Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0421170 | AES71835.1 | 91.176 | 68 | 3 | 2 | 1 | 68 | 1 | 65 | 6.06e-32 | 115 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0421170 | G7J9J6 | 91.176 | 68 | 3 | 2 | 1 | 68 | 1 | 65 | 2.89e-32 | 115 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0032610 | Msa0421170 | 0.821050 | 4.961854e-53 | -8.615850e-47 |
| Msa0154770 | Msa0421170 | 0.827049 | 1.939044e-54 | -8.615850e-47 |
| Msa0162770 | Msa0421170 | -0.803114 | 4.068395e-49 | -8.615850e-47 |
| Msa0421170 | Msa0513610 | 0.854067 | 1.575506e-61 | -8.615850e-47 |
| Msa0421170 | Msa0553220 | -0.817478 | 3.230655e-52 | -8.615850e-47 |
| Msa0421170 | Msa1191470 | -0.802407 | 5.693610e-49 | -8.615850e-47 |
| Msa0421170 | Msa1322190 | 0.815824 | 7.584943e-52 | -8.615850e-47 |
| Msa0421170 | Msa1430430 | 0.908682 | 1.308800e-81 | -8.615850e-47 |
| Msa0421170 | Msa1459760 | -0.817530 | 3.144131e-52 | -8.615850e-47 |
| Msa0279210 | Msa0421170 | -0.811655 | 6.280491e-51 | -8.615850e-47 |
| Msa0318970 | Msa0421170 | -0.814400 | 1.570436e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0421170 | MtrunA17_Chr3g0121081 | 91.176 | 68 | 3 | 2 | 1 | 68 | 1 | 65 | 5.57e-36 | 115 |
| Msa0421170 | MtrunA17_Chr2g0281351 | 70.968 | 62 | 12 | 3 | 1 | 62 | 1 | 56 | 4.67e-20 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCAACGGTGGAGTATT+AGG | 0.287872 | 3_2:+70055913 | Msa0421170:CDS |
| ACCTGACCCCGCTTTGGAAT+CGG | 0.376203 | 3_2:-70055955 | None:intergenic |
| AGAAGGAGCAGCACATGATA+TGG | 0.377907 | 3_2:-70056034 | None:intergenic |
| TCCGATTCCAAAGCGGGGTC+AGG | 0.419316 | 3_2:+70055954 | Msa0421170:CDS |
| ATCACCACCTGACCCCGCTT+TGG | 0.431061 | 3_2:-70055961 | None:intergenic |
| AAGCGGGGTCAGGTGGTGAT+TGG | 0.451258 | 3_2:+70055964 | Msa0421170:CDS |
| TGACCGTCCGATTCCAAAGC+GGG | 0.457454 | 3_2:+70055948 | Msa0421170:CDS |
| GTGGTGATTGGAATTGTGTT+GGG | 0.460629 | 3_2:+70055976 | Msa0421170:CDS |
| TTACAAATTAAGCTAAAAGC+TGG | 0.468928 | 3_2:-70056065 | None:intergenic |
| GATTGGAATTGTGTTGGGAT+TGG | 0.472815 | 3_2:+70055981 | Msa0421170:CDS |
| GGTGGTGATTGGAATTGTGT+TGG | 0.485203 | 3_2:+70055975 | Msa0421170:CDS |
| AAAAGCTGGACTCTAGAAGA+AGG | 0.550483 | 3_2:-70056051 | None:intergenic |
| TGTGTTGGGATTGGCCAACT+CGG | 0.572810 | 3_2:+70055990 | Msa0421170:CDS |
| GAAGATGCAAACAACCGAGT+TGG | 0.598645 | 3_2:-70056004 | None:intergenic |
| TCAACGGTGGAGTATTAGGA+AGG | 0.608387 | 3_2:+70055917 | Msa0421170:CDS |
| GATTCCAAAGCGGGGTCAGG+TGG | 0.610196 | 3_2:+70055957 | Msa0421170:CDS |
| CAAAGCAAAGAGAAGATGGA+GGG | 0.621656 | 3_2:+70055859 | None:intergenic |
| ACTGCAATCATGAAAATCAA+CGG | 0.647400 | 3_2:+70055901 | Msa0421170:CDS |
| GACCCCGCTTTGGAATCGGA+CGG | 0.688444 | 3_2:-70055951 | None:intergenic |
| GCAATCATGAAAATCAACGG+TGG | 0.699924 | 3_2:+70055904 | Msa0421170:CDS |
| GACCGTCCGATTCCAAAGCG+GGG | 0.710779 | 3_2:+70055949 | Msa0421170:CDS |
| GTGACCGTCCGATTCCAAAG+CGG | 0.721311 | 3_2:+70055947 | Msa0421170:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTGCAATCATGAAAATCAA+CGG | + | chr3_2:70055901-70055920 | Msa0421170:CDS | 30.0% | |
| ! | AAAATCAACGGTGGAGTATT+AGG | + | chr3_2:70055913-70055932 | Msa0421170:CDS | 35.0% |
| GCAATCATGAAAATCAACGG+TGG | + | chr3_2:70055904-70055923 | Msa0421170:CDS | 40.0% | |
| GTGGTGATTGGAATTGTGTT+GGG | + | chr3_2:70055976-70055995 | Msa0421170:CDS | 40.0% | |
| GATTGGAATTGTGTTGGGAT+TGG | + | chr3_2:70055981-70056000 | Msa0421170:CDS | 40.0% | |
| ! | AAAAGCTGGACTCTAGAAGA+AGG | - | chr3_2:70056054-70056073 | None:intergenic | 40.0% |
| ! | TCAACGGTGGAGTATTAGGA+AGG | + | chr3_2:70055917-70055936 | Msa0421170:CDS | 45.0% |
| GGTGGTGATTGGAATTGTGT+TGG | + | chr3_2:70055975-70055994 | Msa0421170:CDS | 45.0% | |
| GAAGATGCAAACAACCGAGT+TGG | - | chr3_2:70056007-70056026 | None:intergenic | 45.0% | |
| AGAAGGAGCAGCACATGATA+TGG | - | chr3_2:70056037-70056056 | None:intergenic | 45.0% | |
| !! | TGTGTTGGGATTGGCCAACT+CGG | + | chr3_2:70055990-70056009 | Msa0421170:CDS | 50.0% |
| GTGACCGTCCGATTCCAAAG+CGG | + | chr3_2:70055947-70055966 | Msa0421170:CDS | 55.0% | |
| TGACCGTCCGATTCCAAAGC+GGG | + | chr3_2:70055948-70055967 | Msa0421170:CDS | 55.0% | |
| !! | ACCTGACCCCGCTTTGGAAT+CGG | - | chr3_2:70055958-70055977 | None:intergenic | 55.0% |
| GACCGTCCGATTCCAAAGCG+GGG | + | chr3_2:70055949-70055968 | Msa0421170:CDS | 60.0% | |
| !! | GACCCCGCTTTGGAATCGGA+CGG | - | chr3_2:70055954-70055973 | None:intergenic | 60.0% |
| TCCGATTCCAAAGCGGGGTC+AGG | + | chr3_2:70055954-70055973 | Msa0421170:CDS | 60.0% | |
| GATTCCAAAGCGGGGTCAGG+TGG | + | chr3_2:70055957-70055976 | Msa0421170:CDS | 60.0% | |
| ATCACCACCTGACCCCGCTT+TGG | - | chr3_2:70055964-70055983 | None:intergenic | 60.0% | |
| AAGCGGGGTCAGGTGGTGAT+TGG | + | chr3_2:70055964-70055983 | Msa0421170:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 70055874 | 70056080 | 70055874 | ID=Msa0421170;Name=Msa0421170 |
| chr3_2 | mRNA | 70055874 | 70056080 | 70055874 | ID=Msa0421170-mRNA-1;Parent=Msa0421170;Name=Msa0421170-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|68 |
| chr3_2 | exon | 70055874 | 70056080 | 70055874 | ID=Msa0421170-mRNA-1:exon:16726;Parent=Msa0421170-mRNA-1 |
| chr3_2 | CDS | 70055874 | 70056080 | 70055874 | ID=Msa0421170-mRNA-1:cds;Parent=Msa0421170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
