AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1410870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1410870 MtrunA17_Chr1g0199491 94.167 120 7 0 1 120 1 120 1.00e-78 231
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1410870 AT3G54826.1 45.349 172 89 3 19 187 48 217 2.25e-47 155

Find 68 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATGCAACTTATATATT+TGG 0.189539 tig0023710:-2570 None:intergenic
CGTGGATGGTTTGGTGAAAA+AGG 0.255637 tig0023710:+2429 Msa1410870:CDS
CCAAATATATAAGTTGCATT+AGG 0.263978 tig0023710:+2570 Msa1410870:exon
TCGTATATCTTTAATTGTTT+TGG 0.272862 tig0023710:+2742 Msa1410870:three_prime_UTR
TCGAACACGACACCTGGAAT+TGG 0.340435 tig0023710:+828 Msa1410870:five_prime_UTR
TTAGGGTAGCACTGAGATAT+TGG 0.352434 tig0023710:+2588 Msa1410870:three_prime_UTR
AGGAACTGTCGAGGACTTCC+TGG 0.374832 tig0023710:+2449 Msa1410870:CDS
CCTCGTTTCACAGACCTTGC+AGG 0.386818 tig0023710:-2313 None:intergenic
TTTGGTTTAATTTCAGGTTC+CGG 0.387050 tig0023710:-870 None:intergenic
CAAATATATAAGTTGCATTA+GGG 0.405122 tig0023710:+2571 Msa1410870:exon
TTATTGAAACACCCAATTCC+AGG 0.413871 tig0023710:-840 None:intergenic
GATCGCCACCCCATCTTTCA+AGG 0.433969 tig0023710:+1003 Msa1410870:CDS
CGAACACGACACCTGGAATT+GGG 0.442099 tig0023710:+829 Msa1410870:five_prime_UTR
CAGATGTGGAATTCTCAAAA+GGG 0.455469 tig0023710:+2101 Msa1410870:CDS
ACAGATGTGGAATTCTCAAA+AGG 0.456549 tig0023710:+2100 Msa1410870:CDS
GCAGATCACCGTGGATGGTT+TGG 0.456571 tig0023710:+2420 Msa1410870:CDS
AAAACCAGCAGAATCCTTGA+TGG 0.458100 tig0023710:-2247 None:intergenic
TCAGTAATGCTCATGGATGC+AGG 0.465309 tig0023710:-2216 None:intergenic
GAGGACTTCCTGGCTGCTCA+TGG 0.466122 tig0023710:+2459 Msa1410870:CDS
GAAGCTGACACCTTGAAGTC+TGG 0.478664 tig0023710:+2180 Msa1410870:CDS
GTTGTTCACACCAGACTTCA+AGG 0.488969 tig0023710:-2190 None:intergenic
TCTGCCATCAAGGATTCTGC+TGG 0.494878 tig0023710:+2243 Msa1410870:CDS
GAATTGGATACCTTGAAAGA+TGG 0.498600 tig0023710:-1013 None:intergenic
CTTCTTCTCCATGAGCAGCC+AGG 0.500025 tig0023710:-2467 None:intergenic
TACTGAGGACTCTGCCATCA+AGG 0.500426 tig0023710:+2233 Msa1410870:CDS
GCTACATTTGAAGATATAGC+TGG 0.503715 tig0023710:+2519 Msa1410870:CDS
ACTGAGATATTGGAAGGGTA+AGG 0.508106 tig0023710:+2598 Msa1410870:three_prime_UTR
CTCAAGACATGATCTTGCTA+TGG 0.511583 tig0023710:+2284 Msa1410870:CDS
GAGTTGTAATAGCAAGGTGC+GGG 0.518452 tig0023710:+2373 Msa1410870:CDS
TTGGTATCGAACACGACACC+TGG 0.530829 tig0023710:+822 None:intergenic
AATTGGATACCTTGAAAGAT+GGG 0.531994 tig0023710:-1012 None:intergenic
CTGAGATATTGGAAGGGTAA+GGG 0.534906 tig0023710:+2599 Msa1410870:three_prime_UTR
CATGGAGAAGAAGTTAAAAG+AGG 0.542287 tig0023710:+2477 Msa1410870:CDS
AGCTGGAAAACAATCTTCGA+AGG 0.543959 tig0023710:+2536 Msa1410870:CDS
TCACAATCAAAGATACAGTC+TGG 0.544731 tig0023710:+2789 Msa1410870:three_prime_UTR
GAGCCATGTCGGCGTTGGCG+AGG 0.548094 tig0023710:+938 Msa1410870:exon
TGAGATATTGGAAGGGTAAG+GGG 0.549948 tig0023710:+2600 Msa1410870:three_prime_UTR
TAGAAGAGCCATGTCGGCGT+TGG 0.558330 tig0023710:+933 Msa1410870:exon
GTGTTTCAATAAGAAACCTC+CGG 0.561718 tig0023710:+851 Msa1410870:five_prime_UTR
TGATTGCAGATCACCGTGGA+TGG 0.563664 tig0023710:+2415 Msa1410870:CDS
GGTAGCACTGAGATATTGGA+AGG 0.578138 tig0023710:+2592 Msa1410870:three_prime_UTR
AATGCAATATTAAACAGATG+TGG 0.585013 tig0023710:+2087 Msa1410870:CDS
AGAGAGGAGTTGTAATAGCA+AGG 0.587080 tig0023710:+2367 Msa1410870:CDS
AAACCTCGCCAACGCCGACA+TGG 0.594770 tig0023710:-941 None:intergenic
GCTGGAAAACAATCTTCGAA+GGG 0.604283 tig0023710:+2537 Msa1410870:CDS
AGAGTCCTCAGTAATGCTCA+TGG 0.606371 tig0023710:-2223 None:intergenic
TGTCGCGAATCTTATGAGAG+AGG 0.606733 tig0023710:+2351 Msa1410870:CDS
ATGGAGAAGAAGTTAAAAGA+GGG 0.608033 tig0023710:+2478 Msa1410870:CDS
CAGTAATGCTCATGGATGCA+GGG 0.610587 tig0023710:-2215 None:intergenic
AGATGTGGAATTCTCAAAAG+GGG 0.631652 tig0023710:+2102 Msa1410870:CDS
CATAGCAAGATCATGTCTTG+AGG 0.633026 tig0023710:-2283 None:intergenic
GGTTTAATTTCAGGTTCCGG+AGG 0.637095 tig0023710:-867 None:intergenic
GGAGTTGTAATAGCAAGGTG+CGG 0.638024 tig0023710:+2372 Msa1410870:CDS
GTTGTAATAGCAAGGTGCGG+GGG 0.639697 tig0023710:+2375 Msa1410870:CDS
GATGTGGAATTCTCAAAAGG+GGG 0.643468 tig0023710:+2103 Msa1410870:CDS
ATATCGGAATAATCAAGAGA+CGG 0.647337 tig0023710:-2692 None:intergenic
GGATACCTTGAAAGATGGGG+TGG 0.654493 tig0023710:-1008 None:intergenic
CAAGGTCTGTGAAACGAGGT+CGG 0.658074 tig0023710:+2317 Msa1410870:CDS
CCTGCAAGGTCTGTGAAACG+AGG 0.661684 tig0023710:+2313 Msa1410870:CDS
AGTTGTAATAGCAAGGTGCG+GGG 0.662112 tig0023710:+2374 Msa1410870:CDS
ATAGAATAGAAGAGCCATGT+CGG 0.667952 tig0023710:+927 Msa1410870:exon
TGCATCCATGAGCATTACTG+AGG 0.683608 tig0023710:+2218 Msa1410870:CDS
ATGTGGAATTCTCAAAAGGG+GGG 0.692100 tig0023710:+2104 Msa1410870:CDS
GTAGCACTGAGATATTGGAA+GGG 0.705370 tig0023710:+2593 Msa1410870:three_prime_UTR
CAATCAAAGATACAGTCTGG+TGG 0.711975 tig0023710:+2792 Msa1410870:three_prime_UTR
CACTTGATTGCAGATCACCG+TGG 0.734143 tig0023710:+2411 Msa1410870:CDS
ATTGGATACCTTGAAAGATG+GGG 0.738148 tig0023710:-1011 None:intergenic
ATTGATAGAAGAATGCCCAG+AGG 0.812226 tig0023710:-2067 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATTCTTTTAGTAATT+TGG + tig0023710:1098-1117 Msa1410870:intron 10.0%
!!! ATAATATTCTTTTAGTAATT+TGG + tig0023710:1098-1117 Msa1410870:intron 10.0%
!! ATATAACAAATAATGAAATG+AGG - tig0023710:1443-1462 None:intergenic 15.0%
!! ATATAACAAATAATGAAATG+AGG - tig0023710:1443-1462 None:intergenic 15.0%
!! TAAGTCAATTGAAACAATTA+TGG + tig0023710:1162-1181 Msa1410870:intron 20.0%
!! AAGTCAATTGAAACAATTAT+GGG + tig0023710:1163-1182 Msa1410870:intron 20.0%
!! GAATCAAATTGAAATAGAAA+AGG - tig0023710:1421-1440 None:intergenic 20.0%
!!! TTAATATTCCATTGTGTTTT+TGG + tig0023710:2037-2056 Msa1410870:intron 20.0%
!!! TAATATTCCATTGTGTTTTT+GGG + tig0023710:2038-2057 Msa1410870:intron 20.0%
!! CAAATATATAAGTTGCATTA+GGG + tig0023710:2571-2590 Msa1410870:exon 20.0%
!!! ATTTTTGCTATTTGTTTTTG+AGG + tig0023710:2631-2650 Msa1410870:three_prime_UTR 20.0%
!!! TCGTATATCTTTAATTGTTT+TGG + tig0023710:2742-2761 Msa1410870:three_prime_UTR 20.0%
!! TAAGTCAATTGAAACAATTA+TGG + tig0023710:1162-1181 Msa1410870:intron 20.0%
!! AAGTCAATTGAAACAATTAT+GGG + tig0023710:1163-1182 Msa1410870:intron 20.0%
!! GAATCAAATTGAAATAGAAA+AGG - tig0023710:1421-1440 None:intergenic 20.0%
!!! TTAATATTCCATTGTGTTTT+TGG + tig0023710:2037-2056 Msa1410870:intron 20.0%
!!! TAATATTCCATTGTGTTTTT+GGG + tig0023710:2038-2057 Msa1410870:intron 20.0%
!! CAAATATATAAGTTGCATTA+GGG + tig0023710:2571-2590 Msa1410870:exon 20.0%
!!! ATTTTTGCTATTTGTTTTTG+AGG + tig0023710:2631-2650 Msa1410870:three_prime_UTR 20.0%
!!! TCGTATATCTTTAATTGTTT+TGG + tig0023710:2742-2761 Msa1410870:three_prime_UTR 20.0%
! GAATAATGAAACCCTAAAAT+TGG - tig0023710:1075-1094 None:intergenic 25.0%
!!! TTTTAGTAATTTGGTTCAAC+TGG + tig0023710:1107-1126 Msa1410870:intron 25.0%
! TAAATTTAGTGAGACTGTTT+GGG - tig0023710:1240-1259 None:intergenic 25.0%
! ATAAATTTAGTGAGACTGTT+TGG - tig0023710:1241-1260 None:intergenic 25.0%
! TTCAAAGCCAAAATTGTTAA+AGG + tig0023710:1535-1554 Msa1410870:intron 25.0%
!! ATCATCTCCTTTAACAATTT+TGG - tig0023710:1545-1564 None:intergenic 25.0%
! CATTCATTCATCTATTAACA+CGG - tig0023710:1576-1595 None:intergenic 25.0%
! ATGAATGAATGAATAGATCA+AGG + tig0023710:1585-1604 Msa1410870:intron 25.0%
! CCAGAAAGTTTATTAATTCA+AGG - tig0023710:1751-1770 None:intergenic 25.0%
! CCTTGAATTAATAAACTTTC+TGG + tig0023710:1748-1767 Msa1410870:intron 25.0%
!! AGAATAATAGTGGAGAAAAT+AGG + tig0023710:1883-1902 Msa1410870:intron 25.0%
! AATGCAATATTAAACAGATG+TGG + tig0023710:2087-2106 Msa1410870:CDS 25.0%
! CCTAATGCAACTTATATATT+TGG - tig0023710:2573-2592 None:intergenic 25.0%
! CCAAATATATAAGTTGCATT+AGG + tig0023710:2570-2589 Msa1410870:exon 25.0%
!!! TGAGTGACACTTTTTAATAT+CGG - tig0023710:2711-2730 None:intergenic 25.0%
! GTGACAATGAAAAATATTTC+AGG - tig0023710:2773-2792 None:intergenic 25.0%
! GAATAATGAAACCCTAAAAT+TGG - tig0023710:1075-1094 None:intergenic 25.0%
!!! TTTTAGTAATTTGGTTCAAC+TGG + tig0023710:1107-1126 Msa1410870:intron 25.0%
! TAAATTTAGTGAGACTGTTT+GGG - tig0023710:1240-1259 None:intergenic 25.0%
! ATAAATTTAGTGAGACTGTT+TGG - tig0023710:1241-1260 None:intergenic 25.0%
! TTCAAAGCCAAAATTGTTAA+AGG + tig0023710:1535-1554 Msa1410870:intron 25.0%
!! ATCATCTCCTTTAACAATTT+TGG - tig0023710:1545-1564 None:intergenic 25.0%
! CATTCATTCATCTATTAACA+CGG - tig0023710:1576-1595 None:intergenic 25.0%
! ATGAATGAATGAATAGATCA+AGG + tig0023710:1585-1604 Msa1410870:intron 25.0%
! CCAGAAAGTTTATTAATTCA+AGG - tig0023710:1751-1770 None:intergenic 25.0%
! CCTTGAATTAATAAACTTTC+TGG + tig0023710:1748-1767 Msa1410870:intron 25.0%
!! AGAATAATAGTGGAGAAAAT+AGG + tig0023710:1883-1902 Msa1410870:intron 25.0%
! AATGCAATATTAAACAGATG+TGG + tig0023710:2087-2106 Msa1410870:CDS 25.0%
! CCTAATGCAACTTATATATT+TGG - tig0023710:2573-2592 None:intergenic 25.0%
! CCAAATATATAAGTTGCATT+AGG + tig0023710:2570-2589 Msa1410870:exon 25.0%
!!! TGAGTGACACTTTTTAATAT+CGG - tig0023710:2711-2730 None:intergenic 25.0%
! GTGACAATGAAAAATATTTC+AGG - tig0023710:2773-2792 None:intergenic 25.0%
!! TTTGGTTTAATTTCAGGTTC+CGG - tig0023710:873-892 None:intergenic 30.0%
!!! TGCGATTTTGGTTTAATTTC+AGG - tig0023710:879-898 None:intergenic 30.0%
! ACTCAATTTTTCTGCGATTT+TGG - tig0023710:891-910 None:intergenic 30.0%
AATTGGATACCTTGAAAGAT+GGG - tig0023710:1015-1034 None:intergenic 30.0%
TTGGAGAAAGAAGAGAAAAA+GGG - tig0023710:1056-1075 None:intergenic 30.0%
ATTGGAGAAAGAAGAGAAAA+AGG - tig0023710:1057-1076 None:intergenic 30.0%
! TCTCTTCTTTCTCCAATTTT+AGG + tig0023710:1060-1079 Msa1410870:intron 30.0%
! CTCTTCTTTCTCCAATTTTA+GGG + tig0023710:1061-1080 Msa1410870:intron 30.0%
AAATTTAGTGAGACTGTTTG+GGG - tig0023710:1239-1258 None:intergenic 30.0%
TTTACTACTTAGGTTCAGTT+TGG - tig0023710:1296-1315 None:intergenic 30.0%
!! TTAGTTGCTGTTTACTACTT+AGG - tig0023710:1306-1325 None:intergenic 30.0%
ATATTGACACTGCACAAAAA+TGG + tig0023710:1459-1478 Msa1410870:intron 30.0%
AGTAAGAAAAGGAATGTTGT+GGG - tig0023710:1632-1651 None:intergenic 30.0%
GAATCAAACAATGTGATAGA+CGG - tig0023710:1724-1743 None:intergenic 30.0%
! CTATTATTCTGAAGGTAACA+CGG - tig0023710:1873-1892 None:intergenic 30.0%
TGTTACCTTCAGAATAATAG+TGG + tig0023710:1873-1892 Msa1410870:intron 30.0%
TTTCTCCACTATTATTCTGA+AGG - tig0023710:1881-1900 None:intergenic 30.0%
AAGCTCTTGCATGAATAAAT+AGG - tig0023710:1957-1976 None:intergenic 30.0%
AATGAGAAAGACTTACACAT+GGG - tig0023710:2019-2038 None:intergenic 30.0%
TAATGAGAAAGACTTACACA+TGG - tig0023710:2020-2039 None:intergenic 30.0%
ATGGAGAAGAAGTTAAAAGA+GGG + tig0023710:2478-2497 Msa1410870:CDS 30.0%
!! GTTTTTGAGGAAACAATTGT+CGG + tig0023710:2644-2663 Msa1410870:three_prime_UTR 30.0%
ATATCGGAATAATCAAGAGA+CGG - tig0023710:2695-2714 None:intergenic 30.0%
!! TTTGGTTTAATTTCAGGTTC+CGG - tig0023710:873-892 None:intergenic 30.0%
!!! TGCGATTTTGGTTTAATTTC+AGG - tig0023710:879-898 None:intergenic 30.0%
! ACTCAATTTTTCTGCGATTT+TGG - tig0023710:891-910 None:intergenic 30.0%
AATTGGATACCTTGAAAGAT+GGG - tig0023710:1015-1034 None:intergenic 30.0%
TTGGAGAAAGAAGAGAAAAA+GGG - tig0023710:1056-1075 None:intergenic 30.0%
ATTGGAGAAAGAAGAGAAAA+AGG - tig0023710:1057-1076 None:intergenic 30.0%
! TCTCTTCTTTCTCCAATTTT+AGG + tig0023710:1060-1079 Msa1410870:intron 30.0%
! CTCTTCTTTCTCCAATTTTA+GGG + tig0023710:1061-1080 Msa1410870:intron 30.0%
AAATTTAGTGAGACTGTTTG+GGG - tig0023710:1239-1258 None:intergenic 30.0%
TTTACTACTTAGGTTCAGTT+TGG - tig0023710:1296-1315 None:intergenic 30.0%
!! TTAGTTGCTGTTTACTACTT+AGG - tig0023710:1306-1325 None:intergenic 30.0%
ATATTGACACTGCACAAAAA+TGG + tig0023710:1459-1478 Msa1410870:intron 30.0%
AGTAAGAAAAGGAATGTTGT+GGG - tig0023710:1632-1651 None:intergenic 30.0%
GAATCAAACAATGTGATAGA+CGG - tig0023710:1724-1743 None:intergenic 30.0%
! CTATTATTCTGAAGGTAACA+CGG - tig0023710:1873-1892 None:intergenic 30.0%
TGTTACCTTCAGAATAATAG+TGG + tig0023710:1873-1892 Msa1410870:intron 30.0%
TTTCTCCACTATTATTCTGA+AGG - tig0023710:1881-1900 None:intergenic 30.0%
AAGCTCTTGCATGAATAAAT+AGG - tig0023710:1957-1976 None:intergenic 30.0%
AATGAGAAAGACTTACACAT+GGG - tig0023710:2019-2038 None:intergenic 30.0%
TAATGAGAAAGACTTACACA+TGG - tig0023710:2020-2039 None:intergenic 30.0%
ATGGAGAAGAAGTTAAAAGA+GGG + tig0023710:2478-2497 Msa1410870:CDS 30.0%
!! GTTTTTGAGGAAACAATTGT+CGG + tig0023710:2644-2663 Msa1410870:three_prime_UTR 30.0%
ATATCGGAATAATCAAGAGA+CGG - tig0023710:2695-2714 None:intergenic 30.0%
TTATTGAAACACCCAATTCC+AGG - tig0023710:843-862 None:intergenic 35.0%
GTGTTTCAATAAGAAACCTC+CGG + tig0023710:851-870 Msa1410870:five_prime_UTR 35.0%
ATAGAATAGAAGAGCCATGT+CGG + tig0023710:927-946 Msa1410870:exon 35.0%
ATTGGATACCTTGAAAGATG+GGG - tig0023710:1014-1033 None:intergenic 35.0%
GAATTGGATACCTTGAAAGA+TGG - tig0023710:1016-1035 None:intergenic 35.0%
AGAGAGTTGAGATGATGAAT+TGG - tig0023710:1032-1051 None:intergenic 35.0%
TAGATAAGCTCGCTCAAATA+AGG + tig0023710:1266-1285 Msa1410870:intron 35.0%
CCATAAGTTCAACTGATCAA+GGG + tig0023710:1492-1511 Msa1410870:intron 35.0%
TCAACTGATCAAGGGAAATT+TGG + tig0023710:1500-1519 Msa1410870:intron 35.0%
GTTAAAGGAGATGATGACTT+TGG + tig0023710:1550-1569 Msa1410870:intron 35.0%
TGAATAGATCAAGGTGATGT+TGG + tig0023710:1594-1613 Msa1410870:intron 35.0%
GAGTAAGAAAAGGAATGTTG+TGG - tig0023710:1633-1652 None:intergenic 35.0%
TCTACCATGCCTATTATCTA+TGG + tig0023710:1662-1681 Msa1410870:intron 35.0%
CGTAAGTGTCCATAGATAAT+AGG - tig0023710:1674-1693 None:intergenic 35.0%
!! TTCTGGTTCATTAACTGATC+TGG + tig0023710:1765-1784 Msa1410870:intron 35.0%
CAAAGCAAACTTATGTGAAG+CGG + tig0023710:1808-1827 Msa1410870:intron 35.0%
ACAGATGTGGAATTCTCAAA+AGG + tig0023710:2100-2119 Msa1410870:CDS 35.0%
CAGATGTGGAATTCTCAAAA+GGG + tig0023710:2101-2120 Msa1410870:CDS 35.0%
AGATGTGGAATTCTCAAAAG+GGG + tig0023710:2102-2121 Msa1410870:CDS 35.0%
CATGGAGAAGAAGTTAAAAG+AGG + tig0023710:2477-2496 Msa1410870:CDS 35.0%
GCTACATTTGAAGATATAGC+TGG + tig0023710:2519-2538 Msa1410870:CDS 35.0%
TCACAATCAAAGATACAGTC+TGG + tig0023710:2789-2808 Msa1410870:three_prime_UTR 35.0%
TTATTGAAACACCCAATTCC+AGG - tig0023710:843-862 None:intergenic 35.0%
GTGTTTCAATAAGAAACCTC+CGG + tig0023710:851-870 Msa1410870:five_prime_UTR 35.0%
ATAGAATAGAAGAGCCATGT+CGG + tig0023710:927-946 Msa1410870:exon 35.0%
ATTGGATACCTTGAAAGATG+GGG - tig0023710:1014-1033 None:intergenic 35.0%
GAATTGGATACCTTGAAAGA+TGG - tig0023710:1016-1035 None:intergenic 35.0%
AGAGAGTTGAGATGATGAAT+TGG - tig0023710:1032-1051 None:intergenic 35.0%
TAGATAAGCTCGCTCAAATA+AGG + tig0023710:1266-1285 Msa1410870:intron 35.0%
CCATAAGTTCAACTGATCAA+GGG + tig0023710:1492-1511 Msa1410870:intron 35.0%
TCAACTGATCAAGGGAAATT+TGG + tig0023710:1500-1519 Msa1410870:intron 35.0%
GTTAAAGGAGATGATGACTT+TGG + tig0023710:1550-1569 Msa1410870:intron 35.0%
TGAATAGATCAAGGTGATGT+TGG + tig0023710:1594-1613 Msa1410870:intron 35.0%
GAGTAAGAAAAGGAATGTTG+TGG - tig0023710:1633-1652 None:intergenic 35.0%
TCTACCATGCCTATTATCTA+TGG + tig0023710:1662-1681 Msa1410870:intron 35.0%
CGTAAGTGTCCATAGATAAT+AGG - tig0023710:1674-1693 None:intergenic 35.0%
!! TTCTGGTTCATTAACTGATC+TGG + tig0023710:1765-1784 Msa1410870:intron 35.0%
CAAAGCAAACTTATGTGAAG+CGG + tig0023710:1808-1827 Msa1410870:intron 35.0%
ACAGATGTGGAATTCTCAAA+AGG + tig0023710:2100-2119 Msa1410870:CDS 35.0%
CAGATGTGGAATTCTCAAAA+GGG + tig0023710:2101-2120 Msa1410870:CDS 35.0%
AGATGTGGAATTCTCAAAAG+GGG + tig0023710:2102-2121 Msa1410870:CDS 35.0%
CATGGAGAAGAAGTTAAAAG+AGG + tig0023710:2477-2496 Msa1410870:CDS 35.0%
GCTACATTTGAAGATATAGC+TGG + tig0023710:2519-2538 Msa1410870:CDS 35.0%
TCACAATCAAAGATACAGTC+TGG + tig0023710:2789-2808 Msa1410870:three_prime_UTR 35.0%
TGTAACGTTTAACCTTAGGC+GGG - tig0023710:1373-1392 None:intergenic 40.0%
CTGTAACGTTTAACCTTAGG+CGG - tig0023710:1374-1393 None:intergenic 40.0%
GCACTGTAACGTTTAACCTT+AGG - tig0023710:1377-1396 None:intergenic 40.0%
TAAACGTTACAGTGCAAGCA+TGG + tig0023710:1382-1401 Msa1410870:intron 40.0%
GCCATAAGTTCAACTGATCA+AGG + tig0023710:1491-1510 Msa1410870:intron 40.0%
CCCTTGATCAGTTGAACTTA+TGG - tig0023710:1495-1514 None:intergenic 40.0%
AAAAGGAATGTTGTGGGAGA+GGG - tig0023710:1626-1645 None:intergenic 40.0%
AGTGAGTAGGGAGTAAGAAA+AGG - tig0023710:1643-1662 None:intergenic 40.0%
GTGTCCATAGATAATAGGCA+TGG - tig0023710:1669-1688 None:intergenic 40.0%
GTTATCAGCCCTTTACCTAT+TGG + tig0023710:1698-1717 Msa1410870:intron 40.0%
ATAGACGGCCAATAGGTAAA+GGG - tig0023710:1709-1728 None:intergenic 40.0%
! CACGGTTTGTTTCACGATTT+AGG - tig0023710:1855-1874 None:intergenic 40.0%
! GACATCAGATTGTTTAGAGC+AGG - tig0023710:1932-1951 None:intergenic 40.0%
ATTGATAGAAGAATGCCCAG+AGG - tig0023710:2070-2089 None:intergenic 40.0%
GATGTGGAATTCTCAAAAGG+GGG + tig0023710:2103-2122 Msa1410870:CDS 40.0%
ATGTGGAATTCTCAAAAGGG+GGG + tig0023710:2104-2123 Msa1410870:CDS 40.0%
AAAACCAGCAGAATCCTTGA+TGG - tig0023710:2250-2269 None:intergenic 40.0%
CATAGCAAGATCATGTCTTG+AGG - tig0023710:2286-2305 None:intergenic 40.0%
CTCAAGACATGATCTTGCTA+TGG + tig0023710:2284-2303 Msa1410870:CDS 40.0%
AGAGAGGAGTTGTAATAGCA+AGG + tig0023710:2367-2386 Msa1410870:CDS 40.0%
!! CTTTTTCACCAAACCATCCA+CGG - tig0023710:2431-2450 None:intergenic 40.0%
AGCTGGAAAACAATCTTCGA+AGG + tig0023710:2536-2555 Msa1410870:CDS 40.0%
GCTGGAAAACAATCTTCGAA+GGG + tig0023710:2537-2556 Msa1410870:CDS 40.0%
! TTAGGGTAGCACTGAGATAT+TGG + tig0023710:2588-2607 Msa1410870:three_prime_UTR 40.0%
! GTAGCACTGAGATATTGGAA+GGG + tig0023710:2593-2612 Msa1410870:three_prime_UTR 40.0%
ACTGAGATATTGGAAGGGTA+AGG + tig0023710:2598-2617 Msa1410870:three_prime_UTR 40.0%
CTGAGATATTGGAAGGGTAA+GGG + tig0023710:2599-2618 Msa1410870:three_prime_UTR 40.0%
TGAGATATTGGAAGGGTAAG+GGG + tig0023710:2600-2619 Msa1410870:three_prime_UTR 40.0%
CAATCAAAGATACAGTCTGG+TGG + tig0023710:2792-2811 Msa1410870:three_prime_UTR 40.0%
TGTAACGTTTAACCTTAGGC+GGG - tig0023710:1373-1392 None:intergenic 40.0%
CTGTAACGTTTAACCTTAGG+CGG - tig0023710:1374-1393 None:intergenic 40.0%
GCACTGTAACGTTTAACCTT+AGG - tig0023710:1377-1396 None:intergenic 40.0%
TAAACGTTACAGTGCAAGCA+TGG + tig0023710:1382-1401 Msa1410870:intron 40.0%
GCCATAAGTTCAACTGATCA+AGG + tig0023710:1491-1510 Msa1410870:intron 40.0%
CCCTTGATCAGTTGAACTTA+TGG - tig0023710:1495-1514 None:intergenic 40.0%
AAAAGGAATGTTGTGGGAGA+GGG - tig0023710:1626-1645 None:intergenic 40.0%
AGTGAGTAGGGAGTAAGAAA+AGG - tig0023710:1643-1662 None:intergenic 40.0%
GTGTCCATAGATAATAGGCA+TGG - tig0023710:1669-1688 None:intergenic 40.0%
GTTATCAGCCCTTTACCTAT+TGG + tig0023710:1698-1717 Msa1410870:intron 40.0%
ATAGACGGCCAATAGGTAAA+GGG - tig0023710:1709-1728 None:intergenic 40.0%
! CACGGTTTGTTTCACGATTT+AGG - tig0023710:1855-1874 None:intergenic 40.0%
! GACATCAGATTGTTTAGAGC+AGG - tig0023710:1932-1951 None:intergenic 40.0%
ATTGATAGAAGAATGCCCAG+AGG - tig0023710:2070-2089 None:intergenic 40.0%
GATGTGGAATTCTCAAAAGG+GGG + tig0023710:2103-2122 Msa1410870:CDS 40.0%
ATGTGGAATTCTCAAAAGGG+GGG + tig0023710:2104-2123 Msa1410870:CDS 40.0%
AAAACCAGCAGAATCCTTGA+TGG - tig0023710:2250-2269 None:intergenic 40.0%
CATAGCAAGATCATGTCTTG+AGG - tig0023710:2286-2305 None:intergenic 40.0%
CTCAAGACATGATCTTGCTA+TGG + tig0023710:2284-2303 Msa1410870:CDS 40.0%
AGAGAGGAGTTGTAATAGCA+AGG + tig0023710:2367-2386 Msa1410870:CDS 40.0%
!! CTTTTTCACCAAACCATCCA+CGG - tig0023710:2431-2450 None:intergenic 40.0%
AGCTGGAAAACAATCTTCGA+AGG + tig0023710:2536-2555 Msa1410870:CDS 40.0%
GCTGGAAAACAATCTTCGAA+GGG + tig0023710:2537-2556 Msa1410870:CDS 40.0%
! TTAGGGTAGCACTGAGATAT+TGG + tig0023710:2588-2607 Msa1410870:three_prime_UTR 40.0%
! GTAGCACTGAGATATTGGAA+GGG + tig0023710:2593-2612 Msa1410870:three_prime_UTR 40.0%
ACTGAGATATTGGAAGGGTA+AGG + tig0023710:2598-2617 Msa1410870:three_prime_UTR 40.0%
CTGAGATATTGGAAGGGTAA+GGG + tig0023710:2599-2618 Msa1410870:three_prime_UTR 40.0%
TGAGATATTGGAAGGGTAAG+GGG + tig0023710:2600-2619 Msa1410870:three_prime_UTR 40.0%
CAATCAAAGATACAGTCTGG+TGG + tig0023710:2792-2811 Msa1410870:three_prime_UTR 40.0%
!! GGTTTAATTTCAGGTTCCGG+AGG - tig0023710:870-889 None:intergenic 45.0%
! TTACTTGTTGAACCCGCCTA+AGG + tig0023710:1358-1377 Msa1410870:intron 45.0%
GAAAAGGAATGTTGTGGGAG+AGG - tig0023710:1627-1646 None:intergenic 45.0%
TAGGCATGGTAGAGTGAGTA+GGG - tig0023710:1655-1674 None:intergenic 45.0%
ATAGGCATGGTAGAGTGAGT+AGG - tig0023710:1656-1675 None:intergenic 45.0%
GATAGACGGCCAATAGGTAA+AGG - tig0023710:1710-1729 None:intergenic 45.0%
CAATGTGATAGACGGCCAAT+AGG - tig0023710:1716-1735 None:intergenic 45.0%
GATGCTGCCCAAAAACACAA+TGG - tig0023710:2048-2067 None:intergenic 45.0%
GTTGTTCACACCAGACTTCA+AGG - tig0023710:2193-2212 None:intergenic 45.0%
CAGTAATGCTCATGGATGCA+GGG - tig0023710:2218-2237 None:intergenic 45.0%
TCAGTAATGCTCATGGATGC+AGG - tig0023710:2219-2238 None:intergenic 45.0%
TGCATCCATGAGCATTACTG+AGG + tig0023710:2218-2237 Msa1410870:CDS 45.0%
AGAGTCCTCAGTAATGCTCA+TGG - tig0023710:2226-2245 None:intergenic 45.0%
! TGCTATGGTTTTCACCTGCA+AGG + tig0023710:2299-2318 Msa1410870:CDS 45.0%
TGTCGCGAATCTTATGAGAG+AGG + tig0023710:2351-2370 Msa1410870:CDS 45.0%
GGAGTTGTAATAGCAAGGTG+CGG + tig0023710:2372-2391 Msa1410870:CDS 45.0%
GAGTTGTAATAGCAAGGTGC+GGG + tig0023710:2373-2392 Msa1410870:CDS 45.0%
AGTTGTAATAGCAAGGTGCG+GGG + tig0023710:2374-2393 Msa1410870:CDS 45.0%
!! CGTGGATGGTTTGGTGAAAA+AGG + tig0023710:2429-2448 Msa1410870:CDS 45.0%
TGGTGAAAAAGGAACTGTCG+AGG + tig0023710:2440-2459 Msa1410870:CDS 45.0%
! GGTAGCACTGAGATATTGGA+AGG + tig0023710:2592-2611 Msa1410870:three_prime_UTR 45.0%
!! GGTTTAATTTCAGGTTCCGG+AGG - tig0023710:870-889 None:intergenic 45.0%
! TTACTTGTTGAACCCGCCTA+AGG + tig0023710:1358-1377 Msa1410870:intron 45.0%
GAAAAGGAATGTTGTGGGAG+AGG - tig0023710:1627-1646 None:intergenic 45.0%
TAGGCATGGTAGAGTGAGTA+GGG - tig0023710:1655-1674 None:intergenic 45.0%
ATAGGCATGGTAGAGTGAGT+AGG - tig0023710:1656-1675 None:intergenic 45.0%
GATAGACGGCCAATAGGTAA+AGG - tig0023710:1710-1729 None:intergenic 45.0%
CAATGTGATAGACGGCCAAT+AGG - tig0023710:1716-1735 None:intergenic 45.0%
GATGCTGCCCAAAAACACAA+TGG - tig0023710:2048-2067 None:intergenic 45.0%
GTTGTTCACACCAGACTTCA+AGG - tig0023710:2193-2212 None:intergenic 45.0%
CAGTAATGCTCATGGATGCA+GGG - tig0023710:2218-2237 None:intergenic 45.0%
TCAGTAATGCTCATGGATGC+AGG - tig0023710:2219-2238 None:intergenic 45.0%
TGCATCCATGAGCATTACTG+AGG + tig0023710:2218-2237 Msa1410870:CDS 45.0%
AGAGTCCTCAGTAATGCTCA+TGG - tig0023710:2226-2245 None:intergenic 45.0%
! TGCTATGGTTTTCACCTGCA+AGG + tig0023710:2299-2318 Msa1410870:CDS 45.0%
TGTCGCGAATCTTATGAGAG+AGG + tig0023710:2351-2370 Msa1410870:CDS 45.0%
GGAGTTGTAATAGCAAGGTG+CGG + tig0023710:2372-2391 Msa1410870:CDS 45.0%
GAGTTGTAATAGCAAGGTGC+GGG + tig0023710:2373-2392 Msa1410870:CDS 45.0%
AGTTGTAATAGCAAGGTGCG+GGG + tig0023710:2374-2393 Msa1410870:CDS 45.0%
!! CGTGGATGGTTTGGTGAAAA+AGG + tig0023710:2429-2448 Msa1410870:CDS 45.0%
TGGTGAAAAAGGAACTGTCG+AGG + tig0023710:2440-2459 Msa1410870:CDS 45.0%
! GGTAGCACTGAGATATTGGA+AGG + tig0023710:2592-2611 Msa1410870:three_prime_UTR 45.0%
TCGAACACGACACCTGGAAT+TGG + tig0023710:828-847 Msa1410870:five_prime_UTR 50.0%
CGAACACGACACCTGGAATT+GGG + tig0023710:829-848 Msa1410870:five_prime_UTR 50.0%
! CGTTGGCGAGGTTTTTACAG+AGG + tig0023710:950-969 Msa1410870:CDS 50.0%
GGATACCTTGAAAGATGGGG+TGG - tig0023710:1011-1030 None:intergenic 50.0%
!!! TGTTTTTGGGCAGCATCCTC+TGG + tig0023710:2051-2070 Msa1410870:intron 50.0%
!! GTTTTTGGGCAGCATCCTCT+GGG + tig0023710:2052-2071 Msa1410870:intron 50.0%
GAAGCTGACACCTTGAAGTC+TGG + tig0023710:2180-2199 Msa1410870:CDS 50.0%
TACTGAGGACTCTGCCATCA+AGG + tig0023710:2233-2252 Msa1410870:CDS 50.0%
TCTGCCATCAAGGATTCTGC+TGG + tig0023710:2243-2262 Msa1410870:CDS 50.0%
CAAGGTCTGTGAAACGAGGT+CGG + tig0023710:2317-2336 Msa1410870:CDS 50.0%
GTTGTAATAGCAAGGTGCGG+GGG + tig0023710:2375-2394 Msa1410870:CDS 50.0%
CACTTGATTGCAGATCACCG+TGG + tig0023710:2411-2430 Msa1410870:CDS 50.0%
TGATTGCAGATCACCGTGGA+TGG + tig0023710:2415-2434 Msa1410870:CDS 50.0%
TCGAACACGACACCTGGAAT+TGG + tig0023710:828-847 Msa1410870:five_prime_UTR 50.0%
CGAACACGACACCTGGAATT+GGG + tig0023710:829-848 Msa1410870:five_prime_UTR 50.0%
! CGTTGGCGAGGTTTTTACAG+AGG + tig0023710:950-969 Msa1410870:CDS 50.0%
GGATACCTTGAAAGATGGGG+TGG - tig0023710:1011-1030 None:intergenic 50.0%
!!! TGTTTTTGGGCAGCATCCTC+TGG + tig0023710:2051-2070 Msa1410870:intron 50.0%
!! GTTTTTGGGCAGCATCCTCT+GGG + tig0023710:2052-2071 Msa1410870:intron 50.0%
GAAGCTGACACCTTGAAGTC+TGG + tig0023710:2180-2199 Msa1410870:CDS 50.0%
TACTGAGGACTCTGCCATCA+AGG + tig0023710:2233-2252 Msa1410870:CDS 50.0%
TCTGCCATCAAGGATTCTGC+TGG + tig0023710:2243-2262 Msa1410870:CDS 50.0%
CAAGGTCTGTGAAACGAGGT+CGG + tig0023710:2317-2336 Msa1410870:CDS 50.0%
GTTGTAATAGCAAGGTGCGG+GGG + tig0023710:2375-2394 Msa1410870:CDS 50.0%
CACTTGATTGCAGATCACCG+TGG + tig0023710:2411-2430 Msa1410870:CDS 50.0%
TGATTGCAGATCACCGTGGA+TGG + tig0023710:2415-2434 Msa1410870:CDS 50.0%
TAGAAGAGCCATGTCGGCGT+TGG + tig0023710:933-952 Msa1410870:exon 55.0%
GATCGCCACCCCATCTTTCA+AGG + tig0023710:1003-1022 Msa1410870:CDS 55.0%
CCTCGTTTCACAGACCTTGC+AGG - tig0023710:2316-2335 None:intergenic 55.0%
CCTGCAAGGTCTGTGAAACG+AGG + tig0023710:2313-2332 Msa1410870:CDS 55.0%
GCAGATCACCGTGGATGGTT+TGG + tig0023710:2420-2439 Msa1410870:CDS 55.0%
AGGAACTGTCGAGGACTTCC+TGG + tig0023710:2449-2468 Msa1410870:CDS 55.0%
CTTCTTCTCCATGAGCAGCC+AGG - tig0023710:2470-2489 None:intergenic 55.0%
TAGAAGAGCCATGTCGGCGT+TGG + tig0023710:933-952 Msa1410870:exon 55.0%
GATCGCCACCCCATCTTTCA+AGG + tig0023710:1003-1022 Msa1410870:CDS 55.0%
CCTCGTTTCACAGACCTTGC+AGG - tig0023710:2316-2335 None:intergenic 55.0%
CCTGCAAGGTCTGTGAAACG+AGG + tig0023710:2313-2332 Msa1410870:CDS 55.0%
GCAGATCACCGTGGATGGTT+TGG + tig0023710:2420-2439 Msa1410870:CDS 55.0%
AGGAACTGTCGAGGACTTCC+TGG + tig0023710:2449-2468 Msa1410870:CDS 55.0%
CTTCTTCTCCATGAGCAGCC+AGG - tig0023710:2470-2489 None:intergenic 55.0%
AAACCTCGCCAACGCCGACA+TGG - tig0023710:944-963 None:intergenic 60.0%
GAGGACTTCCTGGCTGCTCA+TGG + tig0023710:2459-2478 Msa1410870:CDS 60.0%
AAACCTCGCCAACGCCGACA+TGG - tig0023710:944-963 None:intergenic 60.0%
GAGGACTTCCTGGCTGCTCA+TGG + tig0023710:2459-2478 Msa1410870:CDS 60.0%
!! GAGCCATGTCGGCGTTGGCG+AGG + tig0023710:938-957 Msa1410870:exon 70.0%
!! GAGCCATGTCGGCGTTGGCG+AGG + tig0023710:938-957 Msa1410870:exon 70.0%
Chromosome Type Strat End Strand Name
tig0023710 gene 827 2816 827 ID=Msa1410870;Name=Msa1410870
tig0023710 mRNA 827 2816 827 ID=Msa1410870-mRNA-1;Parent=Msa1410870;Name=Msa1410870-mRNA-1;_AED=0.00;_eAED=0.00;_QI=116|1|1|1|1|1|2|235|199
tig0023710 exon 827 1024 827 ID=Msa1410870-mRNA-1:exon:26782;Parent=Msa1410870-mRNA-1
tig0023710 exon 2064 2816 2064 ID=Msa1410870-mRNA-1:exon:26783;Parent=Msa1410870-mRNA-1
tig0023710 five_prime_UTR 827 942 827 ID=Msa1410870-mRNA-1:five_prime_utr;Parent=Msa1410870-mRNA-1
tig0023710 CDS 943 1024 943 ID=Msa1410870-mRNA-1:cds;Parent=Msa1410870-mRNA-1
tig0023710 CDS 2064 2581 2064 ID=Msa1410870-mRNA-1:cds;Parent=Msa1410870-mRNA-1
tig0023710 three_prime_UTR 2582 2816 2582 ID=Msa1410870-mRNA-1:three_prime_utr;Parent=Msa1410870-mRNA-1
Gene Sequence

>Msa1410870

ATGTCGGCGTTGGCGAGGTTTTTACAGAGGCGTTTCGTTGCAACTCAATCATTTCATCATGATCGCCACCCCATCTTTCAAGCATCCTCTGGGCATTCTTCTATCAATGCAATATTAAACAGATGTGGAATTCTCAAAAGGGGGGTCTCGACACAGACAAATCTAAATCAAAATATCTGTGAAGATGTAAAAATCAGTGAAGCTGACACCTTGAAGTCTGGTGTGAACAACGTCCCTGCATCCATGAGCATTACTGAGGACTCTGCCATCAAGGATTCTGCTGGTTTTAGTGTGAAAGTATCCTCAAGACATGATCTTGCTATGGTTTTCACCTGCAAGGTCTGTGAAACGAGGTCGGTAAAGACATTTTGTCGCGAATCTTATGAGAGAGGAGTTGTAATAGCAAGGTGCGGGGGATGTAATAATCTTCACTTGATTGCAGATCACCGTGGATGGTTTGGTGAAAAAGGAACTGTCGAGGACTTCCTGGCTGCTCATGGAGAAGAAGTTAAAAGAGGGTCAATTGATACACTGAATGCTACATTTGAAGATATAGCTGGAAAACAATCTTCGAAGGGTACAATTTCACCAAATATATAA
Protein sequence

>Msa1410870

MSALARFLQRRFVATQSFHHDRHPIFQASSGHSSINAILNRCGILKRGVSTQTNLNQNICEDVKISEADTLKSGVNNVPASMSITEDSAIKDSAGFSVKVSSRHDLAMVFTCKVCETRSVKTFCRESYERGVVIARCGGCNNLHLIADHRGWFGEKGTVEDFLAAHGEEVKRGSIDTLNATFEDIAGKQSSKGTISPNI*