AlfalfaGEDB | Msa1446070

M. sativa cultivar zhongmu-4 / Msa1446070

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
PFAM: PF07859.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1446070	sp\|Q9LVB8\|CXE20_ARATH	51.084	323	154	3	7	326	4	325	1.48e-115	340

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1446070	A0A072UAU3	96.000	325	13	0	1	325	1	325	0.0	649

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0151220	Msa1446070	0.814337	1.621544e-51	-8.615850e-47
Msa0225840	Msa1446070	0.807977	3.883696e-50	-8.615850e-47
Msa0260530	Msa1446070	0.825703	4.057371e-54	-8.615850e-47
Msa0487250	Msa1446070	0.915401	6.151302e-85	-8.615850e-47
Msa0584580	Msa1446070	0.802946	4.405954e-49	-8.615850e-47
Msa0615210	Msa1446070	0.828077	1.098580e-54	-8.615850e-47
Msa1420930	Msa1446070	0.818311	2.094304e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1446070	MtrunA17_Chr6g0474491	96.000	325	13	0	1	325	1	325	0.0	649
Msa1446070	MtrunA17_Chr6g0474471	84.848	330	49	1	1	329	1	330	0.0	578
Msa1446070	MtrunA17_Chr6g0474411	67.890	327	98	5	2	325	5	327	2.22e-159	448
Msa1446070	MtrunA17_Chr1g0204201	52.711	332	146	4	1	326	1	327	3.14e-115	336
Msa1446070	MtrunA17_Chr6g0474541	55.663	309	131	5	7	314	36	339	2.72e-112	330
Msa1446070	MtrunA17_Chr1g0204211	52.229	314	141	3	12	323	31	337	1.37e-109	322
Msa1446070	MtrunA17_Chr6g0474631	49.085	328	157	5	2	326	6	326	6.44e-102	302
Msa1446070	MtrunA17_Chr8g0351201	48.802	334	155	5	2	328	4	328	1.26e-101	301
Msa1446070	MtrunA17_Chr8g0351211	48.160	326	149	6	12	326	2	318	4.34e-97	290
Msa1446070	MtrunA17_Chr8g0351171	37.188	320	187	5	12	324	5	317	7.21e-74	230

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1446070	AT5G62180.1	51.084	323	154	3	7	326	4	325	1.51e-116	340
Msa1446070	AT2G45600.1	43.976	332	156	6	7	326	4	317	1.02e-91	277
Msa1446070	AT2G45610.1	40.854	328	175	6	8	325	6	324	1.99e-78	243
Msa1446070	AT5G16080.1	37.175	269	146	7	34	293	51	305	1.06e-47	164
Msa1446070	AT5G23530.1	33.657	309	186	7	21	324	32	326	3.63e-47	162
Msa1446070	AT1G68620.1	36.333	300	174	7	34	328	46	333	3.71e-46	160
Msa1446070	AT1G19190.1	32.734	278	156	5	24	290	18	275	5.97e-39	140
Msa1446070	AT1G49640.1	38.788	165	97	3	39	203	34	194	2.94e-36	133
Msa1446070	AT5G06570.1	35.254	295	165	9	46	327	47	328	2.35e-35	131
Msa1446070	AT5G06570.2	35.254	295	165	9	46	327	47	328	2.35e-35	131

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Find 73 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGTGGAACATAGTGG+AGG	0.668304	tig0031260:-32308	Msa1446070:intergenic
ACCATTCCCAACCCTTAGAT+TGG	0.372685	tig0031260:-32746	Msa1446070:intergenic
ACCGGAGCACCGGCTACCGG+CGG	0.597839	tig0031260:+32390	Msa1446070:CDS
AGAAGTGTCTAATGAAGGTG+AGG	0.582650	tig0031260:-32125	Msa1446070:intergenic
AGATTAAGGATCATGAGTGG+AGG	0.598392	tig0031260:+32788	Msa1446070:CDS
AGGACTACGTGTAGTTCAAA+AGG	0.533022	tig0031260:+32543	Msa1446070:CDS
AGGGTGTTGGTGAGTATGAA+TGG	0.462381	tig0031260:+32808	Msa1446070:CDS
AGTATTCCAATCTAAGGGTT+GGG	0.474229	tig0031260:+32740	Msa1446070:CDS
ATAGCATCATCATATGCCGC+CGG	0.642105	tig0031260:-32406	Msa1446070:intergenic
ATATTGACCCTAAGCATCAA+AGG	0.589799	tig0031260:-32995	Msa1446070:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCATGAAAGAAAACAATAAG+TGG	-	tig0031260:32262-32281	Msa1446070:intergenic	30.0%
ACAAAAATTGTGGAACATAG+TGG	-	tig0031260:32314-32333	Msa1446070:intergenic	30.0%
CCATTTCAGAACAAAAATTG+TGG	-	tig0031260:32324-32343	Msa1446070:intergenic	30.0%
ATCAAATCACTAACACACAA+AGG	-	tig0031260:32679-32698	Msa1446070:intergenic	30.0%
ATCATGAGTATTCCAATCTA+AGG	+	tig0031260:32734-32753	Msa1446070:CDS	30.0%
TCATGAGTATTCCAATCTAA+GGG	+	tig0031260:32735-32754	Msa1446070:CDS	30.0%
CTCAATGAAATTCTTTGCTT+TGG	-	tig0031260:32953-32972	Msa1446070:intergenic	30.0%
CAAAGCAAAGAATTTCATTG+AGG	+	tig0031260:32951-32970	Msa1446070:CDS	30.0%
CCATGAAAGAAAACAATAAG+TGG	-	tig0031260:32262-32281	Msa1446070:intergenic	30.0%
ACAAAAATTGTGGAACATAG+TGG	-	tig0031260:32314-32333	Msa1446070:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0031260	gene	32019	33008	32019	ID=Msa1446070;Name=Msa1446070
tig0031260	mRNA	32019	33008	32019	ID=Msa1446070-mRNA-1;Parent=Msa1446070;Name=Msa1446070-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|329
tig0031260	exon	32019	33008	32019	ID=Msa1446070-mRNA-1:exon:15283;Parent=Msa1446070-mRNA-1
tig0031260	CDS	32019	33008	32019	ID=Msa1446070-mRNA-1:cds;Parent=Msa1446070-mRNA-1

Gene Sequence

>Msa1446070

ATGTCAAATCAAACTTTGCCACCACCCATCAACGATCCTTACCAATACGTCAAAATTCAACACAATCCCAACGACACACTCACTCGAAATCTCGAAGATCCACACACCTCACCTTCATTAGACACTTCTCTTCCTGTTCTAACCAAAGATCTCACCATCAACCAATCAAACCAAACATGGCTTCGATTATTCCTACCTAAAAAAGCAACAAATGTTTCAAACCTAAACAACAAGCTACTACCACTTATTGTTTTCTTTCATGGAAGTGGCTTCATTGTACTAAGTGCAGCCTCCACTATGTTCCACAATTTTTGTTCTGAAATGGCGGACACCGTCGAAGCAGTTGTCGCCTCGGTCGATTATCGCCTCGCACCGGAGCACCGGCTACCGGCGGCATATGATGATGCTATGGAAGCATTGAGTTTGATTAGGTCAAGTGATGATGAATGGTTGACTAAATACGTTGACTTTTCAAAATGTTTTCTAATGGGAAATAGTGCTGGAGCTACAATTGCATATCATGCAGGACTACGTGTAGTTCAAAAGGTGAATGATCTTGAGCCGTTGAAAATTCTAGGGATGATATTGCGTCAACCATTTTTTGGTGGGACCAATAGGACTGAGTCAGAGTTAAGACTTGAGAATGATCCTGTTTTTCCTTTGTGTGTTAGTGATTTGATGTGGGAGTTAGCGTTACCAATTGGTGTTAAACGTGATCATGAGTATTCCAATCTAAGGGTTGGGAATGGTGTTGATGAAAAACTTGCTAAGATTAAGGATCATGAGTGGAGGGTGTTGGTGAGTATGAATGGTGGAGATCCATTGGTTGATCGTAATAAGGAGTTGGTTAAATTGTTGGAGGAGAAGGGTGTGGAAGTGGTGAAGGATTTTCAAGAAGATGGTTTTCATGGGGTTGAGTTTTTTGAGCCATCCAAAGCAAAGAATTTCATTGAGGTAGTAAAAGCTTTCATCTCTTCCTTTGATGCTTAG

Protein sequence

>Msa1446070

MSNQTLPPPINDPYQYVKIQHNPNDTLTRNLEDPHTSPSLDTSLPVLTKDLTINQSNQTWLRLFLPKKATNVSNLNNKLLPLIVFFHGSGFIVLSAASTMFHNFCSEMADTVEAVVASVDYRLAPEHRLPAAYDDAMEALSLIRSSDDEWLTKYVDFSKCFLMGNSAGATIAYHAGLRVVQKVNDLEPLKILGMILRQPFFGGTNRTESELRLENDPVFPLCVSDLMWELALPIGVKRDHEYSNLRVGNGVDEKLAKIKDHEWRVLVSMNGGDPLVDRNKELVKLLEEKGVEVVKDFQEDGFHGVEFFEPSKAKNFIEVVKAFISSFDA*