AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1258040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1258040 MtrunA17_Chr4g0056781 95.620 137 6 0 1 137 1 137 1.69e-93 266
Msa1258040 MtrunA17_Chr5g0398631 65.693 137 47 0 1 137 1 137 1.77e-66 197
Msa1258040 MtrunA17_Chr7g0258171 44.203 138 71 2 3 134 4 141 7.43e-32 110
Msa1258040 MtrunA17_Chr1g0174201 44.928 138 70 3 3 134 4 141 3.16e-30 106
Msa1258040 MtrunA17_Chr7g0245821 47.059 85 45 0 1 85 1 85 8.34e-21 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1258040 AT1G12390.1 69.403 134 41 0 1 134 1 134 3.59e-67 199
Msa1258040 AT1G62880.1 66.418 134 45 0 1 134 1 134 2.42e-60 182
Msa1258040 AT1G12340.1 69.912 113 34 0 22 134 14 126 3.76e-57 174
Msa1258040 AT4G12090.1 51.493 134 65 0 1 134 1 134 2.40e-50 157
Msa1258040 AT3G12180.1 40.769 130 77 0 3 132 4 133 1.89e-34 117

Find 63 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTATTCCTCTCCTTATTT+TGG 0.153767 8_2:-59134186 Msa1258040:intron
CCCATATTTGACGTCTATTA+TGG 0.195490 8_2:-59133967 Msa1258040:three_prime_UTR
TCCATAATAGACGTCAAATA+TGG 0.225659 8_2:+59133966 None:intergenic
TCAAGTCACTTACCAAAATA+AGG 0.225877 8_2:+59134174 None:intergenic
AATTGTAATCCTTATTTATC+AGG 0.264438 8_2:-59136336 Msa1258040:intron
ATAAATAAGGATTACAATTA+AGG 0.272852 8_2:+59136340 None:intergenic
CCATTGCCGGTTTCTGTTGT+TGG 0.301957 8_2:-59133917 Msa1258040:three_prime_UTR
TAGGTGTGGGTTGCCATTGC+CGG 0.314683 8_2:-59133930 Msa1258040:three_prime_UTR
GGATTACAATTAAGGCAATA+AGG 0.316976 8_2:+59136348 None:intergenic
AGAGTTTGACCTGATAAATA+AGG 0.330522 8_2:+59136327 None:intergenic
TGCGGTTTCTACCTTGTAAC+AGG 0.332875 8_2:-59136025 Msa1258040:CDS
TGCATCTCGAATAAACAAAA+TGG 0.334517 8_2:-59136080 Msa1258040:CDS
AATCAGATTTATTACATTTG+TGG 0.382109 8_2:-59133812 Msa1258040:three_prime_UTR
ATTCGAGATGCAGAATCATA+TGG 0.395869 8_2:+59136091 None:intergenic
CACAAGTTAGAGAAGGAGTA+TGG 0.403315 8_2:-59136405 Msa1258040:exon
ACCAAAATAAGGAGAGGAAT+AGG 0.409964 8_2:+59134185 None:intergenic
TATTATCCAACAACAGAAAC+CGG 0.410621 8_2:+59133911 None:intergenic
TACTCTCATTTGCACATGTA+TGG 0.427124 8_2:+59134001 None:intergenic
GATTACAATTAAGGCAATAA+GGG 0.439401 8_2:+59136349 None:intergenic
TCCGAATTGTATTGTACCTC+AGG 0.440586 8_2:-59136564 Msa1258040:five_prime_UTR
TGTAACAGGACACTGGGTTA+TGG 0.459940 8_2:-59136011 Msa1258040:CDS
AGATATTCAACATGCTCCCT+TGG 0.463924 8_2:-59134252 Msa1258040:CDS
ATTATTTCAGTTAATGTGCC+TGG 0.465076 8_2:-59136140 Msa1258040:intron
CCATAATAGACGTCAAATAT+GGG 0.469280 8_2:+59133967 None:intergenic
GATTTATATAATCAAATTCA+AGG 0.470288 8_2:+59136113 None:intergenic
CAGGTTGATCTACACATCAT+TGG 0.489610 8_2:-59134088 Msa1258040:CDS
TCCTGAGGTACAATACAATT+CGG 0.492808 8_2:+59136563 None:intergenic
GCACGGAGAAGAACAGAAGA+AGG 0.495644 8_2:+59136517 None:intergenic
GATATTCAACATGCTCCCTT+GGG 0.497374 8_2:-59134251 Msa1258040:CDS
TAAGGCAATAAGGGTGAAGA+AGG 0.500668 8_2:+59136358 None:intergenic
TGCGCACGATAGCAGCTATG+AGG 0.504858 8_2:+59136482 None:intergenic
AATCAAATTCAAGGTCTGCC+AGG 0.521481 8_2:+59136122 None:intergenic
GTACAGTCAAGGAAAGCATC+TGG 0.522881 8_2:-59134287 Msa1258040:CDS
CTACCTTGTAACAGGACACT+GGG 0.524936 8_2:-59136017 Msa1258040:CDS
TCTACCTTGTAACAGGACAC+TGG 0.526257 8_2:-59136018 Msa1258040:CDS
TAACCCAGTGTCCTGTTACA+AGG 0.527314 8_2:+59136014 None:intergenic
TATGGACTAACTTCTACTCT+AGG 0.538073 8_2:-59133949 Msa1258040:three_prime_UTR
AGTGGATCGTTAACATTGGT+TGG 0.545965 8_2:-59136610 Msa1258040:five_prime_UTR
CTATAATTGTAGTAGAGGTA+AGG 0.555129 8_2:+59135974 None:intergenic
ACAATCTATAATTGTAGTAG+AGG 0.566636 8_2:+59135969 None:intergenic
TATTGGTTCCTTGTGTGAAG+TGG 0.568608 8_2:-59136628 None:intergenic
GTATGGCTGATATATTCGCT+TGG 0.579050 8_2:-59136388 Msa1258040:CDS
GCGCACGATAGCAGCTATGA+GGG 0.582620 8_2:+59136483 None:intergenic
ACTAACTTCTACTCTAGGTG+TGG 0.588868 8_2:-59133944 Msa1258040:three_prime_UTR
TCCCTTGGGAAAAGAAGCAA+CGG 0.596591 8_2:-59134237 Msa1258040:CDS
AATCAGATTTATTACCTGTG+TGG 0.610850 8_2:-59133857 Msa1258040:three_prime_UTR
GAGGATCGAGGAGGCTCCTG+AGG 0.616363 8_2:+59136548 None:intergenic
CTAACTTCTACTCTAGGTGT+GGG 0.619154 8_2:-59133943 Msa1258040:three_prime_UTR
TTACAAGGTAGAAACCGCAC+AGG 0.623085 8_2:+59136029 None:intergenic
GCAGCTACTGTGATATACTC+AGG 0.626709 8_2:+59136052 None:intergenic
TTAACGATCCACTTCACACA+AGG 0.634466 8_2:+59136620 None:intergenic
TGAGAAAACACGAGCCACAC+AGG 0.640841 8_2:+59133843 None:intergenic
GTGAAGTGGATCGTTAACAT+TGG 0.649511 8_2:-59136614 Msa1258040:five_prime_UTR
TGAGGGAATTGTGCGCAGCA+CGG 0.650516 8_2:+59136500 None:intergenic
TCACTTACCAAAATAAGGAG+AGG 0.652566 8_2:+59134179 None:intergenic
AAGGAAACCTACGAGGATCG+AGG 0.662125 8_2:+59136536 None:intergenic
ACTCTGATCAGGTACAGTCA+AGG 0.665524 8_2:-59134298 Msa1258040:intron
CCAACAACAGAAACCGGCAA+TGG 0.671750 8_2:+59133917 None:intergenic
ATCACAGTAGCTGCCCTGTG+CGG 0.685904 8_2:-59136043 Msa1258040:CDS
AGTTTAACGATATACAGACA+CGG 0.690419 8_2:+59133882 None:intergenic
TACAAGGTAGAAACCGCACA+GGG 0.693755 8_2:+59136030 None:intergenic
GAAACCTACGAGGATCGAGG+AGG 0.719010 8_2:+59136539 None:intergenic
ACAGAAGAAGGAAACCTACG+AGG 0.762755 8_2:+59136529 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACATTATAATTTTTTTGT+AGG - chr8_2:59135133-59135152 Msa1258040:intron 10.0%
!! ATAAATAAGGATTACAATTA+AGG + chr8_2:59134051-59134070 None:intergenic 15.0%
!! AGTTGACTAAAAATAAAAAA+GGG + chr8_2:59134111-59134130 None:intergenic 15.0%
!! AAGTTGACTAAAAATAAAAA+AGG + chr8_2:59134112-59134131 None:intergenic 15.0%
!! GATTTATATAATCAAATTCA+AGG + chr8_2:59134278-59134297 None:intergenic 15.0%
!!! TCATTAACAATAAATTTTCA+TGG + chr8_2:59134479-59134498 None:intergenic 15.0%
!! TAAAAATAGAGGATTAATTA+AGG - chr8_2:59135233-59135252 Msa1258040:intron 15.0%
!!! AAATATGACTATTATTTTGA+GGG + chr8_2:59135819-59135838 None:intergenic 15.0%
!!! AATTGTAATCCTTATTTATC+AGG - chr8_2:59134052-59134071 Msa1258040:exon 20.0%
!! TTGACTAAAAATAAAAAAGG+GGG + chr8_2:59134109-59134128 None:intergenic 20.0%
!! GTTGACTAAAAATAAAAAAG+GGG + chr8_2:59134110-59134129 None:intergenic 20.0%
!! AAAACAAAGCTTTACATAAT+GGG + chr8_2:59134142-59134161 None:intergenic 20.0%
!! AAAAACAAAGCTTTACATAA+TGG + chr8_2:59134143-59134162 None:intergenic 20.0%
!!! TATAATGTTTTTGAGAAACA+TGG - chr8_2:59134517-59134536 Msa1258040:intron 20.0%
!! TTCTTAGAATCAAAATATCT+AGG + chr8_2:59134767-59134786 None:intergenic 20.0%
!!! GGTTTGATTTATAAAAATAG+AGG - chr8_2:59135222-59135241 Msa1258040:intron 20.0%
!!! TCATACTTTATTTATTTGCA+AGG - chr8_2:59135469-59135488 Msa1258040:intron 20.0%
!!! AATATGACTATTATTTTGAG+GGG + chr8_2:59135818-59135837 None:intergenic 20.0%
!!! CAAATATGACTATTATTTTG+AGG + chr8_2:59135820-59135839 None:intergenic 20.0%
!! AATCAGATTTATTACATTTG+TGG - chr8_2:59136576-59136595 Msa1258040:five_prime_UTR 20.0%
! GATTACAATTAAGGCAATAA+GGG + chr8_2:59134042-59134061 None:intergenic 25.0%
! AAACAAAGCTTTACATAATG+GGG + chr8_2:59134141-59134160 None:intergenic 25.0%
!!! TTTTAGCTCAATAATTCAAC+TGG - chr8_2:59134160-59134179 Msa1258040:intron 25.0%
!! TTTAGCTCAATAATTCAACT+GGG - chr8_2:59134161-59134180 Msa1258040:intron 25.0%
! ACAATCTATAATTGTAGTAG+AGG + chr8_2:59134422-59134441 None:intergenic 25.0%
! GAACTACCAAATTGAAAATT+GGG + chr8_2:59134923-59134942 None:intergenic 25.0%
! AGAACTACCAAATTGAAAAT+TGG + chr8_2:59134924-59134943 None:intergenic 25.0%
! TCTAGTATAATAAAGCCATA+AGG - chr8_2:59135010-59135029 Msa1258040:intron 25.0%
! ACAAAAACTAAACCAATTTG+TGG + chr8_2:59135036-59135055 None:intergenic 25.0%
! TTAAAAAAATCGAACCAATC+AGG - chr8_2:59135158-59135177 Msa1258040:intron 25.0%
! TATAAATCAAACCATGACAT+GGG + chr8_2:59135215-59135234 None:intergenic 25.0%
! TTATAAATCAAACCATGACA+TGG + chr8_2:59135216-59135235 None:intergenic 25.0%
!! ATAAATATCACTTCTTTTGC+TGG + chr8_2:59135273-59135292 None:intergenic 25.0%
! TACAAAAATTGAGATGATGA+TGG + chr8_2:59135367-59135386 None:intergenic 25.0%
!! CAATAGCTGTAATTGTATTT+TGG - chr8_2:59135445-59135464 Msa1258040:intron 25.0%
! AATACAAAGCAGTATCAAAA+GGG + chr8_2:59135875-59135894 None:intergenic 25.0%
! AAATACAAAGCAGTATCAAA+AGG + chr8_2:59135876-59135895 None:intergenic 25.0%
!! AACTTTTATGCTGATATAGA+TGG - chr8_2:59135910-59135929 Msa1258040:intron 25.0%
! TAATAACAAATACTGTTACC+AGG + chr8_2:59136071-59136090 None:intergenic 25.0%
!!! TATTTGTTATTACTCTGATC+AGG - chr8_2:59136079-59136098 Msa1258040:CDS 25.0%
! ATGCAAGAATTGTAAGTTAA+TGG + chr8_2:59136271-59136290 None:intergenic 25.0%
! TCAAAAACAACTACTCATAA+GGG + chr8_2:59136330-59136349 None:intergenic 25.0%
! ATCAAAAACAACTACTCATA+AGG + chr8_2:59136331-59136350 None:intergenic 25.0%
GAAAAATCACAAGTTAGAGA+AGG - chr8_2:59133976-59133995 Msa1258040:three_prime_UTR 30.0%
GGATTACAATTAAGGCAATA+AGG + chr8_2:59134043-59134062 None:intergenic 30.0%
AGAGTTTGACCTGATAAATA+AGG + chr8_2:59134064-59134083 None:intergenic 30.0%
TAAAAAAGGGGGAAAAGAAT+GGG + chr8_2:59134098-59134117 None:intergenic 30.0%
ATAAAAAAGGGGGAAAAGAA+TGG + chr8_2:59134099-59134118 None:intergenic 30.0%
ATTATTTCAGTTAATGTGCC+TGG - chr8_2:59134248-59134267 Msa1258040:CDS 30.0%
TGCATCTCGAATAAACAAAA+TGG - chr8_2:59134308-59134327 Msa1258040:intron 30.0%
CTATAATTGTAGTAGAGGTA+AGG + chr8_2:59134417-59134436 None:intergenic 30.0%
AATTAGTCATTGTTTGCACT+CGG - chr8_2:59134544-59134563 Msa1258040:intron 30.0%
! GGTAGTTTGAGTTAGAAATT+TGG - chr8_2:59134581-59134600 Msa1258040:intron 30.0%
TGAGTTAGAAATTTGGATCT+TGG - chr8_2:59134588-59134607 Msa1258040:intron 30.0%
ATTTGGATCTTGGCATTATT+CGG - chr8_2:59134598-59134617 Msa1258040:intron 30.0%
! GATTCTAAGAAACTTCTTCA+CGG - chr8_2:59134776-59134795 Msa1258040:intron 30.0%
! ATTCTAAGAAACTTCTTCAC+GGG - chr8_2:59134777-59134796 Msa1258040:intron 30.0%
! TTGAAAGAATTTTGTGTCTC+TGG - chr8_2:59134837-59134856 Msa1258040:intron 30.0%
! GTTCAACCCAATTTTCAATT+TGG - chr8_2:59134914-59134933 Msa1258040:intron 30.0%
!!! TCTTTTTCCACTTTGTTGTT+TGG - chr8_2:59134941-59134960 Msa1258040:intron 30.0%
AAGATAACCAAACAACAAAG+TGG + chr8_2:59134951-59134970 None:intergenic 30.0%
!! ACTTTGTTGTTTGGTTATCT+TGG - chr8_2:59134950-59134969 Msa1258040:intron 30.0%
!!! TTGGTTTGGTTTGATTTTCT+GGG - chr8_2:59134969-59134988 Msa1258040:intron 30.0%
GTAGTACTCAGTTTATGTAA+AGG - chr8_2:59135107-59135126 Msa1258040:intron 30.0%
!! ATTTTTGTTTCATGCACCAA+GGG + chr8_2:59135413-59135432 None:intergenic 30.0%
TACAATTACAGCTATTGCAT+TGG + chr8_2:59135442-59135461 None:intergenic 30.0%
!! GTTTGTTGTAATTGCTAAGT+AGG - chr8_2:59135644-59135663 Msa1258040:intron 30.0%
! TAAATCTCATCCATGTTTTC+TGG - chr8_2:59135696-59135715 Msa1258040:intron 30.0%
! AAATCTCATCCATGTTTTCT+GGG - chr8_2:59135697-59135716 Msa1258040:intron 30.0%
!! CAACAACAATAGGTCATTTT+CGG + chr8_2:59135757-59135776 None:intergenic 30.0%
ACCATGATAGCTTAATAAAC+TGG - chr8_2:59136028-59136047 Msa1258040:CDS 30.0%
TCCAGTTTATTAAGCTATCA+TGG + chr8_2:59136032-59136051 None:intergenic 30.0%
TCAAGTCACTTACCAAAATA+AGG + chr8_2:59136217-59136236 None:intergenic 30.0%
TTGGTAAGTGACTTGAAAAA+AGG - chr8_2:59136221-59136240 Msa1258040:intron 30.0%
ACTTACAATTCTTGCATTTG+CGG - chr8_2:59136274-59136293 Msa1258040:intron 30.0%
!!! GTTTTTGATTGCTTGTTTTG+TGG - chr8_2:59136342-59136361 Msa1258040:CDS 30.0%
CCATAATAGACGTCAAATAT+GGG + chr8_2:59136424-59136443 None:intergenic 30.0%
TCCATAATAGACGTCAAATA+TGG + chr8_2:59136425-59136444 None:intergenic 30.0%
TATTATCCAACAACAGAAAC+CGG + chr8_2:59136480-59136499 None:intergenic 30.0%
AGTTTAACGATATACAGACA+CGG + chr8_2:59136509-59136528 None:intergenic 30.0%
AATCAGATTTATTACCTGTG+TGG - chr8_2:59136531-59136550 Msa1258040:five_prime_UTR 30.0%
TCCTGAGGTACAATACAATT+CGG + chr8_2:59133828-59133847 None:intergenic 35.0%
ATTCGAGATGCAGAATCATA+TGG + chr8_2:59134300-59134319 None:intergenic 35.0%
ACTTTATAGCGCTATCAAAC+TGG + chr8_2:59134690-59134709 None:intergenic 35.0%
! TTCTAAGAAACTTCTTCACG+GGG - chr8_2:59134778-59134797 Msa1258040:intron 35.0%
TTCATGTGTGATCAGTAGTT+TGG - chr8_2:59134806-59134825 Msa1258040:intron 35.0%
TCATGTGTGATCAGTAGTTT+GGG - chr8_2:59134807-59134826 Msa1258040:intron 35.0%
GTTGTTTGGTTATCTTGGTT+TGG - chr8_2:59134955-59134974 Msa1258040:intron 35.0%
!!! CTTGGTTTGGTTTGATTTTC+TGG - chr8_2:59134968-59134987 Msa1258040:intron 35.0%
!!! TGGTTTGGTTTGATTTTCTG+GGG - chr8_2:59134970-59134989 Msa1258040:intron 35.0%
! TAAACCAATTTGTGGCCTTA+TGG + chr8_2:59135028-59135047 None:intergenic 35.0%
AGAAGATCTAGAAAAGAGTG+AGG + chr8_2:59135064-59135083 None:intergenic 35.0%
TTTAGTAAGATCCCATGTCA+TGG - chr8_2:59135201-59135220 Msa1258040:intron 35.0%
!! CATTTTTGTTTCATGCACCA+AGG + chr8_2:59135414-59135433 None:intergenic 35.0%
! CTTGGTGCATGAAACAAAAA+TGG - chr8_2:59135412-59135431 Msa1258040:intron 35.0%
TGCACTTCTTCACAATATCA+AGG + chr8_2:59135532-59135551 None:intergenic 35.0%
!!! ACAGTTTTGGATGTGTTCAT+TGG - chr8_2:59135669-59135688 Msa1258040:intron 35.0%
! AATCTCATCCATGTTTTCTG+GGG - chr8_2:59135698-59135717 Msa1258040:intron 35.0%
AAACTGGAATGTGCTTAGTA+TGG - chr8_2:59136044-59136063 Msa1258040:CDS 35.0%
!! TCCTATTCCTCTCCTTATTT+TGG - chr8_2:59136202-59136221 Msa1258040:intron 35.0%
ACCAAAATAAGGAGAGGAAT+AGG + chr8_2:59136206-59136225 None:intergenic 35.0%
TCACTTACCAAAATAAGGAG+AGG + chr8_2:59136212-59136231 None:intergenic 35.0%
!! TTGTTTTGTGGAATGCTTTC+TGG - chr8_2:59136354-59136373 Msa1258040:CDS 35.0%
TACTCTCATTTGCACATGTA+TGG + chr8_2:59136390-59136409 None:intergenic 35.0%
CCCATATTTGACGTCTATTA+TGG - chr8_2:59136421-59136440 Msa1258040:five_prime_UTR 35.0%
! TATGGACTAACTTCTACTCT+AGG - chr8_2:59136439-59136458 Msa1258040:five_prime_UTR 35.0%
TTAACGATCCACTTCACACA+AGG + chr8_2:59133771-59133790 None:intergenic 40.0%
GTGAAGTGGATCGTTAACAT+TGG - chr8_2:59133774-59133793 Msa1258040:three_prime_UTR 40.0%
AGTGGATCGTTAACATTGGT+TGG - chr8_2:59133778-59133797 Msa1258040:three_prime_UTR 40.0%
TCCGAATTGTATTGTACCTC+AGG - chr8_2:59133824-59133843 Msa1258040:three_prime_UTR 40.0%
CACAAGTTAGAGAAGGAGTA+TGG - chr8_2:59133983-59134002 Msa1258040:three_prime_UTR 40.0%
GTATGGCTGATATATTCGCT+TGG - chr8_2:59134000-59134019 Msa1258040:three_prime_UTR 40.0%
TAAGGCAATAAGGGTGAAGA+AGG + chr8_2:59134033-59134052 None:intergenic 40.0%
!! GGGGAAAAGAATGGGTTAAA+GGG + chr8_2:59134090-59134109 None:intergenic 40.0%
AATCAAATTCAAGGTCTGCC+AGG + chr8_2:59134269-59134288 None:intergenic 40.0%
CACTCGGATTTATGAGTGAA+TGG - chr8_2:59134560-59134579 Msa1258040:intron 40.0%
TAGCGCTATAAAGTACGTGT+TGG - chr8_2:59134696-59134715 Msa1258040:intron 40.0%
!! ACGTGTTGGTTGATGTTTCA+TGG - chr8_2:59134710-59134729 Msa1258040:intron 40.0%
AAAGCCATAAGGCCACAAAT+TGG - chr8_2:59135021-59135040 Msa1258040:intron 40.0%
! TAATCTGAGTACTGCTGCTA+GGG + chr8_2:59135305-59135324 None:intergenic 40.0%
! TTAATCTGAGTACTGCTGCT+AGG + chr8_2:59135306-59135325 None:intergenic 40.0%
! GTCAAGTTTGGTTCAGTTTG+AGG + chr8_2:59135345-59135364 None:intergenic 40.0%
!! GAGATGATGATGGTCAAGTT+TGG + chr8_2:59135357-59135376 None:intergenic 40.0%
! GGTATTGTCACTTCTTTCGT+TGG + chr8_2:59135392-59135411 None:intergenic 40.0%
GAAAGAAGTGACAATACCCT+TGG - chr8_2:59135394-59135413 Msa1258040:intron 40.0%
! TGCTAAGTAGGCTACAGTTT+TGG - chr8_2:59135656-59135675 Msa1258040:intron 40.0%
! ATCTCATCCATGTTTTCTGG+GGG - chr8_2:59135699-59135718 Msa1258040:intron 40.0%
AGAGTTGCTGCAACAACAAT+AGG + chr8_2:59135767-59135786 None:intergenic 40.0%
AGATATTCAACATGCTCCCT+TGG - chr8_2:59136136-59136155 Msa1258040:intron 40.0%
GATATTCAACATGCTCCCTT+GGG - chr8_2:59136137-59136156 Msa1258040:intron 40.0%
!! CAGGTTGATCTACACATCAT+TGG - chr8_2:59136300-59136319 Msa1258040:intron 40.0%
ACTAACTTCTACTCTAGGTG+TGG - chr8_2:59136444-59136463 Msa1258040:five_prime_UTR 40.0%
CTAACTTCTACTCTAGGTGT+GGG - chr8_2:59136445-59136464 Msa1258040:five_prime_UTR 40.0%
ACAGAAGAAGGAAACCTACG+AGG + chr8_2:59133862-59133881 None:intergenic 45.0%
!! GGGGGAAAAGAATGGGTTAA+AGG + chr8_2:59134091-59134110 None:intergenic 45.0%
GCAGCTACTGTGATATACTC+AGG + chr8_2:59134339-59134358 None:intergenic 45.0%
TACAAGGTAGAAACCGCACA+GGG + chr8_2:59134361-59134380 None:intergenic 45.0%
TTACAAGGTAGAAACCGCAC+AGG + chr8_2:59134362-59134381 None:intergenic 45.0%
TGCGGTTTCTACCTTGTAAC+AGG - chr8_2:59134363-59134382 Msa1258040:intron 45.0%
TCTACCTTGTAACAGGACAC+TGG - chr8_2:59134370-59134389 Msa1258040:intron 45.0%
CTACCTTGTAACAGGACACT+GGG - chr8_2:59134371-59134390 Msa1258040:intron 45.0%
TAACCCAGTGTCCTGTTACA+AGG + chr8_2:59134377-59134396 None:intergenic 45.0%
TGTAACAGGACACTGGGTTA+TGG - chr8_2:59134377-59134396 Msa1258040:intron 45.0%
TGTGATCAGTAGTTTGGGTG+AGG - chr8_2:59134812-59134831 Msa1258040:intron 45.0%
GTGATCAGTAGTTTGGGTGA+GGG - chr8_2:59134813-59134832 Msa1258040:intron 45.0%
CCTGCACTCGAGTTATAAAG+CGG + chr8_2:59134869-59134888 None:intergenic 45.0%
! AATCTGAGTACTGCTGCTAG+GGG + chr8_2:59135304-59135323 None:intergenic 45.0%
GATGCGACTGCAATTGATCA+TGG - chr8_2:59135565-59135584 Msa1258040:intron 45.0%
CATATCTCCCCCAGAAAACA+TGG + chr8_2:59135709-59135728 None:intergenic 45.0%
GAATGTGCTTAGTATGGTCC+TGG - chr8_2:59136050-59136069 Msa1258040:CDS 45.0%
ACTCTGATCAGGTACAGTCA+AGG - chr8_2:59136090-59136109 Msa1258040:CDS 45.0%
!! GTACAGTCAAGGAAAGCATC+TGG - chr8_2:59136101-59136120 Msa1258040:CDS 45.0%
TCCCTTGGGAAAAGAAGCAA+CGG - chr8_2:59136151-59136170 Msa1258040:intron 45.0%
AAGGAAACCTACGAGGATCG+AGG + chr8_2:59133855-59133874 None:intergenic 50.0%
GCACGGAGAAGAACAGAAGA+AGG + chr8_2:59133874-59133893 None:intergenic 50.0%
CCGCTTTATAACTCGAGTGC+AGG - chr8_2:59134866-59134885 Msa1258040:intron 50.0%
ATCGAACCAATCAGGAGCAG+TGG - chr8_2:59135166-59135185 Msa1258040:intron 50.0%
ACTGAACCACTGCTCCTGAT+TGG + chr8_2:59135175-59135194 None:intergenic 50.0%
! ATCTGAGTACTGCTGCTAGG+GGG + chr8_2:59135303-59135322 None:intergenic 50.0%
TTGAGGGGACTGTGCTTCTA+TGG + chr8_2:59135803-59135822 None:intergenic 50.0%
! GCCGTTGCTTCTTTTCCCAA+GGG + chr8_2:59136155-59136174 None:intergenic 50.0%
! AGCCGTTGCTTCTTTTCCCA+AGG + chr8_2:59136156-59136175 None:intergenic 50.0%
ATTCTTGCATTTGCGGCTGC+AGG - chr8_2:59136281-59136300 Msa1258040:intron 50.0%
CCAACAACAGAAACCGGCAA+TGG + chr8_2:59136474-59136493 None:intergenic 50.0%
!! CCATTGCCGGTTTCTGTTGT+TGG - chr8_2:59136471-59136490 Msa1258040:five_prime_UTR 50.0%
TGAGAAAACACGAGCCACAC+AGG + chr8_2:59136548-59136567 None:intergenic 50.0%
GAAACCTACGAGGATCGAGG+AGG + chr8_2:59133852-59133871 None:intergenic 55.0%
TGAGGGAATTGTGCGCAGCA+CGG + chr8_2:59133891-59133910 None:intergenic 55.0%
GCGCACGATAGCAGCTATGA+GGG + chr8_2:59133908-59133927 None:intergenic 55.0%
TGCGCACGATAGCAGCTATG+AGG + chr8_2:59133909-59133928 None:intergenic 55.0%
ATCACAGTAGCTGCCCTGTG+CGG - chr8_2:59134345-59134364 Msa1258040:intron 55.0%
! TAGGTGTGGGTTGCCATTGC+CGG - chr8_2:59136458-59136477 Msa1258040:five_prime_UTR 55.0%
GAGGATCGAGGAGGCTCCTG+AGG + chr8_2:59133843-59133862 None:intergenic 65.0%
GGAGCCTCCTCGATCCTCGT+AGG - chr8_2:59133845-59133864 Msa1258040:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 59133765 59136645 59133765 ID=Msa1258040;Name=Msa1258040
chr8_2 mRNA 59133765 59136645 59133765 ID=Msa1258040-mRNA-1;Parent=Msa1258040;Name=Msa1258040-mRNA-1;_AED=0.03;_eAED=0.03;_QI=237|1|1|1|1|1|4|306|137
chr8_2 exon 59133765 59134107 59133765 ID=Msa1258040-mRNA-1:exon:18331;Parent=Msa1258040-mRNA-1
chr8_2 exon 59134187 59134309 59134187 ID=Msa1258040-mRNA-1:exon:18330;Parent=Msa1258040-mRNA-1
chr8_2 exon 59135971 59136152 59135971 ID=Msa1258040-mRNA-1:exon:18329;Parent=Msa1258040-mRNA-1
chr8_2 exon 59136337 59136645 59136337 ID=Msa1258040-mRNA-1:exon:18328;Parent=Msa1258040-mRNA-1
chr8_2 five_prime_UTR 59136409 59136645 59136409 ID=Msa1258040-mRNA-1:five_prime_utr;Parent=Msa1258040-mRNA-1
chr8_2 CDS 59136337 59136408 59136337 ID=Msa1258040-mRNA-1:cds;Parent=Msa1258040-mRNA-1
chr8_2 CDS 59135971 59136152 59135971 ID=Msa1258040-mRNA-1:cds;Parent=Msa1258040-mRNA-1
chr8_2 CDS 59134187 59134309 59134187 ID=Msa1258040-mRNA-1:cds;Parent=Msa1258040-mRNA-1
chr8_2 CDS 59134071 59134107 59134071 ID=Msa1258040-mRNA-1:cds;Parent=Msa1258040-mRNA-1
chr8_2 three_prime_UTR 59133765 59134070 59133765 ID=Msa1258040-mRNA-1:three_prime_utr;Parent=Msa1258040-mRNA-1
Gene Sequence

>Msa1258040

ATGGCTGATATATTCGCTTGGCTCTTCTCCTTCTTCACCCTTATTGCCTTAATTGTAATCCTTATTTATCAGTTAATGTGCCTGGCAGACCTTGAATTTGATTATATAAATCCATATGATTCTGCATCTCGAATAAACAAAATGGTTATGCCTGAGTATATCACAGTAGCTGCCCTGTGCGGTTTCTACCTTGTAACAGGACACTGGGTTATGGCACTCTTGTGTGCTCCTTACCTCTACTACAATTATAGATTGTACAGTCAAGGAAAGCATCTGGTTGATGTTACAGAGATATTCAACATGCTCCCTTGGGAAAAGAAGCAACGGCTTTTCAAACTCTTCTATCTGATATTTATCCTATTCCTCTCCTTATTTTGGTTGATCTACACATCATTGGACGACCCTTATGAGTAG
Protein sequence

>Msa1258040

MADIFAWLFSFFTLIALIVILIYQLMCLADLEFDYINPYDSASRINKMVMPEYITVAALCGFYLVTGHWVMALLCAPYLYYNYRLYSQGKHLVDVTEIFNMLPWEKKQRLFKLFYLIFILFLSLFWLIYTSLDDPYE*