AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1401630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1401630 MtrunA17_Chr4g0056781 95.620 137 6 0 1 137 1 137 1.69e-93 266
Msa1401630 MtrunA17_Chr5g0398631 65.693 137 47 0 1 137 1 137 1.77e-66 197
Msa1401630 MtrunA17_Chr7g0258171 44.203 138 71 2 3 134 4 141 7.43e-32 110
Msa1401630 MtrunA17_Chr1g0174201 44.928 138 70 3 3 134 4 141 3.16e-30 106
Msa1401630 MtrunA17_Chr7g0245821 47.059 85 45 0 1 85 1 85 8.34e-21 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1401630 AT1G12390.1 69.403 134 41 0 1 134 1 134 3.59e-67 199
Msa1401630 AT1G62880.1 66.418 134 45 0 1 134 1 134 2.42e-60 182
Msa1401630 AT1G12340.1 69.912 113 34 0 22 134 14 126 3.76e-57 174
Msa1401630 AT4G12090.1 51.493 134 65 0 1 134 1 134 2.40e-50 157
Msa1401630 AT3G12180.1 40.769 130 77 0 3 132 4 133 1.89e-34 117

Find 63 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTATTCCTCTCCTTATTT+TGG 0.153767 tig0022713:+12586 Msa1401630:CDS
CCCATATTTGACGTCTATTA+TGG 0.195490 tig0022713:+12805 Msa1401630:three_prime_UTR
TCCATAATAGACGTCAAATA+TGG 0.225659 tig0022713:-12806 None:intergenic
TCAAGTCACTTACCAAAATA+AGG 0.225877 tig0022713:-12598 None:intergenic
AATTGTAATCCTTATTTATC+AGG 0.264438 tig0022713:+10436 Msa1401630:CDS
ATAAATAAGGATTACAATTA+AGG 0.272852 tig0022713:-10432 None:intergenic
CCATTGCCGGTTTCTGTTGT+TGG 0.301957 tig0022713:+12855 Msa1401630:three_prime_UTR
TAGGTGTGGGTTGCCATTGC+CGG 0.314683 tig0022713:+12842 Msa1401630:three_prime_UTR
GGATTACAATTAAGGCAATA+AGG 0.316976 tig0022713:-10424 None:intergenic
AGAGTTTGACCTGATAAATA+AGG 0.330522 tig0022713:-10445 None:intergenic
TGCGGTTTCTACCTTGTAAC+AGG 0.332875 tig0022713:+10747 Msa1401630:CDS
TGCATCTCGAATAAACAAAA+TGG 0.334517 tig0022713:+10692 Msa1401630:CDS
AATCAGATTTATTACATTTG+TGG 0.382109 tig0022713:+12960 Msa1401630:three_prime_UTR
ATTCGAGATGCAGAATCATA+TGG 0.395869 tig0022713:-10681 None:intergenic
CACAAGTTAGAGAAGGAGTA+TGG 0.403315 tig0022713:+10367 Msa1401630:exon
ACCAAAATAAGGAGAGGAAT+AGG 0.409964 tig0022713:-12587 None:intergenic
TATTATCCAACAACAGAAAC+CGG 0.410621 tig0022713:-12861 None:intergenic
TACTCTCATTTGCACATGTA+TGG 0.427124 tig0022713:-12771 None:intergenic
GATTACAATTAAGGCAATAA+GGG 0.439401 tig0022713:-10423 None:intergenic
TCCGAATTGTATTGTACCTC+AGG 0.440586 tig0022713:+10208 Msa1401630:five_prime_UTR
TGTAACAGGACACTGGGTTA+TGG 0.459940 tig0022713:+10761 Msa1401630:CDS
AGATATTCAACATGCTCCCT+TGG 0.463924 tig0022713:+12520 Msa1401630:CDS
ATTATTTCAGTTAATGTGCC+TGG 0.465076 tig0022713:+10632 Msa1401630:intron
CCATAATAGACGTCAAATAT+GGG 0.469280 tig0022713:-12805 None:intergenic
GATTTATATAATCAAATTCA+AGG 0.470288 tig0022713:-10659 None:intergenic
CAGGTTGATCTACACATCAT+TGG 0.489610 tig0022713:+12684 Msa1401630:intron
TCCTGAGGTACAATACAATT+CGG 0.492808 tig0022713:-10209 None:intergenic
GCACGGAGAAGAACAGAAGA+AGG 0.495644 tig0022713:-10255 None:intergenic
GATATTCAACATGCTCCCTT+GGG 0.497374 tig0022713:+12521 Msa1401630:CDS
TAAGGCAATAAGGGTGAAGA+AGG 0.500668 tig0022713:-10414 None:intergenic
TGCGCACGATAGCAGCTATG+AGG 0.504858 tig0022713:-10290 None:intergenic
AATCAAATTCAAGGTCTGCC+AGG 0.521481 tig0022713:-10650 None:intergenic
GTACAGTCAAGGAAAGCATC+TGG 0.522881 tig0022713:+12485 Msa1401630:CDS
CTACCTTGTAACAGGACACT+GGG 0.524936 tig0022713:+10755 Msa1401630:CDS
TCTACCTTGTAACAGGACAC+TGG 0.526257 tig0022713:+10754 Msa1401630:CDS
TAACCCAGTGTCCTGTTACA+AGG 0.527314 tig0022713:-10758 None:intergenic
TATGGACTAACTTCTACTCT+AGG 0.538073 tig0022713:+12823 Msa1401630:three_prime_UTR
AGTGGATCGTTAACATTGGT+TGG 0.545965 tig0022713:+10162 Msa1401630:five_prime_UTR
CTATAATTGTAGTAGAGGTA+AGG 0.555129 tig0022713:-10798 None:intergenic
ACAATCTATAATTGTAGTAG+AGG 0.566636 tig0022713:-10803 None:intergenic
TATTGGTTCCTTGTGTGAAG+TGG 0.568608 tig0022713:+10144 None:intergenic
GTATGGCTGATATATTCGCT+TGG 0.579050 tig0022713:+10384 Msa1401630:exon
GCGCACGATAGCAGCTATGA+GGG 0.582620 tig0022713:-10289 None:intergenic
ACTAACTTCTACTCTAGGTG+TGG 0.588868 tig0022713:+12828 Msa1401630:three_prime_UTR
TCCCTTGGGAAAAGAAGCAA+CGG 0.596591 tig0022713:+12535 Msa1401630:CDS
AATCAGATTTATTACCTGTG+TGG 0.610850 tig0022713:+12915 Msa1401630:three_prime_UTR
GAGGATCGAGGAGGCTCCTG+AGG 0.616363 tig0022713:-10224 None:intergenic
CTAACTTCTACTCTAGGTGT+GGG 0.619154 tig0022713:+12829 Msa1401630:three_prime_UTR
TTACAAGGTAGAAACCGCAC+AGG 0.623085 tig0022713:-10743 None:intergenic
GCAGCTACTGTGATATACTC+AGG 0.626709 tig0022713:-10720 None:intergenic
TTAACGATCCACTTCACACA+AGG 0.634466 tig0022713:-10152 None:intergenic
TGAGAAAACACGAGCCACAC+AGG 0.640841 tig0022713:-12929 None:intergenic
GTGAAGTGGATCGTTAACAT+TGG 0.649511 tig0022713:+10158 Msa1401630:five_prime_UTR
TGAGGGAATTGTGCGCAGCA+CGG 0.650516 tig0022713:-10272 None:intergenic
TCACTTACCAAAATAAGGAG+AGG 0.652566 tig0022713:-12593 None:intergenic
AAGGAAACCTACGAGGATCG+AGG 0.662125 tig0022713:-10236 None:intergenic
ACTCTGATCAGGTACAGTCA+AGG 0.665524 tig0022713:+12474 Msa1401630:intron
CCAACAACAGAAACCGGCAA+TGG 0.671750 tig0022713:-12855 None:intergenic
ATCACAGTAGCTGCCCTGTG+CGG 0.685904 tig0022713:+10729 Msa1401630:CDS
AGTTTAACGATATACAGACA+CGG 0.690419 tig0022713:-12890 None:intergenic
TACAAGGTAGAAACCGCACA+GGG 0.693755 tig0022713:-10742 None:intergenic
GAAACCTACGAGGATCGAGG+AGG 0.719010 tig0022713:-10233 None:intergenic
ACAGAAGAAGGAAACCTACG+AGG 0.762755 tig0022713:-10243 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACATTATAATTTTTTTGT+AGG + tig0022713:11517-11536 Msa1401630:intron 10.0%
!! ATAAATAAGGATTACAATTA+AGG - tig0022713:10435-10454 None:intergenic 15.0%
!! AGTTGACTAAAAATAAAAAA+GGG - tig0022713:10495-10514 None:intergenic 15.0%
!! AAGTTGACTAAAAATAAAAA+AGG - tig0022713:10496-10515 None:intergenic 15.0%
!! GATTTATATAATCAAATTCA+AGG - tig0022713:10662-10681 None:intergenic 15.0%
!!! TCATTAACAATAAATTTTCA+TGG - tig0022713:10863-10882 None:intergenic 15.0%
!! TAAAAATAGAGGATTAATTA+AGG + tig0022713:11617-11636 Msa1401630:intron 15.0%
!!! AAATATGACTATTATTTTGA+GGG - tig0022713:12203-12222 None:intergenic 15.0%
!!! AATTGTAATCCTTATTTATC+AGG + tig0022713:10436-10455 Msa1401630:CDS 20.0%
!! TTGACTAAAAATAAAAAAGG+GGG - tig0022713:10493-10512 None:intergenic 20.0%
!! GTTGACTAAAAATAAAAAAG+GGG - tig0022713:10494-10513 None:intergenic 20.0%
!! AAAACAAAGCTTTACATAAT+GGG - tig0022713:10526-10545 None:intergenic 20.0%
!! AAAAACAAAGCTTTACATAA+TGG - tig0022713:10527-10546 None:intergenic 20.0%
!!! TATAATGTTTTTGAGAAACA+TGG + tig0022713:10901-10920 Msa1401630:intron 20.0%
!! TTCTTAGAATCAAAATATCT+AGG - tig0022713:11151-11170 None:intergenic 20.0%
!!! GGTTTGATTTATAAAAATAG+AGG + tig0022713:11606-11625 Msa1401630:intron 20.0%
!!! TCATACTTTATTTATTTGCA+AGG + tig0022713:11853-11872 Msa1401630:intron 20.0%
!!! AATATGACTATTATTTTGAG+GGG - tig0022713:12202-12221 None:intergenic 20.0%
!!! CAAATATGACTATTATTTTG+AGG - tig0022713:12204-12223 None:intergenic 20.0%
!! AATCAGATTTATTACATTTG+TGG + tig0022713:12960-12979 Msa1401630:three_prime_UTR 20.0%
! GATTACAATTAAGGCAATAA+GGG - tig0022713:10426-10445 None:intergenic 25.0%
! AAACAAAGCTTTACATAATG+GGG - tig0022713:10525-10544 None:intergenic 25.0%
!!! TTTTAGCTCAATAATTCAAC+TGG + tig0022713:10544-10563 Msa1401630:intron 25.0%
!! TTTAGCTCAATAATTCAACT+GGG + tig0022713:10545-10564 Msa1401630:intron 25.0%
! ACAATCTATAATTGTAGTAG+AGG - tig0022713:10806-10825 None:intergenic 25.0%
! GAACTACCAAATTGAAAATT+GGG - tig0022713:11307-11326 None:intergenic 25.0%
! AGAACTACCAAATTGAAAAT+TGG - tig0022713:11308-11327 None:intergenic 25.0%
! TCTAGTATAATAAAGCCATA+AGG + tig0022713:11394-11413 Msa1401630:intron 25.0%
! ACAAAAACTAAACCAATTTG+TGG - tig0022713:11420-11439 None:intergenic 25.0%
! TTAAAAAAATCGAACCAATC+AGG + tig0022713:11542-11561 Msa1401630:intron 25.0%
! TATAAATCAAACCATGACAT+GGG - tig0022713:11599-11618 None:intergenic 25.0%
! TTATAAATCAAACCATGACA+TGG - tig0022713:11600-11619 None:intergenic 25.0%
!! ATAAATATCACTTCTTTTGC+TGG - tig0022713:11657-11676 None:intergenic 25.0%
! TACAAAAATTGAGATGATGA+TGG - tig0022713:11751-11770 None:intergenic 25.0%
!! CAATAGCTGTAATTGTATTT+TGG + tig0022713:11829-11848 Msa1401630:intron 25.0%
! AATACAAAGCAGTATCAAAA+GGG - tig0022713:12259-12278 None:intergenic 25.0%
! AAATACAAAGCAGTATCAAA+AGG - tig0022713:12260-12279 None:intergenic 25.0%
!! AACTTTTATGCTGATATAGA+TGG + tig0022713:12294-12313 Msa1401630:intron 25.0%
! TAATAACAAATACTGTTACC+AGG - tig0022713:12455-12474 None:intergenic 25.0%
!!! TATTTGTTATTACTCTGATC+AGG + tig0022713:12463-12482 Msa1401630:intron 25.0%
! ATGCAAGAATTGTAAGTTAA+TGG - tig0022713:12655-12674 None:intergenic 25.0%
! TCAAAAACAACTACTCATAA+GGG - tig0022713:12714-12733 None:intergenic 25.0%
! ATCAAAAACAACTACTCATA+AGG - tig0022713:12715-12734 None:intergenic 25.0%
GAAAAATCACAAGTTAGAGA+AGG + tig0022713:10360-10379 Msa1401630:five_prime_UTR 30.0%
GGATTACAATTAAGGCAATA+AGG - tig0022713:10427-10446 None:intergenic 30.0%
AGAGTTTGACCTGATAAATA+AGG - tig0022713:10448-10467 None:intergenic 30.0%
TAAAAAAGGGGGAAAAGAAT+GGG - tig0022713:10482-10501 None:intergenic 30.0%
ATAAAAAAGGGGGAAAAGAA+TGG - tig0022713:10483-10502 None:intergenic 30.0%
ATTATTTCAGTTAATGTGCC+TGG + tig0022713:10632-10651 Msa1401630:intron 30.0%
TGCATCTCGAATAAACAAAA+TGG + tig0022713:10692-10711 Msa1401630:CDS 30.0%
CTATAATTGTAGTAGAGGTA+AGG - tig0022713:10801-10820 None:intergenic 30.0%
AATTAGTCATTGTTTGCACT+CGG + tig0022713:10928-10947 Msa1401630:intron 30.0%
! GGTAGTTTGAGTTAGAAATT+TGG + tig0022713:10965-10984 Msa1401630:intron 30.0%
TGAGTTAGAAATTTGGATCT+TGG + tig0022713:10972-10991 Msa1401630:intron 30.0%
ATTTGGATCTTGGCATTATT+CGG + tig0022713:10982-11001 Msa1401630:intron 30.0%
! GATTCTAAGAAACTTCTTCA+CGG + tig0022713:11160-11179 Msa1401630:intron 30.0%
! ATTCTAAGAAACTTCTTCAC+GGG + tig0022713:11161-11180 Msa1401630:intron 30.0%
! TTGAAAGAATTTTGTGTCTC+TGG + tig0022713:11221-11240 Msa1401630:intron 30.0%
! GTTCAACCCAATTTTCAATT+TGG + tig0022713:11298-11317 Msa1401630:intron 30.0%
!!! TCTTTTTCCACTTTGTTGTT+TGG + tig0022713:11325-11344 Msa1401630:intron 30.0%
AAGATAACCAAACAACAAAG+TGG - tig0022713:11335-11354 None:intergenic 30.0%
!! ACTTTGTTGTTTGGTTATCT+TGG + tig0022713:11334-11353 Msa1401630:intron 30.0%
!!! TTGGTTTGGTTTGATTTTCT+GGG + tig0022713:11353-11372 Msa1401630:intron 30.0%
GTAGTACTCAGTTTATGTAA+AGG + tig0022713:11491-11510 Msa1401630:intron 30.0%
!! ATTTTTGTTTCATGCACCAA+GGG - tig0022713:11797-11816 None:intergenic 30.0%
TACAATTACAGCTATTGCAT+TGG - tig0022713:11826-11845 None:intergenic 30.0%
!! GTTTGTTGTAATTGCTAAGT+AGG + tig0022713:12028-12047 Msa1401630:intron 30.0%
! TAAATCTCATCCATGTTTTC+TGG + tig0022713:12080-12099 Msa1401630:intron 30.0%
! AAATCTCATCCATGTTTTCT+GGG + tig0022713:12081-12100 Msa1401630:intron 30.0%
!! CAACAACAATAGGTCATTTT+CGG - tig0022713:12141-12160 None:intergenic 30.0%
ACCATGATAGCTTAATAAAC+TGG + tig0022713:12412-12431 Msa1401630:intron 30.0%
TCCAGTTTATTAAGCTATCA+TGG - tig0022713:12416-12435 None:intergenic 30.0%
TCAAGTCACTTACCAAAATA+AGG - tig0022713:12601-12620 None:intergenic 30.0%
TTGGTAAGTGACTTGAAAAA+AGG + tig0022713:12605-12624 Msa1401630:intron 30.0%
ACTTACAATTCTTGCATTTG+CGG + tig0022713:12658-12677 Msa1401630:intron 30.0%
!!! GTTTTTGATTGCTTGTTTTG+TGG + tig0022713:12726-12745 Msa1401630:three_prime_UTR 30.0%
CCATAATAGACGTCAAATAT+GGG - tig0022713:12808-12827 None:intergenic 30.0%
TCCATAATAGACGTCAAATA+TGG - tig0022713:12809-12828 None:intergenic 30.0%
TATTATCCAACAACAGAAAC+CGG - tig0022713:12864-12883 None:intergenic 30.0%
AGTTTAACGATATACAGACA+CGG - tig0022713:12893-12912 None:intergenic 30.0%
AATCAGATTTATTACCTGTG+TGG + tig0022713:12915-12934 Msa1401630:three_prime_UTR 30.0%
TCCTGAGGTACAATACAATT+CGG - tig0022713:10212-10231 None:intergenic 35.0%
ATTCGAGATGCAGAATCATA+TGG - tig0022713:10684-10703 None:intergenic 35.0%
ACTTTATAGCGCTATCAAAC+TGG - tig0022713:11074-11093 None:intergenic 35.0%
! TTCTAAGAAACTTCTTCACG+GGG + tig0022713:11162-11181 Msa1401630:intron 35.0%
TTCATGTGTGATCAGTAGTT+TGG + tig0022713:11190-11209 Msa1401630:intron 35.0%
TCATGTGTGATCAGTAGTTT+GGG + tig0022713:11191-11210 Msa1401630:intron 35.0%
GTTGTTTGGTTATCTTGGTT+TGG + tig0022713:11339-11358 Msa1401630:intron 35.0%
!!! CTTGGTTTGGTTTGATTTTC+TGG + tig0022713:11352-11371 Msa1401630:intron 35.0%
!!! TGGTTTGGTTTGATTTTCTG+GGG + tig0022713:11354-11373 Msa1401630:intron 35.0%
! TAAACCAATTTGTGGCCTTA+TGG - tig0022713:11412-11431 None:intergenic 35.0%
AGAAGATCTAGAAAAGAGTG+AGG - tig0022713:11448-11467 None:intergenic 35.0%
TTTAGTAAGATCCCATGTCA+TGG + tig0022713:11585-11604 Msa1401630:intron 35.0%
!! CATTTTTGTTTCATGCACCA+AGG - tig0022713:11798-11817 None:intergenic 35.0%
! CTTGGTGCATGAAACAAAAA+TGG + tig0022713:11796-11815 Msa1401630:intron 35.0%
TGCACTTCTTCACAATATCA+AGG - tig0022713:11916-11935 None:intergenic 35.0%
!!! ACAGTTTTGGATGTGTTCAT+TGG + tig0022713:12053-12072 Msa1401630:intron 35.0%
! AATCTCATCCATGTTTTCTG+GGG + tig0022713:12082-12101 Msa1401630:intron 35.0%
AAACTGGAATGTGCTTAGTA+TGG + tig0022713:12428-12447 Msa1401630:intron 35.0%
!! TCCTATTCCTCTCCTTATTT+TGG + tig0022713:12586-12605 Msa1401630:CDS 35.0%
ACCAAAATAAGGAGAGGAAT+AGG - tig0022713:12590-12609 None:intergenic 35.0%
TCACTTACCAAAATAAGGAG+AGG - tig0022713:12596-12615 None:intergenic 35.0%
!! TTGTTTTGTGGAATGCTTTC+TGG + tig0022713:12738-12757 Msa1401630:three_prime_UTR 35.0%
TACTCTCATTTGCACATGTA+TGG - tig0022713:12774-12793 None:intergenic 35.0%
CCCATATTTGACGTCTATTA+TGG + tig0022713:12805-12824 Msa1401630:three_prime_UTR 35.0%
! TATGGACTAACTTCTACTCT+AGG + tig0022713:12823-12842 Msa1401630:three_prime_UTR 35.0%
TTAACGATCCACTTCACACA+AGG - tig0022713:10155-10174 None:intergenic 40.0%
GTGAAGTGGATCGTTAACAT+TGG + tig0022713:10158-10177 Msa1401630:five_prime_UTR 40.0%
AGTGGATCGTTAACATTGGT+TGG + tig0022713:10162-10181 Msa1401630:five_prime_UTR 40.0%
TCCGAATTGTATTGTACCTC+AGG + tig0022713:10208-10227 Msa1401630:five_prime_UTR 40.0%
CACAAGTTAGAGAAGGAGTA+TGG + tig0022713:10367-10386 Msa1401630:exon 40.0%
GTATGGCTGATATATTCGCT+TGG + tig0022713:10384-10403 Msa1401630:exon 40.0%
TAAGGCAATAAGGGTGAAGA+AGG - tig0022713:10417-10436 None:intergenic 40.0%
!! GGGGAAAAGAATGGGTTAAA+GGG - tig0022713:10474-10493 None:intergenic 40.0%
AATCAAATTCAAGGTCTGCC+AGG - tig0022713:10653-10672 None:intergenic 40.0%
CACTCGGATTTATGAGTGAA+TGG + tig0022713:10944-10963 Msa1401630:intron 40.0%
TAGCGCTATAAAGTACGTGT+TGG + tig0022713:11080-11099 Msa1401630:intron 40.0%
!! ACGTGTTGGTTGATGTTTCA+TGG + tig0022713:11094-11113 Msa1401630:intron 40.0%
AAAGCCATAAGGCCACAAAT+TGG + tig0022713:11405-11424 Msa1401630:intron 40.0%
! TAATCTGAGTACTGCTGCTA+GGG - tig0022713:11689-11708 None:intergenic 40.0%
! TTAATCTGAGTACTGCTGCT+AGG - tig0022713:11690-11709 None:intergenic 40.0%
! GTCAAGTTTGGTTCAGTTTG+AGG - tig0022713:11729-11748 None:intergenic 40.0%
!! GAGATGATGATGGTCAAGTT+TGG - tig0022713:11741-11760 None:intergenic 40.0%
! GGTATTGTCACTTCTTTCGT+TGG - tig0022713:11776-11795 None:intergenic 40.0%
GAAAGAAGTGACAATACCCT+TGG + tig0022713:11778-11797 Msa1401630:intron 40.0%
! TGCTAAGTAGGCTACAGTTT+TGG + tig0022713:12040-12059 Msa1401630:intron 40.0%
! ATCTCATCCATGTTTTCTGG+GGG + tig0022713:12083-12102 Msa1401630:intron 40.0%
AGAGTTGCTGCAACAACAAT+AGG - tig0022713:12151-12170 None:intergenic 40.0%
AGATATTCAACATGCTCCCT+TGG + tig0022713:12520-12539 Msa1401630:CDS 40.0%
GATATTCAACATGCTCCCTT+GGG + tig0022713:12521-12540 Msa1401630:CDS 40.0%
!! CAGGTTGATCTACACATCAT+TGG + tig0022713:12684-12703 Msa1401630:intron 40.0%
ACTAACTTCTACTCTAGGTG+TGG + tig0022713:12828-12847 Msa1401630:three_prime_UTR 40.0%
CTAACTTCTACTCTAGGTGT+GGG + tig0022713:12829-12848 Msa1401630:three_prime_UTR 40.0%
ACAGAAGAAGGAAACCTACG+AGG - tig0022713:10246-10265 None:intergenic 45.0%
!! GGGGGAAAAGAATGGGTTAA+AGG - tig0022713:10475-10494 None:intergenic 45.0%
GCAGCTACTGTGATATACTC+AGG - tig0022713:10723-10742 None:intergenic 45.0%
TACAAGGTAGAAACCGCACA+GGG - tig0022713:10745-10764 None:intergenic 45.0%
TTACAAGGTAGAAACCGCAC+AGG - tig0022713:10746-10765 None:intergenic 45.0%
TGCGGTTTCTACCTTGTAAC+AGG + tig0022713:10747-10766 Msa1401630:CDS 45.0%
TCTACCTTGTAACAGGACAC+TGG + tig0022713:10754-10773 Msa1401630:CDS 45.0%
CTACCTTGTAACAGGACACT+GGG + tig0022713:10755-10774 Msa1401630:CDS 45.0%
TAACCCAGTGTCCTGTTACA+AGG - tig0022713:10761-10780 None:intergenic 45.0%
TGTAACAGGACACTGGGTTA+TGG + tig0022713:10761-10780 Msa1401630:CDS 45.0%
TGTGATCAGTAGTTTGGGTG+AGG + tig0022713:11196-11215 Msa1401630:intron 45.0%
GTGATCAGTAGTTTGGGTGA+GGG + tig0022713:11197-11216 Msa1401630:intron 45.0%
CCTGCACTCGAGTTATAAAG+CGG - tig0022713:11253-11272 None:intergenic 45.0%
! AATCTGAGTACTGCTGCTAG+GGG - tig0022713:11688-11707 None:intergenic 45.0%
GATGCGACTGCAATTGATCA+TGG + tig0022713:11949-11968 Msa1401630:intron 45.0%
CATATCTCCCCCAGAAAACA+TGG - tig0022713:12093-12112 None:intergenic 45.0%
GAATGTGCTTAGTATGGTCC+TGG + tig0022713:12434-12453 Msa1401630:intron 45.0%
ACTCTGATCAGGTACAGTCA+AGG + tig0022713:12474-12493 Msa1401630:intron 45.0%
!! GTACAGTCAAGGAAAGCATC+TGG + tig0022713:12485-12504 Msa1401630:CDS 45.0%
TCCCTTGGGAAAAGAAGCAA+CGG + tig0022713:12535-12554 Msa1401630:CDS 45.0%
AAGGAAACCTACGAGGATCG+AGG - tig0022713:10239-10258 None:intergenic 50.0%
GCACGGAGAAGAACAGAAGA+AGG - tig0022713:10258-10277 None:intergenic 50.0%
CCGCTTTATAACTCGAGTGC+AGG + tig0022713:11250-11269 Msa1401630:intron 50.0%
ATCGAACCAATCAGGAGCAG+TGG + tig0022713:11550-11569 Msa1401630:intron 50.0%
ACTGAACCACTGCTCCTGAT+TGG - tig0022713:11559-11578 None:intergenic 50.0%
! ATCTGAGTACTGCTGCTAGG+GGG - tig0022713:11687-11706 None:intergenic 50.0%
TTGAGGGGACTGTGCTTCTA+TGG - tig0022713:12187-12206 None:intergenic 50.0%
! GCCGTTGCTTCTTTTCCCAA+GGG - tig0022713:12539-12558 None:intergenic 50.0%
! AGCCGTTGCTTCTTTTCCCA+AGG - tig0022713:12540-12559 None:intergenic 50.0%
ATTCTTGCATTTGCGGCTGC+AGG + tig0022713:12665-12684 Msa1401630:intron 50.0%
CCAACAACAGAAACCGGCAA+TGG - tig0022713:12858-12877 None:intergenic 50.0%
!! CCATTGCCGGTTTCTGTTGT+TGG + tig0022713:12855-12874 Msa1401630:three_prime_UTR 50.0%
TGAGAAAACACGAGCCACAC+AGG - tig0022713:12932-12951 None:intergenic 50.0%
GAAACCTACGAGGATCGAGG+AGG - tig0022713:10236-10255 None:intergenic 55.0%
TGAGGGAATTGTGCGCAGCA+CGG - tig0022713:10275-10294 None:intergenic 55.0%
GCGCACGATAGCAGCTATGA+GGG - tig0022713:10292-10311 None:intergenic 55.0%
TGCGCACGATAGCAGCTATG+AGG - tig0022713:10293-10312 None:intergenic 55.0%
ATCACAGTAGCTGCCCTGTG+CGG + tig0022713:10729-10748 Msa1401630:CDS 55.0%
! TAGGTGTGGGTTGCCATTGC+CGG + tig0022713:12842-12861 Msa1401630:three_prime_UTR 55.0%
GAGGATCGAGGAGGCTCCTG+AGG - tig0022713:10227-10246 None:intergenic 65.0%
GGAGCCTCCTCGATCCTCGT+AGG + tig0022713:10229-10248 Msa1401630:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
tig0022713 gene 10149 13029 10149 ID=Msa1401630;Name=Msa1401630
tig0022713 mRNA 10149 13029 10149 ID=Msa1401630-mRNA-1;Parent=Msa1401630;Name=Msa1401630-mRNA-1;_AED=0.03;_eAED=0.03;_QI=237|1|1|1|1|1|4|306|137
tig0022713 exon 10149 10457 10149 ID=Msa1401630-mRNA-1:exon:18476;Parent=Msa1401630-mRNA-1
tig0022713 exon 10642 10823 10642 ID=Msa1401630-mRNA-1:exon:18477;Parent=Msa1401630-mRNA-1
tig0022713 exon 12485 12607 12485 ID=Msa1401630-mRNA-1:exon:18478;Parent=Msa1401630-mRNA-1
tig0022713 exon 12687 13029 12687 ID=Msa1401630-mRNA-1:exon:18479;Parent=Msa1401630-mRNA-1
tig0022713 five_prime_UTR 10149 10385 10149 ID=Msa1401630-mRNA-1:five_prime_utr;Parent=Msa1401630-mRNA-1
tig0022713 CDS 10386 10457 10386 ID=Msa1401630-mRNA-1:cds;Parent=Msa1401630-mRNA-1
tig0022713 CDS 10642 10823 10642 ID=Msa1401630-mRNA-1:cds;Parent=Msa1401630-mRNA-1
tig0022713 CDS 12485 12607 12485 ID=Msa1401630-mRNA-1:cds;Parent=Msa1401630-mRNA-1
tig0022713 CDS 12687 12723 12687 ID=Msa1401630-mRNA-1:cds;Parent=Msa1401630-mRNA-1
tig0022713 three_prime_UTR 12724 13029 12724 ID=Msa1401630-mRNA-1:three_prime_utr;Parent=Msa1401630-mRNA-1
Gene Sequence

>Msa1401630

ATGGCTGATATATTCGCTTGGCTCTTCTCCTTCTTCACCCTTATTGCCTTAATTGTAATCCTTATTTATCAGTTAATGTGCCTGGCAGACCTTGAATTTGATTATATAAATCCATATGATTCTGCATCTCGAATAAACAAAATGGTTATGCCTGAGTATATCACAGTAGCTGCCCTGTGCGGTTTCTACCTTGTAACAGGACACTGGGTTATGGCACTCTTGTGTGCTCCTTACCTCTACTACAATTATAGATTGTACAGTCAAGGAAAGCATCTGGTTGATGTTACAGAGATATTCAACATGCTCCCTTGGGAAAAGAAGCAACGGCTTTTCAAACTCTTCTATCTGATATTTATCCTATTCCTCTCCTTATTTTGGTTGATCTACACATCATTGGACGACCCTTATGAGTAG
Protein sequence

>Msa1401630

MADIFAWLFSFFTLIALIVILIYQLMCLADLEFDYINPYDSASRINKMVMPEYITVAALCGFYLVTGHWVMALLCAPYLYYNYRLYSQGKHLVDVTEIFNMLPWEKKQRLFKLFYLIFILFLSLFWLIYTSLDDPYE*