AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1423140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1423140 MtrunA17_Chr1g0203721 94.541 458 23 1 187 644 428 883 0.0 874
Msa1423140 MtrunA17_Chr1g0203721 93.229 192 13 0 1 192 1 192 1.53e-121 382
Msa1423140 MtrunA17_Chr6g0458391 47.938 194 101 0 190 383 573 766 4.56e-56 203
Msa1423140 MtrunA17_Chr6g0458391 51.562 128 60 1 59 186 221 346 2.70e-34 139
Msa1423140 MtrunA17_Chr6g0458391 52.756 127 55 3 65 190 93 215 2.42e-32 133
Msa1423140 MtrunA17_Chr3g0082121 40.299 268 151 3 179 445 390 649 1.00e-42 164
Msa1423140 MtrunA17_Chr3g0082121 52.632 95 44 1 69 162 49 143 2.32e-23 105
Msa1423140 MtrunA17_Chr2g0324011 32.857 210 140 1 192 400 377 586 4.58e-34 137
Msa1423140 MtrunA17_Chr5g0448511 25.153 326 232 4 180 499 189 508 4.41e-27 115
Msa1423140 MtrunA17_Chr5g0448761 26.102 295 201 4 190 476 432 717 3.47e-26 114
Msa1423140 MtrunA17_Chr5g0448761 37.736 106 62 2 66 167 63 168 4.30e-12 69.3
Msa1423140 MtrunA17_Chr3g0118611 27.536 276 186 6 187 458 307 572 6.97e-25 110
Msa1423140 MtrunA17_Chr5g0401121 49.474 95 47 1 69 162 62 156 3.05e-24 99.8
Msa1423140 MtrunA17_Chr8g0363841 31.220 205 128 4 186 382 378 577 1.25e-23 105
Msa1423140 MtrunA17_Chr5g0440451 27.206 272 184 5 191 458 414 675 8.34e-23 103
Msa1423140 MtrunA17_Chr2g0329031 26.772 254 170 5 191 439 371 613 9.69e-22 100
Msa1423140 MtrunA17_Chr2g0329031 40.000 95 54 2 69 163 20 111 1.17e-11 68.2
Msa1423140 MtrunA17_Chr1g0202341 25.368 272 199 3 186 454 412 682 1.42e-21 99.4
Msa1423140 MtrunA17_Chr1g0207551 28.916 249 170 3 192 439 399 641 2.01e-21 99.4
Msa1423140 MtrunA17_Chr4g0033611 35.032 157 90 5 27 180 47 194 4.86e-19 87.4
Msa1423140 MtrunA17_Chr3g0080351 26.190 252 179 2 187 437 428 673 7.94e-19 91.3
Msa1423140 MtrunA17_Chr3g0080351 36.036 111 58 1 66 163 79 189 1.20e-12 71.2
Msa1423140 MtrunA17_Chr2g0329421 37.755 98 60 1 65 162 29 125 2.22e-16 78.6
Msa1423140 MtrunA17_Chr2g0287061 26.538 260 183 3 179 436 499 752 4.70e-16 82.0
Msa1423140 MtrunA17_Chr2g0287061 36.364 99 50 1 65 163 185 270 1.55e-12 70.9
Msa1423140 MtrunA17_Chr2g0287061 37.374 99 49 1 65 163 31 116 7.36e-12 68.6
Msa1423140 MtrunA17_Chr2g0291581 27.451 204 140 3 187 382 418 621 1.46e-15 80.5
Msa1423140 MtrunA17_Chr5g0441581 22.593 270 194 7 187 445 382 647 1.93e-15 80.1
Msa1423140 MtrunA17_Chr7g0233911 31.343 134 78 2 65 184 36 169 7.25e-15 74.3
Msa1423140 MtrunA17_Chr1g0198761 22.381 210 142 4 181 383 418 613 3.01e-13 73.2
Msa1423140 MtrunA17_Chr2g0308841 21.698 212 164 2 176 385 19 230 4.61e-13 69.7
Msa1423140 MtrunA17_Chr7g0238821 36.111 108 66 3 69 175 3 108 1.63e-12 64.3
Msa1423140 MtrunA17_Chr2g0329361 36.111 108 61 2 65 167 63 167 2.97e-12 67.4
Msa1423140 MtrunA17_Chr7g0258861 33.636 110 71 2 65 173 12 120 3.08e-12 68.2
Msa1423140 MtrunA17_Chr3g0078041 37.755 98 60 1 66 162 53 150 8.24e-12 65.5
Msa1423140 MtrunA17_Chr2g0287651 22.791 215 159 4 176 386 424 635 1.09e-11 68.2
Msa1423140 MtrunA17_Chr2g0287651 34.314 102 63 3 69 167 81 181 9.43e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1423140 AT4G38170.1 25.597 293 195 3 187 478 203 473 9.76e-29 121
Msa1423140 AT3G22170.2 26.471 272 194 2 186 457 422 687 4.86e-26 114
Msa1423140 AT3G22170.2 28.415 183 95 4 2 163 16 183 5.94e-12 69.3
Msa1423140 AT3G22170.1 26.471 272 194 2 186 457 422 687 4.86e-26 114
Msa1423140 AT3G22170.1 28.415 183 95 4 2 163 16 183 5.94e-12 69.3
Msa1423140 AT2G32250.2 28.214 280 187 6 190 463 361 632 1.62e-25 112
Msa1423140 AT2G32250.2 37.755 98 58 1 66 163 41 135 2.06e-11 67.8
Msa1423140 AT2G32250.4 28.214 280 187 6 190 463 361 632 1.62e-25 112
Msa1423140 AT2G32250.4 37.755 98 58 1 66 163 41 135 2.06e-11 67.8
Msa1423140 AT2G32250.6 28.214 280 187 6 190 463 361 632 1.74e-25 112
Msa1423140 AT2G32250.6 37.755 98 58 1 66 163 41 135 2.08e-11 67.8
Msa1423140 AT2G32250.1 28.214 280 187 6 190 463 361 632 1.74e-25 112
Msa1423140 AT2G32250.1 37.755 98 58 1 66 163 41 135 2.08e-11 67.8
Msa1423140 AT2G32250.3 28.214 280 187 6 190 463 361 632 1.74e-25 112
Msa1423140 AT2G32250.3 37.755 98 58 1 66 163 41 135 2.08e-11 67.8
Msa1423140 AT2G32250.8 28.214 280 187 6 190 463 320 591 2.33e-25 112
Msa1423140 AT2G32250.8 37.895 95 56 1 69 163 3 94 5.04e-11 66.6
Msa1423140 AT2G32250.7 28.214 280 187 6 190 463 320 591 2.36e-25 112
Msa1423140 AT2G32250.7 37.895 95 56 1 69 163 3 94 5.14e-11 66.2
Msa1423140 AT2G32250.5 28.214 280 187 6 190 463 361 632 2.39e-25 112
Msa1423140 AT2G32250.5 37.755 98 58 1 66 163 41 135 1.84e-11 67.8
Msa1423140 AT4G38180.1 30.412 194 134 1 190 382 441 634 1.05e-24 110
Msa1423140 AT4G38180.1 33.143 175 97 3 13 167 6 180 7.30e-16 82.0
Msa1423140 AT4G15090.1 23.195 457 289 11 186 636 413 813 6.91e-24 107
Msa1423140 AT4G15090.5 23.195 457 289 11 186 636 413 813 6.91e-24 107
Msa1423140 AT4G15090.3 23.195 457 289 11 186 636 413 813 6.91e-24 107
Msa1423140 AT4G15090.2 23.195 457 289 11 186 636 413 813 6.91e-24 107
Msa1423140 AT4G15090.4 23.195 457 289 11 186 636 413 813 6.91e-24 107
Msa1423140 AT2G27110.2 27.586 261 180 5 189 448 387 639 9.35e-22 100
Msa1423140 AT2G27110.1 27.586 261 180 5 189 448 387 639 9.35e-22 100
Msa1423140 AT2G27110.3 27.586 261 180 5 189 448 242 494 1.40e-21 100
Msa1423140 AT3G06250.2 28.405 257 172 5 190 444 514 760 5.60e-21 98.2
Msa1423140 AT3G06250.2 34.783 115 61 2 66 180 193 293 2.33e-12 70.9
Msa1423140 AT3G06250.2 35.354 99 51 1 65 163 30 115 4.40e-12 69.7
Msa1423140 AT3G06250.1 28.405 257 172 5 190 444 514 760 5.60e-21 98.2
Msa1423140 AT3G06250.1 34.783 115 61 2 66 180 193 293 2.33e-12 70.9
Msa1423140 AT3G06250.1 35.354 99 51 1 65 163 30 115 4.40e-12 69.7
Msa1423140 AT3G06250.3 28.405 257 172 5 190 444 514 760 5.60e-21 98.2
Msa1423140 AT3G06250.3 34.783 115 61 2 66 180 193 293 2.33e-12 70.9
Msa1423140 AT3G06250.3 35.354 99 51 1 65 163 30 115 4.40e-12 69.7
Msa1423140 AT5G18960.2 27.027 259 169 6 192 444 540 784 4.48e-20 95.5
Msa1423140 AT5G18960.2 30.464 151 84 3 13 163 1 130 1.34e-12 71.6
Msa1423140 AT5G18960.2 38.679 106 51 2 66 171 214 305 1.36e-12 71.6
Msa1423140 AT5G18960.1 27.027 259 169 6 192 444 540 784 4.48e-20 95.5
Msa1423140 AT5G18960.1 30.464 151 84 3 13 163 1 130 1.34e-12 71.6
Msa1423140 AT5G18960.1 38.679 106 51 2 66 171 214 305 1.36e-12 71.6
Msa1423140 AT1G76320.2 25.200 250 176 3 192 439 356 596 1.68e-19 93.6
Msa1423140 AT1G76320.1 25.200 250 176 3 192 439 356 596 1.82e-19 93.6
Msa1423140 AT1G76320.4 25.200 250 176 3 192 439 356 596 1.82e-19 93.6
Msa1423140 AT1G76320.3 25.200 250 176 3 192 439 356 596 4.65e-19 92.0
Msa1423140 AT3G07500.1 42.593 108 61 1 65 172 35 141 1.90e-18 85.1
Msa1423140 AT4G19990.6 28.520 277 190 4 190 462 365 637 2.95e-18 89.7
Msa1423140 AT4G19990.2 28.520 277 190 4 190 462 365 637 2.95e-18 89.7
Msa1423140 AT1G52520.1 27.509 269 182 6 186 444 433 698 2.96e-18 89.4
Msa1423140 AT4G19990.5 28.520 277 190 4 190 462 365 637 3.51e-18 89.4
Msa1423140 AT4G19990.3 28.520 277 190 4 190 462 365 637 3.83e-18 89.4
Msa1423140 AT4G19990.4 28.520 277 190 4 190 462 365 637 3.83e-18 89.4
Msa1423140 AT4G19990.7 28.520 277 190 4 190 462 379 651 4.17e-18 89.0
Msa1423140 AT4G19990.1 28.520 277 190 4 190 462 308 580 4.26e-18 89.0
Msa1423140 AT3G59470.3 39.796 98 58 1 65 162 72 168 5.49e-18 84.7
Msa1423140 AT3G59470.1 39.796 98 58 1 65 162 72 168 5.49e-18 84.7
Msa1423140 AT3G59470.2 39.796 98 58 1 65 162 84 180 7.14e-18 84.3
Msa1423140 AT2G43280.1 36.735 98 61 1 66 162 25 122 2.53e-13 69.7
Msa1423140 AT4G12850.4 32.653 98 63 1 65 162 13 107 2.87e-13 67.8
Msa1423140 AT4G12850.2 32.653 98 63 1 65 162 13 107 6.80e-12 63.9
Msa1423140 AT4G12850.1 32.653 98 63 1 65 162 13 107 1.19e-11 64.3
Msa1423140 AT1G80010.1 24.762 210 151 4 180 382 452 661 3.98e-11 66.6

Find 145 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTCGGACTGCAACAATTT+TGG 0.148412 tig0025513:+27619 None:intergenic
AGGATTCTGAATGAGGATTT+TGG 0.176822 tig0025513:+24710 None:intergenic
TAATATGAATGATGAATATT+TGG 0.181624 tig0025513:+24497 None:intergenic
AAGTGTATTTGATCTAAATT+TGG 0.221536 tig0025513:-24453 Msa1423140:three_prime_UTR
CAGCTTGCTACAACCATCTT+AGG 0.224643 tig0025513:+25797 None:intergenic
CGGACTCATGGTATGTGTTT+TGG 0.233173 tig0025513:+24893 None:intergenic
GATTATGTTGTGCTTCAAAA+TGG 0.267872 tig0025513:+27250 None:intergenic
ATGCAAGGTTGGGTAATTTC+AGG 0.281403 tig0025513:-25850 Msa1423140:CDS
TTAGAAAAGATTGGATTAAT+AGG 0.287407 tig0025513:-27443 Msa1423140:CDS
GGCATTGCCAATACAGGATA+TGG 0.302964 tig0025513:+24617 None:intergenic
CTTACCTGCAAAGGGAGATT+TGG 0.310345 tig0025513:+25371 None:intergenic
CCTCCACTATCCTTTCAAAA+TGG 0.316329 tig0025513:+26257 None:intergenic
ACTTGGAACATCCAATAGTT+TGG 0.334138 tig0025513:-27655 Msa1423140:CDS
CACATTCAAGTCCAAACTAT+TGG 0.341769 tig0025513:+27644 None:intergenic
CATGCATTGAACCACTTCAT+TGG 0.347987 tig0025513:+25120 None:intergenic
CCGAACGTTCTTCACGCTAA+TGG 0.356305 tig0025513:-27603 Msa1423140:CDS
AACAACATGTTAGAATTGAT+TGG 0.361206 tig0025513:+27213 None:intergenic
TACATGGTATGGTGGTTTCT+AGG 0.366745 tig0025513:-27413 Msa1423140:CDS
TCAACCAAATCTCCCTTTGC+AGG 0.367806 tig0025513:-25375 Msa1423140:intron
GGCTTTGTCAACACAGTGTT+CGG 0.372860 tig0025513:-27543 Msa1423140:CDS
GGTTCAATGTTAGAAAAGAT+TGG 0.384400 tig0025513:-27452 Msa1423140:CDS
CCACAGACTTGTCAAGTTAT+CGG 0.386090 tig0025513:-25759 Msa1423140:CDS
GATGTCAAAGGCTTTCATTT+AGG 0.388977 tig0025513:-26346 Msa1423140:CDS
GTATAGAGAATGGGAGAAAT+GGG 0.389543 tig0025513:-26401 Msa1423140:CDS
GGGATGTCTTCCAAAGCTAA+TGG 0.397500 tig0025513:-26194 Msa1423140:CDS
TGAAATATTTCAAATGCTCT+TGG 0.400052 tig0025513:+26130 None:intergenic
TGTATAGAGAATGGGAGAAA+TGG 0.401167 tig0025513:-26402 Msa1423140:CDS
TTTGGTTGAGATGAAACATA+TGG 0.404147 tig0025513:+25389 None:intergenic
AGAAGTTTGGCGGTTGGAAC+AGG 0.408816 tig0025513:+25053 None:intergenic
TGTTGGTTAGAATAGAAGTT+TGG 0.411083 tig0025513:+25040 None:intergenic
GAGTATAAAGTGAATACATT+TGG 0.412707 tig0025513:-26043 Msa1423140:CDS
TGTATTATCTGAAGAATTGA+AGG 0.418492 tig0025513:+25997 None:intergenic
ACCACATTACCCATTAGCTT+TGG 0.425279 tig0025513:+26184 None:intergenic
AACATTGAACCCCATCAATC+TGG 0.425557 tig0025513:+27464 None:intergenic
ACAACATGTTAGAATTGATT+GGG 0.429453 tig0025513:+27214 None:intergenic
AGTCAGAACTTCGGTATCTA+TGG 0.430265 tig0025513:+24671 None:intergenic
TGGACATGCGACTGAGGATC+AGG 0.436653 tig0025513:-25564 Msa1423140:CDS
AATAATGGCATTGCCAATAC+AGG 0.438574 tig0025513:+24611 None:intergenic
GGAGACGAGAACTGGATGTT+TGG 0.438770 tig0025513:-27319 Msa1423140:CDS
CTCTTCCCTTTGTTTAAGAC+CGG 0.439983 tig0025513:-24414 Msa1423140:three_prime_UTR
CATCGGAAGGAGACGAGAAC+TGG 0.445746 tig0025513:-27327 Msa1423140:CDS
GAGAAATGGGCTGTCTGCTT+TGG 0.447459 tig0025513:-26388 Msa1423140:CDS
TAACAAGTATGCCAGATTGA+TGG 0.451658 tig0025513:-27475 Msa1423140:CDS
AATGGCTCTCAAACTCAATC+TGG 0.454597 tig0025513:-27585 Msa1423140:CDS
ATAATCAAACTGATTCAGAT+AGG 0.455395 tig0025513:-25469 Msa1423140:CDS
AACAAGTATGCCAGATTGAT+GGG 0.458593 tig0025513:-27474 Msa1423140:CDS
TGATGATCTAACACAAGATC+AGG 0.463119 tig0025513:+25088 None:intergenic
TCCTCAGTCGCATGTCCATT+CGG 0.464228 tig0025513:+25569 None:intergenic
GTCAACACAGTGTTCGGAAT+CGG 0.464277 tig0025513:-27537 Msa1423140:CDS
GGATGTCTTCCAAAGCTAAT+GGG 0.465360 tig0025513:-26193 Msa1423140:CDS
AGAAGCTAGTGATGAAATGA+AGG 0.466016 tig0025513:-26215 Msa1423140:CDS
ATCTACATGAATACTATTTG+AGG 0.467176 tig0025513:+25622 None:intergenic
GAGATGGATGTATAGAGAAT+GGG 0.471472 tig0025513:-26410 Msa1423140:CDS
GGTCGAACCATATCCTGTAT+TGG 0.475597 tig0025513:-24624 Msa1423140:three_prime_UTR
CTCCCTCCGGTCTTAAACAA+AGG 0.475629 tig0025513:+24408 None:intergenic
TGTTGCATCTGATTATTGCT+TGG 0.479286 tig0025513:+24868 None:intergenic
TGATAAAAGCTTACCTGCAA+AGG 0.481590 tig0025513:+25362 None:intergenic
TGCAAGGTTGGGTAATTTCA+GGG 0.481977 tig0025513:-25849 Msa1423140:CDS
CAGACAACAGTATTTGAAGA+AGG 0.483624 tig0025513:+25431 None:intergenic
AGCTTGCTACAACCATCTTA+GGG 0.484144 tig0025513:+25798 None:intergenic
GAGGCAACTTGACGAAGTTA+TGG 0.484472 tig0025513:-25696 Msa1423140:CDS
AATTGAACTACATCAAGGTC+AGG 0.492101 tig0025513:+26286 None:intergenic
AATTCAAGATAACCCTAAGA+TGG 0.492105 tig0025513:-25810 Msa1423140:CDS
CTCGATATATCTTGAAGAGA+TGG 0.495398 tig0025513:-25880 Msa1423140:CDS
AATTGACAGGACCGCTACAC+TGG 0.496426 tig0025513:-25516 Msa1423140:CDS
AAGCAATAATCAGATGCAAC+AGG 0.498238 tig0025513:-24866 Msa1423140:intron
ATGGTCACACAGCAAAATAC+AGG 0.502031 tig0025513:+24690 None:intergenic
TCTGGTAATGCATCAGCAAA+TGG 0.509074 tig0025513:-27567 Msa1423140:CDS
TAGGAGTAAGGTACATGGTA+TGG 0.509545 tig0025513:-27424 Msa1423140:CDS
ACGATATCCCTCTCTGGAGC+AGG 0.509622 tig0025513:+27383 None:intergenic
GATGGTGATCATAACAACTT+AGG 0.510043 tig0025513:-27708 Msa1423140:CDS
GATAAAAGCTTACCTGCAAA+GGG 0.510969 tig0025513:+25363 None:intergenic
CCATTAGCGTGAAGAACGTT+CGG 0.511332 tig0025513:+27603 None:intergenic
ATTATGTTGTGCTTCAAAAT+GGG 0.511977 tig0025513:+27251 None:intergenic
TGAGATGAAACATATGGAGA+TGG 0.517004 tig0025513:+25395 None:intergenic
GAGATGAAACATATGGAGAT+GGG 0.517572 tig0025513:+25396 None:intergenic
ATGGACAAAAGATGCAAGGT+TGG 0.517774 tig0025513:-25861 Msa1423140:CDS
TAGAATAGAAGTTTGGCGGT+TGG 0.518493 tig0025513:+25047 None:intergenic
TCCCTCCGGTCTTAAACAAA+GGG 0.519160 tig0025513:+24409 None:intergenic
AAAATACAGGATTCTGAATG+AGG 0.519697 tig0025513:+24703 None:intergenic
ATCATTACTCGTCAACAAGA+TGG 0.520020 tig0025513:-27288 Msa1423140:CDS
TGAGATGGATGTATAGAGAA+TGG 0.522848 tig0025513:-26411 Msa1423140:CDS
GAAAATGTTGATGTTGGACT+TGG 0.524439 tig0025513:-27672 Msa1423140:CDS
TTTCCTACGATATCCCTCTC+TGG 0.525239 tig0025513:+27377 None:intergenic
TATTGCTTGGCACGGACTCA+TGG 0.526252 tig0025513:+24881 None:intergenic
TGGTTAGAATAGAAGTTTGG+CGG 0.528921 tig0025513:+25043 None:intergenic
CATGTAGAAAATGTTGATGT+TGG 0.531700 tig0025513:-27678 Msa1423140:CDS
AGATTGGATTAATAGGAGTA+AGG 0.541743 tig0025513:-27436 Msa1423140:CDS
GATTATAATTCACCGTTGGT+TGG 0.543940 tig0025513:+25486 None:intergenic
CAATAGTTTGGACTTGAATG+TGG 0.544732 tig0025513:-27643 Msa1423140:CDS
TGGACAAAAGATGCAAGGTT+GGG 0.547513 tig0025513:-25860 Msa1423140:CDS
TTGCCTTTCTCAACTATCGC+AGG 0.548639 tig0025513:-25008 Msa1423140:intron
TTGTGTTGCTCAAGCTGTGG+AGG 0.559431 tig0025513:-27169 Msa1423140:intron
TTCCCTTTGTTTAAGACCGG+AGG 0.560342 tig0025513:-24411 None:intergenic
GTTTGATTATAATTCACCGT+TGG 0.560586 tig0025513:+25482 None:intergenic
ATAAACTCCAGCGAAATGAA+TGG 0.563219 tig0025513:-26435 Msa1423140:intron
GTCAACCAGCACAAAAGAGA+TGG 0.564837 tig0025513:-26076 Msa1423140:CDS
ATATTAAAAGATACCCCGTC+CGG 0.573728 tig0025513:-24480 Msa1423140:three_prime_UTR
AGATCAAATACACTTAAGAC+CGG 0.574251 tig0025513:+24461 None:intergenic
TAAGAAATTGAACTACATCA+AGG 0.574535 tig0025513:+26281 None:intergenic
AAAGGCAAGGAGAACTCTGT+TGG 0.577643 tig0025513:+25023 None:intergenic
CACAGTGTTCGGAATCGGTA+CGG 0.578865 tig0025513:-27532 Msa1423140:CDS
TCCAAAGCTAATGGGTAATG+TGG 0.580465 tig0025513:-26185 Msa1423140:CDS
AATCTTGATATTGAAAAGTG+AGG 0.582977 tig0025513:-25660 Msa1423140:CDS
ACAGGACCGCTACACTGGAT+AGG 0.587540 tig0025513:-25511 Msa1423140:CDS
AAATTTACCTGCTCCAGAGA+GGG 0.588352 tig0025513:-27390 Msa1423140:CDS
AAGAGCTTCTGAATCTGTGG+AGG 0.588474 tig0025513:-25735 Msa1423140:CDS
TACTGTGAAGAAACATCGGA+AGG 0.591189 tig0025513:-27340 Msa1423140:CDS
AGCGAAATGAATGGTTGAGA+TGG 0.591609 tig0025513:-26426 Msa1423140:CDS
AAATACACTTAAGACCGGAC+GGG 0.592534 tig0025513:+24466 None:intergenic
CCATTCGGAAAGATCACCGC+AGG 0.593497 tig0025513:+25584 None:intergenic
GAGTAAGCGAAGAAATTGAC+AGG 0.593964 tig0025513:-25529 Msa1423140:CDS
ACACCTGCGATAGTTGAGAA+AGG 0.595685 tig0025513:+25005 None:intergenic
GGCAAGAGCTTCTGAATCTG+TGG 0.597094 tig0025513:-25738 Msa1423140:CDS
GAGTAAGGTACATGGTATGG+TGG 0.599166 tig0025513:-27421 Msa1423140:CDS
CATCTGATTATTGCTTGGCA+CGG 0.603767 tig0025513:+24873 None:intergenic
CAAATACACTTAAGACCGGA+CGG 0.604119 tig0025513:+24465 None:intergenic
TCCGAATGGACATGCGACTG+AGG 0.607571 tig0025513:-25570 Msa1423140:CDS
ACTGGATAGGTGCCAACCAA+CGG 0.611215 tig0025513:-25498 Msa1423140:CDS
AGATGAAACATATGGAGATG+GGG 0.614637 tig0025513:+25397 None:intergenic
AATACACTTAAGACCGGACG+GGG 0.617779 tig0025513:+24467 None:intergenic
TGTATGAAGTTTGAGCATGT+AGG 0.618522 tig0025513:-25962 Msa1423140:CDS
CTCTTCAAGATATATCGAGA+TGG 0.620754 tig0025513:+25884 None:intergenic
TCAACAAGATGGAAAATACC+AGG 0.623065 tig0025513:-27277 Msa1423140:CDS
CCTGCGGTGATCTTTCCGAA+TGG 0.627373 tig0025513:-25584 Msa1423140:CDS
GTGCTTCAAAATGGGTAACC+TGG 0.628259 tig0025513:+27259 None:intergenic
GACTCTGCATAACGTATCAG+AGG 0.628578 tig0025513:-24652 Msa1423140:exon
CATAACGTATCAGAGGATCA+TGG 0.631520 tig0025513:-24645 Msa1423140:exon
GATGAAACATATGGAGATGG+GGG 0.631905 tig0025513:+25398 None:intergenic
AGAGATGGACAAAAGATGCA+AGG 0.632488 tig0025513:-25865 Msa1423140:CDS
TGCTTCAGAAAGTGAGCCTG+CGG 0.635679 tig0025513:-25600 Msa1423140:CDS
CAACTTGACGAAGTTATGGA+AGG 0.645660 tig0025513:-25692 Msa1423140:CDS
GAAGCTAGTGATGAAATGAA+GGG 0.646836 tig0025513:-26214 Msa1423140:CDS
CCGATAACTTGACAAGTCTG+TGG 0.655057 tig0025513:+25759 None:intergenic
ATTAATAGGAGTAAGGTACA+TGG 0.662022 tig0025513:-27429 Msa1423140:CDS
TGCGATAGTTGAGAAAGGCA+AGG 0.663759 tig0025513:+25010 None:intergenic
AGAGCTTCTGAATCTGTGGA+GGG 0.666121 tig0025513:-25734 Msa1423140:CDS
GAAATTTACCTGCTCCAGAG+AGG 0.668278 tig0025513:-27391 Msa1423140:CDS
GGTAATGCATCAGCAAATGG+AGG 0.677979 tig0025513:-27564 Msa1423140:CDS
GTTATGCAGAGTCAGAACTT+CGG 0.680514 tig0025513:+24662 None:intergenic
TTGGCACCTATCCAGTGTAG+CGG 0.682153 tig0025513:+25505 None:intergenic
ACAAGTATGCCAGATTGATG+GGG 0.683870 tig0025513:-27473 Msa1423140:CDS
AGGGTTATCAGTTTGCTGTG+AGG 0.684882 tig0025513:-25715 Msa1423140:CDS
ATGAAACATATGGAGATGGG+GGG 0.693657 tig0025513:+25399 None:intergenic
GCTCCAGAGAGGGATATCGT+AGG 0.703657 tig0025513:-27380 Msa1423140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATTTATTTGATTGATATA+TGG - tig0025513:26783-26802 Msa1423140:intron 15.0%
!! GAAATAAAAAATAAAGAGAA+CGG - tig0025513:26933-26952 Msa1423140:intron 15.0%
!! TTGGTTATTTACAAAAAAAA+AGG + tig0025513:27612-27631 None:intergenic 15.0%
!! TAATATGAATGATGAATATT+TGG + tig0025513:27631-27650 None:intergenic 15.0%
!! TTAGAAAAGATTGGATTAAT+AGG - tig0025513:24682-24701 Msa1423140:CDS 20.0%
!! AAGATAATTGTCATAATTCA+TGG + tig0025513:25075-25094 None:intergenic 20.0%
!!! AATTTCTTAACCATTTTGAA+AGG - tig0025513:25858-25877 Msa1423140:CDS 20.0%
!!! ATTTTCTCATTTGTTTTACA+GGG - tig0025513:26965-26984 Msa1423140:intron 20.0%
!! TATAAAAAAAACCTAATGAC+TGG + tig0025513:27154-27173 None:intergenic 20.0%
!!! ATATCATGAATATTTTACTC+AGG - tig0025513:27199-27218 Msa1423140:CDS 20.0%
!!! ATTATTTTTGTTGTACAGTT+GGG - tig0025513:27531-27550 Msa1423140:CDS 20.0%
!! AAGTGTATTTGATCTAAATT+TGG - tig0025513:27672-27691 Msa1423140:CDS 20.0%
!! ATTTTACTGTGAAGAAACAT+CGG - tig0025513:24781-24800 Msa1423140:intron 25.0%
! ATTATGTTGTGCTTCAAAAT+GGG + tig0025513:24877-24896 None:intergenic 25.0%
! ACAACATGTTAGAATTGATT+GGG + tig0025513:24914-24933 None:intergenic 25.0%
! AACAACATGTTAGAATTGAT+TGG + tig0025513:24915-24934 None:intergenic 25.0%
!! GTTGACTCATATCATAATTT+GGG - tig0025513:24978-24997 Msa1423140:intron 25.0%
! AGTATGACGAATACAAAATT+AGG - tig0025513:25023-25042 Msa1423140:CDS 25.0%
!! ACAAGATAACAAATCTTTTC+TGG - tig0025513:25213-25232 Msa1423140:intron 25.0%
!! CAAGATAACAAATCTTTTCT+GGG - tig0025513:25214-25233 Msa1423140:intron 25.0%
!!! AGATTTTTTGTAGATGTCAA+AGG - tig0025513:25767-25786 Msa1423140:CDS 25.0%
! TAAGAAATTGAACTACATCA+AGG + tig0025513:25847-25866 None:intergenic 25.0%
! TGAAATATTTCAAATGCTCT+TGG + tig0025513:25998-26017 None:intergenic 25.0%
! GAGTATAAAGTGAATACATT+TGG - tig0025513:26082-26101 Msa1423140:CDS 25.0%
! TGTATTATCTGAAGAATTGA+AGG + tig0025513:26131-26150 None:intergenic 25.0%
! AATCTTGATATTGAAAAGTG+AGG - tig0025513:26465-26484 Msa1423140:intron 25.0%
! ATCTACATGAATACTATTTG+AGG + tig0025513:26506-26525 None:intergenic 25.0%
!! ATAATCAAACTGATTCAGAT+AGG - tig0025513:26656-26675 Msa1423140:intron 25.0%
!!! GATTTTCTCATTTGTTTTAC+AGG - tig0025513:26964-26983 Msa1423140:intron 25.0%
!! TGTTAATTTTTCCAGTCATT+AGG - tig0025513:27140-27159 Msa1423140:intron 25.0%
! ACAGGTAAACATTTACTTAA+TGG - tig0025513:27277-27296 Msa1423140:CDS 25.0%
! CAACTGTACAACAAAAATAA+TGG + tig0025513:27532-27551 None:intergenic 25.0%
!!! CATTATTTTTGTTGTACAGT+TGG - tig0025513:27530-27549 Msa1423140:CDS 25.0%
CATGTAGAAAATGTTGATGT+TGG - tig0025513:24447-24466 Msa1423140:three_prime_UTR 30.0%
GGTTCAATGTTAGAAAAGAT+TGG - tig0025513:24673-24692 Msa1423140:CDS 30.0%
AGATTGGATTAATAGGAGTA+AGG - tig0025513:24689-24708 Msa1423140:CDS 30.0%
ATTAATAGGAGTAAGGTACA+TGG - tig0025513:24696-24715 Msa1423140:CDS 30.0%
GATTATGTTGTGCTTCAAAA+TGG + tig0025513:24878-24897 None:intergenic 30.0%
! GGTTGACTCATATCATAATT+TGG - tig0025513:24977-24996 Msa1423140:intron 30.0%
CTTAAAGCTGAACTAGATTT+GGG + tig0025513:25005-25024 None:intergenic 30.0%
ACTTAAAGCTGAACTAGATT+TGG + tig0025513:25006-25025 None:intergenic 30.0%
ACAATTATCTTCATTCTGTG+AGG - tig0025513:25084-25103 Msa1423140:CDS 30.0%
CAATTATCTTCATTCTGTGA+GGG - tig0025513:25085-25104 Msa1423140:CDS 30.0%
!!! GTTTGATGGGTTATTTTCAA+AGG - tig0025513:25138-25157 Msa1423140:CDS 30.0%
TGTATGGCATAAAAAAATGC+TGG + tig0025513:25179-25198 None:intergenic 30.0%
ACACCTAAAAACTGTACAAA+TGG + tig0025513:25326-25345 None:intergenic 30.0%
AGACCATCGAATCATTTAAT+TGG - tig0025513:25396-25415 Msa1423140:CDS 30.0%
ATTCGTCATCTTTAACCAAA+TGG + tig0025513:25583-25602 None:intergenic 30.0%
AATCCTTTGCAAATGATCTA+AGG - tig0025513:25600-25619 Msa1423140:CDS 30.0%
!! TAACCATTTTGAAAGGATAG+TGG - tig0025513:25865-25884 Msa1423140:CDS 30.0%
! TATCAAGAACTTTTAGTGCA+TGG + tig0025513:26201-26220 None:intergenic 30.0%
AATTCAAGATAACCCTAAGA+TGG - tig0025513:26315-26334 Msa1423140:CDS 30.0%
GTTTGATTATAATTCACCGT+TGG + tig0025513:26646-26665 None:intergenic 30.0%
TTTGGTTGAGATGAAACATA+TGG + tig0025513:26739-26758 None:intergenic 30.0%
GCATATTTGATATAGAGATC+AGG - tig0025513:26833-26852 Msa1423140:intron 30.0%
! GTTTTATGCTCGTTAACTTT+TGG - tig0025513:26888-26907 Msa1423140:intron 30.0%
!!! TTTGTTTTACAGGGCTTATT+TGG - tig0025513:26974-26993 Msa1423140:intron 30.0%
! TGTTGGTTAGAATAGAAGTT+TGG + tig0025513:27088-27107 None:intergenic 30.0%
!! TGGTATGTGTTTTGGATTAT+AGG + tig0025513:27227-27246 None:intergenic 30.0%
!! CTTTGTGTATATTTGCTGTA+GGG - tig0025513:27377-27396 Msa1423140:CDS 30.0%
AAAATACAGGATTCTGAATG+AGG + tig0025513:27425-27444 None:intergenic 30.0%
AGATCAAATACACTTAAGAC+CGG + tig0025513:27667-27686 None:intergenic 30.0%
GATGGTGATCATAACAACTT+AGG - tig0025513:24417-24436 Msa1423140:three_prime_UTR 35.0%
GAAAATGTTGATGTTGGACT+TGG - tig0025513:24453-24472 Msa1423140:three_prime_UTR 35.0%
ACTTGGAACATCCAATAGTT+TGG - tig0025513:24470-24489 Msa1423140:three_prime_UTR 35.0%
CACATTCAAGTCCAAACTAT+TGG + tig0025513:24484-24503 None:intergenic 35.0%
CAATAGTTTGGACTTGAATG+TGG - tig0025513:24482-24501 Msa1423140:three_prime_UTR 35.0%
TAACAAGTATGCCAGATTGA+TGG - tig0025513:24650-24669 Msa1423140:exon 35.0%
AACAAGTATGCCAGATTGAT+GGG - tig0025513:24651-24670 Msa1423140:exon 35.0%
ATCATTACTCGTCAACAAGA+TGG - tig0025513:24837-24856 Msa1423140:intron 35.0%
TCAACAAGATGGAAAATACC+AGG - tig0025513:24848-24867 Msa1423140:intron 35.0%
! TGGTAGATTACGACCATTTT+GGG - tig0025513:25252-25271 Msa1423140:intron 35.0%
AACAAACAACATCCCCAAAA+TGG + tig0025513:25268-25287 None:intergenic 35.0%
!! TTTTGGGGATGTTGTTTGTT+TGG - tig0025513:25268-25287 Msa1423140:intron 35.0%
CAGCCAATTAAATGATTCGA+TGG + tig0025513:25402-25421 None:intergenic 35.0%
AGCATCTTGTTCTGTGATAA+TGG + tig0025513:25468-25487 None:intergenic 35.0%
AATCATCGAAGCAATCAATG+TGG - tig0025513:25490-25509 Msa1423140:CDS 35.0%
TCTTAAACATCTCAGCCATT+TGG - tig0025513:25565-25584 Msa1423140:CDS 35.0%
ACTCCTTAGATCATTTGCAA+AGG + tig0025513:25606-25625 None:intergenic 35.0%
AGGAAGAATTTACTCATGCT+TGG - tig0025513:25648-25667 Msa1423140:CDS 35.0%
GGAAGAATTTACTCATGCTT+GGG - tig0025513:25649-25668 Msa1423140:CDS 35.0%
GAAGAATTTACTCATGCTTG+GGG - tig0025513:25650-25669 Msa1423140:CDS 35.0%
AAGAATTTACTCATGCTTGG+GGG - tig0025513:25651-25670 Msa1423140:CDS 35.0%
ATAAACTCCAGCGAAATGAA+TGG - tig0025513:25690-25709 Msa1423140:CDS 35.0%
TGAGATGGATGTATAGAGAA+TGG - tig0025513:25714-25733 Msa1423140:CDS 35.0%
GAGATGGATGTATAGAGAAT+GGG - tig0025513:25715-25734 Msa1423140:CDS 35.0%
TGTATAGAGAATGGGAGAAA+TGG - tig0025513:25723-25742 Msa1423140:CDS 35.0%
GTATAGAGAATGGGAGAAAT+GGG - tig0025513:25724-25743 Msa1423140:CDS 35.0%
GATGTCAAAGGCTTTCATTT+AGG - tig0025513:25779-25798 Msa1423140:CDS 35.0%
AATTGAACTACATCAAGGTC+AGG + tig0025513:25842-25861 None:intergenic 35.0%
AGAAGCTAGTGATGAAATGA+AGG - tig0025513:25910-25929 Msa1423140:CDS 35.0%
GAAGCTAGTGATGAAATGAA+GGG - tig0025513:25911-25930 Msa1423140:CDS 35.0%
! AATATCCATCTCTTTTGTGC+TGG + tig0025513:26057-26076 None:intergenic 35.0%
TGTATGAAGTTTGAGCATGT+AGG - tig0025513:26163-26182 Msa1423140:CDS 35.0%
CTCTTCAAGATATATCGAGA+TGG + tig0025513:26244-26263 None:intergenic 35.0%
CTCGATATATCTTGAAGAGA+TGG - tig0025513:26245-26264 Msa1423140:CDS 35.0%
GATTATAATTCACCGTTGGT+TGG + tig0025513:26642-26661 None:intergenic 35.0%
CAGACAACAGTATTTGAAGA+AGG + tig0025513:26697-26716 None:intergenic 35.0%
AGATGAAACATATGGAGATG+GGG + tig0025513:26731-26750 None:intergenic 35.0%
GAGATGAAACATATGGAGAT+GGG + tig0025513:26732-26751 None:intergenic 35.0%
TGAGATGAAACATATGGAGA+TGG + tig0025513:26733-26752 None:intergenic 35.0%
GATAAAAGCTTACCTGCAAA+GGG + tig0025513:26765-26784 None:intergenic 35.0%
TGATAAAAGCTTACCTGCAA+AGG + tig0025513:26766-26785 None:intergenic 35.0%
!!! TGGTTTTGAAACCAATGAAG+TGG - tig0025513:26994-27013 Msa1423140:intron 35.0%
TGATGATCTAACACAAGATC+AGG + tig0025513:27040-27059 None:intergenic 35.0%
TGGTTAGAATAGAAGTTTGG+CGG + tig0025513:27085-27104 None:intergenic 35.0%
TGTTGCATCTGATTATTGCT+TGG + tig0025513:27260-27279 None:intergenic 35.0%
AAGCAATAATCAGATGCAAC+AGG - tig0025513:27259-27278 Msa1423140:CDS 35.0%
!! GCTTTGTGTATATTTGCTGT+AGG - tig0025513:27376-27395 Msa1423140:CDS 35.0%
!! TGTATATTTGCTGTAGGGTA+TGG - tig0025513:27382-27401 Msa1423140:CDS 35.0%
! AGGATTCTGAATGAGGATTT+TGG + tig0025513:27418-27437 None:intergenic 35.0%
AATAATGGCATTGCCAATAC+AGG + tig0025513:27517-27536 None:intergenic 35.0%
ATATTAAAAGATACCCCGTC+CGG - tig0025513:27645-27664 Msa1423140:CDS 35.0%
AATGGCTCTCAAACTCAATC+TGG - tig0025513:24540-24559 Msa1423140:three_prime_UTR 40.0%
! TCTGGTAATGCATCAGCAAA+TGG - tig0025513:24558-24577 Msa1423140:three_prime_UTR 40.0%
ACAAGTATGCCAGATTGATG+GGG - tig0025513:24652-24671 Msa1423140:exon 40.0%
AACATTGAACCCCATCAATC+TGG + tig0025513:24664-24683 None:intergenic 40.0%
TAGGAGTAAGGTACATGGTA+TGG - tig0025513:24701-24720 Msa1423140:CDS 40.0%
TACATGGTATGGTGGTTTCT+AGG - tig0025513:24712-24731 Msa1423140:CDS 40.0%
AAATTTACCTGCTCCAGAGA+GGG - tig0025513:24735-24754 Msa1423140:intron 40.0%
TACTGTGAAGAAACATCGGA+AGG - tig0025513:24785-24804 Msa1423140:intron 40.0%
!! ATTTTGTGTTGCTCAAGCTG+TGG - tig0025513:24953-24972 Msa1423140:intron 40.0%
TTCTGTGAGGGAAAGAGATA+TGG - tig0025513:25097-25116 Msa1423140:CDS 40.0%
TCTGTGAGGGAAAGAGATAT+GGG - tig0025513:25098-25117 Msa1423140:CDS 40.0%
AGGGAAAGAGATATGGGTAA+AGG - tig0025513:25104-25123 Msa1423140:CDS 40.0%
TTTCTGGGCTGATGACAATA+TGG - tig0025513:25229-25248 Msa1423140:intron 40.0%
! GTGGTAGATTACGACCATTT+TGG - tig0025513:25251-25270 Msa1423140:intron 40.0%
! GGTAGATTACGACCATTTTG+GGG - tig0025513:25253-25272 Msa1423140:intron 40.0%
! CGGCCATTTGTACAGTTTTT+AGG - tig0025513:25320-25339 Msa1423140:intron 40.0%
! TGGCTGTTTCGAACTTTCAT+TGG - tig0025513:25416-25435 Msa1423140:CDS 40.0%
AATGTCTGGGAAAAAGCCTA+AGG - tig0025513:25442-25461 Msa1423140:CDS 40.0%
!! TGTTCTGTGATAATGGCCTT+AGG + tig0025513:25461-25480 None:intergenic 40.0%
!! TGGTTTTACCTGAAACAGAC+CGG - tig0025513:25510-25529 Msa1423140:CDS 40.0%
TCTGCCAAACACATGTATAC+CGG + tig0025513:25532-25551 None:intergenic 40.0%
GAGTTGTATATATGACCCCA+AGG - tig0025513:25622-25641 Msa1423140:CDS 40.0%
TAAATTCTTCCTCGTCCTTG+GGG + tig0025513:25640-25659 None:intergenic 40.0%
GTAAATTCTTCCTCGTCCTT+GGG + tig0025513:25641-25660 None:intergenic 40.0%
AGTAAATTCTTCCTCGTCCT+TGG + tig0025513:25642-25661 None:intergenic 40.0%
AGAATTTACTCATGCTTGGG+GGG - tig0025513:25652-25671 Msa1423140:CDS 40.0%
ATCTCAACCATTCATTTCGC+TGG + tig0025513:25700-25719 None:intergenic 40.0%
AGCGAAATGAATGGTTGAGA+TGG - tig0025513:25699-25718 Msa1423140:CDS 40.0%
CCTCCACTATCCTTTCAAAA+TGG + tig0025513:25871-25890 None:intergenic 40.0%
!! CCATTTTGAAAGGATAGTGG+AGG - tig0025513:25868-25887 Msa1423140:CDS 40.0%
GGATGTCTTCCAAAGCTAAT+GGG - tig0025513:25932-25951 Msa1423140:CDS 40.0%
! TCCAAAGCTAATGGGTAATG+TGG - tig0025513:25940-25959 Msa1423140:CDS 40.0%
! ACCACATTACCCATTAGCTT+TGG + tig0025513:25944-25963 None:intergenic 40.0%
AGAGATGGACAAAAGATGCA+AGG - tig0025513:26260-26279 Msa1423140:CDS 40.0%
ATGGACAAAAGATGCAAGGT+TGG - tig0025513:26264-26283 Msa1423140:CDS 40.0%
TGGACAAAAGATGCAAGGTT+GGG - tig0025513:26265-26284 Msa1423140:CDS 40.0%
ATGCAAGGTTGGGTAATTTC+AGG - tig0025513:26275-26294 Msa1423140:CDS 40.0%
TGCAAGGTTGGGTAATTTCA+GGG - tig0025513:26276-26295 Msa1423140:CDS 40.0%
!! AGCTTGCTACAACCATCTTA+GGG + tig0025513:26330-26349 None:intergenic 40.0%
CCACAGACTTGTCAAGTTAT+CGG - tig0025513:26366-26385 Msa1423140:CDS 40.0%
CAACTTGACGAAGTTATGGA+AGG - tig0025513:26433-26452 Msa1423140:intron 40.0%
GAGTAAGCGAAGAAATTGAC+AGG - tig0025513:26596-26615 Msa1423140:intron 40.0%
ATGAAACATATGGAGATGGG+GGG + tig0025513:26729-26748 None:intergenic 40.0%
GATGAAACATATGGAGATGG+GGG + tig0025513:26730-26749 None:intergenic 40.0%
CATGCATTGAACCACTTCAT+TGG + tig0025513:27008-27027 None:intergenic 40.0%
TAGAATAGAAGTTTGGCGGT+TGG + tig0025513:27081-27100 None:intergenic 40.0%
CATCTGATTATTGCTTGGCA+CGG + tig0025513:27255-27274 None:intergenic 40.0%
CGATAACCCACCAAAAGTTT+GGG + tig0025513:27326-27345 None:intergenic 40.0%
!!! CCAAACTTTTGGTGGGTTAT+CGG - tig0025513:27324-27343 Msa1423140:CDS 40.0%
!!! CAAACTTTTGGTGGGTTATC+GGG - tig0025513:27325-27344 Msa1423140:CDS 40.0%
ATGGTCACACAGCAAAATAC+AGG + tig0025513:27438-27457 None:intergenic 40.0%
AGTCAGAACTTCGGTATCTA+TGG + tig0025513:27457-27476 None:intergenic 40.0%
GTTATGCAGAGTCAGAACTT+CGG + tig0025513:27466-27485 None:intergenic 40.0%
CATAACGTATCAGAGGATCA+TGG - tig0025513:27480-27499 Msa1423140:CDS 40.0%
AAATACACTTAAGACCGGAC+GGG + tig0025513:27662-27681 None:intergenic 40.0%
CAAATACACTTAAGACCGGA+CGG + tig0025513:27663-27682 None:intergenic 40.0%
! CGTTCGGACTGCAACAATTT+TGG + tig0025513:24509-24528 None:intergenic 45.0%
CCATTAGCGTGAAGAACGTT+CGG + tig0025513:24525-24544 None:intergenic 45.0%
GGTAATGCATCAGCAAATGG+AGG - tig0025513:24561-24580 Msa1423140:three_prime_UTR 45.0%
! GGCTTTGTCAACACAGTGTT+CGG - tig0025513:24582-24601 Msa1423140:three_prime_UTR 45.0%
GTCAACACAGTGTTCGGAAT+CGG - tig0025513:24588-24607 Msa1423140:three_prime_UTR 45.0%
GAGTAAGGTACATGGTATGG+TGG - tig0025513:24704-24723 Msa1423140:CDS 45.0%
GAAATTTACCTGCTCCAGAG+AGG - tig0025513:24734-24753 Msa1423140:intron 45.0%
TTTCCTACGATATCCCTCTC+TGG + tig0025513:24751-24770 None:intergenic 45.0%
GTGCTTCAAAATGGGTAACC+TGG + tig0025513:24869-24888 None:intergenic 45.0%
GATATGGGTAAAGGAGAAGC+TGG - tig0025513:25113-25132 Msa1423140:CDS 45.0%
GTCATCAACATCGAGCTGTA+TGG + tig0025513:25195-25214 None:intergenic 45.0%
! GCAGAACAGACAGAGCTTTT+CGG - tig0025513:25300-25319 Msa1423140:intron 45.0%
ACTTTCATTGGCGCAATGTC+TGG - tig0025513:25428-25447 Msa1423140:CDS 45.0%
CTTTCATTGGCGCAATGTCT+GGG - tig0025513:25429-25448 Msa1423140:CDS 45.0%
CATGTATACCGGTCTGTTTC+AGG + tig0025513:25521-25540 None:intergenic 45.0%
CAGACCGGTATACATGTGTT+TGG - tig0025513:25525-25544 Msa1423140:CDS 45.0%
TATATATGACCCCAAGGACG+AGG - tig0025513:25628-25647 Msa1423140:CDS 45.0%
GGGATGTCTTCCAAAGCTAA+TGG - tig0025513:25931-25950 Msa1423140:CDS 45.0%
GTCAACCAGCACAAAAGAGA+TGG - tig0025513:26049-26068 Msa1423140:CDS 45.0%
!! CAGCTTGCTACAACCATCTT+AGG + tig0025513:26331-26350 None:intergenic 45.0%
CCGATAACTTGACAAGTCTG+TGG + tig0025513:26369-26388 None:intergenic 45.0%
AAGAGCTTCTGAATCTGTGG+AGG - tig0025513:26390-26409 Msa1423140:CDS 45.0%
AGAGCTTCTGAATCTGTGGA+GGG - tig0025513:26391-26410 Msa1423140:CDS 45.0%
!! AGGGTTATCAGTTTGCTGTG+AGG - tig0025513:26410-26429 Msa1423140:CDS 45.0%
GAGGCAACTTGACGAAGTTA+TGG - tig0025513:26429-26448 Msa1423140:intron 45.0%
TCAACCAAATCTCCCTTTGC+AGG - tig0025513:26750-26769 Msa1423140:intron 45.0%
CTTACCTGCAAAGGGAGATT+TGG + tig0025513:26757-26776 None:intergenic 45.0%
AAAGGCAAGGAGAACTCTGT+TGG + tig0025513:27105-27124 None:intergenic 45.0%
TGCGATAGTTGAGAAAGGCA+AGG + tig0025513:27118-27137 None:intergenic 45.0%
TTGCCTTTCTCAACTATCGC+AGG - tig0025513:27117-27136 Msa1423140:intron 45.0%
ACACCTGCGATAGTTGAGAA+AGG + tig0025513:27123-27142 None:intergenic 45.0%
! CGGACTCATGGTATGTGTTT+TGG + tig0025513:27235-27254 None:intergenic 45.0%
! AAACCCCCTTCCCAAACTTT+TGG - tig0025513:27313-27332 Msa1423140:CDS 45.0%
ACCCACCAAAAGTTTGGGAA+GGG + tig0025513:27321-27340 None:intergenic 45.0%
AACCCACCAAAAGTTTGGGA+AGG + tig0025513:27322-27341 None:intergenic 45.0%
CCGATAACCCACCAAAAGTT+TGG + tig0025513:27327-27346 None:intergenic 45.0%
GACTCTGCATAACGTATCAG+AGG - tig0025513:27473-27492 Msa1423140:CDS 45.0%
GGTCGAACCATATCCTGTAT+TGG - tig0025513:27501-27520 Msa1423140:CDS 45.0%
GGCATTGCCAATACAGGATA+TGG + tig0025513:27511-27530 None:intergenic 45.0%
AATACACTTAAGACCGGACG+GGG + tig0025513:27661-27680 None:intergenic 45.0%
CCGAACGTTCTTCACGCTAA+TGG - tig0025513:24522-24541 Msa1423140:three_prime_UTR 50.0%
CACAGTGTTCGGAATCGGTA+CGG - tig0025513:24593-24612 Msa1423140:three_prime_UTR 50.0%
GGAGACGAGAACTGGATGTT+TGG - tig0025513:24806-24825 Msa1423140:intron 50.0%
! TTGTGTTGCTCAAGCTGTGG+AGG - tig0025513:24956-24975 Msa1423140:intron 50.0%
AGGAGAAGCTGGACGTTTGA+TGG - tig0025513:25124-25143 Msa1423140:CDS 50.0%
GGAGAAGCTGGACGTTTGAT+GGG - tig0025513:25125-25144 Msa1423140:CDS 50.0%
CTGGGCTGATGACAATATGG+TGG - tig0025513:25232-25251 Msa1423140:intron 50.0%
!! GAGAAATGGGCTGTCTGCTT+TGG - tig0025513:25737-25756 Msa1423140:CDS 50.0%
GGCAAGAGCTTCTGAATCTG+TGG - tig0025513:26387-26406 Msa1423140:CDS 50.0%
TGCTTCAGAAAGTGAGCCTG+CGG - tig0025513:26525-26544 Msa1423140:intron 50.0%
TCCTCAGTCGCATGTCCATT+CGG + tig0025513:26559-26578 None:intergenic 50.0%
AATTGACAGGACCGCTACAC+TGG - tig0025513:26609-26628 Msa1423140:intron 50.0%
TTGGCACCTATCCAGTGTAG+CGG + tig0025513:26623-26642 None:intergenic 50.0%
ACTGGATAGGTGCCAACCAA+CGG - tig0025513:26627-26646 Msa1423140:intron 50.0%
AGAAGTTTGGCGGTTGGAAC+AGG + tig0025513:27075-27094 None:intergenic 50.0%
TATTGCTTGGCACGGACTCA+TGG + tig0025513:27247-27266 None:intergenic 50.0%
CCACCAAAAGTTTGGGAAGG+GGG + tig0025513:27319-27338 None:intergenic 50.0%
CCCACCAAAAGTTTGGGAAG+GGG + tig0025513:27320-27339 None:intergenic 50.0%
! CCCCTTCCCAAACTTTTGGT+GGG - tig0025513:27317-27336 Msa1423140:CDS 50.0%
!! GGTGGGTTATCGGGTGTTTA+TGG - tig0025513:27334-27353 Msa1423140:CDS 50.0%
GGGTTATCGGGTGTTTATGG+TGG - tig0025513:27337-27356 Msa1423140:CDS 50.0%
!! ACGATATCCCTCTCTGGAGC+AGG + tig0025513:24745-24764 None:intergenic 55.0%
GCTCCAGAGAGGGATATCGT+AGG - tig0025513:24745-24764 Msa1423140:intron 55.0%
CATCGGAAGGAGACGAGAAC+TGG - tig0025513:24798-24817 Msa1423140:intron 55.0%
TCATGCTTGGGGGGTTATGC+TGG - tig0025513:25661-25680 Msa1423140:CDS 55.0%
CCATTCGGAAAGATCACCGC+AGG + tig0025513:26544-26563 None:intergenic 55.0%
CCTGCGGTGATCTTTCCGAA+TGG - tig0025513:26541-26560 Msa1423140:intron 55.0%
TCCGAATGGACATGCGACTG+AGG - tig0025513:26555-26574 Msa1423140:intron 55.0%
TGGACATGCGACTGAGGATC+AGG - tig0025513:26561-26580 Msa1423140:intron 55.0%
ACAGGACCGCTACACTGGAT+AGG - tig0025513:26614-26633 Msa1423140:intron 55.0%
! CCCCCTTCCCAAACTTTTGG+TGG - tig0025513:27316-27335 Msa1423140:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0025513 gene 24414 27733 24414 ID=Msa1423140;Name=Msa1423140
tig0025513 mRNA 24414 27733 24414 ID=Msa1423140-mRNA-1;Parent=Msa1423140;Name=Msa1423140-mRNA-1;_AED=0.15;_eAED=0.25;_QI=0|0.75|0.6|1|1|1|5|249|644
tig0025513 exon 24414 24749 24414 ID=Msa1423140-mRNA-1:exon:3083;Parent=Msa1423140-mRNA-1
tig0025513 exon 24867 24926 24867 ID=Msa1423140-mRNA-1:exon:3082;Parent=Msa1423140-mRNA-1
tig0025513 exon 25009 25161 25009 ID=Msa1423140-mRNA-1:exon:3081;Parent=Msa1423140-mRNA-1
tig0025513 exon 25376 26446 25376 ID=Msa1423140-mRNA-1:exon:3080;Parent=Msa1423140-mRNA-1
tig0025513 exon 27170 27733 27170 ID=Msa1423140-mRNA-1:exon:3079;Parent=Msa1423140-mRNA-1
tig0025513 CDS 27170 27733 27170 ID=Msa1423140-mRNA-1:cds;Parent=Msa1423140-mRNA-1
tig0025513 CDS 25376 26446 25376 ID=Msa1423140-mRNA-1:cds;Parent=Msa1423140-mRNA-1
tig0025513 CDS 25009 25161 25009 ID=Msa1423140-mRNA-1:cds;Parent=Msa1423140-mRNA-1
tig0025513 CDS 24867 24926 24867 ID=Msa1423140-mRNA-1:cds;Parent=Msa1423140-mRNA-1
tig0025513 CDS 24663 24749 24663 ID=Msa1423140-mRNA-1:cds;Parent=Msa1423140-mRNA-1
tig0025513 three_prime_UTR 24414 24662 24414 ID=Msa1423140-mRNA-1:three_prime_utr;Parent=Msa1423140-mRNA-1
Gene Sequence

>Msa1423140

ATGGATGGTGATCATAACAACTTAGGTAATGTGCATGTAGAAAATGTTGATGTTGGACTTGGAACATCCAATAGTTTGGACTTGAATGTGGACCAAAATTGTTGCAGTCCGAACGTTCTTCACGCTAATGGCTCTCAAACTCAATCTGGTAATGCATCAGCAAATGGAGGCTTTGTCAACACAGTGTTCGGAATCGGTACGGTATTTGAGTCTGATGAACATGCATATAGATTCTATAACAAGTATGCCAGATTGATGGGGTTCAATGTTAGAAAAGATTGGATTAATAGGAGTAAGGTACATGGTATGGTGGTTTCTAGGAAATTTACCTGCTCCAGAGAGGGATATCGTAGGAAAGACGAGAGAGATTTTACTGTGAAGAAACATCGGAAGGAGACGAGAACTGGATGTTTGGCACACATGATCATTACTCGTCAACAAGATGGAAAATACCAGGTTACCCATTTTGAAGCACAACATAATCATGATGATATAAACCCAATCAATTCTAACATGTTGTTACTAGAGTTGCAAAATGAATTTTGTGTTGCTCAAGCTGTGGAGCGAAATGAATGGTTGAGATGGATGTATAGAGAATGGGAGAAATGGGCTGTCTGCTTTGGTCAGAATAGATTTTTTGTAGATGTCAAAGGCTTTCATTTAGGTGAGATGTTGTCTCACAAGCTTAGAAGCTATCTAAATCCTGACCTTGATGTAGTTCAATTTCTTAACCATTTTGAAAGGATAGTGGAGGAGCAACGATATAAAGAAATAGAAGCTAGTGATGAAATGAAGGGATGTCTTCCAAAGCTAATGGGTAATGTGGTTGTGTTGAAACATGCTAGTGTTGCTTATACTCCAAGAGCATTTGAAATATTTCAGCAACGATATGAGAACTCTTTGAATGTTATTGTCAACCAGCACAAAAGAGATGGATATTTGTTTGAGTATAAAGTGAATACATTTGGACATGCTAGACAGTACACTGTCACCTTCAATTCTTCAGATAATACAGTTGTCTGCAGTTGTATGAAGTTTGAGCATGTAGGTTTTCTTTGTAGCCATGCACTAAAAGTTCTTGATAATAGAAACATAAAAGTAGTTCCATCTCGATATATCTTGAAGAGATGGACAAAAGATGCAAGGTTGGGTAATTTCAGGGAGATTAAGCAATTTAAAATTCAAGATAACCCTAAGATGGTTGTAGCAAGCTGTTACAAAGATTTATGCCACAGACTTGTCAAGTTATCGGCAAGAGCTTCTGAATCTGTGGAGGGTTATCAGTTTGCTGTGAGGCAACTTGACGAAGTTATGGAAGGAGTGCAGAAAATCTTGATATTGAAAAGTGAGGAAGCTCAAGTTATCACCTCAAATAGTATTCATGTAGATGCTTCAGAAAGTGAGCCTGCGGTGATCTTTCCGAATGGACATGCGACTGAGGATCAGGATGAAAGTAACAGAGTAAGCGAAGAAATTGACAGGACCGCTACACTGGATAGGTGCCAACCAACGGTGAATTATAATCAAACTGATTCAGATAGGATTTTAAATGTAGAACCTTCTTCAAATACTGTTGTCTGCATTTCATGCCCCCCATCTCCATATGTTTCATCTCAACCAAATCTCCCTTTGCAGGGCTTATTTGGTTTTGAAACCAATGAAGTGGTTCAATGCATGTATGAACAGCCTGATCTTGTGTTAGATCATCAATCTAATACTAACCTGTTCCAACCGCCAAACTTCTATTCTAACCAACAGAGTTCTCCTTGCCTTTCTCAACTATCGCAGGAGCCTATAATCCAAAACACATACCATGAGTCCGTGCCAAGCAATAATCAGATGCAACAGGGTATGGATCTTGATATCCAAAATCCTCATTCAGAATCCTGTATTTTGCTGTGTGACCATAGATACCGAAGTTCTGACTCTGCATAA
Protein sequence

>Msa1423140

MDGDHNNLGNVHVENVDVGLGTSNSLDLNVDQNCCSPNVLHANGSQTQSGNASANGGFVNTVFGIGTVFESDEHAYRFYNKYARLMGFNVRKDWINRSKVHGMVVSRKFTCSREGYRRKDERDFTVKKHRKETRTGCLAHMIITRQQDGKYQVTHFEAQHNHDDINPINSNMLLLELQNEFCVAQAVERNEWLRWMYREWEKWAVCFGQNRFFVDVKGFHLGEMLSHKLRSYLNPDLDVVQFLNHFERIVEEQRYKEIEASDEMKGCLPKLMGNVVVLKHASVAYTPRAFEIFQQRYENSLNVIVNQHKRDGYLFEYKVNTFGHARQYTVTFNSSDNTVVCSCMKFEHVGFLCSHALKVLDNRNIKVVPSRYILKRWTKDARLGNFREIKQFKIQDNPKMVVASCYKDLCHRLVKLSARASESVEGYQFAVRQLDEVMEGVQKILILKSEEAQVITSNSIHVDASESEPAVIFPNGHATEDQDESNRVSEEIDRTATLDRCQPTVNYNQTDSDRILNVEPSSNTVVCISCPPSPYVSSQPNLPLQGLFGFETNEVVQCMYEQPDLVLDHQSNTNLFQPPNFYSNQQSSPCLSQLSQEPIIQNTYHESVPSNNQMQQGMDLDIQNPHSESCILLCDHRYRSSDSA*