AlfalfaGEDB | Msa1421030

M. sativa cultivar zhongmu-4 / Msa1421030

KEGG: -
GO: -
PFAM: PF00240.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1421030	sp\|Q9ZPY9\|P4KG4_ARATH	58.319	595	207	9	1	588	1	561	0.0	682

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1421030	G8A2Y5	97.315	596	16	0	1	596	1	596	0.0	1206

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0706000	Msa1421030	0.806864	6.688565e-50	-8.615850e-47
Msa1421030	Msa1455140	0.950688	9.803201e-109	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1421030	MtrunA17_Chr8g0346901	97.315	596	16	0	1	596	1	596	0.0	1206
Msa1421030	MtrunA17_Chr7g0275661	70.352	597	163	5	1	590	1	590	0.0	834
Msa1421030	MtrunA17_Chr8g0388301	58.070	570	223	8	36	591	25	592	0.0	658
Msa1421030	MtrunA17_Chr3g0126781	58.070	570	215	6	30	588	19	575	0.0	652
Msa1421030	MtrunA17_Chr5g0396531	55.075	601	240	10	1	591	1	581	0.0	647
Msa1421030	MtrunA17_Chr8g0366091	40.411	292	144	7	270	547	150	425	2.24e-62	217
Msa1421030	MtrunA17_Chr7g0255481	38.816	304	158	7	258	547	76	365	8.11e-59	202
Msa1421030	MtrunA17_Chr5g0420771	39.731	297	141	8	269	547	155	431	2.96e-58	206
Msa1421030	MtrunA17_Chr7g0220391	39.236	288	142	7	277	547	155	426	1.73e-55	198
Msa1421030	MtrunA17_Chr7g0274921	32.544	169	102	6	26	189	67	228	7.41e-17	80.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1421030	AT2G46500.1	58.319	595	207	9	1	588	1	561	0.0	682
Msa1421030	AT2G46500.2	58.319	595	207	9	1	588	1	561	0.0	682
Msa1421030	AT5G24240.1	55.058	603	232	12	1	593	1	574	0.0	628
Msa1421030	AT5G24240.2	55.058	603	232	12	1	593	1	574	0.0	628
Msa1421030	AT2G46500.3	53.991	426	156	8	1	419	1	393	1.44e-144	425
Msa1421030	AT2G03890.1	39.871	311	154	8	254	547	139	433	3.38e-61	214
Msa1421030	AT1G13640.1	40.741	297	139	7	272	547	149	429	8.98e-61	213
Msa1421030	AT1G13640.2	40.741	297	139	7	272	547	149	429	8.98e-61	213
Msa1421030	AT1G26270.1	38.333	300	152	7	265	547	146	429	8.27e-57	202
Msa1421030	AT2G40850.1	38.636	308	161	6	280	572	120	414	1.68e-55	197

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Find 198 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTGGTTTGTAACGGG+CGG	0.617558	tig0024906:-30929	Msa1421030:CDS
AAACAAAAGTTGGTTTGTAA+CGG	0.520140	tig0024906:-30933	Msa1421030:CDS
AAAGAATGTGTTAAGACCAA+GGG	0.602876	tig0024906:+28598	None:intergenic
AAAGCATTTAACCATCCAGA+GGG	0.626201	tig0024906:-30006	Msa1421030:CDS
AAAGTAGCCTCACTAGTACC+AGG	0.600234	tig0024906:+28811	None:intergenic
AAAGTTGGTTTGTAACGGGC+GGG	0.575171	tig0024906:-30928	Msa1421030:CDS
AAATATGCGGATGTTAGTAC+AGG	0.514113	tig0024906:-30591	Msa1421030:CDS
AAATGAAGTGCTTACACTAG+TGG	0.555410	tig0024906:+29762	None:intergenic
AAATTGTTGTCTCCAACCAA+TGG	0.562278	tig0024906:-31110	Msa1421030:CDS
AACAAAAGTTGGTTTGTAAC+GGG	0.431768	tig0024906:-30932	Msa1421030:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGAATTGTGGAACAAGAT+AGG	+	tig0024906:28549-28568	None:intergenic	30.0%
ATTCAAGATTCGGAAAAGAA+GGG	+	tig0024906:28644-28663	None:intergenic	30.0%
AATTCAAGATTCGGAAAAGA+AGG	+	tig0024906:28645-28664	None:intergenic	30.0%
TGTTGTTGGAATTCAAGATT+CGG	+	tig0024906:28654-28673	None:intergenic	30.0%
CACAAAATTAGGGTTTATAG+AGG	-	tig0024906:28740-28759	Msa1421030:exon	30.0%
TTTCGTTTATCTTTCGTACT+CGG	-	tig0024906:29166-29185	Msa1421030:intron	30.0%
AGTGACAAACAAACAAAAGT+TGG	-	tig0024906:29265-29284	Msa1421030:intron	30.0%
CAAATGCAATACATTTCCTT+CGG	+	tig0024906:29348-29367	None:intergenic	30.0%
GTATGTTAAGAAACAGATTG+CGG	-	tig0024906:29460-29479	Msa1421030:intron	30.0%
AACAGGGCAAAAATATGTAT+CGG	-	tig0024906:29952-29971	Msa1421030:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0024906	gene	28480	31750	28480	ID=Msa1421030;Name=Msa1421030
tig0024906	mRNA	28480	31750	28480	ID=Msa1421030-mRNA-1;Parent=Msa1421030;Name=Msa1421030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=573\|1\|1\|1\|1\|1\|2\|264\|596
tig0024906	exon	28480	29133	28480	ID=Msa1421030-mRNA-1:exon:1884;Parent=Msa1421030-mRNA-1
tig0024906	exon	29777	31750	29777	ID=Msa1421030-mRNA-1:exon:1883;Parent=Msa1421030-mRNA-1
tig0024906	five_prime_UTR	31178	31750	31178	ID=Msa1421030-mRNA-1:five_prime_utr;Parent=Msa1421030-mRNA-1
tig0024906	CDS	29777	31177	29777	ID=Msa1421030-mRNA-1:cds;Parent=Msa1421030-mRNA-1
tig0024906	CDS	28744	29133	28744	ID=Msa1421030-mRNA-1:cds;Parent=Msa1421030-mRNA-1
tig0024906	three_prime_UTR	28480	28743	28480	ID=Msa1421030-mRNA-1:three_prime_utr;Parent=Msa1421030-mRNA-1

Gene Sequence

>Msa1421030

ATGTCTTCTGCTGGTGTAACCACTCTGAGTCCTGTTTCAAGGGAGAAATTGTTGTCTCCAACCAATGGATTATGTAATCCACTGCACTTGTCTTCGAATCTTCATGATTTTATTTTCGTTTATCTTTCGTACTCGGGGTCTTTGTCTCCGATGCGTGTGCTACCTTCGGATACAATTGAGTCAGTGAAACTCAAAATTCAGAGGAATGAAGGAGTGACAAACAAACAAAAGTTGGTTTGTAACGGGCGGGAGTTGTCGCGGAGCAATTCCTTGCTCAAGGATTATGGAGTTACCGAAGGAAATGTATTGCATTTGGTGATTCGACTGGCAGATCTACAGACCATCAGTGTGAGAACTTGTTCCGGGAAGGAGTATACCTTTCAGGTTGAAAGGTGTAAGGATGTTTGGTATGTTAAGAAACAGATTGCGGAAAAGGAAAATGAGTTTGCTGATCCTGAACAGCAGGAGGTTGTGTGTAACGGGGAGCTGCTTGATGATCAGAAGCTTATTGATGATATCTGTAGCAAATATAATGATGCAGTGATACATCTTTTTGTTAGAACAAAATATGCGGATGTTAGTACAGGGCTTGATGAGTTGTCTATTGAAGCAAAAGAATTGAATGATATGAAGAATTCTGATGCAGAGGAAGGTGATTATGGGAGGAAATATGATAGCAAAGAAGATGCGAGAAAGGAGTATGAAGCCATCGAACGGATTATGTCGAGAAATGCTCCTGGTAGAGGTCTTATTTTGGAGCCGGTTGTTGTTAACCCTAAAGTTGAACTAGCTTCAGAGGTTTGGGATATGATAAACACTACATATGATGGGCTAGATAGTGGTAATTCTCCAATCAGATCTGCAGAGGGTACAGGTGGGGCTTACTTTATGCTCGACTCAACAGGGCAAAAATATGTATCGGTTTTCAAGCCTATGGATGAAGAGCCGATGGCTGTGAATAACCCTAGAGGTTTACCTTTGTCGTTAGACGGTGAGGGCTTAAAAAAGGGTACAAGAGTTGGCCAAGGAGCTTTCAGGGAAGTTGCTGCTTATATTTTGGATCATCCAATGAGTGGCCGCCGATCATTATTTGGCCATGAGAAAGGATTTGCTGGTGTTCCTCCGACTGTTATGGCAAAGTGCTTGCACAAAGCATTTAACCATCCAGAGGGTTTGGCTGCTAAGTTTGGTTCCTTGCAAATGTTCGTGGAGAATAATGGAAGTTGTGAGGATATGGGACCTGGTGCTTTTCCGGTGAAGGAGGTGCATAAAATTACTGTGCTGGACATGAGACTTGCAAATGCAGATAGGCATGCTGGGAATATTTTGCTTGGCAAAGAGAAGAAAAATGGCCAGGCTGTTTTGATTCCAATTGATCATGGATACTGCCTTCCCACTAGTTTTGAAGATTGTACCTTTGAATGGCTTTATTGGCCTCAAGCTCGCCAGCCATACTCTCCAGAGATCATTGACTACATAAAGTCCCTTGATGCTGAAGAAGATATTGCCCTTCTGAAGTTTCATGGATGGGATCTGCCACTCGAATGCGCCCGCACTCTCCGAATCTCGACCATGCTTCTGAAAAAAGGAGTGGGGAAAGGGATGACCCCTTTTGCCATTGGAAGCCTGATGTGCCGAGAATCATTGAACAAAGAGTCTGTGATTGAAGGAATTTTTAAAGCAGCACTCGATTCTGTTCTTCCTGGTACTAGTGAGGCTACTTTTATGGATGCTGTTTCTGAGATTATGGACCAGCACCTTGATGAGATCACCAGCAATTATAATTCTTAA

Protein sequence

>Msa1421030

MSSAGVTTLSPVSREKLLSPTNGLCNPLHLSSNLHDFIFVYLSYSGSLSPMRVLPSDTIESVKLKIQRNEGVTNKQKLVCNGRELSRSNSLLKDYGVTEGNVLHLVIRLADLQTISVRTCSGKEYTFQVERCKDVWYVKKQIAEKENEFADPEQQEVVCNGELLDDQKLIDDICSKYNDAVIHLFVRTKYADVSTGLDELSIEAKELNDMKNSDAEEGDYGRKYDSKEDARKEYEAIERIMSRNAPGRGLILEPVVVNPKVELASEVWDMINTTYDGLDSGNSPIRSAEGTGGAYFMLDSTGQKYVSVFKPMDEEPMAVNNPRGLPLSLDGEGLKKGTRVGQGAFREVAAYILDHPMSGRRSLFGHEKGFAGVPPTVMAKCLHKAFNHPEGLAAKFGSLQMFVENNGSCEDMGPGAFPVKEVHKITVLDMRLANADRHAGNILLGKEKKNGQAVLIPIDHGYCLPTSFEDCTFEWLYWPQARQPYSPEIIDYIKSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGVGKGMTPFAIGSLMCRESLNKESVIEGIFKAALDSVLPGTSEATFMDAVSEIMDQHLDEITSNYNS*