AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1401690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1401690 MtrunA17_Chr4g0056711 95.652 253 6 1 1 248 1 253 9.62e-176 485
Msa1401690 MtrunA17_Chr5g0398451 62.637 182 68 0 67 248 77 258 1.04e-85 258
Msa1401690 MtrunA17_Chr2g0291431 42.593 216 121 2 33 248 25 237 4.18e-60 192
Msa1401690 MtrunA17_Chr3g0109441 56.452 62 27 0 187 248 1 62 9.32e-21 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1401690 AT1G12450.1 62.500 232 84 3 17 248 11 239 8.73e-102 298
Msa1401690 AT4G22850.1 62.162 222 80 1 27 248 19 236 1.83e-92 274
Msa1401690 AT4G22850.2 62.162 222 80 1 27 248 19 236 2.77e-92 273
Msa1401690 AT4G12000.1 53.333 240 108 2 13 248 6 245 2.67e-86 259
Msa1401690 AT4G12000.2 53.333 240 108 2 13 248 6 245 2.67e-86 259
Msa1401690 AT4G12000.3 53.333 240 108 2 13 248 6 245 2.67e-86 259
Msa1401690 AT2G02370.2 39.149 235 138 3 14 248 6 235 5.06e-61 195
Msa1401690 AT2G02370.1 39.149 235 138 3 14 248 6 235 5.06e-61 195
Msa1401690 AT2G02370.3 39.149 235 138 3 14 248 6 235 5.06e-61 195

Find 98 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGTTGTTTCTGAGTTTA+GGG 0.181599 tig0022713:+98055 Msa1401690:CDS
AAGTACGGGCCTTACATAAT+TGG 0.221111 tig0022713:+98870 Msa1401690:CDS
TCTGTCTCCCAATTTATTAT+TGG 0.230391 tig0022713:-98407 None:intergenic
GTTCTGTTGTTTCTGAGTTT+AGG 0.232102 tig0022713:+98054 Msa1401690:CDS
GCGAATATAGGTTATGTAAT+TGG 0.238373 tig0022713:+99080 Msa1401690:three_prime_UTR
TAGATTGCAACAAATATTTC+TGG 0.242638 tig0022713:-98912 None:intergenic
TTACTTTATGCCTTCATATA+TGG 0.243841 tig0022713:+99152 Msa1401690:three_prime_UTR
CAATAATTGAAGACCATATA+AGG 0.250509 tig0022713:-98828 None:intergenic
AAGATTGAGCCAATGATAAA+AGG 0.256065 tig0022713:-98596 None:intergenic
TGACACTTGGTTATGGATTT+GGG 0.272523 tig0022713:+98531 Msa1401690:CDS
AGGTGATTCCAATAATAAAT+TGG 0.276150 tig0022713:+98399 Msa1401690:CDS
TTTAGAATAGAAGCCTTCTT+TGG 0.281924 tig0022713:-98738 None:intergenic
TGAGTATGTTTAGCAGAATA+TGG 0.288697 tig0022713:+99106 Msa1401690:three_prime_UTR
GTGACACTTGGTTATGGATT+TGG 0.289964 tig0022713:+98530 Msa1401690:CDS
GACGATCCGTCGTGTGGTAT+TGG 0.309310 tig0022713:+98022 Msa1401690:CDS
GATTTCTCCGTTCCCTTATA+TGG 0.318092 tig0022713:+98815 Msa1401690:CDS
TGGCTGTTGTTGTTCTCAAA+TGG 0.320051 tig0022713:+98082 Msa1401690:CDS
TTAGAGCTGTTGCCTTAATC+AGG 0.322826 tig0022713:+98793 Msa1401690:CDS
AGAAACCTTGTCAATGAGAT+AGG 0.333314 tig0022713:-98112 None:intergenic
ACGATCCGTCGTGTGGTATT+GGG 0.356797 tig0022713:+98023 Msa1401690:CDS
CGGTGGCGGTGGATGGAAAA+AGG 0.357415 tig0022713:+97861 Msa1401690:CDS
GCTGGAGTGACACTTGGTTA+TGG 0.359406 tig0022713:+98524 Msa1401690:CDS
AATCTGCTGGTGCAGGAAAC+TGG 0.369463 tig0022713:+98760 Msa1401690:CDS
ACTTTCTCCACCACAGTTAA+CGG 0.372710 tig0022713:-97976 None:intergenic
CTATGCCATACTGCGAATAT+AGG 0.377604 tig0022713:+99068 Msa1401690:three_prime_UTR
TATGAACGGTTGATCATGTT+TGG 0.381657 tig0022713:-97757 None:intergenic
GAACGGAGAAATCCTGATTA+AGG 0.382308 tig0022713:-98805 None:intergenic
ATAATAACTGCAGCAGCTAT+TGG 0.391137 tig0022713:+98563 Msa1401690:CDS
GGCTGTTGTTGTTCTCAAAT+GGG 0.411976 tig0022713:+98083 Msa1401690:CDS
CATAACCTATATTCGCAGTA+TGG 0.412858 tig0022713:-99073 None:intergenic
CATGTTTGGTTTAAGTGAAT+CGG 0.416917 tig0022713:-97743 None:intergenic
AAGAAGAGATTAATGAATAA+TGG 0.418233 tig0022713:-97687 None:intergenic
GCCTTACATAATTGGATCCT+TGG 0.420933 tig0022713:+98878 Msa1401690:CDS
AACTCCGGTGACGGTGGCGG+TGG 0.422523 tig0022713:+97850 Msa1401690:CDS
GTCTTGCAGACTCTTCATCT+TGG 0.437439 tig0022713:+99239 Msa1401690:three_prime_UTR
TGCCATCTACACCCTCTATG+TGG 0.439100 tig0022713:+98498 Msa1401690:CDS
GTAGATGGCAAAAGTATTGT+TGG 0.444313 tig0022713:-98485 None:intergenic
GGTGATTCCAATAATAAATT+GGG 0.444656 tig0022713:+98400 Msa1401690:CDS
CGTGTGGTATTGGGTCAAAA+TGG 0.449991 tig0022713:+98032 Msa1401690:CDS
CCATCCACCGCCACCGTCAC+CGG 0.465993 tig0022713:-97854 None:intergenic
ACCCACATAGAGGGTGTAGA+TGG 0.470244 tig0022713:-98500 None:intergenic
GACAGAGACTTTCAGTCCTC+CGG 0.473432 tig0022713:+98424 Msa1401690:CDS
AAGCTGATGATGATAACAAA+GGG 0.478409 tig0022713:+97914 Msa1401690:CDS
CCGGTGACGGTGGCGGTGGA+TGG 0.482219 tig0022713:+97854 Msa1401690:CDS
GTTGTTGTTCTCAAATGGGT+TGG 0.483636 tig0022713:+98087 Msa1401690:CDS
AGATTGAGCCAATGATAAAA+GGG 0.483710 tig0022713:-98595 None:intergenic
ATTCTAAAATCTGCTGGTGC+AGG 0.484602 tig0022713:+98753 Msa1401690:CDS
ACATAATTGGATCCTTGGTC+GGG 0.486638 tig0022713:+98883 Msa1401690:CDS
GCTTCTATTCTAAAATCTGC+TGG 0.490012 tig0022713:+98747 Msa1401690:CDS
ACACCCTCTATGTGGGTGGC+TGG 0.496986 tig0022713:+98506 Msa1401690:CDS
GCCATCTACACCCTCTATGT+GGG 0.497988 tig0022713:+98499 Msa1401690:CDS
AATAATTGAAGACCATATAA+GGG 0.500714 tig0022713:-98827 None:intergenic
TACATAATTGGATCCTTGGT+CGG 0.503152 tig0022713:+98882 Msa1401690:CDS
ACCAAGGATCCAATTATGTA+AGG 0.506973 tig0022713:-98879 None:intergenic
GATTATATCAAATTGATGCC+TGG 0.514087 tig0022713:+97937 Msa1401690:CDS
GTGGCAACAAATGTTAAGTA+CGG 0.515467 tig0022713:+98855 Msa1401690:CDS
ACGCGTTTGGTGTGATTCGA+AGG 0.519349 tig0022713:-97647 None:intergenic
AGACATCAAAAGTAGGCAAA+AGG 0.520072 tig0022713:-99047 None:intergenic
GTTAACTGTGGTGGAGAAAG+TGG 0.528551 tig0022713:+97978 Msa1401690:CDS
CACACGACGGATCGTCTTGA+CGG 0.531902 tig0022713:-98015 None:intergenic
TACATTACTCGTGGTGGTGA+TGG 0.535558 tig0022713:+97709 Msa1401690:five_prime_UTR
TAGATGGCAAAAGTATTGTT+GGG 0.536380 tig0022713:-98484 None:intergenic
GATGATGAGAGAAACGCGTT+TGG 0.539678 tig0022713:-97660 None:intergenic
GCAACTTGTACCATATATGA+AGG 0.541863 tig0022713:-99162 None:intergenic
GATAACTCCGGTGACGGTGG+CGG 0.546344 tig0022713:+97847 Msa1401690:CDS
GTTAGAGAAGTATCCAAAGA+AGG 0.550303 tig0022713:+98725 Msa1401690:CDS
AATTGGATCCTTGGTCGGGA+TGG 0.551661 tig0022713:+98887 Msa1401690:CDS
ATCTCTTCTTACATTACTCG+TGG 0.557425 tig0022713:+97700 Msa1401690:five_prime_UTR
GAAGCTGATGATGATAACAA+AGG 0.566873 tig0022713:+97913 Msa1401690:CDS
TGGCAACAAATGTTAAGTAC+GGG 0.566897 tig0022713:+98856 Msa1401690:CDS
ATGGCATAGACATCAAAAGT+AGG 0.574878 tig0022713:-99054 None:intergenic
TGGGTGGCTGGAGTGACACT+TGG 0.580051 tig0022713:+98518 Msa1401690:CDS
GAAACCTTGTCAATGAGATA+GGG 0.583464 tig0022713:-98111 None:intergenic
ATCTACACCCTCTATGTGGG+TGG 0.585631 tig0022713:+98502 Msa1401690:CDS
GGTAAACACTCATCGGAACC+AGG 0.589023 tig0022713:-97955 None:intergenic
TTCTGGCACCATCCCGACCA+AGG 0.594140 tig0022713:-98895 None:intergenic
AACGAGGAAACGTGAAAACA+TGG 0.596336 tig0022713:-99266 None:intergenic
AACAACAAAATAGTAAGCAC+CGG 0.597356 tig0022713:-98443 None:intergenic
TCACCGGAGTTATCCTTCAT+CGG 0.603801 tig0022713:-97838 None:intergenic
CACTCCAGCCACCCACATAG+AGG 0.605454 tig0022713:-98510 None:intergenic
ATTTGTTGCAATCTATACGT+AGG 0.611270 tig0022713:+98920 Msa1401690:CDS
TTGAAGACCATATAAGGGAA+CGG 0.611668 tig0022713:-98822 None:intergenic
AGTTAACGGTAAACACTCAT+CGG 0.614577 tig0022713:-97962 None:intergenic
ATGAAGGATAACTCCGGTGA+CGG 0.616962 tig0022713:+97841 Msa1401690:CDS
GTGTTTACCGTTAACTGTGG+TGG 0.623625 tig0022713:+97969 Msa1401690:CDS
CGTCAAGACGATCCGTCGTG+TGG 0.627835 tig0022713:+98016 Msa1401690:CDS
TGGACCCTATCTCATTGACA+AGG 0.629821 tig0022713:+98107 Msa1401690:CDS
TCTTCTTACATTACTCGTGG+TGG 0.631555 tig0022713:+97703 Msa1401690:five_prime_UTR
CATCAAAAGTAGGCAAAAGG+TGG 0.634613 tig0022713:-99044 None:intergenic
AAGGATAACTCCGGTGACGG+TGG 0.642097 tig0022713:+97844 Msa1401690:CDS
ACTCCAGCCACCCACATAGA+GGG 0.645848 tig0022713:-98509 None:intergenic
TTTCCGATGAAGGATAACTC+CGG 0.656857 tig0022713:+97835 Msa1401690:exon
GGTCTTCAATTATTGTGCTG+TGG 0.660894 tig0022713:+98836 Msa1401690:CDS
AACTGTGGTGGAGAAAGTGG+AGG 0.671574 tig0022713:+97981 Msa1401690:CDS
AACAAAATAGTAAGCACCGG+AGG 0.675222 tig0022713:-98440 None:intergenic
AGCTGATGATGATAACAAAG+GGG 0.714737 tig0022713:+97915 Msa1401690:CDS
TTTGACCCAATACCACACGA+CGG 0.720628 tig0022713:-98028 None:intergenic
TGAGTGTTTACCGTTAACTG+TGG 0.722193 tig0022713:+97966 Msa1401690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTTTTTTTATTATGAA+CGG - tig0022713:97774-97793 None:intergenic 10.0%
!!! AGATTTTTTTTTATTATGAA+CGG - tig0022713:97774-97793 None:intergenic 10.0%
!!! TTTTTTCTTTTTTCACTTTT+AGG + tig0022713:97793-97812 Msa1401690:five_prime_UTR 15.0%
!!! TTTTTCTTTTTTCACTTTTA+GGG + tig0022713:97794-97813 Msa1401690:five_prime_UTR 15.0%
!!! CAATTGTTTGTTTTTATATT+TGG + tig0022713:98148-98167 Msa1401690:intron 15.0%
!!! TAAAAAACAGAAATTTTCAA+CGG - tig0022713:98202-98221 None:intergenic 15.0%
!! TCTCAATTAATTTAATGTAT+TGG + tig0022713:98318-98337 Msa1401690:intron 15.0%
!!! TTAATTTAATGTATTGGTTA+TGG + tig0022713:98324-98343 Msa1401690:intron 15.0%
!!! TTGCATAATTTTAATTTTTC+AGG + tig0022713:98376-98395 Msa1401690:intron 15.0%
!!! TTTTTTCTTTTTTCACTTTT+AGG + tig0022713:97793-97812 Msa1401690:five_prime_UTR 15.0%
!!! TTTTTCTTTTTTCACTTTTA+GGG + tig0022713:97794-97813 Msa1401690:five_prime_UTR 15.0%
!!! CAATTGTTTGTTTTTATATT+TGG + tig0022713:98148-98167 Msa1401690:intron 15.0%
!!! TAAAAAACAGAAATTTTCAA+CGG - tig0022713:98202-98221 None:intergenic 15.0%
!! TCTCAATTAATTTAATGTAT+TGG + tig0022713:98318-98337 Msa1401690:intron 15.0%
!!! TTAATTTAATGTATTGGTTA+TGG + tig0022713:98324-98343 Msa1401690:intron 15.0%
!!! TTGCATAATTTTAATTTTTC+AGG + tig0022713:98376-98395 Msa1401690:intron 15.0%
!! AAGAAGAGATTAATGAATAA+TGG - tig0022713:97690-97709 None:intergenic 20.0%
!!! TTTATATTTGGATTGTTTTG+TGG + tig0022713:98160-98179 Msa1401690:intron 20.0%
!!! CATAATTTTAATTTTTCAGG+AGG + tig0022713:98379-98398 Msa1401690:intron 20.0%
!! AATAATTGAAGACCATATAA+GGG - tig0022713:98830-98849 None:intergenic 20.0%
!!! TGAATTCATATCATTTTATG+TGG + tig0022713:98982-99001 Msa1401690:three_prime_UTR 20.0%
!! AAGAAGAGATTAATGAATAA+TGG - tig0022713:97690-97709 None:intergenic 20.0%
!!! TTTATATTTGGATTGTTTTG+TGG + tig0022713:98160-98179 Msa1401690:intron 20.0%
!!! CATAATTTTAATTTTTCAGG+AGG + tig0022713:98379-98398 Msa1401690:intron 20.0%
!! AATAATTGAAGACCATATAA+GGG - tig0022713:98830-98849 None:intergenic 20.0%
!!! TGAATTCATATCATTTTATG+TGG + tig0022713:98982-99001 Msa1401690:three_prime_UTR 20.0%
! TTGTGTCAATGAATATCTAA+TGG + tig0022713:98352-98371 Msa1401690:intron 25.0%
! TGTGTCAATGAATATCTAAT+GGG + tig0022713:98353-98372 Msa1401690:intron 25.0%
! AGGTGATTCCAATAATAAAT+TGG + tig0022713:98399-98418 Msa1401690:CDS 25.0%
! GGTGATTCCAATAATAAATT+GGG + tig0022713:98400-98419 Msa1401690:CDS 25.0%
!! CACTTACTTCAATTTTATGA+TGG - tig0022713:98622-98641 None:intergenic 25.0%
! CAATAATTGAAGACCATATA+AGG - tig0022713:98831-98850 None:intergenic 25.0%
! TAGATTGCAACAAATATTTC+TGG - tig0022713:98915-98934 None:intergenic 25.0%
! TTACTTTATGCCTTCATATA+TGG + tig0022713:99152-99171 Msa1401690:three_prime_UTR 25.0%
!! CTTCAGTTAGATTTCTTTTA+TGG + tig0022713:99184-99203 Msa1401690:three_prime_UTR 25.0%
!! ATTTTCTTGAAATCTAAACG+AGG - tig0022713:99285-99304 None:intergenic 25.0%
! TTGTGTCAATGAATATCTAA+TGG + tig0022713:98352-98371 Msa1401690:intron 25.0%
! TGTGTCAATGAATATCTAAT+GGG + tig0022713:98353-98372 Msa1401690:intron 25.0%
! AGGTGATTCCAATAATAAAT+TGG + tig0022713:98399-98418 Msa1401690:CDS 25.0%
! GGTGATTCCAATAATAAATT+GGG + tig0022713:98400-98419 Msa1401690:CDS 25.0%
!! CACTTACTTCAATTTTATGA+TGG - tig0022713:98622-98641 None:intergenic 25.0%
! CAATAATTGAAGACCATATA+AGG - tig0022713:98831-98850 None:intergenic 25.0%
! TAGATTGCAACAAATATTTC+TGG - tig0022713:98915-98934 None:intergenic 25.0%
! TTACTTTATGCCTTCATATA+TGG + tig0022713:99152-99171 Msa1401690:three_prime_UTR 25.0%
!! CTTCAGTTAGATTTCTTTTA+TGG + tig0022713:99184-99203 Msa1401690:three_prime_UTR 25.0%
!! ATTTTCTTGAAATCTAAACG+AGG - tig0022713:99285-99304 None:intergenic 25.0%
! CATGTTTGGTTTAAGTGAAT+CGG - tig0022713:97746-97765 None:intergenic 30.0%
! AAGCTGATGATGATAACAAA+GGG + tig0022713:97914-97933 Msa1401690:CDS 30.0%
GATTATATCAAATTGATGCC+TGG + tig0022713:97937-97956 Msa1401690:CDS 30.0%
!! TTCTGTTGTTTCTGAGTTTA+GGG + tig0022713:98055-98074 Msa1401690:CDS 30.0%
!! TGGATTGTTTTGTGGAATTT+AGG + tig0022713:98168-98187 Msa1401690:intron 30.0%
!! GGATTGTTTTGTGGAATTTA+GGG + tig0022713:98169-98188 Msa1401690:intron 30.0%
TCTGTCTCCCAATTTATTAT+TGG - tig0022713:98410-98429 None:intergenic 30.0%
AACAACAAAATAGTAAGCAC+CGG - tig0022713:98446-98465 None:intergenic 30.0%
!! TAGATGGCAAAAGTATTGTT+GGG - tig0022713:98487-98506 None:intergenic 30.0%
AGATTGAGCCAATGATAAAA+GGG - tig0022713:98598-98617 None:intergenic 30.0%
AAGATTGAGCCAATGATAAA+AGG - tig0022713:98599-98618 None:intergenic 30.0%
!! TGTGCCATTAAGATTTTGTA+TGG + tig0022713:98643-98662 Msa1401690:intron 30.0%
ATGTTTCATGAATCTATGCA+GGG + tig0022713:98699-98718 Msa1401690:intron 30.0%
! TTTAGAATAGAAGCCTTCTT+TGG - tig0022713:98741-98760 None:intergenic 30.0%
! ATTTGTTGCAATCTATACGT+AGG + tig0022713:98920-98939 Msa1401690:CDS 30.0%
AACAAAAATGATACGAGAGT+GGG - tig0022713:98956-98975 None:intergenic 30.0%
TAACAAAAATGATACGAGAG+TGG - tig0022713:98957-98976 None:intergenic 30.0%
GCGAATATAGGTTATGTAAT+TGG + tig0022713:99080-99099 Msa1401690:three_prime_UTR 30.0%
TGAGTATGTTTAGCAGAATA+TGG + tig0022713:99106-99125 Msa1401690:three_prime_UTR 30.0%
! CATGTTTGGTTTAAGTGAAT+CGG - tig0022713:97746-97765 None:intergenic 30.0%
! AAGCTGATGATGATAACAAA+GGG + tig0022713:97914-97933 Msa1401690:CDS 30.0%
GATTATATCAAATTGATGCC+TGG + tig0022713:97937-97956 Msa1401690:CDS 30.0%
!! TTCTGTTGTTTCTGAGTTTA+GGG + tig0022713:98055-98074 Msa1401690:CDS 30.0%
!! TGGATTGTTTTGTGGAATTT+AGG + tig0022713:98168-98187 Msa1401690:intron 30.0%
!! GGATTGTTTTGTGGAATTTA+GGG + tig0022713:98169-98188 Msa1401690:intron 30.0%
TCTGTCTCCCAATTTATTAT+TGG - tig0022713:98410-98429 None:intergenic 30.0%
AACAACAAAATAGTAAGCAC+CGG - tig0022713:98446-98465 None:intergenic 30.0%
!! TAGATGGCAAAAGTATTGTT+GGG - tig0022713:98487-98506 None:intergenic 30.0%
AGATTGAGCCAATGATAAAA+GGG - tig0022713:98598-98617 None:intergenic 30.0%
AAGATTGAGCCAATGATAAA+AGG - tig0022713:98599-98618 None:intergenic 30.0%
!! TGTGCCATTAAGATTTTGTA+TGG + tig0022713:98643-98662 Msa1401690:intron 30.0%
ATGTTTCATGAATCTATGCA+GGG + tig0022713:98699-98718 Msa1401690:intron 30.0%
! TTTAGAATAGAAGCCTTCTT+TGG - tig0022713:98741-98760 None:intergenic 30.0%
! ATTTGTTGCAATCTATACGT+AGG + tig0022713:98920-98939 Msa1401690:CDS 30.0%
AACAAAAATGATACGAGAGT+GGG - tig0022713:98956-98975 None:intergenic 30.0%
TAACAAAAATGATACGAGAG+TGG - tig0022713:98957-98976 None:intergenic 30.0%
GCGAATATAGGTTATGTAAT+TGG + tig0022713:99080-99099 Msa1401690:three_prime_UTR 30.0%
TGAGTATGTTTAGCAGAATA+TGG + tig0022713:99106-99125 Msa1401690:three_prime_UTR 30.0%
!!! GGTTTCGATTTTGTTGCTTT+TGG - tig0022713:97599-97618 None:intergenic 35.0%
!!! TGCAAGAAGCGCTTTTTTTT+GGG - tig0022713:97620-97639 None:intergenic 35.0%
!!! TTGCAAGAAGCGCTTTTTTT+TGG - tig0022713:97621-97640 None:intergenic 35.0%
ATCTCTTCTTACATTACTCG+TGG + tig0022713:97700-97719 Msa1401690:five_prime_UTR 35.0%
TATGAACGGTTGATCATGTT+TGG - tig0022713:97760-97779 None:intergenic 35.0%
! TTTTGTGCATTTTCCGATGA+AGG + tig0022713:97825-97844 Msa1401690:exon 35.0%
ATGGAAAAAGGAGGTTGTTT+CGG + tig0022713:97873-97892 Msa1401690:CDS 35.0%
! GAAGCTGATGATGATAACAA+AGG + tig0022713:97913-97932 Msa1401690:CDS 35.0%
! AGCTGATGATGATAACAAAG+GGG + tig0022713:97915-97934 Msa1401690:CDS 35.0%
AGTTAACGGTAAACACTCAT+CGG - tig0022713:97965-97984 None:intergenic 35.0%
!! GTTCTGTTGTTTCTGAGTTT+AGG + tig0022713:98054-98073 Msa1401690:CDS 35.0%
!! GTTTCTGAGTTTAGGGTTTT+TGG + tig0022713:98062-98081 Msa1401690:CDS 35.0%
GAAACCTTGTCAATGAGATA+GGG - tig0022713:98114-98133 None:intergenic 35.0%
AGAAACCTTGTCAATGAGAT+AGG - tig0022713:98115-98134 None:intergenic 35.0%
!! GTAGATGGCAAAAGTATTGT+TGG - tig0022713:98488-98507 None:intergenic 35.0%
TGACACTTGGTTATGGATTT+GGG + tig0022713:98531-98550 Msa1401690:CDS 35.0%
ATAATAACTGCAGCAGCTAT+TGG + tig0022713:98563-98582 Msa1401690:CDS 35.0%
! GCCATTAAGATTTTGTATGG+CGG + tig0022713:98646-98665 Msa1401690:intron 35.0%
ACCGCCATACAAAATCTTAA+TGG - tig0022713:98650-98669 None:intergenic 35.0%
TCGATTCATGTGCACTAATT+TGG + tig0022713:98671-98690 Msa1401690:intron 35.0%
GATGTTTCATGAATCTATGC+AGG + tig0022713:98698-98717 Msa1401690:intron 35.0%
!! GTTAGAGAAGTATCCAAAGA+AGG + tig0022713:98725-98744 Msa1401690:CDS 35.0%
!! GCTTCTATTCTAAAATCTGC+TGG + tig0022713:98747-98766 Msa1401690:CDS 35.0%
TTGAAGACCATATAAGGGAA+CGG - tig0022713:98825-98844 None:intergenic 35.0%
GTGGCAACAAATGTTAAGTA+CGG + tig0022713:98855-98874 Msa1401690:CDS 35.0%
TGGCAACAAATGTTAAGTAC+GGG + tig0022713:98856-98875 Msa1401690:CDS 35.0%
ACCAAGGATCCAATTATGTA+AGG - tig0022713:98882-98901 None:intergenic 35.0%
TACATAATTGGATCCTTGGT+CGG + tig0022713:98882-98901 Msa1401690:CDS 35.0%
AGACATCAAAAGTAGGCAAA+AGG - tig0022713:99050-99069 None:intergenic 35.0%
ATGGCATAGACATCAAAAGT+AGG - tig0022713:99057-99076 None:intergenic 35.0%
CATAACCTATATTCGCAGTA+TGG - tig0022713:99076-99095 None:intergenic 35.0%
GCAACTTGTACCATATATGA+AGG - tig0022713:99165-99184 None:intergenic 35.0%
!!! GGTTTCGATTTTGTTGCTTT+TGG - tig0022713:97599-97618 None:intergenic 35.0%
!!! TGCAAGAAGCGCTTTTTTTT+GGG - tig0022713:97620-97639 None:intergenic 35.0%
!!! TTGCAAGAAGCGCTTTTTTT+TGG - tig0022713:97621-97640 None:intergenic 35.0%
ATCTCTTCTTACATTACTCG+TGG + tig0022713:97700-97719 Msa1401690:five_prime_UTR 35.0%
TATGAACGGTTGATCATGTT+TGG - tig0022713:97760-97779 None:intergenic 35.0%
! TTTTGTGCATTTTCCGATGA+AGG + tig0022713:97825-97844 Msa1401690:exon 35.0%
ATGGAAAAAGGAGGTTGTTT+CGG + tig0022713:97873-97892 Msa1401690:CDS 35.0%
! GAAGCTGATGATGATAACAA+AGG + tig0022713:97913-97932 Msa1401690:CDS 35.0%
! AGCTGATGATGATAACAAAG+GGG + tig0022713:97915-97934 Msa1401690:CDS 35.0%
AGTTAACGGTAAACACTCAT+CGG - tig0022713:97965-97984 None:intergenic 35.0%
!! GTTCTGTTGTTTCTGAGTTT+AGG + tig0022713:98054-98073 Msa1401690:CDS 35.0%
!! GTTTCTGAGTTTAGGGTTTT+TGG + tig0022713:98062-98081 Msa1401690:CDS 35.0%
GAAACCTTGTCAATGAGATA+GGG - tig0022713:98114-98133 None:intergenic 35.0%
AGAAACCTTGTCAATGAGAT+AGG - tig0022713:98115-98134 None:intergenic 35.0%
!! GTAGATGGCAAAAGTATTGT+TGG - tig0022713:98488-98507 None:intergenic 35.0%
TGACACTTGGTTATGGATTT+GGG + tig0022713:98531-98550 Msa1401690:CDS 35.0%
ATAATAACTGCAGCAGCTAT+TGG + tig0022713:98563-98582 Msa1401690:CDS 35.0%
! GCCATTAAGATTTTGTATGG+CGG + tig0022713:98646-98665 Msa1401690:intron 35.0%
ACCGCCATACAAAATCTTAA+TGG - tig0022713:98650-98669 None:intergenic 35.0%
TCGATTCATGTGCACTAATT+TGG + tig0022713:98671-98690 Msa1401690:intron 35.0%
GATGTTTCATGAATCTATGC+AGG + tig0022713:98698-98717 Msa1401690:intron 35.0%
!! GTTAGAGAAGTATCCAAAGA+AGG + tig0022713:98725-98744 Msa1401690:CDS 35.0%
!! GCTTCTATTCTAAAATCTGC+TGG + tig0022713:98747-98766 Msa1401690:CDS 35.0%
TTGAAGACCATATAAGGGAA+CGG - tig0022713:98825-98844 None:intergenic 35.0%
GTGGCAACAAATGTTAAGTA+CGG + tig0022713:98855-98874 Msa1401690:CDS 35.0%
TGGCAACAAATGTTAAGTAC+GGG + tig0022713:98856-98875 Msa1401690:CDS 35.0%
ACCAAGGATCCAATTATGTA+AGG - tig0022713:98882-98901 None:intergenic 35.0%
TACATAATTGGATCCTTGGT+CGG + tig0022713:98882-98901 Msa1401690:CDS 35.0%
AGACATCAAAAGTAGGCAAA+AGG - tig0022713:99050-99069 None:intergenic 35.0%
ATGGCATAGACATCAAAAGT+AGG - tig0022713:99057-99076 None:intergenic 35.0%
CATAACCTATATTCGCAGTA+TGG - tig0022713:99076-99095 None:intergenic 35.0%
GCAACTTGTACCATATATGA+AGG - tig0022713:99165-99184 None:intergenic 35.0%
TCTTCTTACATTACTCGTGG+TGG + tig0022713:97703-97722 Msa1401690:five_prime_UTR 40.0%
TTTCCGATGAAGGATAACTC+CGG + tig0022713:97835-97854 Msa1401690:exon 40.0%
TGAGTGTTTACCGTTAACTG+TGG + tig0022713:97966-97985 Msa1401690:CDS 40.0%
ACTTTCTCCACCACAGTTAA+CGG - tig0022713:97979-97998 None:intergenic 40.0%
! TGGCTGTTGTTGTTCTCAAA+TGG + tig0022713:98082-98101 Msa1401690:CDS 40.0%
! GGCTGTTGTTGTTCTCAAAT+GGG + tig0022713:98083-98102 Msa1401690:CDS 40.0%
! GTTGTTGTTCTCAAATGGGT+TGG + tig0022713:98087-98106 Msa1401690:CDS 40.0%
!! AACAAAATAGTAAGCACCGG+AGG - tig0022713:98443-98462 None:intergenic 40.0%
GTGACACTTGGTTATGGATT+TGG + tig0022713:98530-98549 Msa1401690:CDS 40.0%
!! GTGTCACTCCCTTTTATCAT+TGG + tig0022713:98587-98606 Msa1401690:CDS 40.0%
TTCATGAATCTATGCAGGGA+TGG + tig0022713:98703-98722 Msa1401690:intron 40.0%
!! ATTCTAAAATCTGCTGGTGC+AGG + tig0022713:98753-98772 Msa1401690:CDS 40.0%
TTAGAGCTGTTGCCTTAATC+AGG + tig0022713:98793-98812 Msa1401690:CDS 40.0%
GAACGGAGAAATCCTGATTA+AGG - tig0022713:98808-98827 None:intergenic 40.0%
GATTTCTCCGTTCCCTTATA+TGG + tig0022713:98815-98834 Msa1401690:CDS 40.0%
GGTCTTCAATTATTGTGCTG+TGG + tig0022713:98836-98855 Msa1401690:CDS 40.0%
AAGTACGGGCCTTACATAAT+TGG + tig0022713:98870-98889 Msa1401690:CDS 40.0%
GCCTTACATAATTGGATCCT+TGG + tig0022713:98878-98897 Msa1401690:CDS 40.0%
ACATAATTGGATCCTTGGTC+GGG + tig0022713:98883-98902 Msa1401690:CDS 40.0%
CATCAAAAGTAGGCAAAAGG+TGG - tig0022713:99047-99066 None:intergenic 40.0%
CTATGCCATACTGCGAATAT+AGG + tig0022713:99068-99087 Msa1401690:three_prime_UTR 40.0%
AACGAGGAAACGTGAAAACA+TGG - tig0022713:99269-99288 None:intergenic 40.0%
TCTTCTTACATTACTCGTGG+TGG + tig0022713:97703-97722 Msa1401690:five_prime_UTR 40.0%
TTTCCGATGAAGGATAACTC+CGG + tig0022713:97835-97854 Msa1401690:exon 40.0%
TGAGTGTTTACCGTTAACTG+TGG + tig0022713:97966-97985 Msa1401690:CDS 40.0%
ACTTTCTCCACCACAGTTAA+CGG - tig0022713:97979-97998 None:intergenic 40.0%
! TGGCTGTTGTTGTTCTCAAA+TGG + tig0022713:98082-98101 Msa1401690:CDS 40.0%
! GGCTGTTGTTGTTCTCAAAT+GGG + tig0022713:98083-98102 Msa1401690:CDS 40.0%
! GTTGTTGTTCTCAAATGGGT+TGG + tig0022713:98087-98106 Msa1401690:CDS 40.0%
!! AACAAAATAGTAAGCACCGG+AGG - tig0022713:98443-98462 None:intergenic 40.0%
GTGACACTTGGTTATGGATT+TGG + tig0022713:98530-98549 Msa1401690:CDS 40.0%
!! GTGTCACTCCCTTTTATCAT+TGG + tig0022713:98587-98606 Msa1401690:CDS 40.0%
TTCATGAATCTATGCAGGGA+TGG + tig0022713:98703-98722 Msa1401690:intron 40.0%
!! ATTCTAAAATCTGCTGGTGC+AGG + tig0022713:98753-98772 Msa1401690:CDS 40.0%
TTAGAGCTGTTGCCTTAATC+AGG + tig0022713:98793-98812 Msa1401690:CDS 40.0%
GAACGGAGAAATCCTGATTA+AGG - tig0022713:98808-98827 None:intergenic 40.0%
GATTTCTCCGTTCCCTTATA+TGG + tig0022713:98815-98834 Msa1401690:CDS 40.0%
GGTCTTCAATTATTGTGCTG+TGG + tig0022713:98836-98855 Msa1401690:CDS 40.0%
AAGTACGGGCCTTACATAAT+TGG + tig0022713:98870-98889 Msa1401690:CDS 40.0%
GCCTTACATAATTGGATCCT+TGG + tig0022713:98878-98897 Msa1401690:CDS 40.0%
ACATAATTGGATCCTTGGTC+GGG + tig0022713:98883-98902 Msa1401690:CDS 40.0%
CATCAAAAGTAGGCAAAAGG+TGG - tig0022713:99047-99066 None:intergenic 40.0%
CTATGCCATACTGCGAATAT+AGG + tig0022713:99068-99087 Msa1401690:three_prime_UTR 40.0%
AACGAGGAAACGTGAAAACA+TGG - tig0022713:99269-99288 None:intergenic 40.0%
GATGATGAGAGAAACGCGTT+TGG - tig0022713:97663-97682 None:intergenic 45.0%
! TACATTACTCGTGGTGGTGA+TGG + tig0022713:97709-97728 Msa1401690:five_prime_UTR 45.0%
TCACCGGAGTTATCCTTCAT+CGG - tig0022713:97841-97860 None:intergenic 45.0%
ATGAAGGATAACTCCGGTGA+CGG + tig0022713:97841-97860 Msa1401690:CDS 45.0%
GTGTTTACCGTTAACTGTGG+TGG + tig0022713:97969-97988 Msa1401690:CDS 45.0%
! GTTAACTGTGGTGGAGAAAG+TGG + tig0022713:97978-97997 Msa1401690:CDS 45.0%
TTTGACCCAATACCACACGA+CGG - tig0022713:98031-98050 None:intergenic 45.0%
! CGTGTGGTATTGGGTCAAAA+TGG + tig0022713:98032-98051 Msa1401690:CDS 45.0%
TGGACCCTATCTCATTGACA+AGG + tig0022713:98107-98126 Msa1401690:CDS 45.0%
GTCTTGCAGACTCTTCATCT+TGG + tig0022713:99239-99258 Msa1401690:three_prime_UTR 45.0%
GATGATGAGAGAAACGCGTT+TGG - tig0022713:97663-97682 None:intergenic 45.0%
! TACATTACTCGTGGTGGTGA+TGG + tig0022713:97709-97728 Msa1401690:five_prime_UTR 45.0%
TCACCGGAGTTATCCTTCAT+CGG - tig0022713:97841-97860 None:intergenic 45.0%
ATGAAGGATAACTCCGGTGA+CGG + tig0022713:97841-97860 Msa1401690:CDS 45.0%
GTGTTTACCGTTAACTGTGG+TGG + tig0022713:97969-97988 Msa1401690:CDS 45.0%
! GTTAACTGTGGTGGAGAAAG+TGG + tig0022713:97978-97997 Msa1401690:CDS 45.0%
TTTGACCCAATACCACACGA+CGG - tig0022713:98031-98050 None:intergenic 45.0%
! CGTGTGGTATTGGGTCAAAA+TGG + tig0022713:98032-98051 Msa1401690:CDS 45.0%
TGGACCCTATCTCATTGACA+AGG + tig0022713:98107-98126 Msa1401690:CDS 45.0%
GTCTTGCAGACTCTTCATCT+TGG + tig0022713:99239-99258 Msa1401690:three_prime_UTR 45.0%
!! ACGCGTTTGGTGTGATTCGA+AGG - tig0022713:97650-97669 None:intergenic 50.0%
GGTAAACACTCATCGGAACC+AGG - tig0022713:97958-97977 None:intergenic 50.0%
! AACTGTGGTGGAGAAAGTGG+AGG + tig0022713:97981-98000 Msa1401690:CDS 50.0%
ACGATCCGTCGTGTGGTATT+GGG + tig0022713:98023-98042 Msa1401690:CDS 50.0%
GACAGAGACTTTCAGTCCTC+CGG + tig0022713:98424-98443 Msa1401690:CDS 50.0%
TGCCATCTACACCCTCTATG+TGG + tig0022713:98498-98517 Msa1401690:CDS 50.0%
GCCATCTACACCCTCTATGT+GGG + tig0022713:98499-98518 Msa1401690:CDS 50.0%
! ACCCACATAGAGGGTGTAGA+TGG - tig0022713:98503-98522 None:intergenic 50.0%
ATCTACACCCTCTATGTGGG+TGG + tig0022713:98502-98521 Msa1401690:CDS 50.0%
GCTGGAGTGACACTTGGTTA+TGG + tig0022713:98524-98543 Msa1401690:CDS 50.0%
AATCTGCTGGTGCAGGAAAC+TGG + tig0022713:98760-98779 Msa1401690:CDS 50.0%
AATTGGATCCTTGGTCGGGA+TGG + tig0022713:98887-98906 Msa1401690:CDS 50.0%
!! ACGCGTTTGGTGTGATTCGA+AGG - tig0022713:97650-97669 None:intergenic 50.0%
GGTAAACACTCATCGGAACC+AGG - tig0022713:97958-97977 None:intergenic 50.0%
! AACTGTGGTGGAGAAAGTGG+AGG + tig0022713:97981-98000 Msa1401690:CDS 50.0%
ACGATCCGTCGTGTGGTATT+GGG + tig0022713:98023-98042 Msa1401690:CDS 50.0%
GACAGAGACTTTCAGTCCTC+CGG + tig0022713:98424-98443 Msa1401690:CDS 50.0%
TGCCATCTACACCCTCTATG+TGG + tig0022713:98498-98517 Msa1401690:CDS 50.0%
GCCATCTACACCCTCTATGT+GGG + tig0022713:98499-98518 Msa1401690:CDS 50.0%
! ACCCACATAGAGGGTGTAGA+TGG - tig0022713:98503-98522 None:intergenic 50.0%
ATCTACACCCTCTATGTGGG+TGG + tig0022713:98502-98521 Msa1401690:CDS 50.0%
GCTGGAGTGACACTTGGTTA+TGG + tig0022713:98524-98543 Msa1401690:CDS 50.0%
AATCTGCTGGTGCAGGAAAC+TGG + tig0022713:98760-98779 Msa1401690:CDS 50.0%
AATTGGATCCTTGGTCGGGA+TGG + tig0022713:98887-98906 Msa1401690:CDS 50.0%
! AAGGATAACTCCGGTGACGG+TGG + tig0022713:97844-97863 Msa1401690:CDS 55.0%
TGGCGGTGGATGGAAAAAGG+AGG + tig0022713:97864-97883 Msa1401690:CDS 55.0%
CACACGACGGATCGTCTTGA+CGG - tig0022713:98018-98037 None:intergenic 55.0%
GACGATCCGTCGTGTGGTAT+TGG + tig0022713:98022-98041 Msa1401690:CDS 55.0%
ACTCCAGCCACCCACATAGA+GGG - tig0022713:98512-98531 None:intergenic 55.0%
! AAGGATAACTCCGGTGACGG+TGG + tig0022713:97844-97863 Msa1401690:CDS 55.0%
TGGCGGTGGATGGAAAAAGG+AGG + tig0022713:97864-97883 Msa1401690:CDS 55.0%
CACACGACGGATCGTCTTGA+CGG - tig0022713:98018-98037 None:intergenic 55.0%
GACGATCCGTCGTGTGGTAT+TGG + tig0022713:98022-98041 Msa1401690:CDS 55.0%
ACTCCAGCCACCCACATAGA+GGG - tig0022713:98512-98531 None:intergenic 55.0%
! GATAACTCCGGTGACGGTGG+CGG + tig0022713:97847-97866 Msa1401690:CDS 60.0%
! CGGTGGCGGTGGATGGAAAA+AGG + tig0022713:97861-97880 Msa1401690:CDS 60.0%
CGTCAAGACGATCCGTCGTG+TGG + tig0022713:98016-98035 Msa1401690:CDS 60.0%
ACACCCTCTATGTGGGTGGC+TGG + tig0022713:98506-98525 Msa1401690:CDS 60.0%
CACTCCAGCCACCCACATAG+AGG - tig0022713:98513-98532 None:intergenic 60.0%
TGGGTGGCTGGAGTGACACT+TGG + tig0022713:98518-98537 Msa1401690:CDS 60.0%
TTCTGGCACCATCCCGACCA+AGG - tig0022713:98898-98917 None:intergenic 60.0%
! GATAACTCCGGTGACGGTGG+CGG + tig0022713:97847-97866 Msa1401690:CDS 60.0%
! CGGTGGCGGTGGATGGAAAA+AGG + tig0022713:97861-97880 Msa1401690:CDS 60.0%
CGTCAAGACGATCCGTCGTG+TGG + tig0022713:98016-98035 Msa1401690:CDS 60.0%
ACACCCTCTATGTGGGTGGC+TGG + tig0022713:98506-98525 Msa1401690:CDS 60.0%
CACTCCAGCCACCCACATAG+AGG - tig0022713:98513-98532 None:intergenic 60.0%
TGGGTGGCTGGAGTGACACT+TGG + tig0022713:98518-98537 Msa1401690:CDS 60.0%
TTCTGGCACCATCCCGACCA+AGG - tig0022713:98898-98917 None:intergenic 60.0%
! AACTCCGGTGACGGTGGCGG+TGG + tig0022713:97850-97869 Msa1401690:CDS 70.0%
CCATCCACCGCCACCGTCAC+CGG - tig0022713:97857-97876 None:intergenic 70.0%
! AACTCCGGTGACGGTGGCGG+TGG + tig0022713:97850-97869 Msa1401690:CDS 70.0%
CCATCCACCGCCACCGTCAC+CGG - tig0022713:97857-97876 None:intergenic 70.0%
! CCGGTGACGGTGGCGGTGGA+TGG + tig0022713:97854-97873 Msa1401690:CDS 75.0%
! CCGGTGACGGTGGCGGTGGA+TGG + tig0022713:97854-97873 Msa1401690:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0022713 gene 97566 99328 97566 ID=Msa1401690;Name=Msa1401690
tig0022713 mRNA 97566 99328 97566 ID=Msa1401690-mRNA-1;Parent=Msa1401690;Name=Msa1401690-mRNA-1;_AED=0.01;_eAED=0.01;_QI=275|1|1|1|1|1|3|387|248
tig0022713 exon 97566 98128 97566 ID=Msa1401690-mRNA-1:exon:18473;Parent=Msa1401690-mRNA-1
tig0022713 exon 98398 98634 98398 ID=Msa1401690-mRNA-1:exon:18474;Parent=Msa1401690-mRNA-1
tig0022713 exon 98720 99328 98720 ID=Msa1401690-mRNA-1:exon:18475;Parent=Msa1401690-mRNA-1
tig0022713 five_prime_UTR 97566 97840 97566 ID=Msa1401690-mRNA-1:five_prime_utr;Parent=Msa1401690-mRNA-1
tig0022713 CDS 97841 98128 97841 ID=Msa1401690-mRNA-1:cds;Parent=Msa1401690-mRNA-1
tig0022713 CDS 98398 98634 98398 ID=Msa1401690-mRNA-1:cds;Parent=Msa1401690-mRNA-1
tig0022713 CDS 98720 98941 98720 ID=Msa1401690-mRNA-1:cds;Parent=Msa1401690-mRNA-1
tig0022713 three_prime_UTR 98942 99328 98942 ID=Msa1401690-mRNA-1:three_prime_utr;Parent=Msa1401690-mRNA-1
Gene Sequence

>Msa1401690

ATGAAGGATAACTCCGGTGACGGTGGCGGTGGATGGAAAAAGGAGGTTGTTTCGGACGTAACTATCACAATTGAAGCTGATGATGATAACAAAGGGGATTATATCAAATTGATGCCTGGTTCCGATGAGTGTTTACCGTTAACTGTGGTGGAGAAAGTGGAGGAGTGTTCTCTGCCGTCAAGACGATCCGTCGTGTGGTATTGGGTCAAAATGGTTCTGTTGTTTCTGAGTTTAGGGTTTTTGGCTGTTGTTGTTCTCAAATGGGTTGGACCCTATCTCATTGACAAGGAGGTGATTCCAATAATAAATTGGGAGACAGAGACTTTCAGTCCTCCGGTGCTTACTATTTTGTTGTTTGCTTCAGTAGCAATTTTCCCAACAATACTTTTGCCATCTACACCCTCTATGTGGGTGGCTGGAGTGACACTTGGTTATGGATTTGGGTTCTTGCTAATAATAACTGCAGCAGCTATTGGTGTGTCACTCCCTTTTATCATTGGCTCAATCTTCCATCATAAAATTGAAGGATGGTTAGAGAAGTATCCAAAGAAGGCTTCTATTCTAAAATCTGCTGGTGCAGGAAACTGGTTTCATCAGTTTAGAGCTGTTGCCTTAATCAGGATTTCTCCGTTCCCTTATATGGTCTTCAATTATTGTGCTGTGGCAACAAATGTTAAGTACGGGCCTTACATAATTGGATCCTTGGTCGGGATGGTGCCAGAAATATTTGTTGCAATCTATACGTAG
Protein sequence

>Msa1401690

MKDNSGDGGGGWKKEVVSDVTITIEADDDNKGDYIKLMPGSDECLPLTVVEKVEECSLPSRRSVVWYWVKMVLLFLSLGFLAVVVLKWVGPYLIDKEVIPIINWETETFSPPVLTILLFASVAIFPTILLPSTPSMWVAGVTLGYGFGFLLIITAAAIGVSLPFIIGSIFHHKIEGWLEKYPKKASILKSAGAGNWFHQFRAVALIRISPFPYMVFNYCAVATNVKYGPYIIGSLVGMVPEIFVAIYT*