AlfalfaGEDB | Msa1458630

M. sativa cultivar zhongmu-4 / Msa1458630

KEGG: K16292
GO: GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005788 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0030312 GO:0031974 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0070013 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575
PFAM: PF00112.26

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1458630	XP_013460973.1	96.591	88	3	0	1	88	275	362	1.53e-54	182

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1458630	sp\|P12412\|CYSEP_VIGMU	85.227	88	13	0	1	88	275	362	1.22e-49	162

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1458630	G8A2F7	96.591	88	3	0	1	88	275	362	7.28e-55	182

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1435360	Msa1458630	0.807585	4.704646e-50	-8.615850e-47
Msa0418930	Msa1458630	0.802615	5.158243e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1458630	MtrunA17_Chr3g0116551	96.591	88	3	0	1	88	275	362	1.40e-58	182
Msa1458630	MtrunA17_Chr4g0058081	76.136	88	20	1	1	88	274	360	6.64e-43	142
Msa1458630	MtrunA17_Chr7g0248661	64.286	70	25	0	1	70	7	76	2.84e-31	104
Msa1458630	MtrunA17_Chr4g0024411	71.014	69	20	0	2	70	274	342	2.87e-31	111
Msa1458630	MtrunA17_Chr4g0041061	72.059	68	19	0	2	69	276	343	3.99e-31	111
Msa1458630	MtrunA17_Chr4g0040881	71.014	69	20	0	2	70	276	344	4.67e-31	111
Msa1458630	MtrunA17_Chr4g0060251	70.588	68	20	0	2	69	276	343	9.65e-31	110
Msa1458630	MtrunA17_Chr5g0405751	69.565	69	21	0	2	70	275	343	1.32e-30	110
Msa1458630	MtrunA17_Chr4g0041081	68.116	69	22	0	2	70	276	344	2.62e-30	109
Msa1458630	MtrunA17_Chr4g0041521	67.647	68	21	1	2	69	272	338	3.50e-30	108
Msa1458630	MtrunA17_Chr7g0248371	60.870	69	27	0	2	70	63	131	4.17e-30	104
Msa1458630	MtrunA17_Chr7g0248811	66.176	68	23	0	2	69	267	334	8.48e-30	107
Msa1458630	MtrunA17_Chr4g0041481	67.647	68	21	1	2	69	272	338	1.06e-29	107
Msa1458630	MtrunA17_Chr7g0248391	59.722	72	29	0	2	73	269	340	2.23e-29	106
Msa1458630	MtrunA17_Chr4g0040851	66.197	71	21	2	1	70	291	359	1.13e-28	105
Msa1458630	MtrunA17_Chr1g0208261	70.423	71	19	2	2	71	327	396	1.16e-28	106
Msa1458630	MtrunA17_Chr2g0325121	59.722	72	28	1	1	72	268	338	1.34e-27	102
Msa1458630	MtrunA17_Chr1g0153011	68.056	72	21	2	2	72	274	344	1.53e-27	103
Msa1458630	MtrunA17_Chr4g0041471	59.420	69	28	0	2	70	273	341	9.65e-27	99.8
Msa1458630	MtrunA17_Chr2g0319991	57.971	69	29	0	2	70	275	343	7.80e-26	97.4
Msa1458630	MtrunA17_Chr3g0144281	61.429	70	26	1	2	71	280	348	2.32e-25	96.3
Msa1458630	MtrunA17_Chr4g0053311	61.972	71	25	2	2	71	273	342	3.77e-25	95.9
Msa1458630	MtrunA17_Chr3g0142071	58.904	73	29	1	1	73	287	358	5.75e-25	96.3
Msa1458630	MtrunA17_Chr3g0134231	60.563	71	26	2	2	71	293	362	1.22e-24	95.5
Msa1458630	MtrunA17_Chr2g0315491	70.588	68	20	0	2	69	272	339	2.59e-24	93.6
Msa1458630	MtrunA17_Chr2g0283671	51.471	68	33	0	3	70	160	227	3.13e-22	85.9
Msa1458630	MtrunA17_Chr2g0283661	50.000	68	34	0	2	69	203	270	8.19e-21	83.2
Msa1458630	MtrunA17_Chr1g0156161	49.254	67	33	1	2	68	196	261	9.62e-17	72.0
Msa1458630	MtrunA17_Chr2g0283901	47.297	74	33	3	2	71	285	356	1.82e-16	72.4
Msa1458630	MtrunA17_Chr2g0283741	45.570	79	40	2	9	87	298	373	2.27e-16	72.0
Msa1458630	MtrunA17_Chr0c27g0493921	44.595	74	32	4	1	70	280	348	6.30e-14	65.1
Msa1458630	MtrunA17_Chr2g0311381	40.541	74	37	4	2	69	277	349	1.88e-12	60.8
Msa1458630	MtrunA17_Chr8g0379151	47.368	76	33	4	2	71	299	373	1.99e-12	60.8
Msa1458630	MtrunA17_Chr2g0311471	44.000	75	35	4	2	70	273	346	5.19e-11	57.0
Msa1458630	MtrunA17_Chr2g0311391	43.103	58	28	1	11	68	316	368	6.85e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1458630	AT5G50260.1	75.281	89	21	1	1	88	273	361	4.54e-44	145
Msa1458630	AT3G48340.1	65.909	88	29	1	1	88	275	361	3.38e-34	120
Msa1458630	AT3G48350.1	62.500	88	32	1	1	88	274	360	4.55e-33	117
Msa1458630	AT4G11320.2	65.278	72	24	1	2	73	292	362	1.47e-27	102
Msa1458630	AT4G11320.1	65.278	72	24	1	2	73	292	362	1.47e-27	102
Msa1458630	AT4G11310.1	63.889	72	25	1	2	73	285	355	1.48e-27	102
Msa1458630	AT5G45890.1	57.353	68	29	0	2	69	277	344	3.98e-27	101
Msa1458630	AT1G47128.1	61.972	71	26	1	2	72	285	354	1.09e-26	101
Msa1458630	AT4G36880.1	63.636	77	25	3	2	77	293	367	1.13e-26	100
Msa1458630	AT5G43060.1	67.143	70	22	1	2	71	286	354	1.46e-26	101
Msa1458630	AT4G23520.1	56.757	74	31	1	3	76	283	355	6.75e-26	98.2
Msa1458630	AT3G19390.1	58.667	75	30	1	2	76	278	351	2.41e-25	97.8
Msa1458630	AT3G19400.1	54.321	81	36	1	2	82	281	360	3.71e-25	96.3
Msa1458630	AT2G27420.1	55.072	69	31	0	2	70	279	347	5.42e-25	95.5
Msa1458630	AT1G06260.1	64.286	70	24	1	2	71	275	343	1.31e-24	94.7
Msa1458630	AT4G35350.1	61.429	70	26	1	2	71	285	353	3.07e-24	93.6
Msa1458630	AT2G34080.1	56.522	69	30	0	2	70	276	344	6.10e-24	92.8
Msa1458630	AT3G49340.1	52.174	69	33	0	2	70	272	340	1.14e-23	92.0
Msa1458630	AT1G20850.1	60.000	70	27	1	2	71	286	354	2.07e-23	91.7
Msa1458630	AT1G09850.1	55.714	70	30	1	2	71	266	334	2.33e-23	92.0
Msa1458630	AT1G29110.1	57.143	70	28	2	2	70	265	333	9.57e-23	89.4
Msa1458630	AT1G29080.1	54.688	64	29	0	7	70	282	345	1.34e-21	86.7
Msa1458630	AT1G29090.1	53.968	63	29	0	8	70	292	354	1.38e-20	84.0
Msa1458630	AT3G43960.1	49.383	81	39	2	2	80	277	357	3.79e-20	82.8
Msa1458630	AT5G60360.1	47.945	73	29	4	2	70	289	356	7.52e-15	68.2
Msa1458630	AT3G45310.1	44.595	74	32	4	1	70	288	356	7.28e-14	65.5
Msa1458630	AT5G60360.2	46.575	73	29	5	2	70	289	355	1.43e-12	61.6
Msa1458630	AT3G45310.2	43.243	74	32	5	1	70	288	355	1.39e-11	58.9

Find 0 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CCAATAATTAGTTCCATCAA+CGG	-	tig0039684:77-96	None:intergenic	30.0%
	GAACTAATTATTGGATTGTG+AGG	+	tig0039684:83-102	Msa1458630:CDS	30.0%
!!	TTTTGGAGGAGTTTTTAACT+GGG	-	tig0039684:210-229	None:intergenic	30.0%
!!	TTTTTGGAGGAGTTTTTAAC+TGG	-	tig0039684:211-230	None:intergenic	30.0%
	TGTGGTAAAGAGCTAAATCA+TGG	+	tig0039684:25-44	Msa1458630:CDS	35.0%
	AATCATGGTGTAGCAATAGT+AGG	+	tig0039684:40-59	Msa1458630:CDS	35.0%
!	CCGTTGATGGAACTAATTAT+TGG	+	tig0039684:74-93	Msa1458630:CDS	35.0%
	AAGGTTACATCAGAATGCAA+AGG	+	tig0039684:134-153	Msa1458630:CDS	35.0%
	GCAAAGGAATGTATCTAACA+AGG	+	tig0039684:150-169	Msa1458630:CDS	35.0%
	AGGAATGTATCTAACAAGGA+AGG	+	tig0039684:154-173	Msa1458630:CDS	35.0%
	AAAAACTCCTCCAAAAATCC+AGG	+	tig0039684:214-233	Msa1458630:CDS	35.0%
	TCATCCTTGGTAGATGATAA+AGG	-	tig0039684:244-263	None:intergenic	35.0%
	GGTGTAGCAATAGTAGGATA+TGG	+	tig0039684:46-65	Msa1458630:CDS	40.0%
	ATTGGATTGTGAGGAACTCA+TGG	+	tig0039684:92-111	Msa1458630:CDS	40.0%
	TTGGATTGTGAGGAACTCAT+GGG	+	tig0039684:93-112	Msa1458630:CDS	40.0%
	TCTAACAAGGAAGGACTTTG+TGG	+	tig0039684:163-182	Msa1458630:CDS	40.0%
!	TAAAGGTCCTCCTGGATTTT+TGG	-	tig0039684:227-246	None:intergenic	40.0%
	AGGACCTTTATCATCTACCA+AGG	+	tig0039684:237-256	Msa1458630:CDS	40.0%
!	GGAGTATTTACTGGCGATTG+TGG	+	tig0039684:7-26	Msa1458630:CDS	45.0%
	GGATATGGAACAACCGTTGA+TGG	+	tig0039684:61-80	Msa1458630:CDS	45.0%
	TGGATTGTGAGGAACTCATG+GGG	+	tig0039684:94-113	Msa1458630:CDS	45.0%
	AGGACTTTGTGGCATAGCTA+TGG	+	tig0039684:174-193	Msa1458630:CDS	45.0%
!!	GGAGGAGTTTTTAACTGGGT+AGG	-	tig0039684:206-225	None:intergenic	45.0%
	AACTCCTCCAAAAATCCAGG+AGG	+	tig0039684:217-236	Msa1458630:CDS	45.0%
	GTAGATGATAAAGGTCCTCC+TGG	-	tig0039684:235-254	None:intergenic	45.0%
!!	AGGTCCTCCTGGATTTTTGG+AGG	-	tig0039684:224-243	None:intergenic	50.0%
	TGTGAGGAACTCATGGGGAG+CGG	+	tig0039684:99-118	Msa1458630:CDS	55.0%
	GAACTCATGGGGAGCGGAAT+GGG	+	tig0039684:105-124	Msa1458630:CDS	55.0%
	AACTCATGGGGAGCGGAATG+GGG	+	tig0039684:106-125	Msa1458630:CDS	55.0%
	GGAACTCATGGGGAGCGGAA+TGG	+	tig0039684:104-123	Msa1458630:CDS	60.0%
	GGAGCGGAATGGGGTGAACA+AGG	+	tig0039684:115-134	Msa1458630:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0039684	gene	4	270	4	ID=Msa1458630;Name=Msa1458630
tig0039684	mRNA	4	270	4	ID=Msa1458630-mRNA-1;Parent=Msa1458630;Name=Msa1458630-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|88
tig0039684	exon	4	270	4	ID=Msa1458630-mRNA-1:exon:21861;Parent=Msa1458630-mRNA-1
tig0039684	CDS	4	270	4	ID=Msa1458630-mRNA-1:cds;Parent=Msa1458630-mRNA-1

Gene Sequence	>Msa1458630 CAGGGAGTATTTACTGGCGATTGTGGTAAAGAGCTAAATCATGGTGTAGCAATAGTAGGATATGGAACAACCGTTGATGGAACTAATTATTGGATTGTGAGGAACTCATGGGGAGCGGAATGGGGTGAACAAGGTTACATCAGAATGCAAAGGAATGTATCTAACAAGGAAGGACTTTGTGGCATAGCTATGGAAGCTTCCTACCCAGTTAAAAACTCCTCCAAAAATCCAGGAGGACCTTTATCATCTACCAAGGATGAGCTTTGA
Protein sequence	>Msa1458630 QGVFTGDCGKELNHGVAIVGYGTTVDGTNYWIVRNSWGAEWGEQGYIRMQRNVSNKEGLCGIAMEASYPVKNSSKNPGGPLSSTKDEL*