AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1417040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417040 MtrunA17_Chr2g0308321 96.125 542 18 2 1 542 1 539 0.0 1061
Msa1417040 MtrunA17_Chr4g0061651 61.368 497 150 11 51 542 11 470 0.0 523
Msa1417040 MtrunA17_Chr5g0420051 40.162 371 189 11 1 355 1 354 2.86e-63 214
Msa1417040 MtrunA17_Chr3g0132091 34.204 383 178 15 125 492 18 341 9.34e-40 148
Msa1417040 MtrunA17_Chr8g0386271 58.462 130 49 2 125 249 45 174 5.36e-38 144
Msa1417040 MtrunA17_Chr7g0224821 38.168 262 113 6 169 423 72 291 1.46e-35 137
Msa1417040 MtrunA17_Chr7g0258471 58.929 112 44 2 165 274 133 244 1.11e-33 132
Msa1417040 MtrunA17_Chr7g0231911 60.377 106 36 2 168 267 143 248 1.38e-33 130
Msa1417040 MtrunA17_Chr1g0162871 62.376 101 37 1 150 249 79 179 4.32e-33 135
Msa1417040 MtrunA17_Chr8g0334671 72.368 76 21 0 174 249 187 262 1.16e-32 127
Msa1417040 MtrunA17_Chr7g0270541 51.613 124 58 2 124 247 97 218 2.20e-32 132
Msa1417040 MtrunA17_Chr2g0302791 66.279 86 27 1 172 255 137 222 2.23e-31 129
Msa1417040 MtrunA17_Chr2g0282521 67.500 80 26 0 174 253 64 143 2.32e-31 118
Msa1417040 MtrunA17_Chr3g0122411 47.020 151 68 3 106 249 95 240 2.75e-31 126
Msa1417040 MtrunA17_Chr4g0046991 67.532 77 25 0 174 250 73 149 4.43e-31 118
Msa1417040 MtrunA17_Chr1g0194121 57.009 107 41 1 146 247 114 220 8.18e-31 128
Msa1417040 MtrunA17_Chr8g0360361 68.919 74 23 0 174 247 65 138 2.37e-29 114
Msa1417040 MtrunA17_Chr8g0377421 52.809 89 41 1 160 247 117 205 3.82e-25 105
Msa1417040 MtrunA17_Chr8g0377401 41.732 127 60 1 120 246 122 234 3.20e-23 99.4
Msa1417040 MtrunA17_Chr2g0286661 46.237 93 50 0 158 250 87 179 4.87e-21 97.4
Msa1417040 MtrunA17_Chr1g0175031 68.085 47 15 0 174 220 74 120 2.42e-15 73.2
Msa1417040 MtrunA17_Chr2g0317041 68.085 47 15 0 174 220 99 145 2.48e-15 73.6
Msa1417040 MtrunA17_Chr8g0377431 45.588 68 37 0 160 227 137 204 7.91e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417040 AT1G69170.1 45.872 218 105 3 73 290 35 239 1.50e-49 176
Msa1417040 AT5G50570.1 40.392 255 116 10 125 357 42 282 4.54e-38 144
Msa1417040 AT5G50570.2 40.392 255 116 10 125 357 42 282 4.54e-38 144
Msa1417040 AT5G50670.1 40.392 255 116 10 125 357 42 282 4.54e-38 144
Msa1417040 AT5G50670.2 40.392 255 116 10 125 357 42 282 4.54e-38 144
Msa1417040 AT2G42200.1 55.645 124 47 3 159 274 57 180 4.32e-34 133
Msa1417040 AT1G20980.1 66.667 90 30 0 158 247 104 193 1.98e-33 136
Msa1417040 AT3G57920.1 62.857 105 38 1 146 249 30 134 2.49e-33 130
Msa1417040 AT1G27370.6 52.459 122 49 3 157 274 155 271 2.70e-33 130
Msa1417040 AT1G27370.1 52.459 122 49 3 157 274 155 271 6.56e-33 130
Msa1417040 AT1G27370.5 52.459 122 49 3 157 274 155 271 6.56e-33 130
Msa1417040 AT1G27370.3 52.459 122 49 3 157 274 155 271 6.56e-33 130
Msa1417040 AT1G27370.2 52.459 122 49 3 157 274 155 271 6.56e-33 130
Msa1417040 AT1G27370.4 52.459 122 49 3 157 274 155 271 6.56e-33 130
Msa1417040 AT1G27370.7 52.459 122 49 3 157 274 155 271 6.56e-33 130
Msa1417040 AT5G43270.3 65.854 82 28 0 170 251 165 246 1.09e-32 130
Msa1417040 AT5G43270.1 65.854 82 28 0 170 251 165 246 1.09e-32 130
Msa1417040 AT5G43270.2 65.854 82 28 0 170 251 165 246 1.09e-32 130
Msa1417040 AT2G47070.1 59.813 107 34 2 144 247 79 179 1.43e-32 133
Msa1417040 AT1G02065.1 74.324 74 19 0 174 247 188 261 2.58e-32 127
Msa1417040 AT1G76580.3 63.265 98 30 1 158 249 92 189 2.51e-31 129
Msa1417040 AT1G76580.2 63.265 98 30 1 158 249 92 189 2.54e-31 129
Msa1417040 AT1G76580.1 63.265 98 30 1 158 249 92 189 5.81e-31 129
Msa1417040 AT3G60030.1 67.901 81 26 0 167 247 120 200 7.33e-31 128
Msa1417040 AT2G33810.1 67.568 74 24 0 174 247 54 127 7.53e-31 117
Msa1417040 AT1G53160.2 60.870 92 32 1 171 258 51 142 6.02e-30 116
Msa1417040 AT1G53160.1 60.870 92 32 1 171 258 51 142 6.02e-30 116
Msa1417040 AT3G15270.1 53.097 113 46 3 174 279 63 175 6.05e-30 116
Msa1417040 AT1G27360.2 56.731 104 40 2 171 274 172 270 6.17e-30 122
Msa1417040 AT1G27360.1 56.731 104 40 2 171 274 172 270 6.17e-30 122
Msa1417040 AT1G27360.3 56.731 104 40 2 171 274 172 270 6.17e-30 122
Msa1417040 AT1G27360.4 56.731 104 40 2 171 274 172 270 6.17e-30 122
Msa1417040 AT1G53160.3 60.870 92 32 1 171 258 51 142 9.56e-30 115
Msa1417040 AT5G18830.2 53.333 90 38 2 160 248 127 213 2.10e-21 99.0
Msa1417040 AT5G18830.1 53.333 90 38 2 160 248 127 213 2.16e-21 99.0
Msa1417040 AT5G18830.3 50.549 91 42 1 160 250 127 214 2.16e-21 99.0
Msa1417040 AT2G47070.2 76.000 50 12 0 198 247 1 50 2.80e-18 89.4
Msa1417040 AT1G02065.2 68.750 48 15 0 174 221 188 235 6.19e-17 81.3

Find 156 sgRNAs with CRISPR-Local

Find 306 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTCTTTCATTATTATTT+AGG 0.144422 tig0024432:-46788 Msa1417040:five_prime_UTR
AATGAATGTGAAAGCCATTT+TGG 0.169213 tig0024432:+46740 None:intergenic
TTAGATTACATTCACTAAAT+TGG 0.174095 tig0024432:+41897 None:intergenic
TTTCCTTTCCTTCAAGAAAA+TGG 0.189331 tig0024432:-43228 Msa1417040:CDS
TCGATTGGCTTGAAGAATTC+TGG 0.190530 tig0024432:+42995 None:intergenic
TTTATGCTAAGTTGAGATTT+TGG 0.196525 tig0024432:-46474 Msa1417040:five_prime_UTR
TGTATCAATGTGTGACATTC+AGG 0.241788 tig0024432:-41970 Msa1417040:three_prime_UTR
TGAGGATGTGCATTTATAAT+TGG 0.243639 tig0024432:+43288 None:intergenic
CCACAAGTTGGTATTCATTC+TGG 0.255651 tig0024432:-44918 Msa1417040:CDS
ACTATCCAGGTTTCATTTGC+TGG 0.261393 tig0024432:-45009 Msa1417040:intron
AGAACCAAAGGTTCAATCTT+AGG 0.265075 tig0024432:-46333 Msa1417040:CDS
AGCTTCAGGTCTTTAAATAC+TGG 0.310334 tig0024432:+41753 None:intergenic
ACCTATTGAGTTTGAATGCT+TGG 0.311089 tig0024432:+46071 None:intergenic
GGGAGGTTGCTTCAGTCATA+TGG 0.315390 tig0024432:-44888 Msa1417040:CDS
GCATCAGAGGCGAGCCTTGC+AGG 0.317553 tig0024432:-42680 Msa1417040:CDS
AAACATGACTCATGCTATAA+TGG 0.320615 tig0024432:+42926 None:intergenic
TTACTGTTTGATATTAATAT+AGG 0.332199 tig0024432:+42838 None:intergenic
TCTCTTTCTGAAAATGCATT+TGG 0.333291 tig0024432:+46117 None:intergenic
TATTGTTGTACGAAGGAAAC+AGG 0.337560 tig0024432:+43256 None:intergenic
ATTGCTTACTGCCAAGTATA+TGG 0.338603 tig0024432:-45886 Msa1417040:CDS
ATCCAAGGAGGCTCGAATTC+CGG 0.339302 tig0024432:-43150 Msa1417040:CDS
GCTTTCTACGACGCTCATTA+TGG 0.341005 tig0024432:+44940 None:intergenic
CCAAGTATATGGATGTAATA+AGG 0.348075 tig0024432:-45875 Msa1417040:CDS
CCAACCTAAGATTGAACCTT+TGG 0.348079 tig0024432:+46329 None:intergenic
TTGTCATAAACACTTCAATA+AGG 0.351623 tig0024432:-41944 Msa1417040:three_prime_UTR
CGATTGGCTTGAAGAATTCT+GGG 0.354670 tig0024432:+42996 None:intergenic
CCTATTGAGTTTGAATGCTT+GGG 0.355993 tig0024432:+46072 None:intergenic
GGAGGTTGCTTCAGTCATAT+GGG 0.364253 tig0024432:-44887 Msa1417040:CDS
CCCCATCTGCCATTTCGAAA+TGG 0.368112 tig0024432:+42806 None:intergenic
TCAGGAAAGAACTTAATATC+TGG 0.386887 tig0024432:-42145 Msa1417040:three_prime_UTR
CCTTATTACATCCATATACT+TGG 0.392117 tig0024432:+45875 None:intergenic
ATCGTGGTCCCCTTTGAAAA+TGG 0.392209 tig0024432:+45973 None:intergenic
TTGATCTTGTGATGGAGTCT+TGG 0.393634 tig0024432:-46407 Msa1417040:exon
CACTTCCTAAGGAGGTATCT+TGG 0.394888 tig0024432:+43112 None:intergenic
TGAGTTTGAATGCTTGGGCT+TGG 0.395759 tig0024432:+46077 None:intergenic
CCTGTGCAGCTTGTTGTGAC+TGG 0.401726 tig0024432:-42198 Msa1417040:CDS
CCCATCTGCCATTTCGAAAT+GGG 0.406149 tig0024432:+42807 None:intergenic
CAAAGGTTCAATCTTAGGTT+GGG 0.407849 tig0024432:-46328 Msa1417040:CDS
AGCTGTCGTAAACGTCTTGC+AGG 0.408574 tig0024432:-44963 Msa1417040:CDS
TGAAGCTGACCAATGGCGGC+TGG 0.414356 tig0024432:-42067 Msa1417040:three_prime_UTR
CCTAAAACCGGTTCCGCCTT+CGG 0.416837 tig0024432:+43326 None:intergenic
TTACATTACATTCTAGCTGT+TGG 0.416888 tig0024432:+42013 None:intergenic
TTGGAAGAAGTAGTGGGAAT+GGG 0.418540 tig0024432:+46855 None:intergenic
ATAAAAGCCGAAGGCGGAAC+CGG 0.423047 tig0024432:-43333 Msa1417040:CDS
GATTTGAAGCTTGGTAGATT+TGG 0.426241 tig0024432:-46021 Msa1417040:CDS
GATGGAACTCAAAGTGAAAC+AGG 0.427062 tig0024432:+46888 None:intergenic
ATCCGGAATTCGAGCCTCCT+TGG 0.439031 tig0024432:+43148 None:intergenic
GGTTATGCAGAAGAAGCTTC+AGG 0.439622 tig0024432:+41739 None:intergenic
TCACTCATTGATTTGAAGCT+TGG 0.440222 tig0024432:-46030 Msa1417040:CDS
TGACTTTATCAGTGTGTACT+TGG 0.441137 tig0024432:+46836 None:intergenic
TGACTGGAAATATTCGTATC+AGG 0.442828 tig0024432:-42182 Msa1417040:CDS
GATGATGATGATGTTGTTGT+TGG 0.449615 tig0024432:-46180 Msa1417040:CDS
ATCAGCTGTTCAAGGATTAT+CGG 0.450349 tig0024432:-43064 Msa1417040:CDS
GGTGATCATAGAGATGGAAT+TGG 0.457178 tig0024432:-46000 Msa1417040:CDS
ATCGAAGAGGGTGAGGTCTT+CGG 0.459977 tig0024432:-45923 Msa1417040:CDS
GGTATTCATTCTGGAAGAGC+TGG 0.460050 tig0024432:-44909 Msa1417040:CDS
TTATTCCAAGATACCTCCTT+AGG 0.463781 tig0024432:-43117 Msa1417040:CDS
CCAAAGGTTCAATCTTAGGT+TGG 0.470404 tig0024432:-46329 Msa1417040:CDS
TTTAGTGATATGTTCTCAAA+GGG 0.471354 tig0024432:+46662 None:intergenic
ATTGAAGTCTTCACTTCCTA+AGG 0.476688 tig0024432:+43101 None:intergenic
GAAACAGAATCAGCTGTTCA+AGG 0.477684 tig0024432:-43072 Msa1417040:CDS
AGAAATGTGGAAATAGTAAC+TGG 0.480981 tig0024432:-43365 Msa1417040:CDS
CCCAAGCATTCAAACTCAAT+AGG 0.481003 tig0024432:-46072 Msa1417040:CDS
AATGGTCGTGCCATCTTCAC+AGG 0.486457 tig0024432:+42741 None:intergenic
TATTGCGTCCCAGCCGCCAT+TGG 0.487032 tig0024432:+42058 None:intergenic
ATGAGTCATGTTTCTGAAAG+TGG 0.489233 tig0024432:-42916 Msa1417040:CDS
GACCGTCTTTCCTGTGAAGA+TGG 0.490853 tig0024432:-42751 Msa1417040:CDS
CTGAAAGTGGCGTCTCGGAT+AGG 0.491794 tig0024432:-42903 Msa1417040:CDS
TGAAATCGCCCATTTCGAAA+TGG 0.493806 tig0024432:-42815 Msa1417040:CDS
TCAGCTGTTCAAGGATTATC+GGG 0.494261 tig0024432:-43063 Msa1417040:CDS
GCATGGGAATTCCTGTCGAT+TGG 0.495766 tig0024432:+42980 None:intergenic
CTGATGTTAGCATGGTTCCT+TGG 0.502694 tig0024432:+43436 None:intergenic
ACTTCTAAAGTAATAGTGAA+TGG 0.503268 tig0024432:-45793 Msa1417040:CDS
CCTCACCGGGGAGTATCTCT+TGG 0.503402 tig0024432:+43400 None:intergenic
CTTGGAAGAAGTAGTGGGAA+TGG 0.505481 tig0024432:+46854 None:intergenic
CAGGATAAGAACTTAGTGTC+AGG 0.506236 tig0024432:-42163 Msa1417040:exon
AATGGTGCTACATCTGCTAC+TGG 0.508879 tig0024432:-43210 Msa1417040:CDS
ATAATTGGAATAGAAGAAAG+AGG 0.509513 tig0024432:+43303 None:intergenic
ATGATTCTTCCTGCTCTCTA+CGG 0.510890 tig0024432:-42645 Msa1417040:CDS
TCCGTCTGAGTTAAATCATA+CGG 0.511466 tig0024432:-42878 Msa1417040:CDS
CCCATTTCGAAATGGCAGAT+GGG 0.511484 tig0024432:-42807 Msa1417040:CDS
GAAACATACCCTTTATCTTG+AGG 0.518442 tig0024432:+46375 None:intergenic
TTACGTAATCCGTAGAGAGC+AGG 0.520716 tig0024432:+42636 None:intergenic
GGCAGTAAGCAATCTGAGAA+TGG 0.521246 tig0024432:+45896 None:intergenic
GAAAATGACAACAATAATCA+AGG 0.521619 tig0024432:-46264 Msa1417040:CDS
TTGCTGGCTGAATTTGATGA+TGG 0.522089 tig0024432:-44993 Msa1417040:CDS
GTTTAGTGATATGTTCTCAA+AGG 0.522350 tig0024432:+46661 None:intergenic
AGAAGAAAGAGGCCTAAAAC+CGG 0.523177 tig0024432:+43314 None:intergenic
TTGGAATAATGATTGTGATC+CGG 0.528558 tig0024432:+43131 None:intergenic
CTATAATGGCTATGGCTGCT+CGG 0.529046 tig0024432:+42940 None:intergenic
CGACAGTTGAAGCAAGTCAA+TGG 0.529505 tig0024432:+42723 None:intergenic
AATAACTAAATGACCAGGCA+TGG 0.532105 tig0024432:+42963 None:intergenic
CCAGTCACAACAAGCTGCAC+AGG 0.532430 tig0024432:+42198 None:intergenic
GCCCATTTCGAAATGGCAGA+TGG 0.532890 tig0024432:-42808 Msa1417040:CDS
TCGACAGGAATTCCCATGCC+TGG 0.536060 tig0024432:-42976 Msa1417040:CDS
CCATTTCGAAATGGCAGATG+GGG 0.536154 tig0024432:-42806 Msa1417040:CDS
TATTCTTGCAACAATTGAAG+TGG 0.542041 tig0024432:-46435 Msa1417040:five_prime_UTR
ATTCTTGCAACAATTGAAGT+GGG 0.542274 tig0024432:-46434 Msa1417040:five_prime_UTR
TCAGAAGATAAGACCTCACC+GGG 0.542752 tig0024432:+43387 None:intergenic
GAGGTTGCTTCAGTCATATG+GGG 0.543950 tig0024432:-44886 Msa1417040:CDS
TTCGAGCCTCCTTGGATAGG+AGG 0.548698 tig0024432:+43156 None:intergenic
GACTCATGCTATAATGGCTA+TGG 0.554681 tig0024432:+42932 None:intergenic
TGGGGTCTTTGATCTTGTGA+TGG 0.556099 tig0024432:-46415 Msa1417040:exon
CAACACCATCGAAGAGGGTG+AGG 0.557112 tig0024432:-45930 Msa1417040:CDS
CGTTGACAAAATCCGACCCC+TGG 0.557627 tig0024432:+42779 None:intergenic
AGATTTGGTGATCATAGAGA+TGG 0.560484 tig0024432:-46006 Msa1417040:CDS
GTATTCATTCTGGAAGAGCT+GGG 0.561734 tig0024432:-44908 Msa1417040:CDS
GAAGCTGACCAATGGCGGCT+GGG 0.561893 tig0024432:-42066 Msa1417040:three_prime_UTR
GAATTCGAGCCTCCTTGGAT+AGG 0.563220 tig0024432:+43153 None:intergenic
GAAGACCTCACCCTCTTCGA+TGG 0.563679 tig0024432:+45925 None:intergenic
TGTTTCTGAAAGTGGCGTCT+CGG 0.565635 tig0024432:-42908 Msa1417040:CDS
TGTGTACTTGGAAGAAGTAG+TGG 0.566096 tig0024432:+46848 None:intergenic
AATTGTTTATTGTTGTACGA+AGG 0.567910 tig0024432:+43249 None:intergenic
TCCGTATGATTTAACTCAGA+CGG 0.569364 tig0024432:+42877 None:intergenic
TTGGATATATGTAGATAGAG+AGG 0.569382 tig0024432:+42032 None:intergenic
AATTCTTCAAGCCAATCGAC+AGG 0.569495 tig0024432:-42991 Msa1417040:CDS
GAAGTCTTCACTTCCTAAGG+AGG 0.571303 tig0024432:+43104 None:intergenic
CTTACAGATAGTAGATTCCA+AGG 0.572899 tig0024432:-43453 Msa1417040:intron
CTCAGAAGATAAGACCTCAC+CGG 0.577587 tig0024432:+43386 None:intergenic
ATATGCCAAGAGATACTCCC+CGG 0.578163 tig0024432:-43405 Msa1417040:CDS
CTCGGAATAACTAAATGACC+AGG 0.578215 tig0024432:+42958 None:intergenic
GGAAACAGGGTTCTTGAATG+AGG 0.578570 tig0024432:+43270 None:intergenic
TGCCATCTTCACAGGAAAGA+CGG 0.587669 tig0024432:+42749 None:intergenic
AGTCAATATCCTCCTATCCA+AGG 0.597117 tig0024432:-43165 Msa1417040:CDS
AAAAGATGCTGATGTTAGCA+TGG 0.597201 tig0024432:+43428 None:intergenic
TTCAGCGTGTTGAGCATCAG+AGG 0.601537 tig0024432:-42693 Msa1417040:CDS
AGTTGAAGCAGAGAACTGAA+TGG 0.603922 tig0024432:+46696 None:intergenic
CGGCTCGTGAAGCTGACCAA+TGG 0.603959 tig0024432:-42074 Msa1417040:three_prime_UTR
CGTCGTAGAAAGCCACAAGT+TGG 0.607871 tig0024432:-44930 Msa1417040:CDS
TGGATATATGTAGATAGAGA+GGG 0.609573 tig0024432:+42033 None:intergenic
CCAGAATGAATACCAACTTG+TGG 0.611115 tig0024432:+44918 None:intergenic
TCTGAGTTAAATCATACGGA+TGG 0.611214 tig0024432:-42874 Msa1417040:CDS
ATAACTAAATGACCAGGCAT+GGG 0.613150 tig0024432:+42964 None:intergenic
CTCGTGAAGCTGACCAATGG+CGG 0.613637 tig0024432:-42071 Msa1417040:three_prime_UTR
GTCTTATCTTCTGAGAAATG+TGG 0.623862 tig0024432:-43378 Msa1417040:CDS
ATCGTCAACACCATCGAAGA+GGG 0.624868 tig0024432:-45935 Msa1417040:CDS
GTGTACTTGGAAGAAGTAGT+GGG 0.629351 tig0024432:+46849 None:intergenic
GTTGTAAAGATTATCACAAG+AGG 0.629804 tig0024432:-45843 Msa1417040:CDS
TTCTTGCAACAATTGAAGTG+GGG 0.636712 tig0024432:-46433 Msa1417040:five_prime_UTR
ACACTTGGTAGTAGAACCAA+AGG 0.641812 tig0024432:-46345 Msa1417040:CDS
TATGTTTCAAATGACACACT+TGG 0.649632 tig0024432:-46360 Msa1417040:CDS
GATCGTCAACACCATCGAAG+AGG 0.650478 tig0024432:-45936 Msa1417040:CDS
TGCAGACCTATAAAAGCCGA+AGG 0.654610 tig0024432:-43342 Msa1417040:CDS
GGATTATCGGGAGTATCAGA+CGG 0.658305 tig0024432:-43051 Msa1417040:CDS
ATTGTTGTACGAAGGAAACA+GGG 0.661617 tig0024432:+43257 None:intergenic
TGGTGTTGACGATCCAGACG+AGG 0.673725 tig0024432:+45945 None:intergenic
CCAAGAGATACTCCCCGGTG+AGG 0.681967 tig0024432:-43400 Msa1417040:CDS
TGAAGCAGAGAACTGAATGG+AGG 0.683282 tig0024432:+46699 None:intergenic
GGATGTAATAAGGATTTGAG+TGG 0.689862 tig0024432:-45865 Msa1417040:CDS
TCCAGACGAGGACAAAATCG+TGG 0.694231 tig0024432:+45957 None:intergenic
CAATATCCTCCTATCCAAGG+AGG 0.696180 tig0024432:-43162 Msa1417040:CDS
TTCATTCTGGAAGAGCTGGG+AGG 0.705305 tig0024432:-44905 Msa1417040:CDS
AGACCTATAAAAGCCGAAGG+CGG 0.722914 tig0024432:-43339 Msa1417040:CDS
AGGTTGCTTCAGTCATATGG+GGG 0.727655 tig0024432:-44885 Msa1417040:intron
TTATCGGGAGTATCAGACGG+TGG 0.745082 tig0024432:-43048 Msa1417040:CDS
CAGAAGATAAGACCTCACCG+GGG 0.863209 tig0024432:+43388 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCTCTTTCATTATTATTT+AGG - tig0024432:41858-41877 Msa1417040:three_prime_UTR 15.0%
!! TTAAAAAAAATATGTCAGAA+GGG + tig0024432:42033-42052 None:intergenic 15.0%
!! ATTAAAAAAAATATGTCAGA+AGG + tig0024432:42034-42053 None:intergenic 15.0%
!! AAATAGGAATATTCATAAAA+TGG + tig0024432:43331-43350 None:intergenic 15.0%
!!! AAACAAAAAGATTCATTTTA+TGG - tig0024432:44645-44664 Msa1417040:intron 15.0%
!! GCTTTATAATATTAATAATC+AGG + tig0024432:45040-45059 None:intergenic 15.0%
!!! AAAAAAAAAGTCATTTTGAA+AGG + tig0024432:45062-45081 None:intergenic 15.0%
!! TTTAATATTCAAAACCATAT+TGG - tig0024432:45093-45112 Msa1417040:intron 15.0%
!! TTACTGTTTGATATTAATAT+AGG + tig0024432:45811-45830 None:intergenic 15.0%
!! GAAATGAAAATTTATTTATG+TGG - tig0024432:46325-46344 Msa1417040:CDS 15.0%
!!! TACTTTTCTTTTTTCCAAAA+TGG - tig0024432:41892-41911 Msa1417040:three_prime_UTR 20.0%
!! AGAAAACACAAAATTAGTTA+TGG - tig0024432:42990-43009 Msa1417040:CDS 20.0%
!!! TGTTTTTTCCATTTTAGAAA+AGG + tig0024432:43307-43326 None:intergenic 20.0%
!! ATCATAATCAACAGAAAAAT+AGG + tig0024432:43347-43366 None:intergenic 20.0%
!! AGTTGAAAATTAGAGTATAA+TGG - tig0024432:44081-44100 Msa1417040:intron 20.0%
!! TTAAGAACAAAGTGAAAATT+GGG + tig0024432:44196-44215 None:intergenic 20.0%
!! ATTAAGAACAAAGTGAAAAT+TGG + tig0024432:44197-44216 None:intergenic 20.0%
!! AAGAGAAAATTTGTAACAAA+TGG + tig0024432:44468-44487 None:intergenic 20.0%
!! TTAATAATCAGGAAAGTAAA+TGG + tig0024432:45029-45048 None:intergenic 20.0%
!! TAATTTGAAAACATGAAACA+TGG + tig0024432:46213-46232 None:intergenic 20.0%
!! TTAGATTACATTCACTAAAT+TGG + tig0024432:46752-46771 None:intergenic 20.0%
! TTTAGTGATATGTTCTCAAA+GGG + tig0024432:41987-42006 None:intergenic 25.0%
!!! TCTCAATCATCAATCTTTTT+TGG - tig0024432:42068-42087 Msa1417040:three_prime_UTR 25.0%
!!! TTTATGCTAAGTTGAGATTT+TGG - tig0024432:42172-42191 Msa1417040:CDS 25.0%
!!! TCATTTTCTATGTTTTGTTG+AGG + tig0024432:42370-42389 None:intergenic 25.0%
! GAAAATGACAACAATAATCA+AGG - tig0024432:42382-42401 Msa1417040:intron 25.0%
!!! ACTTCTAAAGTAATAGTGAA+TGG - tig0024432:42853-42872 Msa1417040:CDS 25.0%
!!! ATGTTTTGTTTCACATCTTT+TGG - tig0024432:42931-42950 Msa1417040:CDS 25.0%
!!! TTGCAAAAAATTTAAGTTGC+TGG - tig0024432:43056-43075 Msa1417040:CDS 25.0%
! AGTCCAAATTGATCAATATA+TGG - tig0024432:43228-43247 Msa1417040:CDS 25.0%
! AGACCATATATTGATCAATT+TGG + tig0024432:43234-43253 None:intergenic 25.0%
! AATTGATCAATATATGGTCT+TGG - tig0024432:43234-43253 Msa1417040:CDS 25.0%
!!! TTAATAGATTCAGTCGTTTT+CGG - tig0024432:43272-43291 Msa1417040:CDS 25.0%
!!! TACTACTTCCTTTTCTAAAA+TGG - tig0024432:43296-43315 Msa1417040:CDS 25.0%
!!! TTTTGACTTTTGCTTTACTA+AGG - tig0024432:43385-43404 Msa1417040:CDS 25.0%
! CAAGCAGATAAAGTAAAATT+TGG + tig0024432:43538-43557 None:intergenic 25.0%
!! AATTTTACTTTATCTGCTTG+AGG - tig0024432:43538-43557 Msa1417040:intron 25.0%
! AAATCCACTAAAAAGTAGTT+AGG + tig0024432:43842-43861 None:intergenic 25.0%
! TAAATATCGAGCTTGAAATT+TGG - tig0024432:43975-43994 Msa1417040:intron 25.0%
! AAATATCGAGCTTGAAATTT+GGG - tig0024432:43976-43995 Msa1417040:intron 25.0%
! AGATAATGATATCATCTACT+TGG - tig0024432:44130-44149 Msa1417040:intron 25.0%
!! TTTACAATTTTCCACAAAGA+TGG - tig0024432:44261-44280 Msa1417040:intron 25.0%
! AAACTGAATTACGAATACTT+TGG + tig0024432:44303-44322 None:intergenic 25.0%
!! TTGAATTCTGATTGATTCTA+TGG - tig0024432:44413-44432 Msa1417040:intron 25.0%
! AAATTATACTAGAGAGTTTG+AGG - tig0024432:44857-44876 Msa1417040:intron 25.0%
! ATTCAAAACCATATTGGTAA+AGG - tig0024432:45099-45118 Msa1417040:intron 25.0%
! TATGAAAACCTTTACCAATA+TGG + tig0024432:45110-45129 None:intergenic 25.0%
! ATAATTGGAATAGAAGAAAG+AGG + tig0024432:45346-45365 None:intergenic 25.0%
! AATTGTTTATTGTTGTACGA+AGG + tig0024432:45400-45419 None:intergenic 25.0%
!!! GAAGAATAAATCTTTGAAGT+CGG - tig0024432:46269-46288 Msa1417040:CDS 25.0%
! AAATGTTGTGAGTGATTTAA+TGG + tig0024432:46388-46407 None:intergenic 25.0%
! TTGTCATAAACACTTCAATA+AGG - tig0024432:46702-46721 Msa1417040:five_prime_UTR 25.0%
!!! ATTTTGATGAATGTTGATTC+TGG - tig0024432:46791-46810 Msa1417040:five_prime_UTR 25.0%
! AATGAATGTGAAAGCCATTT+TGG + tig0024432:41909-41928 None:intergenic 30.0%
GTTTAGTGATATGTTCTCAA+AGG + tig0024432:41988-42007 None:intergenic 30.0%
TATTCTTGCAACAATTGAAG+TGG - tig0024432:42211-42230 Msa1417040:CDS 30.0%
ATTCTTGCAACAATTGAAGT+GGG - tig0024432:42212-42231 Msa1417040:CDS 30.0%
!! AGTTTTGATCCTCAAGATAA+AGG - tig0024432:42262-42281 Msa1417040:intron 30.0%
!! GTTTTGATCCTCAAGATAAA+GGG - tig0024432:42263-42282 Msa1417040:intron 30.0%
TATGTTTCAAATGACACACT+TGG - tig0024432:42286-42305 Msa1417040:intron 30.0%
TTGAGGTAAAAAACTAGATG+GGG + tig0024432:42353-42372 None:intergenic 30.0%
GTTGAGGTAAAAAACTAGAT+GGG + tig0024432:42354-42373 None:intergenic 30.0%
TGTTGAGGTAAAAAACTAGA+TGG + tig0024432:42355-42374 None:intergenic 30.0%
! CAACAATAATCAAGGTTTTG+AGG - tig0024432:42390-42409 Msa1417040:intron 30.0%
!!! TAATCAAGGTTTTGAGGAAT+TGG - tig0024432:42396-42415 Msa1417040:intron 30.0%
!!! AATCAAGGTTTTGAGGAATT+GGG - tig0024432:42397-42416 Msa1417040:intron 30.0%
! CACTAGTAGTTCTTTTGTTA+TGG - tig0024432:42501-42520 Msa1417040:intron 30.0%
! TCTCTTTCTGAAAATGCATT+TGG + tig0024432:42532-42551 None:intergenic 30.0%
! AATTGGTACACCATTTTCAA+AGG - tig0024432:42663-42682 Msa1417040:CDS 30.0%
! ATTGGTACACCATTTTCAAA+GGG - tig0024432:42664-42683 Msa1417040:CDS 30.0%
CCTTATTACATCCATATACT+TGG + tig0024432:42774-42793 None:intergenic 30.0%
CCAAGTATATGGATGTAATA+AGG - tig0024432:42771-42790 Msa1417040:CDS 30.0%
GTTGTAAAGATTATCACAAG+AGG - tig0024432:42803-42822 Msa1417040:CDS 30.0%
! GATTTTGTCAACAATGTAGT+AGG - tig0024432:42887-42906 Msa1417040:CDS 30.0%
TTTGCAACATATGTTTGAGA+AGG + tig0024432:43043-43062 None:intergenic 30.0%
GAGGTTATATGTCAAGATTT+AGG + tig0024432:43126-43145 None:intergenic 30.0%
ATCTACATGACAAACAATTG+AGG + tig0024432:43148-43167 None:intergenic 30.0%
TACCAAATCTGCATTATTTG+TGG - tig0024432:43176-43195 Msa1417040:CDS 30.0%
ACCAAATCTGCATTATTTGT+GGG - tig0024432:43177-43196 Msa1417040:CDS 30.0%
!! TAATAGATTCAGTCGTTTTC+GGG - tig0024432:43273-43292 Msa1417040:CDS 30.0%
AATGAGTAACTTCACTTTAG+AGG + tig0024432:43506-43525 None:intergenic 30.0%
! TAATGTTTAGCTTACTATCC+AGG - tig0024432:43624-43643 Msa1417040:intron 30.0%
CACTAAAAAGTAGTTAGGAA+GGG + tig0024432:43837-43856 None:intergenic 30.0%
GATGATATCATTATCTGTGA+AGG + tig0024432:44126-44145 None:intergenic 30.0%
ACAAAAGTGGGAGAATAATT+CGG + tig0024432:44497-44516 None:intergenic 30.0%
!! AATTATTCTCCCACTTTTGT+TGG - tig0024432:44497-44516 Msa1417040:intron 30.0%
AGATGACAAATGATAAAGGA+TGG + tig0024432:44530-44549 None:intergenic 30.0%
CCAAAGATGACAAATGATAA+AGG + tig0024432:44534-44553 None:intergenic 30.0%
CCTTTATCATTTGTCATCTT+TGG - tig0024432:44531-44550 Msa1417040:intron 30.0%
!! GTGTAAAGCTAGCTTTAATT+TGG - tig0024432:44553-44572 Msa1417040:intron 30.0%
ATTTGGATTCTTTAGTCACA+TGG - tig0024432:44570-44589 Msa1417040:intron 30.0%
ATTGTTGAACAAGTCACTAA+TGG + tig0024432:44606-44625 None:intergenic 30.0%
! GACTTGTTCAACAATTACAA+TGG - tig0024432:44611-44630 Msa1417040:intron 30.0%
GGTTTAGTGACTATCATAAT+CGG - tig0024432:44666-44685 Msa1417040:intron 30.0%
! AATGCCAAATTTTCGTCAAT+GGG - tig0024432:44698-44717 Msa1417040:intron 30.0%
TCAAATAATCCAATGCAAAC+AGG - tig0024432:44968-44987 Msa1417040:CDS 30.0%
TAATTACTCAGAGACAAACA+TGG + tig0024432:45157-45176 None:intergenic 30.0%
GAGTAATTAACAAGATTGTG+TGG - tig0024432:45168-45187 Msa1417040:intron 30.0%
AGAAATGTGGAAATAGTAAC+TGG - tig0024432:45281-45300 Msa1417040:intron 30.0%
TGAGGATGTGCATTTATAAT+TGG + tig0024432:45361-45380 None:intergenic 30.0%
TTTCCTTTCCTTCAAGAAAA+TGG - tig0024432:45418-45437 Msa1417040:intron 30.0%
TTGGAATAATGATTGTGATC+CGG + tig0024432:45518-45537 None:intergenic 30.0%
AAACATGACTCATGCTATAA+TGG + tig0024432:45723-45742 None:intergenic 30.0%
TTACGTAAAATGCTTTCAAC+AGG - tig0024432:46025-46044 Msa1417040:CDS 30.0%
! GAAGTTCTTTTCTGCAAAAA+TGG + tig0024432:46426-46445 None:intergenic 30.0%
TCAGGAAAGAACTTAATATC+TGG - tig0024432:46501-46520 Msa1417040:five_prime_UTR 30.0%
!! TAGTTTTACTTGTGCTGAAT+CGG - tig0024432:46552-46571 Msa1417040:five_prime_UTR 30.0%
TGGATATATGTAGATAGAGA+GGG + tig0024432:46616-46635 None:intergenic 30.0%
TTGGATATATGTAGATAGAG+AGG + tig0024432:46617-46636 None:intergenic 30.0%
! TTACATTACATTCTAGCTGT+TGG + tig0024432:46636-46655 None:intergenic 30.0%
TGACTTTATCAGTGTGTACT+TGG + tig0024432:41813-41832 None:intergenic 35.0%
!! GAGATTTTGGTACTGTTTTG+AGG - tig0024432:42185-42204 Msa1417040:CDS 35.0%
TTCTTGCAACAATTGAAGTG+GGG - tig0024432:42213-42232 Msa1417040:CDS 35.0%
GAAACATACCCTTTATCTTG+AGG + tig0024432:42274-42293 None:intergenic 35.0%
AGAACCAAAGGTTCAATCTT+AGG - tig0024432:42313-42332 Msa1417040:intron 35.0%
CAAAGGTTCAATCTTAGGTT+GGG - tig0024432:42318-42337 Msa1417040:intron 35.0%
! TTTGAGGAATTGGGTTTTCA+TGG - tig0024432:42406-42425 Msa1417040:intron 35.0%
! ATTGGGTTTTCATGGTATGT+TGG - tig0024432:42414-42433 Msa1417040:intron 35.0%
! TTGGGTTTTCATGGTATGTT+GGG - tig0024432:42415-42434 Msa1417040:intron 35.0%
! GATGATGATGATGTTGTTGT+TGG - tig0024432:42466-42485 Msa1417040:intron 35.0%
CCTATTGAGTTTGAATGCTT+GGG + tig0024432:42577-42596 None:intergenic 35.0%
ACCTATTGAGTTTGAATGCT+TGG + tig0024432:42578-42597 None:intergenic 35.0%
!! TCACTCATTGATTTGAAGCT+TGG - tig0024432:42616-42635 Msa1417040:intron 35.0%
! GATTTGAAGCTTGGTAGATT+TGG - tig0024432:42625-42644 Msa1417040:intron 35.0%
!! AGATTTGGTGATCATAGAGA+TGG - tig0024432:42640-42659 Msa1417040:CDS 35.0%
! TTGGTACACCATTTTCAAAG+GGG - tig0024432:42665-42684 Msa1417040:CDS 35.0%
ATTGCTTACTGCCAAGTATA+TGG - tig0024432:42760-42779 Msa1417040:CDS 35.0%
GGATGTAATAAGGATTTGAG+TGG - tig0024432:42781-42800 Msa1417040:CDS 35.0%
TTGTCAACAATGTAGTAGGT+TGG - tig0024432:42891-42910 Msa1417040:CDS 35.0%
TACATGACAAACAATTGAGG+AGG + tig0024432:43145-43164 None:intergenic 35.0%
CCAAATCTGCATTATTTGTG+GGG - tig0024432:43178-43197 Msa1417040:CDS 35.0%
ATTATTTGTGGGGAAATGGT+TGG - tig0024432:43188-43207 Msa1417040:CDS 35.0%
! GACTTTTGCTTTACTAAGGT+TGG - tig0024432:43389-43408 Msa1417040:CDS 35.0%
!! GGATTTTAGTCACTTAAGAG+CGG - tig0024432:43410-43429 Msa1417040:CDS 35.0%
CATATGGGGGTAATATTCTA+TGG - tig0024432:43774-43793 Msa1417040:intron 35.0%
TAAAAAGTAGTTAGGAAGGG+TGG + tig0024432:43834-43853 None:intergenic 35.0%
CCACTAAAAAGTAGTTAGGA+AGG + tig0024432:43838-43857 None:intergenic 35.0%
!!! CCTTCCTAACTACTTTTTAG+TGG - tig0024432:43835-43854 Msa1417040:intron 35.0%
!! CAATGGGCTAATTAATTGCA+TGG + tig0024432:43888-43907 None:intergenic 35.0%
CATGCAATTAATTAGCCCAT+TGG - tig0024432:43886-43905 Msa1417040:intron 35.0%
AACATCTGATCTTGATCCAA+CGG + tig0024432:43915-43934 None:intergenic 35.0%
!!! ACATCTTTTTTCCCTTCATC+GGG + tig0024432:44385-44404 None:intergenic 35.0%
!!! CACATCTTTTTTCCCTTCAT+CGG + tig0024432:44386-44405 None:intergenic 35.0%
ATGGATATAGATCTACAGCA+TGG - tig0024432:44432-44451 Msa1417040:intron 35.0%
! GAATGCCAAATTTTCGTCAA+TGG - tig0024432:44697-44716 Msa1417040:intron 35.0%
TAATTAGCTGTTCGTCCAAA+TGG + tig0024432:44726-44745 None:intergenic 35.0%
CTTACAGATAGTAGATTCCA+AGG - tig0024432:45193-45212 Msa1417040:intron 35.0%
AAAAGATGCTGATGTTAGCA+TGG + tig0024432:45221-45240 None:intergenic 35.0%
! GTCTTATCTTCTGAGAAATG+TGG - tig0024432:45268-45287 Msa1417040:intron 35.0%
ATTGTTGTACGAAGGAAACA+GGG + tig0024432:45392-45411 None:intergenic 35.0%
TATTGTTGTACGAAGGAAAC+AGG + tig0024432:45393-45412 None:intergenic 35.0%
! ATGTAGCACCATTTTCTTGA+AGG + tig0024432:45429-45448 None:intergenic 35.0%
TTATTCCAAGATACCTCCTT+AGG - tig0024432:45529-45548 Msa1417040:intron 35.0%
ATTGAAGTCTTCACTTCCTA+AGG + tig0024432:45548-45567 None:intergenic 35.0%
ATCAGCTGTTCAAGGATTAT+CGG - tig0024432:45582-45601 Msa1417040:intron 35.0%
ATAACTAAATGACCAGGCAT+GGG + tig0024432:45685-45704 None:intergenic 35.0%
AATAACTAAATGACCAGGCA+TGG + tig0024432:45686-45705 None:intergenic 35.0%
! ATGAGTCATGTTTCTGAAAG+TGG - tig0024432:45730-45749 Msa1417040:intron 35.0%
TCCGTCTGAGTTAAATCATA+CGG - tig0024432:45768-45787 Msa1417040:CDS 35.0%
TCCGTATGATTTAACTCAGA+CGG + tig0024432:45772-45791 None:intergenic 35.0%
TCTGAGTTAAATCATACGGA+TGG - tig0024432:45772-45791 Msa1417040:CDS 35.0%
! TCACCAAATTTTCTCTACGT+CGG - tig0024432:46077-46096 Msa1417040:CDS 35.0%
AAATACACAGTAGTACGACA+TGG + tig0024432:46125-46144 None:intergenic 35.0%
TGACTGGAAATATTCGTATC+AGG - tig0024432:46464-46483 Msa1417040:five_prime_UTR 35.0%
TGTATCAATGTGTGACATTC+AGG - tig0024432:46676-46695 Msa1417040:five_prime_UTR 35.0%
AGCTTCAGGTCTTTAAATAC+TGG + tig0024432:46896-46915 None:intergenic 35.0%
GATGGAACTCAAAGTGAAAC+AGG + tig0024432:41761-41780 None:intergenic 40.0%
! TTGGAAGAAGTAGTGGGAAT+GGG + tig0024432:41794-41813 None:intergenic 40.0%
GTGTACTTGGAAGAAGTAGT+GGG + tig0024432:41800-41819 None:intergenic 40.0%
TGTGTACTTGGAAGAAGTAG+TGG + tig0024432:41801-41820 None:intergenic 40.0%
AGTTGAAGCAGAGAACTGAA+TGG + tig0024432:41953-41972 None:intergenic 40.0%
! TTGATCTTGTGATGGAGTCT+TGG - tig0024432:42239-42258 Msa1417040:intron 40.0%
! ACACTTGGTAGTAGAACCAA+AGG - tig0024432:42301-42320 Msa1417040:intron 40.0%
CCAACCTAAGATTGAACCTT+TGG + tig0024432:42320-42339 None:intergenic 40.0%
CCAAAGGTTCAATCTTAGGT+TGG - tig0024432:42317-42336 Msa1417040:intron 40.0%
CCCAAGCATTCAAACTCAAT+AGG - tig0024432:42574-42593 Msa1417040:intron 40.0%
GGTGATCATAGAGATGGAAT+TGG - tig0024432:42646-42665 Msa1417040:CDS 40.0%
CCCCACAAATAATGCAGATT+TGG + tig0024432:43181-43200 None:intergenic 40.0%
CTGCATTATTTGTGGGGAAA+TGG - tig0024432:43184-43203 Msa1417040:CDS 40.0%
ACTATCCAGGTTTCATTTGC+TGG - tig0024432:43637-43656 Msa1417040:intron 40.0%
! TTGCTGGCTGAATTTGATGA+TGG - tig0024432:43653-43672 Msa1417040:intron 40.0%
CCAGAATGAATACCAACTTG+TGG + tig0024432:43731-43750 None:intergenic 40.0%
!! CCACAAGTTGGTATTCATTC+TGG - tig0024432:43728-43747 Msa1417040:intron 40.0%
! GTATTCATTCTGGAAGAGCT+GGG - tig0024432:43738-43757 Msa1417040:intron 40.0%
GCAATTAATTAGCCCATTGG+TGG - tig0024432:43889-43908 Msa1417040:intron 40.0%
CTTCAGCAAATATATGCACG+TGG + tig0024432:44063-44082 None:intergenic 40.0%
TGATAGGCATTCCATCTTTG+TGG + tig0024432:44275-44294 None:intergenic 40.0%
AAGATGGAATGCCTATCATC+TGG - tig0024432:44277-44296 Msa1417040:intron 40.0%
GAATACTTTGGCCAGATGAT+AGG + tig0024432:44291-44310 None:intergenic 40.0%
!!! CATCTTTTTTCCCTTCATCG+GGG + tig0024432:44384-44403 None:intergenic 40.0%
GGTGCAAAACCAACAAAAGT+GGG + tig0024432:44509-44528 None:intergenic 40.0%
TGGTGCAAAACCAACAAAAG+TGG + tig0024432:44510-44529 None:intergenic 40.0%
TGGATTCTTTAGTCACATGG+TGG - tig0024432:44573-44592 Msa1417040:intron 40.0%
GGTTCCCATTGACGAAAATT+TGG + tig0024432:44705-44724 None:intergenic 40.0%
TTTCGTCAATGGGAACCATT+TGG - tig0024432:44708-44727 Msa1417040:intron 40.0%
AGACTGCTTGGAATAGCTTA+TGG + tig0024432:44921-44940 None:intergenic 40.0%
AGAAGAAAGAGGCCTAAAAC+CGG + tig0024432:45335-45354 None:intergenic 40.0%
! GCACCATTTTCTTGAAGGAA+AGG + tig0024432:45424-45443 None:intergenic 40.0%
AGTCAATATCCTCCTATCCA+AGG - tig0024432:45481-45500 Msa1417040:intron 40.0%
GAAACAGAATCAGCTGTTCA+AGG - tig0024432:45574-45593 Msa1417040:intron 40.0%
TCAGCTGTTCAAGGATTATC+GGG - tig0024432:45583-45602 Msa1417040:intron 40.0%
!! CGATTGGCTTGAAGAATTCT+GGG + tig0024432:45653-45672 None:intergenic 40.0%
!! TCGATTGGCTTGAAGAATTC+TGG + tig0024432:45654-45673 None:intergenic 40.0%
AATTCTTCAAGCCAATCGAC+AGG - tig0024432:45655-45674 Msa1417040:intron 40.0%
CTCGGAATAACTAAATGACC+AGG + tig0024432:45691-45710 None:intergenic 40.0%
GACTCATGCTATAATGGCTA+TGG + tig0024432:45717-45736 None:intergenic 40.0%
TGAAATCGCCCATTTCGAAA+TGG - tig0024432:45831-45850 Msa1417040:CDS 40.0%
ATGATTCTTCCTGCTCTCTA+CGG - tig0024432:46001-46020 Msa1417040:CDS 40.0%
TAGCCGACGTAGAGAAAATT+TGG + tig0024432:46083-46102 None:intergenic 40.0%
CAGGATAAGAACTTAGTGTC+AGG - tig0024432:46483-46502 Msa1417040:five_prime_UTR 40.0%
!! GTAGTGGGAATGGGTTTTGA+GGG + tig0024432:41785-41804 None:intergenic 45.0%
!! AGTAGTGGGAATGGGTTTTG+AGG + tig0024432:41786-41805 None:intergenic 45.0%
! CTTGGAAGAAGTAGTGGGAA+TGG + tig0024432:41795-41814 None:intergenic 45.0%
TGAAGCAGAGAACTGAATGG+AGG + tig0024432:41950-41969 None:intergenic 45.0%
TGGGGTCTTTGATCTTGTGA+TGG - tig0024432:42231-42250 Msa1417040:intron 45.0%
TGAGTTTGAATGCTTGGGCT+TGG + tig0024432:42572-42591 None:intergenic 45.0%
! ATCGTGGTCCCCTTTGAAAA+TGG + tig0024432:42676-42695 None:intergenic 45.0%
ATCGTCAACACCATCGAAGA+GGG - tig0024432:42711-42730 Msa1417040:CDS 45.0%
GGCAGTAAGCAATCTGAGAA+TGG + tig0024432:42753-42772 None:intergenic 45.0%
TTCAGCCAGCAAATGAAACC+TGG + tig0024432:43645-43664 None:intergenic 45.0%
GCTTTCTACGACGCTCATTA+TGG + tig0024432:43709-43728 None:intergenic 45.0%
! GGTATTCATTCTGGAAGAGC+TGG - tig0024432:43737-43756 Msa1417040:intron 45.0%
GGAGGTTGCTTCAGTCATAT+GGG - tig0024432:43759-43778 Msa1417040:intron 45.0%
GAGGTTGCTTCAGTCATATG+GGG - tig0024432:43760-43779 Msa1417040:intron 45.0%
AGGTTGCTTCAGTCATATGG+GGG - tig0024432:43761-43780 Msa1417040:intron 45.0%
GGAAGGGTGGAAAATTGAGA+AGG + tig0024432:43821-43840 None:intergenic 45.0%
! GACCACCTATGTATTGATCG+CGG - tig0024432:44019-44038 Msa1417040:intron 45.0%
TTCCGCGATCAATACATAGG+TGG + tig0024432:44024-44043 None:intergenic 45.0%
GCATTCCGCGATCAATACAT+AGG + tig0024432:44027-44046 None:intergenic 45.0%
TTAGAACCTGTGAGACTGCT+TGG + tig0024432:44933-44952 None:intergenic 45.0%
GCTTTACAGCCTGTTTGCAT+TGG + tig0024432:44980-44999 None:intergenic 45.0%
CTGATGTTAGCATGGTTCCT+TGG + tig0024432:45213-45232 None:intergenic 45.0%
ATATGCCAAGAGATACTCCC+CGG - tig0024432:45241-45260 Msa1417040:intron 45.0%
TCAGAAGATAAGACCTCACC+GGG + tig0024432:45262-45281 None:intergenic 45.0%
CTCAGAAGATAAGACCTCAC+CGG + tig0024432:45263-45282 None:intergenic 45.0%
TGCAGACCTATAAAAGCCGA+AGG - tig0024432:45304-45323 Msa1417040:intron 45.0%
AGACCTATAAAAGCCGAAGG+CGG - tig0024432:45307-45326 Msa1417040:intron 45.0%
GGAAACAGGGTTCTTGAATG+AGG + tig0024432:45379-45398 None:intergenic 45.0%
!! AATGGTGCTACATCTGCTAC+TGG - tig0024432:45436-45455 Msa1417040:intron 45.0%
CAATATCCTCCTATCCAAGG+AGG - tig0024432:45484-45503 Msa1417040:intron 45.0%
!! CACTTCCTAAGGAGGTATCT+TGG + tig0024432:45537-45556 None:intergenic 45.0%
GAAGTCTTCACTTCCTAAGG+AGG + tig0024432:45545-45564 None:intergenic 45.0%
!! GGATTATCGGGAGTATCAGA+CGG - tig0024432:45595-45614 Msa1417040:intron 45.0%
CTATAATGGCTATGGCTGCT+CGG + tig0024432:45709-45728 None:intergenic 45.0%
! TGTTTCTGAAAGTGGCGTCT+CGG - tig0024432:45738-45757 Msa1417040:intron 45.0%
! CCCATCTGCCATTTCGAAAT+GGG + tig0024432:45842-45861 None:intergenic 45.0%
CCCATTTCGAAATGGCAGAT+GGG - tig0024432:45839-45858 Msa1417040:CDS 45.0%
CCATTTCGAAATGGCAGATG+GGG - tig0024432:45840-45859 Msa1417040:CDS 45.0%
TGCCATCTTCACAGGAAAGA+CGG + tig0024432:45900-45919 None:intergenic 45.0%
CGACAGTTGAAGCAAGTCAA+TGG + tig0024432:45926-45945 None:intergenic 45.0%
! ATTTTCTCGCTTCACCTGCA+AGG + tig0024432:45983-46002 None:intergenic 45.0%
TTACGTAATCCGTAGAGAGC+AGG + tig0024432:46013-46032 None:intergenic 45.0%
!! TTTGAATATTAAATAAAAAA+TGG + tig0024432:45086-45105 None:intergenic 5.0%
!!! GGAATGGGTTTTGAGGGTGA+TGG + tig0024432:41779-41798 None:intergenic 50.0%
! ACCACGATTTTGTCCTCGTC+TGG - tig0024432:42688-42707 Msa1417040:CDS 50.0%
TCCAGACGAGGACAAAATCG+TGG + tig0024432:42692-42711 None:intergenic 50.0%
GATCGTCAACACCATCGAAG+AGG - tig0024432:42710-42729 Msa1417040:CDS 50.0%
ATCGAAGAGGGTGAGGTCTT+CGG - tig0024432:42723-42742 Msa1417040:CDS 50.0%
AGCTGTCGTAAACGTCTTGC+AGG - tig0024432:43683-43702 Msa1417040:intron 50.0%
CGTCGTAGAAAGCCACAAGT+TGG - tig0024432:43716-43735 Msa1417040:intron 50.0%
! TTCATTCTGGAAGAGCTGGG+AGG - tig0024432:43741-43760 Msa1417040:intron 50.0%
GGGAGGTTGCTTCAGTCATA+TGG - tig0024432:43758-43777 Msa1417040:intron 50.0%
TTGATCCAACGGCCACCAAT+GGG + tig0024432:43904-43923 None:intergenic 50.0%
! TGTATTGATCGCGGAATGCG+TGG - tig0024432:44028-44047 Msa1417040:intron 50.0%
! GTATTGATCGCGGAATGCGT+GGG - tig0024432:44029-44048 Msa1417040:intron 50.0%
ACGTGTTCTTACCCCGATGA+AGG - tig0024432:44370-44389 Msa1417040:intron 50.0%
CGTGTTCTTACCCCGATGAA+GGG - tig0024432:44371-44390 Msa1417040:intron 50.0%
GCTATTCCAAGCAGTCTCAC+AGG - tig0024432:44924-44943 Msa1417040:CDS 50.0%
CAGAAGATAAGACCTCACCG+GGG + tig0024432:45261-45280 None:intergenic 50.0%
! GTTCCGCCTTCGGCTTTTAT+AGG + tig0024432:45313-45332 None:intergenic 50.0%
ATAAAAGCCGAAGGCGGAAC+CGG - tig0024432:45313-45332 Msa1417040:intron 50.0%
GAATTCGAGCCTCCTTGGAT+AGG + tig0024432:45496-45515 None:intergenic 50.0%
ATCCAAGGAGGCTCGAATTC+CGG - tig0024432:45496-45515 Msa1417040:intron 50.0%
!! TTATCGGGAGTATCAGACGG+TGG - tig0024432:45598-45617 Msa1417040:intron 50.0%
GCATGGGAATTCCTGTCGAT+TGG + tig0024432:45669-45688 None:intergenic 50.0%
GCCCATTTCGAAATGGCAGA+TGG - tig0024432:45838-45857 Msa1417040:CDS 50.0%
! CCCCATCTGCCATTTCGAAA+TGG + tig0024432:45843-45862 None:intergenic 50.0%
! AATGGCAGATGGGGTTTTCC+AGG - tig0024432:45849-45868 Msa1417040:CDS 50.0%
! ATGGCAGATGGGGTTTTCCA+GGG - tig0024432:45850-45869 Msa1417040:CDS 50.0%
GACCGTCTTTCCTGTGAAGA+TGG - tig0024432:45895-45914 Msa1417040:CDS 50.0%
AATGGTCGTGCCATCTTCAC+AGG + tig0024432:45908-45927 None:intergenic 50.0%
TTCAGCGTGTTGAGCATCAG+AGG - tig0024432:45953-45972 Msa1417040:CDS 50.0%
!! TGGTGTTGACGATCCAGACG+AGG + tig0024432:42704-42723 None:intergenic 55.0%
CAACACCATCGAAGAGGGTG+AGG - tig0024432:42716-42735 Msa1417040:CDS 55.0%
GAAGACCTCACCCTCTTCGA+TGG + tig0024432:42724-42743 None:intergenic 55.0%
! ATTAGCCCATTGGTGGCCGT+TGG - tig0024432:43896-43915 Msa1417040:intron 55.0%
CTTGATCCAACGGCCACCAA+TGG + tig0024432:43905-43924 None:intergenic 55.0%
! CCTAAAACCGGTTCCGCCTT+CGG + tig0024432:45323-45342 None:intergenic 55.0%
TTCGAGCCTCCTTGGATAGG+AGG + tig0024432:45493-45512 None:intergenic 55.0%
ATCCGGAATTCGAGCCTCCT+TGG + tig0024432:45501-45520 None:intergenic 55.0%
TCGACAGGAATTCCCATGCC+TGG - tig0024432:45670-45689 Msa1417040:intron 55.0%
CTGAAAGTGGCGTCTCGGAT+AGG - tig0024432:45743-45762 Msa1417040:intron 55.0%
! TGGCAGATGGGGTTTTCCAG+GGG - tig0024432:45851-45870 Msa1417040:CDS 55.0%
! AGATGGGGTTTTCCAGGGGT+CGG - tig0024432:45855-45874 Msa1417040:CDS 55.0%
CGTTGACAAAATCCGACCCC+TGG + tig0024432:45870-45889 None:intergenic 55.0%
CCAGTCACAACAAGCTGCAC+AGG + tig0024432:46451-46470 None:intergenic 55.0%
!! CCTGTGCAGCTTGTTGTGAC+TGG - tig0024432:46448-46467 Msa1417040:five_prime_UTR 55.0%
CTCGTGAAGCTGACCAATGG+CGG - tig0024432:46575-46594 Msa1417040:five_prime_UTR 55.0%
!! CCTCACCGGGGAGTATCTCT+TGG + tig0024432:45249-45268 None:intergenic 60.0%
CCAAGAGATACTCCCCGGTG+AGG - tig0024432:45246-45265 Msa1417040:intron 60.0%
!! CCGAAGGCGGAACCGGTTTT+AGG - tig0024432:45320-45339 Msa1417040:intron 60.0%
CGGCTCGTGAAGCTGACCAA+TGG - tig0024432:46572-46591 Msa1417040:five_prime_UTR 60.0%
TGAAGCTGACCAATGGCGGC+TGG - tig0024432:46579-46598 Msa1417040:five_prime_UTR 60.0%
GAAGCTGACCAATGGCGGCT+GGG - tig0024432:46580-46599 Msa1417040:five_prime_UTR 60.0%
TATTGCGTCCCAGCCGCCAT+TGG + tig0024432:46591-46610 None:intergenic 60.0%
GCATCAGAGGCGAGCCTTGC+AGG - tig0024432:45966-45985 Msa1417040:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0024432 gene 41745 46923 41745 ID=Msa1417040;Name=Msa1417040
tig0024432 mRNA 41745 46923 41745 ID=Msa1417040-mRNA-1;Parent=Msa1417040;Name=Msa1417040-mRNA-1;_AED=0.01;_eAED=0.01;_QI=505|0.66|0.75|1|1|1|4|425|562
tig0024432 exon 41745 42233 41745 ID=Msa1417040-mRNA-1:exon:23158;Parent=Msa1417040-mRNA-1
tig0024432 exon 42640 43468 42640 ID=Msa1417040-mRNA-1:exon:23157;Parent=Msa1417040-mRNA-1
tig0024432 exon 44886 45022 44886 ID=Msa1417040-mRNA-1:exon:23156;Parent=Msa1417040-mRNA-1
tig0024432 exon 45760 46923 45760 ID=Msa1417040-mRNA-1:exon:23155;Parent=Msa1417040-mRNA-1
tig0024432 five_prime_UTR 46419 46923 46419 ID=Msa1417040-mRNA-1:five_prime_utr;Parent=Msa1417040-mRNA-1
tig0024432 CDS 45760 46418 45760 ID=Msa1417040-mRNA-1:cds;Parent=Msa1417040-mRNA-1
tig0024432 CDS 44886 45022 44886 ID=Msa1417040-mRNA-1:cds;Parent=Msa1417040-mRNA-1
tig0024432 CDS 42640 43468 42640 ID=Msa1417040-mRNA-1:cds;Parent=Msa1417040-mRNA-1
tig0024432 CDS 42170 42233 42170 ID=Msa1417040-mRNA-1:cds;Parent=Msa1417040-mRNA-1
tig0024432 three_prime_UTR 41745 42169 41745 ID=Msa1417040-mRNA-1:three_prime_utr;Parent=Msa1417040-mRNA-1
Gene Sequence

>Msa1417040

ATGGAGTCTTGGAGTTTTGATCCTCAAGATAAAGGGTATGTTTCAAATGACACACTTGGTAGTAGAACCAAAGGTTCAATCTTAGGTTGGGATTTGAAAACCCCATCTAGTTTTTTACCTCAACAAAACATAGAAAATGACAACAATAATCAAGGTTTTGAGGAATTGGGTTTTCATGGTATGTTGGGAAAACAATTGTCTAATGTTGTTGATGATGATGATGATGATGTTGTTGTTGGTAGCAAAATTGTCACTAGTAGTTCTTTTGTTATGGCTGCACCAAATGCATTTTCAGAAAGAGAACAACAACACTTCAATTCCAAGCCCAAGCATTCAAACTCAATAGGTGACACTAATAGTTCTAATTCACTCATTGATTTGAAGCTTGGTAGATTTGGTGATCATAGAGATGGAATTGGTACACCATTTTCAAAGGGGACCACGATTTTGTCCTCGTCTGGATCGTCAACACCATCGAAGAGGGTGAGGTCTTCGGCAGTCCATTCTCAGATTGCTTACTGCCAAGTATATGGATGTAATAAGGATTTGAGTGGTTGTAAAGATTATCACAAGAGGCATAAAGTTTGTGAAGTTCATTCAAAGACTTCTAAAGTAATAGTGAATGGAATTGAACAAAGATTTTGTCAACAATGTAGTAGGTTTCATTTGCTGGCTGAATTTGATGATGGTAAGCGTAGCTGTCGTAAACGTCTTGCAGGCCATAATGAGCGTCGTAGAAAGCCACAAGTTGGTATTCATTCTGGAAGAGCTGGGAGGTTGCTTCAGTCATATGGGGATAGTAGATTCCAAGGAACCATGCTAACATCAGCATCTTTTATATGCCAAGAGATACTCCCCGGTGAGGTCTTATCTTCTGAGAAATGTGGAAATAGTAACTGGTGCAGACCTATAAAAGCCGAAGGCGGAACCGGTTTTAGGCCTCTTTCTTCTATTCCAATTATAAATGCACATCCTCATTCAAGAACCCTGTTTCCTTCGTACAACAATAAACAATTTCCTTTCCTTCAAGAAAATGGTGCTACATCTGCTACTGGAAGCATCTTCTGTGAAAACAGTAGTCAATATCCTCCTATCCAAGGAGGCTCGAATTCCGGATCACAATCATTATTCCAAGATACCTCCTTAGGAAGTGAAGACTTCAATGTTTTTGAAACAGAATCAGCTGTTCAAGGATTATCGGGAGTATCAGACGGTGGTTGTGCTCTCTCTCTTCTGTCATCTCAATCCCAGAATTCTTCAAGCCAATCGACAGGAATTCCCATGCCTGGTCATTTAGTTATTCCGAGCAGCCATAGCCATTATAGCATGAGTCATGTTTCTGAAAGTGGCGTCTCGGATAGGTTTCCGTCTGAGTTAAATCATACGGATGGAAGTCATCTTAGTCCTATATTAATATCAAACAGTAATGAAATCGCCCATTTCGAAATGGCAGATGGGGTTTTCCAGGGGTCGGATTTTGTCAACGTGAAAGACCGTCTTTCCTGTGAAGATGGCACGACCATTGACTTGCTTCAACTGTCGTCACAACTTCAGCGTGTTGAGCATCAGAGGCGAGCCTTGCAGGTGAAGCGAGAAAATGATTCTTCCTGCTCTCTACGGATTACAAAAGAACTTCTGCCTGTGCAGCTTGTTGTGACTGGAAATATTCGTATCAGGATAAGAACTTAG
Protein sequence

>Msa1417040

MESWSFDPQDKGYVSNDTLGSRTKGSILGWDLKTPSSFLPQQNIENDNNNQGFEELGFHGMLGKQLSNVVDDDDDDVVVGSKIVTSSSFVMAAPNAFSEREQQHFNSKPKHSNSIGDTNSSNSLIDLKLGRFGDHRDGIGTPFSKGTTILSSSGSSTPSKRVRSSAVHSQIAYCQVYGCNKDLSGCKDYHKRHKVCEVHSKTSKVIVNGIEQRFCQQCSRFHLLAEFDDGKRSCRKRLAGHNERRRKPQVGIHSGRAGRLLQSYGDSRFQGTMLTSASFICQEILPGEVLSSEKCGNSNWCRPIKAEGGTGFRPLSSIPIINAHPHSRTLFPSYNNKQFPFLQENGATSATGSIFCENSSQYPPIQGGSNSGSQSLFQDTSLGSEDFNVFETESAVQGLSGVSDGGCALSLLSSQSQNSSSQSTGIPMPGHLVIPSSHSHYSMSHVSESGVSDRFPSELNHTDGSHLSPILISNSNEIAHFEMADGVFQGSDFVNVKDRLSCEDGTTIDLLQLSSQLQRVEHQRRALQVKRENDSSCSLRITKELLPVQLVVTGNIRIRIRT*