AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1415800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1415800 MtrunA17_Chr1g0160841 93.443 122 8 0 1 122 812 933 1.30e-72 233
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1415800 AT3G10690.1 75.397 126 31 0 2 127 819 944 1.76e-57 191

Find 55 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAGGTGATATTAATTTAA+AGG 0.209160 tig0024288:+1846 Msa1415800:three_prime_UTR
CGCACTCGTTGCTGAGATTA+AGG 0.260557 tig0024288:-1512 None:intergenic
GACATTCGGAAACGGCTTAA+AGG 0.311337 tig0024288:-464 None:intergenic
TTCAAATTTACCTAGCAAAT+CGG 0.321204 tig0024288:-1316 None:intergenic
ATAAATATGTTACACATAAT+AGG 0.334970 tig0024288:-1714 None:intergenic
GCCCTTCTCTCAACAACCTT+TGG 0.339110 tig0024288:-1765 None:intergenic
GCGATATCAAGTTCTTCTTC+AGG 0.376114 tig0024288:-1537 None:intergenic
TTGAATCGAGTTGGTCTGAT+TGG 0.376657 tig0024288:+491 Msa1415800:CDS
CTTGCTGCCGTATTTGTAGT+AGG 0.379437 tig0024288:+627 Msa1415800:CDS
CCGAGAGTGGTCATGGAAAA+CGG 0.399828 tig0024288:+438 Msa1415800:CDS
TCAAATTTACCTAGCAAATC+GGG 0.414586 tig0024288:-1315 None:intergenic
CAGTCAACAGGATTAAAGTT+CGG 0.430790 tig0024288:+1276 Msa1415800:CDS
CATTCATCTACAACTAGCCT+TGG 0.436503 tig0024288:-1560 None:intergenic
GTAGTAGGATTCACATTAGC+AGG 0.444284 tig0024288:+642 Msa1415800:CDS
CTCGATTCAAAGAAGACATT+CGG 0.445097 tig0024288:-478 None:intergenic
CGAGAGTGGTCATGGAAAAC+GGG 0.445643 tig0024288:+439 Msa1415800:CDS
GAATTCAAATAGATGAGTAT+TGG 0.454773 tig0024288:+1898 Msa1415800:three_prime_UTR
GCCAAAGTGGTACAGTCAAC+AGG 0.456273 tig0024288:+1264 Msa1415800:CDS
AGTCAACAGGATTAAAGTTC+GGG 0.474659 tig0024288:+1277 Msa1415800:CDS
TCCTGTTGACTGTACCACTT+TGG 0.476525 tig0024288:-1265 None:intergenic
TGTGAATCCTACTACAAATA+CGG 0.493089 tig0024288:-634 None:intergenic
GAATCTTATTTATTCCAAGA+AGG 0.493742 tig0024288:+1938 Msa1415800:three_prime_UTR
GCCTAGGTACCTGTCTGACT+TGG 0.498599 tig0024288:+1600 Msa1415800:three_prime_UTR
TTCCAAAGGTTGTTGAGAGA+AGG 0.506256 tig0024288:+1763 Msa1415800:three_prime_UTR
TTTATTTCCGAGAGTGGTCA+TGG 0.508275 tig0024288:+431 Msa1415800:CDS
CAATTCAATCCCGATTTGCT+AGG 0.508806 tig0024288:+1306 Msa1415800:CDS
AAAGCCTCACCAAACAAGTC+TGG 0.511247 tig0024288:-1966 None:intergenic
TCGGAAATAAACAGCAGCCA+TGG 0.511612 tig0024288:-419 None:intergenic
CTGCTGTTTATTTCCGAGAG+TGG 0.516337 tig0024288:+425 Msa1415800:CDS
TTAGAAAAGTATACTTGCCT+AGG 0.522991 tig0024288:+1584 Msa1415800:three_prime_UTR
AAGACAAAATCCCCGCCCTT+CGG 0.526778 tig0024288:-1742 None:intergenic
TCAAAGAAGACATTCGGAAA+CGG 0.527255 tig0024288:-472 None:intergenic
ATGTCTTCTTTGAATCGAGT+TGG 0.529089 tig0024288:+482 Msa1415800:CDS
TCCAAAGGTTGTTGAGAGAA+GGG 0.534707 tig0024288:+1764 Msa1415800:three_prime_UTR
GGCTATACTCCAAGTCAGAC+AGG 0.546983 tig0024288:-1609 None:intergenic
AGTTGGTCTGATTGGATACA+AGG 0.550577 tig0024288:+499 Msa1415800:CDS
GTGGTTCTTGTTAGCCAAAG+TGG 0.551421 tig0024288:+1251 Msa1415800:CDS
ACCGCTTGATCTTGAATCCG+TGG 0.551535 tig0024288:+1646 Msa1415800:three_prime_UTR
GGGTAGTGATATGCTTTCAA+TGG 0.552302 tig0024288:+1667 Msa1415800:three_prime_UTR
CGGAAATAAACAGCAGCCAT+GGG 0.564360 tig0024288:-418 None:intergenic
ATTCATCTACAACTAGCCTT+GGG 0.566651 tig0024288:-1559 None:intergenic
TTGATGCGACTTGATCATGC+TGG 0.593063 tig0024288:+1472 Msa1415800:CDS
GAAAGAGCTACGATGGCCCA+TGG 0.602646 tig0024288:+402 Msa1415800:CDS
AAAACCAGACTTGTTTGGTG+AGG 0.602979 tig0024288:+1962 Msa1415800:three_prime_UTR
CTATTGAAAACACGCATCTG+AGG 0.611612 tig0024288:+1829 Msa1415800:three_prime_UTR
CCGCTTGATCTTGAATCCGT+GGG 0.612299 tig0024288:+1647 Msa1415800:three_prime_UTR
CACAGTGGAAAGAGCTACGA+TGG 0.621816 tig0024288:+395 None:intergenic
TGAAAGCATATCACTACCCA+CGG 0.632145 tig0024288:-1663 None:intergenic
GAAGAACTTGATATCGCCCA+AGG 0.634206 tig0024288:+1543 Msa1415800:CDS
TCCAAGTCAGACAGGTACCT+AGG 0.638354 tig0024288:-1601 None:intergenic
CCCACGGATTCAAGATCAAG+CGG 0.643900 tig0024288:-1647 None:intergenic
CTACTACAAATACGGCAGCA+AGG 0.662886 tig0024288:-626 None:intergenic
TGGTGAAAGTGATGAACAAG+TGG 0.674126 tig0024288:+1232 Msa1415800:CDS
GTTACACATAATAGGTAACG+AGG 0.736749 tig0024288:-1706 None:intergenic
ACACATAATAGGTAACGAGG+AGG 0.737689 tig0024288:-1703 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGTTTATCATTTATAATT+TGG + tig0024288:682-701 Msa1415800:intron 10.0%
!! GATTATTATATGTTAATATA+AGG + tig0024288:1039-1058 Msa1415800:intron 10.0%
!! TTAGTTTATCATTTATAATT+TGG + tig0024288:682-701 Msa1415800:intron 10.0%
!! GATTATTATATGTTAATATA+AGG + tig0024288:1039-1058 Msa1415800:intron 10.0%
!!! TAACTTGTTAAGTATTTATT+TGG - tig0024288:908-927 None:intergenic 15.0%
!! ATAAATATGTTACACATAAT+AGG - tig0024288:1717-1736 None:intergenic 15.0%
!!! TAACTTGTTAAGTATTTATT+TGG - tig0024288:908-927 None:intergenic 15.0%
!! ATAAATATGTTACACATAAT+AGG - tig0024288:1717-1736 None:intergenic 15.0%
!! CTGATCATTGTTTAAAAAAT+TGG + tig0024288:713-732 Msa1415800:intron 20.0%
!!! GATTCTTAATGGTATTTTTT+TGG - tig0024288:804-823 None:intergenic 20.0%
!! TTTATATCTTTACAAGTAGA+AGG + tig0024288:1011-1030 Msa1415800:intron 20.0%
!!! TCTGTAATGACTATTTTATT+TGG + tig0024288:1425-1444 Msa1415800:intron 20.0%
!!! CTATTTTATTTGGTGAATTT+TGG + tig0024288:1435-1454 Msa1415800:intron 20.0%
!! CTGATCATTGTTTAAAAAAT+TGG + tig0024288:713-732 Msa1415800:intron 20.0%
!!! GATTCTTAATGGTATTTTTT+TGG - tig0024288:804-823 None:intergenic 20.0%
!! TTTATATCTTTACAAGTAGA+AGG + tig0024288:1011-1030 Msa1415800:intron 20.0%
!!! TCTGTAATGACTATTTTATT+TGG + tig0024288:1425-1444 Msa1415800:intron 20.0%
!!! CTATTTTATTTGGTGAATTT+TGG + tig0024288:1435-1454 Msa1415800:intron 20.0%
! ATGAACAGTTTATTGTCAAA+AGG - tig0024288:550-569 None:intergenic 25.0%
! GATAAACTAAGTTACATTGA+GGG - tig0024288:672-691 None:intergenic 25.0%
! TGATAAACTAAGTTACATTG+AGG - tig0024288:673-692 None:intergenic 25.0%
! CATTGTTTAAAAAATTGGAC+TGG + tig0024288:718-737 Msa1415800:intron 25.0%
! TAGTGATTTAAGCAGAAAAT+TGG + tig0024288:757-776 Msa1415800:intron 25.0%
!!! AGTTTTTGACTGATTCTTAA+TGG - tig0024288:815-834 None:intergenic 25.0%
! ATTAAGAATCAGTCAAAAAC+TGG + tig0024288:814-833 Msa1415800:intron 25.0%
! TAGCAATATGTAATTATCAC+TGG + tig0024288:1116-1135 Msa1415800:intron 25.0%
! TTTGTAGCAAGAAGTAAAAT+CGG - tig0024288:1175-1194 None:intergenic 25.0%
! TTCAAATTTACCTAGCAAAT+CGG - tig0024288:1319-1338 None:intergenic 25.0%
! AATCTCAGAATCAAATAAGA+GGG - tig0024288:1363-1382 None:intergenic 25.0%
! TAATCTCAGAATCAAATAAG+AGG - tig0024288:1364-1383 None:intergenic 25.0%
!!! AACATATTTATTTTGTCCGA+AGG + tig0024288:1726-1745 Msa1415800:three_prime_UTR 25.0%
!!! ACATATTTATTTTGTCCGAA+GGG + tig0024288:1727-1746 Msa1415800:three_prime_UTR 25.0%
! CTGAGGTGATATTAATTTAA+AGG + tig0024288:1846-1865 Msa1415800:three_prime_UTR 25.0%
! GAATTCAAATAGATGAGTAT+TGG + tig0024288:1898-1917 Msa1415800:three_prime_UTR 25.0%
!! GAATCTTATTTATTCCAAGA+AGG + tig0024288:1938-1957 Msa1415800:three_prime_UTR 25.0%
! ATGAACAGTTTATTGTCAAA+AGG - tig0024288:550-569 None:intergenic 25.0%
! GATAAACTAAGTTACATTGA+GGG - tig0024288:672-691 None:intergenic 25.0%
! TGATAAACTAAGTTACATTG+AGG - tig0024288:673-692 None:intergenic 25.0%
! CATTGTTTAAAAAATTGGAC+TGG + tig0024288:718-737 Msa1415800:intron 25.0%
! TAGTGATTTAAGCAGAAAAT+TGG + tig0024288:757-776 Msa1415800:intron 25.0%
!!! AGTTTTTGACTGATTCTTAA+TGG - tig0024288:815-834 None:intergenic 25.0%
! ATTAAGAATCAGTCAAAAAC+TGG + tig0024288:814-833 Msa1415800:intron 25.0%
! TAGCAATATGTAATTATCAC+TGG + tig0024288:1116-1135 Msa1415800:intron 25.0%
! TTTGTAGCAAGAAGTAAAAT+CGG - tig0024288:1175-1194 None:intergenic 25.0%
! TTCAAATTTACCTAGCAAAT+CGG - tig0024288:1319-1338 None:intergenic 25.0%
! AATCTCAGAATCAAATAAGA+GGG - tig0024288:1363-1382 None:intergenic 25.0%
! TAATCTCAGAATCAAATAAG+AGG - tig0024288:1364-1383 None:intergenic 25.0%
!!! AACATATTTATTTTGTCCGA+AGG + tig0024288:1726-1745 Msa1415800:three_prime_UTR 25.0%
!!! ACATATTTATTTTGTCCGAA+GGG + tig0024288:1727-1746 Msa1415800:three_prime_UTR 25.0%
! CTGAGGTGATATTAATTTAA+AGG + tig0024288:1846-1865 Msa1415800:three_prime_UTR 25.0%
! GAATTCAAATAGATGAGTAT+TGG + tig0024288:1898-1917 Msa1415800:three_prime_UTR 25.0%
!! GAATCTTATTTATTCCAAGA+AGG + tig0024288:1938-1957 Msa1415800:three_prime_UTR 25.0%
GTTTATTGTCAAAAGGATCA+TGG - tig0024288:543-562 None:intergenic 30.0%
TGTGAATCCTACTACAAATA+CGG - tig0024288:637-656 None:intergenic 30.0%
AGTCAAAAACTGGTTAAAGT+TGG + tig0024288:824-843 Msa1415800:intron 30.0%
AGTAAAATCGGTACTTCTAA+AGG - tig0024288:1163-1182 None:intergenic 30.0%
AAAAATCATTGTCTCAACAG+AGG + tig0024288:1208-1227 Msa1415800:intron 30.0%
TCAAATTTACCTAGCAAATC+GGG - tig0024288:1318-1337 None:intergenic 30.0%
TAAGAGGGAAATATACAACA+TGG - tig0024288:1348-1367 None:intergenic 30.0%
!! TTATTTGGTGAATTTTGGTC+AGG + tig0024288:1440-1459 Msa1415800:intron 30.0%
!! TATTTGGTGAATTTTGGTCA+GGG + tig0024288:1441-1460 Msa1415800:intron 30.0%
! TTAGAAAAGTATACTTGCCT+AGG + tig0024288:1584-1603 Msa1415800:three_prime_UTR 30.0%
! GCTTTCTTTTGTACTATTGT+GGG + tig0024288:1984-2003 Msa1415800:three_prime_UTR 30.0%
! CTTTCTTTTGTACTATTGTG+GGG + tig0024288:1985-2004 Msa1415800:three_prime_UTR 30.0%
GTTTATTGTCAAAAGGATCA+TGG - tig0024288:543-562 None:intergenic 30.0%
TGTGAATCCTACTACAAATA+CGG - tig0024288:637-656 None:intergenic 30.0%
AGTCAAAAACTGGTTAAAGT+TGG + tig0024288:824-843 Msa1415800:intron 30.0%
AGTAAAATCGGTACTTCTAA+AGG - tig0024288:1163-1182 None:intergenic 30.0%
AAAAATCATTGTCTCAACAG+AGG + tig0024288:1208-1227 Msa1415800:intron 30.0%
TCAAATTTACCTAGCAAATC+GGG - tig0024288:1318-1337 None:intergenic 30.0%
TAAGAGGGAAATATACAACA+TGG - tig0024288:1348-1367 None:intergenic 30.0%
!! TTATTTGGTGAATTTTGGTC+AGG + tig0024288:1440-1459 Msa1415800:intron 30.0%
!! TATTTGGTGAATTTTGGTCA+GGG + tig0024288:1441-1460 Msa1415800:intron 30.0%
! TTAGAAAAGTATACTTGCCT+AGG + tig0024288:1584-1603 Msa1415800:three_prime_UTR 30.0%
TCAAAGAAGACATTCGGAAA+CGG - tig0024288:475-494 None:intergenic 35.0%
CTCGATTCAAAGAAGACATT+CGG - tig0024288:481-500 None:intergenic 35.0%
ATGTCTTCTTTGAATCGAGT+TGG + tig0024288:482-501 Msa1415800:CDS 35.0%
! ACCTATGTTTCAGTTTTCTG+CGG + tig0024288:596-615 Msa1415800:intron 35.0%
! ACATACGCACATGATTTTTC+TGG + tig0024288:933-952 Msa1415800:intron 35.0%
CAGTCAACAGGATTAAAGTT+CGG + tig0024288:1276-1295 Msa1415800:CDS 35.0%
AGTCAACAGGATTAAAGTTC+GGG + tig0024288:1277-1296 Msa1415800:CDS 35.0%
TAAGCTTATGACAAGTGTCT+TGG + tig0024288:1390-1409 Msa1415800:intron 35.0%
ATAGTCATTACAGAAGCAGT+AGG - tig0024288:1419-1438 None:intergenic 35.0%
!! ATTTGGTGAATTTTGGTCAG+GGG + tig0024288:1442-1461 Msa1415800:intron 35.0%
!! ATTCATCTACAACTAGCCTT+GGG - tig0024288:1562-1581 None:intergenic 35.0%
! AAGCGGTATCATTTTGTGAA+GGG - tig0024288:1633-1652 None:intergenic 35.0%
GTTACACATAATAGGTAACG+AGG - tig0024288:1709-1728 None:intergenic 35.0%
!!! TATTTATTTTGTCCGAAGGG+CGG + tig0024288:1730-1749 Msa1415800:three_prime_UTR 35.0%
! AAGGAAAAACCAGACTTGTT+TGG + tig0024288:1957-1976 Msa1415800:three_prime_UTR 35.0%
! GGCTTTCTTTTGTACTATTG+TGG + tig0024288:1983-2002 Msa1415800:three_prime_UTR 35.0%
TCAAAGAAGACATTCGGAAA+CGG - tig0024288:475-494 None:intergenic 35.0%
CTCGATTCAAAGAAGACATT+CGG - tig0024288:481-500 None:intergenic 35.0%
ATGTCTTCTTTGAATCGAGT+TGG + tig0024288:482-501 Msa1415800:CDS 35.0%
! ACCTATGTTTCAGTTTTCTG+CGG + tig0024288:596-615 Msa1415800:intron 35.0%
! ACATACGCACATGATTTTTC+TGG + tig0024288:933-952 Msa1415800:intron 35.0%
CAGTCAACAGGATTAAAGTT+CGG + tig0024288:1276-1295 Msa1415800:CDS 35.0%
AGTCAACAGGATTAAAGTTC+GGG + tig0024288:1277-1296 Msa1415800:CDS 35.0%
TAAGCTTATGACAAGTGTCT+TGG + tig0024288:1390-1409 Msa1415800:intron 35.0%
ATAGTCATTACAGAAGCAGT+AGG - tig0024288:1419-1438 None:intergenic 35.0%
!! ATTTGGTGAATTTTGGTCAG+GGG + tig0024288:1442-1461 Msa1415800:intron 35.0%
!! ATTCATCTACAACTAGCCTT+GGG - tig0024288:1562-1581 None:intergenic 35.0%
! AAGCGGTATCATTTTGTGAA+GGG - tig0024288:1633-1652 None:intergenic 35.0%
GTTACACATAATAGGTAACG+AGG - tig0024288:1709-1728 None:intergenic 35.0%
!!! TATTTATTTTGTCCGAAGGG+CGG + tig0024288:1730-1749 Msa1415800:three_prime_UTR 35.0%
! AAGGAAAAACCAGACTTGTT+TGG + tig0024288:1957-1976 Msa1415800:three_prime_UTR 35.0%
TTTATTTCCGAGAGTGGTCA+TGG + tig0024288:431-450 Msa1415800:CDS 40.0%
!! TTGAATCGAGTTGGTCTGAT+TGG + tig0024288:491-510 Msa1415800:CDS 40.0%
! AGTTGGTCTGATTGGATACA+AGG + tig0024288:499-518 Msa1415800:CDS 40.0%
AAAAGGATCATGGCAGTGAA+AGG - tig0024288:533-552 None:intergenic 40.0%
TCCGCAGAAAACTGAAACAT+AGG - tig0024288:600-619 None:intergenic 40.0%
GTAGTAGGATTCACATTAGC+AGG + tig0024288:642-661 Msa1415800:CDS 40.0%
!! TAATTATCACTGGTTCGCCA+GGG + tig0024288:1126-1145 Msa1415800:intron 40.0%
TAAAGGTTCAACTTCAACCC+TGG - tig0024288:1146-1165 None:intergenic 40.0%
ATCATTGTCTCAACAGAGGA+TGG + tig0024288:1212-1231 Msa1415800:intron 40.0%
TGGTGAAAGTGATGAACAAG+TGG + tig0024288:1232-1251 Msa1415800:CDS 40.0%
! CAATTCAATCCCGATTTGCT+AGG + tig0024288:1306-1325 Msa1415800:CDS 40.0%
GCGATATCAAGTTCTTCTTC+AGG - tig0024288:1540-1559 None:intergenic 40.0%
! CATTCATCTACAACTAGCCT+TGG - tig0024288:1563-1582 None:intergenic 40.0%
! CAAGCGGTATCATTTTGTGA+AGG - tig0024288:1634-1653 None:intergenic 40.0%
! TGAAAGCATATCACTACCCA+CGG - tig0024288:1666-1685 None:intergenic 40.0%
GGGTAGTGATATGCTTTCAA+TGG + tig0024288:1667-1686 Msa1415800:three_prime_UTR 40.0%
ACACATAATAGGTAACGAGG+AGG - tig0024288:1706-1725 None:intergenic 40.0%
!!! ATTTATTTTGTCCGAAGGGC+GGG + tig0024288:1731-1750 Msa1415800:three_prime_UTR 40.0%
TTCCAAAGGTTGTTGAGAGA+AGG + tig0024288:1763-1782 Msa1415800:three_prime_UTR 40.0%
TCCAAAGGTTGTTGAGAGAA+GGG + tig0024288:1764-1783 Msa1415800:three_prime_UTR 40.0%
CTATTGAAAACACGCATCTG+AGG + tig0024288:1829-1848 Msa1415800:three_prime_UTR 40.0%
!!! CAAGTCTGGTTTTTCCTTCT+TGG - tig0024288:1955-1974 None:intergenic 40.0%
! AAAACCAGACTTGTTTGGTG+AGG + tig0024288:1962-1981 Msa1415800:three_prime_UTR 40.0%
TTTATTTCCGAGAGTGGTCA+TGG + tig0024288:431-450 Msa1415800:CDS 40.0%
!! TTGAATCGAGTTGGTCTGAT+TGG + tig0024288:491-510 Msa1415800:CDS 40.0%
! AGTTGGTCTGATTGGATACA+AGG + tig0024288:499-518 Msa1415800:CDS 40.0%
AAAAGGATCATGGCAGTGAA+AGG - tig0024288:533-552 None:intergenic 40.0%
TCCGCAGAAAACTGAAACAT+AGG - tig0024288:600-619 None:intergenic 40.0%
GTAGTAGGATTCACATTAGC+AGG + tig0024288:642-661 Msa1415800:CDS 40.0%
!! TAATTATCACTGGTTCGCCA+GGG + tig0024288:1126-1145 Msa1415800:intron 40.0%
TAAAGGTTCAACTTCAACCC+TGG - tig0024288:1146-1165 None:intergenic 40.0%
ATCATTGTCTCAACAGAGGA+TGG + tig0024288:1212-1231 Msa1415800:intron 40.0%
TGGTGAAAGTGATGAACAAG+TGG + tig0024288:1232-1251 Msa1415800:CDS 40.0%
! CAATTCAATCCCGATTTGCT+AGG + tig0024288:1306-1325 Msa1415800:CDS 40.0%
GCGATATCAAGTTCTTCTTC+AGG - tig0024288:1540-1559 None:intergenic 40.0%
! CATTCATCTACAACTAGCCT+TGG - tig0024288:1563-1582 None:intergenic 40.0%
! CAAGCGGTATCATTTTGTGA+AGG - tig0024288:1634-1653 None:intergenic 40.0%
! TGAAAGCATATCACTACCCA+CGG - tig0024288:1666-1685 None:intergenic 40.0%
GGGTAGTGATATGCTTTCAA+TGG + tig0024288:1667-1686 Msa1415800:three_prime_UTR 40.0%
ACACATAATAGGTAACGAGG+AGG - tig0024288:1706-1725 None:intergenic 40.0%
!!! ATTTATTTTGTCCGAAGGGC+GGG + tig0024288:1731-1750 Msa1415800:three_prime_UTR 40.0%
TTCCAAAGGTTGTTGAGAGA+AGG + tig0024288:1763-1782 Msa1415800:three_prime_UTR 40.0%
TCCAAAGGTTGTTGAGAGAA+GGG + tig0024288:1764-1783 Msa1415800:three_prime_UTR 40.0%
CTATTGAAAACACGCATCTG+AGG + tig0024288:1829-1848 Msa1415800:three_prime_UTR 40.0%
!!! CAAGTCTGGTTTTTCCTTCT+TGG - tig0024288:1955-1974 None:intergenic 40.0%
! AAAACCAGACTTGTTTGGTG+AGG + tig0024288:1962-1981 Msa1415800:three_prime_UTR 40.0%
CGGAAATAAACAGCAGCCAT+GGG - tig0024288:421-440 None:intergenic 45.0%
TCGGAAATAAACAGCAGCCA+TGG - tig0024288:422-441 None:intergenic 45.0%
! CTGCTGTTTATTTCCGAGAG+TGG + tig0024288:425-444 Msa1415800:CDS 45.0%
GACATTCGGAAACGGCTTAA+AGG - tig0024288:467-486 None:intergenic 45.0%
CTACTACAAATACGGCAGCA+AGG - tig0024288:629-648 None:intergenic 45.0%
CTTGCTGCCGTATTTGTAGT+AGG + tig0024288:627-646 Msa1415800:CDS 45.0%
! TTGGACTGGACTAGACTAGA+TGG + tig0024288:732-751 Msa1415800:intron 45.0%
!! GTAATTATCACTGGTTCGCC+AGG + tig0024288:1125-1144 Msa1415800:intron 45.0%
GTGGTTCTTGTTAGCCAAAG+TGG + tig0024288:1251-1270 Msa1415800:CDS 45.0%
! TCCTGTTGACTGTACCACTT+TGG - tig0024288:1268-1287 None:intergenic 45.0%
TTGATGCGACTTGATCATGC+TGG + tig0024288:1472-1491 Msa1415800:CDS 45.0%
GAAGAACTTGATATCGCCCA+AGG + tig0024288:1543-1562 Msa1415800:CDS 45.0%
!!! TTTATTTTGTCCGAAGGGCG+GGG + tig0024288:1732-1751 Msa1415800:three_prime_UTR 45.0%
AAAGCCTCACCAAACAAGTC+TGG - tig0024288:1969-1988 None:intergenic 45.0%
CGGAAATAAACAGCAGCCAT+GGG - tig0024288:421-440 None:intergenic 45.0%
TCGGAAATAAACAGCAGCCA+TGG - tig0024288:422-441 None:intergenic 45.0%
! CTGCTGTTTATTTCCGAGAG+TGG + tig0024288:425-444 Msa1415800:CDS 45.0%
GACATTCGGAAACGGCTTAA+AGG - tig0024288:467-486 None:intergenic 45.0%
CTACTACAAATACGGCAGCA+AGG - tig0024288:629-648 None:intergenic 45.0%
CTTGCTGCCGTATTTGTAGT+AGG + tig0024288:627-646 Msa1415800:CDS 45.0%
! TTGGACTGGACTAGACTAGA+TGG + tig0024288:732-751 Msa1415800:intron 45.0%
!! GTAATTATCACTGGTTCGCC+AGG + tig0024288:1125-1144 Msa1415800:intron 45.0%
GTGGTTCTTGTTAGCCAAAG+TGG + tig0024288:1251-1270 Msa1415800:CDS 45.0%
! TCCTGTTGACTGTACCACTT+TGG - tig0024288:1268-1287 None:intergenic 45.0%
TTGATGCGACTTGATCATGC+TGG + tig0024288:1472-1491 Msa1415800:CDS 45.0%
GAAGAACTTGATATCGCCCA+AGG + tig0024288:1543-1562 Msa1415800:CDS 45.0%
!!! TTTATTTTGTCCGAAGGGCG+GGG + tig0024288:1732-1751 Msa1415800:three_prime_UTR 45.0%
AAAGCCTCACCAAACAAGTC+TGG - tig0024288:1969-1988 None:intergenic 45.0%
! CCGTTTTCCATGACCACTCT+CGG - tig0024288:441-460 None:intergenic 50.0%
CCGAGAGTGGTCATGGAAAA+CGG + tig0024288:438-457 Msa1415800:CDS 50.0%
CGAGAGTGGTCATGGAAAAC+GGG + tig0024288:439-458 Msa1415800:CDS 50.0%
GCCAAAGTGGTACAGTCAAC+AGG + tig0024288:1264-1283 Msa1415800:CDS 50.0%
CGCACTCGTTGCTGAGATTA+AGG - tig0024288:1515-1534 None:intergenic 50.0%
TCCAAGTCAGACAGGTACCT+AGG - tig0024288:1604-1623 None:intergenic 50.0%
GGCTATACTCCAAGTCAGAC+AGG - tig0024288:1612-1631 None:intergenic 50.0%
ACCGCTTGATCTTGAATCCG+TGG + tig0024288:1646-1665 Msa1415800:three_prime_UTR 50.0%
CCCACGGATTCAAGATCAAG+CGG - tig0024288:1650-1669 None:intergenic 50.0%
CCGCTTGATCTTGAATCCGT+GGG + tig0024288:1647-1666 Msa1415800:three_prime_UTR 50.0%
AAGACAAAATCCCCGCCCTT+CGG - tig0024288:1745-1764 None:intergenic 50.0%
! GCGGGGATTTTGTCTTCCAA+AGG + tig0024288:1749-1768 Msa1415800:three_prime_UTR 50.0%
GCCCTTCTCTCAACAACCTT+TGG - tig0024288:1768-1787 None:intergenic 50.0%
! CCGTTTTCCATGACCACTCT+CGG - tig0024288:441-460 None:intergenic 50.0%
CCGAGAGTGGTCATGGAAAA+CGG + tig0024288:438-457 Msa1415800:CDS 50.0%
CGAGAGTGGTCATGGAAAAC+GGG + tig0024288:439-458 Msa1415800:CDS 50.0%
GCCAAAGTGGTACAGTCAAC+AGG + tig0024288:1264-1283 Msa1415800:CDS 50.0%
CGCACTCGTTGCTGAGATTA+AGG - tig0024288:1515-1534 None:intergenic 50.0%
TCCAAGTCAGACAGGTACCT+AGG - tig0024288:1604-1623 None:intergenic 50.0%
GGCTATACTCCAAGTCAGAC+AGG - tig0024288:1612-1631 None:intergenic 50.0%
ACCGCTTGATCTTGAATCCG+TGG + tig0024288:1646-1665 Msa1415800:three_prime_UTR 50.0%
CCCACGGATTCAAGATCAAG+CGG - tig0024288:1650-1669 None:intergenic 50.0%
CCGCTTGATCTTGAATCCGT+GGG + tig0024288:1647-1666 Msa1415800:three_prime_UTR 50.0%
AAGACAAAATCCCCGCCCTT+CGG - tig0024288:1745-1764 None:intergenic 50.0%
! GCGGGGATTTTGTCTTCCAA+AGG + tig0024288:1749-1768 Msa1415800:three_prime_UTR 50.0%
GCCCTTCTCTCAACAACCTT+TGG - tig0024288:1768-1787 None:intergenic 50.0%
GAAAGAGCTACGATGGCCCA+TGG + tig0024288:402-421 Msa1415800:CDS 55.0%
GCCTAGGTACCTGTCTGACT+TGG + tig0024288:1600-1619 Msa1415800:three_prime_UTR 55.0%
GAAAGAGCTACGATGGCCCA+TGG + tig0024288:402-421 Msa1415800:CDS 55.0%
GCCTAGGTACCTGTCTGACT+TGG + tig0024288:1600-1619 Msa1415800:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
tig0024288 gene 401 2005 401 ID=Msa1415800;Name=Msa1415800
tig0024288 mRNA 401 2005 401 ID=Msa1415800-mRNA-1;Parent=Msa1415800;Name=Msa1415800-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|4|426|130
tig0024288 exon 401 520 401 ID=Msa1415800-mRNA-1:exon:22619;Parent=Msa1415800-mRNA-1
tig0024288 exon 609 663 609 ID=Msa1415800-mRNA-1:exon:22620;Parent=Msa1415800-mRNA-1
tig0024288 exon 1228 1327 1228 ID=Msa1415800-mRNA-1:exon:22621;Parent=Msa1415800-mRNA-1
tig0024288 exon 1462 2005 1462 ID=Msa1415800-mRNA-1:exon:22622;Parent=Msa1415800-mRNA-1
tig0024288 CDS 401 520 401 ID=Msa1415800-mRNA-1:cds;Parent=Msa1415800-mRNA-1
tig0024288 CDS 609 663 609 ID=Msa1415800-mRNA-1:cds;Parent=Msa1415800-mRNA-1
tig0024288 CDS 1228 1327 1228 ID=Msa1415800-mRNA-1:cds;Parent=Msa1415800-mRNA-1
tig0024288 CDS 1462 1579 1462 ID=Msa1415800-mRNA-1:cds;Parent=Msa1415800-mRNA-1
tig0024288 three_prime_UTR 1580 2005 1580 ID=Msa1415800-mRNA-1:three_prime_utr;Parent=Msa1415800-mRNA-1
Gene Sequence

>Msa1415800

GGAAAGAGCTACGATGGCCCATGGCTGCTGTTTATTTCCGAGAGTGGTCATGGAAAACGGGTTCCTTTAAGCCGTTTCCGAATGTCTTCTTTGAATCGAGTTGGTCTGATTGGATACAAGTTTTCTGCGGAAGATCGCCTTGCTGCCGTATTTGTAGTAGGATTCACATTAGCAGAGGATGGTGAAAGTGATGAACAAGTGGTTCTTGTTAGCCAAAGTGGTACAGTCAACAGGATTAAAGTTCGGGATATTTCAATTCAATCCCGATTTGCTAGGGGAGTTTTTTTGATGCGACTTGATCATGCTGGAAAGATTCAGACTGCTTCCTTAATCTCAGCAACGAGTGCGAGCCTGAAGAAGAACTTGATATCGCCCAAGGCTAGTTGTAGATGA
Protein sequence

>Msa1415800

GKSYDGPWLLFISESGHGKRVPLSRFRMSSLNRVGLIGYKFSAEDRLAAVFVVGFTLAEDGESDEQVVLVSQSGTVNRIKVRDISIQSRFARGVFLMRLDHAGKIQTASLISATSASLKKNLISPKASCR*