Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1426700 | XP_013464879.1 | 97.955 | 538 | 11 | 0 | 1 | 538 | 1 | 538 | 0.0 | 1099 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1426700 | sp|P36616|DSK1_SCHPO | 43.633 | 479 | 240 | 6 | 13 | 477 | 54 | 516 | 3.52e-134 | 403 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1426700 | A0A072VA73 | 97.955 | 538 | 11 | 0 | 1 | 538 | 1 | 538 | 0.0 | 1099 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0218470 | Msa1426700 | 0.853276 | 2.659844e-61 | -8.615850e-47 |
| Msa0218580 | Msa1426700 | 0.858761 | 6.599456e-63 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1426700 | MtrunA17_Chr2g0320631 | 97.955 | 538 | 11 | 0 | 1 | 538 | 1 | 538 | 0.0 | 1099 |
| Msa1426700 | MtrunA17_Chr4g0027671 | 76.580 | 538 | 117 | 4 | 9 | 539 | 8 | 543 | 0.0 | 841 |
| Msa1426700 | MtrunA17_Chr1g0179611 | 69.606 | 533 | 141 | 6 | 7 | 529 | 11 | 532 | 0.0 | 776 |
| Msa1426700 | MtrunA17_Chr5g0407121 | 48.713 | 505 | 179 | 4 | 19 | 522 | 17 | 442 | 1.54e-170 | 489 |
| Msa1426700 | MtrunA17_Chr8g0370321 | 47.817 | 504 | 181 | 5 | 19 | 522 | 17 | 438 | 1.55e-166 | 479 |
| Msa1426700 | MtrunA17_Chr4g0058241 | 37.342 | 158 | 92 | 4 | 25 | 182 | 84 | 234 | 2.07e-26 | 111 |
| Msa1426700 | MtrunA17_Chr4g0058241 | 29.146 | 199 | 106 | 6 | 296 | 477 | 243 | 423 | 9.37e-15 | 76.6 |
| Msa1426700 | MtrunA17_Chr3g0116701 | 36.943 | 157 | 92 | 4 | 25 | 181 | 80 | 229 | 4.52e-25 | 107 |
| Msa1426700 | MtrunA17_Chr3g0116701 | 30.189 | 212 | 110 | 9 | 284 | 477 | 228 | 419 | 1.25e-18 | 88.6 |
| Msa1426700 | MtrunA17_Chr3g0139371 | 36.420 | 162 | 96 | 4 | 20 | 181 | 55 | 209 | 1.78e-24 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1426700 | AT3G53030.1 | 70.155 | 516 | 139 | 3 | 7 | 522 | 9 | 509 | 0.0 | 753 |
| Msa1426700 | AT5G22840.1 | 69.962 | 526 | 138 | 4 | 7 | 527 | 12 | 522 | 0.0 | 742 |
| Msa1426700 | AT3G44850.1 | 70.709 | 536 | 137 | 6 | 1 | 523 | 1 | 529 | 0.0 | 740 |
| Msa1426700 | AT4G35500.1 | 49.163 | 478 | 176 | 5 | 21 | 492 | 19 | 435 | 6.26e-166 | 478 |
| Msa1426700 | AT4G35500.2 | 47.182 | 479 | 185 | 6 | 21 | 492 | 19 | 436 | 1.26e-156 | 454 |
| Msa1426700 | AT2G17530.2 | 63.483 | 178 | 65 | 0 | 21 | 198 | 19 | 196 | 1.28e-78 | 251 |
| Msa1426700 | AT2G17530.2 | 47.500 | 160 | 76 | 2 | 253 | 404 | 183 | 342 | 9.54e-40 | 148 |
| Msa1426700 | AT2G17530.1 | 44.484 | 281 | 123 | 3 | 253 | 525 | 183 | 438 | 2.69e-77 | 250 |
| Msa1426700 | AT2G17530.1 | 60.417 | 192 | 73 | 1 | 21 | 212 | 19 | 207 | 3.03e-77 | 250 |
| Msa1426700 | AT2G17530.3 | 44.484 | 281 | 123 | 3 | 253 | 525 | 183 | 438 | 2.69e-77 | 250 |
Find 165 sgRNAs with CRISPR-Local
Find 396 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCTCGAATTCAGTGAA+TGG | 0.506638 | tig0026063:+20552 | None:intergenic |
| AAACATGATAAAAGTTCGAA+TGG | 0.554779 | tig0026063:-18899 | Msa1426700:CDS |
| AAACCTCGAATTCAGTGAAT+GGG | 0.509572 | tig0026063:+20553 | None:intergenic |
| AAAGAACGAGCAGGAGGCTG+TGG | 0.520764 | tig0026063:-15682 | Msa1426700:CDS |
| AAATTTCTTTGACCATGGGA+AGG | 0.567519 | tig0026063:+19107 | None:intergenic |
| AAGGGAAAAGAACGAGCAGG+AGG | 0.644457 | tig0026063:-15688 | Msa1426700:CDS |
| AAGTCCCGAGGCAGCAGAGG+AGG | 0.531648 | tig0026063:-18813 | Msa1426700:CDS |
| AAGTGCAAGCTTGTGGATTT+TGG | 0.224760 | tig0026063:-18590 | Msa1426700:CDS |
| AATCCAAACCCACCAAAACA+TGG | 0.629227 | tig0026063:+19080 | None:intergenic |
| AATTGCTGAAGGGGATCTAG+AGG | 0.602637 | tig0026063:-19266 | Msa1426700:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCAGTGAATGGGAAAATTA+GGG | + | tig0026063:15203-15222 | None:intergenic | 30.0% | |
| ATTCAGTGAATGGGAAAATT+AGG | + | tig0026063:15204-15223 | None:intergenic | 30.0% | |
| TATTTGAAGAATAATGGTGG+TGG | + | tig0026063:15275-15294 | None:intergenic | 30.0% | |
| TTCAGTGTAATTACAAGTGT+CGG | - | tig0026063:16067-16086 | Msa1426700:intron | 30.0% | |
| GAAACTAGAAAGAGAATCAA+GGG | + | tig0026063:16242-16261 | None:intergenic | 30.0% | |
| AGAAACTAGAAAGAGAATCA+AGG | + | tig0026063:16243-16262 | None:intergenic | 30.0% | |
| TGAAAACTCTACTAACAATG+GGG | - | tig0026063:16378-16397 | Msa1426700:intron | 30.0% | |
| ATTCTCAAACAAATTGCTGA+AGG | - | tig0026063:16486-16505 | Msa1426700:intron | 30.0% | |
| TTCTCAAACAAATTGCTGAA+GGG | - | tig0026063:16487-16506 | Msa1426700:intron | 30.0% | |
| ATCAAGTATAGTGATTATCG+TGG | - | tig0026063:16627-16646 | Msa1426700:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0026063 | gene | 15151 | 20634 | 15151 | ID=Msa1426700;Name=Msa1426700 |
| tig0026063 | mRNA | 15151 | 20634 | 15151 | ID=Msa1426700-mRNA-1;Parent=Msa1426700;Name=Msa1426700-mRNA-1;_AED=0.03;_eAED=0.03;_QI=217|1|1|1|1|1|4|451|539 |
| tig0026063 | exon | 15151 | 16012 | 15151 | ID=Msa1426700-mRNA-1:exon:5075;Parent=Msa1426700-mRNA-1 |
| tig0026063 | exon | 16282 | 16329 | 16282 | ID=Msa1426700-mRNA-1:exon:5074;Parent=Msa1426700-mRNA-1 |
| tig0026063 | exon | 18397 | 19368 | 18397 | ID=Msa1426700-mRNA-1:exon:5073;Parent=Msa1426700-mRNA-1 |
| tig0026063 | exon | 20229 | 20634 | 20229 | ID=Msa1426700-mRNA-1:exon:5072;Parent=Msa1426700-mRNA-1 |
| tig0026063 | five_prime_UTR | 20418 | 20634 | 20418 | ID=Msa1426700-mRNA-1:five_prime_utr;Parent=Msa1426700-mRNA-1 |
| tig0026063 | CDS | 20229 | 20417 | 20229 | ID=Msa1426700-mRNA-1:cds;Parent=Msa1426700-mRNA-1 |
| tig0026063 | CDS | 18397 | 19368 | 18397 | ID=Msa1426700-mRNA-1:cds;Parent=Msa1426700-mRNA-1 |
| tig0026063 | CDS | 16282 | 16329 | 16282 | ID=Msa1426700-mRNA-1:cds;Parent=Msa1426700-mRNA-1 |
| tig0026063 | CDS | 15602 | 16012 | 15602 | ID=Msa1426700-mRNA-1:cds;Parent=Msa1426700-mRNA-1 |
| tig0026063 | three_prime_UTR | 15151 | 15601 | 15151 | ID=Msa1426700-mRNA-1:three_prime_utr;Parent=Msa1426700-mRNA-1 |
| Gene Sequence |
>Msa1426700 ATGGCGGAGGATCGCAAAGATGCGAGTGATTACAGTTCGGAAGATGAAGGAACTGAGGATTACAAACGAGGTGGTTATCATGCGGTTCAGATTGGTGACACTTTCAAAAATGGGTGCTATGTTGTTCAGAGTAAGCTTGGCTGGGGTCATTTTTCAACTGTTTGGCTCGCTTGGGATACTCTCAAATTGCGATATGTTGCCTTAAAAATTCAGAAGAGTGCTCAGCACTACACGGAAGCGGCAATGGATGAAATAAAGATTCTCAAACAAATTGCTGAAGGGGATCTAGAGGATAAGAAATGTGTTGTGAAGCTTTTGGACCACTTTAAGCATTCAGGGCCTAATGGTCAGCATGTTTGTATGGTTTTTGAATTCCTTGGGGACAATCTTCTTACACTTATCAAGTATAGTGATTATCGTGGTATTCCCCTTCCCATGGTCAAAGAAATTTGCTACCATGTTTTGGTGGGTTTGGATTATTTGCATCGTGAGCTTTCTATAATACACACTGATTTGAAGCCAGAAAATGTTTTGCTTGTCTCTCCAATAGATCCATCTAAAGATCCTAGAAAATCAGGAGTCCCACTTATACTTCCAACCACTAAAGTCAAGACTGTATCCAAGAACGGGACCACAAAACATGATAAAAGTTCGAATGGAGATTTAACCAAGAACCAGAAAAAGAAGATGCGGAAAAAGGCTAAAAAAGCAGCTCAGAAGGAAAGTCCCGAGGCAGCAGAGGAGGATTCTGAAGCACCTGAACAAGATGATTCTAGTAATGATGTGAAACCAATTGTAGAAAATGGTGAAGGTAAACCTAATAGTCCCACAAGTAAAGATGAATCAGCAATGACTTCTGAAACTAAGGATGTTCCACAAGGAAGTCAGGTTTCTAGGAGAGGTAGTTGCTCTACGAGAAAGAAGTTGCTCGCAGCTGCTGATCTTAAGTGCAAGCTTGTGGATTTTGGAAATGCTTGTTGGACGTATAAACAATTTACAAATGATATCCAGACTAGGCAGTATAGATGTCCTGAGGTTCTTCTAGGTTCTAAATACTCGACTCCGGCAGATATGTGGTCCTTTGCTTGCATATGCTTTGAGCTTGCCACTGGCGATGTTCTTTTTGATCCTCACAGTGGTGAAAACTATGACAGGGATGAGGATCATCTGGCATTGATGATGGAGCTTCTCGGGGTGATGCCTCGCAAGATTGCTCTTGGCGGCCGCTATTCCCGGGACTTTTTCAATAGATCTGGTGATTTGAGACACATTCGCCGGTTGCGGTTCTGGCCTATTACTAAGGTTTTGACGGAGAAGTATGATTTCAGTGCGCAAGATGCAAGTGACATGTCTGACTTCCTTGTTCCGTTACTTGACTTTGTTCCTGAAAAGAGACCAACGGCCGCTCAGTGTCTTACTCATCCATGGATGAGTGCAGGTCCTCGGACTCTTGAGCCCTCAGTGAATAGCATCCATGCTGATGCTATTAACGGAGAAAAGCCTGGAAAGTTAAGGGAAAAGAACGAGCAGGAGGCTGTGGAAGTTAGTATGGGGAATATGACCATTGATGGAACTCCAAAGACACTCAAAGATTTCCAATCTACAAAACCATCGGCGTAG |
| Protein sequence |
>Msa1426700 MAEDRKDASDYSSEDEGTEDYKRGGYHAVQIGDTFKNGCYVVQSKLGWGHFSTVWLAWDTLKLRYVALKIQKSAQHYTEAAMDEIKILKQIAEGDLEDKKCVVKLLDHFKHSGPNGQHVCMVFEFLGDNLLTLIKYSDYRGIPLPMVKEICYHVLVGLDYLHRELSIIHTDLKPENVLLVSPIDPSKDPRKSGVPLILPTTKVKTVSKNGTTKHDKSSNGDLTKNQKKKMRKKAKKAAQKESPEAAEEDSEAPEQDDSSNDVKPIVENGEGKPNSPTSKDESAMTSETKDVPQGSQVSRRGSCSTRKKLLAAADLKCKLVDFGNACWTYKQFTNDIQTRQYRCPEVLLGSKYSTPADMWSFACICFELATGDVLFDPHSGENYDRDEDHLALMMELLGVMPRKIALGGRYSRDFFNRSGDLRHIRRLRFWPITKVLTEKYDFSAQDASDMSDFLVPLLDFVPEKRPTAAQCLTHPWMSAGPRTLEPSVNSIHADAINGEKPGKLREKNEQEAVEVSMGNMTIDGTPKTLKDFQSTKPSA* |
