AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1424470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1424470 MtrunA17_Chr3g0109981 97.425 466 3 5 1 461 1 462 0.0 908
Msa1424470 MtrunA17_Chr8g0366281 48.837 215 71 6 257 451 157 352 1.90e-52 180
Msa1424470 MtrunA17_Chr6g0479431 35.666 443 194 16 18 451 7 367 6.78e-50 174
Msa1424470 MtrunA17_Chr5g0410741 50.000 222 67 8 255 453 201 401 5.02e-48 170
Msa1424470 MtrunA17_Chr1g0154261 47.179 195 74 5 267 461 120 285 1.44e-41 149
Msa1424470 MtrunA17_Chr7g0243081 64.179 67 23 1 266 332 65 130 2.61e-21 95.1
Msa1424470 MtrunA17_Chr1g0162931 65.517 58 20 0 272 329 17 74 1.72e-20 91.3
Msa1424470 MtrunA17_Chr1g0210911 63.793 58 21 0 272 329 21 78 5.42e-20 90.9
Msa1424470 MtrunA17_Chr2g0320231 68.966 58 18 0 272 329 96 153 1.86e-19 89.7
Msa1424470 MtrunA17_Chr1g0190301 43.860 114 48 3 253 352 143 254 2.23e-19 89.7
Msa1424470 MtrunA17_Chr8g0379121 67.241 58 19 0 272 329 110 167 6.14e-19 89.0
Msa1424470 MtrunA17_Chr7g0258551 48.837 86 41 2 275 359 214 297 2.99e-18 86.3
Msa1424470 MtrunA17_Chr7g0261911 50.000 86 43 0 274 359 263 348 4.30e-18 86.7
Msa1424470 MtrunA17_Chr2g0288601 66.038 53 18 0 272 324 70 122 8.05e-17 76.6
Msa1424470 MtrunA17_Chr3g0095611 58.929 56 23 0 274 329 20 75 1.12e-16 78.6
Msa1424470 MtrunA17_Chr1g0175091 57.377 61 26 0 275 335 260 320 3.10e-16 80.5
Msa1424470 MtrunA17_Chr5g0409731 67.925 53 17 0 274 326 26 78 3.11e-16 79.0
Msa1424470 MtrunA17_Chr7g0274471 64.286 56 20 0 274 329 219 274 3.23e-16 80.5
Msa1424470 MtrunA17_Chr2g0283951 58.462 65 23 1 272 332 88 152 8.90e-16 79.0
Msa1424470 MtrunA17_Chr7g0254981 63.636 55 20 0 275 329 52 106 1.27e-15 77.0
Msa1424470 MtrunA17_Chr1g0197171 54.930 71 31 1 274 344 45 114 1.37e-15 77.4
Msa1424470 MtrunA17_Chr2g0290991 54.286 70 31 1 274 343 40 108 1.49e-15 77.4
Msa1424470 MtrunA17_Chr8g0356501 51.429 70 34 0 274 343 38 107 2.14e-15 77.4
Msa1424470 MtrunA17_Chr2g0291021 63.636 55 20 0 275 329 51 105 3.99e-15 76.3
Msa1424470 MtrunA17_Chr2g0310011 51.429 70 34 0 274 343 36 105 4.00e-15 76.6
Msa1424470 MtrunA17_Chr2g0321461 58.929 56 23 0 274 329 46 101 7.89e-15 75.9
Msa1424470 MtrunA17_Chr6g0461641 54.237 59 27 0 274 332 43 101 3.84e-14 73.9
Msa1424470 MtrunA17_Chr5g0417521 52.632 57 27 0 274 330 16 72 4.91e-14 73.2
Msa1424470 MtrunA17_Chr4g0042451 54.386 57 26 0 274 330 18 74 1.38e-13 71.6
Msa1424470 MtrunA17_Chr1g0175081 49.180 61 31 0 275 335 24 84 2.30e-13 66.2
Msa1424470 MtrunA17_Chr1g0175071 37.615 109 52 3 275 367 179 287 8.41e-13 69.3
Msa1424470 MtrunA17_Chr7g0242521 55.172 58 23 1 275 329 15 72 5.11e-12 65.9
Msa1424470 MtrunA17_Chr4g0062921 48.529 68 35 0 272 339 234 301 1.26e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1424470 AT5G16560.1 40.122 491 173 17 1 458 1 403 1.07e-80 256
Msa1424470 AT1G32240.2 59.627 161 41 4 233 389 186 326 1.60e-49 173
Msa1424470 AT1G32240.1 57.225 173 48 5 223 389 174 326 4.08e-49 172
Msa1424470 AT4G17695.1 34.000 350 131 13 113 454 62 319 7.53e-40 146
Msa1424470 AT5G42630.2 72.727 88 24 0 268 355 100 187 2.99e-36 133
Msa1424470 AT5G42630.1 72.727 88 24 0 268 355 100 187 1.17e-35 133
Msa1424470 AT2G38300.1 33.838 198 100 6 253 447 38 207 1.24e-21 96.3
Msa1424470 AT2G40260.1 33.047 233 111 7 235 461 62 255 4.67e-20 92.4
Msa1424470 AT4G04605.1 67.241 58 19 0 272 329 13 70 4.92e-20 89.0
Msa1424470 AT2G02060.2 65.625 64 20 1 273 334 29 92 9.82e-20 89.0
Msa1424470 AT2G02060.1 65.625 64 20 1 273 334 29 92 9.82e-20 89.0
Msa1424470 AT1G14600.1 66.102 59 20 0 273 331 23 81 2.12e-19 88.2
Msa1424470 AT4G04580.1 59.322 59 24 0 274 332 15 73 3.29e-18 82.4
Msa1424470 AT2G42660.1 66.071 56 19 0 274 329 51 106 1.36e-17 82.8
Msa1424470 AT4G04555.1 58.621 58 24 0 272 329 13 70 1.82e-17 78.2
Msa1424470 AT5G45580.2 54.430 79 26 2 248 326 8 76 2.44e-17 82.4
Msa1424470 AT5G45580.1 67.925 53 17 0 274 326 8 60 4.53e-17 81.3
Msa1424470 AT4G28610.1 58.730 63 26 0 275 337 227 289 5.54e-16 80.1
Msa1424470 AT2G01060.1 63.636 55 20 0 275 329 17 71 2.84e-15 76.6
Msa1424470 AT3G12730.1 60.714 56 22 0 274 329 24 79 3.39e-15 75.5
Msa1424470 AT1G32240.3 54.651 86 28 3 308 389 1 79 4.40e-15 72.8
Msa1424470 AT3G04450.3 55.714 70 30 1 261 329 225 294 5.85e-15 75.9
Msa1424470 AT5G29000.4 55.385 65 29 0 275 339 233 297 7.23e-15 75.9
Msa1424470 AT4G13640.2 62.264 53 20 0 274 326 38 90 7.71e-15 75.5
Msa1424470 AT4G13640.1 62.264 53 20 0 274 326 38 90 7.87e-15 75.5
Msa1424470 AT1G79430.2 50.704 71 35 0 274 344 35 105 9.10e-15 75.9
Msa1424470 AT5G29000.1 63.636 55 20 0 275 329 190 244 1.03e-14 75.9
Msa1424470 AT5G29000.3 63.636 55 20 0 275 329 190 244 1.03e-14 75.9
Msa1424470 AT3G04450.1 55.714 70 30 1 261 329 225 294 1.20e-14 76.3
Msa1424470 AT3G04450.4 55.714 70 30 1 261 329 185 254 1.32e-14 75.9
Msa1424470 AT3G04450.2 55.714 70 30 1 261 329 185 254 1.32e-14 75.9
Msa1424470 AT3G24120.2 55.000 60 27 0 274 333 42 101 1.39e-14 74.7
Msa1424470 AT3G24120.1 55.000 60 27 0 274 333 42 101 1.41e-14 74.7
Msa1424470 AT5G29000.2 63.636 55 20 0 275 329 233 287 1.51e-14 75.9
Msa1424470 AT5G18240.2 52.308 65 31 0 274 338 46 110 1.80e-14 75.5
Msa1424470 AT5G18240.3 52.308 65 31 0 274 338 46 110 1.80e-14 75.5
Msa1424470 AT5G06800.3 56.364 55 24 0 275 329 194 248 1.86e-14 73.9
Msa1424470 AT5G18240.5 52.308 65 31 0 274 338 46 110 1.92e-14 75.5
Msa1424470 AT5G18240.1 52.308 65 31 0 274 338 46 110 1.94e-14 75.5
Msa1424470 AT5G18240.4 52.308 65 31 0 274 338 46 110 1.94e-14 75.5
Msa1424470 AT3G04030.1 49.231 65 33 0 274 338 46 110 3.88e-14 74.3
Msa1424470 AT5G06800.1 40.698 86 48 2 275 359 194 277 3.97e-14 74.3
Msa1424470 AT5G06800.2 37.079 89 52 1 275 359 194 282 4.40e-14 73.9
Msa1424470 AT3G04030.3 49.231 65 33 0 274 338 46 110 4.47e-14 74.3
Msa1424470 AT2G20400.1 57.812 64 27 0 275 338 233 296 5.40e-14 73.9
Msa1424470 AT2G20400.2 57.143 63 27 0 275 337 233 295 9.24e-14 73.2
Msa1424470 AT3G13040.3 61.818 55 21 0 275 329 243 297 2.52e-13 72.0
Msa1424470 AT3G13040.1 61.818 55 21 0 275 329 243 297 2.52e-13 72.0
Msa1424470 AT3G13040.2 61.818 55 21 0 275 329 243 297 2.52e-13 72.0
Msa1424470 AT2G06020.1 47.436 78 40 1 249 326 63 139 2.92e-13 70.9
Msa1424470 AT1G69580.1 50.877 57 28 0 274 330 35 91 3.13e-12 68.2
Msa1424470 AT2G03500.1 45.652 92 44 2 267 358 227 312 3.22e-12 68.6
Msa1424470 AT1G69580.2 50.877 57 28 0 274 330 35 91 3.42e-12 67.8
Msa1424470 AT3G04030.2 47.692 65 33 1 274 338 46 109 1.05e-11 67.0
Msa1424470 AT1G68670.1 43.678 87 47 1 272 358 214 298 2.16e-11 65.5
Msa1424470 AT5G58080.1 37.405 131 68 6 272 390 193 321 4.63e-11 65.5

Find 127 sgRNAs with CRISPR-Local

Find 331 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTCCATGGCTGGTAAAA+AGG 0.206571 tig0025713:-34484 None:intergenic
GTTCATGCTGTTGAGCTTCT+TGG 0.223702 tig0025713:+34876 Msa1424470:CDS
AGTAATAGAGACAACTAATA+TGG 0.235620 tig0025713:-39329 None:intergenic
GAGAGAGAGAGGAAGAAGAT+AGG 0.276433 tig0025713:-33463 None:intergenic
GTACTTGTCCATCGCATTCT+TGG 0.279145 tig0025713:-34837 None:intergenic
AGGGCATCTTCTTTAGAAAA+AGG 0.292586 tig0025713:-34002 None:intergenic
AGAATTATGAGGTTCATAAT+TGG 0.296903 tig0025713:-34139 None:intergenic
CACATGAATCACATAAATTA+TGG 0.301972 tig0025713:+34375 Msa1424470:CDS
AACTAACCTTTGATAATATA+TGG 0.305714 tig0025713:-33976 None:intergenic
TAGATATATAATATATAAAT+TGG 0.318867 tig0025713:+39411 Msa1424470:three_prime_UTR
AGTCCTTAATATTGTGTTTG+AGG 0.320622 tig0025713:+33819 Msa1424470:five_prime_UTR
CATCATCATCACTCACACTA+TGG 0.323012 tig0025713:+34729 Msa1424470:CDS
ATGGATGCGACCTATGAAAT+TGG 0.326048 tig0025713:+39765 Msa1424470:three_prime_UTR
AGAGAGAGAGGAAGAAGATA+GGG 0.328501 tig0025713:-33462 None:intergenic
ATAAGAACTAAGTAAGAATA+TGG 0.342340 tig0025713:-39704 None:intergenic
GAAGGAGACGGAGTTGAATT+AGG 0.345696 tig0025713:-34549 None:intergenic
GGTGTTGTTGATGTTGAAAT+TGG 0.348173 tig0025713:-34045 None:intergenic
GAGGATGATGTACCACAAAT+TGG 0.348731 tig0025713:+36191 Msa1424470:CDS
AGTCCTCAAACACAATATTA+AGG 0.351081 tig0025713:-33822 None:intergenic
CAAGCTTAATTAATTTCCTT+TGG 0.362356 tig0025713:+39303 Msa1424470:exon
ACTCATCCACCAACACTCTA+TGG 0.365181 tig0025713:+36288 Msa1424470:CDS
ATTTGATGTCCTCCTGATAT+TGG 0.366341 tig0025713:-38337 None:intergenic
GACAAACCTGCAGCTTCTTC+AGG 0.367603 tig0025713:+35798 Msa1424470:CDS
CCACCATTGCTTCCAATTTG+TGG 0.370237 tig0025713:-36203 None:intergenic
TGGTGATATAAACACATCTT+TGG 0.387535 tig0025713:-34522 None:intergenic
CCAAGCTTGGAGTTCACATT+AGG 0.389593 tig0025713:+39255 Msa1424470:CDS
TGTCCTTTGCCATTCCAATC+TGG 0.391114 tig0025713:-39282 None:intergenic
TAATCCATATCTTGCTGTAT+TGG 0.398561 tig0025713:-36266 None:intergenic
TTTGGACTTGTTTGTGGCCA+TGG 0.402795 tig0025713:-38274 None:intergenic
TTCCAATCACAACCAATATC+AGG 0.403005 tig0025713:+38325 Msa1424470:CDS
ATCAGGAGGACATCAAATTC+AGG 0.406345 tig0025713:+38342 Msa1424470:CDS
TCTCTTGAATGTTCCATGGC+TGG 0.423245 tig0025713:-34492 None:intergenic
CTCCTGATATTGGTTGTGAT+TGG 0.426736 tig0025713:-38327 None:intergenic
CAAAGTCAAGAGAAAGAAGA+AGG 0.430708 tig0025713:-33522 None:intergenic
AATTACTTCTGAAATTATGT+AGG 0.431842 tig0025713:-39455 None:intergenic
TAATTCTTCAAGAACAACTT+TGG 0.432310 tig0025713:+34155 Msa1424470:CDS
TAGACTGGAATCCCTTTGAT+TGG 0.435250 tig0025713:-34444 None:intergenic
TAGACCAATACAGCAAGATA+TGG 0.437170 tig0025713:+36262 Msa1424470:CDS
ATCAGTTCCAATACTGACTT+TGG 0.438924 tig0025713:-35239 None:intergenic
CTCTATGGAGTAATTCTTCA+AGG 0.442637 tig0025713:+36303 Msa1424470:CDS
CTGAAACCATCTATGTCGTT+TGG 0.446505 tig0025713:-38292 None:intergenic
TTAGGCCTTTCAGATGGATC+TGG 0.451188 tig0025713:+36167 Msa1424470:intron
TCACATTAGGCATACCAGAT+TGG 0.452124 tig0025713:+39268 Msa1424470:CDS
GTTATTAAACCTTGTGGCTA+CGG 0.456769 tig0025713:-34667 None:intergenic
ACAAGTCCAAACGACATAGA+TGG 0.458406 tig0025713:+38286 Msa1424470:CDS
ATGTCGTTTGGACTTGTTTG+TGG 0.463624 tig0025713:-38280 None:intergenic
CACTCACACTATGGGAGTGT+TGG 0.469128 tig0025713:+34738 Msa1424470:CDS
CAGCTCAAGAACAATTTGTC+AGG 0.473778 tig0025713:+39210 Msa1424470:CDS
ATTACTTCTGAAATTATGTA+GGG 0.474169 tig0025713:-39454 None:intergenic
TGAAGTGGAGAGAAGAATGA+TGG 0.475084 tig0025713:-33425 None:intergenic
GAGAGAGAGGAAGAAGATAG+GGG 0.480199 tig0025713:-33461 None:intergenic
GCTCACACTGAACTATCTCT+TGG 0.484804 tig0025713:+34189 Msa1424470:CDS
CCTAATGTGAACTCCAAGCT+TGG 0.485539 tig0025713:-39255 None:intergenic
GGTTGTGATTGGAAGATAGT+TGG 0.486453 tig0025713:-38316 None:intergenic
CATGCTGTTGAGCTTCTTGG+TGG 0.491060 tig0025713:+34879 Msa1424470:CDS
AGAGGTGAAGATGACGAAGA+AGG 0.493765 tig0025713:-34567 None:intergenic
TTTATTTATTGGTGAGGAGG+AGG 0.496472 tig0025713:+39526 Msa1424470:three_prime_UTR
TCTATAAGCCGCAGAGCCAC+TGG 0.501461 tig0025713:-34640 None:intergenic
GTTGTTCTTGAAGAATTATG+AGG 0.504302 tig0025713:-34150 None:intergenic
CAAACAAGAACCAATTTCAT+AGG 0.504444 tig0025713:-39775 None:intergenic
CTAATGTGAACTCCAAGCTT+GGG 0.507483 tig0025713:-39254 None:intergenic
GACGAAGTTATGTCATATTG+TGG 0.515199 tig0025713:-39374 None:intergenic
TTGTAACCATATATTATCAA+AGG 0.515599 tig0025713:+33970 Msa1424470:five_prime_UTR
AAAGTCAGTATTGGAACTGA+TGG 0.515750 tig0025713:+35241 Msa1424470:CDS
TTCAATGAAAATCCCTTCCA+AGG 0.520306 tig0025713:-34022 None:intergenic
AGCAACACCAAAGTCAGTAT+TGG 0.520531 tig0025713:+35232 Msa1424470:CDS
GTAATAGAGACAACTAATAT+GGG 0.520741 tig0025713:-39328 None:intergenic
AAGCATCCATGATGAGATCA+AGG 0.522822 tig0025713:+34775 Msa1424470:CDS
CTAAAGAAGATGCCCTTGGA+AGG 0.522865 tig0025713:+34009 Msa1424470:exon
GTGTGATACTCACGAATAGC+AGG 0.530831 tig0025713:-39737 None:intergenic
CACTATGGGAGTGTTGGACT+TGG 0.532107 tig0025713:+34744 Msa1424470:CDS
TGGAGAGAAGAATGATGGAA+TGG 0.532783 tig0025713:-33420 None:intergenic
GAGAAGAATGATGGAATGGA+AGG 0.535420 tig0025713:-33416 None:intergenic
AGTGAGGAAAATGAGCTTGT+TGG 0.536485 tig0025713:-34287 None:intergenic
ATTGAAATTGACAGAGCAAG+AGG 0.537499 tig0025713:-39651 None:intergenic
ATTACTCCATAGAGTGTTGG+TGG 0.540356 tig0025713:-36294 None:intergenic
CCACAAATTGGAAGCAATGG+TGG 0.541302 tig0025713:+36203 Msa1424470:CDS
GAAGAATTAGAAGTACTTGG+TGG 0.543073 tig0025713:-34093 None:intergenic
GGTTGTTGTTGATGTTGTTG+TGG 0.543246 tig0025713:-34243 None:intergenic
AATGAACGGTTATGATAGAC+TGG 0.547430 tig0025713:-34459 None:intergenic
ATCATCATCACTCACACTAT+GGG 0.548466 tig0025713:+34730 Msa1424470:CDS
AAGGGTTAAGACCAATCAAA+GGG 0.548805 tig0025713:+34433 Msa1424470:CDS
TAATATATAAATTGGTGTGA+AGG 0.552524 tig0025713:+39419 Msa1424470:three_prime_UTR
AGAGTGTAAGAACCCAAGCT+TGG 0.553018 tig0025713:+39242 Msa1424470:CDS
AAAGAAGAATTAGAAGTACT+TGG 0.553090 tig0025713:-34096 None:intergenic
GAATGATGGAATGGAAGGGA+AGG 0.554334 tig0025713:-33411 None:intergenic
TGATGATGATGGTGGTGCAA+TGG 0.555394 tig0025713:-34714 None:intergenic
CGTGAGTATCACACTTAACA+TGG 0.557883 tig0025713:+39746 Msa1424470:three_prime_UTR
TTATGAGGTTCATAATTGGA+TGG 0.559155 tig0025713:-34135 None:intergenic
GGAAAAGAAATATTCCAGAA+AGG 0.559752 tig0025713:-33359 None:intergenic
GAAGGGTTAAGACCAATCAA+AGG 0.563565 tig0025713:+34432 Msa1424470:CDS
TAAAGAAGATGCCCTTGGAA+GGG 0.566491 tig0025713:+34010 Msa1424470:exon
GTTGGATTAGGCCTTTCAGA+TGG 0.566808 tig0025713:+36161 Msa1424470:intron
GAATTACCTGAAGAAGCTGC+AGG 0.569581 tig0025713:-35804 None:intergenic
AAAGTCAAGAGAAAGAAGAA+GGG 0.571326 tig0025713:-33521 None:intergenic
AGGTTCGTTATTAAACCTTG+TGG 0.572358 tig0025713:-34673 None:intergenic
GTGTCATCAACATCATCAGA+AGG 0.572682 tig0025713:+34414 Msa1424470:CDS
GGATATTGATGAGATTTGAA+AGG 0.577391 tig0025713:-34693 None:intergenic
CTTATGTGAAGAGAAAGATC+AGG 0.587834 tig0025713:-34069 None:intergenic
AGAATTACTCCATAGAGTGT+TGG 0.593216 tig0025713:-36297 None:intergenic
ACAACTAATATGGGAACCAA+AGG 0.597571 tig0025713:-39319 None:intergenic
GAAGATGACGAAGAAGGAGA+CGG 0.598612 tig0025713:-34561 None:intergenic
CAGCATGAACAAATCTAGCA+TGG 0.601911 tig0025713:-34863 None:intergenic
ATTAAACCTTGTGGCTACGG+CGG 0.603335 tig0025713:-34664 None:intergenic
AAGTGCAATATTCACAAGCA+AGG 0.606675 tig0025713:-33875 None:intergenic
AGAAGAATGATGGAATGGAA+GGG 0.611147 tig0025713:-33415 None:intergenic
TATTCAAAAGCTATAGCACA+AGG 0.614588 tig0025713:+33679 Msa1424470:five_prime_UTR
GTACCACAAATTGGAAGCAA+TGG 0.620505 tig0025713:+36200 Msa1424470:CDS
ATACCAGATTGGAATGGCAA+AGG 0.624282 tig0025713:+39279 Msa1424470:CDS
TGAGAGCACCAAGAATGCGA+TGG 0.625062 tig0025713:+34829 Msa1424470:CDS
CCTCTCCAGATCCATCTGAA+AGG 0.630610 tig0025713:-36172 None:intergenic
GACATAACTTCGTCATGCAG+AGG 0.636843 tig0025713:+39383 Msa1424470:three_prime_UTR
TTGTTGTGATGTTGAAAGTG+AGG 0.638425 tig0025713:-34303 None:intergenic
TCAATGAAAATCCCTTCCAA+GGG 0.641425 tig0025713:-34021 None:intergenic
TGTCATCAACATCATCAGAA+GGG 0.646503 tig0025713:+34415 Msa1424470:CDS
ATTGGAACTGATGGATGTGA+AGG 0.646666 tig0025713:+35250 Msa1424470:CDS
TTTAAACTCCAGTGGCTCTG+CGG 0.648557 tig0025713:+34632 Msa1424470:CDS
CCTTTCAGATGGATCTGGAG+AGG 0.652978 tig0025713:+36172 Msa1424470:CDS
TTAGGCATACCAGATTGGAA+TGG 0.661276 tig0025713:+39273 Msa1424470:CDS
ATAGAGCCGCCGTAGCCACA+AGG 0.664238 tig0025713:+34658 Msa1424470:CDS
ATGTGAAGAGAAAGATCAGG+TGG 0.665926 tig0025713:-34066 None:intergenic
GTGAGTGATGATGATGATGG+TGG 0.675410 tig0025713:-34722 None:intergenic
GCTGCATATGTAGAAGAACA+AGG 0.676730 tig0025713:+39577 Msa1424470:three_prime_UTR
ACGGAGTTGAATTAGGCATG+TGG 0.690652 tig0025713:-34542 None:intergenic
CAATCACAACCAATATCAGG+AGG 0.694629 tig0025713:+38328 Msa1424470:CDS
AGTGTGAGTGATGATGATGA+TGG 0.709391 tig0025713:-34725 None:intergenic
TGATCAGTAGTAGAGAACCA+TGG 0.715843 tig0025713:+38257 Msa1424470:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTAATAAATTTATTTT+TGG + tig0025713:36121-36140 Msa1424470:intron 0.0%
!! ATTAAAATCAGATTAAAAAA+AGG - tig0025713:33906-33925 None:intergenic 10.0%
!!! TTTTAATCTGATTTTAATAA+AGG + tig0025713:33908-33927 Msa1424470:five_prime_UTR 10.0%
!!! TTTTCTTCTTTTAAATTTTT+TGG + tig0025713:34936-34955 Msa1424470:intron 10.0%
!! ATATATAAAAAATTTAAGCA+AGG - tig0025713:36061-36080 None:intergenic 10.0%
!!! TTTTTTATGAAGAATAAAAA+GGG + tig0025713:36504-36523 Msa1424470:intron 10.0%
!!! TTATATATGTTTTTTAAAGT+TGG + tig0025713:36906-36925 Msa1424470:intron 10.0%
!! GTAACATATAAATTTATAAA+TGG + tig0025713:37073-37092 Msa1424470:intron 10.0%
!!! AATATTATTTTTCCAATAAT+TGG - tig0025713:38021-38040 None:intergenic 10.0%
!!! TTTAATTTTAGATTTCATTT+TGG - tig0025713:38769-38788 None:intergenic 10.0%
!!! GAAATCTAAAATTAAATTAA+AGG + tig0025713:38773-38792 Msa1424470:intron 10.0%
!!! AATAATTGTTTTAAGAAGAA+AGG - tig0025713:33389-33408 None:intergenic 15.0%
!! TTAAAAGAAGAAAATTAAAG+TGG - tig0025713:34930-34949 None:intergenic 15.0%
!!! TCTTTTAAATTTTTTGGATA+CGG + tig0025713:34942-34961 Msa1424470:intron 15.0%
!! ATATCTTATTCAATACAAAT+AGG + tig0025713:35640-35659 Msa1424470:intron 15.0%
!! GTAATTATTAGACTAATTAA+GGG - tig0025713:35858-35877 None:intergenic 15.0%
!!! CTTAATTAGTCTAATAATTA+CGG + tig0025713:35857-35876 Msa1424470:intron 15.0%
!! AAAAAAATGTGAAAAGAATA+AGG - tig0025713:35943-35962 None:intergenic 15.0%
!! GAGTAATTATAAATTAATGT+TGG + tig0025713:36143-36162 Msa1424470:intron 15.0%
!! TTATAAATTAATGTTGGATT+AGG + tig0025713:36149-36168 Msa1424470:intron 15.0%
!!! CTTTTTTATGAAGAATAAAA+AGG + tig0025713:36503-36522 Msa1424470:intron 15.0%
!! TATAAATTGTTAATGTTACT+TGG - tig0025713:36891-36910 None:intergenic 15.0%
!! CTTCAAATTAATTTAAACTT+AGG - tig0025713:37370-37389 None:intergenic 15.0%
!! TATCAAAAACAAAATAAAGA+AGG - tig0025713:37437-37456 None:intergenic 15.0%
!! AAATATCTTGATTACTATAA+AGG - tig0025713:37585-37604 None:intergenic 15.0%
!!! AAGATATTTTAAAAATTGAG+AGG + tig0025713:37596-37615 Msa1424470:intron 15.0%
!! TGAAAAAAGTTTATAAATTC+TGG + tig0025713:38065-38084 Msa1424470:intron 15.0%
!! GAAACCTAAAATTAATTTAA+GGG - tig0025713:38458-38477 None:intergenic 15.0%
!! TGAAACCTAAAATTAATTTA+AGG - tig0025713:38459-38478 None:intergenic 15.0%
!! ATCTTAATATTTGTAACTTA+AGG + tig0025713:38657-38676 Msa1424470:intron 15.0%
!!! TAATTGAAAGCATTTTTTTT+TGG - tig0025713:38998-39017 None:intergenic 15.0%
!!! TTATTTTTTATTTATTGGTG+AGG + tig0025713:39520-39539 Msa1424470:three_prime_UTR 15.0%
!! AGAAAGCAAAAAAAAAAGAT+TGG - tig0025713:33642-33661 None:intergenic 20.0%
!! TTGTAACCATATATTATCAA+AGG + tig0025713:33970-33989 Msa1424470:five_prime_UTR 20.0%
!!! AACTAACCTTTGATAATATA+TGG - tig0025713:33979-33998 None:intergenic 20.0%
!! AATCTAATACAGTTGTAAAA+TGG - tig0025713:34980-34999 None:intergenic 20.0%
!!! ATTGTTGTTGTTATTATGTT+TGG + tig0025713:35159-35178 Msa1424470:intron 20.0%
!!! TATGTTTGGTGTTATATTAT+AGG + tig0025713:35173-35192 Msa1424470:intron 20.0%
!!! TAATTTAGATGGTGAATTTT+AGG + tig0025713:35209-35228 Msa1424470:intron 20.0%
!! ATATGAAATTGAAATGAAAC+TGG + tig0025713:35338-35357 Msa1424470:intron 20.0%
!! TATGAAATTGAAATGAAACT+GGG + tig0025713:35339-35358 Msa1424470:intron 20.0%
!! CGTAATTATTAGACTAATTA+AGG - tig0025713:35859-35878 None:intergenic 20.0%
!! TTCACATATATAGACTTTAA+AGG - tig0025713:35983-36002 None:intergenic 20.0%
!! AAATTTAAGCAAGGTAATTA+AGG - tig0025713:36052-36071 None:intergenic 20.0%
!! CAAAAGTAAATAAACAAAGT+CGG - tig0025713:36426-36445 None:intergenic 20.0%
!! TCCTAATATTACAAATTTCA+AGG + tig0025713:36759-36778 Msa1424470:intron 20.0%
!! ATATATGAAGGACTTAATTT+GGG - tig0025713:36853-36872 None:intergenic 20.0%
!! AATATATGAAGGACTTAATT+TGG - tig0025713:36854-36873 None:intergenic 20.0%
!! AATACATAGTCAATATATGA+AGG - tig0025713:36865-36884 None:intergenic 20.0%
!! CTATAATTGAAATCTGTTTA+TGG - tig0025713:36966-36985 None:intergenic 20.0%
!! AAACCTAACAAATTCTAATA+TGG + tig0025713:37126-37145 Msa1424470:intron 20.0%
!! AACATATGGTAATAATTTCA+TGG - tig0025713:37186-37205 None:intergenic 20.0%
!! TTATTACCATATGTTGTATT+TGG + tig0025713:37191-37210 Msa1424470:intron 20.0%
!! TAAGTGGAAATTTAAGTAAA+GGG - tig0025713:37226-37245 None:intergenic 20.0%
!! TTAAGTGGAAATTTAAGTAA+AGG - tig0025713:37227-37246 None:intergenic 20.0%
!! TATAATTCGTGTAATTTAAG+TGG - tig0025713:37242-37261 None:intergenic 20.0%
!! ATTATATTAGAATAACCATG+TGG + tig0025713:37256-37275 Msa1424470:intron 20.0%
!!! CTTTTGCTTTTATTTTTTGA+TGG - tig0025713:37306-37325 None:intergenic 20.0%
!!! TCAAAAAAAAAAAGGTGTAT+AGG - tig0025713:37396-37415 None:intergenic 20.0%
!!! TATACACCTTTTTTTTTTTG+AGG + tig0025713:37395-37414 Msa1424470:intron 20.0%
!!! ATACACCTTTTTTTTTTTGA+GGG + tig0025713:37396-37415 Msa1424470:intron 20.0%
!! ACAAAATAAAGAAGGTTTAT+AGG - tig0025713:37429-37448 None:intergenic 20.0%
!! AGATATTTCATGTTTACTTA+AGG - tig0025713:37758-37777 None:intergenic 20.0%
!! CAAGTATATGATATATTGAT+AGG - tig0025713:37918-37937 None:intergenic 20.0%
!! GGAAAAATAATATTCTACAT+TGG + tig0025713:38027-38046 Msa1424470:intron 20.0%
!!! TGATGCAATTTTTTTTTTCT+TGG + tig0025713:38207-38226 Msa1424470:intron 20.0%
!!! TAGTCCCTTAAATTAATTTT+AGG + tig0025713:38451-38470 Msa1424470:intron 20.0%
!! AATGAAACGAAAAAAACTTA+AGG - tig0025713:38549-38568 None:intergenic 20.0%
!!! AAGTTTTTTTCGTTTCATTT+TGG + tig0025713:38550-38569 Msa1424470:intron 20.0%
!!! GACATTTTAGTTTATCATTT+CGG + tig0025713:38624-38643 Msa1424470:intron 20.0%
!!! ACATTTTAGTTTATCATTTC+GGG + tig0025713:38625-38644 Msa1424470:intron 20.0%
!! TAATATATAAATTGGTGTGA+AGG + tig0025713:39419-39438 Msa1424470:three_prime_UTR 20.0%
!!! ATTACTTCTGAAATTATGTA+GGG - tig0025713:39457-39476 None:intergenic 20.0%
!!! AATTACTTCTGAAATTATGT+AGG - tig0025713:39458-39477 None:intergenic 20.0%
!! ATAAGAACTAAGTAAGAATA+TGG - tig0025713:39707-39726 None:intergenic 20.0%
!!! TGTTTTAAGAAGAAAGGAAA+AGG - tig0025713:33383-33402 None:intergenic 25.0%
!!! TCTTTTTTTAGGCTTTTCTT+TGG - tig0025713:33766-33785 None:intergenic 25.0%
!!! GCTCTTTGTTTTCTTTTTTT+AGG - tig0025713:33777-33796 None:intergenic 25.0%
! TTGAACAAAAACAAAAGGAT+TGG + tig0025713:33845-33864 Msa1424470:five_prime_UTR 25.0%
!!! AAAGAAGAATTAGAAGTACT+TGG - tig0025713:34099-34118 None:intergenic 25.0%
! AGAATTATGAGGTTCATAAT+TGG - tig0025713:34142-34161 None:intergenic 25.0%
! TAATTCTTCAAGAACAACTT+TGG + tig0025713:34155-34174 Msa1424470:CDS 25.0%
!!! TTTTGATAATAAGGGTTATG+TGG - tig0025713:34267-34286 None:intergenic 25.0%
!!! CTTGTTGGTTTTGATAATAA+GGG - tig0025713:34275-34294 None:intergenic 25.0%
! CACATGAATCACATAAATTA+TGG + tig0025713:34375-34394 Msa1424470:CDS 25.0%
! GAGAAAAATGTTTACTTTCA+TGG - tig0025713:34905-34924 None:intergenic 25.0%
!! GGTGTTATATTATAGGTATT+TGG + tig0025713:35180-35199 Msa1424470:intron 25.0%
! TTTGGCTAATGTAATTTAGA+TGG + tig0025713:35198-35217 Msa1424470:intron 25.0%
! TTAAGTAGTAAGCTAAAAGA+AGG + tig0025713:35435-35454 Msa1424470:intron 25.0%
! TAAAATATGCATTAATCACG+AGG - tig0025713:35608-35627 None:intergenic 25.0%
! AATTTCTTGTGTTGAAATTG+TGG + tig0025713:35693-35712 Msa1424470:intron 25.0%
! GACTAATTAAGGGAAAAAAA+TGG - tig0025713:35848-35867 None:intergenic 25.0%
!! TATATATGTGAACACTTTTG+AGG + tig0025713:35991-36010 Msa1424470:intron 25.0%
!!! TATTTACTTTTGCTTTTGCA+TGG + tig0025713:36434-36453 Msa1424470:intron 25.0%
! AAGGTACAAAAGATATACAA+AGG - tig0025713:36588-36607 None:intergenic 25.0%
! GCCTTGAAATTTGTAATATT+AGG - tig0025713:36763-36782 None:intergenic 25.0%
! TCAAGATCCATATCAATATT+CGG - tig0025713:36804-36823 None:intergenic 25.0%
! TATAGTGTTACACTTCATAT+TGG + tig0025713:36981-37000 Msa1424470:intron 25.0%
!! TGTCAACTTATCAACTATTT+TGG - tig0025713:37022-37041 None:intergenic 25.0%
!! GATAAGTTGACAATATAATC+TGG + tig0025713:37030-37049 Msa1424470:intron 25.0%
! ACATTTCCAAATACAACATA+TGG - tig0025713:37200-37219 None:intergenic 25.0%
!! AGATTGATTTAGATTCAAAG+AGG + tig0025713:37324-37343 Msa1424470:intron 25.0%
!! GATTGATTTAGATTCAAAGA+GGG + tig0025713:37325-37344 Msa1424470:intron 25.0%
! ACTTTCCCTCAAAAAAAAAA+AGG - tig0025713:37404-37423 None:intergenic 25.0%
! TCTCATTAAGTAAACATTAG+TGG + tig0025713:37809-37828 Msa1424470:intron 25.0%
! AAAAAATTGCATCAGTTCAA+TGG - tig0025713:38201-38220 None:intergenic 25.0%
! GTCTAACGTTTGTAATTTAA+GGG - tig0025713:38517-38536 None:intergenic 25.0%
! TGTCTAACGTTTGTAATTTA+AGG - tig0025713:38518-38537 None:intergenic 25.0%
!!! CATTTTAGTTTATCATTTCG+GGG + tig0025713:38626-38645 Msa1424470:intron 25.0%
! CCTGAATTGAAATTCATTAA+TGG - tig0025713:38863-38882 None:intergenic 25.0%
! CCATTAATGAATTTCAATTC+AGG + tig0025713:38860-38879 Msa1424470:intron 25.0%
!!! TTTTAGGAAAATGGTCAATA+TGG - tig0025713:38959-38978 None:intergenic 25.0%
!!! CTTTACGATTTTTAGGAAAA+TGG - tig0025713:38968-38987 None:intergenic 25.0%
!! CATTTTCCTAAAAATCGTAA+AGG + tig0025713:38966-38985 Msa1424470:intron 25.0%
!! ACTTAACCTTTACGATTTTT+AGG - tig0025713:38975-38994 None:intergenic 25.0%
! CAAGCTTAATTAATTTCCTT+TGG + tig0025713:39303-39322 Msa1424470:exon 25.0%
! GTAATAGAGACAACTAATAT+GGG - tig0025713:39331-39350 None:intergenic 25.0%
! AGTAATAGAGACAACTAATA+TGG - tig0025713:39332-39351 None:intergenic 25.0%
!!! TTTTTTATTTATTGGTGAGG+AGG + tig0025713:39523-39542 Msa1424470:three_prime_UTR 25.0%
GGAAAAGAAATATTCCAGAA+AGG - tig0025713:33362-33381 None:intergenic 30.0%
AAAGTCAAGAGAAAGAAGAA+GGG - tig0025713:33524-33543 None:intergenic 30.0%
AAGCTAAGAAGAAAAAGAAG+AGG - tig0025713:33576-33595 None:intergenic 30.0%
TATTCAAAAGCTATAGCACA+AGG + tig0025713:33679-33698 Msa1424470:five_prime_UTR 30.0%
AGTCCTTAATATTGTGTTTG+AGG + tig0025713:33819-33838 Msa1424470:five_prime_UTR 30.0%
AGTCCTCAAACACAATATTA+AGG - tig0025713:33825-33844 None:intergenic 30.0%
GGACTTTGAACAAAAACAAA+AGG + tig0025713:33840-33859 Msa1424470:five_prime_UTR 30.0%
TTATGAGGTTCATAATTGGA+TGG - tig0025713:34138-34157 None:intergenic 30.0%
GTTGTTCTTGAAGAATTATG+AGG - tig0025713:34153-34172 None:intergenic 30.0%
!!! CTTGGAAGAAATTTTAGTTG+TGG + tig0025713:34207-34226 Msa1424470:CDS 30.0%
!!! GCTTGTTGGTTTTGATAATA+AGG - tig0025713:34276-34295 None:intergenic 30.0%
! TTTTTCTCTTGAATGTTCCA+TGG - tig0025713:34499-34518 None:intergenic 30.0%
TGGTGATATAAACACATCTT+TGG - tig0025713:34525-34544 None:intergenic 30.0%
GGATATTGATGAGATTTGAA+AGG - tig0025713:34696-34715 None:intergenic 30.0%
TCATGTAAAAAGTCATTTGC+AGG + tig0025713:35286-35305 Msa1424470:CDS 30.0%
GATAATAAGGTTGTAATGAG+AGG - tig0025713:35576-35595 None:intergenic 30.0%
!! GAGGTTGATGTTTGATAATA+AGG - tig0025713:35589-35608 None:intergenic 30.0%
TTTAAGCAAGGTAATTAAGG+TGG - tig0025713:36049-36068 None:intergenic 30.0%
! GTCAATTTTCAGATCAAAGA+GGG + tig0025713:36231-36250 Msa1424470:CDS 30.0%
! TAATCCATATCTTGCTGTAT+TGG - tig0025713:36269-36288 None:intergenic 30.0%
!! CTTCATAAAAAAGAGTGTTG+TGG - tig0025713:36496-36515 None:intergenic 30.0%
AAGAATAAAAAGGGTTCTTG+TGG + tig0025713:36513-36532 Msa1424470:intron 30.0%
! TCTTTTGTACCTTAGTGATT+TGG + tig0025713:36595-36614 Msa1424470:intron 30.0%
TCATAACCCGAATATTGATA+TGG + tig0025713:36794-36813 Msa1424470:intron 30.0%
CAAGATCCATATCAATATTC+GGG - tig0025713:36803-36822 None:intergenic 30.0%
!! TTGGGTTTTATGTAATCTAC+AGG - tig0025713:36835-36854 None:intergenic 30.0%
TTGCACAAATCAAGAAATAG+TGG + tig0025713:36930-36949 Msa1424470:intron 30.0%
! GGTCCATATTAGAATTTGTT+AGG - tig0025713:37132-37151 None:intergenic 30.0%
! ACCTCTAAAAAATAGTCTAC+AGG + tig0025713:37149-37168 Msa1424470:intron 30.0%
!!! ACCTGTAGACTATTTTTTAG+AGG - tig0025713:37153-37172 None:intergenic 30.0%
ATTTAAACTTAGGTGCAAGA+GGG - tig0025713:37360-37379 None:intergenic 30.0%
AATTTAAACTTAGGTGCAAG+AGG - tig0025713:37361-37380 None:intergenic 30.0%
GATTACTATAAAGGTAGAAC+TGG - tig0025713:37576-37595 None:intergenic 30.0%
AATGACAGACAGTAGTTAAT+AGG - tig0025713:37685-37704 None:intergenic 30.0%
CATTAAGTAAACATTAGTGG+TGG + tig0025713:37812-37831 Msa1424470:intron 30.0%
AAGGCTCATAATAGTAAGTT+TGG + tig0025713:37851-37870 Msa1424470:intron 30.0%
GAAATATACTCGAGTGTAAT+TGG + tig0025713:37873-37892 Msa1424470:intron 30.0%
TAGTTGTAATACATAGCCTT+AGG + tig0025713:37956-37975 Msa1424470:intron 30.0%
TGTCCAACATTCATAACTTA+AGG + tig0025713:38688-38707 Msa1424470:intron 30.0%
GTCCAACATTCATAACTTAA+GGG + tig0025713:38689-38708 Msa1424470:intron 30.0%
! TACAAGCTAGTTTCTTAAGT+TGG - tig0025713:38726-38745 None:intergenic 30.0%
TTGTACAAGTTTGTAGCTTA+AGG + tig0025713:38741-38760 Msa1424470:intron 30.0%
TGTACAAGTTTGTAGCTTAA+GGG + tig0025713:38742-38761 Msa1424470:intron 30.0%
CTAATGATTGTTTCCATTAC+AGG + tig0025713:39173-39192 Msa1424470:intron 30.0%
! AGAAGAATGATGGAATGGAA+GGG - tig0025713:33418-33437 None:intergenic 35.0%
! CTCATCACACTTTTCTTTCA+TGG + tig0025713:33497-33516 Msa1424470:five_prime_UTR 35.0%
CAAAGTCAAGAGAAAGAAGA+AGG - tig0025713:33525-33544 None:intergenic 35.0%
AGAAGAAAAAGAAGAGGTCA+TGG - tig0025713:33570-33589 None:intergenic 35.0%
!! GCTTTTCTTTGGAAAGAATC+TGG - tig0025713:33755-33774 None:intergenic 35.0%
AAGTGCAATATTCACAAGCA+AGG - tig0025713:33878-33897 None:intergenic 35.0%
! GCATCTTCTTTAGAAAAAGG+TGG - tig0025713:34002-34021 None:intergenic 35.0%
! AGGGCATCTTCTTTAGAAAA+AGG - tig0025713:34005-34024 None:intergenic 35.0%
!! TTTTCTAAAGAAGATGCCCT+TGG + tig0025713:34005-34024 Msa1424470:exon 35.0%
TCAATGAAAATCCCTTCCAA+GGG - tig0025713:34024-34043 None:intergenic 35.0%
TTCAATGAAAATCCCTTCCA+AGG - tig0025713:34025-34044 None:intergenic 35.0%
!! GGTGTTGTTGATGTTGAAAT+TGG - tig0025713:34048-34067 None:intergenic 35.0%
CTTATGTGAAGAGAAAGATC+AGG - tig0025713:34072-34091 None:intergenic 35.0%
!! GAAGAATTAGAAGTACTTGG+TGG - tig0025713:34096-34115 None:intergenic 35.0%
TTGTTGTGATGTTGAAAGTG+AGG - tig0025713:34306-34325 None:intergenic 35.0%
TGTCATCAACATCATCAGAA+GGG + tig0025713:34415-34434 Msa1424470:CDS 35.0%
AAGGGTTAAGACCAATCAAA+GGG + tig0025713:34433-34452 Msa1424470:CDS 35.0%
AATGAACGGTTATGATAGAC+TGG - tig0025713:34462-34481 None:intergenic 35.0%
CTGGAGTTTAAAAACGACAT+GGG - tig0025713:34624-34643 None:intergenic 35.0%
ACTGGAGTTTAAAAACGACA+TGG - tig0025713:34625-34644 None:intergenic 35.0%
!!! ATGTCGTTTTTAAACTCCAG+TGG + tig0025713:34624-34643 Msa1424470:CDS 35.0%
GTTATTAAACCTTGTGGCTA+CGG - tig0025713:34670-34689 None:intergenic 35.0%
AGGTTCGTTATTAAACCTTG+TGG - tig0025713:34676-34695 None:intergenic 35.0%
ATCATCATCACTCACACTAT+GGG + tig0025713:34730-34749 Msa1424470:CDS 35.0%
CAAAAACCTTGATCTCATCA+TGG - tig0025713:34784-34803 None:intergenic 35.0%
! CTTCTTTTTGTCGGCAATTT+AGG - tig0025713:34807-34826 None:intergenic 35.0%
!! ATCAGTTCCAATACTGACTT+TGG - tig0025713:35242-35261 None:intergenic 35.0%
! AAAGTCAGTATTGGAACTGA+TGG + tig0025713:35241-35260 Msa1424470:CDS 35.0%
ATAGCCAATGAAGAAGAAAG+TGG + tig0025713:35366-35385 Msa1424470:intron 35.0%
CAGTGAATGAATGAATGTGT+GGG + tig0025713:35391-35410 Msa1424470:intron 35.0%
TGAGAGGATTGTTGCATTAT+TGG - tig0025713:35560-35579 None:intergenic 35.0%
! CGTCAATTTTCAGATCAAAG+AGG + tig0025713:36230-36249 Msa1424470:CDS 35.0%
TAGACCAATACAGCAAGATA+TGG + tig0025713:36262-36281 Msa1424470:CDS 35.0%
AGAATTACTCCATAGAGTGT+TGG - tig0025713:36300-36319 None:intergenic 35.0%
CTCTATGGAGTAATTCTTCA+AGG + tig0025713:36303-36322 Msa1424470:CDS 35.0%
! TTTGTACCTTAGTGATTTGG+TGG + tig0025713:36598-36617 Msa1424470:intron 35.0%
TAATCGAATGCTCATTCGTT+AGG + tig0025713:36659-36678 Msa1424470:intron 35.0%
TGCTCATTCGTTAGGATAAA+TGG + tig0025713:36667-36686 Msa1424470:intron 35.0%
CAAACATACACACAAGTCAA+AGG - tig0025713:36737-36756 None:intergenic 35.0%
AGGCATGTCTTTCTCTTATT+AGG + tig0025713:37616-37635 Msa1424470:intron 35.0%
TAAGTAAACATTAGTGGTGG+TGG + tig0025713:37815-37834 Msa1424470:intron 35.0%
TTACTATTATGAGCCTTGCA+TGG - tig0025713:37848-37867 None:intergenic 35.0%
! GTAATACATAGCCTTAGGAT+TGG + tig0025713:37961-37980 Msa1424470:intron 35.0%
TACCACTTAAACCAATCCTA+AGG - tig0025713:37975-37994 None:intergenic 35.0%
ATAATTGGAGATGCTCTTGA+TGG - tig0025713:38006-38025 None:intergenic 35.0%
!! TCAAGAGCATCTCCAATTAT+TGG + tig0025713:38006-38025 Msa1424470:intron 35.0%
!! TGCATGAACTGTTTTGTTGA+TGG - tig0025713:38157-38176 None:intergenic 35.0%
CAAAACAGTTCATGCATGTT+TGG + tig0025713:38161-38180 Msa1424470:intron 35.0%
TTCCAATCACAACCAATATC+AGG + tig0025713:38325-38344 Msa1424470:CDS 35.0%
ATTTGATGTCCTCCTGATAT+TGG - tig0025713:38340-38359 None:intergenic 35.0%
GTCCCTTAAGTTATGAATGT+TGG - tig0025713:38694-38713 None:intergenic 35.0%
TTTGAATCACACTCCTGTAA+TGG - tig0025713:39189-39208 None:intergenic 35.0%
ACAACTAATATGGGAACCAA+AGG - tig0025713:39322-39341 None:intergenic 35.0%
GACGAAGTTATGTCATATTG+TGG - tig0025713:39377-39396 None:intergenic 35.0%
TTTATTTATTGGTGAGGAGG+AGG + tig0025713:39526-39545 Msa1424470:three_prime_UTR 35.0%
ATTGAAATTGACAGAGCAAG+AGG - tig0025713:39654-39673 None:intergenic 35.0%
! GAGAAGAATGATGGAATGGA+AGG - tig0025713:33419-33438 None:intergenic 40.0%
! TGGAGAGAAGAATGATGGAA+TGG - tig0025713:33423-33442 None:intergenic 40.0%
TGAAGTGGAGAGAAGAATGA+TGG - tig0025713:33428-33447 None:intergenic 40.0%
!!! GGAAGACTAGTGTTTTGAAG+TGG - tig0025713:33443-33462 None:intergenic 40.0%
AGAGAGAGAGGAAGAAGATA+GGG - tig0025713:33465-33484 None:intergenic 40.0%
TAAAGAAGATGCCCTTGGAA+GGG + tig0025713:34010-34029 Msa1424470:exon 40.0%
ATGTGAAGAGAAAGATCAGG+TGG - tig0025713:34069-34088 None:intergenic 40.0%
! GGTTGTTGTTGATGTTGTTG+TGG - tig0025713:34246-34265 None:intergenic 40.0%
AGTGAGGAAAATGAGCTTGT+TGG - tig0025713:34290-34309 None:intergenic 40.0%
GTGTCATCAACATCATCAGA+AGG + tig0025713:34414-34433 Msa1424470:CDS 40.0%
GAAGGGTTAAGACCAATCAA+AGG + tig0025713:34432-34451 Msa1424470:CDS 40.0%
TAGACTGGAATCCCTTTGAT+TGG - tig0025713:34447-34466 None:intergenic 40.0%
CTGGTAAAAAGGGGAATGAA+CGG - tig0025713:34476-34495 None:intergenic 40.0%
TGTTCCATGGCTGGTAAAAA+GGG - tig0025713:34486-34505 None:intergenic 40.0%
ATGTTCCATGGCTGGTAAAA+AGG - tig0025713:34487-34506 None:intergenic 40.0%
! AGTGTGAGTGATGATGATGA+TGG - tig0025713:34728-34747 None:intergenic 40.0%
CATCATCATCACTCACACTA+TGG + tig0025713:34729-34748 Msa1424470:CDS 40.0%
AAGCATCCATGATGAGATCA+AGG + tig0025713:34775-34794 Msa1424470:CDS 40.0%
! GCTCTCATGCTTCTTTTTGT+CGG - tig0025713:34816-34835 None:intergenic 40.0%
CAGCATGAACAAATCTAGCA+TGG - tig0025713:34866-34885 None:intergenic 40.0%
CTTGTGAGAGTGAGACTATT+AGG + tig0025713:35102-35121 Msa1424470:intron 40.0%
TGAGAGTGAGACTATTAGGT+GGG + tig0025713:35106-35125 Msa1424470:intron 40.0%
AGCAACACCAAAGTCAGTAT+TGG + tig0025713:35232-35251 Msa1424470:CDS 40.0%
! ATTGGAACTGATGGATGTGA+AGG + tig0025713:35250-35269 Msa1424470:CDS 40.0%
TGCACCACTTTCTTCTTCAT+TGG - tig0025713:35373-35392 None:intergenic 40.0%
GCAGTGAATGAATGAATGTG+TGG + tig0025713:35390-35409 Msa1424470:intron 40.0%
AATGAATGAATGTGTGGGAG+TGG + tig0025713:35396-35415 Msa1424470:intron 40.0%
GAGGATGATGTACCACAAAT+TGG + tig0025713:36191-36210 Msa1424470:CDS 40.0%
GTACCACAAATTGGAAGCAA+TGG + tig0025713:36200-36219 Msa1424470:CDS 40.0%
!! ATTACTCCATAGAGTGTTGG+TGG - tig0025713:36297-36316 None:intergenic 40.0%
!! AAAAAGAGTGTTGTGGACGT+GGG - tig0025713:36489-36508 None:intergenic 40.0%
!! AAAAAAGAGTGTTGTGGACG+TGG - tig0025713:36490-36509 None:intergenic 40.0%
!! ACCTTAGTGATTTGGTGGAT+TGG + tig0025713:36603-36622 Msa1424470:intron 40.0%
ACCAATCCACCAAATCACTA+AGG - tig0025713:36607-36626 None:intergenic 40.0%
!! AGCCTTAGGATTGGTTTAAG+TGG + tig0025713:37970-37989 Msa1424470:intron 40.0%
TGATCAGTAGTAGAGAACCA+TGG + tig0025713:38257-38276 Msa1424470:intron 40.0%
! ATGTCGTTTGGACTTGTTTG+TGG - tig0025713:38283-38302 None:intergenic 40.0%
ACAAGTCCAAACGACATAGA+TGG + tig0025713:38286-38305 Msa1424470:CDS 40.0%
CTGAAACCATCTATGTCGTT+TGG - tig0025713:38295-38314 None:intergenic 40.0%
GGTTGTGATTGGAAGATAGT+TGG - tig0025713:38319-38338 None:intergenic 40.0%
! CTCCTGATATTGGTTGTGAT+TGG - tig0025713:38330-38349 None:intergenic 40.0%
CAATCACAACCAATATCAGG+AGG + tig0025713:38328-38347 Msa1424470:CDS 40.0%
ATCAGGAGGACATCAAATTC+AGG + tig0025713:38342-38361 Msa1424470:CDS 40.0%
CAGCTCAAGAACAATTTGTC+AGG + tig0025713:39210-39229 Msa1424470:CDS 40.0%
CTAATGTGAACTCCAAGCTT+GGG - tig0025713:39257-39276 None:intergenic 40.0%
TCACATTAGGCATACCAGAT+TGG + tig0025713:39268-39287 Msa1424470:CDS 40.0%
TTAGGCATACCAGATTGGAA+TGG + tig0025713:39273-39292 Msa1424470:CDS 40.0%
ATACCAGATTGGAATGGCAA+AGG + tig0025713:39279-39298 Msa1424470:CDS 40.0%
GCTGCATATGTAGAAGAACA+AGG + tig0025713:39577-39596 Msa1424470:three_prime_UTR 40.0%
CGTGAGTATCACACTTAACA+TGG + tig0025713:39746-39765 Msa1424470:three_prime_UTR 40.0%
ATGGATGCGACCTATGAAAT+TGG + tig0025713:39765-39784 Msa1424470:three_prime_UTR 40.0%
! GAATGATGGAATGGAAGGGA+AGG - tig0025713:33414-33433 None:intergenic 45.0%
GAGAGAGAGGAAGAAGATAG+GGG - tig0025713:33464-33483 None:intergenic 45.0%
GAGAGAGAGAGGAAGAAGAT+AGG - tig0025713:33466-33485 None:intergenic 45.0%
AGAGAGAAAGAGAGAGAGAG+AGG - tig0025713:33477-33496 None:intergenic 45.0%
CTAAAGAAGATGCCCTTGGA+AGG + tig0025713:34009-34028 Msa1424470:exon 45.0%
GCTCACACTGAACTATCTCT+TGG + tig0025713:34189-34208 Msa1424470:CDS 45.0%
! ATTCCCCTTTTTACCAGCCA+TGG + tig0025713:34479-34498 Msa1424470:CDS 45.0%
GTTCCATGGCTGGTAAAAAG+GGG - tig0025713:34485-34504 None:intergenic 45.0%
TCTCTTGAATGTTCCATGGC+TGG - tig0025713:34495-34514 None:intergenic 45.0%
ACGGAGTTGAATTAGGCATG+TGG - tig0025713:34545-34564 None:intergenic 45.0%
GAAGGAGACGGAGTTGAATT+AGG - tig0025713:34552-34571 None:intergenic 45.0%
GAAGATGACGAAGAAGGAGA+CGG - tig0025713:34564-34583 None:intergenic 45.0%
AGAGGTGAAGATGACGAAGA+AGG - tig0025713:34570-34589 None:intergenic 45.0%
! TTTAAACTCCAGTGGCTCTG+CGG + tig0025713:34632-34651 Msa1424470:CDS 45.0%
ATTAAACCTTGTGGCTACGG+CGG - tig0025713:34667-34686 None:intergenic 45.0%
!! TGATGATGATGGTGGTGCAA+TGG - tig0025713:34717-34736 None:intergenic 45.0%
!! GTGAGTGATGATGATGATGG+TGG - tig0025713:34725-34744 None:intergenic 45.0%
GTACTTGTCCATCGCATTCT+TGG - tig0025713:34840-34859 None:intergenic 45.0%
GTTCATGCTGTTGAGCTTCT+TGG + tig0025713:34876-34895 Msa1424470:CDS 45.0%
GTGAGAGTGAGACTATTAGG+TGG + tig0025713:35105-35124 Msa1424470:intron 45.0%
ATCAGAGACTGTGTCATGTC+TGG - tig0025713:35482-35501 None:intergenic 45.0%
GAATTACCTGAAGAAGCTGC+AGG - tig0025713:35807-35826 None:intergenic 45.0%
! GTTGGATTAGGCCTTTCAGA+TGG + tig0025713:36161-36180 Msa1424470:intron 45.0%
TTAGGCCTTTCAGATGGATC+TGG + tig0025713:36167-36186 Msa1424470:intron 45.0%
CCACCATTGCTTCCAATTTG+TGG - tig0025713:36206-36225 None:intergenic 45.0%
CCACAAATTGGAAGCAATGG+TGG + tig0025713:36203-36222 Msa1424470:CDS 45.0%
ACTCATCCACCAACACTCTA+TGG + tig0025713:36288-36307 Msa1424470:CDS 45.0%
ACCATGTGGTGAAATCCTTG+TGG + tig0025713:37270-37289 Msa1424470:intron 45.0%
ACCACAAGGATTTCACCACA+TGG - tig0025713:37274-37293 None:intergenic 45.0%
GATGGAAAATCACGACCACA+AGG - tig0025713:37288-37307 None:intergenic 45.0%
!! GTAAACATTAGTGGTGGTGG+AGG + tig0025713:37818-37837 Msa1424470:intron 45.0%
! TTTGGACTTGTTTGTGGCCA+TGG - tig0025713:38277-38296 None:intergenic 45.0%
AGAGTGTAAGAACCCAAGCT+TGG + tig0025713:39242-39261 Msa1424470:CDS 45.0%
CCTAATGTGAACTCCAAGCT+TGG - tig0025713:39258-39277 None:intergenic 45.0%
CCAAGCTTGGAGTTCACATT+AGG + tig0025713:39255-39274 Msa1424470:CDS 45.0%
TGTCCTTTGCCATTCCAATC+TGG - tig0025713:39285-39304 None:intergenic 45.0%
GACATAACTTCGTCATGCAG+AGG + tig0025713:39383-39402 Msa1424470:three_prime_UTR 45.0%
GTGTGATACTCACGAATAGC+AGG - tig0025713:39740-39759 None:intergenic 45.0%
!!! ATTAAATACAATTTTTTTTT+TGG - tig0025713:38411-38430 None:intergenic 5.0%
!!! AAAAAAATTGTATTTAATTT+AGG + tig0025713:38413-38432 Msa1424470:intron 5.0%
!! TAGATATATAATATATAAAT+TGG + tig0025713:39411-39430 Msa1424470:three_prime_UTR 5.0%
!!! TGTATTTATTTTTTATTTAT+TGG + tig0025713:39515-39534 Msa1424470:three_prime_UTR 5.0%
TTTCCCCCTACATAGCTGCA+GGG + tig0025713:34589-34608 Msa1424470:CDS 50.0%
AAACCCCCTGCAGCTATGTA+GGG - tig0025713:34597-34616 None:intergenic 50.0%
TAAACCCCCTGCAGCTATGT+AGG - tig0025713:34598-34617 None:intergenic 50.0%
! CACTCACACTATGGGAGTGT+TGG + tig0025713:34738-34757 Msa1424470:CDS 50.0%
!! CACTATGGGAGTGTTGGACT+TGG + tig0025713:34744-34763 Msa1424470:CDS 50.0%
!! TGAGAGCACCAAGAATGCGA+TGG + tig0025713:34829-34848 Msa1424470:CDS 50.0%
CATGCTGTTGAGCTTCTTGG+TGG + tig0025713:34879-34898 Msa1424470:CDS 50.0%
GACAAACCTGCAGCTTCTTC+AGG + tig0025713:35798-35817 Msa1424470:CDS 50.0%
CCTCTCCAGATCCATCTGAA+AGG - tig0025713:36175-36194 None:intergenic 50.0%
CCTTTCAGATGGATCTGGAG+AGG + tig0025713:36172-36191 Msa1424470:CDS 50.0%
GCAGCTATGTAGGGGGAAAG+AGG - tig0025713:34588-34607 None:intergenic 55.0%
CTTTCCCCCTACATAGCTGC+AGG + tig0025713:34588-34607 Msa1424470:CDS 55.0%
TTCCCCCTACATAGCTGCAG+GGG + tig0025713:34590-34609 Msa1424470:CDS 55.0%
AACCCCCTGCAGCTATGTAG+GGG - tig0025713:34596-34615 None:intergenic 55.0%
TCTATAAGCCGCAGAGCCAC+TGG - tig0025713:34643-34662 None:intergenic 55.0%
TGGTGGAGGCTTACCATGCA+AGG + tig0025713:37832-37851 Msa1424470:intron 55.0%
TCCCCCTACATAGCTGCAGG+GGG + tig0025713:34591-34610 Msa1424470:CDS 60.0%
ACCCCCTGCAGCTATGTAGG+GGG - tig0025713:34595-34614 None:intergenic 60.0%
ATAGAGCCGCCGTAGCCACA+AGG + tig0025713:34658-34677 Msa1424470:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0025713 gene 33350 39790 33350 ID=Msa1424470;Name=Msa1424470
tig0025713 mRNA 33350 39790 33350 ID=Msa1424470-mRNA-1;Parent=Msa1424470;Name=Msa1424470-mRNA-1;_AED=0.03;_eAED=0.03;_QI=648|0.83|0.85|1|0.83|0.71|7|479|461
tig0025713 exon 33350 33596 33350 ID=Msa1424470-mRNA-1:exon:3792;Parent=Msa1424470-mRNA-1
tig0025713 exon 33617 34909 33617 ID=Msa1424470-mRNA-1:exon:3793;Parent=Msa1424470-mRNA-1
tig0025713 exon 35231 35307 35231 ID=Msa1424470-mRNA-1:exon:3794;Parent=Msa1424470-mRNA-1
tig0025713 exon 35774 35819 35774 ID=Msa1424470-mRNA-1:exon:3795;Parent=Msa1424470-mRNA-1
tig0025713 exon 36171 36324 36171 ID=Msa1424470-mRNA-1:exon:3796;Parent=Msa1424470-mRNA-1
tig0025713 exon 38264 38363 38264 ID=Msa1424470-mRNA-1:exon:3797;Parent=Msa1424470-mRNA-1
tig0025713 exon 39195 39790 39195 ID=Msa1424470-mRNA-1:exon:3798;Parent=Msa1424470-mRNA-1
tig0025713 five_prime_UTR 33350 33596 33350 ID=Msa1424470-mRNA-1:five_prime_utr;Parent=Msa1424470-mRNA-1
tig0025713 five_prime_UTR 33617 34017 33617 ID=Msa1424470-mRNA-1:five_prime_utr;Parent=Msa1424470-mRNA-1
tig0025713 CDS 34018 34909 34018 ID=Msa1424470-mRNA-1:cds;Parent=Msa1424470-mRNA-1
tig0025713 CDS 35231 35307 35231 ID=Msa1424470-mRNA-1:cds;Parent=Msa1424470-mRNA-1
tig0025713 CDS 35774 35819 35774 ID=Msa1424470-mRNA-1:cds;Parent=Msa1424470-mRNA-1
tig0025713 CDS 36171 36324 36171 ID=Msa1424470-mRNA-1:cds;Parent=Msa1424470-mRNA-1
tig0025713 CDS 38264 38363 38264 ID=Msa1424470-mRNA-1:cds;Parent=Msa1424470-mRNA-1
tig0025713 CDS 39195 39311 39195 ID=Msa1424470-mRNA-1:cds;Parent=Msa1424470-mRNA-1
tig0025713 three_prime_UTR 39312 39790 39312 ID=Msa1424470-mRNA-1:three_prime_utr;Parent=Msa1424470-mRNA-1
Gene Sequence

>Msa1424470

ATGCCCTTGGAAGGGATTTTCATTGAACCAATTTCAACATCAACAACACCACCTGATCTTTCTCTTCACATAAGTCCACCAAGTACTTCTAATTCTTCTTTGATTAACACAACTCAACCATCCAATTATGAACCTCATAATTCTTCAAGAACAACTTTGGCTCAAGCTCAAGCTCACACTGAACTATCTCTTGGAAGAAATTTTAGTTGTGGTGCTCAAGAACAACCACAACAACATCAACAACAACCACATAACCCTTATTATCAAAACCAACAAGCTCATTTTCCTCACTTTCAACATCACAACAATCACAGCTCTACAACAACAACAACAAGTACTACAACAAATTCTTCAAGTCACATGAATCACATAAATTATGGTGTTTCATTGCTTGATGTGTCATCAACATCATCAGAAGGGTTAAGACCAATCAAAGGGATTCCAGTCTATCATAACCGTTCATTCCCCTTTTTACCAGCCATGGAACATTCAAGAGAAAAAGATCCAAAGATGTGTTTATATCACCACATGCCTAATTCAACTCCGTCTCCTTCTTCGTCATCTTCACCTCTTTCCCCCTACATAGCTGCAGGGGGTTTAGATCCCATGTCGTTTTTAAACTCCAGTGGCTCTGCGGCTTATAGAGCCGCCGTAGCCACAAGGTTTAATAACGAACCTTTCAAATCTCATCAATATCCATTGCACCACCATCATCATCATCACTCACACTATGGGAGTGTTGGACTTGGTAGCTCAGAAGCATCCATGATGAGATCAAGGTTTTTGCCTAAATTGCCGACAAAAAGAAGCATGAGAGCACCAAGAATGCGATGGACAAGTACACTCCATGCTAGATTTGTTCATGCTGTTGAGCTTCTTGGTGGCCATGAAAGAGCAACACCAAAGTCAGTATTGGAACTGATGGATGTGAAGGATCTCACATTAGCTCATGTAAAAAGTCATTTGCAGATGTATCGAACTGTTAAGACAACTGACAAACCTGCAGCTTCTTCAGGCCTTTCAGATGGATCTGGAGAGGATGATGTACCACAAATTGGAAGCAATGGTGGAATTCGTCAATTTTCAGATCAAAGAGGGCTAACAGATAGACCAATACAGCAAGATATGGATTACTCATCCACCAACACTCTATGGAGTAATTCTTCAAGTAGTAGAGAACCATGGCCACAAACAAGTCCAAACGACATAGATGGTTTCAGACCAACTATCTTCCAATCACAACCAATATCAGGAGGACATCAAATTCAGGAGTGTGATTCAAATCAGCTCAAGAACAATTTGTCAGGATCTAATTTAGAGTGTAAGAACCCAAGCTTGGAGTTCACATTAGGCATACCAGATTGGAATGGCAAAGGACAAGCTTAA
Protein sequence

>Msa1424470

MPLEGIFIEPISTSTTPPDLSLHISPPSTSNSSLINTTQPSNYEPHNSSRTTLAQAQAHTELSLGRNFSCGAQEQPQQHQQQPHNPYYQNQQAHFPHFQHHNNHSSTTTTTSTTTNSSSHMNHINYGVSLLDVSSTSSEGLRPIKGIPVYHNRSFPFLPAMEHSREKDPKMCLYHHMPNSTPSPSSSSSPLSPYIAAGGLDPMSFLNSSGSAAYRAAVATRFNNEPFKSHQYPLHHHHHHHSHYGSVGLGSSEASMMRSRFLPKLPTKRSMRAPRMRWTSTLHARFVHAVELLGGHERATPKSVLELMDVKDLTLAHVKSHLQMYRTVKTTDKPAASSGLSDGSGEDDVPQIGSNGGIRQFSDQRGLTDRPIQQDMDYSSTNTLWSNSSSSREPWPQTSPNDIDGFRPTIFQSQPISGGHQIQECDSNQLKNNLSGSNLECKNPSLEFTLGIPDWNGKGQA*