AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1422550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1422550 MtrunA17_Chr1g0203721 94.541 458 23 1 187 644 428 883 0.0 874
Msa1422550 MtrunA17_Chr1g0203721 93.229 192 13 0 1 192 1 192 1.53e-121 382
Msa1422550 MtrunA17_Chr6g0458391 47.938 194 101 0 190 383 573 766 4.56e-56 203
Msa1422550 MtrunA17_Chr6g0458391 51.562 128 60 1 59 186 221 346 2.70e-34 139
Msa1422550 MtrunA17_Chr6g0458391 52.756 127 55 3 65 190 93 215 2.42e-32 133
Msa1422550 MtrunA17_Chr3g0082121 40.299 268 151 3 179 445 390 649 1.00e-42 164
Msa1422550 MtrunA17_Chr3g0082121 52.632 95 44 1 69 162 49 143 2.32e-23 105
Msa1422550 MtrunA17_Chr2g0324011 32.857 210 140 1 192 400 377 586 4.58e-34 137
Msa1422550 MtrunA17_Chr5g0448511 25.153 326 232 4 180 499 189 508 4.41e-27 115
Msa1422550 MtrunA17_Chr5g0448761 26.102 295 201 4 190 476 432 717 3.47e-26 114
Msa1422550 MtrunA17_Chr5g0448761 37.736 106 62 2 66 167 63 168 4.30e-12 69.3
Msa1422550 MtrunA17_Chr3g0118611 27.536 276 186 6 187 458 307 572 6.97e-25 110
Msa1422550 MtrunA17_Chr5g0401121 49.474 95 47 1 69 162 62 156 3.05e-24 99.8
Msa1422550 MtrunA17_Chr8g0363841 31.220 205 128 4 186 382 378 577 1.25e-23 105
Msa1422550 MtrunA17_Chr5g0440451 27.206 272 184 5 191 458 414 675 8.34e-23 103
Msa1422550 MtrunA17_Chr2g0329031 26.772 254 170 5 191 439 371 613 9.69e-22 100
Msa1422550 MtrunA17_Chr2g0329031 40.000 95 54 2 69 163 20 111 1.17e-11 68.2
Msa1422550 MtrunA17_Chr1g0202341 25.368 272 199 3 186 454 412 682 1.42e-21 99.4
Msa1422550 MtrunA17_Chr1g0207551 28.916 249 170 3 192 439 399 641 2.01e-21 99.4
Msa1422550 MtrunA17_Chr4g0033611 35.032 157 90 5 27 180 47 194 4.86e-19 87.4
Msa1422550 MtrunA17_Chr3g0080351 26.190 252 179 2 187 437 428 673 7.94e-19 91.3
Msa1422550 MtrunA17_Chr3g0080351 36.036 111 58 1 66 163 79 189 1.20e-12 71.2
Msa1422550 MtrunA17_Chr2g0329421 37.755 98 60 1 65 162 29 125 2.22e-16 78.6
Msa1422550 MtrunA17_Chr2g0287061 26.538 260 183 3 179 436 499 752 4.70e-16 82.0
Msa1422550 MtrunA17_Chr2g0287061 36.364 99 50 1 65 163 185 270 1.55e-12 70.9
Msa1422550 MtrunA17_Chr2g0287061 37.374 99 49 1 65 163 31 116 7.36e-12 68.6
Msa1422550 MtrunA17_Chr2g0291581 27.451 204 140 3 187 382 418 621 1.46e-15 80.5
Msa1422550 MtrunA17_Chr5g0441581 22.593 270 194 7 187 445 382 647 1.93e-15 80.1
Msa1422550 MtrunA17_Chr7g0233911 31.343 134 78 2 65 184 36 169 7.25e-15 74.3
Msa1422550 MtrunA17_Chr1g0198761 22.381 210 142 4 181 383 418 613 3.01e-13 73.2
Msa1422550 MtrunA17_Chr2g0308841 21.698 212 164 2 176 385 19 230 4.61e-13 69.7
Msa1422550 MtrunA17_Chr7g0238821 36.111 108 66 3 69 175 3 108 1.63e-12 64.3
Msa1422550 MtrunA17_Chr2g0329361 36.111 108 61 2 65 167 63 167 2.97e-12 67.4
Msa1422550 MtrunA17_Chr7g0258861 33.636 110 71 2 65 173 12 120 3.08e-12 68.2
Msa1422550 MtrunA17_Chr3g0078041 37.755 98 60 1 66 162 53 150 8.24e-12 65.5
Msa1422550 MtrunA17_Chr2g0287651 22.791 215 159 4 176 386 424 635 1.09e-11 68.2
Msa1422550 MtrunA17_Chr2g0287651 34.314 102 63 3 69 167 81 181 9.43e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1422550 AT4G38170.1 25.597 293 195 3 187 478 203 473 9.76e-29 121
Msa1422550 AT3G22170.2 26.471 272 194 2 186 457 422 687 4.86e-26 114
Msa1422550 AT3G22170.2 28.415 183 95 4 2 163 16 183 5.94e-12 69.3
Msa1422550 AT3G22170.1 26.471 272 194 2 186 457 422 687 4.86e-26 114
Msa1422550 AT3G22170.1 28.415 183 95 4 2 163 16 183 5.94e-12 69.3
Msa1422550 AT2G32250.2 28.214 280 187 6 190 463 361 632 1.62e-25 112
Msa1422550 AT2G32250.2 37.755 98 58 1 66 163 41 135 2.06e-11 67.8
Msa1422550 AT2G32250.4 28.214 280 187 6 190 463 361 632 1.62e-25 112
Msa1422550 AT2G32250.4 37.755 98 58 1 66 163 41 135 2.06e-11 67.8
Msa1422550 AT2G32250.6 28.214 280 187 6 190 463 361 632 1.74e-25 112
Msa1422550 AT2G32250.6 37.755 98 58 1 66 163 41 135 2.08e-11 67.8
Msa1422550 AT2G32250.1 28.214 280 187 6 190 463 361 632 1.74e-25 112
Msa1422550 AT2G32250.1 37.755 98 58 1 66 163 41 135 2.08e-11 67.8
Msa1422550 AT2G32250.3 28.214 280 187 6 190 463 361 632 1.74e-25 112
Msa1422550 AT2G32250.3 37.755 98 58 1 66 163 41 135 2.08e-11 67.8
Msa1422550 AT2G32250.8 28.214 280 187 6 190 463 320 591 2.33e-25 112
Msa1422550 AT2G32250.8 37.895 95 56 1 69 163 3 94 5.04e-11 66.6
Msa1422550 AT2G32250.7 28.214 280 187 6 190 463 320 591 2.36e-25 112
Msa1422550 AT2G32250.7 37.895 95 56 1 69 163 3 94 5.14e-11 66.2
Msa1422550 AT2G32250.5 28.214 280 187 6 190 463 361 632 2.39e-25 112
Msa1422550 AT2G32250.5 37.755 98 58 1 66 163 41 135 1.84e-11 67.8
Msa1422550 AT4G38180.1 30.412 194 134 1 190 382 441 634 1.05e-24 110
Msa1422550 AT4G38180.1 33.143 175 97 3 13 167 6 180 7.30e-16 82.0
Msa1422550 AT4G15090.1 23.195 457 289 11 186 636 413 813 6.91e-24 107
Msa1422550 AT4G15090.5 23.195 457 289 11 186 636 413 813 6.91e-24 107
Msa1422550 AT4G15090.3 23.195 457 289 11 186 636 413 813 6.91e-24 107
Msa1422550 AT4G15090.2 23.195 457 289 11 186 636 413 813 6.91e-24 107
Msa1422550 AT4G15090.4 23.195 457 289 11 186 636 413 813 6.91e-24 107
Msa1422550 AT2G27110.2 27.586 261 180 5 189 448 387 639 9.35e-22 100
Msa1422550 AT2G27110.1 27.586 261 180 5 189 448 387 639 9.35e-22 100
Msa1422550 AT2G27110.3 27.586 261 180 5 189 448 242 494 1.40e-21 100
Msa1422550 AT3G06250.2 28.405 257 172 5 190 444 514 760 5.60e-21 98.2
Msa1422550 AT3G06250.2 34.783 115 61 2 66 180 193 293 2.33e-12 70.9
Msa1422550 AT3G06250.2 35.354 99 51 1 65 163 30 115 4.40e-12 69.7
Msa1422550 AT3G06250.1 28.405 257 172 5 190 444 514 760 5.60e-21 98.2
Msa1422550 AT3G06250.1 34.783 115 61 2 66 180 193 293 2.33e-12 70.9
Msa1422550 AT3G06250.1 35.354 99 51 1 65 163 30 115 4.40e-12 69.7
Msa1422550 AT3G06250.3 28.405 257 172 5 190 444 514 760 5.60e-21 98.2
Msa1422550 AT3G06250.3 34.783 115 61 2 66 180 193 293 2.33e-12 70.9
Msa1422550 AT3G06250.3 35.354 99 51 1 65 163 30 115 4.40e-12 69.7
Msa1422550 AT5G18960.2 27.027 259 169 6 192 444 540 784 4.48e-20 95.5
Msa1422550 AT5G18960.2 30.464 151 84 3 13 163 1 130 1.34e-12 71.6
Msa1422550 AT5G18960.2 38.679 106 51 2 66 171 214 305 1.36e-12 71.6
Msa1422550 AT5G18960.1 27.027 259 169 6 192 444 540 784 4.48e-20 95.5
Msa1422550 AT5G18960.1 30.464 151 84 3 13 163 1 130 1.34e-12 71.6
Msa1422550 AT5G18960.1 38.679 106 51 2 66 171 214 305 1.36e-12 71.6
Msa1422550 AT1G76320.2 25.200 250 176 3 192 439 356 596 1.68e-19 93.6
Msa1422550 AT1G76320.1 25.200 250 176 3 192 439 356 596 1.82e-19 93.6
Msa1422550 AT1G76320.4 25.200 250 176 3 192 439 356 596 1.82e-19 93.6
Msa1422550 AT1G76320.3 25.200 250 176 3 192 439 356 596 4.65e-19 92.0
Msa1422550 AT3G07500.1 42.593 108 61 1 65 172 35 141 1.90e-18 85.1
Msa1422550 AT4G19990.6 28.520 277 190 4 190 462 365 637 2.95e-18 89.7
Msa1422550 AT4G19990.2 28.520 277 190 4 190 462 365 637 2.95e-18 89.7
Msa1422550 AT1G52520.1 27.509 269 182 6 186 444 433 698 2.96e-18 89.4
Msa1422550 AT4G19990.5 28.520 277 190 4 190 462 365 637 3.51e-18 89.4
Msa1422550 AT4G19990.3 28.520 277 190 4 190 462 365 637 3.83e-18 89.4
Msa1422550 AT4G19990.4 28.520 277 190 4 190 462 365 637 3.83e-18 89.4
Msa1422550 AT4G19990.7 28.520 277 190 4 190 462 379 651 4.17e-18 89.0
Msa1422550 AT4G19990.1 28.520 277 190 4 190 462 308 580 4.26e-18 89.0
Msa1422550 AT3G59470.3 39.796 98 58 1 65 162 72 168 5.49e-18 84.7
Msa1422550 AT3G59470.1 39.796 98 58 1 65 162 72 168 5.49e-18 84.7
Msa1422550 AT3G59470.2 39.796 98 58 1 65 162 84 180 7.14e-18 84.3
Msa1422550 AT2G43280.1 36.735 98 61 1 66 162 25 122 2.53e-13 69.7
Msa1422550 AT4G12850.4 32.653 98 63 1 65 162 13 107 2.87e-13 67.8
Msa1422550 AT4G12850.2 32.653 98 63 1 65 162 13 107 6.80e-12 63.9
Msa1422550 AT4G12850.1 32.653 98 63 1 65 162 13 107 1.19e-11 64.3
Msa1422550 AT1G80010.1 24.762 210 151 4 180 382 452 661 3.98e-11 66.6

Find 145 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTCGGACTGCAACAATTT+TGG 0.148412 tig0025415:-66039 None:intergenic
AGGATTCTGAATGAGGATTT+TGG 0.176822 tig0025415:-68948 None:intergenic
TAATATGAATGATGAATATT+TGG 0.181624 tig0025415:-69161 None:intergenic
AAGTGTATTTGATCTAAATT+TGG 0.221536 tig0025415:+69205 Msa1422550:three_prime_UTR
CAGCTTGCTACAACCATCTT+AGG 0.224643 tig0025415:-67861 None:intergenic
CGGACTCATGGTATGTGTTT+TGG 0.233173 tig0025415:-68765 None:intergenic
GATTATGTTGTGCTTCAAAA+TGG 0.267872 tig0025415:-66408 None:intergenic
ATGCAAGGTTGGGTAATTTC+AGG 0.281403 tig0025415:+67808 Msa1422550:CDS
TTAGAAAAGATTGGATTAAT+AGG 0.287407 tig0025415:+66215 Msa1422550:CDS
GGCATTGCCAATACAGGATA+TGG 0.302964 tig0025415:-69041 None:intergenic
CTTACCTGCAAAGGGAGATT+TGG 0.310345 tig0025415:-68287 None:intergenic
CCTCCACTATCCTTTCAAAA+TGG 0.316329 tig0025415:-67401 None:intergenic
ACTTGGAACATCCAATAGTT+TGG 0.334138 tig0025415:+66003 Msa1422550:CDS
CACATTCAAGTCCAAACTAT+TGG 0.341769 tig0025415:-66014 None:intergenic
CATGCATTGAACCACTTCAT+TGG 0.347987 tig0025415:-68538 None:intergenic
CCGAACGTTCTTCACGCTAA+TGG 0.356305 tig0025415:+66055 Msa1422550:CDS
AACAACATGTTAGAATTGAT+TGG 0.361206 tig0025415:-66445 None:intergenic
TACATGGTATGGTGGTTTCT+AGG 0.366745 tig0025415:+66245 Msa1422550:CDS
TCAACCAAATCTCCCTTTGC+AGG 0.367806 tig0025415:+68283 Msa1422550:CDS
GGCTTTGTCAACACAGTGTT+CGG 0.372860 tig0025415:+66115 Msa1422550:CDS
GGTTCAATGTTAGAAAAGAT+TGG 0.384400 tig0025415:+66206 Msa1422550:CDS
CCACAGACTTGTCAAGTTAT+CGG 0.386090 tig0025415:+67899 Msa1422550:CDS
GATGTCAAAGGCTTTCATTT+AGG 0.388977 tig0025415:+67312 Msa1422550:CDS
GTATAGAGAATGGGAGAAAT+GGG 0.389543 tig0025415:+67257 Msa1422550:CDS
GGGATGTCTTCCAAAGCTAA+TGG 0.397500 tig0025415:+67464 Msa1422550:CDS
TGAAATATTTCAAATGCTCT+TGG 0.400052 tig0025415:-67528 None:intergenic
TGTATAGAGAATGGGAGAAA+TGG 0.401167 tig0025415:+67256 Msa1422550:CDS
TTTGGTTGAGATGAAACATA+TGG 0.404147 tig0025415:-68269 None:intergenic
AGAAGTTTGGCGGTTGGAAC+AGG 0.408816 tig0025415:-68605 None:intergenic
TGTTGGTTAGAATAGAAGTT+TGG 0.411083 tig0025415:-68618 None:intergenic
GAGTATAAAGTGAATACATT+TGG 0.412707 tig0025415:+67615 Msa1422550:CDS
TGTATTATCTGAAGAATTGA+AGG 0.418492 tig0025415:-67661 None:intergenic
ACCACATTACCCATTAGCTT+TGG 0.425279 tig0025415:-67474 None:intergenic
AACATTGAACCCCATCAATC+TGG 0.425557 tig0025415:-66194 None:intergenic
ACAACATGTTAGAATTGATT+GGG 0.429453 tig0025415:-66444 None:intergenic
AGTCAGAACTTCGGTATCTA+TGG 0.430265 tig0025415:-68987 None:intergenic
TGGACATGCGACTGAGGATC+AGG 0.436653 tig0025415:+68094 Msa1422550:CDS
AATAATGGCATTGCCAATAC+AGG 0.438574 tig0025415:-69047 None:intergenic
GGAGACGAGAACTGGATGTT+TGG 0.438770 tig0025415:+66339 Msa1422550:CDS
CTCTTCCCTTTGTTTAAGAC+CGG 0.439983 tig0025415:+69244 Msa1422550:three_prime_UTR
CATCGGAAGGAGACGAGAAC+TGG 0.445746 tig0025415:+66331 Msa1422550:CDS
GAGAAATGGGCTGTCTGCTT+TGG 0.447459 tig0025415:+67270 Msa1422550:CDS
TAACAAGTATGCCAGATTGA+TGG 0.451658 tig0025415:+66183 Msa1422550:CDS
AATGGCTCTCAAACTCAATC+TGG 0.454597 tig0025415:+66073 Msa1422550:CDS
ATAATCAAACTGATTCAGAT+AGG 0.455395 tig0025415:+68189 Msa1422550:CDS
AACAAGTATGCCAGATTGAT+GGG 0.458593 tig0025415:+66184 Msa1422550:CDS
TGATGATCTAACACAAGATC+AGG 0.463119 tig0025415:-68570 None:intergenic
TCCTCAGTCGCATGTCCATT+CGG 0.464228 tig0025415:-68089 None:intergenic
GTCAACACAGTGTTCGGAAT+CGG 0.464277 tig0025415:+66121 Msa1422550:CDS
GGATGTCTTCCAAAGCTAAT+GGG 0.465360 tig0025415:+67465 Msa1422550:CDS
AGAAGCTAGTGATGAAATGA+AGG 0.466016 tig0025415:+67443 Msa1422550:CDS
ATCTACATGAATACTATTTG+AGG 0.467176 tig0025415:-68036 None:intergenic
GAGATGGATGTATAGAGAAT+GGG 0.471472 tig0025415:+67248 Msa1422550:CDS
GGTCGAACCATATCCTGTAT+TGG 0.475597 tig0025415:+69034 Msa1422550:three_prime_UTR
CTCCCTCCGGTCTTAAACAA+AGG 0.475629 tig0025415:-69250 None:intergenic
TGTTGCATCTGATTATTGCT+TGG 0.479286 tig0025415:-68790 None:intergenic
TGATAAAAGCTTACCTGCAA+AGG 0.481590 tig0025415:-68296 None:intergenic
TGCAAGGTTGGGTAATTTCA+GGG 0.481977 tig0025415:+67809 Msa1422550:CDS
CAGACAACAGTATTTGAAGA+AGG 0.483624 tig0025415:-68227 None:intergenic
AGCTTGCTACAACCATCTTA+GGG 0.484144 tig0025415:-67860 None:intergenic
GAGGCAACTTGACGAAGTTA+TGG 0.484472 tig0025415:+67962 Msa1422550:CDS
AATTGAACTACATCAAGGTC+AGG 0.492101 tig0025415:-67372 None:intergenic
AATTCAAGATAACCCTAAGA+TGG 0.492105 tig0025415:+67848 Msa1422550:CDS
CTCGATATATCTTGAAGAGA+TGG 0.495398 tig0025415:+67778 Msa1422550:CDS
AATTGACAGGACCGCTACAC+TGG 0.496426 tig0025415:+68142 Msa1422550:CDS
AAGCAATAATCAGATGCAAC+AGG 0.498238 tig0025415:+68792 Msa1422550:CDS
ATGGTCACACAGCAAAATAC+AGG 0.502031 tig0025415:-68968 None:intergenic
TCTGGTAATGCATCAGCAAA+TGG 0.509074 tig0025415:+66091 Msa1422550:CDS
TAGGAGTAAGGTACATGGTA+TGG 0.509545 tig0025415:+66234 Msa1422550:CDS
ACGATATCCCTCTCTGGAGC+AGG 0.509622 tig0025415:-66275 None:intergenic
GATGGTGATCATAACAACTT+AGG 0.510043 tig0025415:+65950 Msa1422550:CDS
GATAAAAGCTTACCTGCAAA+GGG 0.510969 tig0025415:-68295 None:intergenic
CCATTAGCGTGAAGAACGTT+CGG 0.511332 tig0025415:-66055 None:intergenic
ATTATGTTGTGCTTCAAAAT+GGG 0.511977 tig0025415:-66407 None:intergenic
TGAGATGAAACATATGGAGA+TGG 0.517004 tig0025415:-68263 None:intergenic
GAGATGAAACATATGGAGAT+GGG 0.517572 tig0025415:-68262 None:intergenic
ATGGACAAAAGATGCAAGGT+TGG 0.517774 tig0025415:+67797 Msa1422550:CDS
TAGAATAGAAGTTTGGCGGT+TGG 0.518493 tig0025415:-68611 None:intergenic
TCCCTCCGGTCTTAAACAAA+GGG 0.519160 tig0025415:-69249 None:intergenic
AAAATACAGGATTCTGAATG+AGG 0.519697 tig0025415:-68955 None:intergenic
ATCATTACTCGTCAACAAGA+TGG 0.520020 tig0025415:+66370 Msa1422550:CDS
TGAGATGGATGTATAGAGAA+TGG 0.522848 tig0025415:+67247 Msa1422550:CDS
GAAAATGTTGATGTTGGACT+TGG 0.524439 tig0025415:+65986 Msa1422550:CDS
TTTCCTACGATATCCCTCTC+TGG 0.525239 tig0025415:-66281 None:intergenic
TATTGCTTGGCACGGACTCA+TGG 0.526252 tig0025415:-68777 None:intergenic
TGGTTAGAATAGAAGTTTGG+CGG 0.528921 tig0025415:-68615 None:intergenic
CATGTAGAAAATGTTGATGT+TGG 0.531700 tig0025415:+65980 Msa1422550:CDS
AGATTGGATTAATAGGAGTA+AGG 0.541743 tig0025415:+66222 Msa1422550:CDS
GATTATAATTCACCGTTGGT+TGG 0.543940 tig0025415:-68172 None:intergenic
CAATAGTTTGGACTTGAATG+TGG 0.544732 tig0025415:+66015 Msa1422550:CDS
TGGACAAAAGATGCAAGGTT+GGG 0.547513 tig0025415:+67798 Msa1422550:CDS
TTGCCTTTCTCAACTATCGC+AGG 0.548639 tig0025415:+68650 Msa1422550:CDS
TTGTGTTGCTCAAGCTGTGG+AGG 0.559431 tig0025415:+66489 Msa1422550:CDS
TTCCCTTTGTTTAAGACCGG+AGG 0.560342 tig0025415:+69247 Msa1422550:three_prime_UTR
GTTTGATTATAATTCACCGT+TGG 0.560586 tig0025415:-68176 None:intergenic
ATAAACTCCAGCGAAATGAA+TGG 0.563219 tig0025415:+67223 Msa1422550:intron
GTCAACCAGCACAAAAGAGA+TGG 0.564837 tig0025415:+67582 Msa1422550:CDS
ATATTAAAAGATACCCCGTC+CGG 0.573728 tig0025415:+69178 Msa1422550:three_prime_UTR
AGATCAAATACACTTAAGAC+CGG 0.574251 tig0025415:-69197 None:intergenic
TAAGAAATTGAACTACATCA+AGG 0.574535 tig0025415:-67377 None:intergenic
AAAGGCAAGGAGAACTCTGT+TGG 0.577643 tig0025415:-68635 None:intergenic
CACAGTGTTCGGAATCGGTA+CGG 0.578865 tig0025415:+66126 Msa1422550:CDS
TCCAAAGCTAATGGGTAATG+TGG 0.580465 tig0025415:+67473 Msa1422550:CDS
AATCTTGATATTGAAAAGTG+AGG 0.582977 tig0025415:+67998 Msa1422550:CDS
ACAGGACCGCTACACTGGAT+AGG 0.587540 tig0025415:+68147 Msa1422550:CDS
AAATTTACCTGCTCCAGAGA+GGG 0.588352 tig0025415:+66268 Msa1422550:CDS
AAGAGCTTCTGAATCTGTGG+AGG 0.588474 tig0025415:+67923 Msa1422550:CDS
TACTGTGAAGAAACATCGGA+AGG 0.591189 tig0025415:+66318 Msa1422550:CDS
AGCGAAATGAATGGTTGAGA+TGG 0.591609 tig0025415:+67232 Msa1422550:intron
AAATACACTTAAGACCGGAC+GGG 0.592534 tig0025415:-69192 None:intergenic
CCATTCGGAAAGATCACCGC+AGG 0.593497 tig0025415:-68074 None:intergenic
GAGTAAGCGAAGAAATTGAC+AGG 0.593964 tig0025415:+68129 Msa1422550:CDS
ACACCTGCGATAGTTGAGAA+AGG 0.595685 tig0025415:-68653 None:intergenic
GGCAAGAGCTTCTGAATCTG+TGG 0.597094 tig0025415:+67920 Msa1422550:CDS
GAGTAAGGTACATGGTATGG+TGG 0.599166 tig0025415:+66237 Msa1422550:CDS
CATCTGATTATTGCTTGGCA+CGG 0.603767 tig0025415:-68785 None:intergenic
CAAATACACTTAAGACCGGA+CGG 0.604119 tig0025415:-69193 None:intergenic
TCCGAATGGACATGCGACTG+AGG 0.607571 tig0025415:+68088 Msa1422550:CDS
ACTGGATAGGTGCCAACCAA+CGG 0.611215 tig0025415:+68160 Msa1422550:CDS
AGATGAAACATATGGAGATG+GGG 0.614637 tig0025415:-68261 None:intergenic
AATACACTTAAGACCGGACG+GGG 0.617779 tig0025415:-69191 None:intergenic
TGTATGAAGTTTGAGCATGT+AGG 0.618522 tig0025415:+67696 Msa1422550:CDS
CTCTTCAAGATATATCGAGA+TGG 0.620754 tig0025415:-67774 None:intergenic
TCAACAAGATGGAAAATACC+AGG 0.623065 tig0025415:+66381 Msa1422550:CDS
CCTGCGGTGATCTTTCCGAA+TGG 0.627373 tig0025415:+68074 Msa1422550:CDS
GTGCTTCAAAATGGGTAACC+TGG 0.628259 tig0025415:-66399 None:intergenic
GACTCTGCATAACGTATCAG+AGG 0.628578 tig0025415:+69006 Msa1422550:exon
CATAACGTATCAGAGGATCA+TGG 0.631520 tig0025415:+69013 Msa1422550:exon
GATGAAACATATGGAGATGG+GGG 0.631905 tig0025415:-68260 None:intergenic
AGAGATGGACAAAAGATGCA+AGG 0.632488 tig0025415:+67793 Msa1422550:CDS
TGCTTCAGAAAGTGAGCCTG+CGG 0.635679 tig0025415:+68058 Msa1422550:CDS
CAACTTGACGAAGTTATGGA+AGG 0.645660 tig0025415:+67966 Msa1422550:CDS
GAAGCTAGTGATGAAATGAA+GGG 0.646836 tig0025415:+67444 Msa1422550:CDS
CCGATAACTTGACAAGTCTG+TGG 0.655057 tig0025415:-67899 None:intergenic
ATTAATAGGAGTAAGGTACA+TGG 0.662022 tig0025415:+66229 Msa1422550:CDS
TGCGATAGTTGAGAAAGGCA+AGG 0.663759 tig0025415:-68648 None:intergenic
AGAGCTTCTGAATCTGTGGA+GGG 0.666121 tig0025415:+67924 Msa1422550:CDS
GAAATTTACCTGCTCCAGAG+AGG 0.668278 tig0025415:+66267 Msa1422550:CDS
GGTAATGCATCAGCAAATGG+AGG 0.677979 tig0025415:+66094 Msa1422550:CDS
GTTATGCAGAGTCAGAACTT+CGG 0.680514 tig0025415:-68996 None:intergenic
TTGGCACCTATCCAGTGTAG+CGG 0.682153 tig0025415:-68153 None:intergenic
ACAAGTATGCCAGATTGATG+GGG 0.683870 tig0025415:+66185 Msa1422550:CDS
AGGGTTATCAGTTTGCTGTG+AGG 0.684882 tig0025415:+67943 Msa1422550:CDS
ATGAAACATATGGAGATGGG+GGG 0.693657 tig0025415:-68259 None:intergenic
GCTCCAGAGAGGGATATCGT+AGG 0.703657 tig0025415:+66278 Msa1422550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATTTATTTGATTGATATA+TGG + tig0025415:68316-68335 Msa1422550:intron 15.0%
!! GAAATAAAAAATAAAGAGAA+CGG + tig0025415:68466-68485 Msa1422550:intron 15.0%
!! TTGGTTATTTACAAAAAAAA+AGG - tig0025415:69145-69164 None:intergenic 15.0%
!! TAATATGAATGATGAATATT+TGG - tig0025415:69164-69183 None:intergenic 15.0%
!! TTAGAAAAGATTGGATTAAT+AGG + tig0025415:66215-66234 Msa1422550:CDS 20.0%
!! AAGATAATTGTCATAATTCA+TGG - tig0025415:66608-66627 None:intergenic 20.0%
!!! AATTTCTTAACCATTTTGAA+AGG + tig0025415:67391-67410 Msa1422550:CDS 20.0%
!!! ATTTTCTCATTTGTTTTACA+GGG + tig0025415:68498-68517 Msa1422550:intron 20.0%
!! TATAAAAAAAACCTAATGAC+TGG - tig0025415:68687-68706 None:intergenic 20.0%
!!! ATATCATGAATATTTTACTC+AGG + tig0025415:68732-68751 Msa1422550:intron 20.0%
!!! ATTATTTTTGTTGTACAGTT+GGG + tig0025415:69064-69083 Msa1422550:three_prime_UTR 20.0%
!! AAGTGTATTTGATCTAAATT+TGG + tig0025415:69205-69224 Msa1422550:three_prime_UTR 20.0%
!! ATTTTACTGTGAAGAAACAT+CGG + tig0025415:66314-66333 Msa1422550:CDS 25.0%
! ATTATGTTGTGCTTCAAAAT+GGG - tig0025415:66410-66429 None:intergenic 25.0%
! ACAACATGTTAGAATTGATT+GGG - tig0025415:66447-66466 None:intergenic 25.0%
! AACAACATGTTAGAATTGAT+TGG - tig0025415:66448-66467 None:intergenic 25.0%
!! GTTGACTCATATCATAATTT+GGG + tig0025415:66511-66530 Msa1422550:intron 25.0%
! AGTATGACGAATACAAAATT+AGG + tig0025415:66556-66575 Msa1422550:intron 25.0%
!! ACAAGATAACAAATCTTTTC+TGG + tig0025415:66746-66765 Msa1422550:intron 25.0%
!! CAAGATAACAAATCTTTTCT+GGG + tig0025415:66747-66766 Msa1422550:intron 25.0%
!!! AGATTTTTTGTAGATGTCAA+AGG + tig0025415:67300-67319 Msa1422550:CDS 25.0%
! TAAGAAATTGAACTACATCA+AGG - tig0025415:67380-67399 None:intergenic 25.0%
! TGAAATATTTCAAATGCTCT+TGG - tig0025415:67531-67550 None:intergenic 25.0%
! GAGTATAAAGTGAATACATT+TGG + tig0025415:67615-67634 Msa1422550:CDS 25.0%
! TGTATTATCTGAAGAATTGA+AGG - tig0025415:67664-67683 None:intergenic 25.0%
! AATCTTGATATTGAAAAGTG+AGG + tig0025415:67998-68017 Msa1422550:CDS 25.0%
! ATCTACATGAATACTATTTG+AGG - tig0025415:68039-68058 None:intergenic 25.0%
!! ATAATCAAACTGATTCAGAT+AGG + tig0025415:68189-68208 Msa1422550:CDS 25.0%
!!! GATTTTCTCATTTGTTTTAC+AGG + tig0025415:68497-68516 Msa1422550:intron 25.0%
!! TGTTAATTTTTCCAGTCATT+AGG + tig0025415:68673-68692 Msa1422550:intron 25.0%
! ACAGGTAAACATTTACTTAA+TGG + tig0025415:68810-68829 Msa1422550:intron 25.0%
! CAACTGTACAACAAAAATAA+TGG - tig0025415:69065-69084 None:intergenic 25.0%
!!! CATTATTTTTGTTGTACAGT+TGG + tig0025415:69063-69082 Msa1422550:three_prime_UTR 25.0%
CATGTAGAAAATGTTGATGT+TGG + tig0025415:65980-65999 Msa1422550:CDS 30.0%
GGTTCAATGTTAGAAAAGAT+TGG + tig0025415:66206-66225 Msa1422550:CDS 30.0%
AGATTGGATTAATAGGAGTA+AGG + tig0025415:66222-66241 Msa1422550:CDS 30.0%
ATTAATAGGAGTAAGGTACA+TGG + tig0025415:66229-66248 Msa1422550:CDS 30.0%
GATTATGTTGTGCTTCAAAA+TGG - tig0025415:66411-66430 None:intergenic 30.0%
! GGTTGACTCATATCATAATT+TGG + tig0025415:66510-66529 Msa1422550:intron 30.0%
CTTAAAGCTGAACTAGATTT+GGG - tig0025415:66538-66557 None:intergenic 30.0%
ACTTAAAGCTGAACTAGATT+TGG - tig0025415:66539-66558 None:intergenic 30.0%
ACAATTATCTTCATTCTGTG+AGG + tig0025415:66617-66636 Msa1422550:intron 30.0%
CAATTATCTTCATTCTGTGA+GGG + tig0025415:66618-66637 Msa1422550:intron 30.0%
!!! GTTTGATGGGTTATTTTCAA+AGG + tig0025415:66671-66690 Msa1422550:intron 30.0%
TGTATGGCATAAAAAAATGC+TGG - tig0025415:66712-66731 None:intergenic 30.0%
ACACCTAAAAACTGTACAAA+TGG - tig0025415:66859-66878 None:intergenic 30.0%
AGACCATCGAATCATTTAAT+TGG + tig0025415:66929-66948 Msa1422550:intron 30.0%
ATTCGTCATCTTTAACCAAA+TGG - tig0025415:67116-67135 None:intergenic 30.0%
AATCCTTTGCAAATGATCTA+AGG + tig0025415:67133-67152 Msa1422550:intron 30.0%
!! TAACCATTTTGAAAGGATAG+TGG + tig0025415:67398-67417 Msa1422550:CDS 30.0%
! TATCAAGAACTTTTAGTGCA+TGG - tig0025415:67734-67753 None:intergenic 30.0%
AATTCAAGATAACCCTAAGA+TGG + tig0025415:67848-67867 Msa1422550:CDS 30.0%
GTTTGATTATAATTCACCGT+TGG - tig0025415:68179-68198 None:intergenic 30.0%
TTTGGTTGAGATGAAACATA+TGG - tig0025415:68272-68291 None:intergenic 30.0%
GCATATTTGATATAGAGATC+AGG + tig0025415:68366-68385 Msa1422550:intron 30.0%
! GTTTTATGCTCGTTAACTTT+TGG + tig0025415:68421-68440 Msa1422550:intron 30.0%
!!! TTTGTTTTACAGGGCTTATT+TGG + tig0025415:68507-68526 Msa1422550:intron 30.0%
! TGTTGGTTAGAATAGAAGTT+TGG - tig0025415:68621-68640 None:intergenic 30.0%
!! TGGTATGTGTTTTGGATTAT+AGG - tig0025415:68760-68779 None:intergenic 30.0%
!! CTTTGTGTATATTTGCTGTA+GGG + tig0025415:68910-68929 Msa1422550:intron 30.0%
AAAATACAGGATTCTGAATG+AGG - tig0025415:68958-68977 None:intergenic 30.0%
AGATCAAATACACTTAAGAC+CGG - tig0025415:69200-69219 None:intergenic 30.0%
GATGGTGATCATAACAACTT+AGG + tig0025415:65950-65969 Msa1422550:CDS 35.0%
GAAAATGTTGATGTTGGACT+TGG + tig0025415:65986-66005 Msa1422550:CDS 35.0%
ACTTGGAACATCCAATAGTT+TGG + tig0025415:66003-66022 Msa1422550:CDS 35.0%
CACATTCAAGTCCAAACTAT+TGG - tig0025415:66017-66036 None:intergenic 35.0%
CAATAGTTTGGACTTGAATG+TGG + tig0025415:66015-66034 Msa1422550:CDS 35.0%
TAACAAGTATGCCAGATTGA+TGG + tig0025415:66183-66202 Msa1422550:CDS 35.0%
AACAAGTATGCCAGATTGAT+GGG + tig0025415:66184-66203 Msa1422550:CDS 35.0%
ATCATTACTCGTCAACAAGA+TGG + tig0025415:66370-66389 Msa1422550:CDS 35.0%
TCAACAAGATGGAAAATACC+AGG + tig0025415:66381-66400 Msa1422550:CDS 35.0%
! TGGTAGATTACGACCATTTT+GGG + tig0025415:66785-66804 Msa1422550:intron 35.0%
AACAAACAACATCCCCAAAA+TGG - tig0025415:66801-66820 None:intergenic 35.0%
!! TTTTGGGGATGTTGTTTGTT+TGG + tig0025415:66801-66820 Msa1422550:intron 35.0%
CAGCCAATTAAATGATTCGA+TGG - tig0025415:66935-66954 None:intergenic 35.0%
AGCATCTTGTTCTGTGATAA+TGG - tig0025415:67001-67020 None:intergenic 35.0%
AATCATCGAAGCAATCAATG+TGG + tig0025415:67023-67042 Msa1422550:intron 35.0%
TCTTAAACATCTCAGCCATT+TGG + tig0025415:67098-67117 Msa1422550:intron 35.0%
ACTCCTTAGATCATTTGCAA+AGG - tig0025415:67139-67158 None:intergenic 35.0%
AGGAAGAATTTACTCATGCT+TGG + tig0025415:67181-67200 Msa1422550:intron 35.0%
GGAAGAATTTACTCATGCTT+GGG + tig0025415:67182-67201 Msa1422550:intron 35.0%
GAAGAATTTACTCATGCTTG+GGG + tig0025415:67183-67202 Msa1422550:intron 35.0%
AAGAATTTACTCATGCTTGG+GGG + tig0025415:67184-67203 Msa1422550:intron 35.0%
ATAAACTCCAGCGAAATGAA+TGG + tig0025415:67223-67242 Msa1422550:intron 35.0%
TGAGATGGATGTATAGAGAA+TGG + tig0025415:67247-67266 Msa1422550:CDS 35.0%
GAGATGGATGTATAGAGAAT+GGG + tig0025415:67248-67267 Msa1422550:CDS 35.0%
TGTATAGAGAATGGGAGAAA+TGG + tig0025415:67256-67275 Msa1422550:CDS 35.0%
GTATAGAGAATGGGAGAAAT+GGG + tig0025415:67257-67276 Msa1422550:CDS 35.0%
GATGTCAAAGGCTTTCATTT+AGG + tig0025415:67312-67331 Msa1422550:CDS 35.0%
AATTGAACTACATCAAGGTC+AGG - tig0025415:67375-67394 None:intergenic 35.0%
AGAAGCTAGTGATGAAATGA+AGG + tig0025415:67443-67462 Msa1422550:CDS 35.0%
GAAGCTAGTGATGAAATGAA+GGG + tig0025415:67444-67463 Msa1422550:CDS 35.0%
! AATATCCATCTCTTTTGTGC+TGG - tig0025415:67590-67609 None:intergenic 35.0%
TGTATGAAGTTTGAGCATGT+AGG + tig0025415:67696-67715 Msa1422550:CDS 35.0%
CTCTTCAAGATATATCGAGA+TGG - tig0025415:67777-67796 None:intergenic 35.0%
CTCGATATATCTTGAAGAGA+TGG + tig0025415:67778-67797 Msa1422550:CDS 35.0%
GATTATAATTCACCGTTGGT+TGG - tig0025415:68175-68194 None:intergenic 35.0%
CAGACAACAGTATTTGAAGA+AGG - tig0025415:68230-68249 None:intergenic 35.0%
AGATGAAACATATGGAGATG+GGG - tig0025415:68264-68283 None:intergenic 35.0%
GAGATGAAACATATGGAGAT+GGG - tig0025415:68265-68284 None:intergenic 35.0%
TGAGATGAAACATATGGAGA+TGG - tig0025415:68266-68285 None:intergenic 35.0%
GATAAAAGCTTACCTGCAAA+GGG - tig0025415:68298-68317 None:intergenic 35.0%
TGATAAAAGCTTACCTGCAA+AGG - tig0025415:68299-68318 None:intergenic 35.0%
!!! TGGTTTTGAAACCAATGAAG+TGG + tig0025415:68527-68546 Msa1422550:CDS 35.0%
TGATGATCTAACACAAGATC+AGG - tig0025415:68573-68592 None:intergenic 35.0%
TGGTTAGAATAGAAGTTTGG+CGG - tig0025415:68618-68637 None:intergenic 35.0%
TGTTGCATCTGATTATTGCT+TGG - tig0025415:68793-68812 None:intergenic 35.0%
AAGCAATAATCAGATGCAAC+AGG + tig0025415:68792-68811 Msa1422550:CDS 35.0%
!! GCTTTGTGTATATTTGCTGT+AGG + tig0025415:68909-68928 Msa1422550:intron 35.0%
!! TGTATATTTGCTGTAGGGTA+TGG + tig0025415:68915-68934 Msa1422550:intron 35.0%
! AGGATTCTGAATGAGGATTT+TGG - tig0025415:68951-68970 None:intergenic 35.0%
AATAATGGCATTGCCAATAC+AGG - tig0025415:69050-69069 None:intergenic 35.0%
ATATTAAAAGATACCCCGTC+CGG + tig0025415:69178-69197 Msa1422550:three_prime_UTR 35.0%
AATGGCTCTCAAACTCAATC+TGG + tig0025415:66073-66092 Msa1422550:CDS 40.0%
! TCTGGTAATGCATCAGCAAA+TGG + tig0025415:66091-66110 Msa1422550:CDS 40.0%
ACAAGTATGCCAGATTGATG+GGG + tig0025415:66185-66204 Msa1422550:CDS 40.0%
AACATTGAACCCCATCAATC+TGG - tig0025415:66197-66216 None:intergenic 40.0%
TAGGAGTAAGGTACATGGTA+TGG + tig0025415:66234-66253 Msa1422550:CDS 40.0%
TACATGGTATGGTGGTTTCT+AGG + tig0025415:66245-66264 Msa1422550:CDS 40.0%
AAATTTACCTGCTCCAGAGA+GGG + tig0025415:66268-66287 Msa1422550:CDS 40.0%
TACTGTGAAGAAACATCGGA+AGG + tig0025415:66318-66337 Msa1422550:CDS 40.0%
!! ATTTTGTGTTGCTCAAGCTG+TGG + tig0025415:66486-66505 Msa1422550:CDS 40.0%
TTCTGTGAGGGAAAGAGATA+TGG + tig0025415:66630-66649 Msa1422550:intron 40.0%
TCTGTGAGGGAAAGAGATAT+GGG + tig0025415:66631-66650 Msa1422550:intron 40.0%
AGGGAAAGAGATATGGGTAA+AGG + tig0025415:66637-66656 Msa1422550:intron 40.0%
TTTCTGGGCTGATGACAATA+TGG + tig0025415:66762-66781 Msa1422550:intron 40.0%
! GTGGTAGATTACGACCATTT+TGG + tig0025415:66784-66803 Msa1422550:intron 40.0%
! GGTAGATTACGACCATTTTG+GGG + tig0025415:66786-66805 Msa1422550:intron 40.0%
! CGGCCATTTGTACAGTTTTT+AGG + tig0025415:66853-66872 Msa1422550:intron 40.0%
! TGGCTGTTTCGAACTTTCAT+TGG + tig0025415:66949-66968 Msa1422550:intron 40.0%
AATGTCTGGGAAAAAGCCTA+AGG + tig0025415:66975-66994 Msa1422550:intron 40.0%
!! TGTTCTGTGATAATGGCCTT+AGG - tig0025415:66994-67013 None:intergenic 40.0%
!! TGGTTTTACCTGAAACAGAC+CGG + tig0025415:67043-67062 Msa1422550:intron 40.0%
TCTGCCAAACACATGTATAC+CGG - tig0025415:67065-67084 None:intergenic 40.0%
GAGTTGTATATATGACCCCA+AGG + tig0025415:67155-67174 Msa1422550:intron 40.0%
TAAATTCTTCCTCGTCCTTG+GGG - tig0025415:67173-67192 None:intergenic 40.0%
GTAAATTCTTCCTCGTCCTT+GGG - tig0025415:67174-67193 None:intergenic 40.0%
AGTAAATTCTTCCTCGTCCT+TGG - tig0025415:67175-67194 None:intergenic 40.0%
AGAATTTACTCATGCTTGGG+GGG + tig0025415:67185-67204 Msa1422550:intron 40.0%
ATCTCAACCATTCATTTCGC+TGG - tig0025415:67233-67252 None:intergenic 40.0%
AGCGAAATGAATGGTTGAGA+TGG + tig0025415:67232-67251 Msa1422550:intron 40.0%
CCTCCACTATCCTTTCAAAA+TGG - tig0025415:67404-67423 None:intergenic 40.0%
!! CCATTTTGAAAGGATAGTGG+AGG + tig0025415:67401-67420 Msa1422550:CDS 40.0%
GGATGTCTTCCAAAGCTAAT+GGG + tig0025415:67465-67484 Msa1422550:CDS 40.0%
! TCCAAAGCTAATGGGTAATG+TGG + tig0025415:67473-67492 Msa1422550:CDS 40.0%
! ACCACATTACCCATTAGCTT+TGG - tig0025415:67477-67496 None:intergenic 40.0%
AGAGATGGACAAAAGATGCA+AGG + tig0025415:67793-67812 Msa1422550:CDS 40.0%
ATGGACAAAAGATGCAAGGT+TGG + tig0025415:67797-67816 Msa1422550:CDS 40.0%
TGGACAAAAGATGCAAGGTT+GGG + tig0025415:67798-67817 Msa1422550:CDS 40.0%
ATGCAAGGTTGGGTAATTTC+AGG + tig0025415:67808-67827 Msa1422550:CDS 40.0%
TGCAAGGTTGGGTAATTTCA+GGG + tig0025415:67809-67828 Msa1422550:CDS 40.0%
!! AGCTTGCTACAACCATCTTA+GGG - tig0025415:67863-67882 None:intergenic 40.0%
CCACAGACTTGTCAAGTTAT+CGG + tig0025415:67899-67918 Msa1422550:CDS 40.0%
CAACTTGACGAAGTTATGGA+AGG + tig0025415:67966-67985 Msa1422550:CDS 40.0%
GAGTAAGCGAAGAAATTGAC+AGG + tig0025415:68129-68148 Msa1422550:CDS 40.0%
ATGAAACATATGGAGATGGG+GGG - tig0025415:68262-68281 None:intergenic 40.0%
GATGAAACATATGGAGATGG+GGG - tig0025415:68263-68282 None:intergenic 40.0%
CATGCATTGAACCACTTCAT+TGG - tig0025415:68541-68560 None:intergenic 40.0%
TAGAATAGAAGTTTGGCGGT+TGG - tig0025415:68614-68633 None:intergenic 40.0%
CATCTGATTATTGCTTGGCA+CGG - tig0025415:68788-68807 None:intergenic 40.0%
CGATAACCCACCAAAAGTTT+GGG - tig0025415:68859-68878 None:intergenic 40.0%
!!! CCAAACTTTTGGTGGGTTAT+CGG + tig0025415:68857-68876 Msa1422550:intron 40.0%
!!! CAAACTTTTGGTGGGTTATC+GGG + tig0025415:68858-68877 Msa1422550:intron 40.0%
ATGGTCACACAGCAAAATAC+AGG - tig0025415:68971-68990 None:intergenic 40.0%
AGTCAGAACTTCGGTATCTA+TGG - tig0025415:68990-69009 None:intergenic 40.0%
GTTATGCAGAGTCAGAACTT+CGG - tig0025415:68999-69018 None:intergenic 40.0%
CATAACGTATCAGAGGATCA+TGG + tig0025415:69013-69032 Msa1422550:exon 40.0%
AAATACACTTAAGACCGGAC+GGG - tig0025415:69195-69214 None:intergenic 40.0%
CAAATACACTTAAGACCGGA+CGG - tig0025415:69196-69215 None:intergenic 40.0%
! CGTTCGGACTGCAACAATTT+TGG - tig0025415:66042-66061 None:intergenic 45.0%
CCATTAGCGTGAAGAACGTT+CGG - tig0025415:66058-66077 None:intergenic 45.0%
GGTAATGCATCAGCAAATGG+AGG + tig0025415:66094-66113 Msa1422550:CDS 45.0%
! GGCTTTGTCAACACAGTGTT+CGG + tig0025415:66115-66134 Msa1422550:CDS 45.0%
GTCAACACAGTGTTCGGAAT+CGG + tig0025415:66121-66140 Msa1422550:CDS 45.0%
GAGTAAGGTACATGGTATGG+TGG + tig0025415:66237-66256 Msa1422550:CDS 45.0%
GAAATTTACCTGCTCCAGAG+AGG + tig0025415:66267-66286 Msa1422550:CDS 45.0%
TTTCCTACGATATCCCTCTC+TGG - tig0025415:66284-66303 None:intergenic 45.0%
GTGCTTCAAAATGGGTAACC+TGG - tig0025415:66402-66421 None:intergenic 45.0%
GATATGGGTAAAGGAGAAGC+TGG + tig0025415:66646-66665 Msa1422550:intron 45.0%
GTCATCAACATCGAGCTGTA+TGG - tig0025415:66728-66747 None:intergenic 45.0%
! GCAGAACAGACAGAGCTTTT+CGG + tig0025415:66833-66852 Msa1422550:intron 45.0%
ACTTTCATTGGCGCAATGTC+TGG + tig0025415:66961-66980 Msa1422550:intron 45.0%
CTTTCATTGGCGCAATGTCT+GGG + tig0025415:66962-66981 Msa1422550:intron 45.0%
CATGTATACCGGTCTGTTTC+AGG - tig0025415:67054-67073 None:intergenic 45.0%
CAGACCGGTATACATGTGTT+TGG + tig0025415:67058-67077 Msa1422550:intron 45.0%
TATATATGACCCCAAGGACG+AGG + tig0025415:67161-67180 Msa1422550:intron 45.0%
GGGATGTCTTCCAAAGCTAA+TGG + tig0025415:67464-67483 Msa1422550:CDS 45.0%
GTCAACCAGCACAAAAGAGA+TGG + tig0025415:67582-67601 Msa1422550:CDS 45.0%
!! CAGCTTGCTACAACCATCTT+AGG - tig0025415:67864-67883 None:intergenic 45.0%
CCGATAACTTGACAAGTCTG+TGG - tig0025415:67902-67921 None:intergenic 45.0%
AAGAGCTTCTGAATCTGTGG+AGG + tig0025415:67923-67942 Msa1422550:CDS 45.0%
AGAGCTTCTGAATCTGTGGA+GGG + tig0025415:67924-67943 Msa1422550:CDS 45.0%
!! AGGGTTATCAGTTTGCTGTG+AGG + tig0025415:67943-67962 Msa1422550:CDS 45.0%
GAGGCAACTTGACGAAGTTA+TGG + tig0025415:67962-67981 Msa1422550:CDS 45.0%
TCAACCAAATCTCCCTTTGC+AGG + tig0025415:68283-68302 Msa1422550:CDS 45.0%
CTTACCTGCAAAGGGAGATT+TGG - tig0025415:68290-68309 None:intergenic 45.0%
AAAGGCAAGGAGAACTCTGT+TGG - tig0025415:68638-68657 None:intergenic 45.0%
TGCGATAGTTGAGAAAGGCA+AGG - tig0025415:68651-68670 None:intergenic 45.0%
TTGCCTTTCTCAACTATCGC+AGG + tig0025415:68650-68669 Msa1422550:CDS 45.0%
ACACCTGCGATAGTTGAGAA+AGG - tig0025415:68656-68675 None:intergenic 45.0%
! CGGACTCATGGTATGTGTTT+TGG - tig0025415:68768-68787 None:intergenic 45.0%
! AAACCCCCTTCCCAAACTTT+TGG + tig0025415:68846-68865 Msa1422550:intron 45.0%
ACCCACCAAAAGTTTGGGAA+GGG - tig0025415:68854-68873 None:intergenic 45.0%
AACCCACCAAAAGTTTGGGA+AGG - tig0025415:68855-68874 None:intergenic 45.0%
CCGATAACCCACCAAAAGTT+TGG - tig0025415:68860-68879 None:intergenic 45.0%
GACTCTGCATAACGTATCAG+AGG + tig0025415:69006-69025 Msa1422550:exon 45.0%
GGTCGAACCATATCCTGTAT+TGG + tig0025415:69034-69053 Msa1422550:three_prime_UTR 45.0%
GGCATTGCCAATACAGGATA+TGG - tig0025415:69044-69063 None:intergenic 45.0%
AATACACTTAAGACCGGACG+GGG - tig0025415:69194-69213 None:intergenic 45.0%
CCGAACGTTCTTCACGCTAA+TGG + tig0025415:66055-66074 Msa1422550:CDS 50.0%
CACAGTGTTCGGAATCGGTA+CGG + tig0025415:66126-66145 Msa1422550:CDS 50.0%
GGAGACGAGAACTGGATGTT+TGG + tig0025415:66339-66358 Msa1422550:CDS 50.0%
! TTGTGTTGCTCAAGCTGTGG+AGG + tig0025415:66489-66508 Msa1422550:CDS 50.0%
AGGAGAAGCTGGACGTTTGA+TGG + tig0025415:66657-66676 Msa1422550:intron 50.0%
GGAGAAGCTGGACGTTTGAT+GGG + tig0025415:66658-66677 Msa1422550:intron 50.0%
CTGGGCTGATGACAATATGG+TGG + tig0025415:66765-66784 Msa1422550:intron 50.0%
!! GAGAAATGGGCTGTCTGCTT+TGG + tig0025415:67270-67289 Msa1422550:CDS 50.0%
GGCAAGAGCTTCTGAATCTG+TGG + tig0025415:67920-67939 Msa1422550:CDS 50.0%
TGCTTCAGAAAGTGAGCCTG+CGG + tig0025415:68058-68077 Msa1422550:CDS 50.0%
TCCTCAGTCGCATGTCCATT+CGG - tig0025415:68092-68111 None:intergenic 50.0%
AATTGACAGGACCGCTACAC+TGG + tig0025415:68142-68161 Msa1422550:CDS 50.0%
TTGGCACCTATCCAGTGTAG+CGG - tig0025415:68156-68175 None:intergenic 50.0%
ACTGGATAGGTGCCAACCAA+CGG + tig0025415:68160-68179 Msa1422550:CDS 50.0%
AGAAGTTTGGCGGTTGGAAC+AGG - tig0025415:68608-68627 None:intergenic 50.0%
TATTGCTTGGCACGGACTCA+TGG - tig0025415:68780-68799 None:intergenic 50.0%
CCACCAAAAGTTTGGGAAGG+GGG - tig0025415:68852-68871 None:intergenic 50.0%
CCCACCAAAAGTTTGGGAAG+GGG - tig0025415:68853-68872 None:intergenic 50.0%
! CCCCTTCCCAAACTTTTGGT+GGG + tig0025415:68850-68869 Msa1422550:intron 50.0%
!! GGTGGGTTATCGGGTGTTTA+TGG + tig0025415:68867-68886 Msa1422550:intron 50.0%
GGGTTATCGGGTGTTTATGG+TGG + tig0025415:68870-68889 Msa1422550:intron 50.0%
!! ACGATATCCCTCTCTGGAGC+AGG - tig0025415:66278-66297 None:intergenic 55.0%
GCTCCAGAGAGGGATATCGT+AGG + tig0025415:66278-66297 Msa1422550:CDS 55.0%
CATCGGAAGGAGACGAGAAC+TGG + tig0025415:66331-66350 Msa1422550:CDS 55.0%
TCATGCTTGGGGGGTTATGC+TGG + tig0025415:67194-67213 Msa1422550:intron 55.0%
CCATTCGGAAAGATCACCGC+AGG - tig0025415:68077-68096 None:intergenic 55.0%
CCTGCGGTGATCTTTCCGAA+TGG + tig0025415:68074-68093 Msa1422550:CDS 55.0%
TCCGAATGGACATGCGACTG+AGG + tig0025415:68088-68107 Msa1422550:CDS 55.0%
TGGACATGCGACTGAGGATC+AGG + tig0025415:68094-68113 Msa1422550:CDS 55.0%
ACAGGACCGCTACACTGGAT+AGG + tig0025415:68147-68166 Msa1422550:CDS 55.0%
! CCCCCTTCCCAAACTTTTGG+TGG + tig0025415:68849-68868 Msa1422550:intron 55.0%
Chromosome Type Strat End Strand Name
tig0025415 gene 65947 69266 65947 ID=Msa1422550;Name=Msa1422550
tig0025415 mRNA 65947 69266 65947 ID=Msa1422550-mRNA-1;Parent=Msa1422550;Name=Msa1422550-mRNA-1;_AED=0.15;_eAED=0.25;_QI=0|0.75|0.6|1|1|1|5|249|644
tig0025415 exon 65947 66510 65947 ID=Msa1422550-mRNA-1:exon:2649;Parent=Msa1422550-mRNA-1
tig0025415 exon 67234 68304 67234 ID=Msa1422550-mRNA-1:exon:2650;Parent=Msa1422550-mRNA-1
tig0025415 exon 68519 68671 68519 ID=Msa1422550-mRNA-1:exon:2651;Parent=Msa1422550-mRNA-1
tig0025415 exon 68754 68813 68754 ID=Msa1422550-mRNA-1:exon:2652;Parent=Msa1422550-mRNA-1
tig0025415 exon 68931 69266 68931 ID=Msa1422550-mRNA-1:exon:2653;Parent=Msa1422550-mRNA-1
tig0025415 CDS 65947 66510 65947 ID=Msa1422550-mRNA-1:cds;Parent=Msa1422550-mRNA-1
tig0025415 CDS 67234 68304 67234 ID=Msa1422550-mRNA-1:cds;Parent=Msa1422550-mRNA-1
tig0025415 CDS 68519 68671 68519 ID=Msa1422550-mRNA-1:cds;Parent=Msa1422550-mRNA-1
tig0025415 CDS 68754 68813 68754 ID=Msa1422550-mRNA-1:cds;Parent=Msa1422550-mRNA-1
tig0025415 CDS 68931 69017 68931 ID=Msa1422550-mRNA-1:cds;Parent=Msa1422550-mRNA-1
tig0025415 three_prime_UTR 69018 69266 69018 ID=Msa1422550-mRNA-1:three_prime_utr;Parent=Msa1422550-mRNA-1
Gene Sequence

>Msa1422550

ATGGATGGTGATCATAACAACTTAGGTAATGTGCATGTAGAAAATGTTGATGTTGGACTTGGAACATCCAATAGTTTGGACTTGAATGTGGACCAAAATTGTTGCAGTCCGAACGTTCTTCACGCTAATGGCTCTCAAACTCAATCTGGTAATGCATCAGCAAATGGAGGCTTTGTCAACACAGTGTTCGGAATCGGTACGGTATTTGAGTCTGATGAACATGCATATAGATTCTATAACAAGTATGCCAGATTGATGGGGTTCAATGTTAGAAAAGATTGGATTAATAGGAGTAAGGTACATGGTATGGTGGTTTCTAGGAAATTTACCTGCTCCAGAGAGGGATATCGTAGGAAAGACGAGAGAGATTTTACTGTGAAGAAACATCGGAAGGAGACGAGAACTGGATGTTTGGCACACATGATCATTACTCGTCAACAAGATGGAAAATACCAGGTTACCCATTTTGAAGCACAACATAATCATGATGATATAAACCCAATCAATTCTAACATGTTGTTACTAGAGTTGCAAAATGAATTTTGTGTTGCTCAAGCTGTGGAGCGAAATGAATGGTTGAGATGGATGTATAGAGAATGGGAGAAATGGGCTGTCTGCTTTGGTCAGAATAGATTTTTTGTAGATGTCAAAGGCTTTCATTTAGGTGAGATGTTGTCTCACAAGCTTAGAAGCTATCTAAATCCTGACCTTGATGTAGTTCAATTTCTTAACCATTTTGAAAGGATAGTGGAGGAGCAACGATATAAAGAAATAGAAGCTAGTGATGAAATGAAGGGATGTCTTCCAAAGCTAATGGGTAATGTGGTTGTGTTGAAACATGCTAGTGTTGCTTATACTCCAAGAGCATTTGAAATATTTCAGCAACGATATGAGAACTCTTTGAATGTTATTGTCAACCAGCACAAAAGAGATGGATATTTGTTTGAGTATAAAGTGAATACATTTGGACATGCTAGACAGTACACTGTCACCTTCAATTCTTCAGATAATACAGTTGTCTGCAGTTGTATGAAGTTTGAGCATGTAGGTTTTCTTTGTAGCCATGCACTAAAAGTTCTTGATAATAGAAACATAAAAGTAGTTCCATCTCGATATATCTTGAAGAGATGGACAAAAGATGCAAGGTTGGGTAATTTCAGGGAGATTAAGCAATTTAAAATTCAAGATAACCCTAAGATGGTTGTAGCAAGCTGTTACAAAGATTTATGCCACAGACTTGTCAAGTTATCGGCAAGAGCTTCTGAATCTGTGGAGGGTTATCAGTTTGCTGTGAGGCAACTTGACGAAGTTATGGAAGGAGTGCAGAAAATCTTGATATTGAAAAGTGAGGAAGCTCAAGTTATCACCTCAAATAGTATTCATGTAGATGCTTCAGAAAGTGAGCCTGCGGTGATCTTTCCGAATGGACATGCGACTGAGGATCAGGATGAAAGTAACAGAGTAAGCGAAGAAATTGACAGGACCGCTACACTGGATAGGTGCCAACCAACGGTGAATTATAATCAAACTGATTCAGATAGGATTTTAAATGTAGAACCTTCTTCAAATACTGTTGTCTGCATTTCATGCCCCCCATCTCCATATGTTTCATCTCAACCAAATCTCCCTTTGCAGGGCTTATTTGGTTTTGAAACCAATGAAGTGGTTCAATGCATGTATGAACAGCCTGATCTTGTGTTAGATCATCAATCTAATACTAACCTGTTCCAACCGCCAAACTTCTATTCTAACCAACAGAGTTCTCCTTGCCTTTCTCAACTATCGCAGGAGCCTATAATCCAAAACACATACCATGAGTCCGTGCCAAGCAATAATCAGATGCAACAGGGTATGGATCTTGATATCCAAAATCCTCATTCAGAATCCTGTATTTTGCTGTGTGACCATAGATACCGAAGTTCTGACTCTGCATAA
Protein sequence

>Msa1422550

MDGDHNNLGNVHVENVDVGLGTSNSLDLNVDQNCCSPNVLHANGSQTQSGNASANGGFVNTVFGIGTVFESDEHAYRFYNKYARLMGFNVRKDWINRSKVHGMVVSRKFTCSREGYRRKDERDFTVKKHRKETRTGCLAHMIITRQQDGKYQVTHFEAQHNHDDINPINSNMLLLELQNEFCVAQAVERNEWLRWMYREWEKWAVCFGQNRFFVDVKGFHLGEMLSHKLRSYLNPDLDVVQFLNHFERIVEEQRYKEIEASDEMKGCLPKLMGNVVVLKHASVAYTPRAFEIFQQRYENSLNVIVNQHKRDGYLFEYKVNTFGHARQYTVTFNSSDNTVVCSCMKFEHVGFLCSHALKVLDNRNIKVVPSRYILKRWTKDARLGNFREIKQFKIQDNPKMVVASCYKDLCHRLVKLSARASESVEGYQFAVRQLDEVMEGVQKILILKSEEAQVITSNSIHVDASESEPAVIFPNGHATEDQDESNRVSEEIDRTATLDRCQPTVNYNQTDSDRILNVEPSSNTVVCISCPPSPYVSSQPNLPLQGLFGFETNEVVQCMYEQPDLVLDHQSNTNLFQPPNFYSNQQSSPCLSQLSQEPIIQNTYHESVPSNNQMQQGMDLDIQNPHSESCILLCDHRYRSSDSA*