Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1447360 | G7KJV3 | 86.207 | 58 | 8 | 0 | 1 | 58 | 1 | 58 | 1.83e-25 | 102 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1394900 | Msa1447360 | 0.810385 | 1.183351e-50 | -8.615850e-47 |
| Msa0966360 | Msa1447360 | 0.968569 | 7.292612e-129 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1447360 | MtrunA17_Chr6g0488561 | 86.207 | 58 | 8 | 0 | 1 | 58 | 1 | 58 | 3.52e-29 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACACTTGCTTCCTTTCA+AGG | 0.304706 | tig0031706:+19131 | Msa1447360:CDS |
| CCCTCTTGGGTGAGAGTGGC+TGG | 0.350504 | tig0031706:-19080 | Msa1447360:intergenic |
| GGAAGAGAGAAACCCCTCTT+GGG | 0.525832 | tig0031706:-19093 | Msa1447360:intergenic |
| AGGAAGAGAGAAACCCCTCT+TGG | 0.530814 | tig0031706:-19094 | Msa1447360:intergenic |
| GAGGTGGAAGGAGATGAAAG+TGG | 0.533907 | tig0031706:-19042 | Msa1447360:intergenic |
| AAACCCCTCTTGGGTGAGAG+TGG | 0.557513 | tig0031706:-19084 | Msa1447360:intergenic |
| CCAGCCACTCTCACCCAAGA+GGG | 0.560649 | tig0031706:+19080 | Msa1447360:CDS |
| CTGGAGAGTAGTGAAGAAAG+AGG | 0.565133 | tig0031706:-19061 | Msa1447360:intergenic |
| GAGAGTAGTGAAGAAAGAGG+TGG | 0.607387 | tig0031706:-19058 | Msa1447360:intergenic |
| TCCAGCCACTCTCACCCAAG+AGG | 0.638518 | tig0031706:+19079 | Msa1447360:CDS |
| CAGCCACTCTCACCCAAGAG+GGG | 0.649078 | tig0031706:+19081 | Msa1447360:CDS |
| GTAGTGAAGAAAGAGGTGGA+AGG | 0.662299 | tig0031706:-19054 | Msa1447350:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATCACAATAATAAAAA+GGG | - | tig0031706:19175-19194 | Msa1447360:intergenic | 10.0% |
| !! | AAATAATCACAATAATAAAA+AGG | - | tig0031706:19176-19195 | Msa1447360:intergenic | 10.0% |
| !! | ATAATCACAATAATAAAAAG+GGG | - | tig0031706:19174-19193 | Msa1447360:intergenic | 15.0% |
| ! | AAAAAAAAAGTGACCTTGAA+AGG | - | tig0031706:19147-19166 | Msa1447360:intergenic | 25.0% |
| AAAACACTTGCTTCCTTTCA+AGG | + | tig0031706:19131-19150 | Msa1447360:CDS | 35.0% | |
| ! | GGAAGCAAGTGTTTTCTTCT+GGG | - | tig0031706:19126-19145 | Msa1447360:intergenic | 40.0% |
| ! | AGGAAGCAAGTGTTTTCTTC+TGG | - | tig0031706:19127-19146 | Msa1447360:intergenic | 40.0% |
| GTAGTGAAGAAAGAGGTGGA+AGG | - | tig0031706:19057-19076 | Msa1461920:intergenic | 45.0% | |
| GAGAGTAGTGAAGAAAGAGG+TGG | - | tig0031706:19061-19080 | Msa1461920:intergenic | 45.0% | |
| CTGGAGAGTAGTGAAGAAAG+AGG | - | tig0031706:19064-19083 | Msa1461920:intergenic | 45.0% | |
| ! | TGTTTTCTTCTGGGTGGAGA+AGG | - | tig0031706:19117-19136 | Msa1447360:intergenic | 45.0% |
| ! | AGCAAGTGTTTTCTTCTGGG+TGG | - | tig0031706:19123-19142 | Msa1447360:intergenic | 45.0% |
| GAGGTGGAAGGAGATGAAAG+TGG | - | tig0031706:19045-19064 | Msa1461920:intergenic | 50.0% | |
| GGAAGAGAGAAACCCCTCTT+GGG | - | tig0031706:19096-19115 | Msa1447360:intergenic | 50.0% | |
| AGGAAGAGAGAAACCCCTCT+TGG | - | tig0031706:19097-19116 | Msa1447360:intergenic | 50.0% | |
| AAACCCCTCTTGGGTGAGAG+TGG | - | tig0031706:19087-19106 | Msa1447360:intergenic | 55.0% | |
| TCCAGCCACTCTCACCCAAG+AGG | + | tig0031706:19079-19098 | Msa1447360:CDS | 60.0% | |
| CCAGCCACTCTCACCCAAGA+GGG | + | tig0031706:19080-19099 | Msa1447360:CDS | 60.0% | |
| CAGCCACTCTCACCCAAGAG+GGG | + | tig0031706:19081-19100 | Msa1447360:CDS | 60.0% | |
| CCCTCTTGGGTGAGAGTGGC+TGG | - | tig0031706:19083-19102 | Msa1447360:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0031706 | gene | 18985 | 19215 | 18985 | ID=Msa1447360;Name=Msa1447360 |
| tig0031706 | mRNA | 18985 | 19215 | 18985 | ID=Msa1447360-mRNA-1;Parent=Msa1447360;Name=Msa1447360-mRNA-1;_AED=0.51;_eAED=0.51;_QI=0|0|0|0.5|0|0|2|0|60 |
| tig0031706 | exon | 18985 | 19152 | 18985 | ID=Msa1447360-mRNA-1:exon:15885;Parent=Msa1447360-mRNA-1 |
| tig0031706 | exon | 19201 | 19215 | 19201 | ID=Msa1447360-mRNA-1:exon:15886;Parent=Msa1447360-mRNA-1 |
| tig0031706 | CDS | 18985 | 19152 | 18985 | ID=Msa1447360-mRNA-1:cds;Parent=Msa1447360-mRNA-1 |
| tig0031706 | CDS | 19201 | 19215 | 19201 | ID=Msa1447360-mRNA-1:cds;Parent=Msa1447360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
