Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1430140 | XP_024627814.1 | 77.907 | 258 | 37 | 2 | 1 | 258 | 1 | 238 | 4.04e-138 | 398 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1430140 | A0A396GR48 | 77.907 | 258 | 37 | 2 | 1 | 258 | 1 | 238 | 1.93e-138 | 398 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0035020 | Msa1430140 | 0.801164 | 1.024362e-48 | -8.615850e-47 |
| Msa1430140 | Msa1460760 | 0.993250 | 1.879308e-198 | -8.615850e-47 |
| Msa1069190 | Msa1430140 | 0.807671 | 4.510118e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1430140 | MtrunA17_Chr8g0390051 | 77.907 | 258 | 37 | 2 | 1 | 258 | 1 | 238 | 3.72e-142 | 398 |
| Msa1430140 | MtrunA17_Chr8g0390061 | 61.538 | 247 | 54 | 4 | 1 | 247 | 1 | 206 | 5.55e-97 | 282 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAGAAATGTTTATACTTC+AGG | 0.257939 | tig0026637:-34438 | Msa1430140:CDS |
| TAATGATGTTGAAATGAATT+TGG | 0.258979 | tig0026637:-34541 | Msa1430140:CDS |
| ACTACTCATGTTTATACTTC+AGG | 0.281761 | tig0026637:-34468 | Msa1430140:CDS |
| ATTGAAGTTGATAATAGTTT+TGG | 0.297080 | tig0026637:-35105 | Msa1430140:CDS |
| AAGAGTGATGACAAAGAAAA+AGG | 0.308555 | tig0026637:-34894 | Msa1430140:CDS |
| CAAAGCTTTCATGATGAAAA+TGG | 0.312117 | tig0026637:-34690 | Msa1430140:CDS |
| GTTTCCCTTCTCTTTAATTC+CGG | 0.323029 | tig0026637:+34776 | None:intergenic |
| GAAATGGAGTATGAATCTTT+TGG | 0.345782 | tig0026637:-35167 | Msa1430140:CDS |
| TCTTGTATTAATTTCTTCAT+CGG | 0.409976 | tig0026637:+34494 | None:intergenic |
| ACCAAGCTTGTTGTAGGAAG+TGG | 0.446868 | tig0026637:-35020 | Msa1430140:CDS |
| AAAAGGATCTAACATAAAAC+AGG | 0.452318 | tig0026637:-35129 | Msa1430140:CDS |
| CTTCCTACAACAAGCTTGGT+TGG | 0.453707 | tig0026637:+35023 | None:intergenic |
| GACAAGGATGTTGAAGAAAC+CGG | 0.472516 | tig0026637:-34795 | Msa1430140:CDS |
| AATGTTTATAAAGTCACATA+TGG | 0.478201 | tig0026637:-34570 | Msa1430140:CDS |
| AGTCCAACCAAGCTTGTTGT+AGG | 0.489137 | tig0026637:-35026 | Msa1430140:CDS |
| GAAATCAAAATTCTCATTGA+AGG | 0.492366 | tig0026637:-34823 | Msa1430140:CDS |
| GGAAGGTACCAAAAGCCAAA+AGG | 0.513006 | tig0026637:-35146 | Msa1430140:CDS |
| TCCACTTCCTACAACAAGCT+TGG | 0.517256 | tig0026637:+35019 | None:intergenic |
| GATGTTGAAATGAATTTGGA+GGG | 0.522379 | tig0026637:-34537 | Msa1430140:CDS |
| AAAATTCTCATTGAAGGAGA+AGG | 0.537516 | tig0026637:-34817 | Msa1430140:CDS |
| TCAGGAAAGGTAGATTCTGT+TGG | 0.537763 | tig0026637:-34936 | Msa1430140:CDS |
| CTGCAAAATGATATGCATGT+TGG | 0.552120 | tig0026637:-34855 | Msa1430140:CDS |
| GTTTGCAATCAAGAAATAAG+TGG | 0.553693 | tig0026637:-34416 | Msa1430140:CDS |
| GAAACCGGAATTAAAGAGAA+GGG | 0.561260 | tig0026637:-34780 | Msa1430140:CDS |
| TGATGTTGAAATGAATTTGG+AGG | 0.570754 | tig0026637:-34538 | Msa1430140:CDS |
| AGAAACCGGAATTAAAGAGA+AGG | 0.597087 | tig0026637:-34781 | Msa1430140:CDS |
| CTCATTGAAGGAGAAGGACA+AGG | 0.599240 | tig0026637:-34811 | Msa1430140:CDS |
| GAAAGGGATAATGTCGCGAT+CGG | 0.615835 | tig0026637:-34979 | Msa1430140:CDS |
| GAGTGGCGCAAATGATGACG+AGG | 0.674631 | tig0026637:-34727 | Msa1430140:CDS |
| TGATGACGAGGAAACAAGTG+AGG | 0.692705 | tig0026637:-34715 | Msa1430140:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGAAGTTGATAATAGTTT+TGG | - | tig0026637:34469-34488 | Msa1430140:CDS | 20.0% |
| !! | AATGTTTATAAAGTCACATA+TGG | - | tig0026637:35004-35023 | Msa1430140:CDS | 20.0% |
| !! | TAATGATGTTGAAATGAATT+TGG | - | tig0026637:35033-35052 | Msa1430140:CDS | 20.0% |
| !! | TCTTGTATTAATTTCTTCAT+CGG | + | tig0026637:35083-35102 | None:intergenic | 20.0% |
| !!! | ATTGAAGTTGATAATAGTTT+TGG | - | tig0026637:34469-34488 | Msa1430140:CDS | 20.0% |
| !! | AATGTTTATAAAGTCACATA+TGG | - | tig0026637:35004-35023 | Msa1430140:CDS | 20.0% |
| !! | TAATGATGTTGAAATGAATT+TGG | - | tig0026637:35033-35052 | Msa1430140:CDS | 20.0% |
| !! | TCTTGTATTAATTTCTTCAT+CGG | + | tig0026637:35083-35102 | None:intergenic | 20.0% |
| !! | TGTTTTATGTTAGATCCTTT+TGG | + | tig0026637:34446-34465 | None:intergenic | 25.0% |
| ! | AAAAGGATCTAACATAAAAC+AGG | - | tig0026637:34445-34464 | Msa1430140:CDS | 25.0% |
| ! | GAAATCAAAATTCTCATTGA+AGG | - | tig0026637:34751-34770 | Msa1430140:CDS | 25.0% |
| !! | AATGTTTTCTTCAGTAAAGT+CGG | + | tig0026637:34918-34937 | None:intergenic | 25.0% |
| !! | CAACTTTTAGCAAAGTAAAT+TGG | - | tig0026637:34939-34958 | Msa1430140:CDS | 25.0% |
| ! | TCAAGAAATGTTTATACTTC+AGG | - | tig0026637:35136-35155 | Msa1430140:CDS | 25.0% |
| !! | TGTTTTATGTTAGATCCTTT+TGG | + | tig0026637:34446-34465 | None:intergenic | 25.0% |
| ! | AAAAGGATCTAACATAAAAC+AGG | - | tig0026637:34445-34464 | Msa1430140:CDS | 25.0% |
| ! | GAAATCAAAATTCTCATTGA+AGG | - | tig0026637:34751-34770 | Msa1430140:CDS | 25.0% |
| !! | AATGTTTTCTTCAGTAAAGT+CGG | + | tig0026637:34918-34937 | None:intergenic | 25.0% |
| !! | CAACTTTTAGCAAAGTAAAT+TGG | - | tig0026637:34939-34958 | Msa1430140:CDS | 25.0% |
| ! | TCAAGAAATGTTTATACTTC+AGG | - | tig0026637:35136-35155 | Msa1430140:CDS | 25.0% |
| CACACAGAAACAAAAAACTA+TGG | - | tig0026637:34524-34543 | Msa1430140:CDS | 30.0% | |
| !!! | CTAATTTTTTTGGACGGAAA+GGG | - | tig0026637:34579-34598 | Msa1430140:CDS | 30.0% |
| AAGAGTGATGACAAAGAAAA+AGG | - | tig0026637:34680-34699 | Msa1430140:CDS | 30.0% | |
| AAAATTCTCATTGAAGGAGA+AGG | - | tig0026637:34757-34776 | Msa1430140:CDS | 30.0% | |
| CAAAGCTTTCATGATGAAAA+TGG | - | tig0026637:34884-34903 | Msa1430140:CDS | 30.0% | |
| ! | ATGTTTTCTTCAGTAAAGTC+GGG | + | tig0026637:34917-34936 | None:intergenic | 30.0% |
| TGATGTTGAAATGAATTTGG+AGG | - | tig0026637:35036-35055 | Msa1430140:CDS | 30.0% | |
| GATGTTGAAATGAATTTGGA+GGG | - | tig0026637:35037-35056 | Msa1430140:CDS | 30.0% | |
| ACTACTCATGTTTATACTTC+AGG | - | tig0026637:35106-35125 | Msa1430140:CDS | 30.0% | |
| GTTTGCAATCAAGAAATAAG+TGG | - | tig0026637:35158-35177 | Msa1430140:CDS | 30.0% | |
| CACACAGAAACAAAAAACTA+TGG | - | tig0026637:34524-34543 | Msa1430140:CDS | 30.0% | |
| !!! | CTAATTTTTTTGGACGGAAA+GGG | - | tig0026637:34579-34598 | Msa1430140:CDS | 30.0% |
| AAGAGTGATGACAAAGAAAA+AGG | - | tig0026637:34680-34699 | Msa1430140:CDS | 30.0% | |
| AAAATTCTCATTGAAGGAGA+AGG | - | tig0026637:34757-34776 | Msa1430140:CDS | 30.0% | |
| CAAAGCTTTCATGATGAAAA+TGG | - | tig0026637:34884-34903 | Msa1430140:CDS | 30.0% | |
| ! | ATGTTTTCTTCAGTAAAGTC+GGG | + | tig0026637:34917-34936 | None:intergenic | 30.0% |
| TGATGTTGAAATGAATTTGG+AGG | - | tig0026637:35036-35055 | Msa1430140:CDS | 30.0% | |
| GATGTTGAAATGAATTTGGA+GGG | - | tig0026637:35037-35056 | Msa1430140:CDS | 30.0% | |
| ACTACTCATGTTTATACTTC+AGG | - | tig0026637:35106-35125 | Msa1430140:CDS | 30.0% | |
| GTTTGCAATCAAGAAATAAG+TGG | - | tig0026637:35158-35177 | Msa1430140:CDS | 30.0% | |
| ! | TGGAGTATGAATCTTTTGGA+AGG | - | tig0026637:34411-34430 | Msa1430140:CDS | 35.0% |
| !!! | TGTTAGATCCTTTTGGCTTT+TGG | + | tig0026637:34439-34458 | None:intergenic | 35.0% |
| !!! | TAGTTTTGGCTATTTGTGTC+TGG | - | tig0026637:34483-34502 | Msa1430140:CDS | 35.0% |
| !!! | GGAAGTGGAGCTAATTTTTT+TGG | - | tig0026637:34569-34588 | Msa1430140:CDS | 35.0% |
| !!! | GTGGAGCTAATTTTTTTGGA+CGG | - | tig0026637:34573-34592 | Msa1430140:CDS | 35.0% |
| !!! | GCTAATTTTTTTGGACGGAA+AGG | - | tig0026637:34578-34597 | Msa1430140:CDS | 35.0% |
| ! | ATCGGATGAAAGAGCTTTTT+CGG | - | tig0026637:34613-34632 | Msa1430140:CDS | 35.0% |
| ! | AAGAAAAAGGAGTACAACTC+AGG | - | tig0026637:34693-34712 | Msa1430140:CDS | 35.0% |
| ! | AGAAAAAGGAGTACAACTCA+GGG | - | tig0026637:34694-34713 | Msa1430140:CDS | 35.0% |
| CTGCAAAATGATATGCATGT+TGG | - | tig0026637:34719-34738 | Msa1430140:CDS | 35.0% | |
| AGAAACCGGAATTAAAGAGA+AGG | - | tig0026637:34793-34812 | Msa1430140:CDS | 35.0% | |
| GAAACCGGAATTAAAGAGAA+GGG | - | tig0026637:34794-34813 | Msa1430140:CDS | 35.0% | |
| GTTTCCCTTCTCTTTAATTC+CGG | + | tig0026637:34801-34820 | None:intergenic | 35.0% | |
| CAACAAGATGCAGAAAAATG+TGG | - | tig0026637:34820-34839 | Msa1430140:CDS | 35.0% | |
| ! | TGGAGTATGAATCTTTTGGA+AGG | - | tig0026637:34411-34430 | Msa1430140:CDS | 35.0% |
| !!! | TGTTAGATCCTTTTGGCTTT+TGG | + | tig0026637:34439-34458 | None:intergenic | 35.0% |
| !!! | TAGTTTTGGCTATTTGTGTC+TGG | - | tig0026637:34483-34502 | Msa1430140:CDS | 35.0% |
| !!! | GGAAGTGGAGCTAATTTTTT+TGG | - | tig0026637:34569-34588 | Msa1430140:CDS | 35.0% |
| !!! | GTGGAGCTAATTTTTTTGGA+CGG | - | tig0026637:34573-34592 | Msa1430140:CDS | 35.0% |
| !!! | GCTAATTTTTTTGGACGGAA+AGG | - | tig0026637:34578-34597 | Msa1430140:CDS | 35.0% |
| ! | ATCGGATGAAAGAGCTTTTT+CGG | - | tig0026637:34613-34632 | Msa1430140:CDS | 35.0% |
| ! | AAGAAAAAGGAGTACAACTC+AGG | - | tig0026637:34693-34712 | Msa1430140:CDS | 35.0% |
| ! | AGAAAAAGGAGTACAACTCA+GGG | - | tig0026637:34694-34713 | Msa1430140:CDS | 35.0% |
| CTGCAAAATGATATGCATGT+TGG | - | tig0026637:34719-34738 | Msa1430140:CDS | 35.0% | |
| AGAAACCGGAATTAAAGAGA+AGG | - | tig0026637:34793-34812 | Msa1430140:CDS | 35.0% | |
| GAAACCGGAATTAAAGAGAA+GGG | - | tig0026637:34794-34813 | Msa1430140:CDS | 35.0% | |
| GTTTCCCTTCTCTTTAATTC+CGG | + | tig0026637:34801-34820 | None:intergenic | 35.0% | |
| CAACAAGATGCAGAAAAATG+TGG | - | tig0026637:34820-34839 | Msa1430140:CDS | 35.0% | |
| TCAGGAAAGGTAGATTCTGT+TGG | - | tig0026637:34638-34657 | Msa1430140:CDS | 40.0% | |
| GACAAGGATGTTGAAGAAAC+CGG | - | tig0026637:34779-34798 | Msa1430140:CDS | 40.0% | |
| CAAGATGCAGAAAAATGTGG+TGG | - | tig0026637:34823-34842 | Msa1430140:CDS | 40.0% | |
| ! | TTTTCTTCAGTAAAGTCGGG+CGG | + | tig0026637:34914-34933 | None:intergenic | 40.0% |
| TCAGGAAAGGTAGATTCTGT+TGG | - | tig0026637:34638-34657 | Msa1430140:CDS | 40.0% | |
| GACAAGGATGTTGAAGAAAC+CGG | - | tig0026637:34779-34798 | Msa1430140:CDS | 40.0% | |
| CAAGATGCAGAAAAATGTGG+TGG | - | tig0026637:34823-34842 | Msa1430140:CDS | 40.0% | |
| ! | TTTTCTTCAGTAAAGTCGGG+CGG | + | tig0026637:34914-34933 | None:intergenic | 40.0% |
| !! | GGAAGGTACCAAAAGCCAAA+AGG | - | tig0026637:34428-34447 | Msa1430140:CDS | 45.0% |
| !! | AGTCCAACCAAGCTTGTTGT+AGG | - | tig0026637:34548-34567 | Msa1430140:CDS | 45.0% |
| CTTCCTACAACAAGCTTGGT+TGG | + | tig0026637:34554-34573 | None:intergenic | 45.0% | |
| !! | ACCAAGCTTGTTGTAGGAAG+TGG | - | tig0026637:34554-34573 | Msa1430140:CDS | 45.0% |
| TCCACTTCCTACAACAAGCT+TGG | + | tig0026637:34558-34577 | None:intergenic | 45.0% | |
| GAAAGGGATAATGTCGCGAT+CGG | - | tig0026637:34595-34614 | Msa1430140:CDS | 45.0% | |
| !!! | GAAAGAGCTTTTTCGGACTC+AGG | - | tig0026637:34620-34639 | Msa1430140:CDS | 45.0% |
| !!! | AGCTTTTTCGGACTCAGGAA+AGG | - | tig0026637:34625-34644 | Msa1430140:CDS | 45.0% |
| CTCATTGAAGGAGAAGGACA+AGG | - | tig0026637:34763-34782 | Msa1430140:CDS | 45.0% | |
| ! | CAGAAAAATGTGGTGGAGAG+TGG | - | tig0026637:34830-34849 | Msa1430140:CDS | 45.0% |
| ! | TGATGACGAGGAAACAAGTG+AGG | - | tig0026637:34859-34878 | Msa1430140:CDS | 45.0% |
| !! | GGAAGGTACCAAAAGCCAAA+AGG | - | tig0026637:34428-34447 | Msa1430140:CDS | 45.0% |
| !! | AGTCCAACCAAGCTTGTTGT+AGG | - | tig0026637:34548-34567 | Msa1430140:CDS | 45.0% |
| CTTCCTACAACAAGCTTGGT+TGG | + | tig0026637:34554-34573 | None:intergenic | 45.0% | |
| !! | ACCAAGCTTGTTGTAGGAAG+TGG | - | tig0026637:34554-34573 | Msa1430140:CDS | 45.0% |
| TCCACTTCCTACAACAAGCT+TGG | + | tig0026637:34558-34577 | None:intergenic | 45.0% | |
| GAAAGGGATAATGTCGCGAT+CGG | - | tig0026637:34595-34614 | Msa1430140:CDS | 45.0% | |
| !!! | GAAAGAGCTTTTTCGGACTC+AGG | - | tig0026637:34620-34639 | Msa1430140:CDS | 45.0% |
| !!! | AGCTTTTTCGGACTCAGGAA+AGG | - | tig0026637:34625-34644 | Msa1430140:CDS | 45.0% |
| CTCATTGAAGGAGAAGGACA+AGG | - | tig0026637:34763-34782 | Msa1430140:CDS | 45.0% | |
| ! | CAGAAAAATGTGGTGGAGAG+TGG | - | tig0026637:34830-34849 | Msa1430140:CDS | 45.0% |
| ! | TGATGACGAGGAAACAAGTG+AGG | - | tig0026637:34859-34878 | Msa1430140:CDS | 45.0% |
| ! | GAGTGGCGCAAATGATGACG+AGG | - | tig0026637:34847-34866 | Msa1430140:CDS | 55.0% |
| ! | GAGTGGCGCAAATGATGACG+AGG | - | tig0026637:34847-34866 | Msa1430140:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0026637 | gene | 34410 | 35186 | 34410 | ID=Msa1430140;Name=Msa1430140 |
| tig0026637 | mRNA | 34410 | 35186 | 34410 | ID=Msa1430140-mRNA-1;Parent=Msa1430140;Name=Msa1430140-mRNA-1;_AED=0.28;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|258 |
| tig0026637 | exon | 34410 | 35186 | 34410 | ID=Msa1430140-mRNA-1:exon:6849;Parent=Msa1430140-mRNA-1 |
| tig0026637 | CDS | 34410 | 35186 | 34410 | ID=Msa1430140-mRNA-1:cds;Parent=Msa1430140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
