AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1439770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439770 MtrunA17_Chr8g0382671 96.774 186 6 0 1 186 1 186 7.95e-129 370
Msa1439770 MtrunA17_Chr7g0267911 73.575 193 35 3 1 186 1 184 2.78e-91 274
Msa1439770 MtrunA17_Chr3g0114811 28.313 166 109 4 26 186 14 174 2.23e-14 70.5
Msa1439770 MtrunA17_Chr3g0114831 39.535 86 51 1 102 186 452 537 4.11e-13 67.0
Msa1439770 MtrunA17_Chr3g0114831 35.238 105 65 2 83 186 19 121 3.90e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439770 AT2G22250.3 61.932 176 55 3 15 186 19 186 3.10e-64 205
Msa1439770 AT2G22250.2 61.932 176 55 3 15 186 19 186 3.10e-64 205
Msa1439770 AT2G22250.1 78.151 119 26 0 68 186 21 139 4.44e-63 201
Msa1439770 AT1G77670.1 30.128 156 100 4 32 186 20 167 5.64e-14 69.7

Find 67 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCACCCCGGTGCTCATTT+TGG 0.141862 tig0028866:+670 None:intergenic
GCAATTTGTCATAAGCTTAA+AGG 0.163237 tig0028866:-218 Msa1439770:intron
AATTCTAATGTTCCAGCATT+AGG 0.242169 tig0028866:+248 None:intergenic
TTGGCCATATCGGATAAATT+TGG 0.270402 tig0028866:+809 None:intergenic
GAGGGAGGGGTTTGGTGGAG+TGG 0.274032 tig0028866:+927 None:intergenic
AACGCAGCAAAATGGATTAT+TGG 0.290437 tig0028866:+878 None:intergenic
ATTCTAATGTTCCAGCATTA+GGG 0.292089 tig0028866:+249 None:intergenic
GTGGAGTGGAGGAGTGGGTG+AGG 0.316028 tig0028866:+941 None:intergenic
CTATCCAAATTTATCCGATA+TGG 0.328654 tig0028866:-813 Msa1439770:exon
GATCAGATTGTTGTTACTAA+TGG 0.334270 tig0028866:-76 Msa1439770:CDS
GGTTTGGTGGAGTGGAGGAG+TGG 0.334619 tig0028866:+935 None:intergenic
CTCAAAATCCGATTCTGAAT+TGG 0.336223 tig0028866:-618 Msa1439770:CDS
GGAGTGGAGGAGTGGGTGAG+GGG 0.341701 tig0028866:+943 None:intergenic
GGAGGGGTTTGGTGGAGTGG+AGG 0.347694 tig0028866:+930 None:intergenic
TGTTTCTTTCTTCATAATCC+TGG 0.349879 tig0028866:-854 Msa1439770:five_prime_UTR
AGAAACAAAACGCAGCAAAA+TGG 0.361488 tig0028866:+870 None:intergenic
CCGGCAGCCAAGCGAATAAC+AGG 0.376297 tig0028866:+497 None:intergenic
ATAGCGGGGAGAGATTTATC+AGG 0.386339 tig0028866:+746 None:intergenic
TGGAGTGGAGGAGTGGGTGA+GGG 0.391474 tig0028866:+942 None:intergenic
CAAAAGAGGGAGGGGTTTGG+TGG 0.410407 tig0028866:+922 None:intergenic
CCTGTTATTCGCTTGGCTGC+CGG 0.414314 tig0028866:-497 Msa1439770:CDS
GGGAAACGTGGAAAGGTTTG+AGG 0.414527 tig0028866:+691 None:intergenic
GAACAAAAGAGGGAGGGGTT+TGG 0.418447 tig0028866:+919 None:intergenic
TGGCGTTCCTGTTATTCGCT+TGG 0.434147 tig0028866:-504 Msa1439770:CDS
ATGCTGGAACATTAGAATTA+CGG 0.438442 tig0028866:-244 Msa1439770:CDS
ACCGCGGGTGTATCAAAATC+AGG 0.445970 tig0028866:+470 None:intergenic
GTTTGGTGGAGTGGAGGAGT+GGG 0.449452 tig0028866:+936 None:intergenic
ATCAACTTCCAATTCAGAAT+CGG 0.450108 tig0028866:+610 None:intergenic
GTGATTGCAGTTTGCTCACC+GGG 0.475286 tig0028866:-28 Msa1439770:CDS
AGTGGGTGAGGGGAGAGAGT+TGG 0.477560 tig0028866:+953 None:intergenic
TAAGAATGTGTTGGCCATAT+CGG 0.480353 tig0028866:+799 None:intergenic
GTAATAGCGACAGTTCTTGA+AGG 0.496768 tig0028866:+554 None:intergenic
GCCACTGCTCTTGTTCAAGC+TGG 0.498280 tig0028866:-524 Msa1439770:CDS
CGTTTCCCAAAATGAGCACC+GGG 0.499708 tig0028866:-676 Msa1439770:CDS
AAGAAGAACAAAAGAGGGAG+GGG 0.500944 tig0028866:+914 None:intergenic
GAGAGATCTGTGGCGAGAAA+CGG 0.503955 tig0028866:+721 None:intergenic
TGGCGAGAAACGGAAATAGC+GGG 0.522526 tig0028866:+731 None:intergenic
CAAAATGGATTATTGGTAAG+TGG 0.523259 tig0028866:+885 None:intergenic
AGTGATTGCAGTTTGCTCAC+CGG 0.523981 tig0028866:-29 Msa1439770:CDS
ACGTTTCCCAAAATGAGCAC+CGG 0.529984 tig0028866:-677 Msa1439770:CDS
GTGAGAAGAAGAACAAAAGA+GGG 0.535755 tig0028866:+909 None:intergenic
ACAAGGTATACCCCTAATGC+TGG 0.536420 tig0028866:-260 Msa1439770:CDS
TGTGAGAAGAAGAACAAAAG+AGG 0.539593 tig0028866:+908 None:intergenic
AGAAGAAGAACAAAAGAGGG+AGG 0.554543 tig0028866:+912 None:intergenic
GAAGAAGAACAAAAGAGGGA+GGG 0.563723 tig0028866:+913 None:intergenic
TCTTCTGTTCTGCTCCACCC+CGG 0.567889 tig0028866:+658 None:intergenic
TGTATCAAAATCAGGTTCAC+CGG 0.576041 tig0028866:+478 None:intergenic
GTTGTTGTGTAAGAATGTGT+TGG 0.584819 tig0028866:+790 None:intergenic
AGCTAAGCAGAGTATTACTC+AGG 0.591051 tig0028866:-53 Msa1439770:CDS
AATACCTCGGCAATAACCGC+GGG 0.593217 tig0028866:+455 None:intergenic
AGTTTGCTCACCGGGAGATG+AGG 0.598945 tig0028866:-20 Msa1439770:CDS
TATGTAACTGAATCTAAACC+AGG 0.621769 tig0028866:+836 None:intergenic
AAATACCTCGGCAATAACCG+CGG 0.623880 tig0028866:+454 None:intergenic
GGGATTAATGCAATTCGTGA+AGG 0.627742 tig0028866:-287 Msa1439770:CDS
TTCTAATGTTCCAGCATTAG+GGG 0.630270 tig0028866:+250 None:intergenic
TCCCAAAATGAGCACCGGGG+TGG 0.635586 tig0028866:-672 Msa1439770:CDS
GTTGGTGAAATTGTATAACT+CGG 0.640826 tig0028866:+971 None:intergenic
TACACCCGCGGTTATTGCCG+AGG 0.640881 tig0028866:-459 Msa1439770:intron
TAGCGGGGAGAGATTTATCA+GGG 0.647814 tig0028866:+747 None:intergenic
GTGGCGAGAAACGGAAATAG+CGG 0.650340 tig0028866:+730 None:intergenic
AGGAGAAAGAGAGAGATCTG+TGG 0.651546 tig0028866:+711 None:intergenic
GCCAGCTTGAACAAGAGCAG+TGG 0.668141 tig0028866:+523 None:intergenic
GGTTTGACAGCATTAACACG+TGG 0.682883 tig0028866:+575 None:intergenic
CAATTCGTGAAGGATACACA+AGG 0.683943 tig0028866:-277 Msa1439770:CDS
GGCGAGAAACGGAAATAGCG+GGG 0.690573 tig0028866:+732 None:intergenic
AGGGAAAGAGATTGTACACG+TGG 0.705011 tig0028866:+766 None:intergenic
GTTTCCCAAAATGAGCACCG+GGG 0.831303 tig0028866:-675 Msa1439770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCAAATTGAAAATACCT+CGG + tig0028866:548-567 None:intergenic 20.0%
!! AATAAACTAGAAACAACTTA+TGG + tig0028866:635-654 None:intergenic 20.0%
!!! TTAGTTTTATTACTTGTGTA+TGG - tig0028866:666-685 Msa1439770:CDS 20.0%
!!! CTGTATTATATGGTTTTATT+TGG - tig0028866:806-825 Msa1439770:exon 20.0%
!! AATTCAAATTGAAAATACCT+CGG + tig0028866:548-567 None:intergenic 20.0%
!! AATAAACTAGAAACAACTTA+TGG + tig0028866:635-654 None:intergenic 20.0%
!!! TTAGTTTTATTACTTGTGTA+TGG - tig0028866:666-685 Msa1439770:CDS 20.0%
!!! CTGTATTATATGGTTTTATT+TGG - tig0028866:806-825 Msa1439770:exon 20.0%
! TTTGATTGTGCTGTATTATA+TGG - tig0028866:796-815 Msa1439770:CDS 25.0%
! TTTGATTGTGCTGTATTATA+TGG - tig0028866:796-815 Msa1439770:CDS 25.0%
! GTTGGTGAAATTGTATAACT+CGG + tig0028866:19-38 None:intergenic 30.0%
!! CAAAATGGATTATTGGTAAG+TGG + tig0028866:105-124 None:intergenic 30.0%
TGTTTCTTTCTTCATAATCC+TGG - tig0028866:133-152 Msa1439770:intron 30.0%
TATGTAACTGAATCTAAACC+AGG + tig0028866:154-173 None:intergenic 30.0%
CTATCCAAATTTATCCGATA+TGG - tig0028866:174-193 Msa1439770:intron 30.0%
ATCAACTTCCAATTCAGAAT+CGG + tig0028866:380-399 None:intergenic 30.0%
ACTTATGGACATGTTAAAAG+TGG + tig0028866:620-639 None:intergenic 30.0%
!! GTTTCTAGTTTATTCGAACA+TGG - tig0028866:641-660 Msa1439770:CDS 30.0%
! TTACTTGTGTATGGTAATGT+AGG - tig0028866:675-694 Msa1439770:CDS 30.0%
ATTCTAATGTTCCAGCATTA+GGG + tig0028866:741-760 None:intergenic 30.0%
AATTCTAATGTTCCAGCATT+AGG + tig0028866:742-761 None:intergenic 30.0%
ATGCTGGAACATTAGAATTA+CGG - tig0028866:743-762 Msa1439770:CDS 30.0%
GCAATTTGTCATAAGCTTAA+AGG - tig0028866:769-788 Msa1439770:CDS 30.0%
TGGATTAATTACATGAGATG+TGG - tig0028866:826-845 Msa1439770:five_prime_UTR 30.0%
! TTTTGACAATGTGTCGTAAT+TGG - tig0028866:850-869 Msa1439770:five_prime_UTR 30.0%
TTAGTAACAACAATCTGATC+AGG + tig0028866:911-930 None:intergenic 30.0%
! GATCAGATTGTTGTTACTAA+TGG - tig0028866:911-930 Msa1439770:five_prime_UTR 30.0%
! GTTGGTGAAATTGTATAACT+CGG + tig0028866:19-38 None:intergenic 30.0%
!! CAAAATGGATTATTGGTAAG+TGG + tig0028866:105-124 None:intergenic 30.0%
TGTTTCTTTCTTCATAATCC+TGG - tig0028866:133-152 Msa1439770:intron 30.0%
TATGTAACTGAATCTAAACC+AGG + tig0028866:154-173 None:intergenic 30.0%
CTATCCAAATTTATCCGATA+TGG - tig0028866:174-193 Msa1439770:intron 30.0%
ATCAACTTCCAATTCAGAAT+CGG + tig0028866:380-399 None:intergenic 30.0%
ACTTATGGACATGTTAAAAG+TGG + tig0028866:620-639 None:intergenic 30.0%
!! GTTTCTAGTTTATTCGAACA+TGG - tig0028866:641-660 Msa1439770:CDS 30.0%
! TTACTTGTGTATGGTAATGT+AGG - tig0028866:675-694 Msa1439770:CDS 30.0%
ATTCTAATGTTCCAGCATTA+GGG + tig0028866:741-760 None:intergenic 30.0%
AATTCTAATGTTCCAGCATT+AGG + tig0028866:742-761 None:intergenic 30.0%
ATGCTGGAACATTAGAATTA+CGG - tig0028866:743-762 Msa1439770:CDS 30.0%
GCAATTTGTCATAAGCTTAA+AGG - tig0028866:769-788 Msa1439770:CDS 30.0%
TGGATTAATTACATGAGATG+TGG - tig0028866:826-845 Msa1439770:five_prime_UTR 30.0%
! TTTTGACAATGTGTCGTAAT+TGG - tig0028866:850-869 Msa1439770:five_prime_UTR 30.0%
TTAGTAACAACAATCTGATC+AGG + tig0028866:911-930 None:intergenic 30.0%
! GATCAGATTGTTGTTACTAA+TGG - tig0028866:911-930 Msa1439770:five_prime_UTR 30.0%
GTGAGAAGAAGAACAAAAGA+GGG + tig0028866:81-100 None:intergenic 35.0%
TGTGAGAAGAAGAACAAAAG+AGG + tig0028866:82-101 None:intergenic 35.0%
!! AACGCAGCAAAATGGATTAT+TGG + tig0028866:112-131 None:intergenic 35.0%
AGAAACAAAACGCAGCAAAA+TGG + tig0028866:120-139 None:intergenic 35.0%
TTGGCCATATCGGATAAATT+TGG + tig0028866:181-200 None:intergenic 35.0%
! TAAGAATGTGTTGGCCATAT+CGG + tig0028866:191-210 None:intergenic 35.0%
GTTGTTGTGTAAGAATGTGT+TGG + tig0028866:200-219 None:intergenic 35.0%
CTCAAAATCCGATTCTGAAT+TGG - tig0028866:369-388 Msa1439770:intron 35.0%
TGTATCAAAATCAGGTTCAC+CGG + tig0028866:512-531 None:intergenic 35.0%
TTCTAATGTTCCAGCATTAG+GGG + tig0028866:740-759 None:intergenic 35.0%
!! GTAATTGGTTGATTTTGCAG+AGG - tig0028866:865-884 Msa1439770:five_prime_UTR 35.0%
GTGAGAAGAAGAACAAAAGA+GGG + tig0028866:81-100 None:intergenic 35.0%
TGTGAGAAGAAGAACAAAAG+AGG + tig0028866:82-101 None:intergenic 35.0%
!! AACGCAGCAAAATGGATTAT+TGG + tig0028866:112-131 None:intergenic 35.0%
AGAAACAAAACGCAGCAAAA+TGG + tig0028866:120-139 None:intergenic 35.0%
TTGGCCATATCGGATAAATT+TGG + tig0028866:181-200 None:intergenic 35.0%
! TAAGAATGTGTTGGCCATAT+CGG + tig0028866:191-210 None:intergenic 35.0%
GTTGTTGTGTAAGAATGTGT+TGG + tig0028866:200-219 None:intergenic 35.0%
CTCAAAATCCGATTCTGAAT+TGG - tig0028866:369-388 Msa1439770:intron 35.0%
TGTATCAAAATCAGGTTCAC+CGG + tig0028866:512-531 None:intergenic 35.0%
TTCTAATGTTCCAGCATTAG+GGG + tig0028866:740-759 None:intergenic 35.0%
!! GTAATTGGTTGATTTTGCAG+AGG - tig0028866:865-884 Msa1439770:five_prime_UTR 35.0%
AAGAAGAACAAAAGAGGGAG+GGG + tig0028866:76-95 None:intergenic 40.0%
GAAGAAGAACAAAAGAGGGA+GGG + tig0028866:77-96 None:intergenic 40.0%
AGAAGAAGAACAAAAGAGGG+AGG + tig0028866:78-97 None:intergenic 40.0%
!! TCATTTTGGGAAACGTGGAA+AGG + tig0028866:306-325 None:intergenic 40.0%
GTAATAGCGACAGTTCTTGA+AGG + tig0028866:436-455 None:intergenic 40.0%
! TTGTGTATGGTAATGTAGGC+TGG - tig0028866:679-698 Msa1439770:CDS 40.0%
! TGTGTATGGTAATGTAGGCT+GGG - tig0028866:680-699 Msa1439770:CDS 40.0%
GGGATTAATGCAATTCGTGA+AGG - tig0028866:700-719 Msa1439770:CDS 40.0%
CAATTCGTGAAGGATACACA+AGG - tig0028866:710-729 Msa1439770:CDS 40.0%
!! GTTGATTTTGCAGAGGAGAA+TGG - tig0028866:872-891 Msa1439770:five_prime_UTR 40.0%
! AGCTAAGCAGAGTATTACTC+AGG - tig0028866:934-953 Msa1439770:five_prime_UTR 40.0%
AAGAAGAACAAAAGAGGGAG+GGG + tig0028866:76-95 None:intergenic 40.0%
GAAGAAGAACAAAAGAGGGA+GGG + tig0028866:77-96 None:intergenic 40.0%
AGAAGAAGAACAAAAGAGGG+AGG + tig0028866:78-97 None:intergenic 40.0%
!! TCATTTTGGGAAACGTGGAA+AGG + tig0028866:306-325 None:intergenic 40.0%
GTAATAGCGACAGTTCTTGA+AGG + tig0028866:436-455 None:intergenic 40.0%
! TTGTGTATGGTAATGTAGGC+TGG - tig0028866:679-698 Msa1439770:CDS 40.0%
! TGTGTATGGTAATGTAGGCT+GGG - tig0028866:680-699 Msa1439770:CDS 40.0%
GGGATTAATGCAATTCGTGA+AGG - tig0028866:700-719 Msa1439770:CDS 40.0%
CAATTCGTGAAGGATACACA+AGG - tig0028866:710-729 Msa1439770:CDS 40.0%
!! GTTGATTTTGCAGAGGAGAA+TGG - tig0028866:872-891 Msa1439770:five_prime_UTR 40.0%
! AGCTAAGCAGAGTATTACTC+AGG - tig0028866:934-953 Msa1439770:five_prime_UTR 40.0%
AGGGAAAGAGATTGTACACG+TGG + tig0028866:224-243 None:intergenic 45.0%
TAGCGGGGAGAGATTTATCA+GGG + tig0028866:243-262 None:intergenic 45.0%
! ATAGCGGGGAGAGATTTATC+AGG + tig0028866:244-263 None:intergenic 45.0%
AGGAGAAAGAGAGAGATCTG+TGG + tig0028866:279-298 None:intergenic 45.0%
ACGTTTCCCAAAATGAGCAC+CGG - tig0028866:310-329 Msa1439770:intron 45.0%
GGTTTGACAGCATTAACACG+TGG + tig0028866:415-434 None:intergenic 45.0%
!! ACCTGATTTTGATACACCCG+CGG - tig0028866:516-535 Msa1439770:CDS 45.0%
AAATACCTCGGCAATAACCG+CGG + tig0028866:536-555 None:intergenic 45.0%
ACAAGGTATACCCCTAATGC+TGG - tig0028866:727-746 Msa1439770:CDS 45.0%
! AGTGATTGCAGTTTGCTCAC+CGG - tig0028866:958-977 Msa1439770:five_prime_UTR 45.0%
AGGGAAAGAGATTGTACACG+TGG + tig0028866:224-243 None:intergenic 45.0%
TAGCGGGGAGAGATTTATCA+GGG + tig0028866:243-262 None:intergenic 45.0%
! ATAGCGGGGAGAGATTTATC+AGG + tig0028866:244-263 None:intergenic 45.0%
AGGAGAAAGAGAGAGATCTG+TGG + tig0028866:279-298 None:intergenic 45.0%
ACGTTTCCCAAAATGAGCAC+CGG - tig0028866:310-329 Msa1439770:intron 45.0%
GGTTTGACAGCATTAACACG+TGG + tig0028866:415-434 None:intergenic 45.0%
!! ACCTGATTTTGATACACCCG+CGG - tig0028866:516-535 Msa1439770:CDS 45.0%
AAATACCTCGGCAATAACCG+CGG + tig0028866:536-555 None:intergenic 45.0%
ACAAGGTATACCCCTAATGC+TGG - tig0028866:727-746 Msa1439770:CDS 45.0%
! AGTGATTGCAGTTTGCTCAC+CGG - tig0028866:958-977 Msa1439770:five_prime_UTR 45.0%
GAACAAAAGAGGGAGGGGTT+TGG + tig0028866:71-90 None:intergenic 50.0%
TGGCGAGAAACGGAAATAGC+GGG + tig0028866:259-278 None:intergenic 50.0%
GTGGCGAGAAACGGAAATAG+CGG + tig0028866:260-279 None:intergenic 50.0%
GAGAGATCTGTGGCGAGAAA+CGG + tig0028866:269-288 None:intergenic 50.0%
GGGAAACGTGGAAAGGTTTG+AGG + tig0028866:299-318 None:intergenic 50.0%
!! GGTGCTCATTTTGGGAAACG+TGG + tig0028866:311-330 None:intergenic 50.0%
CGTTTCCCAAAATGAGCACC+GGG - tig0028866:311-330 Msa1439770:intron 50.0%
! GTTTCCCAAAATGAGCACCG+GGG - tig0028866:312-331 Msa1439770:intron 50.0%
TGGCGTTCCTGTTATTCGCT+TGG - tig0028866:483-502 Msa1439770:CDS 50.0%
ACCGCGGGTGTATCAAAATC+AGG + tig0028866:520-539 None:intergenic 50.0%
AATACCTCGGCAATAACCGC+GGG + tig0028866:535-554 None:intergenic 50.0%
! GTGATTGCAGTTTGCTCACC+GGG - tig0028866:959-978 Msa1439770:five_prime_UTR 50.0%
GAACAAAAGAGGGAGGGGTT+TGG + tig0028866:71-90 None:intergenic 50.0%
TGGCGAGAAACGGAAATAGC+GGG + tig0028866:259-278 None:intergenic 50.0%
GTGGCGAGAAACGGAAATAG+CGG + tig0028866:260-279 None:intergenic 50.0%
GAGAGATCTGTGGCGAGAAA+CGG + tig0028866:269-288 None:intergenic 50.0%
GGGAAACGTGGAAAGGTTTG+AGG + tig0028866:299-318 None:intergenic 50.0%
!! GGTGCTCATTTTGGGAAACG+TGG + tig0028866:311-330 None:intergenic 50.0%
CGTTTCCCAAAATGAGCACC+GGG - tig0028866:311-330 Msa1439770:intron 50.0%
! GTTTCCCAAAATGAGCACCG+GGG - tig0028866:312-331 Msa1439770:intron 50.0%
TGGCGTTCCTGTTATTCGCT+TGG - tig0028866:483-502 Msa1439770:CDS 50.0%
ACCGCGGGTGTATCAAAATC+AGG + tig0028866:520-539 None:intergenic 50.0%
AATACCTCGGCAATAACCGC+GGG + tig0028866:535-554 None:intergenic 50.0%
! GTGATTGCAGTTTGCTCACC+GGG - tig0028866:959-978 Msa1439770:five_prime_UTR 50.0%
! GTTTGGTGGAGTGGAGGAGT+GGG + tig0028866:54-73 None:intergenic 55.0%
CAAAAGAGGGAGGGGTTTGG+TGG + tig0028866:68-87 None:intergenic 55.0%
GGCGAGAAACGGAAATAGCG+GGG + tig0028866:258-277 None:intergenic 55.0%
! TCCACCCCGGTGCTCATTTT+GGG + tig0028866:319-338 None:intergenic 55.0%
TCTTCTGTTCTGCTCCACCC+CGG + tig0028866:332-351 None:intergenic 55.0%
GCCACTGCTCTTGTTCAAGC+TGG - tig0028866:463-482 Msa1439770:CDS 55.0%
GCCAGCTTGAACAAGAGCAG+TGG + tig0028866:467-486 None:intergenic 55.0%
CCTGTTATTCGCTTGGCTGC+CGG - tig0028866:490-509 Msa1439770:CDS 55.0%
! AGTTTGCTCACCGGGAGATG+AGG - tig0028866:967-986 Msa1439770:five_prime_UTR 55.0%
! GTTTGGTGGAGTGGAGGAGT+GGG + tig0028866:54-73 None:intergenic 55.0%
CAAAAGAGGGAGGGGTTTGG+TGG + tig0028866:68-87 None:intergenic 55.0%
GGCGAGAAACGGAAATAGCG+GGG + tig0028866:258-277 None:intergenic 55.0%
! TCCACCCCGGTGCTCATTTT+GGG + tig0028866:319-338 None:intergenic 55.0%
TCTTCTGTTCTGCTCCACCC+CGG + tig0028866:332-351 None:intergenic 55.0%
GCCACTGCTCTTGTTCAAGC+TGG - tig0028866:463-482 Msa1439770:CDS 55.0%
GCCAGCTTGAACAAGAGCAG+TGG + tig0028866:467-486 None:intergenic 55.0%
CCTGTTATTCGCTTGGCTGC+CGG - tig0028866:490-509 Msa1439770:CDS 55.0%
! AGTTTGCTCACCGGGAGATG+AGG - tig0028866:967-986 Msa1439770:five_prime_UTR 55.0%
AGTGGGTGAGGGGAGAGAGT+TGG + tig0028866:37-56 None:intergenic 60.0%
TGGAGTGGAGGAGTGGGTGA+GGG + tig0028866:48-67 None:intergenic 60.0%
!! GGTTTGGTGGAGTGGAGGAG+TGG + tig0028866:55-74 None:intergenic 60.0%
!! TCCCAAAATGAGCACCGGGG+TGG - tig0028866:315-334 Msa1439770:intron 60.0%
! CTCCACCCCGGTGCTCATTT+TGG + tig0028866:320-339 None:intergenic 60.0%
CCGGCAGCCAAGCGAATAAC+AGG + tig0028866:493-512 None:intergenic 60.0%
TACACCCGCGGTTATTGCCG+AGG - tig0028866:528-547 Msa1439770:CDS 60.0%
AGTGGGTGAGGGGAGAGAGT+TGG + tig0028866:37-56 None:intergenic 60.0%
TGGAGTGGAGGAGTGGGTGA+GGG + tig0028866:48-67 None:intergenic 60.0%
!! GGTTTGGTGGAGTGGAGGAG+TGG + tig0028866:55-74 None:intergenic 60.0%
!! TCCCAAAATGAGCACCGGGG+TGG - tig0028866:315-334 Msa1439770:intron 60.0%
! CTCCACCCCGGTGCTCATTT+TGG + tig0028866:320-339 None:intergenic 60.0%
CCGGCAGCCAAGCGAATAAC+AGG + tig0028866:493-512 None:intergenic 60.0%
TACACCCGCGGTTATTGCCG+AGG - tig0028866:528-547 Msa1439770:CDS 60.0%
GGAGTGGAGGAGTGGGTGAG+GGG + tig0028866:47-66 None:intergenic 65.0%
GTGGAGTGGAGGAGTGGGTG+AGG + tig0028866:49-68 None:intergenic 65.0%
!! GGAGGGGTTTGGTGGAGTGG+AGG + tig0028866:60-79 None:intergenic 65.0%
!! GAGGGAGGGGTTTGGTGGAG+TGG + tig0028866:63-82 None:intergenic 65.0%
GGAGTGGAGGAGTGGGTGAG+GGG + tig0028866:47-66 None:intergenic 65.0%
GTGGAGTGGAGGAGTGGGTG+AGG + tig0028866:49-68 None:intergenic 65.0%
!! GGAGGGGTTTGGTGGAGTGG+AGG + tig0028866:60-79 None:intergenic 65.0%
!! GAGGGAGGGGTTTGGTGGAG+TGG + tig0028866:63-82 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0028866 gene 12 997 12 ID=Msa1439770;Name=Msa1439770
tig0028866 mRNA 12 997 12 ID=Msa1439770-mRNA-1;Parent=Msa1439770;Name=Msa1439770-mRNA-1;_AED=0.01;_eAED=0.01;_QI=181|1|0.66|1|1|1|3|0|188
tig0028866 exon 12 124 12 ID=Msa1439770-mRNA-1:exon:11956;Parent=Msa1439770-mRNA-1
tig0028866 exon 219 312 219 ID=Msa1439770-mRNA-1:exon:11955;Parent=Msa1439770-mRNA-1
tig0028866 exon 460 997 460 ID=Msa1439770-mRNA-1:exon:11954;Parent=Msa1439770-mRNA-1
tig0028866 five_prime_UTR 817 997 817 ID=Msa1439770-mRNA-1:five_prime_utr;Parent=Msa1439770-mRNA-1
tig0028866 CDS 460 816 460 ID=Msa1439770-mRNA-1:cds;Parent=Msa1439770-mRNA-1
tig0028866 CDS 219 312 219 ID=Msa1439770-mRNA-1:cds;Parent=Msa1439770-mRNA-1
tig0028866 CDS 12 124 12 ID=Msa1439770-mRNA-1:cds;Parent=Msa1439770-mRNA-1
Gene Sequence

>Msa1439770

ATGGCCAACACATTCTTACACAACAACACCACGTGTACAATCTCTTTCCCTGATAAATCTCTCCCCGCTATTTCCGTTTCTCGCCACAGATCTCTCTCTTTCTCCTCAAACCTTTCCACGTTTCCCAAAATGAGCACCGGGGTGGAGCAGAACAGAAGAGAAACAATAATTGTCAACTCAAAATCCGATTCTGAATTGGAAGTTGATATTTCATTGAGTCCACGTGTTAATGCTGTCAAACCTTCAAGAACTGTCGCTATTACTGATCAAGCCACTGCTCTTGTTCAAGCTGGCGTTCCTGTTATTCGCTTGGCTGCCGGTGAACCTGATTTTGATACACCCGCGGTTATTGCCGAGGCTGGGATTAATGCAATTCGTGAAGGATACACAAGGTATACCCCTAATGCTGGAACATTAGAATTACGGCAAGCAATTTGTCATAAGCTTAAAGAGGAGAATGGACTTGATTATACTCCTGATCAGATTGTTGTTACTAATGGAGCTAAGCAGAGTATTACTCAGGCAGTGATTGCAGTTTGCTCACCGGGAGATGAGGTGAGTAAT
Protein sequence

>Msa1439770

MANTFLHNNTTCTISFPDKSLPAISVSRHRSLSFSSNLSTFPKMSTGVEQNRRETIIVNSKSDSELEVDISLSPRVNAVKPSRTVAITDQATALVQAGVPVIRLAAGEPDFDTPAVIAEAGINAIREGYTRYTPNAGTLELRQAICHKLKEENGLDYTPDQIVVTNGAKQSITQAVIAVCSPGDEVSN