATGCTTTCTAAGA+TGG + chr8_2:69255728-69255747 Msa1265150:three_prime_UTR 40.0%
!! ATCCATTGGTCTTCTTGCAT+AGG - chr8_2:69255952-69255971 None:intergenic 40.0%
ACGAAAGAAGTTGCATCCAT+TGG - chr8_2:69255966-69255985 None:intergenic 40.0%
TGGAGTGGCTAATATATGAC+AGG - chr8_2:69256177-69256196 None:intergenic 40.0%
! AATTATGGTCCTGGTGTCAA+TGG + chr8_2:69256244-69256263 Msa1265150:three_prime_UTR 40.0%
CCACAAAAAGTTGCTACAGA+CGG - chr8_2:69256400-69256419 None:intergenic 40.0%
CTGCATGCTAGATATGGACT+GGG - chr8_2:69253097-69253116 None:intergenic 45.0%
CAGTCCATATCTAGCATGCA+GGG + chr8_2:69253096-69253115 Msa1265150:intron 45.0%
CAATCCCTGCATGCTAGATA+TGG - chr8_2:69253103-69253122 None:intergenic 45.0%
ATTTATGTCTGGTGTAGCGG+AGG + chr8_2:69253464-69253483 Msa1265150:intron 45.0%
TCTACTGCACCATCATTGCA+AGG + chr8_2:69253532-69253551 Msa1265150:five_prime_UTR 45.0%
!! AGACATTGGGATGATGGGTT+TGG + chr8_2:69253564-69253583 Msa1265150:five_prime_UTR 45.0%
! CATGGAAGTGTAGACACCAA+AGG - chr8_2:69254518-69254537 None:intergenic 45.0%
TGTGGTGTCTTTGATGGAGA+AGG + chr8_2:69254550-69254569 Msa1265150:CDS 45.0%
!!! GATTTTGGGTTGGCGATATC+TGG + chr8_2:69254692-69254711 Msa1265150:intron 45.0%
GAGGTCAAAACCATGTTCCA+TGG - chr8_2:69254831-69254850 None:intergenic 45.0%
!!! GGAACATGGTTTTGACCTCA+AGG + chr8_2:69254832-69254851 Msa1265150:CDS 45.0%
!! GCTGTGTTGCTTCCTTAAAC+TGG + chr8_2:69254919-69254938 Msa1265150:intron 45.0%
! CAGTTTAAGGCGCCAGTTTA+AGG - chr8_2:69254934-69254953 None:intergenic 45.0%
AAACTGGCGCCTTAAACTGA+TGG + chr8_2:69254935-69254954 Msa1265150:intron 45.0%
TGCTCGATTTCTCGACAACA+CGG + chr8_2:69254964-69254983 Msa1265150:intron 45.0%
AAAGTGCGCAATTGTTGGGA+GGG - chr8_2:69255161-69255180 None:intergenic 45.0%
TAAAGTGCGCAATTGTTGGG+AGG - chr8_2:69255162-69255181 None:intergenic 45.0%
ATGTCTTCCAGCCTGTATGA+AGG + chr8_2:69255247-69255266 Msa1265150:three_prime_UTR 45.0%
TGTCTTCCAGCCTGTATGAA+GGG + chr8_2:69255248-69255267 Msa1265150:three_prime_UTR 45.0%
TGATGCTAGACCCTTCATAC+AGG - chr8_2:69255261-69255280 None:intergenic 45.0%
GTATGAAGGGTCTAGCATCA+TGG + chr8_2:69255261-69255280 Msa1265150:three_prime_UTR 45.0%
AGAAAAAACGGGCCCTAACT+TGG - chr8_2:69255363-69255382 None:intergenic 45.0%
AGCTATAAGTAGATCCTCGG+AGG - chr8_2:69255909-69255928 None:intergenic 45.0%
AGCCTATGCAAGAAGACCAA+TGG + chr8_2:69255947-69255966 Msa1265150:three_prime_UTR 45.0%
GGATGCAACTTCTTTCGTCA+AGG + chr8_2:69255968-69255987 Msa1265150:three_prime_UTR 45.0%
CAACTTCTACCATTGACACC+AGG - chr8_2:69256256-69256275 None:intergenic 45.0%
! CTGGTGTCAATGGTAGAAGT+TGG + chr8_2:69256254-69256273 Msa1265150:three_prime_UTR 45.0%
CGATCTATATGCTGCGATCT+TGG + chr8_2:69256286-69256305 Msa1265150:three_prime_UTR 45.0%
CAAAAAGTTGCTACAGACGG+AGG - chr8_2:69256397-69256416 None:intergenic 45.0%
! CCGTCTGTAGCAACTTTTTG+TGG + chr8_2:69256397-69256416 Msa1265150:three_prime_UTR 45.0%
!! CTTTTTGTGGCTCTGTGTGT+AGG + chr8_2:69256410-69256429 Msa1265150:three_prime_UTR 45.0%
CTACCACCACTAAAACAGCA+TGG - chr8_2:69256464-69256483 None:intergenic 45.0%
!!! AATTATTATTTTCTAATTTT+CGG - chr8_2:69254409-69254428 None:intergenic 5.0%
!! TTAAATAAAAAAAAGTTTAT+GGG + chr8_2:69256215-69256234 Msa1265150:three_prime_UTR 5.0%
CCTGCATGCTAGATATGGAC+TGG - chr8_2:69253098-69253117 None:intergenic 50.0%
CCAGTCCATATCTAGCATGC+AGG + chr8_2:69253095-69253114 Msa1265150:intron 50.0%
ACACGCCGAAGAAAACTGTG+AGG - chr8_2:69253512-69253531 None:intergenic 50.0%
!! GCCTCATGTGGATTTTGGGT+TGG + chr8_2:69254682-69254701 Msa1265150:intron 50.0%
GCCAACCCAAAATCCACATG+AGG - chr8_2:69254686-69254705 None:intergenic 50.0%
!! TTTGGGTTGGCGATATCTGG+AGG + chr8_2:69254695-69254714 Msa1265150:intron 50.0%
!! TTGGGTTGGCGATATCTGGA+GGG + chr8_2:69254696-69254715 Msa1265150:intron 50.0%
ATGTTCCATGGAGAGAGCTG+TGG - chr8_2:69254819-69254838 None:intergenic 50.0%
ACAGCTCTCTCCATGGAACA+TGG + chr8_2:69254818-69254837 Msa1265150:CDS 50.0%
! TAAGGGAACCTCGAGTTGTG+AGG + chr8_2:69255110-69255129 Msa1265150:CDS 50.0%
! AAGGGAACCTCGAGTTGTGA+GGG + chr8_2:69255111-69255130 Msa1265150:CDS 50.0%
AAACCAACCCTCACAACTCG+AGG - chr8_2:69255121-69255140 None:intergenic 50.0%
! TTTGCATGATTTGGCCTCCG+AGG + chr8_2:69255892-69255911 Msa1265150:three_prime_UTR 50.0%
! GGGAGCATGAATTATGGTCC+TGG + chr8_2:69256235-69256254 Msa1265150:three_prime_UTR 50.0%
TTGTTGCCATGATTGCAGCG+TGG + chr8_2:69256335-69256354 Msa1265150:three_prime_UTR 50.0%
CACTAACCACGCTGCAATCA+TGG - chr8_2:69256344-69256363 None:intergenic 50.0%
TTGCAGCGTGGTTAGTGTGT+TGG + chr8_2:69256347-69256366 Msa1265150:three_prime_UTR 50.0%
AGACGGAGGAAACTTAGGTC+TGG - chr8_2:69256383-69256402 None:intergenic 50.0%
GCTACAGACGGAGGAAACTT+AGG - chr8_2:69256388-69256407 None:intergenic 50.0%
! TTTGTGGCTCTGTGTGTAGG+TGG + chr8_2:69256413-69256432 Msa1265150:three_prime_UTR 50.0%
!! GTGTGTAGGTGGTGTTGTTG+TGG + chr8_2:69256424-69256443 Msa1265150:three_prime_UTR 50.0%
!! TAGGTGGTGTTGTTGTGGTC+AGG + chr8_2:69256429-69256448 Msa1265150:three_prime_UTR 50.0%
!!! AGCAGGCCATGCTGTTTTAG+TGG + chr8_2:69256455-69256474 Msa1265150:three_prime_UTR 50.0%
!!! AGGCCATGCTGTTTTAGTGG+TGG + chr8_2:69256458-69256477 Msa1265150:three_prime_UTR 50.0%
!! TGGGTTGGCGATATCTGGAG+GGG + chr8_2:69254697-69254716 Msa1265150:intron 55.0%
TTCCATGGAGAGAGCTGTGG+CGG - chr8_2:69254816-69254835 None:intergenic 55.0%
! GAACCTCGAGTTGTGAGGGT+TGG + chr8_2:69255115-69255134 Msa1265150:CDS 55.0%
GCTAGACCCTTCATACAGGC+TGG - chr8_2:69255257-69255276 None:intergenic 55.0%
GAAGGGTCTAGCATCATGGG+AGG + chr8_2:69255265-69255284 Msa1265150:three_prime_UTR 55.0%
TTGTTGTGGTCAGGTGCAGC+AGG + chr8_2:69256438-69256457 Msa1265150:three_prime_UTR 55.0%
! ATGTCTGGTGTAGCGGAGGC+AGG + chr8_2:69253468-69253487 Msa1265150:intron 60.0%
! TGTCTGGTGTAGCGGAGGCA+GGG + chr8_2:69253469-69253488 Msa1265150:intron 60.0%
!! GAGAGAGCTGTGGCGGATTC+CGG - chr8_2:69254809-69254828 None:intergenic 60.0%
ATCCGCCACAGCTCTCTCCA+TGG + chr8_2:69254811-69254830 Msa1265150:CDS 60.0%
! TGTAGCGGAGGCAGGGAGGT+GGG + chr8_2:69253476-69253495 Msa1265150:intron 65.0%
! CTGGTGTAGCGGAGGCAGGG+AGG + chr8_2:69253472-69253491 Msa1265150:intron 70.0%
! GTGTAGCGGAGGCAGGGAGG+TGG + chr8_2:69253475-69253494 Msa1265150:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 69252758 69256549 69252758 ID=Msa1265150;Name=Msa1265150
chr8_2 mRNA 69252758 69256549 69252758 ID=Msa1265150-mRNA-1;Parent=Msa1265150;Name=Msa1265150-mRNA-1;_AED=0.02;_eAED=0.02;_QI=576|0.85|0.75|1|0.42|0.5|8|1303|173
chr8_2 exon 69252758 69253010 69252758 ID=Msa1265150-mRNA-1:exon:22843;Parent=Msa1265150-mRNA-1
chr8_2 exon 69253481 69253702 69253481 ID=Msa1265150-mRNA-1:exon:22844;Parent=Msa1265150-mRNA-1
chr8_2 exon 69253944 69254075 69253944 ID=Msa1265150-mRNA-1:exon:22845;Parent=Msa1265150-mRNA-1
chr8_2 exon 69254267 69254385 69254267 ID=Msa1265150-mRNA-1:exon:22846;Parent=Msa1265150-mRNA-1
chr8_2 exon 69254470 69254571 69254470 ID=Msa1265150-mRNA-1:exon:22847;Parent=Msa1265150-mRNA-1
chr8_2 exon 69254683 69254688 69254683 ID=Msa1265150-mRNA-1:exon:22848;Parent=Msa1265150-mRNA-1
chr8_2 exon 69254758 69254853 69254758 ID=Msa1265150-mRNA-1:exon:22849;Parent=Msa1265150-mRNA-1
chr8_2 exon 69255079 69256549 69255079 ID=Msa1265150-mRNA-1:exon:22850;Parent=Msa1265150-mRNA-1
chr8_2 five_prime_UTR 69252758 69253010 69252758 ID=Msa1265150-mRNA-1:five_prime_utr;Parent=Msa1265150-mRNA-1
chr8_2 five_prime_UTR 69253481 69253702 69253481 ID=Msa1265150-mRNA-1:five_prime_utr;Parent=Msa1265150-mRNA-1
chr8_2 five_prime_UTR 69253944 69254044 69253944 ID=Msa1265150-mRNA-1:five_prime_utr;Parent=Msa1265150-mRNA-1
chr8_2 CDS 69254045 69254075 69254045 ID=Msa1265150-mRNA-1:cds;Parent=Msa1265150-mRNA-1
chr8_2 CDS 69254267 69254385 69254267 ID=Msa1265150-mRNA-1:cds;Parent=Msa1265150-mRNA-1
chr8_2 CDS 69254470 69254571 69254470 ID=Msa1265150-mRNA-1:cds;Parent=Msa1265150-mRNA-1
chr8_2 CDS 69254683 69254688 69254683 ID=Msa1265150-mRNA-1:cds;Parent=Msa1265150-mRNA-1
chr8_2 CDS 69254758 69254853 69254758 ID=Msa1265150-mRNA-1:cds;Parent=Msa1265150-mRNA-1
chr8_2 CDS 69255079 69255246 69255079 ID=Msa1265150-mRNA-1:cds;Parent=Msa1265150-mRNA-1
chr8_2 three_prime_UTR 69255247 69256549 69255247 ID=Msa1265150-mRNA-1:three_prime_utr;Parent=Msa1265150-mRNA-1
Gene Sequence

>Msa1265150

ATGCAAGGTGCTAAGATCAGCAAAATTTCAGGTATGAAAGTTTTGGAAACAAATGAAAAGGAATTGGTCATGAAACAAGTCATCAAATGGACTGGCAACCCAAACATAGTATTGACCTTGCATGTGTTGTCTATGAAGATCAAAGTGCAGTTGGTAGACTTACAAGTTTTTGCGACGCCGCAGATAAGTTTAAGACCTTTGGTGTCTACACTTCCATGTTTTGCAAAGATTGTGGTGTCTTTGATGGAGAAGCCTCATAAATGCAAATCAATAATAAAAATGTTGCTTAATGAAACAATTGCTCTACCGGAATCCGCCACAGCTCTCTCCATGGAACATGGTTTTGACCTCAAGGATTTCTGCTTTCATGCTGATAAAGAAGTATTAAGGGAACCTCGAGTTGTGAGGGTTGGTTTGATTCAGAACTCTATTGCCCTCCCAACAATTGCGCACTTTATCGAGCAAAAAAAGGCTATCTTTGAGAAAGTAAAGCCCATTCAGAACTCTGTTGTTAACTCATGA
Protein sequence

>Msa1265150

MQGAKISKISGMKVLETNEKELVMKQVIKWTGNPNIVLTLHVLSMKIKVQLVDLQVFATPQISLRPLVSTLPCFAKIVVSLMEKPHKCKSIIKMLLNETIALPESATALSMEHGFDLKDFCFHADKEVLREPRVVRVGLIQNSIALPTIAHFIEQKKAIFEKVKPIQNSVVNS*