AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1408730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1408730 MtrunA17_Chr4g0044411 98.160 652 12 0 1 652 1 652 0.0 1301
Msa1408730 MtrunA17_Chr4g0031431 44.844 640 293 11 39 651 25 631 3.60e-163 483
Msa1408730 MtrunA17_Chr1g0199321 46.091 614 293 11 46 650 38 622 3.16e-157 466
Msa1408730 MtrunA17_Chr7g0237931 52.255 377 171 3 272 646 236 605 1.77e-123 379
Msa1408730 MtrunA17_Chr7g0248251 37.778 630 338 12 41 652 53 646 2.96e-116 361
Msa1408730 MtrunA17_Chr5g0437411 46.310 393 194 6 273 648 293 685 1.77e-100 322
Msa1408730 MtrunA17_Chr2g0284461 45.876 388 186 10 271 642 290 669 1.41e-86 286
Msa1408730 MtrunA17_Chr7g0234671 37.750 400 233 9 257 649 62 452 2.43e-81 265
Msa1408730 MtrunA17_Chr7g0275081 40.506 395 208 10 267 648 72 452 2.83e-78 256
Msa1408730 MtrunA17_Chr1g0152561 47.826 276 144 0 373 648 61 336 2.22e-72 237
Msa1408730 MtrunA17_Chr7g0261371 48.364 275 141 1 373 647 514 787 5.65e-69 239
Msa1408730 MtrunA17_Chr7g0261371 54.651 86 37 1 263 348 233 316 6.11e-26 113
Msa1408730 MtrunA17_Chr3g0106931 49.823 283 141 1 365 647 545 826 1.74e-68 239
Msa1408730 MtrunA17_Chr3g0106931 51.765 85 41 0 262 346 222 306 2.70e-26 114
Msa1408730 MtrunA17_Chr3g0134761 45.000 280 150 1 373 648 51 330 2.71e-67 223
Msa1408730 MtrunA17_Chr1g0183781 46.182 275 147 1 373 647 440 713 3.24e-66 231
Msa1408730 MtrunA17_Chr1g0183781 31.540 409 235 14 268 646 236 629 9.21e-36 143
Msa1408730 MtrunA17_Chr4g0013091 32.000 650 364 23 39 648 87 698 5.81e-65 228
Msa1408730 MtrunA17_Chr2g0290151 44.444 279 155 0 373 651 175 453 5.84e-63 216
Msa1408730 MtrunA17_Chr4g0059201 28.393 641 403 15 41 651 93 707 2.18e-61 218
Msa1408730 MtrunA17_Chr1g0189811 37.018 389 223 9 273 650 63 440 1.48e-59 206
Msa1408730 MtrunA17_Chr3g0117851 32.061 393 214 11 272 651 274 626 3.98e-48 179
Msa1408730 MtrunA17_Chr3g0122721 29.130 460 249 12 258 651 263 711 6.47e-46 174
Msa1408730 MtrunA17_Chr5g0413681 35.411 401 222 13 275 651 282 669 1.45e-43 166
Msa1408730 MtrunA17_Chr2g0280671 30.357 392 233 9 275 651 17 383 7.09e-36 139
Msa1408730 MtrunA17_Chr8g0377151 27.397 438 245 9 274 648 283 710 1.17e-35 143
Msa1408730 MtrunA17_Chr8g0377151 23.420 269 155 6 392 610 447 714 1.13e-11 68.2
Msa1408730 MtrunA17_Chr3g0118001 32.491 277 182 2 374 647 212 486 2.71e-35 140
Msa1408730 MtrunA17_Chr4g0044631 30.964 394 242 12 270 647 11 390 6.36e-34 134
Msa1408730 MtrunA17_Chr3g0110121 26.000 400 261 11 269 646 32 418 7.46e-32 129
Msa1408730 MtrunA17_Chr4g0048811 29.268 328 200 8 273 572 5 328 1.53e-29 119
Msa1408730 MtrunA17_Chr1g0198984 49.123 114 56 1 286 399 1 112 2.26e-28 109
Msa1408730 MtrunA17_Chr5g0429231 32.517 286 176 5 373 647 34 313 1.26e-26 111
Msa1408730 MtrunA17_Chr5g0420471 26.961 408 248 16 271 644 23 414 2.12e-26 112
Msa1408730 MtrunA17_Chr3g0137061 30.721 319 205 7 342 647 79 394 2.80e-26 113
Msa1408730 MtrunA17_Chr5g0434171 24.495 396 269 11 271 646 33 418 4.12e-26 111
Msa1408730 MtrunA17_Chr6g0479261 26.238 404 256 14 271 644 23 414 4.78e-26 111
Msa1408730 MtrunA17_Chr6g0451581 27.481 262 182 3 385 643 253 509 2.38e-25 110
Msa1408730 MtrunA17_Chr5g0400971 25.429 350 238 8 273 616 67 399 3.13e-25 109
Msa1408730 MtrunA17_Chr8g0373031 51.282 78 38 0 271 348 273 350 2.11e-24 108
Msa1408730 MtrunA17_Chr8g0384041 26.829 369 215 14 270 605 7 353 2.80e-24 105
Msa1408730 MtrunA17_Chr5g0404311 48.864 88 45 0 261 348 261 348 3.34e-24 108
Msa1408730 MtrunA17_Chr7g0267501 28.013 307 185 10 268 550 3 297 4.30e-24 105
Msa1408730 MtrunA17_Chr1g0177261 27.302 315 171 9 277 547 11 311 2.31e-23 103
Msa1408730 MtrunA17_Chr1g0149941 31.365 271 172 7 389 647 190 458 4.51e-23 103
Msa1408730 MtrunA17_Chr1g0177281 27.619 315 170 9 277 547 11 311 5.30e-23 102
Msa1408730 MtrunA17_Chr3g0129811 26.389 360 224 13 271 605 81 424 5.39e-23 102
Msa1408730 MtrunA17_Chr1g0152291 28.818 347 215 10 273 606 45 372 1.06e-22 101
Msa1408730 MtrunA17_Chr1g0199031 46.552 116 45 1 450 565 18 116 1.18e-22 94.0
Msa1408730 MtrunA17_Chr1g0177271 25.359 418 247 14 277 644 11 413 6.86e-22 99.0
Msa1408730 MtrunA17_Chr2g0278251 45.763 118 60 3 420 535 5 120 1.07e-20 89.4
Msa1408730 MtrunA17_Chr8g0353271 29.167 288 186 8 373 643 8 294 2.10e-19 90.1
Msa1408730 MtrunA17_Chr2g0322021 29.642 307 184 10 269 559 2 292 3.49e-19 90.5
Msa1408730 MtrunA17_Chr3g0144071 25.294 340 186 8 273 545 30 368 5.05e-18 88.6
Msa1408730 MtrunA17_Chr1g0177301 26.273 373 219 13 269 588 2 371 1.28e-17 85.5
Msa1408730 MtrunA17_Chr1g0196911 28.571 231 132 6 233 440 462 682 7.19e-16 82.0
Msa1408730 MtrunA17_Chr8g0390311 51.389 72 33 2 274 344 396 466 1.34e-15 81.3
Msa1408730 MtrunA17_Chr5g0435041 28.514 249 129 6 405 647 92 297 2.75e-15 78.2
Msa1408730 MtrunA17_Chr5g0412171 44.776 67 37 0 273 339 732 798 9.90e-15 77.8
Msa1408730 MtrunA17_Chr8g0346441 24.793 363 229 9 275 604 261 612 3.46e-14 76.3
Msa1408730 MtrunA17_Chr7g0275961 25.628 398 239 11 275 644 257 625 4.79e-14 75.9
Msa1408730 MtrunA17_Chr1g0202971 45.455 66 36 0 274 339 649 714 1.04e-13 74.7
Msa1408730 MtrunA17_Chr7g0247831 44.286 70 39 0 274 343 827 896 5.51e-13 72.4
Msa1408730 MtrunA17_Chr4g0025501 30.065 153 91 6 271 419 4 144 5.79e-13 71.2
Msa1408730 MtrunA17_Chr2g0328101 46.269 67 36 0 273 339 740 806 6.55e-13 72.0
Msa1408730 MtrunA17_Chr3g0111451 30.576 278 154 7 372 648 80 319 1.70e-12 69.7
Msa1408730 MtrunA17_Chr1g0199021 82.353 34 6 0 272 305 24 57 3.64e-12 61.6
Msa1408730 MtrunA17_Chr7g0259851 28.571 217 151 3 371 584 183 398 5.74e-12 68.9
Msa1408730 MtrunA17_Chr6g0449461 27.273 275 160 5 376 647 44 281 8.18e-12 67.0
Msa1408730 MtrunA17_Chr4g0070941 41.667 72 41 1 268 339 686 756 1.06e-11 68.2
Msa1408730 MtrunA17_Chr5g0394261 28.500 200 141 2 371 568 193 392 1.17e-11 68.2
Msa1408730 MtrunA17_Chr7g0215741 27.749 191 134 2 366 552 551 741 1.94e-11 67.4
Msa1408730 MtrunA17_Chr8g0367341 37.374 99 59 2 243 339 695 792 2.15e-11 67.4
Msa1408730 MtrunA17_Chr1g0188041 22.649 521 341 20 117 594 99 600 5.08e-11 66.2
Msa1408730 MtrunA17_Chr1g0205751 27.500 200 143 2 371 568 196 395 7.42e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1408730 AT1G71020.1 61.751 651 215 10 1 648 1 620 0.0 729
Msa1408730 AT1G23030.1 60.465 645 206 9 13 651 11 612 0.0 716
Msa1408730 AT1G71020.2 65.638 486 149 6 165 648 3 472 0.0 595
Msa1408730 AT3G46510.1 44.844 640 289 11 40 651 29 632 2.78e-162 481
Msa1408730 AT3G54850.1 43.902 615 306 9 46 650 39 624 5.75e-148 443
Msa1408730 AT2G28830.1 45.604 546 277 10 118 649 24 563 4.43e-141 424
Msa1408730 AT5G42340.1 37.361 629 334 10 41 652 75 660 1.38e-112 353
Msa1408730 AT1G29340.1 36.709 553 303 15 123 642 143 681 1.75e-80 270
Msa1408730 AT3G07360.1 37.755 392 231 8 269 652 70 456 2.43e-76 251
Msa1408730 AT2G23140.3 48.410 283 141 2 369 647 346 627 3.44e-71 243
Msa1408730 AT2G23140.3 51.899 79 38 0 270 348 38 116 3.05e-25 111
Msa1408730 AT2G23140.5 48.410 283 141 2 369 647 346 627 3.44e-71 243
Msa1408730 AT2G23140.5 51.899 79 38 0 270 348 38 116 3.05e-25 111
Msa1408730 AT2G23140.2 47.405 289 151 1 359 647 529 816 3.82e-70 244
Msa1408730 AT2G23140.2 51.899 79 38 0 270 348 227 305 5.17e-25 111
Msa1408730 AT2G23140.4 47.405 289 151 1 359 647 529 816 3.82e-70 244
Msa1408730 AT2G23140.4 51.899 79 38 0 270 348 227 305 5.17e-25 111
Msa1408730 AT2G23140.1 47.405 289 151 1 359 647 532 819 4.83e-70 243
Msa1408730 AT2G23140.1 51.899 79 38 0 270 348 230 308 5.57e-25 111
Msa1408730 AT5G67340.1 34.172 477 225 9 261 651 228 701 5.17e-70 241
Msa1408730 AT5G67340.2 34.172 477 225 9 261 651 228 701 5.17e-70 241
Msa1408730 AT3G01400.1 45.848 277 149 1 373 648 64 340 1.10e-67 225
Msa1408730 AT5G01830.1 30.781 640 366 21 39 648 53 645 7.98e-65 226
Msa1408730 AT5G58680.1 44.245 278 153 2 373 648 62 339 3.55e-64 216
Msa1408730 AT5G18320.1 36.160 401 209 13 273 649 72 449 5.02e-59 205
Msa1408730 AT3G54790.2 46.718 259 136 2 390 647 455 712 5.27e-59 211
Msa1408730 AT3G54790.2 50.000 70 35 0 270 339 199 268 5.70e-19 92.0
Msa1408730 AT3G54790.3 46.718 259 136 2 390 647 491 748 9.63e-59 211
Msa1408730 AT3G54790.3 50.000 70 35 0 270 339 235 304 8.32e-19 91.3
Msa1408730 AT3G54790.1 46.718 259 136 2 390 647 491 748 9.63e-59 211
Msa1408730 AT3G54790.1 50.000 70 35 0 270 339 235 304 8.32e-19 91.3
Msa1408730 AT4G16490.1 45.038 262 142 1 389 648 201 462 2.68e-56 198
Msa1408730 AT1G27910.1 29.861 432 249 9 272 651 278 707 1.22e-46 177
Msa1408730 AT1G24330.1 28.425 489 272 12 222 648 234 706 4.38e-46 175
Msa1408730 AT1G24330.2 28.425 489 272 12 222 648 234 706 4.38e-46 175
Msa1408730 AT1G67530.2 29.825 456 246 13 259 651 266 710 4.71e-44 169
Msa1408730 AT1G67530.1 29.825 456 246 13 259 651 266 710 4.71e-44 169
Msa1408730 AT1G67530.4 29.825 456 246 13 259 651 266 710 4.71e-44 169
Msa1408730 AT3G07360.5 34.049 326 202 8 335 652 1 321 1.50e-43 159
Msa1408730 AT3G07360.3 34.049 326 202 8 335 652 1 321 1.50e-43 159
Msa1408730 AT3G07360.9 34.049 326 202 8 335 652 1 321 1.50e-43 159
Msa1408730 AT3G07360.6 34.049 326 202 8 335 652 1 321 1.50e-43 159
Msa1408730 AT3G07360.2 34.049 326 202 8 335 652 1 321 1.50e-43 159
Msa1408730 AT3G07360.8 34.049 326 202 8 335 652 1 321 1.50e-43 159
Msa1408730 AT3G07360.10 34.049 326 202 8 335 652 1 321 1.50e-43 159
Msa1408730 AT3G07360.7 34.049 326 202 8 335 652 1 321 1.50e-43 159
Msa1408730 AT3G07360.4 34.049 326 202 8 335 652 1 321 1.50e-43 159
Msa1408730 AT4G21350.1 31.043 393 228 7 270 646 2 367 3.30e-41 154
Msa1408730 AT5G18330.1 31.884 345 208 12 273 606 65 393 2.51e-39 150
Msa1408730 AT1G60190.1 30.151 398 247 12 275 651 280 667 1.29e-37 149
Msa1408730 AT3G19380.1 32.184 348 218 8 271 605 12 354 3.61e-37 144
Msa1408730 AT1G49780.1 31.899 395 244 9 271 647 12 399 4.87e-36 140
Msa1408730 AT1G10560.1 25.424 649 410 20 48 651 59 678 9.85e-36 144
Msa1408730 AT5G18340.1 30.000 380 228 15 246 601 41 406 2.58e-35 139
Msa1408730 AT5G62560.1 32.258 279 182 3 374 647 237 513 1.94e-33 135
Msa1408730 AT5G40140.1 30.576 278 187 3 374 647 231 506 4.45e-31 128
Msa1408730 AT5G65200.1 30.769 286 185 4 374 648 227 510 6.17e-31 128
Msa1408730 AT5G37490.1 24.292 424 290 11 248 652 6 417 1.91e-27 116
Msa1408730 AT2G35930.1 29.592 294 179 10 269 549 8 286 3.81e-26 112
Msa1408730 AT4G31890.3 34.545 275 164 8 387 647 156 428 4.02e-26 113
Msa1408730 AT4G31890.2 34.545 275 164 8 387 647 156 428 4.02e-26 113
Msa1408730 AT4G31890.1 34.545 275 164 8 387 647 156 428 4.02e-26 113
Msa1408730 AT5G65920.1 26.357 387 246 12 273 644 60 422 2.76e-25 109
Msa1408730 AT5G65920.2 26.357 387 246 12 273 644 60 422 2.76e-25 109
Msa1408730 AT3G47820.1 28.975 283 189 6 374 647 208 487 3.07e-24 107
Msa1408730 AT1G66160.1 26.650 409 244 16 271 652 31 410 3.48e-24 106
Msa1408730 AT3G49810.1 25.316 395 256 10 265 644 56 426 4.25e-24 106
Msa1408730 AT1G67530.3 48.913 92 39 1 259 350 266 349 8.36e-24 106
Msa1408730 AT4G12710.1 31.835 267 173 5 391 648 65 331 6.42e-23 102
Msa1408730 AT1G08315.1 34.146 246 149 8 373 606 9 253 1.29e-22 99.8
Msa1408730 AT5G09800.1 26.211 351 224 12 273 605 11 344 1.30e-21 98.2
Msa1408730 AT5G64660.1 26.837 313 189 11 270 579 7 282 5.14e-21 96.7
Msa1408730 AT3G52450.1 26.705 352 193 13 271 574 5 339 2.70e-20 94.7
Msa1408730 AT2G25130.1 30.855 269 168 8 393 647 131 395 7.95e-20 93.6
Msa1408730 AT3G18710.1 27.160 324 197 14 271 573 10 315 3.86e-18 87.8
Msa1408730 AT3G03440.1 31.071 280 181 6 378 647 68 345 7.87e-18 86.7
Msa1408730 AT4G36550.1 43.243 74 42 0 273 346 219 292 1.05e-16 84.7
Msa1408730 AT1G66160.2 24.694 409 236 17 271 652 31 394 4.97e-15 78.2
Msa1408730 AT3G11840.1 44.304 79 42 2 266 343 3 80 5.19e-15 78.6
Msa1408730 AT1G76390.1 25.816 337 182 13 273 542 25 360 6.35e-15 79.0
Msa1408730 AT1G76390.2 25.816 337 182 13 273 542 25 360 6.35e-15 79.0
Msa1408730 AT1G01680.1 46.154 65 35 0 275 339 235 299 9.80e-15 75.9
Msa1408730 AT2G45920.1 46.970 66 35 0 274 339 326 391 1.23e-14 76.6
Msa1408730 AT5G50900.1 29.048 210 146 2 376 582 149 358 3.37e-14 76.3
Msa1408730 AT1G01660.1 45.455 66 36 0 274 339 498 563 4.50e-14 75.9
Msa1408730 AT1G01660.2 45.455 66 36 0 274 339 499 564 4.59e-14 75.9
Msa1408730 AT1G56040.1 47.692 65 34 0 274 338 354 418 9.88e-14 74.3
Msa1408730 AT3G61390.4 43.939 66 37 0 274 339 279 344 1.06e-13 73.6
Msa1408730 AT3G61390.2 43.939 66 37 0 274 339 354 419 1.44e-13 73.9
Msa1408730 AT5G65500.1 46.269 67 36 0 273 339 715 781 1.63e-13 74.3
Msa1408730 AT5G65500.2 46.269 67 36 0 273 339 723 789 1.73e-13 74.3
Msa1408730 AT5G57035.1 46.970 66 35 0 274 339 709 774 3.25e-13 73.6
Msa1408730 AT3G20170.1 29.885 174 117 1 409 582 271 439 5.45e-13 72.0
Msa1408730 AT1G01670.3 40.541 74 44 0 270 343 207 280 6.55e-13 70.1
Msa1408730 AT1G01670.2 40.541 74 44 0 270 343 291 364 1.08e-12 70.5
Msa1408730 AT1G01670.1 40.541 74 44 0 270 343 291 364 1.08e-12 70.5
Msa1408730 AT2G45910.2 35.632 87 56 0 257 343 747 833 2.12e-12 70.9
Msa1408730 AT2G45910.1 35.632 87 56 0 257 343 747 833 2.12e-12 70.9
Msa1408730 AT5G13060.1 30.415 217 147 4 371 584 225 440 2.37e-12 70.9
Msa1408730 AT1G20780.1 24.397 373 224 13 273 601 23 381 3.92e-12 70.1
Msa1408730 AT1G20780.2 24.397 373 224 13 273 601 23 381 3.92e-12 70.1
Msa1408730 AT1G20780.3 24.397 373 224 13 273 601 23 381 3.92e-12 70.1
Msa1408730 AT1G56030.1 41.538 65 38 0 274 338 324 388 6.28e-12 68.6
Msa1408730 AT2G44900.1 27.513 189 133 2 368 552 564 752 1.08e-11 68.9
Msa1408730 AT2G44900.2 27.513 189 133 2 368 552 564 752 1.08e-11 68.9
Msa1408730 AT2G19410.3 45.455 66 35 1 274 339 712 776 1.18e-11 68.6
Msa1408730 AT2G19410.2 45.455 66 35 1 274 339 747 811 1.19e-11 68.6
Msa1408730 AT2G19410.1 45.455 66 35 1 274 339 726 790 1.24e-11 68.6
Msa1408730 AT1G68940.3 25.387 323 202 11 277 588 250 544 6.94e-11 66.2
Msa1408730 AT1G68940.2 25.387 323 202 11 277 588 303 597 9.95e-11 65.9

Find 170 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAAATTTCTTGACTTTC+AGG 0.038417 tig0023410:-71392 None:intergenic
AGATAAATATATTAACTTTA+AGG 0.166456 tig0023410:-72722 None:intergenic
TCCCTCTAAGACTTCCATTT+TGG 0.189510 tig0023410:-72253 None:intergenic
CCTGAAAGTCAAGAAATTTC+TGG 0.194581 tig0023410:+71392 Msa1408730:CDS
ACGGAAGGCTACTTCATTAT+TGG 0.201892 tig0023410:+72649 Msa1408730:CDS
CCTATTGTCAGTGCTTCTTT+TGG 0.240978 tig0023410:-71937 None:intergenic
AGACTCCTTAGAACATAATT+TGG 0.262335 tig0023410:-71723 None:intergenic
TTACATCTTCTGAAGTTAAA+AGG 0.270561 tig0023410:-72001 None:intergenic
TCAGCGCGTGACGTGGAAAT+TGG 0.296094 tig0023410:+70900 Msa1408730:CDS
TGTGATGATCCCTGCCCTTA+TGG 0.298109 tig0023410:-72336 None:intergenic
TGATTTCTTGAGCCAGGGGC+TGG 0.320586 tig0023410:-71178 None:intergenic
AAACTGAAAGATAATCTTCT+TGG 0.322536 tig0023410:-70878 None:intergenic
TCACCGGCCATTGTATTTGC+TGG 0.324513 tig0023410:-70141 None:intergenic
GTTAAAAGGTTGACTAAAAC+TGG 0.330673 tig0023410:-71987 None:intergenic
TTACACCAAATTATGTTCTA+AGG 0.332391 tig0023410:+71718 Msa1408730:CDS
CAGTTTACCGTATGACGATT+TGG 0.342582 tig0023410:+70936 Msa1408730:CDS
CTCATTAGCTCCAACGATAT+AGG 0.343106 tig0023410:-71479 None:intergenic
TGGTGCCGTCATACCTCTTT+CGG 0.345816 tig0023410:+72595 Msa1408730:CDS
AGAGATTACGGAGCTGAATA+AGG 0.352743 tig0023410:+70329 Msa1408730:CDS
CTCAAAAGATCTATTAAAAC+TGG 0.356638 tig0023410:-72479 None:intergenic
TCCTCCTCCTGCGCACATTC+CGG 0.357761 tig0023410:-70214 None:intergenic
TTAGAACATAATTTGGTGTA+AGG 0.358392 tig0023410:-71716 None:intergenic
CTCCAAAATGGAAGTCTTAG+AGG 0.362705 tig0023410:+72251 Msa1408730:CDS
AAGGGACTTATTATGCTTGC+TGG 0.364775 tig0023410:+72080 Msa1408730:CDS
TCATTAGCTCCAACGATATA+GGG 0.368285 tig0023410:-71478 None:intergenic
TGGAAACCTTTGCCTCTTGA+TGG 0.369775 tig0023410:-72436 None:intergenic
TCATTTGTTCGAAGAGATTA+CGG 0.373183 tig0023410:+70317 Msa1408730:CDS
CGATTCTGCTTCGAGTTCTT+CGG 0.376648 tig0023410:+70356 Msa1408730:CDS
TTCAATTTATGTATATATCA+AGG 0.380676 tig0023410:+72299 Msa1408730:CDS
TTGGTAGATTTGCTCCAAAA+TGG 0.381246 tig0023410:+72239 Msa1408730:CDS
GAAAGAAGAAAAGAATTAAC+TGG 0.381898 tig0023410:-72832 None:intergenic
CTTGCACAAAGCAAGTAAAA+TGG 0.385872 tig0023410:-72537 None:intergenic
TGACCAGTGGCCACAATAAC+GGG 0.386961 tig0023410:-71506 None:intergenic
CAAGAAATCAGTCAGGTTCT+TGG 0.388483 tig0023410:+71191 Msa1408730:CDS
TATCCGTTGCTCGTCTCAAT+TGG 0.397671 tig0023410:-71112 None:intergenic
CGTTATTGTGGCCACTGGTC+AGG 0.400672 tig0023410:+71508 Msa1408730:CDS
CGTCATACCTCTTTCGGAAC+TGG 0.401096 tig0023410:+72601 Msa1408730:CDS
CCAAAAGAAGCACTGACAAT+AGG 0.425006 tig0023410:+71937 Msa1408730:CDS
GATTTCTTGAGCCAGGGGCT+GGG 0.426469 tig0023410:-71177 None:intergenic
CTGAAAGTCAAGAAATTTCT+GGG 0.428562 tig0023410:+71393 Msa1408730:CDS
TGCTTCGAGTTCTTCGGTTA+CGG 0.434500 tig0023410:+70362 Msa1408730:CDS
CTCAGGAGCTATATCTGCTT+TGG 0.437435 tig0023410:+72220 Msa1408730:CDS
GAACAAGGGCAGAGCCATAA+GGG 0.442885 tig0023410:+72322 Msa1408730:CDS
GATGAGAACAAAATAATCAT+TGG 0.443072 tig0023410:+72194 Msa1408730:CDS
AAAATAATCATTGGAGCCTC+AGG 0.443695 tig0023410:+72203 Msa1408730:CDS
AACCTTTGCCTCTTGATGGC+TGG 0.444911 tig0023410:-72432 None:intergenic
CCTGACTGATTTCTTGAGCC+AGG 0.449242 tig0023410:-71184 None:intergenic
CGCTGATACAGAGGTTGAAA+AGG 0.449352 tig0023410:-72866 None:intergenic
ATAGGGCAAAGAAAATCTTC+AGG 0.449853 tig0023410:-71461 None:intergenic
CTCTCCTGTATCAGTAGACT+TGG 0.451352 tig0023410:+72575 Msa1408730:CDS
TGGAAGTCTTAGAGGGAAGA+AGG 0.455734 tig0023410:+72259 Msa1408730:CDS
CTGACCAGTGGCCACAATAA+CGG 0.457070 tig0023410:-71507 None:intergenic
CAAGAACTGACATTATTGTA+AGG 0.458400 tig0023410:-72409 None:intergenic
AACCGAGATCCTGCTGCAAA+CGG 0.459758 tig0023410:-71311 None:intergenic
CTGACCTGCAGGAAAGCTTC+CGG 0.460846 tig0023410:-71872 None:intergenic
TTTCCGTTGGTTAGTCCTGT+AGG 0.462136 tig0023410:-71783 None:intergenic
GAACATTGCATCGGCGGTGA+TGG 0.464055 tig0023410:-70250 None:intergenic
CAAACTGATCAGATCAATTG+AGG 0.464066 tig0023410:+72781 Msa1408730:three_prime_UTR
AGGATACTAATTGCTGAAGC+TGG 0.467489 tig0023410:+71957 Msa1408730:CDS
GGATCGTTCAGAGATGTTAC+AGG 0.468224 tig0023410:+71822 Msa1408730:CDS
GAGCTCCGGCGTGTTGTCTC+CGG 0.469715 tig0023410:-70169 None:intergenic
CTTTCGGAACTGGCTAGAAC+TGG 0.469776 tig0023410:+72611 Msa1408730:CDS
CTCGGAAGAAGTCAAAGAAC+AGG 0.471166 tig0023410:+70963 Msa1408730:CDS
CTATATCGTTGGAGCTAATG+AGG 0.476241 tig0023410:+71480 Msa1408730:CDS
ACCGGAATGTGCGCAGGAGG+AGG 0.478616 tig0023410:+70213 Msa1408730:CDS
TGAATTAAGCACAGGAGCTC+CGG 0.479194 tig0023410:-70183 None:intergenic
GGAACAAGGGCAGAGCCATA+AGG 0.479718 tig0023410:+72321 Msa1408730:CDS
AGCTGCAACCCGTTTGCAGC+AGG 0.482955 tig0023410:+71302 Msa1408730:CDS
ACCCGTTTGCAGCAGGATCT+CGG 0.482980 tig0023410:+71309 Msa1408730:CDS
AGCATTCCTAAAAGCAATGA+AGG 0.486039 tig0023410:+71275 Msa1408730:CDS
AGAGATCCGGCGAACTAGAT+CGG 0.487487 tig0023410:-70286 None:intergenic
ACCGAGATCCTGCTGCAAAC+GGG 0.489778 tig0023410:-71310 None:intergenic
AGCTCGGAGGACTTGAACAA+TGG 0.490269 tig0023410:-72114 None:intergenic
CCTGAAAATCTATCGGAACT+AGG 0.494469 tig0023410:+71251 Msa1408730:CDS
GTCAAGAAATTTCTGGGAGT+GGG 0.498309 tig0023410:+71399 Msa1408730:CDS
ACACTCCTCAACTGACCTGC+AGG 0.499381 tig0023410:-71883 None:intergenic
AGTCAAGAAATTTCTGGGAG+TGG 0.500581 tig0023410:+71398 Msa1408730:CDS
TAATGAAGTAGCCTTCCGTT+TGG 0.503664 tig0023410:-72645 None:intergenic
GGCACCAAGTCTACTGATAC+AGG 0.505124 tig0023410:-72579 None:intergenic
TTTCGAATTCAGAGGATTCG+TGG 0.507183 tig0023410:+70385 Msa1408730:CDS
AGGTTGAAAAGGAACAATAC+AGG 0.508421 tig0023410:-72855 None:intergenic
CACAGATTCAAGCAAATCAA+TGG 0.510591 tig0023410:+72376 Msa1408730:CDS
AAGGGCAGAGCCATAAGGGC+AGG 0.511128 tig0023410:+72326 Msa1408730:CDS
AGACGAGCAACGGATAAATA+CGG 0.513807 tig0023410:+71119 Msa1408730:CDS
ATTGTTCAAGTCCTCCGAGC+TGG 0.515684 tig0023410:+72116 Msa1408730:CDS
GGAATGTGCGCAGGAGGAGG+AGG 0.517669 tig0023410:+70216 Msa1408730:CDS
TATTAAAACTGGTATAGTGC+TGG 0.519102 tig0023410:-72468 None:intergenic
TAGAACATAATTTGGTGTAA+GGG 0.520468 tig0023410:-71715 None:intergenic
TTCAGTTTCAGCGCGTGACG+TGG 0.525877 tig0023410:+70893 Msa1408730:CDS
TGCCAGCCATCAAGAGGCAA+AGG 0.525971 tig0023410:+72430 Msa1408730:CDS
GTTCTAGCCAGTTCCGAAAG+AGG 0.528227 tig0023410:-72608 None:intergenic
TTGGTGTAAGGGTCAAATGT+TGG 0.530471 tig0023410:-71704 None:intergenic
TCAATTTATGTATATATCAA+GGG 0.531108 tig0023410:+72300 Msa1408730:CDS
CGGAGGACTTGAACAATGGA+TGG 0.531661 tig0023410:-72110 None:intergenic
CGCCGATGCAATGTTCAGAA+AGG 0.532617 tig0023410:+70257 Msa1408730:CDS
TTGCTTCCATGGTTCCAGCT+CGG 0.533674 tig0023410:-72130 None:intergenic
CTGACTGATTTCTTGAGCCA+GGG 0.534115 tig0023410:-71183 None:intergenic
CACAACATCGAGCAACCTAC+AGG 0.539474 tig0023410:+71768 Msa1408730:CDS
TGTTCTCATCCGCAAGTGAA+AGG 0.539476 tig0023410:-72181 None:intergenic
CTTTCTGAACATTGCATCGG+CGG 0.540344 tig0023410:-70256 None:intergenic
CAGCAATTGAAACTCTCGTC+CGG 0.543348 tig0023410:+71853 Msa1408730:CDS
ATCCTTTCTGAACATTGCAT+CGG 0.545375 tig0023410:-70259 None:intergenic
TGCTTAATTCACGACATCAC+CGG 0.546924 tig0023410:+70195 Msa1408730:CDS
CCATTGTATTTGCTGGATTG+TGG 0.547440 tig0023410:-70134 None:intergenic
GTTGTCTCCGGCGAACTCAC+CGG 0.547717 tig0023410:-70157 None:intergenic
AACTTTACACGCTGATACAG+AGG 0.552438 tig0023410:-72875 None:intergenic
CATTGCATCGGCGGTGATGG+AGG 0.554742 tig0023410:-70247 None:intergenic
TCGTCCGGAAGCTTTCCTGC+AGG 0.555944 tig0023410:+71868 Msa1408730:CDS
GCTGCGTTCTCTTTGTTGCG+AGG 0.556404 tig0023410:-72512 None:intergenic
AGTCCTCCGAGCTGGAACCA+TGG 0.557782 tig0023410:+72124 Msa1408730:CDS
GCTTTCCTGCAGGTCAGTTG+AGG 0.557800 tig0023410:+71878 Msa1408730:CDS
CGATAAGGAAACCTCGGAAG+AGG 0.558738 tig0023410:-71357 None:intergenic
AATGAGTAAGAAGAGAGATC+CGG 0.559409 tig0023410:-70299 None:intergenic
GTTTGCAGCAGGATCTCGGT+TGG 0.561113 tig0023410:+71313 Msa1408730:CDS
ATGTATATATCAAGGGAACA+AGG 0.561345 tig0023410:+72307 Msa1408730:CDS
TAGCTGTCCTGAAAATCTAT+CGG 0.562858 tig0023410:+71244 Msa1408730:CDS
AACAAGACTAACAAATCAAG+AGG 0.564368 tig0023410:-72951 None:intergenic
AATCCAGCAAATACAATGGC+CGG 0.565831 tig0023410:+70138 Msa1408730:exon
GACGAGCAACGGATAAATAC+GGG 0.566709 tig0023410:+71120 Msa1408730:CDS
ATCGATCCATCTCTGTATGT+AGG 0.566858 tig0023410:-71643 None:intergenic
AAGAACTGACATTATTGTAA+GGG 0.567464 tig0023410:-72408 None:intergenic
GACATCACCGGAATGTGCGC+AGG 0.567727 tig0023410:+70207 Msa1408730:CDS
TTTACTTGCTTTGTGCAAGA+GGG 0.567749 tig0023410:+72541 Msa1408730:CDS
AATGAGGGATCCCGTTATTG+TGG 0.571734 tig0023410:+71496 Msa1408730:CDS
AGGAAACCTCGGAAGAGGCA+TGG 0.571746 tig0023410:-71352 None:intergenic
ATACAGAGATGGATCGATTG+TGG 0.572889 tig0023410:+71648 Msa1408730:CDS
ATTAACTGGATCAATGAGGC+TGG 0.574702 tig0023410:-72818 None:intergenic
TAAGGAGTCTTGTTTCTCAG+TGG 0.575605 tig0023410:+71736 Msa1408730:CDS
CCTGGCTCAAGAAATCAGTC+AGG 0.580247 tig0023410:+71184 Msa1408730:CDS
GTATGACGATTTGGATATCT+CGG 0.583393 tig0023410:+70945 Msa1408730:CDS
CTGGTACAGAGCGAGCCAAA+CGG 0.590641 tig0023410:+72630 Msa1408730:CDS
ATCACCGGAATGTGCGCAGG+AGG 0.591840 tig0023410:+70210 Msa1408730:CDS
TTTAATCGATAAGGAAACCT+CGG 0.595093 tig0023410:-71363 None:intergenic
TGATGTCGTGAATTAAGCAC+AGG 0.596241 tig0023410:-70191 None:intergenic
GATCCCGTTATTGTGGCCAC+TGG 0.597895 tig0023410:+71503 Msa1408730:CDS
TTCTATATATGAAAACAACA+AGG 0.600201 tig0023410:+72061 Msa1408730:CDS
AGTTCTTGCCAGCCATCAAG+AGG 0.603879 tig0023410:+72424 Msa1408730:CDS
CAAAGCAGATATAGCTCCTG+AGG 0.605504 tig0023410:-72219 None:intergenic
TGACTGATTTCTTGAGCCAG+GGG 0.608554 tig0023410:-71182 None:intergenic
TACAACAGCTTTGAAATGCG+AGG 0.610979 tig0023410:+72687 Msa1408730:exon
AGCGATCCTACATACAGAGA+TGG 0.611283 tig0023410:+71637 Msa1408730:CDS
CAGTTCCGAAAGAGGTATGA+CGG 0.611470 tig0023410:-72600 None:intergenic
TCCAAAATGGAAGTCTTAGA+GGG 0.612631 tig0023410:+72252 Msa1408730:CDS
GAGTATCCATGCCTCTTCCG+AGG 0.613423 tig0023410:+71346 Msa1408730:CDS
GTTCTTGGCAAATCTGTGAG+TGG 0.616724 tig0023410:+71206 Msa1408730:CDS
GAGATATCCAAATCGTCATA+CGG 0.619518 tig0023410:-70943 None:intergenic
GGTTGAAAAGGAACAATACA+GGG 0.620210 tig0023410:-72854 None:intergenic
AAGAGCAAGCACTAGATCAG+AGG 0.622188 tig0023410:-70409 None:intergenic
GGCTGGGATGAATCAAAAGA+CGG 0.622684 tig0023410:-71161 None:intergenic
GGTTACGGTTTCGAATTCAG+AGG 0.625095 tig0023410:+70377 Msa1408730:CDS
CTTCCATGGTTCCAGCTCGG+AGG 0.625169 tig0023410:-72127 None:intergenic
ATTGCACCGATCTAGTTCGC+CGG 0.632440 tig0023410:+70280 Msa1408730:CDS
CATGGAAGCAAGAGAAAACG+CGG 0.632928 tig0023410:+72142 Msa1408730:CDS
AGGGCAGAGCCATAAGGGCA+GGG 0.634588 tig0023410:+72327 Msa1408730:CDS
TATATCGTTGGAGCTAATGA+GGG 0.638648 tig0023410:+71481 Msa1408730:CDS
AAGTCAGAGAGAGTCTAATG+AGG 0.639756 tig0023410:-70109 None:intergenic
AAACCAATTGAGACGAGCAA+CGG 0.643111 tig0023410:+71109 Msa1408730:CDS
AAGAATTAACTGGATCAATG+AGG 0.643213 tig0023410:-72822 None:intergenic
TCTATATATGAAAACAACAA+GGG 0.645718 tig0023410:+72062 Msa1408730:CDS
TGTAATGACGCAAGAAAACG+CGG 0.646941 tig0023410:+72019 Msa1408730:CDS
GCATTCCTAAAAGCAATGAA+GGG 0.647570 tig0023410:+71276 Msa1408730:CDS
TGTATATATCAAGGGAACAA+GGG 0.653092 tig0023410:+72308 Msa1408730:CDS
ACAATGGCCGGTGAGTTCGC+CGG 0.654301 tig0023410:+70150 Msa1408730:exon
TACAGAGCGAGCCAAACGGA+AGG 0.654338 tig0023410:+72634 Msa1408730:CDS
AGATTCTCAAGCATATAGCA+TGG 0.658577 tig0023410:+72995 Msa1408730:three_prime_UTR
CCACAATCCAGCAAATACAA+TGG 0.669742 tig0023410:+70134 Msa1408730:exon
CAACCTACAGGACTAACCAA+CGG 0.675767 tig0023410:+71780 Msa1408730:CDS
GATCTCGGTTGGAAAGAACA+AGG 0.680183 tig0023410:+71324 Msa1408730:CDS
AATGAACTTTACCTGACCAG+TGG 0.705145 tig0023410:-71519 None:intergenic
GTTCGCCGGAGACAACACGC+CGG 0.804108 tig0023410:+70164 Msa1408730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATAAATATATTAACTTTA+AGG - tig0023410:72725-72744 None:intergenic 10.0%
!! ATTTGATATATCTTGATTTA+AGG + tig0023410:70846-70865 Msa1408730:intron 15.0%
!! ACATAAAAAATAATGAAACA+AGG - tig0023410:71600-71619 None:intergenic 15.0%
!! TTCAATTTATGTATATATCA+AGG + tig0023410:72299-72318 Msa1408730:CDS 15.0%
!! TCAATTTATGTATATATCAA+GGG + tig0023410:72300-72319 Msa1408730:CDS 15.0%
!!! ATTTGGAATTTTACTAATGA+TGG + tig0023410:70778-70797 Msa1408730:intron 20.0%
!!! GTTTTTTTTTTTTGTCCTTT+TGG + tig0023410:71005-71024 Msa1408730:intron 20.0%
!! AAAAAATAATGAAACAAGGT+TGG - tig0023410:71596-71615 None:intergenic 20.0%
!!! TGTTGTTTTCATATATAGAA+AGG - tig0023410:72061-72080 None:intergenic 20.0%
!! TTCTATATATGAAAACAACA+AGG + tig0023410:72061-72080 Msa1408730:CDS 20.0%
!! TCTATATATGAAAACAACAA+GGG + tig0023410:72062-72081 Msa1408730:CDS 20.0%
!!! TGACATAATTTTTCATACAT+TGG + tig0023410:72754-72773 Msa1408730:three_prime_UTR 20.0%
!! TTCTAAGAGATTGTGTAATT+TGG + tig0023410:70761-70780 Msa1408730:intron 25.0%
!!! TACTAATGATGGATTTTGTA+CGG + tig0023410:70789-70808 Msa1408730:intron 25.0%
! AAACTGAAAGATAATCTTCT+TGG - tig0023410:70881-70900 None:intergenic 25.0%
!!! TAGAACATAATTTGGTGTAA+GGG - tig0023410:71718-71737 None:intergenic 25.0%
!!! TTAGAACATAATTTGGTGTA+AGG - tig0023410:71719-71738 None:intergenic 25.0%
! TTACACCAAATTATGTTCTA+AGG + tig0023410:71718-71737 Msa1408730:CDS 25.0%
!!! ACTCTTTTTTATTTTTCCGT+TGG - tig0023410:71799-71818 None:intergenic 25.0%
! GGAAAAATAAAAAAGAGTGA+TGG + tig0023410:71801-71820 Msa1408730:CDS 25.0%
!!! TTACATCTTCTGAAGTTAAA+AGG - tig0023410:72004-72023 None:intergenic 25.0%
! GATGAGAACAAAATAATCAT+TGG + tig0023410:72194-72213 Msa1408730:CDS 25.0%
! AAGAACTGACATTATTGTAA+GGG - tig0023410:72411-72430 None:intergenic 25.0%
! CTCAAAAGATCTATTAAAAC+TGG - tig0023410:72482-72501 None:intergenic 25.0%
!!! CAGTTTTAATAGATCTTTTG+AGG + tig0023410:72480-72499 Msa1408730:CDS 25.0%
! GAAAGAAGAAAAGAATTAAC+TGG - tig0023410:72835-72854 None:intergenic 25.0%
TCATTTGTTCGAAGAGATTA+CGG + tig0023410:70317-70336 Msa1408730:CDS 30.0%
!!! ACTAATGATGGATTTTGTAC+GGG + tig0023410:70790-70809 Msa1408730:intron 30.0%
AGGTAGAATTTGGTTAAATG+TGG + tig0023410:70983-71002 Msa1408730:intron 30.0%
!! CTGATATTGTCTACTTTTCT+AGG + tig0023410:71073-71092 Msa1408730:intron 30.0%
!! ATATTGTCTACTTTTCTAGG+TGG + tig0023410:71076-71095 Msa1408730:intron 30.0%
TTTAATCGATAAGGAAACCT+CGG - tig0023410:71366-71385 None:intergenic 30.0%
CTGAAAGTCAAGAAATTTCT+GGG + tig0023410:71393-71412 Msa1408730:CDS 30.0%
! GGTAAAGTTCATTTTAGATG+AGG + tig0023410:71529-71548 Msa1408730:intron 30.0%
AGACTCCTTAGAACATAATT+TGG - tig0023410:71726-71745 None:intergenic 30.0%
GTTAAAAGGTTGACTAAAAC+TGG - tig0023410:71990-72009 None:intergenic 30.0%
ATGTATATATCAAGGGAACA+AGG + tig0023410:72307-72326 Msa1408730:CDS 30.0%
TGTATATATCAAGGGAACAA+GGG + tig0023410:72308-72327 Msa1408730:CDS 30.0%
CAAGAACTGACATTATTGTA+AGG - tig0023410:72412-72431 None:intergenic 30.0%
TATTAAAACTGGTATAGTGC+TGG - tig0023410:72471-72490 None:intergenic 30.0%
!! TTAATAGATCTTTTGAGGAC+TGG + tig0023410:72485-72504 Msa1408730:CDS 30.0%
AAGAATTAACTGGATCAATG+AGG - tig0023410:72825-72844 None:intergenic 30.0%
AACAAGACTAACAAATCAAG+AGG - tig0023410:72954-72973 None:intergenic 30.0%
! GAGGTTTTTGATGAGACTTA+TGG - tig0023410:70093-70112 None:intergenic 35.0%
ATCCTTTCTGAACATTGCAT+CGG - tig0023410:70262-70281 None:intergenic 35.0%
AATGAGTAAGAAGAGAGATC+CGG - tig0023410:70302-70321 None:intergenic 35.0%
!!! AAGCTCGTTTTATTTGTCTG+TGG + tig0023410:70505-70524 Msa1408730:intron 35.0%
ACAAACAAATCGAAACGACA+AGG - tig0023410:70618-70637 None:intergenic 35.0%
GAGATATCCAAATCGTCATA+CGG - tig0023410:70946-70965 None:intergenic 35.0%
! GTATGACGATTTGGATATCT+CGG + tig0023410:70945-70964 Msa1408730:CDS 35.0%
GTCAAAGAACAGGTAGAATT+TGG + tig0023410:70973-70992 Msa1408730:intron 35.0%
TACAGTATCGTCAATCCAAA+AGG - tig0023410:71023-71042 None:intergenic 35.0%
!! TCTACTTTTCTAGGTGGATT+TGG + tig0023410:71082-71101 Msa1408730:intron 35.0%
TAGCTGTCCTGAAAATCTAT+CGG + tig0023410:71244-71263 Msa1408730:CDS 35.0%
AGCATTCCTAAAAGCAATGA+AGG + tig0023410:71275-71294 Msa1408730:CDS 35.0%
GCATTCCTAAAAGCAATGAA+GGG + tig0023410:71276-71295 Msa1408730:CDS 35.0%
!! CTTTTCCCTTCATTGCTTTT+AGG - tig0023410:71284-71303 None:intergenic 35.0%
!! AGGAGCAGTTTTAATCGATA+AGG - tig0023410:71375-71394 None:intergenic 35.0%
CCAGAAATTTCTTGACTTTC+AGG - tig0023410:71395-71414 None:intergenic 35.0%
CCTGAAAGTCAAGAAATTTC+TGG + tig0023410:71392-71411 Msa1408730:CDS 35.0%
ATAGGGCAAAGAAAATCTTC+AGG - tig0023410:71464-71483 None:intergenic 35.0%
! TTTTCTTTGCCCTATATCGT+TGG + tig0023410:71469-71488 Msa1408730:CDS 35.0%
TCATTAGCTCCAACGATATA+GGG - tig0023410:71481-71500 None:intergenic 35.0%
TATATCGTTGGAGCTAATGA+GGG + tig0023410:71481-71500 Msa1408730:CDS 35.0%
!! TCTTTTTAGCCTTTCACTTG+CGG + tig0023410:72172-72191 Msa1408730:CDS 35.0%
AAAATAATCATTGGAGCCTC+AGG + tig0023410:72203-72222 Msa1408730:CDS 35.0%
! TTGGTAGATTTGCTCCAAAA+TGG + tig0023410:72239-72258 Msa1408730:CDS 35.0%
TCCAAAATGGAAGTCTTAGA+GGG + tig0023410:72252-72271 Msa1408730:CDS 35.0%
CACAGATTCAAGCAAATCAA+TGG + tig0023410:72376-72395 Msa1408730:CDS 35.0%
!!! TATAGTGCTGGCTTTTACAA+TGG - tig0023410:72459-72478 None:intergenic 35.0%
CTTGCACAAAGCAAGTAAAA+TGG - tig0023410:72540-72559 None:intergenic 35.0%
! TTTTACTTGCTTTGTGCAAG+AGG + tig0023410:72540-72559 Msa1408730:CDS 35.0%
TTTACTTGCTTTGTGCAAGA+GGG + tig0023410:72541-72560 Msa1408730:CDS 35.0%
CAAACTGATCAGATCAATTG+AGG + tig0023410:72781-72800 Msa1408730:three_prime_UTR 35.0%
GGTTGAAAAGGAACAATACA+GGG - tig0023410:72857-72876 None:intergenic 35.0%
AGGTTGAAAAGGAACAATAC+AGG - tig0023410:72858-72877 None:intergenic 35.0%
AGATTCTCAAGCATATAGCA+TGG + tig0023410:72995-73014 Msa1408730:three_prime_UTR 35.0%
AAGTCAGAGAGAGTCTAATG+AGG - tig0023410:70112-70131 None:intergenic 40.0%
! CCATTGTATTTGCTGGATTG+TGG - tig0023410:70137-70156 None:intergenic 40.0%
CCACAATCCAGCAAATACAA+TGG + tig0023410:70134-70153 Msa1408730:exon 40.0%
AATCCAGCAAATACAATGGC+CGG + tig0023410:70138-70157 Msa1408730:exon 40.0%
TGATGTCGTGAATTAAGCAC+AGG - tig0023410:70194-70213 None:intergenic 40.0%
TGCTTAATTCACGACATCAC+CGG + tig0023410:70195-70214 Msa1408730:CDS 40.0%
AGAGATTACGGAGCTGAATA+AGG + tig0023410:70329-70348 Msa1408730:CDS 40.0%
TTTCGAATTCAGAGGATTCG+TGG + tig0023410:70385-70404 Msa1408730:CDS 40.0%
CAACATAGAGGAATACACAC+TGG - tig0023410:70691-70710 None:intergenic 40.0%
GTGTGTATTCCTCTATGTTG+TGG + tig0023410:70691-70710 Msa1408730:intron 40.0%
CCAATACATCCACAACATAG+AGG - tig0023410:70703-70722 None:intergenic 40.0%
CCTCTATGTTGTGGATGTAT+TGG + tig0023410:70700-70719 Msa1408730:intron 40.0%
CAGTTTACCGTATGACGATT+TGG + tig0023410:70936-70955 Msa1408730:CDS 40.0%
AAACCAATTGAGACGAGCAA+CGG + tig0023410:71109-71128 Msa1408730:CDS 40.0%
AGACGAGCAACGGATAAATA+CGG + tig0023410:71119-71138 Msa1408730:CDS 40.0%
CAAGAAATCAGTCAGGTTCT+TGG + tig0023410:71191-71210 Msa1408730:CDS 40.0%
! CCTAGTTCCGATAGATTTTC+AGG - tig0023410:71254-71273 None:intergenic 40.0%
CCTGAAAATCTATCGGAACT+AGG + tig0023410:71251-71270 Msa1408730:CDS 40.0%
! AGTCAAGAAATTTCTGGGAG+TGG + tig0023410:71398-71417 Msa1408730:CDS 40.0%
! GTCAAGAAATTTCTGGGAGT+GGG + tig0023410:71399-71418 Msa1408730:CDS 40.0%
!!! GCATCACGCTTTTTAACTTC+AGG - tig0023410:71431-71450 None:intergenic 40.0%
CTCATTAGCTCCAACGATAT+AGG - tig0023410:71482-71501 None:intergenic 40.0%
CTATATCGTTGGAGCTAATG+AGG + tig0023410:71480-71499 Msa1408730:CDS 40.0%
AATGAACTTTACCTGACCAG+TGG - tig0023410:71522-71541 None:intergenic 40.0%
ATCGATCCATCTCTGTATGT+AGG - tig0023410:71646-71665 None:intergenic 40.0%
ATACAGAGATGGATCGATTG+TGG + tig0023410:71648-71667 Msa1408730:CDS 40.0%
TTGGTGTAAGGGTCAAATGT+TGG - tig0023410:71707-71726 None:intergenic 40.0%
!! TAAGGAGTCTTGTTTCTCAG+TGG + tig0023410:71736-71755 Msa1408730:CDS 40.0%
! CCTATTGTCAGTGCTTCTTT+TGG - tig0023410:71940-71959 None:intergenic 40.0%
!! CCAAAAGAAGCACTGACAAT+AGG + tig0023410:71937-71956 Msa1408730:CDS 40.0%
AGGATACTAATTGCTGAAGC+TGG + tig0023410:71957-71976 Msa1408730:CDS 40.0%
TGTAATGACGCAAGAAAACG+CGG + tig0023410:72019-72038 Msa1408730:CDS 40.0%
AAGGGACTTATTATGCTTGC+TGG + tig0023410:72080-72099 Msa1408730:CDS 40.0%
CTCCAAAATGGAAGTCTTAG+AGG + tig0023410:72251-72270 Msa1408730:CDS 40.0%
!!! TCCCTCTAAGACTTCCATTT+TGG - tig0023410:72256-72275 None:intergenic 40.0%
TAATGAAGTAGCCTTCCGTT+TGG - tig0023410:72648-72667 None:intergenic 40.0%
ACGGAAGGCTACTTCATTAT+TGG + tig0023410:72649-72668 Msa1408730:CDS 40.0%
!! TACAACAGCTTTGAAATGCG+AGG + tig0023410:72687-72706 Msa1408730:exon 40.0%
ATTAACTGGATCAATGAGGC+TGG - tig0023410:72821-72840 None:intergenic 40.0%
AACTTTACACGCTGATACAG+AGG - tig0023410:72878-72897 None:intergenic 40.0%
TGAATTAAGCACAGGAGCTC+CGG - tig0023410:70186-70205 None:intergenic 45.0%
CTTTCTGAACATTGCATCGG+CGG - tig0023410:70259-70278 None:intergenic 45.0%
! CGATTCTGCTTCGAGTTCTT+CGG + tig0023410:70356-70375 Msa1408730:CDS 45.0%
! TGCTTCGAGTTCTTCGGTTA+CGG + tig0023410:70362-70381 Msa1408730:CDS 45.0%
GGTTACGGTTTCGAATTCAG+AGG + tig0023410:70377-70396 Msa1408730:CDS 45.0%
AAGAGCAAGCACTAGATCAG+AGG - tig0023410:70412-70431 None:intergenic 45.0%
GACAAAATCTCAGTGGATCG+TGG - tig0023410:70596-70615 None:intergenic 45.0%
CGACAAGGACAAAATCTCAG+TGG - tig0023410:70603-70622 None:intergenic 45.0%
CGGGCGTGTATATCTGTAAA+AGG + tig0023410:70809-70828 Msa1408730:intron 45.0%
CTCGGAAGAAGTCAAAGAAC+AGG + tig0023410:70963-70982 Msa1408730:CDS 45.0%
TATCCGTTGCTCGTCTCAAT+TGG - tig0023410:71115-71134 None:intergenic 45.0%
GACGAGCAACGGATAAATAC+GGG + tig0023410:71120-71139 Msa1408730:CDS 45.0%
GGCTGGGATGAATCAAAAGA+CGG - tig0023410:71164-71183 None:intergenic 45.0%
! TGACTGATTTCTTGAGCCAG+GGG - tig0023410:71185-71204 None:intergenic 45.0%
! CTGACTGATTTCTTGAGCCA+GGG - tig0023410:71186-71205 None:intergenic 45.0%
GTTCTTGGCAAATCTGTGAG+TGG + tig0023410:71206-71225 Msa1408730:CDS 45.0%
GATCTCGGTTGGAAAGAACA+AGG + tig0023410:71324-71343 Msa1408730:CDS 45.0%
AATGAGGGATCCCGTTATTG+TGG + tig0023410:71496-71515 Msa1408730:CDS 45.0%
AGCGATCCTACATACAGAGA+TGG + tig0023410:71637-71656 Msa1408730:CDS 45.0%
CAACCTACAGGACTAACCAA+CGG + tig0023410:71780-71799 Msa1408730:CDS 45.0%
! TTTCCGTTGGTTAGTCCTGT+AGG - tig0023410:71786-71805 None:intergenic 45.0%
GGATCGTTCAGAGATGTTAC+AGG + tig0023410:71822-71841 Msa1408730:CDS 45.0%
CAGCAATTGAAACTCTCGTC+CGG + tig0023410:71853-71872 Msa1408730:CDS 45.0%
CATGGAAGCAAGAGAAAACG+CGG + tig0023410:72142-72161 Msa1408730:CDS 45.0%
TGTTCTCATCCGCAAGTGAA+AGG - tig0023410:72184-72203 None:intergenic 45.0%
CAAAGCAGATATAGCTCCTG+AGG - tig0023410:72222-72241 None:intergenic 45.0%
CTCAGGAGCTATATCTGCTT+TGG + tig0023410:72220-72239 Msa1408730:CDS 45.0%
TGGAAGTCTTAGAGGGAAGA+AGG + tig0023410:72259-72278 Msa1408730:CDS 45.0%
TGGAAACCTTTGCCTCTTGA+TGG - tig0023410:72439-72458 None:intergenic 45.0%
CTCTCCTGTATCAGTAGACT+TGG + tig0023410:72575-72594 Msa1408730:CDS 45.0%
! CAGTTCCGAAAGAGGTATGA+CGG - tig0023410:72603-72622 None:intergenic 45.0%
CGCTGATACAGAGGTTGAAA+AGG - tig0023410:72869-72888 None:intergenic 45.0%
TCACCGGCCATTGTATTTGC+TGG - tig0023410:70144-70163 None:intergenic 50.0%
CGCCGATGCAATGTTCAGAA+AGG + tig0023410:70257-70276 Msa1408730:CDS 50.0%
! ATTGCACCGATCTAGTTCGC+CGG + tig0023410:70280-70299 Msa1408730:CDS 50.0%
AGAGATCCGGCGAACTAGAT+CGG - tig0023410:70289-70308 None:intergenic 50.0%
!! TTTTGATTCATCCCAGCCCC+TGG + tig0023410:71166-71185 Msa1408730:CDS 50.0%
! CCTGACTGATTTCTTGAGCC+AGG - tig0023410:71187-71206 None:intergenic 50.0%
CCTGGCTCAAGAAATCAGTC+AGG + tig0023410:71184-71203 Msa1408730:CDS 50.0%
AACCGAGATCCTGCTGCAAA+CGG - tig0023410:71314-71333 None:intergenic 50.0%
CGATAAGGAAACCTCGGAAG+AGG - tig0023410:71360-71379 None:intergenic 50.0%
TGACCAGTGGCCACAATAAC+GGG - tig0023410:71509-71528 None:intergenic 50.0%
CTGACCAGTGGCCACAATAA+CGG - tig0023410:71510-71529 None:intergenic 50.0%
!! CCTCTGCTGAGTTTTTGGAC+AGG - tig0023410:71682-71701 None:intergenic 50.0%
CCTGTCCAAAAACTCAGCAG+AGG + tig0023410:71679-71698 Msa1408730:CDS 50.0%
! TGGAGCCTCTGCTGAGTTTT+TGG - tig0023410:71687-71706 None:intergenic 50.0%
CACAACATCGAGCAACCTAC+AGG + tig0023410:71768-71787 Msa1408730:CDS 50.0%
CGGAGGACTTGAACAATGGA+TGG - tig0023410:72113-72132 None:intergenic 50.0%
AGCTCGGAGGACTTGAACAA+TGG - tig0023410:72117-72136 None:intergenic 50.0%
ATTGTTCAAGTCCTCCGAGC+TGG + tig0023410:72116-72135 Msa1408730:CDS 50.0%
TTGCTTCCATGGTTCCAGCT+CGG - tig0023410:72133-72152 None:intergenic 50.0%
! CGCGTTTTCTCTTGCTTCCA+TGG - tig0023410:72144-72163 None:intergenic 50.0%
GAACAAGGGCAGAGCCATAA+GGG + tig0023410:72322-72341 Msa1408730:CDS 50.0%
TGTGATGATCCCTGCCCTTA+TGG - tig0023410:72339-72358 None:intergenic 50.0%
AGTTCTTGCCAGCCATCAAG+AGG + tig0023410:72424-72443 Msa1408730:CDS 50.0%
! AACCTTTGCCTCTTGATGGC+TGG - tig0023410:72435-72454 None:intergenic 50.0%
GGCACCAAGTCTACTGATAC+AGG - tig0023410:72582-72601 None:intergenic 50.0%
!! TGGTGCCGTCATACCTCTTT+CGG + tig0023410:72595-72614 Msa1408730:CDS 50.0%
CGTCATACCTCTTTCGGAAC+TGG + tig0023410:72601-72620 Msa1408730:CDS 50.0%
GTTCTAGCCAGTTCCGAAAG+AGG - tig0023410:72611-72630 None:intergenic 50.0%
CTTTCGGAACTGGCTAGAAC+TGG + tig0023410:72611-72630 Msa1408730:CDS 50.0%
! GAACATTGCATCGGCGGTGA+TGG - tig0023410:70253-70272 None:intergenic 55.0%
TTCAGTTTCAGCGCGTGACG+TGG + tig0023410:70893-70912 Msa1408730:CDS 55.0%
TCAGCGCGTGACGTGGAAAT+TGG + tig0023410:70900-70919 Msa1408730:CDS 55.0%
GATTTCTTGAGCCAGGGGCT+GGG - tig0023410:71180-71199 None:intergenic 55.0%
TGATTTCTTGAGCCAGGGGC+TGG - tig0023410:71181-71200 None:intergenic 55.0%
ACCCGTTTGCAGCAGGATCT+CGG + tig0023410:71309-71328 Msa1408730:CDS 55.0%
ACCGAGATCCTGCTGCAAAC+GGG - tig0023410:71313-71332 None:intergenic 55.0%
GTTTGCAGCAGGATCTCGGT+TGG + tig0023410:71313-71332 Msa1408730:CDS 55.0%
GAGTATCCATGCCTCTTCCG+AGG + tig0023410:71346-71365 Msa1408730:CDS 55.0%
AGGAAACCTCGGAAGAGGCA+TGG - tig0023410:71355-71374 None:intergenic 55.0%
GATCCCGTTATTGTGGCCAC+TGG + tig0023410:71503-71522 Msa1408730:CDS 55.0%
CGTTATTGTGGCCACTGGTC+AGG + tig0023410:71508-71527 Msa1408730:CDS 55.0%
CTGACCTGCAGGAAAGCTTC+CGG - tig0023410:71875-71894 None:intergenic 55.0%
GCTTTCCTGCAGGTCAGTTG+AGG + tig0023410:71878-71897 Msa1408730:CDS 55.0%
ACACTCCTCAACTGACCTGC+AGG - tig0023410:71886-71905 None:intergenic 55.0%
GGAACAAGGGCAGAGCCATA+AGG + tig0023410:72321-72340 Msa1408730:CDS 55.0%
TGCCAGCCATCAAGAGGCAA+AGG + tig0023410:72430-72449 Msa1408730:CDS 55.0%
GCTGCGTTCTCTTTGTTGCG+AGG - tig0023410:72515-72534 None:intergenic 55.0%
CTGGTACAGAGCGAGCCAAA+CGG + tig0023410:72630-72649 Msa1408730:CDS 55.0%
TACAGAGCGAGCCAAACGGA+AGG + tig0023410:72634-72653 Msa1408730:CDS 55.0%
ACAATGGCCGGTGAGTTCGC+CGG + tig0023410:70150-70169 Msa1408730:exon 60.0%
GTTGTCTCCGGCGAACTCAC+CGG - tig0023410:70160-70179 None:intergenic 60.0%
GACATCACCGGAATGTGCGC+AGG + tig0023410:70207-70226 Msa1408730:CDS 60.0%
ATCACCGGAATGTGCGCAGG+AGG + tig0023410:70210-70229 Msa1408730:CDS 60.0%
TCCTCCTCCTGCGCACATTC+CGG - tig0023410:70217-70236 None:intergenic 60.0%
! CATTGCATCGGCGGTGATGG+AGG - tig0023410:70250-70269 None:intergenic 60.0%
AGCTGCAACCCGTTTGCAGC+AGG + tig0023410:71302-71321 Msa1408730:CDS 60.0%
TCGTCCGGAAGCTTTCCTGC+AGG + tig0023410:71868-71887 Msa1408730:CDS 60.0%
AGTCCTCCGAGCTGGAACCA+TGG + tig0023410:72124-72143 Msa1408730:CDS 60.0%
CTTCCATGGTTCCAGCTCGG+AGG - tig0023410:72130-72149 None:intergenic 60.0%
AAGGGCAGAGCCATAAGGGC+AGG + tig0023410:72326-72345 Msa1408730:CDS 60.0%
AGGGCAGAGCCATAAGGGCA+GGG + tig0023410:72327-72346 Msa1408730:CDS 60.0%
GTTCGCCGGAGACAACACGC+CGG + tig0023410:70164-70183 Msa1408730:CDS 65.0%
GAGCTCCGGCGTGTTGTCTC+CGG - tig0023410:70172-70191 None:intergenic 65.0%
ACCGGAATGTGCGCAGGAGG+AGG + tig0023410:70213-70232 Msa1408730:CDS 65.0%
GGAATGTGCGCAGGAGGAGG+AGG + tig0023410:70216-70235 Msa1408730:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0023410 gene 70026 73027 70026 ID=Msa1408730;Name=Msa1408730
tig0023410 mRNA 70026 73027 70026 ID=Msa1408730-mRNA-1;Parent=Msa1408730;Name=Msa1408730-mRNA-1;_AED=0.03;_eAED=0.03;_QI=127|1|1|1|1|1|4|327|652
tig0023410 exon 70026 70488 70026 ID=Msa1408730-mRNA-1:exon:25664;Parent=Msa1408730-mRNA-1
tig0023410 exon 70868 70984 70868 ID=Msa1408730-mRNA-1:exon:25665;Parent=Msa1408730-mRNA-1
tig0023410 exon 71095 71529 71095 ID=Msa1408730-mRNA-1:exon:25666;Parent=Msa1408730-mRNA-1
tig0023410 exon 71630 73027 71630 ID=Msa1408730-mRNA-1:exon:25667;Parent=Msa1408730-mRNA-1
tig0023410 five_prime_UTR 70026 70152 70026 ID=Msa1408730-mRNA-1:five_prime_utr;Parent=Msa1408730-mRNA-1
tig0023410 CDS 70153 70488 70153 ID=Msa1408730-mRNA-1:cds;Parent=Msa1408730-mRNA-1
tig0023410 CDS 70868 70984 70868 ID=Msa1408730-mRNA-1:cds;Parent=Msa1408730-mRNA-1
tig0023410 CDS 71095 71529 71095 ID=Msa1408730-mRNA-1:cds;Parent=Msa1408730-mRNA-1
tig0023410 CDS 71630 72700 71630 ID=Msa1408730-mRNA-1:cds;Parent=Msa1408730-mRNA-1
tig0023410 three_prime_UTR 72701 73027 72701 ID=Msa1408730-mRNA-1:three_prime_utr;Parent=Msa1408730-mRNA-1
Gene Sequence

>Msa1408730

ATGGCCGGTGAGTTCGCCGGAGACAACACGCCGGAGCTCCTGTGCTTAATTCACGACATCACCGGAATGTGCGCAGGAGGAGGAGGCGATTCTTCCTCCATCACCGCCGATGCAATGTTCAGAAAGGATTGCACCGATCTAGTTCGCCGGATCTCTCTTCTTACTCATTTGTTCGAAGAGATTACGGAGCTGAATAAGGCTAACGATTCTGCTTCGAGTTCTTCGGTTACGGTTTCGAATTCAGAGGATTCGTGGTCCTCTGATCTAGTGCTTGCTCTTCAATCTGCTAAACGATTGTTACATGAAGCGAAGAATTTCAGTTCAAATTCATCTTCTGATGAAGCTGCCAAGAAGATTATCTTTCAGTTTCAGCGCGTGACGTGGAAATTGGAGAAATTATTAAGCAGTTTACCGTATGACGATTTGGATATCTCGGAAGAAGTCAAAGAACAGGTGGATTTGGTGAGAAACCAATTGAGACGAGCAACGGATAAATACGGGTTTATGATTTCAAAAATGCCGTCTTTTGATTCATCCCAGCCCCTGGCTCAAGAAATCAGTCAGGTTCTTGGCAAATCTGTGAGTGGATTGCATAAACAACATAGCTGTCCTGAAAATCTATCGGAACTAGGCAGCATTCCTAAAAGCAATGAAGGGAAAAGCTGCAACCCGTTTGCAGCAGGATCTCGGTTGGAAAGAACAAGGAGTATCCATGCCTCTTCCGAGGTTTCCTTATCGATTAAAACTGCTCCTGAAAGTCAAGAAATTTCTGGGAGTGGGAATTTACCTGAAGTTAAAAAGCGTGATGCAATTGTAATTCCTGAAGATTTTCTTTGCCCTATATCGTTGGAGCTAATGAGGGATCCCGTTATTGTGGCCACTGGTCAGACATATGAGCGATCCTACATACAGAGATGGATCGATTGTGGAAATACAACCTGTCCAAAAACTCAGCAGAGGCTCCAACATTTGACCCTTACACCAAATTATGTTCTAAGGAGTCTTGTTTCTCAGTGGTGTATAGAGCACAACATCGAGCAACCTACAGGACTAACCAACGGAAAAATAAAAAAGAGTGATGGATCGTTCAGAGATGTTACAGGTGACATAGCAGCAATTGAAACTCTCGTCCGGAAGCTTTCCTGCAGGTCAGTTGAGGAGTGTAGAGCAGCTGTTGCTGAAATTCGATCACTGTCCAAAAGAAGCACTGACAATAGGATACTAATTGCTGAAGCTGGAGCAATTCCAGTTTTAGTCAACCTTTTAACTTCAGAAGATGTAATGACGCAAGAAAACGCGGTTACTTCAATTCTCAACCTTTCTATATATGAAAACAACAAGGGACTTATTATGCTTGCTGGTGCGATTCCATCCATTGTTCAAGTCCTCCGAGCTGGAACCATGGAAGCAAGAGAAAACGCGGCAGCAACTCTTTTTAGCCTTTCACTTGCGGATGAGAACAAAATAATCATTGGAGCCTCAGGAGCTATATCTGCTTTGGTAGATTTGCTCCAAAATGGAAGTCTTAGAGGGAAGAAGGATGCAGCAACAGCTTTGTTCAATTTATGTATATATCAAGGGAACAAGGGCAGAGCCATAAGGGCAGGGATCATCACAGCACTGTTGAACATGCTCACAGATTCAAGCAAATCAATGGTTGACGAAGCCCTTACAATAATGTCAGTTCTTGCCAGCCATCAAGAGGCAAAGGTTTCCATTGTAAAAGCCAGCACTATACCAGTTTTAATAGATCTTTTGAGGACTGGATTGCCTCGCAACAAAGAGAACGCAGCTGCCATTTTACTTGCTTTGTGCAAGAGGGATACTGATAATCTCTCCTGTATCAGTAGACTTGGTGCCGTCATACCTCTTTCGGAACTGGCTAGAACTGGTACAGAGCGAGCCAAACGGAAGGCTACTTCATTATTGGAGCATCTTCGTAAATTACAACAGCTTTGA
Protein sequence

>Msa1408730

MAGEFAGDNTPELLCLIHDITGMCAGGGGDSSSITADAMFRKDCTDLVRRISLLTHLFEEITELNKANDSASSSSVTVSNSEDSWSSDLVLALQSAKRLLHEAKNFSSNSSSDEAAKKIIFQFQRVTWKLEKLLSSLPYDDLDISEEVKEQVDLVRNQLRRATDKYGFMISKMPSFDSSQPLAQEISQVLGKSVSGLHKQHSCPENLSELGSIPKSNEGKSCNPFAAGSRLERTRSIHASSEVSLSIKTAPESQEISGSGNLPEVKKRDAIVIPEDFLCPISLELMRDPVIVATGQTYERSYIQRWIDCGNTTCPKTQQRLQHLTLTPNYVLRSLVSQWCIEHNIEQPTGLTNGKIKKSDGSFRDVTGDIAAIETLVRKLSCRSVEECRAAVAEIRSLSKRSTDNRILIAEAGAIPVLVNLLTSEDVMTQENAVTSILNLSIYENNKGLIMLAGAIPSIVQVLRAGTMEARENAAATLFSLSLADENKIIIGASGAISALVDLLQNGSLRGKKDAATALFNLCIYQGNKGRAIRAGIITALLNMLTDSSKSMVDEALTIMSVLASHQEAKVSIVKASTIPVLIDLLRTGLPRNKENAAAILLALCKRDTDNLSCISRLGAVIPLSELARTGTERAKRKATSLLEHLRKLQQL*