AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0966360


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0966360 MtrunA17_Chr6g0488561 86.207 58 8 0 1 58 1 58 3.52e-29 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACACTTGCTTCCTTTCA+AGG 0.304706 6_3:-94552577 Msa0966360:intron
CCCTCTTGGGTGAGAGTGGC+TGG 0.350504 6_3:+94552628 None:intergenic
GGAAGAGAGAAACCCCTCTT+GGG 0.525832 6_3:+94552615 None:intergenic
AGGAAGAGAGAAACCCCTCT+TGG 0.530814 6_3:+94552614 None:intergenic
GAGGTGGAAGGAGATGAAAG+TGG 0.533907 6_3:+94552666 None:intergenic
AAACCCCTCTTGGGTGAGAG+TGG 0.557513 6_3:+94552624 None:intergenic
CCAGCCACTCTCACCCAAGA+GGG 0.560649 6_3:-94552628 Msa0966360:CDS
CTGGAGAGTAGTGAAGAAAG+AGG 0.565133 6_3:+94552647 None:intergenic
GAGAGTAGTGAAGAAAGAGG+TGG 0.607387 6_3:+94552650 None:intergenic
TCCAGCCACTCTCACCCAAG+AGG 0.638518 6_3:-94552629 Msa0966360:CDS
CAGCCACTCTCACCCAAGAG+GGG 0.649078 6_3:-94552627 Msa0966360:CDS
GTAGTGAAGAAAGAGGTGGA+AGG 0.662299 6_3:+94552654 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATCACAATAATAAAAA+GGG + chr6_3:94552705-94552724 None:intergenic 10.0%
!! AAATAATCACAATAATAAAA+AGG + chr6_3:94552706-94552725 None:intergenic 10.0%
!! ATAATCACAATAATAAAAAG+GGG + chr6_3:94552704-94552723 None:intergenic 15.0%
! AAAAAAAAAGTGACCTTGAA+AGG + chr6_3:94552677-94552696 None:intergenic 25.0%
AAAACACTTGCTTCCTTTCA+AGG - chr6_3:94552661-94552680 Msa0966360:CDS 35.0%
! GGAAGCAAGTGTTTTCTTCT+GGG + chr6_3:94552656-94552675 None:intergenic 40.0%
! AGGAAGCAAGTGTTTTCTTC+TGG + chr6_3:94552657-94552676 None:intergenic 40.0%
GTAGTGAAGAAAGAGGTGGA+AGG + chr6_3:94552587-94552606 None:intergenic 45.0%
GAGAGTAGTGAAGAAAGAGG+TGG + chr6_3:94552591-94552610 None:intergenic 45.0%
CTGGAGAGTAGTGAAGAAAG+AGG + chr6_3:94552594-94552613 None:intergenic 45.0%
! TGTTTTCTTCTGGGTGGAGA+AGG + chr6_3:94552647-94552666 None:intergenic 45.0%
! AGCAAGTGTTTTCTTCTGGG+TGG + chr6_3:94552653-94552672 None:intergenic 45.0%
GAGGTGGAAGGAGATGAAAG+TGG + chr6_3:94552575-94552594 None:intergenic 50.0%
GGAAGAGAGAAACCCCTCTT+GGG + chr6_3:94552626-94552645 None:intergenic 50.0%
AGGAAGAGAGAAACCCCTCT+TGG + chr6_3:94552627-94552646 None:intergenic 50.0%
AAACCCCTCTTGGGTGAGAG+TGG + chr6_3:94552617-94552636 None:intergenic 55.0%
TCCAGCCACTCTCACCCAAG+AGG - chr6_3:94552609-94552628 Msa0966360:CDS 60.0%
CCAGCCACTCTCACCCAAGA+GGG - chr6_3:94552610-94552629 Msa0966360:CDS 60.0%
CAGCCACTCTCACCCAAGAG+GGG - chr6_3:94552611-94552630 Msa0966360:CDS 60.0%
CCCTCTTGGGTGAGAGTGGC+TGG + chr6_3:94552613-94552632 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 94552515 94552745 94552515 ID=Msa0966360;Name=Msa0966360
chr6_3 mRNA 94552515 94552745 94552515 ID=Msa0966360-mRNA-1;Parent=Msa0966360;Name=Msa0966360-mRNA-1;_AED=0.51;_eAED=0.51;_QI=0|0|0|0.5|0|0|2|0|60
chr6_3 exon 94552578 94552745 94552578 ID=Msa0966360-mRNA-1:exon:17486;Parent=Msa0966360-mRNA-1
chr6_3 exon 94552515 94552529 94552515 ID=Msa0966360-mRNA-1:exon:17485;Parent=Msa0966360-mRNA-1
chr6_3 CDS 94552578 94552745 94552578 ID=Msa0966360-mRNA-1:cds;Parent=Msa0966360-mRNA-1
chr6_3 CDS 94552515 94552529 94552515 ID=Msa0966360-mRNA-1:cds;Parent=Msa0966360-mRNA-1
Gene Sequence

>Msa0966360

ATGGCACTACATGTTCATAATACTATTCGTTTACACTTTCAATATCAATATCTTCTTCCACTTTCATCTCCTTCCACCTCTTTCTTCACTACTCTCCAGCCACTCTCACCCAAGAGGGGTTTCTCTCTTCCTTCTCCACCCAGAAGAAAACACTTGCTTCCTTTCAAGTTTTTACATGATTAG

Protein sequence

>Msa0966360

MALHVHNTIRLHFQYQYLLPLSSPSTSFFTTLQPLSPKRGFSLPSPPRRKHLLPFKFLHD*