AlfalfaGEDB | Msa1444710

M. sativa cultivar zhongmu-4 / Msa1444710

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444710	sp\|Q9SZL8\|FRS5_ARATH	41.121	107	58	1	174	275	429	535	3.03e-20	97.4
Msa1444710	sp\|Q9SZL8\|FRS5_ARATH	36.486	148	72	5	36	166	59	201	1.93e-15	82.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444710	A0A6A5MW98	54.206	321	101	6	172	453	101	414	6.45e-105	328

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TFs/TRs:

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Gene ID	Type	Classification
Msa1444710	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1208310	Msa1444710	0.916577	1.506489e-85	-8.615850e-47
Msa1444150	Msa1444710	0.993158	7.723570e-198	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444710	MtrunA17_Chr5g0448761	42.991	107	56	1	174	275	420	526	1.82e-21	97.8
Msa1444710	MtrunA17_Chr5g0448761	36.765	136	67	5	45	166	59	189	3.28e-15	78.2
Msa1444710	MtrunA17_Chr1g0202341	38.095	126	66	3	165	280	397	520	4.33e-19	90.1
Msa1444710	MtrunA17_Chr1g0202341	30.058	173	101	6	10	170	17	181	2.17e-12	69.3
Msa1444710	MtrunA17_Chr1g0196541	37.398	123	64	2	146	255	309	431	1.92e-18	88.2
Msa1444710	MtrunA17_Chr5g0441581	37.838	111	64	1	150	255	349	459	2.70e-17	84.7
Msa1444710	MtrunA17_Chr2g0296781	42.529	87	45	1	174	255	345	431	1.04e-16	82.8
Msa1444710	MtrunA17_Chr5g0448511	39.252	107	60	1	174	275	192	298	1.21e-16	82.4
Msa1444710	MtrunA17_Chr6g0477261	42.529	87	45	1	174	255	234	320	1.22e-16	82.8
Msa1444710	MtrunA17_Chr3g0080351	34.545	110	67	1	171	275	416	525	2.41e-14	75.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444710	AT4G38180.1	41.121	107	58	1	174	275	429	535	3.08e-21	97.4
Msa1444710	AT4G38180.1	36.486	148	72	5	36	166	59	201	1.96e-16	82.4
Msa1444710	AT4G15090.1	36.111	108	64	1	171	273	402	509	1.43e-17	86.3
Msa1444710	AT4G15090.5	36.111	108	64	1	171	273	402	509	1.43e-17	86.3
Msa1444710	AT4G15090.3	36.111	108	64	1	171	273	402	509	1.43e-17	86.3
Msa1444710	AT4G15090.2	36.111	108	64	1	171	273	402	509	1.43e-17	86.3
Msa1444710	AT4G15090.4	36.111	108	64	1	171	273	402	509	1.43e-17	86.3
Msa1444710	AT4G38170.1	33.645	107	66	1	174	275	194	300	6.09e-16	80.5
Msa1444710	AT3G22170.2	32.174	115	69	2	170	275	406	520	5.24e-14	75.1
Msa1444710	AT3G22170.1	32.174	115	69	2	170	275	406	520	5.24e-14	75.1

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Find 56 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGCTTCTATTATAAAC+TGG	0.392497	tig0030801:-31390	None:intergenic
AAAGAGAGGATTATGAAAGT+AGG	0.500092	tig0030801:+31550	Msa1444710:CDS
AAGTGTTCTACTTCTCGAAC+TGG	0.426697	tig0030801:+30458	Msa1444710:CDS
AATTCATTATATCTGATTTG+AGG	0.445916	tig0030801:+32063	Msa1444710:CDS
ACAAAAGGGAGAAAGAAGAA+TGG	0.552574	tig0030801:+32242	Msa1444710:CDS
ACAACAATGAATGGCTTCAA+GGG	0.573168	tig0030801:+31340	Msa1444710:CDS
ACAGGTGCATTGGTCAATTA+TGG	0.275145	tig0030801:+30130	Msa1444710:intron
ACGGACTGGACAACAATGAA+TGG	0.575271	tig0030801:+31331	Msa1444710:CDS
AGAAAAGCATGTCATGAGTG+TGG	0.709074	tig0030801:+32338	Msa1444710:CDS
AGCAAAGGTATTAGTTGATT+TGG	0.254141	tig0030801:+32016	Msa1444710:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGATATGGGAAATGTTAAC+CGG	+	tig0030801:27902-27921	Msa1444710:intron	30.0%
GCTAATGGCCTAATAATATA+GGG	-	tig0030801:28394-28413	None:intergenic	30.0%
ATCCTTAAACAACATCATCA+AGG	+	tig0030801:28709-28728	Msa1444710:intron	30.0%
TTGCTTATAATTGACAACGA+AGG	+	tig0030801:29170-29189	Msa1444710:intron	30.0%
ACCTCATTAAAAGTTTAGAG+TGG	+	tig0030801:29400-29419	Msa1444710:intron	30.0%
TAAGTGTTTAGACTTACTCT+TGG	-	tig0030801:29439-29458	None:intergenic	30.0%
AAGATAAATGTGTGTAGATG+TGG	-	tig0030801:29548-29567	None:intergenic	30.0%
AAGAAAGGAATCAGAAAAAG+AGG	-	tig0030801:29779-29798	None:intergenic	30.0%
AAATCGATGCCTTTCTAATT+TGG	+	tig0030801:29845-29864	Msa1444710:intron	30.0%
TATAGTGCTATACTTATCGT+GGG	+	tig0030801:29911-29930	Msa1444710:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0030801	gene	27772	32412	27772	ID=Msa1444710;Name=Msa1444710
tig0030801	mRNA	27772	32412	27772	ID=Msa1444710-mRNA-1;Parent=Msa1444710;Name=Msa1444710-mRNA-1;_AED=0.08;_eAED=0.13;_QI=0\|0\|0\|0.75\|0.28\|0.25\|8\|0\|462
tig0030801	exon	27772	27777	27772	ID=Msa1444710-mRNA-1:exon:14602;Parent=Msa1444710-mRNA-1
tig0030801	exon	28571	28576	28571	ID=Msa1444710-mRNA-1:exon:14603;Parent=Msa1444710-mRNA-1
tig0030801	exon	30134	30283	30134	ID=Msa1444710-mRNA-1:exon:14604;Parent=Msa1444710-mRNA-1
tig0030801	exon	30293	30509	30293	ID=Msa1444710-mRNA-1:exon:14605;Parent=Msa1444710-mRNA-1
tig0030801	exon	30522	30656	30522	ID=Msa1444710-mRNA-1:exon:14606;Parent=Msa1444710-mRNA-1
tig0030801	exon	31304	31361	31304	ID=Msa1444710-mRNA-1:exon:14607;Parent=Msa1444710-mRNA-1
tig0030801	exon	31377	31622	31377	ID=Msa1444710-mRNA-1:exon:14608;Parent=Msa1444710-mRNA-1
tig0030801	exon	31842	32412	31842	ID=Msa1444710-mRNA-1:exon:14609;Parent=Msa1444710-mRNA-1
tig0030801	CDS	27772	27777	27772	ID=Msa1444710-mRNA-1:cds;Parent=Msa1444710-mRNA-1
tig0030801	CDS	28571	28576	28571	ID=Msa1444710-mRNA-1:cds;Parent=Msa1444710-mRNA-1
tig0030801	CDS	30134	30283	30134	ID=Msa1444710-mRNA-1:cds;Parent=Msa1444710-mRNA-1
tig0030801	CDS	30293	30509	30293	ID=Msa1444710-mRNA-1:cds;Parent=Msa1444710-mRNA-1
tig0030801	CDS	30522	30656	30522	ID=Msa1444710-mRNA-1:cds;Parent=Msa1444710-mRNA-1
tig0030801	CDS	31304	31361	31304	ID=Msa1444710-mRNA-1:cds;Parent=Msa1444710-mRNA-1
tig0030801	CDS	31377	31622	31377	ID=Msa1444710-mRNA-1:cds;Parent=Msa1444710-mRNA-1
tig0030801	CDS	31842	32412	31842	ID=Msa1444710-mRNA-1:cds;Parent=Msa1444710-mRNA-1

Gene Sequence

>Msa1444710

ATGGATAGCACTGTGCATTGGTCAATTATGGATGGTAATGTTTTTGAAGAAGCCACACAGTCAACAAATGTGGAAATATCTGAAGAAGATATTTTAGCTGCTGCTCCTAATGTTGGTGAGGAATTAAATAGTGAACCTTGTAAGGGGATGGAATTCAAATCTGTTAAAGAATTTTACAATTCATTTGCAAAAAGGATGGGTTTTGGAGTACGTGTTCGCTCAACTAAACCAAAGAGAGCTGTCTTTGTATGTTGTAATGAAGGTCATCATATAGTGAAAAGTTCAATAAACAAAGAAATCCAAGATGACACAAATCAAACTAAAAGAAAGTGTTCTACTTCTCGAACTGGTTGTCTAGCCACACTTGTTGTTAAAAGAGGAAATTGGATTATATGTTCATTTAAGAATGATCATAACCATCCCATGGTTAGCCCTAAAAGTGTGTCTTATATGCGGTGTCATAAAAGCATGAATGGTGCTGCAAAAAGTCTTGTTGAACATTTTAAGGAAGATGAAGACTGGAGCCGATTAATGATTGAGTACGGACTGGACAACAATGAATGGCTTCAAGGAGAATCTTGGATACCAGTTTATAATAGAAGCTGTTTTTTTGCTGGGATGAATACAAGTCAAAGAAGTGAGAGTATCAATGCGTTTTTTGATTCTTTTGTGAATGCAACTACAACATTGCAAGAATTTGTGTTGAAATTTGAAAAGGCGGTTGATAGCCGCTTAGAAGTAGAAAAAAGAGAGGATTATGAAAGTAGGCATAAAAAACGCATATTGTCTACAGGGTCAAAACTTGAGGAGCACGCTGCGCATATACTCGTAATTTTTCAAGCAAAAGGTATCACTCAAATTCCTGACCATTTTATTTTGCAGCGTTGCACTAAAAATGCTAACAAAAGCATTGAAGTTTGTGATACGACGAGTAATTTTGAAGGTCAAAATAGCACGCCAAAGATATTAAGAAGGATGCATGCTCAACAAGAAGCAAAGGTATTAGTTGATTTGGCTGAAGAGTCTGATGAGATATACAAATTCATTATATCTGATTTGAGGCGCACTCGTATGGCAGCCATTGCAATGAAGAAATCAAATTTAGCTATCAGTGATGGAGTTTCTCCTTTGGAATCTGGCCAAAATGTCAACCAAAATTGCTTGTCGGAGGAAGAAAGTGAACCAGAATTGACTATTAGAAATCCACGCAATGCACAAACAAAAGGGAGAAAGAAGAATGGACAGAAAAACACTCAAAATTGTAGATTCAAAAGTGGAATTGAACTGTCACATGCTCAACAAGTAGCAGTCAAAAGAAAAGCATGTCATGAGTGTGGTGAATCTGGTCACAACAGCAGAACTTGCAAGAAAAGAAATCAAAATGATTAA

Protein sequence

>Msa1444710

MDSTVHWSIMDGNVFEEATQSTNVEISEEDILAAAPNVGEELNSEPCKGMEFKSVKEFYNSFAKRMGFGVRVRSTKPKRAVFVCCNEGHHIVKSSINKEIQDDTNQTKRKCSTSRTGCLATLVVKRGNWIICSFKNDHNHPMVSPKSVSYMRCHKSMNGAAKSLVEHFKEDEDWSRLMIEYGLDNNEWLQGESWIPVYNRSCFFAGMNTSQRSESINAFFDSFVNATTTLQEFVLKFEKAVDSRLEVEKREDYESRHKKRILSTGSKLEEHAAHILVIFQAKGITQIPDHFILQRCTKNANKSIEVCDTTSNFEGQNSTPKILRRMHAQQEAKVLVDLAEESDEIYKFIISDLRRTRMAAIAMKKSNLAISDGVSPLESGQNVNQNCLSEEESEPELTIRNPRNAQTKGRKKNGQKNTQNCRFKSGIELSHAQQVAVKRKACHECGESGHNSRTCKKRNQND*