Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1430430 | AES71835.1 | 93.939 | 66 | 3 | 1 | 1 | 66 | 1 | 65 | 8.03e-34 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1430430 | G7J9J6 | 93.939 | 66 | 3 | 1 | 1 | 66 | 1 | 65 | 3.84e-34 | 120 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0421170 | Msa1430430 | 0.908682 | 1.308800e-81 | -8.615850e-47 |
| Msa0513610 | Msa1430430 | 0.910251 | 2.309129e-82 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1430430 | MtrunA17_Chr3g0121081 | 93.939 | 66 | 3 | 1 | 1 | 66 | 1 | 65 | 7.39e-38 | 120 |
| Msa1430430 | MtrunA17_Chr2g0281351 | 73.333 | 60 | 12 | 2 | 1 | 60 | 1 | 56 | 5.53e-22 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCAACGGTGGAGTATT+AGG | 0.287872 | tig0026659:+53 | Msa1430430:CDS |
| ACCTGACCCCGCTTTGGAAT+CGG | 0.376203 | tig0026659:-95 | None:intergenic |
| AGAAGGAGCAGCACATGATA+TGG | 0.377907 | tig0026659:-174 | None:intergenic |
| TCCGATTCCAAAGCGGGGTC+AGG | 0.419316 | tig0026659:+94 | Msa1430430:CDS |
| ATCACCACCTGACCCCGCTT+TGG | 0.431061 | tig0026659:-101 | None:intergenic |
| AAGCGGGGTCAGGTGGTGAT+TGG | 0.451258 | tig0026659:+104 | Msa1430430:CDS |
| TGACCGTCCGATTCCAAAGC+GGG | 0.457454 | tig0026659:+88 | Msa1430430:CDS |
| GTGGTGATTGGAATTGTGTT+GGG | 0.460629 | tig0026659:+116 | Msa1430430:CDS |
| TTACAAATTAAGCTAAAAGC+TGG | 0.468928 | tig0026659:-205 | None:intergenic |
| GATTGGAATTGTGTTGGGAT+TGG | 0.472815 | tig0026659:+121 | Msa1430430:CDS |
| GGTGGTGATTGGAATTGTGT+TGG | 0.485203 | tig0026659:+115 | Msa1430430:CDS |
| AAAAGCTGGACTCTAGAAGA+AGG | 0.550483 | tig0026659:-191 | None:intergenic |
| TGTGTTGGGATTGGCCAACT+CGG | 0.572810 | tig0026659:+130 | Msa1430430:CDS |
| GAAGATGCAAACAACCGAGT+TGG | 0.598645 | tig0026659:-144 | None:intergenic |
| TCAACGGTGGAGTATTAGGA+AGG | 0.608387 | tig0026659:+57 | Msa1430430:CDS |
| GATTCCAAAGCGGGGTCAGG+TGG | 0.610196 | tig0026659:+97 | Msa1430430:CDS |
| CAAAGCAAAGAGAAGATGGA+GGG | 0.621656 | tig0026659:+5 | None:intergenic |
| ACTGCAATCATGAAAATCAA+CGG | 0.647400 | tig0026659:+41 | Msa1430430:CDS |
| GACCCCGCTTTGGAATCGGA+CGG | 0.688444 | tig0026659:-91 | None:intergenic |
| GCAATCATGAAAATCAACGG+TGG | 0.699924 | tig0026659:+44 | Msa1430430:CDS |
| GACCGTCCGATTCCAAAGCG+GGG | 0.710779 | tig0026659:+89 | Msa1430430:CDS |
| GTGACCGTCCGATTCCAAAG+CGG | 0.721311 | tig0026659:+87 | Msa1430430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTGCAATCATGAAAATCAA+CGG | + | tig0026659:41-60 | Msa1430430:CDS | 30.0% | |
| ! | AAAATCAACGGTGGAGTATT+AGG | + | tig0026659:53-72 | Msa1430430:CDS | 35.0% |
| GCAATCATGAAAATCAACGG+TGG | + | tig0026659:44-63 | Msa1430430:CDS | 40.0% | |
| GTGGTGATTGGAATTGTGTT+GGG | + | tig0026659:116-135 | Msa1430430:CDS | 40.0% | |
| GATTGGAATTGTGTTGGGAT+TGG | + | tig0026659:121-140 | Msa1430430:CDS | 40.0% | |
| ! | AAAAGCTGGACTCTAGAAGA+AGG | - | tig0026659:194-213 | None:intergenic | 40.0% |
| ! | TCAACGGTGGAGTATTAGGA+AGG | + | tig0026659:57-76 | Msa1430430:CDS | 45.0% |
| GGTGGTGATTGGAATTGTGT+TGG | + | tig0026659:115-134 | Msa1430430:CDS | 45.0% | |
| GAAGATGCAAACAACCGAGT+TGG | - | tig0026659:147-166 | None:intergenic | 45.0% | |
| AGAAGGAGCAGCACATGATA+TGG | - | tig0026659:177-196 | None:intergenic | 45.0% | |
| !! | TGTGTTGGGATTGGCCAACT+CGG | + | tig0026659:130-149 | Msa1430430:CDS | 50.0% |
| GTGACCGTCCGATTCCAAAG+CGG | + | tig0026659:87-106 | Msa1430430:CDS | 55.0% | |
| TGACCGTCCGATTCCAAAGC+GGG | + | tig0026659:88-107 | Msa1430430:CDS | 55.0% | |
| !! | ACCTGACCCCGCTTTGGAAT+CGG | - | tig0026659:98-117 | None:intergenic | 55.0% |
| GACCGTCCGATTCCAAAGCG+GGG | + | tig0026659:89-108 | Msa1430430:CDS | 60.0% | |
| !! | GACCCCGCTTTGGAATCGGA+CGG | - | tig0026659:94-113 | None:intergenic | 60.0% |
| TCCGATTCCAAAGCGGGGTC+AGG | + | tig0026659:94-113 | Msa1430430:CDS | 60.0% | |
| GATTCCAAAGCGGGGTCAGG+TGG | + | tig0026659:97-116 | Msa1430430:CDS | 60.0% | |
| ATCACCACCTGACCCCGCTT+TGG | - | tig0026659:104-123 | None:intergenic | 60.0% | |
| AAGCGGGGTCAGGTGGTGAT+TGG | + | tig0026659:104-123 | Msa1430430:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0026659 | gene | 20 | 220 | 20 | ID=Msa1430430;Name=Msa1430430 |
| tig0026659 | mRNA | 20 | 220 | 20 | ID=Msa1430430-mRNA-1;Parent=Msa1430430;Name=Msa1430430-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|66 |
| tig0026659 | exon | 20 | 220 | 20 | ID=Msa1430430-mRNA-1:exon:7000;Parent=Msa1430430-mRNA-1 |
| tig0026659 | CDS | 20 | 220 | 20 | ID=Msa1430430-mRNA-1:cds;Parent=Msa1430430-mRNA-1 |
| Gene Sequence |
| Protein sequence |
