AlfalfaGEDB | Msa1299000

M. sativa cultivar zhongmu-4 / Msa1299000

KEGG: -
GO: -
PFAM: PF09335.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299000	G7JK69	95.847	313	8	1	1	308	1	313	0.0	600

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010770	Msa1299000	0.812958	3.262212e-51	-8.615850e-47
Msa0443430	Msa1299000	0.818546	1.852449e-52	-8.615850e-47
Msa0597770	Msa1299000	0.804712	1.893500e-49	-8.615850e-47
Msa0750250	Msa1299000	0.803906	2.787414e-49	-8.615850e-47
Msa0789560	Msa1299000	0.800105	1.684872e-48	-8.615850e-47
Msa1010460	Msa1299000	0.800979	1.117573e-48	-8.615850e-47
Msa1207740	Msa1299000	0.950799	7.785760e-109	-8.615850e-47
Msa1213810	Msa1299000	0.808099	3.657590e-50	-8.615850e-47
Msa1214320	Msa1299000	0.811360	7.280071e-51	-8.615850e-47
Msa1257950	Msa1299000	0.836448	9.301981e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299000	MtrunA17_Chr4g0056711	95.847	313	8	1	1	308	1	313	0.0	600
Msa1299000	MtrunA17_Chr5g0398451	60.084	238	95	0	67	304	77	314	1.55e-111	325
Msa1299000	MtrunA17_Chr2g0291431	42.697	267	150	2	33	299	25	288	7.32e-76	234
Msa1299000	MtrunA17_Chr3g0109441	51.250	80	37	1	187	264	1	80	3.54e-23	90.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299000	AT1G12450.1	59.441	286	113	3	17	302	11	293	7.59e-124	357
Msa1299000	AT4G22850.1	61.566	281	103	2	27	306	19	295	6.84e-118	341
Msa1299000	AT4G12000.1	54.817	301	131	3	13	308	6	306	1.36e-111	326
Msa1299000	AT4G12000.2	54.817	301	131	3	13	308	6	306	1.36e-111	326
Msa1299000	AT4G12000.3	54.817	301	131	3	13	308	6	306	1.36e-111	326
Msa1299000	AT4G22850.2	62.656	241	85	2	27	266	19	255	1.53e-100	296
Msa1299000	AT2G02370.2	40.678	295	158	5	14	302	6	289	1.43e-79	244
Msa1299000	AT2G02370.1	40.678	295	158	5	14	302	6	289	1.43e-79	244
Msa1299000	AT2G02370.3	40.678	295	158	5	14	302	6	289	1.43e-79	244
Msa1299000	AT1G03260.1	26.061	165	116	3	140	302	77	237	5.61e-11	62.4

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Find 120 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCCAGACTAAAACTAC+TGG	0.500282	8_3:+59983407	None:intergenic
AAATGTATTATAACATTGGT+GGG	0.555231	8_3:-59983251	Msa1299000:three_prime_UTR
AACAAAATAGTAAGCACCGG+AGG	0.675222	8_3:+59986265	None:intergenic
AACAACAAAATAGTAAGCAC+CGG	0.597356	8_3:+59986262	None:intergenic
AACTCCGGTGACGGTGGCGG+TGG	0.422523	8_3:-59986855	Msa1299000:CDS
AACTGTGGTGGAGAAAGTGG+AGG	0.671574	8_3:-59986724	Msa1299000:CDS
AAGAAGAGATTAATGAATAA+TGG	0.418233	8_3:+59987018	None:intergenic
AAGATTGAGCCAATGATAAA+AGG	0.256065	8_3:+59986109	None:intergenic
AAGCATTGAGAATGATCTGC+TGG	0.468091	8_3:+59983535	None:intergenic
AAGCTGATGATGATAACAAA+GGG	0.478409	8_3:-59986791	Msa1299000:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATGTTTCGTTTAAGTGAAT+CGG	+	chr8_3:59983182-59983201	None:intergenic	30.0%
GATTATATCAAATTGATGCC+TGG	-	chr8_3:59983373-59983392	Msa1299000:three_prime_UTR	30.0%
TCTGTCTCCCAATTTATTAT+TGG	+	chr8_3:59983846-59983865	None:intergenic	30.0%
AACAACAAAATAGTAAGCAC+CGG	+	chr8_3:59983882-59983901	None:intergenic	30.0%
AGATTGAGCCAATGATAAAA+GGG	+	chr8_3:59984034-59984053	None:intergenic	30.0%
AAGATTGAGCCAATGATAAA+AGG	+	chr8_3:59984035-59984054	None:intergenic	30.0%
ATGTTTCATGAATCTATGCA+GGG	-	chr8_3:59984135-59984154	Msa1299000:intron	30.0%
ACAAAAAATGATACGAGAGT+GGG	+	chr8_3:59984392-59984411	None:intergenic	30.0%
AACAAAAAATGATACGAGAG+TGG	+	chr8_3:59984393-59984412	None:intergenic	30.0%
GCGAATATAGGTTATGTAAT+TGG	-	chr8_3:59984517-59984536	Msa1299000:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	59982992	59987171	59982992	ID=Msa1299000;Name=Msa1299000
chr8_3	mRNA	59982992	59987171	59982992	ID=Msa1299000-mRNA-1;Parent=Msa1299000;Name=Msa1299000-mRNA-1;_AED=0.25;_eAED=0.25;_QI=285\|1\|1\|1\|1\|1\|4\|439\|308
chr8_3	exon	59982992	59983614	59982992	ID=Msa1299000-mRNA-1:exon:17427;Parent=Msa1299000-mRNA-1
chr8_3	exon	59985790	59986007	59985790	ID=Msa1299000-mRNA-1:exon:17426;Parent=Msa1299000-mRNA-1
chr8_3	exon	59986093	59986329	59986093	ID=Msa1299000-mRNA-1:exon:17425;Parent=Msa1299000-mRNA-1
chr8_3	exon	59986599	59987171	59986599	ID=Msa1299000-mRNA-1:exon:17424;Parent=Msa1299000-mRNA-1
chr8_3	five_prime_UTR	59986887	59987171	59986887	ID=Msa1299000-mRNA-1:five_prime_utr;Parent=Msa1299000-mRNA-1
chr8_3	CDS	59986599	59986886	59986599	ID=Msa1299000-mRNA-1:cds;Parent=Msa1299000-mRNA-1
chr8_3	CDS	59986093	59986329	59986093	ID=Msa1299000-mRNA-1:cds;Parent=Msa1299000-mRNA-1
chr8_3	CDS	59985790	59986007	59985790	ID=Msa1299000-mRNA-1:cds;Parent=Msa1299000-mRNA-1
chr8_3	CDS	59983431	59983614	59983431	ID=Msa1299000-mRNA-1:cds;Parent=Msa1299000-mRNA-1
chr8_3	three_prime_UTR	59982992	59983430	59982992	ID=Msa1299000-mRNA-1:three_prime_utr;Parent=Msa1299000-mRNA-1

Gene Sequence

>Msa1299000

ATGAAGGATAACTCCGGTGACGGTGGCGGTGGATGGAAAAAGGAGGTTGTTTCGGACGTAACTATCACAATTGAAGCTGATGATGATAACAAAGGGGATTATATCAAATTGATGCCTGGTTCCGATGAGTGTTTACCGTTAACTGTGGTGGAGAAAGTGGAGGAGTGTTCTCTGCCGTCAAGACGATCCGTCGTGTGGTATTGGGTCAAAATGGTTCTGTTGTTTCTGAGTTTAGGGTTTTTGGCTGTTGTTGTTCTCAAATGGGTTGGACCCTATCTCATTGACAAGGAGGTGATTCCAATAATAAATTGGGAGACAGAGACTTTCAGTCCTCCGGTGCTTACTATTTTGTTGTTTGCTTCAGTAGCAATTTTCCCAACAATACTTTTGCCATCTACACCCTCTATGTGGGTGGCTGGAGTGACACTTGGTTATGGATTTGGGTTCTTGCTAATAATAACTGCAGCAGCTATTGGTGTGTCACTCCCTTTTATCATTGGCTCAATCTTCCATCATAAAATTGAAGGATGGTTAGAGAAGTATCCAAAGAAGGCTTCTATTCTAAAATCTGCTGGTGCAGGAAACTGGTTTCATCAGTTTAGAGCTGTTGCCTTAATCAGGATTTCTCCGTTCCCTTATATGGTCTTCAATTATTGTGCTGTGGCAACAAATGTTAAGTACGGGCCTTACATAATTGGATCCTTGGTCGGGATGGTGCCAGAAATATTTGTTGCAATCTATACGGGAATTTTGATTAAGACATTGGCCGATGCTTCAAGTGAAAGTCAATCTCTATCCGCCCAGCAGATCATTCTCAATGCTTTAGGCTTCTGTTTAACTGTGGTTACAACCGTCATTATCACCGTTTATGCCAAGAGACGGCTGAAGGAGTTGCAGGAGGAGGACAAGATGTTGCTGCTGCAATAG

Protein sequence

>Msa1299000

MKDNSGDGGGGWKKEVVSDVTITIEADDDNKGDYIKLMPGSDECLPLTVVEKVEECSLPSRRSVVWYWVKMVLLFLSLGFLAVVVLKWVGPYLIDKEVIPIINWETETFSPPVLTILLFASVAIFPTILLPSTPSMWVAGVTLGYGFGFLLIITAAAIGVSLPFIIGSIFHHKIEGWLEKYPKKASILKSAGAGNWFHQFRAVALIRISPFPYMVFNYCAVATNVKYGPYIIGSLVGMVPEIFVAIYTGILIKTLADASSESQSLSAQQIILNALGFCLTVVTTVIITVYAKRRLKELQEEDKMLLLQ*