AlfalfaGEDB | Msa1422830

M. sativa cultivar zhongmu-4 / Msa1422830

KEGG: -
GO: -
PFAM: PF03909.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1422830	A0A072VCG3	89.858	424	22	7	1	409	1	418	0.0	684

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0053810	Msa1422830	0.801706	7.933587e-49	-8.615850e-47
Msa0381360	Msa1422830	0.889483	2.327494e-73	-8.615850e-47
Msa0422530	Msa1422830	0.906508	1.376401e-80	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1422830	MtrunA17_Chr3g0135001	89.858	424	22	7	1	409	1	418	0.0	684
Msa1422830	MtrunA17_Chr5g0400951	36.150	426	243	9	3	406	2	420	1.65e-56	192
Msa1422830	MtrunA17_Chr4g0064381	38.182	110	65	2	144	253	129	235	4.09e-21	94.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1422830	AT1G10720.1	41.995	381	157	11	55	394	51	408	1.72e-66	218
Msa1422830	AT3G49800.1	36.215	428	227	10	3	394	2	419	1.24e-61	205
Msa1422830	AT5G65910.1	34.928	418	210	11	3	394	2	383	2.82e-50	175
Msa1422830	AT2G10950.1	34.975	203	109	7	62	253	25	215	5.83e-25	104
Msa1422830	AT2G10950.2	34.975	203	109	7	62	253	25	215	5.83e-25	104
Msa1422830	AT2G10950.3	34.975	203	109	7	62	253	25	215	5.83e-25	104

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Find 115 sgRNAs with CRISPR-Local

Find 312 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATCAAACATGGATAC+TGG	0.409853	tig0025489:+51438	Msa1422830:three_prime_UTR
AAACCCTAACTCGTCAATTT+TGG	0.118472	tig0025489:+47683	Msa1422830:CDS
AAAGAGAATTGCCAGATGTT+TGG	0.381680	tig0025489:+51413	Msa1422830:three_prime_UTR
AAATATGAGCATCTATTTCA+GGG	0.503499	tig0025489:+51712	Msa1422830:three_prime_UTR
AAATGCTGTGATGATATTTG+AGG	0.563683	tig0025489:+51510	Msa1422830:three_prime_UTR
AACAACTTCTTTGTCTGTGA+TGG	0.504969	tig0025489:+51011	Msa1422830:CDS
AACTTCAGATGACACGACTG+AGG	0.758277	tig0025489:+51347	Msa1422830:exon
AACTTTCACTCATATGACAA+GGG	0.678075	tig0025489:-48284	None:intergenic
AAGTACACAATTGAAAGTTC+TGG	0.380859	tig0025489:+51051	Msa1422830:CDS
AATAATGATGAAACGTTGCA+AGG	0.577943	tig0025489:+47625	Msa1422830:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAACAATAAACCATTCGA+AGG	+	tig0025489:47409-47428	Msa1422830:five_prime_UTR	30.0%
AATAATGATGAAACGTTGCA+AGG	+	tig0025489:47625-47644	Msa1422830:CDS	30.0%
TGAGATTGAGAAATAGTTGA+TGG	-	tig0025489:47742-47761	None:intergenic	30.0%
TTCATTCATAGCTACAGAAA+TGG	-	tig0025489:48149-48168	None:intergenic	30.0%
AACTTTCACTCATATGACAA+GGG	-	tig0025489:48287-48306	None:intergenic	30.0%
TAACTTTCACTCATATGACA+AGG	-	tig0025489:48288-48307	None:intergenic	30.0%
GCTTAATAAACAAGATTCTG+AGG	+	tig0025489:48344-48363	Msa1422830:CDS	30.0%
AAGGAATAAATGCAACAGTA+AGG	-	tig0025489:48715-48734	None:intergenic	30.0%
TTCCTTTAATTCATGTTGCT+TGG	+	tig0025489:48729-48748	Msa1422830:intron	30.0%
TAAGCCACAATATACAAAGA+GGG	-	tig0025489:48755-48774	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0025489	gene	47332	51945	47332	ID=Msa1422830;Name=Msa1422830
tig0025489	mRNA	47332	51945	47332	ID=Msa1422830-mRNA-1;Parent=Msa1422830;Name=Msa1422830-mRNA-1;_AED=0.00;_eAED=0.00;_QI=137\|1\|1\|1\|1\|1\|3\|598\|409
tig0025489	exon	47332	47994	47332	ID=Msa1422830-mRNA-1:exon:2860;Parent=Msa1422830-mRNA-1
tig0025489	exon	48196	48383	48196	ID=Msa1422830-mRNA-1:exon:2861;Parent=Msa1422830-mRNA-1
tig0025489	exon	50832	51945	50832	ID=Msa1422830-mRNA-1:exon:2862;Parent=Msa1422830-mRNA-1
tig0025489	five_prime_UTR	47332	47468	47332	ID=Msa1422830-mRNA-1:five_prime_utr;Parent=Msa1422830-mRNA-1
tig0025489	CDS	47469	47994	47469	ID=Msa1422830-mRNA-1:cds;Parent=Msa1422830-mRNA-1
tig0025489	CDS	48196	48383	48196	ID=Msa1422830-mRNA-1:cds;Parent=Msa1422830-mRNA-1
tig0025489	CDS	50832	51347	50832	ID=Msa1422830-mRNA-1:cds;Parent=Msa1422830-mRNA-1
tig0025489	three_prime_UTR	51348	51945	51348	ID=Msa1422830-mRNA-1:three_prime_utr;Parent=Msa1422830-mRNA-1

Gene Sequence

>Msa1422830

ATGTCATCATGGTTTGCTCGTTCCCTTGCAAACTCTCTCCGAATCGATGATGATGATGATGATGGTGACATTCAAAACGACGTCGTAACCGAATCTCCAACCACCTCACCTAGAAACAATAATAATAATGCAGATGAATCAGAAGAACAAGAAGAAAATAATGATGAAACGTTGCAAGGTCGTGGTGTTAAAGAAGACCTCGACGAAATCAAACAAACCCTAACTCGTCAATTTTGGGGTATGGCTTCTTTTCTCGCTCCTCCTCCTCCTCCATCAACTATTTCTCAATCTCAACAACAACAGCAAGTTGATGCCGATGTAATTTCAAATCAAAATTCTGAAATGGAACAAGGTGTGTTTCGTGGGGATGATCCTCAACCGAATTCGGAAGGAGAACAGGAAAGGGAATTTGATATACAATGCGCGGTTGGAATTACTGAGGAGGTTTTGACTTTTGCATTGAATATCGCTATGCATCCTGAAACCTGGTTGGATTTTCCAATTGACGAGGAAGATGATAACGACGATTTTGAAATGTCCGAGGACCAACGAGATCATGCTATGGTTGTTGAAAGGCTTGCTCCGAGATTAGCTGCGCTAAGAATTGAATTGTGCCCTTGTCATATGAGTGAAAGTTACTTTTGGAAAGTCTATTTTGTACTTTTGCATTCAAGGCTTAATAAACAAGATTCTGAGGTTTTGTCTACACCCCAGGTCATGGTAGCCAGATCAATGTGGATGCAGGAATTGCAGAAACAAACAAAGCCTGAATTTGAAATTTTCGGAAGAAGTGATCTCTACTCAAGAGACAATGCTCAGCATCATGATTCTACTCCCAGCTTATCAGATGACACCTATTCTGATGACATGCCTCATCGGACATTCGGATATAGAACAACTTCTTTGTCTGTGATGGCAGATAATGAGTCTGAAAAGTACACAATTGAAAGTTCTGGATCACATTTATCAGATAAGTCGGTTATTGAGGAAAATCCAAGTAATAAAACTGAAAACAAAGATCTAAAAAGTGGTCGAGCTTCTCAAATTATGATTCAGGACTATGATGACGACGGTGATGATGATTGGCCAGACGATGATTCTGATTTAGGGGGATATCGTGGAACAGCGCTCCCTATTGTGAATGAAGAAGATATATCTTTCAGCGATCTCGAGGATGATGATTATGGCATTAAACATGTTAGTAGCAATTCAGATTCAAAGGTGGTGTGA

Protein sequence

>Msa1422830

MSSWFARSLANSLRIDDDDDDGDIQNDVVTESPTTSPRNNNNNADESEEQEENNDETLQGRGVKEDLDEIKQTLTRQFWGMASFLAPPPPPSTISQSQQQQQVDADVISNQNSEMEQGVFRGDDPQPNSEGEQEREFDIQCAVGITEEVLTFALNIAMHPETWLDFPIDEEDDNDDFEMSEDQRDHAMVVERLAPRLAALRIELCPCHMSESYFWKVYFVLLHSRLNKQDSEVLSTPQVMVARSMWMQELQKQTKPEFEIFGRSDLYSRDNAQHHDSTPSLSDDTYSDDMPHRTFGYRTTSLSVMADNESEKYTIESSGSHLSDKSVIEENPSNKTENKDLKSGRASQIMIQDYDDDGDDDWPDDDSDLGGYRGTALPIVNEEDISFSDLEDDDYGIKHVSSNSDSKVV*