AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1450700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1450700 MtrunA17_Chr7g0242951 97.133 558 14 1 2 557 1 558 0.0 1100
Msa1450700 MtrunA17_Chr2g0333221 54.286 70 32 0 24 93 66 135 4.36e-17 84.3
Msa1450700 MtrunA17_Chr3g0123861 56.061 66 29 0 28 93 82 147 4.71e-17 84.0
Msa1450700 MtrunA17_Chr4g0040501 45.631 103 45 4 27 118 3 105 1.10e-16 81.6
Msa1450700 MtrunA17_Chr2g0282741 45.918 98 47 3 27 118 3 100 1.76e-16 80.9
Msa1450700 MtrunA17_Chr6g0478651 47.619 84 36 1 28 111 68 143 1.02e-15 80.1
Msa1450700 MtrunA17_Chr2g0324921 28.839 267 159 10 27 279 3 252 4.13e-15 76.6
Msa1450700 MtrunA17_Chr3g0138471 34.177 158 89 4 27 178 3 151 8.38e-15 75.9
Msa1450700 MtrunA17_Chr1g0206121 41.860 86 50 0 4 89 11 96 1.05e-14 75.1
Msa1450700 MtrunA17_Chr7g0269841 54.545 66 29 1 30 94 28 93 1.93e-14 74.7
Msa1450700 MtrunA17_Chr4g0006391 48.571 70 34 1 27 94 3 72 2.50e-14 74.3
Msa1450700 MtrunA17_Chr2g0301881 41.176 85 49 1 20 103 91 175 2.51e-14 76.3
Msa1450700 MtrunA17_Chr2g0328581 54.098 61 28 0 29 89 76 136 3.95e-14 71.6
Msa1450700 MtrunA17_Chr2g0295831 53.226 62 29 0 28 89 110 171 5.14e-14 71.6
Msa1450700 MtrunA17_Chr2g0282761 49.275 69 33 1 27 93 3 71 1.79e-13 72.0
Msa1450700 MtrunA17_Chr1g0209551 48.684 76 31 2 28 102 16 84 5.66e-13 70.9
Msa1450700 MtrunA17_Chr7g0263971 52.941 68 30 1 24 89 365 432 7.41e-13 70.9
Msa1450700 MtrunA17_Chr1g0173931 53.125 64 29 1 30 92 40 103 8.53e-13 69.3
Msa1450700 MtrunA17_Chr2g0295821 50.000 62 31 0 28 89 104 165 9.44e-13 70.1
Msa1450700 MtrunA17_Chr6g0460411 42.500 80 39 2 27 100 1 79 2.06e-12 67.0
Msa1450700 MtrunA17_Chr2g0307061 43.939 66 37 0 30 95 112 177 2.39e-12 68.6
Msa1450700 MtrunA17_Chr6g0460461 42.500 80 39 2 27 100 1 79 2.45e-12 66.6
Msa1450700 MtrunA17_Chr7g0258061 53.125 64 29 1 30 92 24 87 2.46e-12 67.8
Msa1450700 MtrunA17_Chr2g0295361 44.578 83 44 1 10 90 19 101 4.74e-12 66.6
Msa1450700 MtrunA17_Chr5g0426951 52.459 61 28 1 30 89 85 145 5.48e-12 68.2
Msa1450700 MtrunA17_Chr3g0087741 48.529 68 34 1 28 94 89 156 6.02e-12 67.8
Msa1450700 MtrunA17_Chr8g0334811 42.683 82 44 1 30 111 16 94 6.47e-12 67.8
Msa1450700 MtrunA17_Chr5g0427221 41.333 75 43 1 19 92 39 113 7.98e-12 63.2
Msa1450700 MtrunA17_Chr6g0460381 44.928 69 32 2 27 89 2 70 9.20e-12 64.3
Msa1450700 MtrunA17_Chr4g0021141 44.928 69 37 1 25 92 19 87 1.80e-11 62.0
Msa1450700 MtrunA17_Chr3g0091361 43.662 71 38 1 26 94 3 73 3.31e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1450700 AT3G08970.1 55.826 575 232 6 2 557 1 572 0.0 603
Msa1450700 AT3G08970.2 55.826 575 232 6 2 557 1 572 0.0 603
Msa1450700 AT5G25530.2 40.984 122 70 1 27 146 3 124 5.16e-19 89.0
Msa1450700 AT4G39960.1 53.571 84 38 1 28 111 85 167 2.94e-18 87.8
Msa1450700 AT4G39960.2 53.571 84 38 1 28 111 85 167 2.94e-18 87.8
Msa1450700 AT2G22360.1 50.000 88 39 2 18 103 79 163 6.19e-18 87.0
Msa1450700 AT1G80030.4 57.143 70 30 0 24 93 71 140 1.85e-17 85.9
Msa1450700 AT1G80030.2 57.143 70 30 0 24 93 71 140 2.12e-17 85.5
Msa1450700 AT1G80030.3 57.143 70 30 0 24 93 71 140 2.12e-17 85.5
Msa1450700 AT1G80030.1 57.143 70 30 0 24 93 71 140 2.12e-17 85.5
Msa1450700 AT2G20560.1 48.000 100 44 4 27 118 3 102 3.70e-17 83.6
Msa1450700 AT5G25530.1 38.462 130 70 2 27 146 3 132 1.43e-16 81.6
Msa1450700 AT3G62600.1 46.939 98 43 3 1 94 1 93 3.73e-16 80.5
Msa1450700 AT4G28480.2 53.333 75 31 2 27 97 3 77 5.66e-16 79.0
Msa1450700 AT5G01390.3 52.857 70 31 1 27 94 3 72 7.80e-16 77.8
Msa1450700 AT5G01390.4 52.055 73 33 1 27 97 3 75 8.15e-16 77.4
Msa1450700 AT1G79940.4 37.037 108 61 2 3 103 68 175 8.48e-16 80.9
Msa1450700 AT4G28480.1 53.333 75 31 2 27 97 3 77 9.51e-16 79.3
Msa1450700 AT1G79940.2 37.037 108 61 2 3 103 68 175 1.11e-15 80.9
Msa1450700 AT1G79940.3 37.037 108 61 2 3 103 68 175 1.11e-15 80.9
Msa1450700 AT1G79940.1 37.037 108 61 2 3 103 68 175 1.11e-15 80.9
Msa1450700 AT5G01390.2 52.857 70 31 1 27 94 3 72 1.83e-15 77.4
Msa1450700 AT3G17830.1 44.828 87 40 1 24 110 59 137 2.64e-15 79.3
Msa1450700 AT3G08910.1 39.130 138 73 4 27 156 3 137 2.65e-15 77.8
Msa1450700 AT5G01390.1 52.857 70 31 1 27 94 3 72 3.17e-15 77.4
Msa1450700 AT1G10350.1 29.078 141 78 4 27 165 3 123 2.73e-13 72.0
Msa1450700 AT4G21180.1 34.906 106 62 2 5 103 70 175 3.57e-13 72.8
Msa1450700 AT4G21180.2 38.824 85 51 1 20 103 11 95 9.22e-13 71.2
Msa1450700 AT1G68370.1 48.684 76 31 2 28 102 17 85 9.34e-13 70.9
Msa1450700 AT3G12170.1 54.688 64 28 1 30 92 13 76 9.66e-13 68.9
Msa1450700 AT5G05750.1 34.109 129 70 3 28 144 114 239 1.14e-12 69.3
Msa1450700 AT5G06910.1 49.315 73 34 2 30 99 31 103 1.30e-12 68.9
Msa1450700 AT3G47940.1 43.662 71 37 1 27 94 3 73 1.30e-12 69.7
Msa1450700 AT3G12170.2 54.688 64 28 1 30 92 49 112 1.54e-12 68.9
Msa1450700 AT1G59725.1 43.662 71 37 1 27 94 3 73 1.64e-12 69.3
Msa1450700 AT5G59610.2 48.333 60 31 0 29 88 74 133 2.91e-12 67.4
Msa1450700 AT2G41000.2 33.333 93 56 1 27 113 2 94 3.53e-12 66.2
Msa1450700 AT1G59980.2 39.241 79 47 1 21 98 16 94 4.17e-12 68.2
Msa1450700 AT5G59610.1 48.333 60 31 0 29 88 74 133 4.70e-12 67.0
Msa1450700 AT5G59610.4 48.333 60 31 0 29 88 74 133 4.70e-12 67.0
Msa1450700 AT5G59610.3 48.333 60 31 0 29 88 74 133 4.79e-12 67.4
Msa1450700 AT5G59610.5 48.333 60 31 0 29 88 74 133 4.79e-12 67.4
Msa1450700 AT5G49060.2 43.243 74 42 0 19 92 90 163 5.01e-12 68.2
Msa1450700 AT5G49060.1 43.243 74 42 0 19 92 90 163 5.01e-12 68.2
Msa1450700 AT1G59980.1 39.241 79 47 1 21 98 16 94 7.16e-12 68.2
Msa1450700 AT5G03160.1 50.000 68 32 1 24 89 366 433 9.21e-12 67.8
Msa1450700 AT3G57340.1 38.095 84 51 1 28 110 113 196 1.37e-11 66.6
Msa1450700 AT3G57340.2 38.095 84 51 1 28 110 113 196 1.37e-11 66.6
Msa1450700 AT2G35720.1 47.436 78 37 1 30 103 17 94 1.60e-11 67.4
Msa1450700 AT2G41000.1 33.333 93 56 1 27 113 2 94 2.57e-11 63.2
Msa1450700 AT5G16650.1 46.970 66 34 1 28 92 11 76 3.37e-11 61.2
Msa1450700 AT5G16650.2 46.970 66 34 1 28 92 11 76 4.31e-11 62.0

Find 155 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATCAGCTTGTTCTTTCT+TGG 0.136383 tig0033038:-19173 None:intergenic
TTCTTATGTGTTTCCCTAAA+TGG 0.152263 tig0033038:-19667 None:intergenic
CTTGTAGATATTTGAATTTA+TGG 0.169499 tig0033038:-18133 None:intergenic
ATAGAGCATTAAGGTATATT+TGG 0.196698 tig0033038:+19702 Msa1450700:exon
CAACACTTTGAGTCCATTAT+AGG 0.240068 tig0033038:+18222 Msa1450700:CDS
TAAGCGGAGATATACCATTT+AGG 0.240306 tig0033038:+19653 Msa1450700:CDS
GATTGAAGAGTATTAATTAT+AGG 0.247090 tig0033038:+19886 Msa1450700:three_prime_UTR
TCTGGTGATTCAAATTCTTT+TGG 0.248938 tig0033038:+18003 Msa1450700:CDS
AGGAAGAATTATGATTTATA+TGG 0.254952 tig0033038:+17838 Msa1450700:CDS
AAAAGAACACTCCTGTAATT+TGG 0.262674 tig0033038:+18631 Msa1450700:CDS
CCCTTAGCCTTGTTCTTGTC+AGG 0.263130 tig0033038:-17409 None:intergenic
AACACATTCAGTTGCTTTCT+CGG 0.272186 tig0033038:+19203 Msa1450700:CDS
GGGAAATTCACTGTATTTAC+AGG 0.278143 tig0033038:+19586 Msa1450700:CDS
AAGCGGAGATATACCATTTA+GGG 0.291674 tig0033038:+19654 Msa1450700:CDS
AACACTTTGAGTCCATTATA+GGG 0.321085 tig0033038:+18223 Msa1450700:CDS
AGAACTCCAGTATGCGTAAT+TGG 0.327008 tig0033038:+19253 Msa1450700:CDS
CTAAATGCATTTGACAAAAC+AGG 0.333414 tig0033038:+19517 Msa1450700:CDS
TTATCTGATCATCAGATAAT+TGG 0.335644 tig0033038:+19940 Msa1450700:three_prime_UTR
TTTATGGCCTTAAAACTCTT+GGG 0.348537 tig0033038:-18117 None:intergenic
CTTGTACGGGTCGATTGTTT+TGG 0.353222 tig0033038:-16667 None:intergenic
TTTGGTTTGGATGATCTTCT+TGG 0.353735 tig0033038:+18027 Msa1450700:CDS
ATTTATGGCCTTAAAACTCT+TGG 0.369064 tig0033038:-18118 None:intergenic
GCTGGTCATCCGGGAGGTTA+TGG 0.370056 tig0033038:+17889 Msa1450700:CDS
GTCATGAAATGATATGAAAT+AGG 0.371194 tig0033038:-20016 None:intergenic
CTAAGAACACGCCAAATTAC+AGG 0.376368 tig0033038:-18642 None:intergenic
GATGCACTTCCAGCTATTGT+AGG 0.379433 tig0033038:+19016 Msa1450700:CDS
ATCTTATTCACCCTCTTTGA+GGG 0.379868 tig0033038:+18194 Msa1450700:CDS
AGGTCTCAATCATCTCAATC+TGG 0.381249 tig0033038:+18081 Msa1450700:CDS
ATCATCAGATAATTGGTAAT+AGG 0.388626 tig0033038:+19947 Msa1450700:three_prime_UTR
CCTTGCTTTGGAAGATCTAA+AGG 0.391551 tig0033038:-19278 None:intergenic
TTACCCCATTGTTGTTCCCC+TGG 0.394375 tig0033038:-17937 None:intergenic
TTTCCCTTTCCGAGGTTTGT+AGG 0.394549 tig0033038:-19569 None:intergenic
GTAATTTGGCGTGTTCTTAG+TGG 0.398833 tig0033038:+18645 Msa1450700:CDS
TATCTTATTCACCCTCTTTG+AGG 0.400350 tig0033038:+18193 Msa1450700:CDS
TGTACTCAAATAGAGCATTA+AGG 0.401998 tig0033038:+19693 Msa1450700:exon
CTAGAGCTGCCGCTTCCATT+TGG 0.402135 tig0033038:-19445 None:intergenic
AGAGCCGCCGCCAAAGGAAA+AGG 0.403748 tig0033038:-17983 None:intergenic
TCAAACTTTGAGGCTCTTTG+TGG 0.404129 tig0033038:+19226 Msa1450700:CDS
TGTATCATATGTACTCTTGT+TGG 0.405045 tig0033038:-19775 None:intergenic
TGGATTTGAAGCTGGTCATC+CGG 0.406744 tig0033038:+17879 Msa1450700:CDS
ACCAGCAACCTCCCCTATAA+TGG 0.410147 tig0033038:-18235 None:intergenic
TACACTGCAAGGAGCTTTGA+AGG 0.416754 tig0033038:+18257 Msa1450700:CDS
CAAATGGAAGCGGCAGCTCT+AGG 0.421742 tig0033038:+19446 Msa1450700:CDS
ATCTAAGAGACCATAGGAAA+TGG 0.425174 tig0033038:-19473 None:intergenic
GCTTTCTCGGTCAAACTTTG+AGG 0.426568 tig0033038:+19216 Msa1450700:CDS
CAGACCAGGGGAACAACAAT+GGG 0.426872 tig0033038:+17933 Msa1450700:CDS
CAAACTTTGAGGCTCTTTGT+GGG 0.430001 tig0033038:+19227 Msa1450700:CDS
TTCTTACAAGGAAAATGAAA+AGG 0.436187 tig0033038:+18452 Msa1450700:CDS
AAGGCACCAATTACGCATAC+TGG 0.438364 tig0033038:-19259 None:intergenic
AAGTTTGCTCAGATAAATAA+TGG 0.444421 tig0033038:+17442 Msa1450700:CDS
CCGGGAGGTTATGGTGGTCC+TGG 0.445625 tig0033038:+17898 Msa1450700:CDS
TTTGTCAAATGCATTTAGAA+AGG 0.446085 tig0033038:-19512 None:intergenic
TCTGTAAAAGATGTAAAATC+TGG 0.447024 tig0033038:+19082 Msa1450700:CDS
AGAGGTGTATCTTATTGAAC+GGG 0.447427 tig0033038:+19993 Msa1450700:three_prime_UTR
GGAAAATGAAAAGGGTTCTC+TGG 0.447913 tig0033038:+18461 Msa1450700:CDS
TTTCATATCATTTCATGACT+TGG 0.455139 tig0033038:+20020 Msa1450700:three_prime_UTR
TTAATGCTCTATTTGAGTAC+AGG 0.455543 tig0033038:-19691 None:intergenic
GGAACAACAATGGGGTAACC+AGG 0.458050 tig0033038:+17942 Msa1450700:CDS
GATCAATGTGGTGGCTGAAC+GGG 0.458955 tig0033038:-16604 None:intergenic
GGTGGAGTACGGCGGTGACT+TGG 0.466137 tig0033038:+18482 Msa1450700:CDS
GCTAGAATCACTTCTCACTT+TGG 0.471450 tig0033038:+19306 Msa1450700:CDS
TCCATTATAGGGGAGGTTGC+TGG 0.471727 tig0033038:+18234 Msa1450700:CDS
GGTAGCCAACCTACAATAGC+TGG 0.471925 tig0033038:-19025 None:intergenic
AAGACACTGCATGTATTAGT+TGG 0.476474 tig0033038:-19845 None:intergenic
GGATGACCAGCTTCAAATCC+AGG 0.477797 tig0033038:-17877 None:intergenic
TGCAAAACACCAAGGTTTGT+AGG 0.486752 tig0033038:-19728 None:intergenic
CCTTAGCCTTGTTCTTGTCA+GGG 0.490496 tig0033038:-17408 None:intergenic
GATTTATATGGAGATGAGAA+AGG 0.496080 tig0033038:+17850 Msa1450700:CDS
GATTCGAGAGTGAGAAAGCT+AGG 0.497255 tig0033038:+18989 Msa1450700:CDS
TCTTACAAGGAAAATGAAAA+GGG 0.501980 tig0033038:+18453 Msa1450700:CDS
ATTGTAGGTTGGCTACCTAA+TGG 0.504995 tig0033038:+19031 Msa1450700:CDS
TGGAAGCATTAACTGTGAAA+AGG 0.505775 tig0033038:+18368 Msa1450700:CDS
CGGCGGTGACTTGGCTGTCA+AGG 0.512307 tig0033038:+18491 Msa1450700:CDS
CACTTCCAGCTATTGTAGGT+TGG 0.512519 tig0033038:+19020 Msa1450700:CDS
TTATGGCCTTAAAACTCTTG+GGG 0.515568 tig0033038:-18116 None:intergenic
AAATGGAAGCGGCAGCTCTA+GGG 0.516656 tig0033038:+19447 Msa1450700:CDS
CATTGCCTTCAGTGATGTAC+AGG 0.517426 tig0033038:+18683 Msa1450700:CDS
TCTAGGGACTCCATTTCCTA+TGG 0.523006 tig0033038:+19463 Msa1450700:CDS
AAAGGAAGTCTCATTCTGTA+AGG 0.525693 tig0033038:+18386 Msa1450700:CDS
CGATCAATGTGGTGGCTGAA+CGG 0.527312 tig0033038:-16605 None:intergenic
AAGAAAGAAATAGTTGATGA+AGG 0.528006 tig0033038:+18153 Msa1450700:CDS
GTTGAGAGAAGCAGCACCCT+AGG 0.530951 tig0033038:-18606 None:intergenic
ATTTGGTTACCTACAAACCT+TGG 0.531218 tig0033038:+19719 Msa1450700:three_prime_UTR
TTGCCTACAAACCTCGGAAA+GGG 0.533121 tig0033038:+19566 Msa1450700:CDS
CAAAACAATCGACCCGTACA+AGG 0.536128 tig0033038:+16668 Msa1450700:CDS
GGAAACCCTGGATTTGAAGC+TGG 0.537781 tig0033038:+17871 Msa1450700:CDS
ATTCCTTGTTAATCATGAGA+TGG 0.539933 tig0033038:-19910 None:intergenic
GGTCTCAATCATCTCAATCT+GGG 0.540073 tig0033038:+18082 Msa1450700:CDS
TACAGGTGAAATGACAATAG+AGG 0.544357 tig0033038:+19603 Msa1450700:CDS
TCCTTGTCAAGAAAACCAAA+TGG 0.544854 tig0033038:+19430 Msa1450700:CDS
TATGGCCTTAAAACTCTTGG+GGG 0.545828 tig0033038:-18115 None:intergenic
CTATAATGGACTCAAAGTGT+TGG 0.546531 tig0033038:-18221 None:intergenic
GGATTTGAAGCTGGTCATCC+GGG 0.546824 tig0033038:+17880 Msa1450700:CDS
AAGGGTTCTCTGGTGGAGTA+CGG 0.550243 tig0033038:+18471 Msa1450700:CDS
TCTTATTCACCCTCTTTGAG+GGG 0.552771 tig0033038:+18195 Msa1450700:CDS
TAGAGGTGTATCTTATTGAA+CGG 0.553512 tig0033038:+19992 Msa1450700:three_prime_UTR
TATTGATTGCCTACAAACCT+CGG 0.555595 tig0033038:+19560 Msa1450700:CDS
AGAATGACTTAGAGCCTCCC+TGG 0.555880 tig0033038:-17960 None:intergenic
GTCTCATTCTGTAAGGAGCT+CGG 0.559611 tig0033038:+18393 Msa1450700:CDS
ACGGGTCATCATGACGGTGA+CGG 0.560421 tig0033038:-16586 None:intergenic
CCTTTAGATCTTCCAAAGCA+AGG 0.562071 tig0033038:+19278 Msa1450700:CDS
CTTTAGATCTTCCAAAGCAA+GGG 0.565533 tig0033038:+19279 Msa1450700:CDS
AAAAGAAAGTAACCTTGTAC+GGG 0.565722 tig0033038:-16680 None:intergenic
CTTTGAGTCCATTATAGGGG+AGG 0.569961 tig0033038:+18227 Msa1450700:CDS
GGAGGTTATGGTGGTCCTGG+TGG 0.572253 tig0033038:+17901 Msa1450700:CDS
TGTTGGATGCCCCTCAAAGA+GGG 0.575397 tig0033038:-18204 None:intergenic
TCAGACCAGGGGAACAACAA+TGG 0.575494 tig0033038:+17932 Msa1450700:CDS
AAGGAAACACAAGGCGGCGA+CGG 0.576209 tig0033038:-16631 None:intergenic
CACTGCTGAAAAGTTACCTA+GGG 0.576457 tig0033038:+18590 Msa1450700:CDS
GCACTGCTGAAAAGTTACCT+AGG 0.578112 tig0033038:+18589 Msa1450700:CDS
GGTGGATTTAACTTCAGACC+AGG 0.582161 tig0033038:+17919 Msa1450700:CDS
GGAGATGAGAAAGGAAACCC+TGG 0.582670 tig0033038:+17859 Msa1450700:CDS
CCCTGACAAGAACAAGGCTA+AGG 0.583292 tig0033038:+17408 Msa1450700:CDS
TCAAGAAAACCAAATGGAAG+CGG 0.585318 tig0033038:+19436 Msa1450700:CDS
ATCACCAGAGCCGCCGCCAA+AGG 0.586364 tig0033038:-17989 None:intergenic
AAACTTTGAGGCTCTTTGTG+GGG 0.588840 tig0033038:+19228 Msa1450700:CDS
TGAAGAATCTAAGAGACCAT+AGG 0.589085 tig0033038:-19479 None:intergenic
CCAGGACCACCATAACCTCC+CGG 0.595008 tig0033038:-17898 None:intergenic
ACAGTGAATTTCCCTTTCCG+AGG 0.599381 tig0033038:-19577 None:intergenic
GAGGTTGCTGGTACACTGCA+AGG 0.600210 tig0033038:+18246 Msa1450700:CDS
TGGTATATCTCCGCTTAGAA+CGG 0.601976 tig0033038:-19647 None:intergenic
ATTGCCTACAAACCTCGGAA+AGG 0.606305 tig0033038:+19565 Msa1450700:CDS
TGTTCGTGTAAATAAGTTCG+CGG 0.606599 tig0033038:-16494 None:intergenic
AAAAGCCTTGGAGCTTTGCG+CGG 0.611357 tig0033038:-18423 None:intergenic
GGTCATCCGGGAGGTTATGG+TGG 0.611379 tig0033038:+17892 Msa1450700:CDS
TATATCCGCGCAAAGCTCCA+AGG 0.612102 tig0033038:+18418 Msa1450700:CDS
AAATGAAAAGGGTTCTCTGG+TGG 0.612472 tig0033038:+18464 Msa1450700:CDS
GTTCGTGTAAATAAGTTCGC+GGG 0.614250 tig0033038:-16493 None:intergenic
GCATACCTGTACATCACTGA+AGG 0.621284 tig0033038:-18688 None:intergenic
ACACTTTGAGTCCATTATAG+GGG 0.621315 tig0033038:+18224 Msa1450700:CDS
CCTGACAAGAACAAGGCTAA+GGG 0.624057 tig0033038:+17409 Msa1450700:CDS
GATGACCAGCTTCAAATCCA+GGG 0.625923 tig0033038:-17876 None:intergenic
GAACAACAATGGGGTAACCA+GGG 0.633630 tig0033038:+17943 Msa1450700:CDS
GTGGATTTAACTTCAGACCA+GGG 0.636460 tig0033038:+17920 Msa1450700:CDS
ATTCTATCTGCAAAACACCA+AGG 0.636927 tig0033038:-19736 None:intergenic
GTGTTGGATGCCCCTCAAAG+AGG 0.638891 tig0033038:-18205 None:intergenic
GTAACGGTGACGGAATTGAT+CGG 0.642760 tig0033038:-16519 None:intergenic
AAAACTTATTCTGAAAACAG+GGG 0.643772 tig0033038:+19057 Msa1450700:CDS
GGACCATCTCATGATTAACA+AGG 0.644691 tig0033038:+19907 Msa1450700:three_prime_UTR
TTCGAAATCGAAACTGAGAA+CGG 0.645684 tig0033038:-16552 None:intergenic
GGTCTGAAGTTAAATCCACC+AGG 0.648334 tig0033038:-17916 None:intergenic
TTTATCAGCTCCGTTCTAAG+CGG 0.648770 tig0033038:+19637 Msa1450700:CDS
ATATCACCCTGACAAGAACA+AGG 0.649669 tig0033038:+17402 Msa1450700:CDS
GGCTGAACGGGTCATCATGA+CGG 0.650028 tig0033038:-16592 None:intergenic
CAACAATGGGGTAACCAGGG+AGG 0.659802 tig0033038:+17946 Msa1450700:CDS
GACGGTGACGATCAATGTGG+TGG 0.676126 tig0033038:-16613 None:intergenic
AAGTCAACGAGAAATTCAGA+AGG 0.680585 tig0033038:+17263 Msa1450700:CDS
TGGATTTAACTTCAGACCAG+GGG 0.681095 tig0033038:+17921 Msa1450700:CDS
TTTGAAGCTGGTCATCCGGG+AGG 0.700469 tig0033038:+17883 Msa1450700:CDS
GGTTCTCTGGTGGAGTACGG+CGG 0.703008 tig0033038:+18474 Msa1450700:CDS
TAGTTGATGAAGGAATGACT+TGG 0.720265 tig0033038:+18163 Msa1450700:CDS
AGACCAGGGGAACAACAATG+GGG 0.745284 tig0033038:+17934 Msa1450700:CDS
TTGTAAAAGGAAACACAAGG+CGG 0.763662 tig0033038:-16637 None:intergenic
GGATTGTAAAAGGAAACACA+AGG 0.768636 tig0033038:-16640 None:intergenic
GGCGACGGTGACGATCAATG+TGG 0.773922 tig0033038:-16616 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAACAATATATATATA+AGG - tig0033038:17364-17383 None:intergenic 10.0%
!! TGAGATTAAAATATGAAAAA+AGG + tig0033038:16919-16938 Msa1450700:intron 15.0%
!! AAGATTATAAACATATATGA+TGG - tig0033038:18837-18856 None:intergenic 15.0%
!!! AGTAATATTGTTTTAATTAG+AGG + tig0033038:19975-19994 Msa1450700:three_prime_UTR 15.0%
!!! TAGTTATTAGTTTATTGCAT+TGG + tig0033038:16828-16847 Msa1450700:intron 20.0%
!!! TGTTACTTTCTTCTTTAATT+TGG + tig0033038:16869-16888 Msa1450700:intron 20.0%
!! TGAAAAGAGTTTATAGAAAT+AGG - tig0033038:17558-17577 None:intergenic 20.0%
!! GTAACTTATTATACTACTAA+GGG - tig0033038:17714-17733 None:intergenic 20.0%
!! AGTAACTTATTATACTACTA+AGG - tig0033038:17715-17734 None:intergenic 20.0%
!!! TAGTATAATAAGTTACTTTC+TGG + tig0033038:17718-17737 Msa1450700:intron 20.0%
!! AGGAAGAATTATGATTTATA+TGG + tig0033038:17838-17857 Msa1450700:CDS 20.0%
!! CTTGTAGATATTTGAATTTA+TGG - tig0033038:18136-18155 None:intergenic 20.0%
!!! GTATATTTCAGATTGTTTTT+TGG + tig0033038:18880-18899 Msa1450700:intron 20.0%
!!! AAAAACTTATTCTGAAAACA+GGG + tig0033038:19056-19075 Msa1450700:CDS 20.0%
!!! TTTTGAGAAAGTAAGTAAAA+AGG + tig0033038:19135-19154 Msa1450700:CDS 20.0%
!!! GATTGAAGAGTATTAATTAT+AGG + tig0033038:19886-19905 Msa1450700:three_prime_UTR 20.0%
! AAAAAGAAAGTAACCTTGTA+CGG - tig0033038:16684-16703 None:intergenic 25.0%
! ATAATTCGAGGTTAAAATAG+CGG + tig0033038:16721-16740 Msa1450700:intron 25.0%
!!! TACTTTCTTCTTTAATTTGG+TGG + tig0033038:16872-16891 Msa1450700:intron 25.0%
!! TGAAAAAAGGATGAATTTTG+AGG + tig0033038:16932-16951 Msa1450700:intron 25.0%
!! GAAAAAAGGATGAATTTTGA+GGG + tig0033038:16933-16952 Msa1450700:intron 25.0%
!! ATAAGAATTGAAGTGTTTAG+AGG + tig0033038:16967-16986 Msa1450700:intron 25.0%
!! TAAGAATTGAAGTGTTTAGA+GGG + tig0033038:16968-16987 Msa1450700:intron 25.0%
! CATCTTGTTCATTTGAATTT+GGG - tig0033038:17141-17160 None:intergenic 25.0%
!! TTGCACATTACAATTTTATC+AGG + tig0033038:17218-17237 Msa1450700:intron 25.0%
!! TTACAATTTTATCAGGTTCT+TGG + tig0033038:17225-17244 Msa1450700:intron 25.0%
!! TACAATTTTATCAGGTTCTT+GGG + tig0033038:17226-17245 Msa1450700:intron 25.0%
!! AAGTTTGCTCAGATAAATAA+TGG + tig0033038:17442-17461 Msa1450700:CDS 25.0%
!! TTAAGTTGTTTATCCAAACA+GGG - tig0033038:17516-17535 None:intergenic 25.0%
!! TTTTATCTGATGAACAAAAG+AGG + tig0033038:17818-17837 Msa1450700:CDS 25.0%
!! GATTCAAATTCTTTTGGATT+TGG + tig0033038:18009-18028 Msa1450700:CDS 25.0%
!!! AAATTCTTTTGGATTTGGTT+TGG + tig0033038:18014-18033 Msa1450700:CDS 25.0%
!!! AAGAAAGAAATAGTTGATGA+AGG + tig0033038:18153-18172 Msa1450700:CDS 25.0%
!!! TCTATTGATTGTTTTGTAGT+TGG + tig0033038:18310-18329 Msa1450700:intron 25.0%
! TAAGAATAAACAAAAAGCCT+TGG - tig0033038:18438-18457 None:intergenic 25.0%
!!! GCTTTTTGTTTATTCTTACA+AGG + tig0033038:18440-18459 Msa1450700:CDS 25.0%
! TTCTTACAAGGAAAATGAAA+AGG + tig0033038:18452-18471 Msa1450700:CDS 25.0%
! TCTTACAAGGAAAATGAAAA+GGG + tig0033038:18453-18472 Msa1450700:CDS 25.0%
!! AACCTTTTACATAAAGAACT+AGG - tig0033038:18761-18780 None:intergenic 25.0%
!!! TATTTCAGATTGTTTTTTGG+TGG + tig0033038:18883-18902 Msa1450700:intron 25.0%
! GTGTATCTTGAATTATTTAC+AGG + tig0033038:18952-18971 Msa1450700:intron 25.0%
!! AGAATAAGTTTTTCTCCATT+AGG - tig0033038:19049-19068 None:intergenic 25.0%
!! GAAAAACTTATTCTGAAAAC+AGG + tig0033038:19055-19074 Msa1450700:CDS 25.0%
!! AAAACTTATTCTGAAAACAG+GGG + tig0033038:19057-19076 Msa1450700:CDS 25.0%
! TCTGTAAAAGATGTAAAATC+TGG + tig0033038:19082-19101 Msa1450700:CDS 25.0%
! TTTGTCAAATGCATTTAGAA+AGG - tig0033038:19515-19534 None:intergenic 25.0%
!! ATAGAGCATTAAGGTATATT+TGG + tig0033038:19702-19721 Msa1450700:exon 25.0%
! TTATCTGATCATCAGATAAT+TGG + tig0033038:19940-19959 Msa1450700:three_prime_UTR 25.0%
!! ATCATCAGATAATTGGTAAT+AGG + tig0033038:19947-19966 Msa1450700:three_prime_UTR 25.0%
! GTCATGAAATGATATGAAAT+AGG - tig0033038:20019-20038 None:intergenic 25.0%
! TTTCATATCATTTCATGACT+TGG + tig0033038:20020-20039 Msa1450700:three_prime_UTR 25.0%
AAAAGAAAGTAACCTTGTAC+GGG - tig0033038:16683-16702 None:intergenic 30.0%
! TGTGAAGCTTTTATAATTCG+AGG + tig0033038:16709-16728 Msa1450700:intron 30.0%
ATTGCATTGGATTGCATTAT+TGG + tig0033038:16841-16860 Msa1450700:intron 30.0%
TTGCATTGGATTGCATTATT+GGG + tig0033038:16842-16861 Msa1450700:intron 30.0%
!! TAATTTGGTGGAACTTGTTT+TGG + tig0033038:16884-16903 Msa1450700:intron 30.0%
CCATCTTGTTCATTTGAATT+TGG - tig0033038:17142-17161 None:intergenic 30.0%
CCAAATTCAAATGAACAAGA+TGG + tig0033038:17139-17158 Msa1450700:intron 30.0%
! CTACATAAAATTTCCCCTTT+TGG - tig0033038:17167-17186 None:intergenic 30.0%
! GGGAAATTTTATGTAGTGAT+TGG + tig0033038:17171-17190 Msa1450700:intron 30.0%
! ACAATTTTATCAGGTTCTTG+GGG + tig0033038:17227-17246 Msa1450700:intron 30.0%
! GTTAAGTTGTTTATCCAAAC+AGG - tig0033038:17517-17536 None:intergenic 30.0%
! ACTCTTTTCATAAGCTATCT+TGG + tig0033038:17568-17587 Msa1450700:intron 30.0%
!!! GTGTCATAAGCTGTTTTTAT+AGG + tig0033038:17623-17642 Msa1450700:intron 30.0%
! TAAGCATTGCAAGATTGTTA+GGG - tig0033038:17653-17672 None:intergenic 30.0%
! ATAAGCATTGCAAGATTGTT+AGG - tig0033038:17654-17673 None:intergenic 30.0%
ACTTATTTGAGCTTATCTAC+TGG - tig0033038:17678-17697 None:intergenic 30.0%
!! TCTACCTATTTCTTGTTGTT+AGG + tig0033038:17743-17762 Msa1450700:intron 30.0%
ACATCCTAACAACAAGAAAT+AGG - tig0033038:17750-17769 None:intergenic 30.0%
GATTTATATGGAGATGAGAA+AGG + tig0033038:17850-17869 Msa1450700:CDS 30.0%
! TCTGGTGATTCAAATTCTTT+TGG + tig0033038:18003-18022 Msa1450700:CDS 30.0%
!!! GATCTTCTTGGAAACTTTTT+TGG + tig0033038:18039-18058 Msa1450700:CDS 30.0%
!!! GGAAACTTTTTTGGTGATTT+TGG + tig0033038:18048-18067 Msa1450700:CDS 30.0%
! TTTATGGCCTTAAAACTCTT+GGG - tig0033038:18120-18139 None:intergenic 30.0%
! ATTTATGGCCTTAAAACTCT+TGG - tig0033038:18121-18140 None:intergenic 30.0%
AACACTTTGAGTCCATTATA+GGG + tig0033038:18223-18242 Msa1450700:CDS 30.0%
! ACTTGCCAATGTTTTCAAAA+CGG + tig0033038:18538-18557 Msa1450700:CDS 30.0%
!!! TCAATCCGTTTTGAAAACAT+TGG - tig0033038:18546-18565 None:intergenic 30.0%
!!! AATTACAGGAGTGTTCTTTT+TGG - tig0033038:18631-18650 None:intergenic 30.0%
AAAAGAACACTCCTGTAATT+TGG + tig0033038:18631-18650 Msa1450700:CDS 30.0%
! AGCCTAGTTCTTTATGTAAA+AGG + tig0033038:18756-18775 Msa1450700:intron 30.0%
TAGTTAATTCATTAGTCGCA+TGG + tig0033038:18790-18809 Msa1450700:intron 30.0%
!! TCGTTAAAGCATTAAGTTGA+TGG - tig0033038:19344-19363 None:intergenic 30.0%
TATCTCGTGCAAAATCTTAA+AGG + tig0033038:19378-19397 Msa1450700:intron 30.0%
ATCTCGTGCAAAATCTTAAA+GGG + tig0033038:19379-19398 Msa1450700:intron 30.0%
CTAAATGCATTTGACAAAAC+AGG + tig0033038:19517-19536 Msa1450700:CDS 30.0%
TTCTTATGTGTTTCCCTAAA+TGG - tig0033038:19670-19689 None:intergenic 30.0%
TTAATGCTCTATTTGAGTAC+AGG - tig0033038:19694-19713 None:intergenic 30.0%
! TGTACTCAAATAGAGCATTA+AGG + tig0033038:19693-19712 Msa1450700:exon 30.0%
TGTATCATATGTACTCTTGT+TGG - tig0033038:19778-19797 None:intergenic 30.0%
ATTCCTTGTTAATCATGAGA+TGG - tig0033038:19913-19932 None:intergenic 30.0%
! TAGAGGTGTATCTTATTGAA+CGG + tig0033038:19992-20011 Msa1450700:three_prime_UTR 30.0%
TGTTCGTGTAAATAAGTTCG+CGG - tig0033038:16497-16516 None:intergenic 35.0%
!! AACTGAGAACGGTGTTTTAA+CGG - tig0033038:16544-16563 None:intergenic 35.0%
TTCGAAATCGAAACTGAGAA+CGG - tig0033038:16555-16574 None:intergenic 35.0%
TTGTAAAAGGAAACACAAGG+CGG - tig0033038:16640-16659 None:intergenic 35.0%
GGATTGTAAAAGGAAACACA+AGG - tig0033038:16643-16662 None:intergenic 35.0%
!!! TTTTGGCTTCGGATTGTAAA+AGG - tig0033038:16653-16672 None:intergenic 35.0%
! AGCGGAAAGGTTAAATTTTG+AGG + tig0033038:16739-16758 Msa1450700:intron 35.0%
! GCGGAAAGGTTAAATTTTGA+GGG + tig0033038:16740-16759 Msa1450700:intron 35.0%
! CGGAAAGGTTAAATTTTGAG+GGG + tig0033038:16741-16760 Msa1450700:intron 35.0%
AAGTCAACGAGAAATTCAGA+AGG + tig0033038:17263-17282 Msa1450700:CDS 35.0%
TCAAATAAGTCAGTCCAAAC+AGG + tig0033038:17688-17707 Msa1450700:intron 35.0%
! AAGTCATTCTCCTTTTCCTT+TGG + tig0033038:17973-17992 Msa1450700:CDS 35.0%
!! TTTGGTTTGGATGATCTTCT+TGG + tig0033038:18027-18046 Msa1450700:CDS 35.0%
!! GTGATTTTGGCAGTTCATTT+AGG + tig0033038:18061-18080 Msa1450700:CDS 35.0%
! TTATGGCCTTAAAACTCTTG+GGG - tig0033038:18119-18138 None:intergenic 35.0%
!! TAGTTGATGAAGGAATGACT+TGG + tig0033038:18163-18182 Msa1450700:CDS 35.0%
TATCTTATTCACCCTCTTTG+AGG + tig0033038:18193-18212 Msa1450700:CDS 35.0%
ATCTTATTCACCCTCTTTGA+GGG + tig0033038:18194-18213 Msa1450700:CDS 35.0%
!! CTATAATGGACTCAAAGTGT+TGG - tig0033038:18224-18243 None:intergenic 35.0%
CAACACTTTGAGTCCATTAT+AGG + tig0033038:18222-18241 Msa1450700:CDS 35.0%
ACACTTTGAGTCCATTATAG+GGG + tig0033038:18224-18243 Msa1450700:CDS 35.0%
!! TGGAAGCATTAACTGTGAAA+AGG + tig0033038:18368-18387 Msa1450700:CDS 35.0%
AAAGGAAGTCTCATTCTGTA+AGG + tig0033038:18386-18405 Msa1450700:CDS 35.0%
ATTTCTTTCTCTGCACATGT+AGG + tig0033038:18730-18749 Msa1450700:intron 35.0%
!!! AGATTGTTTTTTGGTGGAAG+TGG + tig0033038:18889-18908 Msa1450700:intron 35.0%
! TGAATCAGCTTGTTCTTTCT+TGG - tig0033038:19176-19195 None:intergenic 35.0%
! AACACATTCAGTTGCTTTCT+CGG + tig0033038:19203-19222 Msa1450700:CDS 35.0%
CTTTAGATCTTCCAAAGCAA+GGG + tig0033038:19279-19298 Msa1450700:CDS 35.0%
TTCTGTGAGACCTGAAATAA+CGG - tig0033038:19409-19428 None:intergenic 35.0%
TCCTTGTCAAGAAAACCAAA+TGG + tig0033038:19430-19449 Msa1450700:CDS 35.0%
!!! TCCATTTGGTTTTCTTGACA+AGG - tig0033038:19434-19453 None:intergenic 35.0%
TCAAGAAAACCAAATGGAAG+CGG + tig0033038:19436-19455 Msa1450700:CDS 35.0%
ATCTAAGAGACCATAGGAAA+TGG - tig0033038:19476-19495 None:intergenic 35.0%
TGAAGAATCTAAGAGACCAT+AGG - tig0033038:19482-19501 None:intergenic 35.0%
! TATTGATTGCCTACAAACCT+CGG + tig0033038:19560-19579 Msa1450700:CDS 35.0%
GGGAAATTCACTGTATTTAC+AGG + tig0033038:19586-19605 Msa1450700:CDS 35.0%
TACAGGTGAAATGACAATAG+AGG + tig0033038:19603-19622 Msa1450700:CDS 35.0%
TAAGCGGAGATATACCATTT+AGG + tig0033038:19653-19672 Msa1450700:CDS 35.0%
AAGCGGAGATATACCATTTA+GGG + tig0033038:19654-19673 Msa1450700:CDS 35.0%
ATTTGGTTACCTACAAACCT+TGG + tig0033038:19719-19738 Msa1450700:three_prime_UTR 35.0%
ATTCTATCTGCAAAACACCA+AGG - tig0033038:19739-19758 None:intergenic 35.0%
AAGACACTGCATGTATTAGT+TGG - tig0033038:19848-19867 None:intergenic 35.0%
! AGAGGTGTATCTTATTGAAC+GGG + tig0033038:19993-20012 Msa1450700:three_prime_UTR 35.0%
GTTCGTGTAAATAAGTTCGC+GGG - tig0033038:16496-16515 None:intergenic 40.0%
!!! TGTTTTAACGGTAACGGTGA+CGG - tig0033038:16532-16551 None:intergenic 40.0%
!!! GAACGGTGTTTTAACGGTAA+CGG - tig0033038:16538-16557 None:intergenic 40.0%
! TCGAGGTTAAAATAGCGGAA+AGG + tig0033038:16726-16745 Msa1450700:intron 40.0%
ATGAACAAGATGGCTCCAAA+AGG + tig0033038:17149-17168 Msa1450700:intron 40.0%
TGAACAAGATGGCTCCAAAA+GGG + tig0033038:17150-17169 Msa1450700:intron 40.0%
ATATCACCCTGACAAGAACA+AGG + tig0033038:17402-17421 Msa1450700:CDS 40.0%
TGCCACTGGATAAAAGACTA+AGG + tig0033038:17490-17509 Msa1450700:intron 40.0%
TAAAAGACTAAGGCCCTGTT+TGG + tig0033038:17500-17519 Msa1450700:intron 40.0%
TATACTACTAAGGGCCTGTT+TGG - tig0033038:17705-17724 None:intergenic 40.0%
ATCTCGTACGCTGTTAAAAG+AGG - tig0033038:17799-17818 None:intergenic 40.0%
! GTGGATTTAACTTCAGACCA+GGG + tig0033038:17920-17939 Msa1450700:CDS 40.0%
! TGGATTTAACTTCAGACCAG+GGG + tig0033038:17921-17940 Msa1450700:CDS 40.0%
! TCATTCTCCTTTTCCTTTGG+CGG + tig0033038:17976-17995 Msa1450700:CDS 40.0%
AGGTCTCAATCATCTCAATC+TGG + tig0033038:18081-18100 Msa1450700:CDS 40.0%
GGTCTCAATCATCTCAATCT+GGG + tig0033038:18082-18101 Msa1450700:CDS 40.0%
! TATGGCCTTAAAACTCTTGG+GGG - tig0033038:18118-18137 None:intergenic 40.0%
TCTTATTCACCCTCTTTGAG+GGG + tig0033038:18195-18214 Msa1450700:CDS 40.0%
GGAAAATGAAAAGGGTTCTC+TGG + tig0033038:18461-18480 Msa1450700:CDS 40.0%
AAATGAAAAGGGTTCTCTGG+TGG + tig0033038:18464-18483 Msa1450700:CDS 40.0%
CACTGCTGAAAAGTTACCTA+GGG + tig0033038:18590-18609 Msa1450700:CDS 40.0%
CTAAGAACACGCCAAATTAC+AGG - tig0033038:18645-18664 None:intergenic 40.0%
GTAATTTGGCGTGTTCTTAG+TGG + tig0033038:18645-18664 Msa1450700:CDS 40.0%
ATGTACAGGTATGCAAGTGA+TGG + tig0033038:18697-18716 Msa1450700:intron 40.0%
! ATTGTAGGTTGGCTACCTAA+TGG + tig0033038:19031-19050 Msa1450700:CDS 40.0%
!! TCAAACTTTGAGGCTCTTTG+TGG + tig0033038:19226-19245 Msa1450700:CDS 40.0%
!! CAAACTTTGAGGCTCTTTGT+GGG + tig0033038:19227-19246 Msa1450700:CDS 40.0%
!! AAACTTTGAGGCTCTTTGTG+GGG + tig0033038:19228-19247 Msa1450700:CDS 40.0%
AGAACTCCAGTATGCGTAAT+TGG + tig0033038:19253-19272 Msa1450700:CDS 40.0%
!! CCTTGCTTTGGAAGATCTAA+AGG - tig0033038:19281-19300 None:intergenic 40.0%
CCTTTAGATCTTCCAAAGCA+AGG + tig0033038:19278-19297 Msa1450700:CDS 40.0%
!!! TTCTAGCTTTTCCCTTGCTT+TGG - tig0033038:19293-19312 None:intergenic 40.0%
GCTAGAATCACTTCTCACTT+TGG + tig0033038:19306-19325 Msa1450700:CDS 40.0%
! TTTATCAGCTCCGTTCTAAG+CGG + tig0033038:19637-19656 Msa1450700:CDS 40.0%
TGGTATATCTCCGCTTAGAA+CGG - tig0033038:19650-19669 None:intergenic 40.0%
TGCAAAACACCAAGGTTTGT+AGG - tig0033038:19731-19750 None:intergenic 40.0%
GGACCATCTCATGATTAACA+AGG + tig0033038:19907-19926 Msa1450700:three_prime_UTR 40.0%
!! GTAACGGTGACGGAATTGAT+CGG - tig0033038:16522-16541 None:intergenic 45.0%
! CTTGTACGGGTCGATTGTTT+TGG - tig0033038:16670-16689 None:intergenic 45.0%
CAAAACAATCGACCCGTACA+AGG + tig0033038:16668-16687 Msa1450700:CDS 45.0%
GAACAAGATGGCTCCAAAAG+GGG + tig0033038:17151-17170 Msa1450700:intron 45.0%
ACCTCGTCACATGTTAAACC+CGG + tig0033038:17320-17339 Msa1450700:intron 45.0%
CCAAGTTATCAGAGATAGCC+GGG - tig0033038:17341-17360 None:intergenic 45.0%
GCCAAGTTATCAGAGATAGC+CGG - tig0033038:17342-17361 None:intergenic 45.0%
!! CCTTAGCCTTGTTCTTGTCA+GGG - tig0033038:17411-17430 None:intergenic 45.0%
CCTGACAAGAACAAGGCTAA+GGG + tig0033038:17409-17428 Msa1450700:CDS 45.0%
GCTTTCTCTCTATATGCCAC+TGG + tig0033038:17476-17495 Msa1450700:intron 45.0%
!! GGCCTTAGTCTTTTATCCAG+TGG - tig0033038:17495-17514 None:intergenic 45.0%
GATGACCAGCTTCAAATCCA+GGG - tig0033038:17879-17898 None:intergenic 45.0%
! TGGATTTGAAGCTGGTCATC+CGG + tig0033038:17879-17898 Msa1450700:CDS 45.0%
! GGTCTGAAGTTAAATCCACC+AGG - tig0033038:17919-17938 None:intergenic 45.0%
! GGTGGATTTAACTTCAGACC+AGG + tig0033038:17919-17938 Msa1450700:CDS 45.0%
GAACAACAATGGGGTAACCA+GGG + tig0033038:17943-17962 Msa1450700:CDS 45.0%
AAAACTCTTGGGGGAACTCT+TGG - tig0033038:18109-18128 None:intergenic 45.0%
! GAGTTCCCCCAAGAGTTTTA+AGG + tig0033038:18110-18129 Msa1450700:CDS 45.0%
CTTTGAGTCCATTATAGGGG+AGG + tig0033038:18227-18246 Msa1450700:CDS 45.0%
! TACACTGCAAGGAGCTTTGA+AGG + tig0033038:18257-18276 Msa1450700:CDS 45.0%
GCTTCCAACCTGTCAAAAGA+GGG - tig0033038:18355-18374 None:intergenic 45.0%
TGCTTCCAACCTGTCAAAAG+AGG - tig0033038:18356-18375 None:intergenic 45.0%
GTCTCATTCTGTAAGGAGCT+CGG + tig0033038:18393-18412 Msa1450700:CDS 45.0%
GCACTGCTGAAAAGTTACCT+AGG + tig0033038:18589-18608 Msa1450700:CDS 45.0%
CATTGCCTTCAGTGATGTAC+AGG + tig0033038:18683-18702 Msa1450700:CDS 45.0%
GCATACCTGTACATCACTGA+AGG - tig0033038:18691-18710 None:intergenic 45.0%
GATTCGAGAGTGAGAAAGCT+AGG + tig0033038:18989-19008 Msa1450700:CDS 45.0%
GATGCACTTCCAGCTATTGT+AGG + tig0033038:19016-19035 Msa1450700:CDS 45.0%
CACTTCCAGCTATTGTAGGT+TGG + tig0033038:19020-19039 Msa1450700:CDS 45.0%
GCTTTCTCGGTCAAACTTTG+AGG + tig0033038:19216-19235 Msa1450700:CDS 45.0%
! AAGGCACCAATTACGCATAC+TGG - tig0033038:19262-19281 None:intergenic 45.0%
!! AAAGGGAGCACCGTTATTTC+AGG + tig0033038:19396-19415 Msa1450700:intron 45.0%
TCTAGGGACTCCATTTCCTA+TGG + tig0033038:19463-19482 Msa1450700:CDS 45.0%
ATTGCCTACAAACCTCGGAA+AGG + tig0033038:19565-19584 Msa1450700:CDS 45.0%
TTGCCTACAAACCTCGGAAA+GGG + tig0033038:19566-19585 Msa1450700:CDS 45.0%
TTTCCCTTTCCGAGGTTTGT+AGG - tig0033038:19572-19591 None:intergenic 45.0%
ACAGTGAATTTCCCTTTCCG+AGG - tig0033038:19580-19599 None:intergenic 45.0%
!!! ATTTTAATTGAAATATTTTT+AGG + tig0033038:16776-16795 Msa1450700:intron 5.0%
!!! TTTTAATTGAAATATTTTTA+GGG + tig0033038:16777-16796 Msa1450700:intron 5.0%
GATCAATGTGGTGGCTGAAC+GGG - tig0033038:16607-16626 None:intergenic 50.0%
CGATCAATGTGGTGGCTGAA+CGG - tig0033038:16608-16627 None:intergenic 50.0%
!!! CGGGTCGATTGTTTTGGCTT+CGG - tig0033038:16664-16683 None:intergenic 50.0%
CCCGGCTATCTCTGATAACT+TGG + tig0033038:17338-17357 Msa1450700:intron 50.0%
CCCTGACAAGAACAAGGCTA+AGG + tig0033038:17408-17427 Msa1450700:CDS 50.0%
!! CCCTTAGCCTTGTTCTTGTC+AGG - tig0033038:17412-17431 None:intergenic 50.0%
GGAGATGAGAAAGGAAACCC+TGG + tig0033038:17859-17878 Msa1450700:CDS 50.0%
! GGAAACCCTGGATTTGAAGC+TGG + tig0033038:17871-17890 Msa1450700:CDS 50.0%
GGATGACCAGCTTCAAATCC+AGG - tig0033038:17880-17899 None:intergenic 50.0%
! GGATTTGAAGCTGGTCATCC+GGG + tig0033038:17880-17899 Msa1450700:CDS 50.0%
TCAGACCAGGGGAACAACAA+TGG + tig0033038:17932-17951 Msa1450700:CDS 50.0%
CAGACCAGGGGAACAACAAT+GGG + tig0033038:17933-17952 Msa1450700:CDS 50.0%
AGACCAGGGGAACAACAATG+GGG + tig0033038:17934-17953 Msa1450700:CDS 50.0%
! TTACCCCATTGTTGTTCCCC+TGG - tig0033038:17940-17959 None:intergenic 50.0%
GGAACAACAATGGGGTAACC+AGG + tig0033038:17942-17961 Msa1450700:CDS 50.0%
AGAATGACTTAGAGCCTCCC+TGG - tig0033038:17963-17982 None:intergenic 50.0%
! TTCTCCTTTTCCTTTGGCGG+CGG + tig0033038:17979-17998 Msa1450700:CDS 50.0%
TGTTGGATGCCCCTCAAAGA+GGG - tig0033038:18207-18226 None:intergenic 50.0%
TCCATTATAGGGGAGGTTGC+TGG + tig0033038:18234-18253 Msa1450700:CDS 50.0%
ACCAGCAACCTCCCCTATAA+TGG - tig0033038:18238-18257 None:intergenic 50.0%
! GTCTCTCTCCCTCTTTTGAC+AGG + tig0033038:18344-18363 Msa1450700:intron 50.0%
! CTCTCCCTCTTTTGACAGGT+TGG + tig0033038:18348-18367 Msa1450700:intron 50.0%
TATATCCGCGCAAAGCTCCA+AGG + tig0033038:18418-18437 Msa1450700:CDS 50.0%
AAAAGCCTTGGAGCTTTGCG+CGG - tig0033038:18426-18445 None:intergenic 50.0%
AAGGGTTCTCTGGTGGAGTA+CGG + tig0033038:18471-18490 Msa1450700:CDS 50.0%
GGTAGCCAACCTACAATAGC+TGG - tig0033038:19028-19047 None:intergenic 50.0%
AAATGGAAGCGGCAGCTCTA+GGG + tig0033038:19447-19466 Msa1450700:CDS 50.0%
ACGGGTCATCATGACGGTGA+CGG - tig0033038:16589-16608 None:intergenic 55.0%
GGCTGAACGGGTCATCATGA+CGG - tig0033038:16595-16614 None:intergenic 55.0%
GACGGTGACGATCAATGTGG+TGG - tig0033038:16616-16635 None:intergenic 55.0%
AAGGAAACACAAGGCGGCGA+CGG - tig0033038:16634-16653 None:intergenic 55.0%
GCCGGGTTTAACATGTGACG+AGG - tig0033038:17324-17343 None:intergenic 55.0%
! TTTGAAGCTGGTCATCCGGG+AGG + tig0033038:17883-17902 Msa1450700:CDS 55.0%
CAACAATGGGGTAACCAGGG+AGG + tig0033038:17946-17965 Msa1450700:CDS 55.0%
GTGTTGGATGCCCCTCAAAG+AGG - tig0033038:18208-18227 None:intergenic 55.0%
GAGGTTGCTGGTACACTGCA+AGG + tig0033038:18246-18265 Msa1450700:CDS 55.0%
GTTGAGAGAAGCAGCACCCT+AGG - tig0033038:18609-18628 None:intergenic 55.0%
! CTAGAGCTGCCGCTTCCATT+TGG - tig0033038:19448-19467 None:intergenic 55.0%
CAAATGGAAGCGGCAGCTCT+AGG + tig0033038:19446-19465 Msa1450700:CDS 55.0%
GGCGACGGTGACGATCAATG+TGG - tig0033038:16619-16638 None:intergenic 60.0%
GCTGGTCATCCGGGAGGTTA+TGG + tig0033038:17889-17908 Msa1450700:CDS 60.0%
GGTCATCCGGGAGGTTATGG+TGG + tig0033038:17892-17911 Msa1450700:CDS 60.0%
CCAGGACCACCATAACCTCC+CGG - tig0033038:17901-17920 None:intergenic 60.0%
GGAGGTTATGGTGGTCCTGG+TGG + tig0033038:17901-17920 Msa1450700:CDS 60.0%
AGAGCCGCCGCCAAAGGAAA+AGG - tig0033038:17986-18005 None:intergenic 60.0%
! TTTTCCTTTGGCGGCGGCTC+TGG + tig0033038:17985-18004 Msa1450700:CDS 60.0%
GGTTCTCTGGTGGAGTACGG+CGG + tig0033038:18474-18493 Msa1450700:CDS 60.0%
CCGGGAGGTTATGGTGGTCC+TGG + tig0033038:17898-17917 Msa1450700:CDS 65.0%
ATCACCAGAGCCGCCGCCAA+AGG - tig0033038:17992-18011 None:intergenic 65.0%
GGTGGAGTACGGCGGTGACT+TGG + tig0033038:18482-18501 Msa1450700:CDS 65.0%
! CGGCGGTGACTTGGCTGTCA+AGG + tig0033038:18491-18510 Msa1450700:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0033038 gene 16493 20111 16493 ID=Msa1450700;Name=Msa1450700
tig0033038 mRNA 16493 20111 16493 ID=Msa1450700-mRNA-1;Parent=Msa1450700;Name=Msa1450700-mRNA-1;_AED=0.04;_eAED=0.04;_QI=104|1|1|1|1|1|7|406|557
tig0033038 exon 16493 16689 16493 ID=Msa1450700-mRNA-1:exon:17594;Parent=Msa1450700-mRNA-1
tig0033038 exon 17240 17295 17240 ID=Msa1450700-mRNA-1:exon:17595;Parent=Msa1450700-mRNA-1
tig0033038 exon 17390 17463 17390 ID=Msa1450700-mRNA-1:exon:17596;Parent=Msa1450700-mRNA-1
tig0033038 exon 17809 18278 17809 ID=Msa1450700-mRNA-1:exon:17597;Parent=Msa1450700-mRNA-1
tig0033038 exon 18366 18704 18366 ID=Msa1450700-mRNA-1:exon:17598;Parent=Msa1450700-mRNA-1
tig0033038 exon 18974 19327 18974 ID=Msa1450700-mRNA-1:exon:17599;Parent=Msa1450700-mRNA-1
tig0033038 exon 19418 20111 19418 ID=Msa1450700-mRNA-1:exon:17600;Parent=Msa1450700-mRNA-1
tig0033038 five_prime_UTR 16493 16596 16493 ID=Msa1450700-mRNA-1:five_prime_utr;Parent=Msa1450700-mRNA-1
tig0033038 CDS 16597 16689 16597 ID=Msa1450700-mRNA-1:cds;Parent=Msa1450700-mRNA-1
tig0033038 CDS 17240 17295 17240 ID=Msa1450700-mRNA-1:cds;Parent=Msa1450700-mRNA-1
tig0033038 CDS 17390 17463 17390 ID=Msa1450700-mRNA-1:cds;Parent=Msa1450700-mRNA-1
tig0033038 CDS 17809 18278 17809 ID=Msa1450700-mRNA-1:cds;Parent=Msa1450700-mRNA-1
tig0033038 CDS 18366 18704 18366 ID=Msa1450700-mRNA-1:cds;Parent=Msa1450700-mRNA-1
tig0033038 CDS 18974 19327 18974 ID=Msa1450700-mRNA-1:cds;Parent=Msa1450700-mRNA-1
tig0033038 CDS 19418 19705 19418 ID=Msa1450700-mRNA-1:cds;Parent=Msa1450700-mRNA-1
tig0033038 three_prime_UTR 19706 20111 19706 ID=Msa1450700-mRNA-1:three_prime_utr;Parent=Msa1450700-mRNA-1
Gene Sequence

>Msa1450700

ATGATGACCCGTTCAGCCACCACATTGATCGTCACCGTCGCCGCCTTGTGTTTCCTTTTACAATCCGAAGCCAAAACAATCGACCCGTACAAGGTTCTTGGGGTTGATAAAAGTGCAAGTCAACGAGAAATTCAGAAGGCTTTTCACAAACTCTCTCTTCAATATCACCCTGACAAGAACAAGGCTAAGGGTGCACAAGAGAAGTTTGCTCAGATAAATAATGCGTACGAGATTTTATCTGATGAACAAAAGAGGAAGAATTATGATTTATATGGAGATGAGAAAGGAAACCCTGGATTTGAAGCTGGTCATCCGGGAGGTTATGGTGGTCCTGGTGGATTTAACTTCAGACCAGGGGAACAACAATGGGGTAACCAGGGAGGCTCTAAGTCATTCTCCTTTTCCTTTGGCGGCGGCTCTGGTGATTCAAATTCTTTTGGATTTGGTTTGGATGATCTTCTTGGAAACTTTTTTGGTGATTTTGGCAGTTCATTTAGGTCTCAATCATCTCAATCTGGGTCCAAGAGTTCCCCCAAGAGTTTTAAGGCCATAAATTCAAATATCTACAAGAAAGAAATAGTTGATGAAGGAATGACTTGGCTTTTGTTATCTTATTCACCCTCTTTGAGGGGCATCCAACACTTTGAGTCCATTATAGGGGAGGTTGCTGGTACACTGCAAGGAGCTTTGAAGGTTGGAAGCATTAACTGTGAAAAGGAAGTCTCATTCTGTAAGGAGCTCGGTGTATATCCGCGCAAAGCTCCAAGGCTTTTTGTTTATTCTTACAAGGAAAATGAAAAGGGTTCTCTGGTGGAGTACGGCGGTGACTTGGCTGTCAAGGATTTGAAAGCTTTCTGTCAACAACACTTGCCAATGTTTTCAAAACGGATTGACTTGAATCAACTTGATCAATTAAGCACTGCTGAAAAGTTACCTAGGGTGCTGCTTCTCTCAACCAAAAAGAACACTCCTGTAATTTGGCGTGTTCTTAGTGGCTTGTATCGCAAACGCATTGCCTTCAGTGATGTACAGGTTCATGATGATGCTGATTCGAGAGTGAGAAAGCTAGGAGTTGATGCACTTCCAGCTATTGTAGGTTGGCTACCTAATGGAGAAAAACTTATTCTGAAAACAGGGGTTTCTGTAAAAGATGTAAAATCTGGTGTACAAGATCTTAGTAAGATACTTGATAGTTTTGAGAAAGTAAGTAAAAAGGAAACATCAAGTCAGTCCAAGAAAGAACAAGCTGATTCAGAAGACAAACACATTCAGTTGCTTTCTCGGTCAAACTTTGAGGCTCTTTGTGGGGAGAGAACTCCAGTATGCGTAATTGGTGCCTTTAGATCTTCCAAAGCAAGGGAAAAGCTAGAATCACTTCTCACTTTGGTCTCACAGAAGTCCTTGTCAAGAAAACCAAATGGAAGCGGCAGCTCTAGGGACTCCATTTCCTATGGTCTCTTAGATTCTTCAAAGCAACAATCCTTTCTAAATGCATTTGACAAAACAGGTTATAAATCATCAGATAAGTTATTGATTGCCTACAAACCTCGGAAAGGGAAATTCACTGTATTTACAGGTGAAATGACAATAGAGGAAGTAGAGAATTTTATCAGCTCCGTTCTAAGCGGAGATATACCATTTAGGGAAACACATAAGAAGCCTGTACTCAAATAG
Protein sequence

>Msa1450700

MMTRSATTLIVTVAALCFLLQSEAKTIDPYKVLGVDKSASQREIQKAFHKLSLQYHPDKNKAKGAQEKFAQINNAYEILSDEQKRKNYDLYGDEKGNPGFEAGHPGGYGGPGGFNFRPGEQQWGNQGGSKSFSFSFGGGSGDSNSFGFGLDDLLGNFFGDFGSSFRSQSSQSGSKSSPKSFKAINSNIYKKEIVDEGMTWLLLSYSPSLRGIQHFESIIGEVAGTLQGALKVGSINCEKEVSFCKELGVYPRKAPRLFVYSYKENEKGSLVEYGGDLAVKDLKAFCQQHLPMFSKRIDLNQLDQLSTAEKLPRVLLLSTKKNTPVIWRVLSGLYRKRIAFSDVQVHDDADSRVRKLGVDALPAIVGWLPNGEKLILKTGVSVKDVKSGVQDLSKILDSFEKVSKKETSSQSKKEQADSEDKHIQLLSRSNFEALCGERTPVCVIGAFRSSKAREKLESLLTLVSQKSLSRKPNGSGSSRDSISYGLLDSSKQQSFLNAFDKTGYKSSDKLLIAYKPRKGKFTVFTGEMTIEEVENFISSVLSGDIPFRETHKKPVLK*