Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449440 | G7J528 | 92.357 | 157 | 11 | 1 | 1 | 156 | 1 | 157 | 1.42e-99 | 293 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0014710 | Msa1449440 | 0.808340 | 3.249404e-50 | -8.615850e-47 |
| Msa0112750 | Msa1449440 | 0.807975 | 3.887514e-50 | -8.615850e-47 |
| Msa1082500 | Msa1449440 | 0.826622 | 2.452677e-54 | -8.615850e-47 |
| Msa0484780 | Msa1449440 | 0.804010 | 2.652197e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449440 | MtrunA17_Chr3g0132871 | 92.357 | 157 | 11 | 1 | 1 | 156 | 1 | 157 | 2.73e-103 | 293 |
| Msa1449440 | MtrunA17_Chr5g0427491 | 58.333 | 156 | 56 | 6 | 1 | 154 | 1 | 149 | 5.22e-47 | 150 |
| Msa1449440 | MtrunA17_Chr5g0412221 | 41.447 | 152 | 81 | 5 | 1 | 151 | 1 | 145 | 9.44e-21 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGATTCTGATTGGTCTAT+TGG | 0.221191 | tig0032566:-22679 | Msa1449440:CDS |
| GCTGTGCTTGTTCCTTGTTA+TGG | 0.307987 | tig0032566:-22586 | Msa1449440:CDS |
| CCTTGTTATGGATACAATTA+CGG | 0.333988 | tig0032566:-22574 | Msa1449440:CDS |
| GTCATTAACGTTAGAGAATC+CGG | 0.383193 | tig0032566:+22636 | None:intergenic |
| ATTCTGATTGGTCTATTGGC+TGG | 0.411940 | tig0032566:-22675 | Msa1449440:CDS |
| CTGAATCTGTTGATTCTGAT+TGG | 0.430316 | tig0032566:-22687 | Msa1449440:CDS |
| TATCTTCCGTGGTGGTTGTC+CGG | 0.431948 | tig0032566:-22940 | Msa1449440:CDS |
| AGCAACTTACTAAGCAATGT+TGG | 0.435452 | tig0032566:-22526 | Msa1449440:CDS |
| GTCCTTCACCTTTCTTGAAG+TGG | 0.442736 | tig0032566:+22798 | None:intergenic |
| GGACAACCACCACGGAAGAT+AGG | 0.443098 | tig0032566:+22942 | None:intergenic |
| TGATTGGTCTATTGGCTGGT+TGG | 0.460769 | tig0032566:-22671 | Msa1449440:CDS |
| TGAAGGACGAACGACATAGT+AGG | 0.468277 | tig0032566:-22783 | Msa1449440:CDS |
| ACAATTTCCTCATTAACAAA+AGG | 0.468595 | tig0032566:+22826 | None:intergenic |
| TGGTTGGAACCACATGGAAC+CGG | 0.473890 | tig0032566:-22655 | Msa1449440:CDS |
| TGTGCCTTCCACTTCAAGAA+AGG | 0.475243 | tig0032566:-22806 | Msa1449440:CDS |
| ATTTATTGAACACCCATTTG+AGG | 0.489364 | tig0032566:+22894 | None:intergenic |
| GCAACTTACTAAGCAATGTT+GGG | 0.510708 | tig0032566:-22525 | Msa1449440:CDS |
| TTCAGCTGATTCTGTTACGA+GGG | 0.517927 | tig0032566:-22869 | Msa1449440:CDS |
| ATACAATTACGGTGCCATCT+TGG | 0.518276 | tig0032566:-22563 | Msa1449440:CDS |
| TTTAGGACCCTCCTCCAAGA+TGG | 0.527506 | tig0032566:+22549 | None:intergenic |
| TTCACCTTTCTTGAAGTGGA+AGG | 0.534701 | tig0032566:+22802 | None:intergenic |
| GGAAGAGGCGAAAATTGATA+AGG | 0.536201 | tig0032566:-22761 | Msa1449440:CDS |
| TTACGGTGCCATCTTGGAGG+AGG | 0.558314 | tig0032566:-22557 | Msa1449440:CDS |
| CAATTACGGTGCCATCTTGG+AGG | 0.559063 | tig0032566:-22560 | Msa1449440:CDS |
| CGAACGACATAGTAGGGAAG+AGG | 0.561732 | tig0032566:-22776 | Msa1449440:CDS |
| GAAGGACGAACGACATAGTA+GGG | 0.563344 | tig0032566:-22782 | Msa1449440:CDS |
| CCGTAATTGTATCCATAACA+AGG | 0.583133 | tig0032566:+22574 | None:intergenic |
| CTTCAGCTGATTCTGTTACG+AGG | 0.586698 | tig0032566:-22870 | Msa1449440:CDS |
| TTCCACTTCAAGAAAGGTGA+AGG | 0.586751 | tig0032566:-22800 | Msa1449440:CDS |
| AATGTTGGGAACTTCTCGTA+TGG | 0.599832 | tig0032566:-22511 | Msa1449440:CDS |
| ACCACACAAGTAAATGGATG+CGG | 0.607450 | tig0032566:-22971 | None:intergenic |
| TACGGTGCCATCTTGGAGGA+GGG | 0.612756 | tig0032566:-22556 | Msa1449440:CDS |
| ATTGGCTGGTTGGAACCACA+TGG | 0.615006 | tig0032566:-22661 | Msa1449440:CDS |
| TCAACGAATTCATAATCAGA+CGG | 0.643239 | tig0032566:+22718 | None:intergenic |
| TTAGAGAATCCGGTTCCATG+TGG | 0.706061 | tig0032566:+22646 | None:intergenic |
| TTTCTACCGGACAACCACCA+CGG | 0.717006 | tig0032566:+22934 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAATTTCCTCATTAACAAA+AGG | + | tig0032566:22606-22625 | None:intergenic | 25.0% |
| AAAAGCCAAAAACCTCAAAT+GGG | - | tig0032566:22523-22542 | Msa1449440:CDS | 30.0% | |
| ATTTATTGAACACCCATTTG+AGG | + | tig0032566:22538-22557 | None:intergenic | 30.0% | |
| ! | GAGATTTCCTTTTGTTAATG+AGG | - | tig0032566:22596-22615 | Msa1449440:CDS | 30.0% |
| TCAACGAATTCATAATCAGA+CGG | + | tig0032566:22714-22733 | None:intergenic | 30.0% | |
| ! | CCTTGTTATGGATACAATTA+CGG | - | tig0032566:22855-22874 | Msa1449440:CDS | 30.0% |
| !!! | TTGCTTAGTAAGTTGCTTTT+AGG | + | tig0032566:22900-22919 | None:intergenic | 30.0% |
| ! | TGGCTTTTGATGTTTTCTAC+CGG | + | tig0032566:22511-22530 | None:intergenic | 35.0% |
| CAAAAGCCAAAAACCTCAAA+TGG | - | tig0032566:22522-22541 | Msa1449440:CDS | 35.0% | |
| !! | CTGAATCTGTTGATTCTGAT+TGG | - | tig0032566:22742-22761 | Msa1449440:CDS | 35.0% |
| !! | GTTGATTCTGATTGGTCTAT+TGG | - | tig0032566:22750-22769 | Msa1449440:CDS | 35.0% |
| GTCATTAACGTTAGAGAATC+CGG | + | tig0032566:22796-22815 | None:intergenic | 35.0% | |
| CCGTAATTGTATCCATAACA+AGG | + | tig0032566:22858-22877 | None:intergenic | 35.0% | |
| AGCAACTTACTAAGCAATGT+TGG | - | tig0032566:22903-22922 | Msa1449440:CDS | 35.0% | |
| GCAACTTACTAAGCAATGTT+GGG | - | tig0032566:22904-22923 | Msa1449440:CDS | 35.0% | |
| !! | GAACACCCATTTGAGGTTTT+TGG | + | tig0032566:22531-22550 | None:intergenic | 40.0% |
| ! | TTCAGCTGATTCTGTTACGA+GGG | - | tig0032566:22560-22579 | Msa1449440:CDS | 40.0% |
| TTCACCTTTCTTGAAGTGGA+AGG | + | tig0032566:22630-22649 | None:intergenic | 40.0% | |
| TTCCACTTCAAGAAAGGTGA+AGG | - | tig0032566:22629-22648 | Msa1449440:CDS | 40.0% | |
| GGAAGAGGCGAAAATTGATA+AGG | - | tig0032566:22668-22687 | Msa1449440:CDS | 40.0% | |
| !! | ATTCTGATTGGTCTATTGGC+TGG | - | tig0032566:22754-22773 | Msa1449440:CDS | 40.0% |
| !! | ATACAATTACGGTGCCATCT+TGG | - | tig0032566:22866-22885 | Msa1449440:CDS | 40.0% |
| AATGTTGGGAACTTCTCGTA+TGG | - | tig0032566:22918-22937 | Msa1449440:CDS | 40.0% | |
| ! | CTTTTTCCTATCTTCCGTGG+TGG | - | tig0032566:22481-22500 | Msa1449440:CDS | 45.0% |
| ! | CTTCAGCTGATTCTGTTACG+AGG | - | tig0032566:22559-22578 | Msa1449440:CDS | 45.0% |
| TGTGCCTTCCACTTCAAGAA+AGG | - | tig0032566:22623-22642 | Msa1449440:CDS | 45.0% | |
| GTCCTTCACCTTTCTTGAAG+TGG | + | tig0032566:22634-22653 | None:intergenic | 45.0% | |
| TGAAGGACGAACGACATAGT+AGG | - | tig0032566:22646-22665 | Msa1449440:CDS | 45.0% | |
| GAAGGACGAACGACATAGTA+GGG | - | tig0032566:22647-22666 | Msa1449440:CDS | 45.0% | |
| !! | TGATTGGTCTATTGGCTGGT+TGG | - | tig0032566:22758-22777 | Msa1449440:CDS | 45.0% |
| TTAGAGAATCCGGTTCCATG+TGG | + | tig0032566:22786-22805 | None:intergenic | 45.0% | |
| GCTGTGCTTGTTCCTTGTTA+TGG | - | tig0032566:22843-22862 | Msa1449440:CDS | 45.0% | |
| !! | CGGCTTTTTCCTATCTTCCG+TGG | - | tig0032566:22478-22497 | Msa1449440:CDS | 50.0% |
| TATCTTCCGTGGTGGTTGTC+CGG | - | tig0032566:22489-22508 | Msa1449440:CDS | 50.0% | |
| TTTCTACCGGACAACCACCA+CGG | + | tig0032566:22498-22517 | None:intergenic | 50.0% | |
| CGAACGACATAGTAGGGAAG+AGG | - | tig0032566:22653-22672 | Msa1449440:CDS | 50.0% | |
| ! | ATTGGCTGGTTGGAACCACA+TGG | - | tig0032566:22768-22787 | Msa1449440:CDS | 50.0% |
| TGGTTGGAACCACATGGAAC+CGG | - | tig0032566:22774-22793 | Msa1449440:CDS | 50.0% | |
| !! | CAATTACGGTGCCATCTTGG+AGG | - | tig0032566:22869-22888 | Msa1449440:CDS | 50.0% |
| ! | TTTAGGACCCTCCTCCAAGA+TGG | + | tig0032566:22883-22902 | None:intergenic | 50.0% |
| GGACAACCACCACGGAAGAT+AGG | + | tig0032566:22490-22509 | None:intergenic | 55.0% | |
| !! | TTACGGTGCCATCTTGGAGG+AGG | - | tig0032566:22872-22891 | Msa1449440:CDS | 55.0% |
| !! | TACGGTGCCATCTTGGAGGA+GGG | - | tig0032566:22873-22892 | Msa1449440:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0032566 | gene | 22471 | 22980 | 22471 | ID=Msa1449440;Name=Msa1449440 |
| tig0032566 | mRNA | 22471 | 22980 | 22471 | ID=Msa1449440-mRNA-1;Parent=Msa1449440;Name=Msa1449440-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|169 |
| tig0032566 | exon | 22471 | 22980 | 22471 | ID=Msa1449440-mRNA-1:exon:16948;Parent=Msa1449440-mRNA-1 |
| tig0032566 | CDS | 22471 | 22980 | 22471 | ID=Msa1449440-mRNA-1:cds;Parent=Msa1449440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
