AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1448240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 87 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCCATAGATTTATTTCT+TGG 0.184877 tig0032141:-4358 None:intergenic
CCATTTCACGGAATCTAATT+TGG 0.209963 tig0032141:-4829 None:intergenic
CTTCCCCATCAGTTGTTATT+TGG 0.213555 tig0032141:+4133 Msa1448240:five_prime_UTR
TTGAACAACAGTATTGTATT+TGG 0.223829 tig0032141:-4236 None:intergenic
CATATAAAACTTGATGAAAA+TGG 0.268437 tig0032141:+4779 Msa1448240:five_prime_UTR
ACGAAGGGATCTGGTGGATA+TGG 0.271051 tig0032141:-6514 None:intergenic
ATTCACTTTGATTGTCACTA+TGG 0.299236 tig0032141:+4737 Msa1448240:five_prime_UTR
TATATCTTCATGTAAATAAC+TGG 0.311314 tig0032141:-4695 None:intergenic
TTGAACAACAATATTGTATT+GGG 0.318198 tig0032141:+4090 Msa1448240:five_prime_UTR
TCTGGATGGTGGTGGGAATA+GGG 0.337237 tig0032141:-6440 None:intergenic
TGAACCAAGAAATAAATCTA+TGG 0.348089 tig0032141:+4354 Msa1448240:five_prime_UTR
ATCTGGATGGTGGTGGGAAT+AGG 0.353411 tig0032141:-6441 None:intergenic
GACGGAGCTGCTGCAGAGAT+TGG 0.357540 tig0032141:+4910 Msa1448240:CDS
ATTGAACAACAATATTGTAT+TGG 0.359038 tig0032141:+4089 Msa1448240:five_prime_UTR
ATTATAATGTCTTGCTTTGG+TGG 0.366564 tig0032141:+4437 Msa1448240:five_prime_UTR
TTTATTATAATGTCTTGCTT+TGG 0.372677 tig0032141:+4434 Msa1448240:five_prime_UTR
CCACCATCCAGATTTCGATT+TGG 0.376223 tig0032141:+6451 Msa1448240:CDS
CTGCTAGATCGGAGATGGGC+CGG 0.378849 tig0032141:+4887 Msa1448240:CDS
CCAAATTAGATTCCGTGAAA+TGG 0.389565 tig0032141:+4829 Msa1448240:exon
GTATCTGAACCATTACTCTA+AGG 0.396743 tig0032141:+6598 Msa1448240:CDS
AAAGGATAACAAATTCTTGT+TGG 0.411653 tig0032141:+4322 Msa1448240:five_prime_UTR
AAAGTGAGGATTCATAAGTA+TGG 0.420071 tig0032141:+4486 Msa1448240:five_prime_UTR
TATTTGGATGTAATGGATGA+TGG 0.426459 tig0032141:+4188 Msa1448240:five_prime_UTR
CGGGGCTGCTAGATCGGAGA+TGG 0.432256 tig0032141:+4882 Msa1448240:CDS
TTGTACTTGCCAACGAGCTT+AGG 0.440609 tig0032141:-4392 None:intergenic
TAATACCAAATAACAACTGA+TGG 0.458535 tig0032141:-4138 None:intergenic
CCCTTCGTCAACAACATCAA+TGG 0.462313 tig0032141:+6529 Msa1448240:CDS
TACAGATCTGAATTTCTTAG+TGG 0.463536 tig0032141:-6578 None:intergenic
TATAAGATATTATCCTTCAA+AGG 0.473293 tig0032141:-4540 None:intergenic
GATGGGGAAGAGATTCTAAC+AGG 0.478017 tig0032141:+4206 Msa1448240:five_prime_UTR
CTCATGCAAGAAACGGTTGA+TGG 0.480355 tig0032141:+5444 Msa1448240:CDS
CGAAGGGATCTGGTGGATAT+GGG 0.481452 tig0032141:-6513 None:intergenic
GAATGAACACAATCCTTTGA+AGG 0.481506 tig0032141:+4527 Msa1448240:five_prime_UTR
GTGGTAGCCAAATCGAAATC+TGG 0.482491 tig0032141:-6458 None:intergenic
ATGTTGTTGACGAAGGGATC+TGG 0.485241 tig0032141:-6523 None:intergenic
AAAAGATGTCCAATGTCAAA+TGG 0.485689 tig0032141:+5350 Msa1448240:CDS
TGCTAGATCGGAGATGGGCC+GGG 0.486001 tig0032141:+4888 Msa1448240:CDS
AGGTGACGGGGCTGCTAGAT+CGG 0.496214 tig0032141:+4876 Msa1448240:CDS
CCTTCGTCAACAACATCAAT+GGG 0.500127 tig0032141:+6530 Msa1448240:CDS
AAGATATAATGAATTGAGCT+TGG 0.509519 tig0032141:+4710 Msa1448240:five_prime_UTR
AATACCAAATAACAACTGAT+GGG 0.512209 tig0032141:-4137 None:intergenic
ATTTGGATGTAATGGATGAT+GGG 0.513339 tig0032141:+4189 Msa1448240:five_prime_UTR
TAATAAAGCTAGCAGGCCTA+TGG 0.514573 tig0032141:-4418 None:intergenic
GACATTATAATAAAGCTAGC+AGG 0.524437 tig0032141:-4425 None:intergenic
CTGAATATGTTGAAGTGAAA+GGG 0.524733 tig0032141:+5374 Msa1448240:CDS
CCCATTGATGTTGTTGACGA+AGG 0.526146 tig0032141:-6530 None:intergenic
GGATCGGTACCTTAGAGTAA+TGG 0.529403 tig0032141:-6607 None:intergenic
TTCCTCGCTCATGCAAGAAA+CGG 0.534516 tig0032141:+5437 Msa1448240:CDS
AAGTGAACCTTCTGAGTAGA+GGG 0.535875 tig0032141:-6402 None:intergenic
CCATTGATGTTGTTGACGAA+GGG 0.542663 tig0032141:-6529 None:intergenic
ATAAATCTATGGAATATTGA+AGG 0.543789 tig0032141:+4365 Msa1448240:five_prime_UTR
ATATTCAGACCATTTGACAT+TGG 0.548689 tig0032141:-5359 None:intergenic
AATCGAAATCTGGATGGTGG+TGG 0.550997 tig0032141:-6448 None:intergenic
GAGGCTCTCTCTGTTGCTCT+CGG 0.552078 tig0032141:+6552 Msa1448240:CDS
AGTTGCGCAAAGGAGGTGAC+GGG 0.556812 tig0032141:+4863 Msa1448240:CDS
AAGTTGCGCAAAGGAGGTGA+CGG 0.559484 tig0032141:+4862 Msa1448240:CDS
TCTGAATATGTTGAAGTGAA+AGG 0.563055 tig0032141:+5373 Msa1448240:CDS
TCTCTGCAGCAGCTCCGTCC+CGG 0.570077 tig0032141:-4906 None:intergenic
GCTGCTGCAGAGATTGGAGA+TGG 0.571294 tig0032141:+4916 Msa1448240:CDS
TTTCATTGCTAGTGAAAGTG+AGG 0.572779 tig0032141:+4472 Msa1448240:five_prime_UTR
CTTCAAAAGTTGCGCAAAGG+AGG 0.573380 tig0032141:+4856 Msa1448240:CDS
AACCGTTTCTTGCATGAGCG+AGG 0.574230 tig0032141:-5439 None:intergenic
GTTGTTCAATAAAGAAACAA+AGG 0.577442 tig0032141:-4076 None:intergenic
GGCTGTAGCAAGAGGAGTGA+TGG 0.581001 tig0032141:-6488 None:intergenic
ATCGAAATCTGGATGGTGGT+GGG 0.583453 tig0032141:-6447 None:intergenic
AGATCGGAGATGGGCCGGGA+CGG 0.593534 tig0032141:+4892 Msa1448240:CDS
ACAGATCTGAATTTCTTAGT+GGG 0.596428 tig0032141:-6577 None:intergenic
TTGGCAAGTACAATGGCCAT+AGG 0.598301 tig0032141:+4402 Msa1448240:five_prime_UTR
GAGGAGTGATGGTGGTGATG+TGG 0.599738 tig0032141:-6477 None:intergenic
GGGGCTGCTAGATCGGAGAT+GGG 0.600787 tig0032141:+4883 Msa1448240:CDS
GTTGTTCAATAAAGAAACAA+AGG 0.604315 tig0032141:+4250 Msa1448240:five_prime_UTR
TACCTTCAAAAGTTGCGCAA+AGG 0.612577 tig0032141:+4853 Msa1448240:CDS
TATGGGTTGGCTGTAGCAAG+AGG 0.625430 tig0032141:-6496 None:intergenic
GAAGGCGATCCTAAGCTCGT+TGG 0.625460 tig0032141:+4383 Msa1448240:five_prime_UTR
GAAGTGAACCTTCTGAGTAG+AGG 0.627037 tig0032141:-6403 None:intergenic
GGGATCTGGTGGATATGGGT+TGG 0.633128 tig0032141:-6509 None:intergenic
GTGAACCTTCTGAGTAGAGG+GGG 0.639709 tig0032141:-6400 None:intergenic
TTTGGATGTAATGGATGATG+GGG 0.641334 tig0032141:+4190 Msa1448240:five_prime_UTR
AAGCTCGTTGGCAAGTACAA+TGG 0.642578 tig0032141:+4395 Msa1448240:five_prime_UTR
TAGCCAAATCGAAATCTGGA+TGG 0.656178 tig0032141:-6454 None:intergenic
ATACCAAATAACAACTGATG+GGG 0.658683 tig0032141:-4136 None:intergenic
CCAAATCGAAATCTGGATGG+TGG 0.661869 tig0032141:-6451 None:intergenic
TTGTTGACGAAGGGATCTGG+TGG 0.671155 tig0032141:-6520 None:intergenic
TCGTCAACAACATCAATGGG+AGG 0.687792 tig0032141:+6533 Msa1448240:CDS
GTTGCGCAAAGGAGGTGACG+GGG 0.703235 tig0032141:+4864 Msa1448240:CDS
TGTAGCAAGAGGAGTGATGG+TGG 0.703527 tig0032141:-6485 None:intergenic
AGTGAACCTTCTGAGTAGAG+GGG 0.704831 tig0032141:-6401 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAATCTAATACAA+CGG - tig0032141:4173-4192 None:intergenic 10.0%
!! TATCTACAAATATAAAAAAT+AGG + tig0032141:5559-5578 Msa1448240:intron 10.0%
!!! ATTTTTATATATCGATTATA+TGG - tig0032141:6119-6138 None:intergenic 10.0%
!! ATATAATCGATATATAAAAA+TGG + tig0032141:6118-6137 Msa1448240:intron 10.0%
!!! GTTGTATTAGATTTTTTATT+TGG + tig0032141:4172-4191 Msa1448240:five_prime_UTR 15.0%
!!! AATCAAGTTTTTATGATATA+AGG - tig0032141:4636-4655 None:intergenic 15.0%
!!! ATTCTAAAAAACAATTTTTG+TGG - tig0032141:5028-5047 None:intergenic 15.0%
!! AGATAAATATGAAGTTAAAT+AGG - tig0032141:5544-5563 None:intergenic 15.0%
!!! CGATAACTATTTTATTTATT+TGG + tig0032141:6058-6077 Msa1448240:intron 15.0%
!!! TATTTGGATCTTTAAATTTT+AGG + tig0032141:6074-6093 Msa1448240:intron 15.0%
!!! ATTTGGATCTTTAAATTTTA+GGG + tig0032141:6075-6094 Msa1448240:intron 15.0%
!! AAACTAAATGAATAATTTCA+GGG - tig0032141:6207-6226 None:intergenic 15.0%
!! ATTGAACAACAATATTGTAT+TGG + tig0032141:4089-4108 Msa1448240:five_prime_UTR 20.0%
!! TTGAACAACAATATTGTATT+GGG + tig0032141:4090-4109 Msa1448240:five_prime_UTR 20.0%
!!! GATTTTTTATTTGGATGTAA+TGG + tig0032141:4181-4200 Msa1448240:five_prime_UTR 20.0%
!!! AACTTCTTTTTCTTTATCAA+AGG + tig0032141:4304-4323 Msa1448240:five_prime_UTR 20.0%
!!! TATTCCATAGATTTATTTCT+TGG - tig0032141:4361-4380 None:intergenic 20.0%
!! ATAAATCTATGGAATATTGA+AGG + tig0032141:4365-4384 Msa1448240:five_prime_UTR 20.0%
!! TTTATTATAATGTCTTGCTT+TGG + tig0032141:4434-4453 Msa1448240:five_prime_UTR 20.0%
!! TATAAGATATTATCCTTCAA+AGG - tig0032141:4543-4562 None:intergenic 20.0%
!! ATACTTGCTCAAAAAAAAAA+AGG - tig0032141:4606-4625 None:intergenic 20.0%
!! TATATCTTCATGTAAATAAC+TGG - tig0032141:4698-4717 None:intergenic 20.0%
!! CATATAAAACTTGATGAAAA+TGG + tig0032141:4779-4798 Msa1448240:five_prime_UTR 20.0%
!!! AAAAAACAATTTTTGTGGAT+GGG - tig0032141:5023-5042 None:intergenic 20.0%
!!! TAAAAAACAATTTTTGTGGA+TGG - tig0032141:5024-5043 None:intergenic 20.0%
!! TGGCATAATAATATAATTGA+AGG + tig0032141:5110-5129 Msa1448240:intron 20.0%
!! CAATAACACATTTAAAGATA+AGG - tig0032141:5315-5334 None:intergenic 20.0%
!! AATAATGCCTAGTAATTTAT+TGG + tig0032141:5743-5762 Msa1448240:intron 20.0%
!! TTTCTTTAATATGTTATGTG+TGG + tig0032141:5815-5834 Msa1448240:intron 20.0%
!!! TTTATATATCGATTATATGG+AGG - tig0032141:6116-6135 None:intergenic 20.0%
!! GAAACTAAATGAATAATTTC+AGG - tig0032141:6208-6227 None:intergenic 20.0%
! GTTGTTCAATAAAGAAACAA+AGG - tig0032141:4250-4269 None:intergenic 25.0%
! AATACCAAATAACAACTGAT+GGG - tig0032141:4140-4159 None:intergenic 25.0%
! TAATACCAAATAACAACTGA+TGG - tig0032141:4141-4160 None:intergenic 25.0%
! TTGAACAACAGTATTGTATT+TGG - tig0032141:4239-4258 None:intergenic 25.0%
! GTTGTTCAATAAAGAAACAA+AGG + tig0032141:4250-4269 Msa1448240:five_prime_UTR 25.0%
! AAAGGATAACAAATTCTTGT+TGG + tig0032141:4322-4341 Msa1448240:five_prime_UTR 25.0%
! TGAACCAAGAAATAAATCTA+TGG + tig0032141:4354-4373 Msa1448240:five_prime_UTR 25.0%
! AAGATATAATGAATTGAGCT+TGG + tig0032141:4710-4729 Msa1448240:five_prime_UTR 25.0%
! ATCAAAGTCAAAAATGTCAA+GGG + tig0032141:4803-4822 Msa1448240:five_prime_UTR 25.0%
! TCGATAAAGATATCTACATT+TGG - tig0032141:5076-5095 None:intergenic 25.0%
!!! AATATAATTGAAGGCACTAA+TGG + tig0032141:5119-5138 Msa1448240:intron 25.0%
! TGGCAAAATTAAATAAGAGA+AGG + tig0032141:5139-5158 Msa1448240:intron 25.0%
!! GATTAATTACCTTTGAAAGA+AGG - tig0032141:5243-5262 None:intergenic 25.0%
!!! ATAATGATGGAAACTTGTTA+TGG - tig0032141:5617-5636 None:intergenic 25.0%
! TAACAAGTTTCCATCATTAT+AGG + tig0032141:5617-5636 Msa1448240:intron 25.0%
! TTTAATATGTTATGTGTGGA+AGG + tig0032141:5819-5838 Msa1448240:intron 25.0%
! TTGATAGAAAATCTAGACAT+AGG - tig0032141:6178-6197 None:intergenic 25.0%
ATACCAAATAACAACTGATG+GGG - tig0032141:4139-4158 None:intergenic 30.0%
!! TATTTGGATGTAATGGATGA+TGG + tig0032141:4188-4207 Msa1448240:five_prime_UTR 30.0%
!! ATTTGGATGTAATGGATGAT+GGG + tig0032141:4189-4208 Msa1448240:five_prime_UTR 30.0%
GACATTATAATAAAGCTAGC+AGG - tig0032141:4428-4447 None:intergenic 30.0%
ATTATAATGTCTTGCTTTGG+TGG + tig0032141:4437-4456 Msa1448240:five_prime_UTR 30.0%
AAAGTGAGGATTCATAAGTA+TGG + tig0032141:4486-4505 Msa1448240:five_prime_UTR 30.0%
ATTCACTTTGATTGTCACTA+TGG + tig0032141:4737-4756 Msa1448240:five_prime_UTR 30.0%
CATCAAAGTCAAAAATGTCA+AGG + tig0032141:4802-4821 Msa1448240:five_prime_UTR 30.0%
TGCATGATCATAGTATACTT+TGG - tig0032141:4982-5001 None:intergenic 30.0%
GTAATTGATAGATATGCTCT+TGG + tig0032141:5161-5180 Msa1448240:intron 30.0%
CAGAGTTCAATCGTAATTAA+TGG - tig0032141:5199-5218 None:intergenic 30.0%
! TGTGTTATTGAATTGTGTAG+TGG + tig0032141:5325-5344 Msa1448240:intron 30.0%
AAAAGATGTCCAATGTCAAA+TGG + tig0032141:5350-5369 Msa1448240:CDS 30.0%
ATATTCAGACCATTTGACAT+TGG - tig0032141:5362-5381 None:intergenic 30.0%
TCTGAATATGTTGAAGTGAA+AGG + tig0032141:5373-5392 Msa1448240:CDS 30.0%
CTGAATATGTTGAAGTGAAA+GGG + tig0032141:5374-5393 Msa1448240:CDS 30.0%
ACTATCTCCTTTGTTAATAG+GGG - tig0032141:5495-5514 None:intergenic 30.0%
CACTATCTCCTTTGTTAATA+GGG - tig0032141:5496-5515 None:intergenic 30.0%
!!! AAAGGAGATAGTGGAATTTT+AGG + tig0032141:5503-5522 Msa1448240:intron 30.0%
AGTTTCCATCATTATAGGAA+TGG + tig0032141:5622-5641 Msa1448240:intron 30.0%
CTAGTGACCAATAAATTACT+AGG - tig0032141:5753-5772 None:intergenic 30.0%
ACCAATTTGAATTACCATAG+AGG + tig0032141:5926-5945 Msa1448240:intron 30.0%
TCCTCTATGGTAATTCAAAT+TGG - tig0032141:5930-5949 None:intergenic 30.0%
AGAATCAATTTCATCCTCTA+TGG - tig0032141:5943-5962 None:intergenic 30.0%
! TAGAGGATGAAATTGATTCT+AGG + tig0032141:5943-5962 Msa1448240:intron 30.0%
GACAAGAGTAACATTAAGTT+GGG - tig0032141:6250-6269 None:intergenic 30.0%
ACAGATCTGAATTTCTTAGT+GGG - tig0032141:6580-6599 None:intergenic 30.0%
TACAGATCTGAATTTCTTAG+TGG - tig0032141:6581-6600 None:intergenic 30.0%
!! TTTGGATGTAATGGATGATG+GGG + tig0032141:4190-4209 Msa1448240:five_prime_UTR 35.0%
TTTCATTGCTAGTGAAAGTG+AGG + tig0032141:4472-4491 Msa1448240:five_prime_UTR 35.0%
GAATGAACACAATCCTTTGA+AGG + tig0032141:4527-4546 Msa1448240:five_prime_UTR 35.0%
CCATTTCACGGAATCTAATT+TGG - tig0032141:4832-4851 None:intergenic 35.0%
CCAAATTAGATTCCGTGAAA+TGG + tig0032141:4829-4848 Msa1448240:exon 35.0%
!! TTTTGAAGGTAGCCATTTCA+CGG - tig0032141:4844-4863 None:intergenic 35.0%
! TATCGAGTTTGAAGCACTAA+TGG + tig0032141:5090-5109 Msa1448240:intron 35.0%
GGCAATTGTAGAAACAGTTA+GGG - tig0032141:5222-5241 None:intergenic 35.0%
AGGCAATTGTAGAAACAGTT+AGG - tig0032141:5223-5242 None:intergenic 35.0%
CTACAATTGCCTTCTTTCAA+AGG + tig0032141:5231-5250 Msa1448240:intron 35.0%
!! CGGTTGATGGTAAGAAAAAT+AGG + tig0032141:5457-5476 Msa1448240:intron 35.0%
CCACTATCTCCTTTGTTAAT+AGG - tig0032141:5497-5516 None:intergenic 35.0%
CCTATTAACAAAGGAGATAG+TGG + tig0032141:5494-5513 Msa1448240:intron 35.0%
GCTATTGAAGTCCTGATTAT+TGG + tig0032141:5648-5667 Msa1448240:intron 35.0%
TATATCAGGGTCCAATAATC+AGG - tig0032141:5662-5681 None:intergenic 35.0%
CAGCATGAGTTTATCAACTA+CGG + tig0032141:5711-5730 Msa1448240:intron 35.0%
AGATCCTTCTTTCCAATACA+GGG - tig0032141:5975-5994 None:intergenic 35.0%
AAGATCCTTCTTTCCAATAC+AGG - tig0032141:5976-5995 None:intergenic 35.0%
AAAGAAGGATCTTCAAGACT+TGG + tig0032141:5983-6002 Msa1448240:intron 35.0%
! TATCGATTATATGGAGGACT+AGG - tig0032141:6110-6129 None:intergenic 35.0%
GGACAAGAGTAACATTAAGT+TGG - tig0032141:6251-6270 None:intergenic 35.0%
TCTCTCTCTAACTTTCTATC+AGG - tig0032141:6272-6291 None:intergenic 35.0%
TGATAGAAAGTTAGAGAGAG+AGG + tig0032141:6271-6290 Msa1448240:intron 35.0%
! CTTCCCCATCAGTTGTTATT+TGG + tig0032141:4133-4152 Msa1448240:five_prime_UTR 40.0%
TAATAAAGCTAGCAGGCCTA+TGG - tig0032141:4421-4440 None:intergenic 40.0%
TACCTTCAAAAGTTGCGCAA+AGG + tig0032141:4853-4872 Msa1448240:CDS 40.0%
! GATCGCGACATAACTGTTTT+TGG + tig0032141:4938-4957 Msa1448240:CDS 40.0%
TCTCCTTTGTTAATAGGGGT+GGG - tig0032141:5491-5510 None:intergenic 40.0%
ATCTCCTTTGTTAATAGGGG+TGG - tig0032141:5492-5511 None:intergenic 40.0%
GCATGCCATTCCTATAATGA+TGG - tig0032141:5630-5649 None:intergenic 40.0%
!! CGTAGTTGATAAACTCATGC+TGG - tig0032141:5713-5732 None:intergenic 40.0%
AGGATCTTCAAGACTTGGAT+TGG + tig0032141:5988-6007 Msa1448240:intron 40.0%
! TCTTCAAGACTTGGATTGGA+AGG + tig0032141:5992-6011 Msa1448240:intron 40.0%
! TGGATTGGAAGGCATAGAAA+AGG + tig0032141:6003-6022 Msa1448240:intron 40.0%
TAAGGGAAAACCTAGAGAGA+AGG - tig0032141:6302-6321 None:intergenic 40.0%
AAGTGAACCTTCTGAGTAGA+GGG - tig0032141:6405-6424 None:intergenic 40.0%
TAGCCAAATCGAAATCTGGA+TGG - tig0032141:6457-6476 None:intergenic 40.0%
CCATTGATGTTGTTGACGAA+GGG - tig0032141:6532-6551 None:intergenic 40.0%
CCTTCGTCAACAACATCAAT+GGG + tig0032141:6530-6549 Msa1448240:CDS 40.0%
GATGGGGAAGAGATTCTAAC+AGG + tig0032141:4206-4225 Msa1448240:five_prime_UTR 45.0%
TTGTACTTGCCAACGAGCTT+AGG - tig0032141:4395-4414 None:intergenic 45.0%
AAGCTCGTTGGCAAGTACAA+TGG + tig0032141:4395-4414 Msa1448240:five_prime_UTR 45.0%
TTGGCAAGTACAATGGCCAT+AGG + tig0032141:4402-4421 Msa1448240:five_prime_UTR 45.0%
! CTCCTTTGCGCAACTTTTGA+AGG - tig0032141:4858-4877 None:intergenic 45.0%
CTTCAAAAGTTGCGCAAAGG+AGG + tig0032141:4856-4875 Msa1448240:CDS 45.0%
TTCCTCGCTCATGCAAGAAA+CGG + tig0032141:5437-5456 Msa1448240:CDS 45.0%
CTCATGCAAGAAACGGTTGA+TGG + tig0032141:5444-5463 Msa1448240:CDS 45.0%
GTTCCCACCCCTATTAACAA+AGG + tig0032141:5485-5504 Msa1448240:intron 45.0%
TCTAGGTAGTCTCCCTGTAT+TGG + tig0032141:5960-5979 Msa1448240:intron 45.0%
GTCTCCCTGTATTGGAAAGA+AGG + tig0032141:5968-5987 Msa1448240:intron 45.0%
AGGTCTCCAATGATATGGAG+GGG - tig0032141:6337-6356 None:intergenic 45.0%
GAGGTCTCCAATGATATGGA+GGG - tig0032141:6338-6357 None:intergenic 45.0%
AGTGAACCTTCTGAGTAGAG+GGG - tig0032141:6404-6423 None:intergenic 45.0%
GAAGTGAACCTTCTGAGTAG+AGG - tig0032141:6406-6425 None:intergenic 45.0%
! ATCGAAATCTGGATGGTGGT+GGG - tig0032141:6450-6469 None:intergenic 45.0%
! AATCGAAATCTGGATGGTGG+TGG - tig0032141:6451-6470 None:intergenic 45.0%
CCAAATCGAAATCTGGATGG+TGG - tig0032141:6454-6473 None:intergenic 45.0%
CCACCATCCAGATTTCGATT+TGG + tig0032141:6451-6470 Msa1448240:CDS 45.0%
GTGGTAGCCAAATCGAAATC+TGG - tig0032141:6461-6480 None:intergenic 45.0%
ATGTTGTTGACGAAGGGATC+TGG - tig0032141:6526-6545 None:intergenic 45.0%
CCCTTCGTCAACAACATCAA+TGG + tig0032141:6529-6548 Msa1448240:CDS 45.0%
CCCATTGATGTTGTTGACGA+AGG - tig0032141:6533-6552 None:intergenic 45.0%
TCGTCAACAACATCAATGGG+AGG + tig0032141:6533-6552 Msa1448240:CDS 45.0%
!!! TTTGAAAAATAAAATTATTT+TGG + tig0032141:4564-4583 Msa1448240:five_prime_UTR 5.0%
!!! TAATTTTATTTTTTATATCA+GGG - tig0032141:5675-5694 None:intergenic 5.0%
!!! TTAATTTTATTTTTTATATC+AGG - tig0032141:5676-5695 None:intergenic 5.0%
AAGTTGCGCAAAGGAGGTGA+CGG + tig0032141:4862-4881 Msa1448240:CDS 50.0%
AACCGTTTCTTGCATGAGCG+AGG - tig0032141:5442-5461 None:intergenic 50.0%
AGGGGCGGAACATAAGCTAA+GGG - tig0032141:6319-6338 None:intergenic 50.0%
TCTCCAATGATATGGAGGGG+CGG - tig0032141:6334-6353 None:intergenic 50.0%
GGAGGTCTCCAATGATATGG+AGG - tig0032141:6339-6358 None:intergenic 50.0%
AGAGGGGAAAACCTCCATTG+CGG - tig0032141:6367-6386 None:intergenic 50.0%
! GGTTTTCCCCTCTGCAATAG+AGG + tig0032141:6374-6393 Msa1448240:intron 50.0%
GTGAACCTTCTGAGTAGAGG+GGG - tig0032141:6403-6422 None:intergenic 50.0%
!! TCTGGATGGTGGTGGGAATA+GGG - tig0032141:6443-6462 None:intergenic 50.0%
!! ATCTGGATGGTGGTGGGAAT+AGG - tig0032141:6444-6463 None:intergenic 50.0%
TGTAGCAAGAGGAGTGATGG+TGG - tig0032141:6488-6507 None:intergenic 50.0%
!! TATGGGTTGGCTGTAGCAAG+AGG - tig0032141:6499-6518 None:intergenic 50.0%
! CGAAGGGATCTGGTGGATAT+GGG - tig0032141:6516-6535 None:intergenic 50.0%
! ACGAAGGGATCTGGTGGATA+TGG - tig0032141:6517-6536 None:intergenic 50.0%
TTGTTGACGAAGGGATCTGG+TGG - tig0032141:6523-6542 None:intergenic 50.0%
GAAGGCGATCCTAAGCTCGT+TGG + tig0032141:4383-4402 Msa1448240:five_prime_UTR 55.0%
AGTTGCGCAAAGGAGGTGAC+GGG + tig0032141:4863-4882 Msa1448240:CDS 55.0%
GCTGCTGCAGAGATTGGAGA+TGG + tig0032141:4916-4935 Msa1448240:CDS 55.0%
AGAGGCTCTGCCTTCTCTCT+AGG + tig0032141:6289-6308 Msa1448240:intron 55.0%
GAGGGGCGGAACATAAGCTA+AGG - tig0032141:6320-6339 None:intergenic 55.0%
GTTCCGCCCCTCCATATCAT+TGG + tig0032141:6328-6347 Msa1448240:intron 55.0%
GGGGGAGGTCTCCAATGATA+TGG - tig0032141:6342-6361 None:intergenic 55.0%
AAAACCTCCATTGCGGAGGG+GGG - tig0032141:6360-6379 None:intergenic 55.0%
GAAAACCTCCATTGCGGAGG+GGG - tig0032141:6361-6380 None:intergenic 55.0%
GGAAAACCTCCATTGCGGAG+GGG - tig0032141:6362-6381 None:intergenic 55.0%
GGGAAAACCTCCATTGCGGA+GGG - tig0032141:6363-6382 None:intergenic 55.0%
GGGAAGCCTCTATTGCAGAG+GGG - tig0032141:6383-6402 None:intergenic 55.0%
GGGGAAGCCTCTATTGCAGA+GGG - tig0032141:6384-6403 None:intergenic 55.0%
!! GAGGAGTGATGGTGGTGATG+TGG - tig0032141:6480-6499 None:intergenic 55.0%
GGCTGTAGCAAGAGGAGTGA+TGG - tig0032141:6491-6510 None:intergenic 55.0%
! GGGATCTGGTGGATATGGGT+TGG - tig0032141:6512-6531 None:intergenic 55.0%
GAGGCTCTCTCTGTTGCTCT+CGG + tig0032141:6552-6571 Msa1448240:CDS 55.0%
GTTGCGCAAAGGAGGTGACG+GGG + tig0032141:4864-4883 Msa1448240:CDS 60.0%
!! AGGTGACGGGGCTGCTAGAT+CGG + tig0032141:4876-4895 Msa1448240:CDS 60.0%
GGGGCTGCTAGATCGGAGAT+GGG + tig0032141:4883-4902 Msa1448240:CDS 60.0%
CTGCTAGATCGGAGATGGGC+CGG + tig0032141:4887-4906 Msa1448240:CDS 60.0%
TGCTAGATCGGAGATGGGCC+GGG + tig0032141:4888-4907 Msa1448240:CDS 60.0%
GACGGAGCTGCTGCAGAGAT+TGG + tig0032141:4910-4929 Msa1448240:CDS 60.0%
GGGGAAAACCTCCATTGCGG+AGG - tig0032141:6364-6383 None:intergenic 60.0%
GGGGGAAGCCTCTATTGCAG+AGG - tig0032141:6385-6404 None:intergenic 60.0%
GGCTTCCCCCTCTACTCAGA+AGG + tig0032141:6395-6414 Msa1448240:CDS 60.0%
CGGGGCTGCTAGATCGGAGA+TGG + tig0032141:4882-4901 Msa1448240:CDS 65.0%
AGATCGGAGATGGGCCGGGA+CGG + tig0032141:4892-4911 Msa1448240:CDS 65.0%
TCTCTGCAGCAGCTCCGTCC+CGG - tig0032141:4909-4928 None:intergenic 65.0%
GAGACCTCCCCCCTCCGCAA+TGG + tig0032141:6350-6369 Msa1448240:intron 70.0%
ACCTCCCCCCTCCGCAATGG+AGG + tig0032141:6353-6372 Msa1448240:intron 70.0%
ACCTCCATTGCGGAGGGGGG+AGG - tig0032141:6357-6376 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0032141 gene 4074 6618 4074 ID=Msa1448240;Name=Msa1448240
tig0032141 mRNA 4074 6618 4074 ID=Msa1448240-mRNA-1;Parent=Msa1448240;Name=Msa1448240-mRNA-1;_AED=0.51;_eAED=0.51;_QI=774|0|0.5|0.75|1|1|4|0|162
tig0032141 exon 4074 4983 4074 ID=Msa1448240-mRNA-1:exon:16295;Parent=Msa1448240-mRNA-1
tig0032141 exon 5045 5051 5045 ID=Msa1448240-mRNA-1:exon:16296;Parent=Msa1448240-mRNA-1
tig0032141 exon 5345 5465 5345 ID=Msa1448240-mRNA-1:exon:16297;Parent=Msa1448240-mRNA-1
tig0032141 exon 6394 6618 6394 ID=Msa1448240-mRNA-1:exon:16298;Parent=Msa1448240-mRNA-1
tig0032141 five_prime_UTR 4074 4847 4074 ID=Msa1448240-mRNA-1:five_prime_utr;Parent=Msa1448240-mRNA-1
tig0032141 CDS 4848 4983 4848 ID=Msa1448240-mRNA-1:cds;Parent=Msa1448240-mRNA-1
tig0032141 CDS 5045 5051 5045 ID=Msa1448240-mRNA-1:cds;Parent=Msa1448240-mRNA-1
tig0032141 CDS 5345 5465 5345 ID=Msa1448240-mRNA-1:cds;Parent=Msa1448240-mRNA-1
tig0032141 CDS 6394 6618 6394 ID=Msa1448240-mRNA-1:cds;Parent=Msa1448240-mRNA-1
Gene Sequence

>Msa1448240

ATGGCTACCTTCAAAAGTTGCGCAAAGGAGGTGACGGGGCTGCTAGATCGGAGATGGGCCGGGACGGAGCTGCTGCAGAGATTGGAGATGGATCGCGACATAACTGTTTTTGGTTCGATAGAACTCGCAAACCAAAAATGCAATGGTGAAAAGATGTCCAATGTCAAATGGTCTGAATATGTTGAAGTGAAAGGGAGAGTTAAATTACATGATTTCGAAAAATACATTCAAGAACTTCCTCGCTCATGCAAGAAACGGTTGATGAGGCTTCCCCCTCTACTCAGAAGGTTCACTTCGTTACTTGCTGCAACCCTATTCCCACCACCATCCAGATTTCGATTTGGCTACCACATCACCACCATCACTCCTCTTGCTACAGCCAACCCATATCCACCAGATCCCTTCGTCAACAACATCAATGGGAGGCTCTCTCTGTTGCTCTCGGTACCCACTAAGAAATTCAGATCTGTATCTGAACCATTACTCTAA
Protein sequence

>Msa1448240

MATFKSCAKEVTGLLDRRWAGTELLQRLEMDRDITVFGSIELANQKCNGEKMSNVKWSEYVEVKGRVKLHDFEKYIQELPRSCKKRLMRLPPLLRRFTSLLAATLFPPPSRFRFGYHITTITPLATANPYPPDPFVNNINGRLSLLLSVPTKKFRSVSEPLL*