AlfalfaGEDB | Msa1435360

M. sativa cultivar zhongmu-4 / Msa1435360

KEGG: K16292
GO: GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005788 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0030312 GO:0031974 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0070013 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575
PFAM: PF08246.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1435360	XP_013460973.1	96.685	362	12	0	1	362	1	362	0.0	739

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1435360	sp\|P25803\|CYSEP_PHAVU	82.320	362	64	0	1	362	1	362	0.0	634

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1435360	G8A2F7	96.685	362	12	0	1	362	1	362	0.0	739

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0024400	Msa1435360	0.810074	1.381172e-50	-8.615850e-47
Msa0089980	Msa1435360	0.814133	1.799012e-51	-8.615850e-47
Msa0090010	Msa1435360	0.810040	1.404674e-50	-8.615850e-47
Msa0105410	Msa1435360	0.801709	7.921876e-49	-8.615850e-47
Msa1435360	Msa1458630	0.807585	4.704646e-50	-8.615850e-47
Msa1435360	Msa1461370	0.842946	1.895101e-58	-8.615850e-47
Msa0626270	Msa1435360	0.824260	8.886395e-54	-8.615850e-47
Msa0626460	Msa1435360	0.804918	1.714935e-49	-8.615850e-47
Msa0651560	Msa1435360	0.803526	3.342537e-49	-8.615850e-47
Msa0664540	Msa1435360	0.831701	1.437591e-55	-8.615850e-47
Msa0693330	Msa1435360	0.803549	3.305612e-49	-8.615850e-47
Msa0698100	Msa1435360	0.807263	5.505586e-50	-8.615850e-47
Msa0702720	Msa1435360	0.841108	5.803495e-58	-8.615850e-47
Msa0709270	Msa1435360	0.800165	1.637761e-48	-8.615850e-47
Msa0795360	Msa1435360	0.811653	6.285228e-51	-8.615850e-47
Msa0829330	Msa1435360	0.805315	1.416439e-49	-8.615850e-47
Msa0141400	Msa1435360	0.814099	1.830383e-51	-8.615850e-47
Msa0163510	Msa1435360	0.806998	6.263593e-50	-8.615850e-47
Msa0191560	Msa1435360	0.800819	1.205237e-48	-8.615850e-47
Msa0196860	Msa1435360	0.827909	1.205642e-54	-8.615850e-47
Msa0217670	Msa1435360	0.815467	9.106094e-52	-8.615850e-47
Msa0225100	Msa1435360	0.811440	6.994015e-51	-8.615850e-47
Msa0226310	Msa1435360	0.803486	3.407492e-49	-8.615850e-47
Msa1226460	Msa1435360	0.801595	8.361977e-49	-8.615850e-47
Msa1338370	Msa1435360	0.808462	3.060227e-50	-8.615850e-47
Msa1340200	Msa1435360	0.817965	2.508121e-52	-8.615850e-47
Msa1341840	Msa1435360	0.840444	8.664391e-58	-8.615850e-47
Msa0370770	Msa1435360	0.825145	5.497925e-54	-8.615850e-47
Msa0385830	Msa1435360	0.806741	7.098391e-50	-8.615850e-47
Msa0418930	Msa1435360	0.901638	2.188924e-78	-8.615850e-47
Msa0427700	Msa1435360	0.807136	5.857984e-50	-8.615850e-47
Msa0464810	Msa1435360	0.964324	3.488571e-123	-8.615850e-47
Msa0992510	Msa1435360	0.819487	1.132155e-52	-8.615850e-47
Msa0240900	Msa1435360	0.800662	1.297217e-48	-8.615850e-47
Msa0263920	Msa1435360	0.822087	2.857444e-53	-8.615850e-47
Msa0276400	Msa1435360	0.817298	3.545601e-52	-8.615850e-47
Msa0287740	Msa1435360	0.802164	6.387662e-49	-8.615850e-47
Msa0304570	Msa1435360	0.809968	1.455595e-50	-8.615850e-47
Msa0312220	Msa1435360	0.808124	3.613620e-50	-8.615850e-47
Msa0316440	Msa1435360	0.819518	1.113579e-52	-8.615850e-47
Msa0322900	Msa1435360	0.821489	3.930456e-53	-8.615850e-47
Msa0362890	Msa1435360	0.800175	1.630529e-48	-8.615850e-47
Msa1117910	Msa1435360	0.813876	2.049907e-51	-8.615850e-47
Msa1132470	Msa1435360	0.806866	6.679567e-50	-8.615850e-47
Msa1161810	Msa1435360	0.803575	3.265026e-49	-8.615850e-47
Msa0527190	Msa1435360	0.801853	7.401749e-49	-8.615850e-47
Msa0855870	Msa1435360	0.821017	5.047798e-53	-8.615850e-47
Msa0869280	Msa1435360	0.808303	3.309245e-50	-8.615850e-47
Msa0933760	Msa1435360	0.829921	3.927437e-55	-8.615850e-47
Msa0971650	Msa1435360	0.803903	2.791532e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1435360	MtrunA17_Chr3g0116551	96.685	362	12	0	1	362	1	362	0.0	739
Msa1435360	MtrunA17_Chr4g0058081	76.519	362	83	2	1	362	1	360	0.0	594
Msa1435360	MtrunA17_Chr4g0041481	58.013	312	120	7	35	343	35	338	1.49e-125	364
Msa1435360	MtrunA17_Chr4g0041521	57.372	312	122	7	35	343	35	338	5.70e-125	363
Msa1435360	MtrunA17_Chr4g0060251	55.205	317	126	5	35	343	35	343	4.81e-124	360
Msa1435360	MtrunA17_Chr5g0405751	55.172	319	129	6	33	344	32	343	1.24e-123	359
Msa1435360	MtrunA17_Chr4g0040881	54.829	321	136	6	28	344	29	344	2.52e-123	358
Msa1435360	MtrunA17_Chr4g0041061	52.011	348	157	7	1	343	1	343	2.55e-123	358
Msa1435360	MtrunA17_Chr4g0024411	57.643	314	122	7	38	344	33	342	2.63e-123	358
Msa1435360	MtrunA17_Chr3g0144281	51.576	349	158	5	3	345	5	348	1.50e-122	357
Msa1435360	MtrunA17_Chr1g0208261	55.521	326	140	5	23	345	73	396	2.31e-122	362
Msa1435360	MtrunA17_Chr4g0053311	51.613	341	150	5	8	345	14	342	3.17e-122	357
Msa1435360	MtrunA17_Chr4g0041081	52.923	325	136	6	28	344	29	344	2.00e-121	354
Msa1435360	MtrunA17_Chr3g0134231	52.273	352	155	8	6	345	12	362	9.35e-121	357
Msa1435360	MtrunA17_Chr2g0315491	53.779	344	144	7	5	343	6	339	1.22e-118	347
Msa1435360	MtrunA17_Chr4g0040851	50.877	342	156	6	6	344	27	359	1.97e-117	344
Msa1435360	MtrunA17_Chr2g0325121	49.713	348	163	5	1	346	1	338	5.54e-115	337
Msa1435360	MtrunA17_Chr4g0041471	51.792	307	144	3	40	344	37	341	1.06e-112	332
Msa1435360	MtrunA17_Chr1g0153011	50.430	349	145	8	6	346	16	344	1.13e-110	330
Msa1435360	MtrunA17_Chr2g0319991	46.903	339	169	5	10	344	12	343	2.63e-106	315
Msa1435360	MtrunA17_Chr7g0248391	46.789	327	157	7	25	347	27	340	4.15e-100	300
Msa1435360	MtrunA17_Chr7g0248811	45.665	346	173	7	1	343	1	334	4.49e-99	297
Msa1435360	MtrunA17_Chr3g0142071	49.196	311	155	3	39	347	49	358	1.70e-98	299
Msa1435360	MtrunA17_Chr2g0283901	44.582	323	161	11	32	345	43	356	3.12e-83	261
Msa1435360	MtrunA17_Chr8g0379151	43.099	355	176	13	6	345	30	373	1.97e-75	238
Msa1435360	MtrunA17_Chr2g0283741	40.116	344	186	12	27	361	41	373	2.09e-73	232
Msa1435360	MtrunA17_Chr0c27g0493921	43.624	298	148	9	53	344	65	348	4.78e-72	228
Msa1435360	MtrunA17_Chr2g0283671	51.852	216	101	3	129	344	15	227	4.88e-69	216
Msa1435360	MtrunA17_Chr4g0070441	43.554	287	144	9	53	331	65	341	3.27e-68	218
Msa1435360	MtrunA17_Chr2g0311381	40.303	330	155	16	40	343	36	349	2.63e-63	205
Msa1435360	MtrunA17_Chr1g0155781	40.134	299	151	9	54	336	66	352	2.40e-62	203
Msa1435360	MtrunA17_Chr2g0311471	41.528	301	159	11	55	344	52	346	1.60e-61	201
Msa1435360	MtrunA17_Chr4g0035551	37.793	299	157	8	54	336	63	348	1.06e-58	194
Msa1435360	MtrunA17_Chr2g0311391	38.192	343	162	13	40	342	36	368	5.23e-58	192
Msa1435360	MtrunA17_Chr1g0156161	42.731	227	122	6	119	342	40	261	9.75e-57	186
Msa1435360	MtrunA17_Chr1g0199131	31.672	341	198	10	15	336	34	358	3.28e-52	177
Msa1435360	MtrunA17_Chr7g0248421	52.866	157	69	3	152	307	57	209	1.38e-44	152
Msa1435360	MtrunA17_Chr2g0283661	31.429	315	144	8	32	343	25	270	7.89e-44	152
Msa1435360	MtrunA17_Chr4g0036731	34.615	286	148	7	54	336	63	312	5.82e-43	152
Msa1435360	MtrunA17_Chr7g0248371	63.636	99	35	1	246	344	34	131	2.06e-42	145
Msa1435360	MtrunA17_Chr2g0283751	36.082	291	150	13	6	274	10	286	1.85e-41	147
Msa1435360	MtrunA17_Chr4g0036281	33.677	291	148	9	54	336	63	316	2.49e-40	145
Msa1435360	MtrunA17_Chr4g0037291	33.677	291	148	9	54	336	104	357	4.26e-40	145
Msa1435360	MtrunA17_Chr7g0248661	64.000	75	27	0	270	344	2	76	5.94e-32	115
Msa1435360	MtrunA17_Chr2g0311091	39.259	135	71	2	55	178	50	184	1.10e-24	99.4
Msa1435360	MtrunA17_Chr1g0156771	46.535	101	50	3	127	224	54	153	7.88e-24	96.3
Msa1435360	MtrunA17_Chr7g0271041	26.812	276	166	11	86	336	66	330	2.31e-23	99.8
Msa1435360	MtrunA17_Chr7g0271031	29.918	244	139	10	130	350	153	387	2.27e-22	97.4
Msa1435360	MtrunA17_Chr8g0379671	37.692	130	70	5	169	296	23	143	1.49e-15	73.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1435360	AT5G50260.1	70.556	360	105	1	4	362	2	361	0.0	557
Msa1435360	AT3G48340.1	67.313	361	115	2	4	362	2	361	0.0	517
Msa1435360	AT3G48350.1	64.444	360	126	2	4	362	2	360	1.63e-177	497
Msa1435360	AT3G48350.2	65.942	276	92	2	5	278	2	277	1.37e-134	386
Msa1435360	AT1G20850.1	54.037	322	144	3	25	345	36	354	1.36e-121	355
Msa1435360	AT4G35350.1	51.003	349	161	4	3	345	9	353	4.74e-121	353
Msa1435360	AT5G43060.1	50.857	350	156	5	6	345	11	354	2.16e-118	351
Msa1435360	AT1G47128.1	49.568	347	164	4	6	346	13	354	2.16e-118	351
Msa1435360	AT3G19390.1	48.442	353	173	6	1	350	5	351	7.22e-116	344
Msa1435360	AT4G36880.1	51.368	329	149	6	32	351	41	367	4.57e-114	337
Msa1435360	AT5G45890.1	47.143	350	167	7	6	343	1	344	5.00e-114	335
Msa1435360	AT3G19400.1	51.911	314	141	7	39	347	43	351	5.10e-108	321
Msa1435360	AT1G06260.1	48.286	350	165	7	1	345	5	343	1.79e-106	316
Msa1435360	AT4G11310.1	46.797	359	170	8	3	347	4	355	1.01e-105	315
Msa1435360	AT4G11320.2	46.499	357	169	8	6	347	13	362	1.58e-103	310
Msa1435360	AT4G11320.1	46.499	357	169	8	6	347	13	362	1.58e-103	310
Msa1435360	AT1G09850.1	49.677	310	149	4	38	345	30	334	4.57e-100	303
Msa1435360	AT4G23520.1	46.835	316	158	6	39	350	46	355	5.69e-99	297
Msa1435360	AT2G27420.1	45.533	347	174	5	6	344	8	347	7.34e-99	297
Msa1435360	AT3G49340.1	44.118	340	183	5	9	344	4	340	1.81e-97	293
Msa1435360	AT2G34080.1	44.348	345	181	6	6	344	5	344	5.00e-94	285
Msa1435360	AT1G29090.1	44.722	360	168	11	1	344	10	354	6.20e-91	277
Msa1435360	AT3G43960.1	46.462	325	158	11	39	354	40	357	4.80e-89	273
Msa1435360	AT1G29080.1	41.311	351	193	8	1	344	1	345	1.35e-87	268
Msa1435360	AT4G35350.2	48.582	282	135	4	3	278	9	286	1.43e-86	263
Msa1435360	AT1G29110.1	39.031	351	189	9	1	344	1	333	2.88e-78	244
Msa1435360	AT3G19400.2	50.833	240	109	6	39	273	43	278	5.80e-73	229
Msa1435360	AT5G60360.1	41.333	300	156	9	51	344	71	356	8.81e-69	220
Msa1435360	AT3G45310.1	40.728	302	155	10	51	344	71	356	1.83e-66	214
Msa1435360	AT5G60360.2	41.000	300	156	10	51	344	71	355	3.14e-66	213
Msa1435360	AT3G45310.2	40.397	302	155	11	51	344	71	355	7.19e-64	207
Msa1435360	AT5G60360.3	40.206	291	154	9	51	335	71	347	1.31e-63	207
Msa1435360	AT2G21430.1	33.618	351	199	10	6	336	13	349	4.04e-60	198
Msa1435360	AT4G39090.1	36.789	299	161	8	54	336	66	352	1.07e-56	189
Msa1435360	AT3G54940.2	37.458	299	161	9	54	336	66	354	1.18e-54	184
Msa1435360	AT4G16190.1	37.248	298	161	9	55	336	71	358	6.44e-53	179
Msa1435360	AT3G54940.3	37.209	215	123	4	54	260	66	276	1.77e-38	139
Msa1435360	AT1G02305.1	27.596	337	209	11	50	362	33	358	1.91e-27	111
Msa1435360	AT4G01610.1	27.356	329	196	15	50	350	30	343	3.92e-23	99.4
Msa1435360	AT4G01610.2	26.748	329	198	15	50	350	30	343	2.89e-21	94.0
Msa1435360	AT2G22160.1	46.465	99	49	1	53	151	8	102	1.00e-20	86.7
Msa1435360	AT1G02300.1	31.381	239	132	10	147	362	146	375	1.24e-20	92.4

Find 115 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAGCCTATTTGTCTTTATT+TGG	0.139485	tig0027699:+55949	None:intergenic
CCGTTGATGGAACTAATTAT+TGG	0.225728	tig0027699:-55300	Msa1435360:CDS
TTTGATGACAACGTATGATT+TGG	0.286950	tig0027699:-54997	Msa1435360:three_prime_UTR
ATCAGATCCTCCGGCATCTA+TGG	0.300046	tig0027699:+55566	None:intergenic
AGTTTCCATGTTTCTTTGTT+TGG	0.301765	tig0027699:+56780	None:intergenic
TTGGAAGCAATTCACTTGTT+TGG	0.302311	tig0027699:+56799	None:intergenic
ACATGACCAACCATGAATTT+AGG	0.309976	tig0027699:-56501	Msa1435360:CDS
TTTGAGTTCATCAAGCAAAA+AGG	0.324145	tig0027699:-55846	Msa1435360:CDS
AAGCTCCTGCTTCTGTTGAT+TGG	0.324173	tig0027699:-56387	Msa1435360:CDS
GAACTCATGGGGAGCGGAAT+GGG	0.328427	tig0027699:-55269	Msa1435360:CDS
ATGTTTGTGTTGTTCAAGTA+TGG	0.330691	tig0027699:+56980	None:intergenic
CCGAGGACACATCTTTATCT+TGG	0.334739	tig0027699:+56692	None:intergenic
AATGATGAAGATGATAAAAT+AGG	0.342555	tig0027699:+56955	None:intergenic
TAAACCAAATAAAGACAAAT+AGG	0.344680	tig0027699:-55953	Msa1435360:CDS
TGTCCTGTATAGGTAGTTGC+TGG	0.345293	tig0027699:-56007	Msa1435360:intron
TCAAATATTTAAAGTTCATA+AGG	0.354352	tig0027699:+55028	None:intergenic
TGAATGGAAGTTGGATGAAA+AGG	0.372453	tig0027699:+56833	None:intergenic
GCATCTATGGCTACGGATAC+AGG	0.385812	tig0027699:+55579	None:intergenic
TCAACTGTTGTAGCTGTTGA+AGG	0.388321	tig0027699:-55978	Msa1435360:CDS
TTGTTAAGTTTCAACTTGTA+AGG	0.389898	tig0027699:+56532	None:intergenic
AGGAGGTTAAAGGTTTCTTA+AGG	0.390380	tig0027699:+56917	None:intergenic
AGGATGCAATGGTGGGCTAA+TGG	0.392108	tig0027699:-55877	Msa1435360:CDS
GATCCATCATTTGCTGTGTA+AGG	0.397020	tig0027699:+55798	None:intergenic
GCAGGGGATGGTTTATATAG+AGG	0.401101	tig0027699:+56862	None:intergenic
CACTTCCACGTGGCGTGCCT+CGG	0.411657	tig0027699:+56437	None:intergenic
GATTGTTCTGTCCATTGCTT+TGG	0.412192	tig0027699:-56743	Msa1435360:CDS
AACATTCATTCAAATATCAA+TGG	0.413261	tig0027699:-54869	Msa1435360:three_prime_UTR
GGAGGTTAAAGGTTTCTTAA+GGG	0.416407	tig0027699:+56918	None:intergenic
ATCAACAGAAGCAGGAGCTT+TGG	0.424537	tig0027699:+56390	None:intergenic
TGTATTAATTGAATGGAAGT+TGG	0.428819	tig0027699:+56824	None:intergenic
TGACCATCAATTGACACAGC+TGG	0.433204	tig0027699:+55657	None:intergenic
GAATGGAAGTTGGATGAAAA+GGG	0.443264	tig0027699:+56834	None:intergenic
GGTGTAGCAATAGTAGGATA+TGG	0.443941	tig0027699:-55328	Msa1435360:CDS
TGATGGTCATGAGACTGTTC+CGG	0.449890	tig0027699:-55643	Msa1435360:CDS
GGAGTCACCACACGGTTTCT+CGG	0.450148	tig0027699:-56636	Msa1435360:CDS
TTTGGAATGTATTAATTGAA+TGG	0.454640	tig0027699:+56817	None:intergenic
GGATATGGAACAACCGTTGA+TGG	0.454887	tig0027699:-55313	Msa1435360:CDS
GTCCCAGCTGTGTCAATTGA+TGG	0.458384	tig0027699:-55660	Msa1435360:CDS
CGAGGCACGCCACGTGGAAG+TGG	0.460548	tig0027699:-56436	Msa1435360:CDS
TCATCCTTGGTAGATGATAA+AGG	0.462977	tig0027699:+55133	None:intergenic
TTTCTCCAATCAACAGAAGC+AGG	0.466214	tig0027699:+56382	None:intergenic
TATCCATCTTGTTAGTGTTG+TGG	0.468486	tig0027699:+56557	None:intergenic
CATAGGTAGTCCTAAATTCA+TGG	0.476124	tig0027699:+56491	None:intergenic
CATAATGGTTGCAGGGAATG+AGG	0.484219	tig0027699:+54898	None:intergenic
TGGATGAAAAGGGAAAGAGC+AGG	0.491370	tig0027699:+56844	None:intergenic
ATTGGATTGTGAGGAACTCA+TGG	0.493389	tig0027699:-55282	Msa1435360:CDS
CTATTACAGGGAGTATTTAG+TGG	0.493552	tig0027699:-55376	Msa1435360:intron
AATCATGGTGTAGCAATAGT+AGG	0.495226	tig0027699:-55334	Msa1435360:CDS
GTCACTGAGGTCAAGGATCA+AGG	0.495703	tig0027699:-56349	Msa1435360:CDS
TCTAACAAGGAAGGACTTTG+TGG	0.498260	tig0027699:-55211	Msa1435360:CDS
TTATATAGAGGATTGAAGAA+AGG	0.504717	tig0027699:+56874	None:intergenic
GAGGTTAAAGGTTTCTTAAG+GGG	0.504781	tig0027699:+56919	None:intergenic
GTAGATGATAAAGGTCCTGC+TGG	0.507201	tig0027699:+55142	None:intergenic
AAACATTCAAAGCTCATCCT+TGG	0.508057	tig0027699:+55120	None:intergenic
GGATGAAAAGGGAAAGAGCA+GGG	0.510179	tig0027699:+56845	None:intergenic
TACCCTTACACAGCAAATGA+TGG	0.512248	tig0027699:-55801	Msa1435360:CDS
GAATTTAGGACTACCTATGC+TGG	0.513702	tig0027699:-56487	Msa1435360:CDS
GGAACTCATGGGGAGCGGAA+TGG	0.524402	tig0027699:-55270	Msa1435360:CDS
GTCCTCGGACAAAAGCTTGT+GGG	0.525849	tig0027699:-56677	Msa1435360:CDS
AGGACTTTGTGGCATAGCTA+TGG	0.526821	tig0027699:-55200	Msa1435360:CDS
TGTGACAATCAAGAAAATCA+AGG	0.527505	tig0027699:-55897	Msa1435360:CDS
GAAAATCAAGGATGCAATGG+TGG	0.535112	tig0027699:-55885	Msa1435360:CDS
GTCCTGTATAGGTAGTTGCT+GGG	0.537057	tig0027699:-56006	Msa1435360:intron
TCCTCCGGCATCTATGGCTA+CGG	0.543132	tig0027699:+55572	None:intergenic
GACTACCTATGCTGGATCAA+AGG	0.546319	tig0027699:-56479	Msa1435360:CDS
CAAGAAAATCAAGGATGCAA+TGG	0.548537	tig0027699:-55888	Msa1435360:CDS
AAACTGGAAATCAGATCCTC+CGG	0.552551	tig0027699:+55557	None:intergenic
TCTGTTGATTGGAGAAAGAA+AGG	0.553167	tig0027699:-56376	Msa1435360:CDS
GGCTACGGATACAGGTTGGT+TGG	0.553342	tig0027699:+55587	None:intergenic
TTTAAGACATAATGGTTGCA+GGG	0.557691	tig0027699:+54891	None:intergenic
AGGTGCTGTCACTGAGGTCA+AGG	0.558185	tig0027699:-56356	Msa1435360:CDS
ATAATGGTTGCAGGGAATGA+GGG	0.560027	tig0027699:+54899	None:intergenic
ATCCATCATTTGCTGTGTAA+GGG	0.560080	tig0027699:+55799	None:intergenic
TGTCCACAACACTAACAAGA+TGG	0.563716	tig0027699:-56560	Msa1435360:CDS
TGTGGTAAAGAGCTAAATCA+TGG	0.564153	tig0027699:-55349	Msa1435360:CDS
CCAAGATAAAGATGTGTCCT+CGG	0.565632	tig0027699:-56692	Msa1435360:CDS
GACCATCAATTGACACAGCT+GGG	0.568817	tig0027699:+55658	None:intergenic
TTGTTCAAGTATGGAAGCAA+AGG	0.571573	tig0027699:+56989	None:intergenic
CCAATAATTAGTTCCATCAA+CGG	0.573198	tig0027699:+55300	None:intergenic
TGTGGGACTTATACGAGAGA+TGG	0.573304	tig0027699:-56660	Msa1435360:CDS
TTGGATTGTGAGGAACTCAT+GGG	0.574375	tig0027699:-55281	Msa1435360:CDS
AACTCATGGGGAGCGGAATG+GGG	0.577276	tig0027699:-55268	Msa1435360:CDS
AAAGCATCTTCATCGTTAGC+CGG	0.584931	tig0027699:+55624	None:intergenic
AAATTCATGGTTGGTCATGT+CGG	0.587621	tig0027699:+56504	None:intergenic
GGAGCGGAATGGGGTGAACA+AGG	0.588520	tig0027699:-55259	Msa1435360:CDS
ATTAACCTTTGATCCAGCAT+AGG	0.590030	tig0027699:+56474	None:intergenic
ATCTTGGAAATCGAAGCTCT+CGG	0.596119	tig0027699:+56708	None:intergenic
GGAGTATTTAGTGGCGATTG+TGG	0.600793	tig0027699:-55367	Msa1435360:CDS
TACTTCAAGAACCAAAGCAA+TGG	0.600914	tig0027699:+56732	None:intergenic
TGTGAGGAACTCATGGGGAG+CGG	0.601438	tig0027699:-55275	Msa1435360:CDS
AAAAGGGAAAGAGCAGGGGA+TGG	0.601628	tig0027699:+56850	None:intergenic
TGTCCTCGGACAAAAGCTTG+TGG	0.602219	tig0027699:-56678	Msa1435360:CDS
ATGTTCCGAGGCACGCCACG+TGG	0.610728	tig0027699:-56442	Msa1435360:CDS
GCGTGCCTCGGAACATTCTG+TGG	0.611727	tig0027699:+56449	None:intergenic
AGGAATGTATCTAACAAGGA+AGG	0.612463	tig0027699:-55220	Msa1435360:CDS
GAACTAATTATTGGATTGTG+AGG	0.613342	tig0027699:-55291	Msa1435360:CDS
GGTAGTCCTAAATTCATGGT+TGG	0.613532	tig0027699:+56495	None:intergenic
TGCTTCCAAACAAAGAAACA+TGG	0.616974	tig0027699:-56785	Msa1435360:exon
AAAATCAAGGATGCAATGGT+GGG	0.617377	tig0027699:-55884	Msa1435360:CDS
ATAAGCTCTTGTTCAGACAA+AGG	0.634536	tig0027699:+55924	None:intergenic
AAGGTTACATCAGAATGCAA+AGG	0.641547	tig0027699:-55240	Msa1435360:CDS
GCAAAGGAATGTATCTAACA+AGG	0.642601	tig0027699:-55224	Msa1435360:CDS
CTATGGCTACGGATACAGGT+TGG	0.643007	tig0027699:+55583	None:intergenic
GATGAAAAGGGAAAGAGCAG+GGG	0.645380	tig0027699:+56846	None:intergenic
GGGACTTATACGAGAGATGG+AGG	0.652822	tig0027699:-56657	Msa1435360:CDS
GTATCCGTAGCCATAGATGC+CGG	0.655334	tig0027699:-55576	Msa1435360:CDS
AGGACCTTTATCATCTACCA+AGG	0.670444	tig0027699:-55137	Msa1435360:CDS
AATCACCACAGAATGTTCCG+AGG	0.693791	tig0027699:-56454	Msa1435360:CDS
TCCGTAGCCATAGATGCCGG+AGG	0.697215	tig0027699:-55573	Msa1435360:CDS
GTCAAGGATCAAGGCCAATG+TGG	0.699707	tig0027699:-56340	Msa1435360:intron
GAGATGGAGGAGTCACCACA+CGG	0.702988	tig0027699:-56644	Msa1435360:CDS
TGGATTGTGAGGAACTCATG+GGG	0.709984	tig0027699:-55280	Msa1435360:CDS
CAAGGTTCCGAGAAACCGTG+TGG	0.715039	tig0027699:+56629	None:intergenic
AAAGAAAGGTGCTGTCACTG+AGG	0.722701	tig0027699:-56362	Msa1435360:CDS
ATGAATGTTCCACTTCCACG+TGG	0.756623	tig0027699:+56427	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAAAATATATGATCT+TGG	+	tig0027699:55773-55792	None:intergenic	10.0%
!!!	TTTTTACTTTTTTATTTTGA+AGG	+	tig0027699:56385-56404	None:intergenic	10.0%
!!	AAAAAAAAAATATATGATCT+TGG	+	tig0027699:55773-55792	None:intergenic	10.0%
!!!	TTTTTACTTTTTTATTTTGA+AGG	+	tig0027699:56385-56404	None:intergenic	10.0%
!!!	ATGAATAATTTTTGAATCAT+AGG	+	tig0027699:56160-56179	None:intergenic	15.0%
!!	TAAAAAAGTAAAAAAAGAGA+TGG	-	tig0027699:56391-56410	Msa1435360:CDS	15.0%
!!!	TCAAATATTTAAAGTTCATA+AGG	+	tig0027699:56828-56847	None:intergenic	15.0%
!!!	ATGAATAATTTTTGAATCAT+AGG	+	tig0027699:56160-56179	None:intergenic	15.0%
!!	TAAAAAAGTAAAAAAAGAGA+TGG	-	tig0027699:56391-56410	Msa1435360:CDS	15.0%
!!!	TCAAATATTTAAAGTTCATA+AGG	+	tig0027699:56828-56847	None:intergenic	15.0%
!!	AATGATGAAGATGATAAAAT+AGG	+	tig0027699:54901-54920	None:intergenic	20.0%
!!	AAACTCAATAAAAAAATGTG+AGG	+	tig0027699:54959-54978	None:intergenic	20.0%
!!!	TTTGGAATGTATTAATTGAA+TGG	+	tig0027699:55039-55058	None:intergenic	20.0%
!!	TAAACCAAATAAAGACAAAT+AGG	-	tig0027699:55900-55919	Msa1435360:CDS	20.0%
!!!	ATGAATGTTTTAAGACATAA+TGG	+	tig0027699:56973-56992	None:intergenic	20.0%
!!	AACATTCATTCAAATATCAA+TGG	-	tig0027699:56984-57003	Msa1435360:five_prime_UTR	20.0%
!!	AATGATGAAGATGATAAAAT+AGG	+	tig0027699:54901-54920	None:intergenic	20.0%
!!	AAACTCAATAAAAAAATGTG+AGG	+	tig0027699:54959-54978	None:intergenic	20.0%
!!!	TTTGGAATGTATTAATTGAA+TGG	+	tig0027699:55039-55058	None:intergenic	20.0%
!!	TAAACCAAATAAAGACAAAT+AGG	-	tig0027699:55900-55919	Msa1435360:CDS	20.0%
!!!	ATGAATGTTTTAAGACATAA+TGG	+	tig0027699:56973-56992	None:intergenic	20.0%
!!	AACATTCATTCAAATATCAA+TGG	-	tig0027699:56984-57003	Msa1435360:five_prime_UTR	20.0%
!	TTATATAGAGGATTGAAGAA+AGG	+	tig0027699:54982-55001	None:intergenic	25.0%
!	TGTATTAATTGAATGGAAGT+TGG	+	tig0027699:55032-55051	None:intergenic	25.0%
!	TTGTTAAGTTTCAACTTGTA+AGG	+	tig0027699:55324-55343	None:intergenic	25.0%
!!!	ACTTTTTTAACTAACCACAT+TGG	+	tig0027699:55530-55549	None:intergenic	25.0%
!	TATAATTGACATGCAACTTT+AGG	-	tig0027699:56132-56151	Msa1435360:intron	25.0%
!	TCAAAAATTATTCATGTTGC+AGG	-	tig0027699:56165-56184	Msa1435360:intron	25.0%
!!	ATTTTGTATACACCTATTAC+AGG	-	tig0027699:56464-56483	Msa1435360:CDS	25.0%
!!	TTTTGTATACACCTATTACA+GGG	-	tig0027699:56465-56484	Msa1435360:CDS	25.0%
!	TTATATAGAGGATTGAAGAA+AGG	+	tig0027699:54982-55001	None:intergenic	25.0%
!	TGTATTAATTGAATGGAAGT+TGG	+	tig0027699:55032-55051	None:intergenic	25.0%
!	TTGTTAAGTTTCAACTTGTA+AGG	+	tig0027699:55324-55343	None:intergenic	25.0%
!!!	ACTTTTTTAACTAACCACAT+TGG	+	tig0027699:55530-55549	None:intergenic	25.0%
!	TATAATTGACATGCAACTTT+AGG	-	tig0027699:56132-56151	Msa1435360:intron	25.0%
!	TCAAAAATTATTCATGTTGC+AGG	-	tig0027699:56165-56184	Msa1435360:intron	25.0%
!!	ATTTTGTATACACCTATTAC+AGG	-	tig0027699:56464-56483	Msa1435360:CDS	25.0%
!!	TTTTGTATACACCTATTACA+GGG	-	tig0027699:56465-56484	Msa1435360:CDS	25.0%
!	ATGTTTGTGTTGTTCAAGTA+TGG	+	tig0027699:54876-54895	None:intergenic	30.0%
	AAAAAATGTGAGGAGGTTAA+AGG	+	tig0027699:54949-54968	None:intergenic	30.0%
	CTCAATAAAAAAATGTGAGG+AGG	+	tig0027699:54956-54975	None:intergenic	30.0%
	AGTTTCCATGTTTCTTTGTT+TGG	+	tig0027699:55076-55095	None:intergenic	30.0%
	TTGTGATTAATGTCCTGTAT+AGG	-	tig0027699:55836-55855	Msa1435360:CDS	30.0%
	CTAGCCTATTTGTCTTTATT+TGG	+	tig0027699:55907-55926	None:intergenic	30.0%
	TGTGACAATCAAGAAAATCA+AGG	-	tig0027699:55956-55975	Msa1435360:CDS	30.0%
!	TTTGAGTTCATCAAGCAAAA+AGG	-	tig0027699:56007-56026	Msa1435360:intron	30.0%
!	TTTTTACCTCTGAGTAAAAC+TGG	+	tig0027699:56315-56334	None:intergenic	30.0%
	CCAATAATTAGTTCCATCAA+CGG	+	tig0027699:56556-56575	None:intergenic	30.0%
	GAACTAATTATTGGATTGTG+AGG	-	tig0027699:56562-56581	Msa1435360:CDS	30.0%
!!	TTTTGGAGGAGTTTTTAACT+GGG	+	tig0027699:56689-56708	None:intergenic	30.0%
!!	TTTTTGGAGGAGTTTTTAAC+TGG	+	tig0027699:56690-56709	None:intergenic	30.0%
!	TTTGATGACAACGTATGATT+TGG	-	tig0027699:56856-56875	Msa1435360:five_prime_UTR	30.0%
!	TTTAAGACATAATGGTTGCA+GGG	+	tig0027699:56965-56984	None:intergenic	30.0%
!!!	TTTTAAGACATAATGGTTGC+AGG	+	tig0027699:56966-56985	None:intergenic	30.0%
!	ATGTTTGTGTTGTTCAAGTA+TGG	+	tig0027699:54876-54895	None:intergenic	30.0%
	AAAAAATGTGAGGAGGTTAA+AGG	+	tig0027699:54949-54968	None:intergenic	30.0%
	CTCAATAAAAAAATGTGAGG+AGG	+	tig0027699:54956-54975	None:intergenic	30.0%
	AGTTTCCATGTTTCTTTGTT+TGG	+	tig0027699:55076-55095	None:intergenic	30.0%
	TTGTGATTAATGTCCTGTAT+AGG	-	tig0027699:55836-55855	Msa1435360:CDS	30.0%
	CTAGCCTATTTGTCTTTATT+TGG	+	tig0027699:55907-55926	None:intergenic	30.0%
	TGTGACAATCAAGAAAATCA+AGG	-	tig0027699:55956-55975	Msa1435360:CDS	30.0%
!	TTTGAGTTCATCAAGCAAAA+AGG	-	tig0027699:56007-56026	Msa1435360:intron	30.0%
!	TTTTTACCTCTGAGTAAAAC+TGG	+	tig0027699:56315-56334	None:intergenic	30.0%
	CCAATAATTAGTTCCATCAA+CGG	+	tig0027699:56556-56575	None:intergenic	30.0%
	GAACTAATTATTGGATTGTG+AGG	-	tig0027699:56562-56581	Msa1435360:CDS	30.0%
!!	TTTTGGAGGAGTTTTTAACT+GGG	+	tig0027699:56689-56708	None:intergenic	30.0%
!!	TTTTTGGAGGAGTTTTTAAC+TGG	+	tig0027699:56690-56709	None:intergenic	30.0%
!	TTTGATGACAACGTATGATT+TGG	-	tig0027699:56856-56875	Msa1435360:five_prime_UTR	30.0%
!	TTTAAGACATAATGGTTGCA+GGG	+	tig0027699:56965-56984	None:intergenic	30.0%
!!!	TTTTAAGACATAATGGTTGC+AGG	+	tig0027699:56966-56985	None:intergenic	30.0%
	TTGTTCAAGTATGGAAGCAA+AGG	+	tig0027699:54867-54886	None:intergenic	35.0%
	GAGGTTAAAGGTTTCTTAAG+GGG	+	tig0027699:54937-54956	None:intergenic	35.0%
	GGAGGTTAAAGGTTTCTTAA+GGG	+	tig0027699:54938-54957	None:intergenic	35.0%
	AGGAGGTTAAAGGTTTCTTA+AGG	+	tig0027699:54939-54958	None:intergenic	35.0%
	GAATGGAAGTTGGATGAAAA+GGG	+	tig0027699:55022-55041	None:intergenic	35.0%
	TGAATGGAAGTTGGATGAAA+AGG	+	tig0027699:55023-55042	None:intergenic	35.0%
	TTGGAAGCAATTCACTTGTT+TGG	+	tig0027699:55057-55076	None:intergenic	35.0%
	TGCTTCCAAACAAAGAAACA+TGG	-	tig0027699:55068-55087	Msa1435360:three_prime_UTR	35.0%
	TACTTCAAGAACCAAAGCAA+TGG	+	tig0027699:55124-55143	None:intergenic	35.0%
	TATCCATCTTGTTAGTGTTG+TGG	+	tig0027699:55299-55318	None:intergenic	35.0%
!	AAATTCATGGTTGGTCATGT+CGG	+	tig0027699:55352-55371	None:intergenic	35.0%
	ACATGACCAACCATGAATTT+AGG	-	tig0027699:55352-55371	Msa1435360:CDS	35.0%
	CATAGGTAGTCCTAAATTCA+TGG	+	tig0027699:55365-55384	None:intergenic	35.0%
	ATTAACCTTTGATCCAGCAT+AGG	+	tig0027699:55382-55401	None:intergenic	35.0%
!	TCTGTTGATTGGAGAAAGAA+AGG	-	tig0027699:55477-55496	Msa1435360:intron	35.0%
	GACACCATCTAATCAAAGAT+CGG	-	tig0027699:55600-55619	Msa1435360:CDS	35.0%
	ATAAGCTCTTGTTCAGACAA+AGG	+	tig0027699:55932-55951	None:intergenic	35.0%
	CAAGAAAATCAAGGATGCAA+TGG	-	tig0027699:55965-55984	Msa1435360:CDS	35.0%
	AAAATCAAGGATGCAATGGT+GGG	-	tig0027699:55969-55988	Msa1435360:CDS	35.0%
!!	ATCCATCATTTGCTGTGTAA+GGG	+	tig0027699:56057-56076	None:intergenic	35.0%
!	TGATTTCCAGTTTTACTCAG+AGG	-	tig0027699:56306-56325	Msa1435360:intron	35.0%
	ATTCTCTTGTGTCAATAGAG+AGG	+	tig0027699:56349-56368	None:intergenic	35.0%
	CACTAAATACTCCCTGTAAT+AGG	+	tig0027699:56479-56498	None:intergenic	35.0%
!!	CTATTACAGGGAGTATTTAG+TGG	-	tig0027699:56477-56496	Msa1435360:CDS	35.0%
	TGTGGTAAAGAGCTAAATCA+TGG	-	tig0027699:56504-56523	Msa1435360:CDS	35.0%
	AATCATGGTGTAGCAATAGT+AGG	-	tig0027699:56519-56538	Msa1435360:CDS	35.0%
!	CCGTTGATGGAACTAATTAT+TGG	-	tig0027699:56553-56572	Msa1435360:CDS	35.0%
	AAGGTTACATCAGAATGCAA+AGG	-	tig0027699:56613-56632	Msa1435360:CDS	35.0%
	GCAAAGGAATGTATCTAACA+AGG	-	tig0027699:56629-56648	Msa1435360:CDS	35.0%
	AGGAATGTATCTAACAAGGA+AGG	-	tig0027699:56633-56652	Msa1435360:CDS	35.0%
	TCATCCTTGGTAGATGATAA+AGG	+	tig0027699:56723-56742	None:intergenic	35.0%
	AAACATTCAAAGCTCATCCT+TGG	+	tig0027699:56736-56755	None:intergenic	35.0%
	TTGTTCAAGTATGGAAGCAA+AGG	+	tig0027699:54867-54886	None:intergenic	35.0%
	GAGGTTAAAGGTTTCTTAAG+GGG	+	tig0027699:54937-54956	None:intergenic	35.0%
	GGAGGTTAAAGGTTTCTTAA+GGG	+	tig0027699:54938-54957	None:intergenic	35.0%
	AGGAGGTTAAAGGTTTCTTA+AGG	+	tig0027699:54939-54958	None:intergenic	35.0%
	GAATGGAAGTTGGATGAAAA+GGG	+	tig0027699:55022-55041	None:intergenic	35.0%
	TGAATGGAAGTTGGATGAAA+AGG	+	tig0027699:55023-55042	None:intergenic	35.0%
	TTGGAAGCAATTCACTTGTT+TGG	+	tig0027699:55057-55076	None:intergenic	35.0%
	TGCTTCCAAACAAAGAAACA+TGG	-	tig0027699:55068-55087	Msa1435360:three_prime_UTR	35.0%
	TACTTCAAGAACCAAAGCAA+TGG	+	tig0027699:55124-55143	None:intergenic	35.0%
	TATCCATCTTGTTAGTGTTG+TGG	+	tig0027699:55299-55318	None:intergenic	35.0%
!	AAATTCATGGTTGGTCATGT+CGG	+	tig0027699:55352-55371	None:intergenic	35.0%
	ACATGACCAACCATGAATTT+AGG	-	tig0027699:55352-55371	Msa1435360:CDS	35.0%
	CATAGGTAGTCCTAAATTCA+TGG	+	tig0027699:55365-55384	None:intergenic	35.0%
	ATTAACCTTTGATCCAGCAT+AGG	+	tig0027699:55382-55401	None:intergenic	35.0%
!	TCTGTTGATTGGAGAAAGAA+AGG	-	tig0027699:55477-55496	Msa1435360:intron	35.0%
	GACACCATCTAATCAAAGAT+CGG	-	tig0027699:55600-55619	Msa1435360:CDS	35.0%
	ATAAGCTCTTGTTCAGACAA+AGG	+	tig0027699:55932-55951	None:intergenic	35.0%
	CAAGAAAATCAAGGATGCAA+TGG	-	tig0027699:55965-55984	Msa1435360:CDS	35.0%
	AAAATCAAGGATGCAATGGT+GGG	-	tig0027699:55969-55988	Msa1435360:CDS	35.0%
!!	ATCCATCATTTGCTGTGTAA+GGG	+	tig0027699:56057-56076	None:intergenic	35.0%
!	TGATTTCCAGTTTTACTCAG+AGG	-	tig0027699:56306-56325	Msa1435360:intron	35.0%
	ATTCTCTTGTGTCAATAGAG+AGG	+	tig0027699:56349-56368	None:intergenic	35.0%
	CACTAAATACTCCCTGTAAT+AGG	+	tig0027699:56479-56498	None:intergenic	35.0%
!!	CTATTACAGGGAGTATTTAG+TGG	-	tig0027699:56477-56496	Msa1435360:CDS	35.0%
	TGTGGTAAAGAGCTAAATCA+TGG	-	tig0027699:56504-56523	Msa1435360:CDS	35.0%
	AATCATGGTGTAGCAATAGT+AGG	-	tig0027699:56519-56538	Msa1435360:CDS	35.0%
!	CCGTTGATGGAACTAATTAT+TGG	-	tig0027699:56553-56572	Msa1435360:CDS	35.0%
	AAGGTTACATCAGAATGCAA+AGG	-	tig0027699:56613-56632	Msa1435360:CDS	35.0%
	GCAAAGGAATGTATCTAACA+AGG	-	tig0027699:56629-56648	Msa1435360:CDS	35.0%
	AGGAATGTATCTAACAAGGA+AGG	-	tig0027699:56633-56652	Msa1435360:CDS	35.0%
	TCATCCTTGGTAGATGATAA+AGG	+	tig0027699:56723-56742	None:intergenic	35.0%
	AAACATTCAAAGCTCATCCT+TGG	+	tig0027699:56736-56755	None:intergenic	35.0%
	GATTGTTCTGTCCATTGCTT+TGG	-	tig0027699:55110-55129	Msa1435360:exon	40.0%
	ATCTTGGAAATCGAAGCTCT+CGG	+	tig0027699:55148-55167	None:intergenic	40.0%
	CCAAGATAAAGATGTGTCCT+CGG	-	tig0027699:55161-55180	Msa1435360:CDS	40.0%
	TGTCCACAACACTAACAAGA+TGG	-	tig0027699:55293-55312	Msa1435360:CDS	40.0%
	GGTAGTCCTAAATTCATGGT+TGG	+	tig0027699:55361-55380	None:intergenic	40.0%
!	GAATTTAGGACTACCTATGC+TGG	-	tig0027699:55366-55385	Msa1435360:CDS	40.0%
	TTTCTCCAATCAACAGAAGC+AGG	+	tig0027699:55474-55493	None:intergenic	40.0%
	ACTGTGTGAATGCAAAGTGA+TGG	+	tig0027699:55571-55590	None:intergenic	40.0%
	TCGTCCGATCTTTGATTAGA+TGG	+	tig0027699:55607-55626	None:intergenic	40.0%
	TCAACTGTTGTAGCTGTTGA+AGG	-	tig0027699:55875-55894	Msa1435360:CDS	40.0%
	GAAAATCAAGGATGCAATGG+TGG	-	tig0027699:55968-55987	Msa1435360:CDS	40.0%
	GAGTTCATCAAGCAAAAAGG+TGG	-	tig0027699:56010-56029	Msa1435360:intron	40.0%
	TACCCTTACACAGCAAATGA+TGG	-	tig0027699:56052-56071	Msa1435360:intron	40.0%
!!	GATCCATCATTTGCTGTGTA+AGG	+	tig0027699:56058-56077	None:intergenic	40.0%
!	AAAGCATCTTCATCGTTAGC+CGG	+	tig0027699:56232-56251	None:intergenic	40.0%
	AAACTGGAAATCAGATCCTC+CGG	+	tig0027699:56299-56318	None:intergenic	40.0%
	GGTGTAGCAATAGTAGGATA+TGG	-	tig0027699:56525-56544	Msa1435360:CDS	40.0%
	ATTGGATTGTGAGGAACTCA+TGG	-	tig0027699:56571-56590	Msa1435360:CDS	40.0%
	TTGGATTGTGAGGAACTCAT+GGG	-	tig0027699:56572-56591	Msa1435360:CDS	40.0%
	TCTAACAAGGAAGGACTTTG+TGG	-	tig0027699:56642-56661	Msa1435360:CDS	40.0%
!	TAAAGGTCCTGCTGGATTTT+TGG	+	tig0027699:56706-56725	None:intergenic	40.0%
	AGGACCTTTATCATCTACCA+AGG	-	tig0027699:56716-56735	Msa1435360:CDS	40.0%
	ATAATGGTTGCAGGGAATGA+GGG	+	tig0027699:56957-56976	None:intergenic	40.0%
	GATTGTTCTGTCCATTGCTT+TGG	-	tig0027699:55110-55129	Msa1435360:exon	40.0%
	ATCTTGGAAATCGAAGCTCT+CGG	+	tig0027699:55148-55167	None:intergenic	40.0%
	CCAAGATAAAGATGTGTCCT+CGG	-	tig0027699:55161-55180	Msa1435360:CDS	40.0%
	TGTCCACAACACTAACAAGA+TGG	-	tig0027699:55293-55312	Msa1435360:CDS	40.0%
	GGTAGTCCTAAATTCATGGT+TGG	+	tig0027699:55361-55380	None:intergenic	40.0%
!	GAATTTAGGACTACCTATGC+TGG	-	tig0027699:55366-55385	Msa1435360:CDS	40.0%
	TTTCTCCAATCAACAGAAGC+AGG	+	tig0027699:55474-55493	None:intergenic	40.0%
	ACTGTGTGAATGCAAAGTGA+TGG	+	tig0027699:55571-55590	None:intergenic	40.0%
	TCGTCCGATCTTTGATTAGA+TGG	+	tig0027699:55607-55626	None:intergenic	40.0%
	TCAACTGTTGTAGCTGTTGA+AGG	-	tig0027699:55875-55894	Msa1435360:CDS	40.0%
	GAAAATCAAGGATGCAATGG+TGG	-	tig0027699:55968-55987	Msa1435360:CDS	40.0%
	GAGTTCATCAAGCAAAAAGG+TGG	-	tig0027699:56010-56029	Msa1435360:intron	40.0%
	TACCCTTACACAGCAAATGA+TGG	-	tig0027699:56052-56071	Msa1435360:intron	40.0%
!!	GATCCATCATTTGCTGTGTA+AGG	+	tig0027699:56058-56077	None:intergenic	40.0%
!	AAAGCATCTTCATCGTTAGC+CGG	+	tig0027699:56232-56251	None:intergenic	40.0%
	AAACTGGAAATCAGATCCTC+CGG	+	tig0027699:56299-56318	None:intergenic	40.0%
	GGTGTAGCAATAGTAGGATA+TGG	-	tig0027699:56525-56544	Msa1435360:CDS	40.0%
	ATTGGATTGTGAGGAACTCA+TGG	-	tig0027699:56571-56590	Msa1435360:CDS	40.0%
	TTGGATTGTGAGGAACTCAT+GGG	-	tig0027699:56572-56591	Msa1435360:CDS	40.0%
	TCTAACAAGGAAGGACTTTG+TGG	-	tig0027699:56642-56661	Msa1435360:CDS	40.0%
!	TAAAGGTCCTGCTGGATTTT+TGG	+	tig0027699:56706-56725	None:intergenic	40.0%
	AGGACCTTTATCATCTACCA+AGG	-	tig0027699:56716-56735	Msa1435360:CDS	40.0%
	ATAATGGTTGCAGGGAATGA+GGG	+	tig0027699:56957-56976	None:intergenic	40.0%
	GCAGGGGATGGTTTATATAG+AGG	+	tig0027699:54994-55013	None:intergenic	45.0%
	GATGAAAAGGGAAAGAGCAG+GGG	+	tig0027699:55010-55029	None:intergenic	45.0%
	GGATGAAAAGGGAAAGAGCA+GGG	+	tig0027699:55011-55030	None:intergenic	45.0%
	TGGATGAAAAGGGAAAGAGC+AGG	+	tig0027699:55012-55031	None:intergenic	45.0%
	CCGAGGACACATCTTTATCT+TGG	+	tig0027699:55164-55183	None:intergenic	45.0%
	TGTGGGACTTATACGAGAGA+TGG	-	tig0027699:55193-55212	Msa1435360:CDS	45.0%
!!	AGCGCTTTTGTTTCTCGTCA+AGG	+	tig0027699:55245-55264	None:intergenic	45.0%
	GACTACCTATGCTGGATCAA+AGG	-	tig0027699:55374-55393	Msa1435360:intron	45.0%
	AATCACCACAGAATGTTCCG+AGG	-	tig0027699:55399-55418	Msa1435360:intron	45.0%
	ATGAATGTTCCACTTCCACG+TGG	+	tig0027699:55429-55448	None:intergenic	45.0%
	ATCAACAGAAGCAGGAGCTT+TGG	+	tig0027699:55466-55485	None:intergenic	45.0%
	AAGCTCCTGCTTCTGTTGAT+TGG	-	tig0027699:55466-55485	Msa1435360:intron	45.0%
!!	AAAGAAAGGTGCTGTCACTG+AGG	-	tig0027699:55491-55510	Msa1435360:intron	45.0%
	TGTCCTGTATAGGTAGTTGC+TGG	-	tig0027699:55846-55865	Msa1435360:CDS	45.0%
	GTCCTGTATAGGTAGTTGCT+GGG	-	tig0027699:55847-55866	Msa1435360:CDS	45.0%
	GACCATCAATTGACACAGCT+GGG	+	tig0027699:56198-56217	None:intergenic	45.0%
	TGACCATCAATTGACACAGC+TGG	+	tig0027699:56199-56218	None:intergenic	45.0%
	TGATGGTCATGAGACTGTTC+CGG	-	tig0027699:56210-56229	Msa1435360:intron	45.0%
!	GGAGTATTTAGTGGCGATTG+TGG	-	tig0027699:56486-56505	Msa1435360:CDS	45.0%
	GGATATGGAACAACCGTTGA+TGG	-	tig0027699:56540-56559	Msa1435360:CDS	45.0%
	TGGATTGTGAGGAACTCATG+GGG	-	tig0027699:56573-56592	Msa1435360:CDS	45.0%
	AGGACTTTGTGGCATAGCTA+TGG	-	tig0027699:56653-56672	Msa1435360:CDS	45.0%
!!	GGAGGAGTTTTTAACTGGGT+AGG	+	tig0027699:56685-56704	None:intergenic	45.0%
	AACTCCTCCAAAAATCCAGC+AGG	-	tig0027699:56696-56715	Msa1435360:CDS	45.0%
	GTAGATGATAAAGGTCCTGC+TGG	+	tig0027699:56714-56733	None:intergenic	45.0%
	CATAATGGTTGCAGGGAATG+AGG	+	tig0027699:56958-56977	None:intergenic	45.0%
	GCAGGGGATGGTTTATATAG+AGG	+	tig0027699:54994-55013	None:intergenic	45.0%
	GATGAAAAGGGAAAGAGCAG+GGG	+	tig0027699:55010-55029	None:intergenic	45.0%
	GGATGAAAAGGGAAAGAGCA+GGG	+	tig0027699:55011-55030	None:intergenic	45.0%
	TGGATGAAAAGGGAAAGAGC+AGG	+	tig0027699:55012-55031	None:intergenic	45.0%
	CCGAGGACACATCTTTATCT+TGG	+	tig0027699:55164-55183	None:intergenic	45.0%
	TGTGGGACTTATACGAGAGA+TGG	-	tig0027699:55193-55212	Msa1435360:CDS	45.0%
!!	AGCGCTTTTGTTTCTCGTCA+AGG	+	tig0027699:55245-55264	None:intergenic	45.0%
	GACTACCTATGCTGGATCAA+AGG	-	tig0027699:55374-55393	Msa1435360:intron	45.0%
	AATCACCACAGAATGTTCCG+AGG	-	tig0027699:55399-55418	Msa1435360:intron	45.0%
	ATGAATGTTCCACTTCCACG+TGG	+	tig0027699:55429-55448	None:intergenic	45.0%
	ATCAACAGAAGCAGGAGCTT+TGG	+	tig0027699:55466-55485	None:intergenic	45.0%
	AAGCTCCTGCTTCTGTTGAT+TGG	-	tig0027699:55466-55485	Msa1435360:intron	45.0%
!!	AAAGAAAGGTGCTGTCACTG+AGG	-	tig0027699:55491-55510	Msa1435360:intron	45.0%
	TGTCCTGTATAGGTAGTTGC+TGG	-	tig0027699:55846-55865	Msa1435360:CDS	45.0%
	GTCCTGTATAGGTAGTTGCT+GGG	-	tig0027699:55847-55866	Msa1435360:CDS	45.0%
	GACCATCAATTGACACAGCT+GGG	+	tig0027699:56198-56217	None:intergenic	45.0%
	TGACCATCAATTGACACAGC+TGG	+	tig0027699:56199-56218	None:intergenic	45.0%
	TGATGGTCATGAGACTGTTC+CGG	-	tig0027699:56210-56229	Msa1435360:intron	45.0%
!	GGAGTATTTAGTGGCGATTG+TGG	-	tig0027699:56486-56505	Msa1435360:CDS	45.0%
	GGATATGGAACAACCGTTGA+TGG	-	tig0027699:56540-56559	Msa1435360:CDS	45.0%
	TGGATTGTGAGGAACTCATG+GGG	-	tig0027699:56573-56592	Msa1435360:CDS	45.0%
	AGGACTTTGTGGCATAGCTA+TGG	-	tig0027699:56653-56672	Msa1435360:CDS	45.0%
!!	GGAGGAGTTTTTAACTGGGT+AGG	+	tig0027699:56685-56704	None:intergenic	45.0%
	AACTCCTCCAAAAATCCAGC+AGG	-	tig0027699:56696-56715	Msa1435360:CDS	45.0%
	GTAGATGATAAAGGTCCTGC+TGG	+	tig0027699:56714-56733	None:intergenic	45.0%
	CATAATGGTTGCAGGGAATG+AGG	+	tig0027699:56958-56977	None:intergenic	45.0%
	AAAAGGGAAAGAGCAGGGGA+TGG	+	tig0027699:55006-55025	None:intergenic	50.0%
	TGTCCTCGGACAAAAGCTTG+TGG	-	tig0027699:55175-55194	Msa1435360:CDS	50.0%
	GTCCTCGGACAAAAGCTTGT+GGG	-	tig0027699:55176-55195	Msa1435360:CDS	50.0%
	GGGACTTATACGAGAGATGG+AGG	-	tig0027699:55196-55215	Msa1435360:CDS	50.0%
	GTCACTGAGGTCAAGGATCA+AGG	-	tig0027699:55504-55523	Msa1435360:intron	50.0%
	GTCAAGGATCAAGGCCAATG+TGG	-	tig0027699:55513-55532	Msa1435360:intron	50.0%
	AGCCCAGCAACTACCTATAC+AGG	+	tig0027699:55852-55871	None:intergenic	50.0%
!!	AGGATGCAATGGTGGGCTAA+TGG	-	tig0027699:55976-55995	Msa1435360:CDS	50.0%
	GTCCCAGCTGTGTCAATTGA+TGG	-	tig0027699:56193-56212	Msa1435360:intron	50.0%
	CTATGGCTACGGATACAGGT+TGG	+	tig0027699:56273-56292	None:intergenic	50.0%
	GCATCTATGGCTACGGATAC+AGG	+	tig0027699:56277-56296	None:intergenic	50.0%
	GTATCCGTAGCCATAGATGC+CGG	-	tig0027699:56277-56296	Msa1435360:intron	50.0%
	ATCAGATCCTCCGGCATCTA+TGG	+	tig0027699:56290-56309	None:intergenic	50.0%
!!	AGGTCCTGCTGGATTTTTGG+AGG	+	tig0027699:56703-56722	None:intergenic	50.0%
	AAAAGGGAAAGAGCAGGGGA+TGG	+	tig0027699:55006-55025	None:intergenic	50.0%
	TGTCCTCGGACAAAAGCTTG+TGG	-	tig0027699:55175-55194	Msa1435360:CDS	50.0%
	GTCCTCGGACAAAAGCTTGT+GGG	-	tig0027699:55176-55195	Msa1435360:CDS	50.0%
	GGGACTTATACGAGAGATGG+AGG	-	tig0027699:55196-55215	Msa1435360:CDS	50.0%
	GTCACTGAGGTCAAGGATCA+AGG	-	tig0027699:55504-55523	Msa1435360:intron	50.0%
	GTCAAGGATCAAGGCCAATG+TGG	-	tig0027699:55513-55532	Msa1435360:intron	50.0%
	AGCCCAGCAACTACCTATAC+AGG	+	tig0027699:55852-55871	None:intergenic	50.0%
!!	AGGATGCAATGGTGGGCTAA+TGG	-	tig0027699:55976-55995	Msa1435360:CDS	50.0%
	GTCCCAGCTGTGTCAATTGA+TGG	-	tig0027699:56193-56212	Msa1435360:intron	50.0%
	CTATGGCTACGGATACAGGT+TGG	+	tig0027699:56273-56292	None:intergenic	50.0%
	GCATCTATGGCTACGGATAC+AGG	+	tig0027699:56277-56296	None:intergenic	50.0%
	GTATCCGTAGCCATAGATGC+CGG	-	tig0027699:56277-56296	Msa1435360:intron	50.0%
	ATCAGATCCTCCGGCATCTA+TGG	+	tig0027699:56290-56309	None:intergenic	50.0%
!!	AGGTCCTGCTGGATTTTTGG+AGG	+	tig0027699:56703-56722	None:intergenic	50.0%
!	GTCCCACAAGCTTTTGTCCG+AGG	+	tig0027699:55181-55200	None:intergenic	55.0%
	GAGATGGAGGAGTCACCACA+CGG	-	tig0027699:55209-55228	Msa1435360:CDS	55.0%
	GGAGTCACCACACGGTTTCT+CGG	-	tig0027699:55217-55236	Msa1435360:CDS	55.0%
	CAAGGTTCCGAGAAACCGTG+TGG	+	tig0027699:55227-55246	None:intergenic	55.0%
!	AGGTGCTGTCACTGAGGTCA+AGG	-	tig0027699:55497-55516	Msa1435360:intron	55.0%
!!	GGCTACGGATACAGGTTGGT+TGG	+	tig0027699:56269-56288	None:intergenic	55.0%
	TCCTCCGGCATCTATGGCTA+CGG	+	tig0027699:56284-56303	None:intergenic	55.0%
	TGTGAGGAACTCATGGGGAG+CGG	-	tig0027699:56578-56597	Msa1435360:CDS	55.0%
	GAACTCATGGGGAGCGGAAT+GGG	-	tig0027699:56584-56603	Msa1435360:CDS	55.0%
	AACTCATGGGGAGCGGAATG+GGG	-	tig0027699:56585-56604	Msa1435360:CDS	55.0%
!	GTCCCACAAGCTTTTGTCCG+AGG	+	tig0027699:55181-55200	None:intergenic	55.0%
	GAGATGGAGGAGTCACCACA+CGG	-	tig0027699:55209-55228	Msa1435360:CDS	55.0%
	GGAGTCACCACACGGTTTCT+CGG	-	tig0027699:55217-55236	Msa1435360:CDS	55.0%
	CAAGGTTCCGAGAAACCGTG+TGG	+	tig0027699:55227-55246	None:intergenic	55.0%
!	AGGTGCTGTCACTGAGGTCA+AGG	-	tig0027699:55497-55516	Msa1435360:intron	55.0%
!!	GGCTACGGATACAGGTTGGT+TGG	+	tig0027699:56269-56288	None:intergenic	55.0%
	TCCTCCGGCATCTATGGCTA+CGG	+	tig0027699:56284-56303	None:intergenic	55.0%
	TGTGAGGAACTCATGGGGAG+CGG	-	tig0027699:56578-56597	Msa1435360:CDS	55.0%
	GAACTCATGGGGAGCGGAAT+GGG	-	tig0027699:56584-56603	Msa1435360:CDS	55.0%
	AACTCATGGGGAGCGGAATG+GGG	-	tig0027699:56585-56604	Msa1435360:CDS	55.0%
	GCGTGCCTCGGAACATTCTG+TGG	+	tig0027699:55407-55426	None:intergenic	60.0%
	TCCGTAGCCATAGATGCCGG+AGG	-	tig0027699:56280-56299	Msa1435360:intron	60.0%
	GGAACTCATGGGGAGCGGAA+TGG	-	tig0027699:56583-56602	Msa1435360:CDS	60.0%
	GGAGCGGAATGGGGTGAACA+AGG	-	tig0027699:56594-56613	Msa1435360:CDS	60.0%
	GCGTGCCTCGGAACATTCTG+TGG	+	tig0027699:55407-55426	None:intergenic	60.0%
	TCCGTAGCCATAGATGCCGG+AGG	-	tig0027699:56280-56299	Msa1435360:intron	60.0%
	GGAACTCATGGGGAGCGGAA+TGG	-	tig0027699:56583-56602	Msa1435360:CDS	60.0%
	GGAGCGGAATGGGGTGAACA+AGG	-	tig0027699:56594-56613	Msa1435360:CDS	60.0%
	ATGTTCCGAGGCACGCCACG+TGG	-	tig0027699:55411-55430	Msa1435360:intron	65.0%
	CACTTCCACGTGGCGTGCCT+CGG	+	tig0027699:55419-55438	None:intergenic	65.0%
	ATGTTCCGAGGCACGCCACG+TGG	-	tig0027699:55411-55430	Msa1435360:intron	65.0%
	CACTTCCACGTGGCGTGCCT+CGG	+	tig0027699:55419-55438	None:intergenic	65.0%
	CGAGGCACGCCACGTGGAAG+TGG	-	tig0027699:55417-55436	Msa1435360:intron	70.0%
	CGAGGCACGCCACGTGGAAG+TGG	-	tig0027699:55417-55436	Msa1435360:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
tig0027699	gene	54860	57015	54860	ID=Msa1435360;Name=Msa1435360
tig0027699	mRNA	54860	57015	54860	ID=Msa1435360-mRNA-1;Parent=Msa1435360;Name=Msa1435360-mRNA-1;_AED=0.03;_eAED=0.03;_QI=227\|1\|1\|1\|1\|1\|4\|266\|362
tig0027699	exon	54860	55389	54860	ID=Msa1435360-mRNA-1:exon:9622;Parent=Msa1435360-mRNA-1
tig0027699	exon	55548	55688	55548	ID=Msa1435360-mRNA-1:exon:9621;Parent=Msa1435360-mRNA-1
tig0027699	exon	55782	56017	55782	ID=Msa1435360-mRNA-1:exon:9620;Parent=Msa1435360-mRNA-1
tig0027699	exon	56341	57015	56341	ID=Msa1435360-mRNA-1:exon:9619;Parent=Msa1435360-mRNA-1
tig0027699	five_prime_UTR	56789	57015	56789	ID=Msa1435360-mRNA-1:five_prime_utr;Parent=Msa1435360-mRNA-1
tig0027699	CDS	56341	56788	56341	ID=Msa1435360-mRNA-1:cds;Parent=Msa1435360-mRNA-1
tig0027699	CDS	55782	56017	55782	ID=Msa1435360-mRNA-1:cds;Parent=Msa1435360-mRNA-1
tig0027699	CDS	55548	55688	55548	ID=Msa1435360-mRNA-1:cds;Parent=Msa1435360-mRNA-1
tig0027699	CDS	55126	55389	55126	ID=Msa1435360-mRNA-1:cds;Parent=Msa1435360-mRNA-1
tig0027699	three_prime_UTR	54860	55125	54860	ID=Msa1435360-mRNA-1:three_prime_utr;Parent=Msa1435360-mRNA-1

Gene Sequence

>Msa1435360

ATGGAAACTAAGAAACTATTGTTGATTGTTCTGTCCATTGCTTTGGTTCTTGAAGTATCCGAGAGCTTCGATTTCCAAGATAAAGATGTGTCCTCGGACAAAAGCTTGTGGGACTTATACGAGAGATGGAGGAGTCACCACACGGTTTCTCGGAACCTTGACGAGAAACAAAAGCGCTTTAACGTGTTCAAATCAAATGTGATACATGTCCACAACACTAACAAGATGGATAAGCCTTACAAGTTGAAACTTAACAAGTTTGCCGACATGACCAACCATGAATTTAGGACTACCTATGCTGGATCAAAGGTTAATCACCACAGAATGTTCCGAGGCACGCCACGTGGAAGTGGAACATTCATGTATGAAAATTTTACCAAAGCTCCTGCTTCTGTTGATTGGAGAAAGAAAGGTGCTGTCACTGAGGTCAAGGATCAAGGCCAATGTGGTAGTTGCTGGGCTTTTTCAACTGTTGTAGCTGTTGAAGGCATAAACCAAATAAAGACAAATAGGCTAGTGCCTTTGTCTGAACAAGAGCTTATAGATTGTGACAATCAAGAAAATCAAGGATGCAATGGTGGGCTAATGGAATATGCTTTTGAGTTCATCAAGCAAAAAGGTGGCATAACAACAGAAAGCTATTACCCTTACACAGCAAATGATGGATCATGTGATGCAACAAAAGAAAATGTCCCAGCTGTGTCAATTGATGGTCATGAGACTGTTCCGGCTAACGATGAAGATGCTTTGCTCAAAGCTGTTGCCAACCAACCTGTATCCGTAGCCATAGATGCCGGAGGATCTGATTTCCAGTTTTACTCAGAGGGAGTATTTAGTGGCGATTGTGGTAAAGAGCTAAATCATGGTGTAGCAATAGTAGGATATGGAACAACCGTTGATGGAACTAATTATTGGATTGTGAGGAACTCATGGGGAGCGGAATGGGGTGAACAAGGTTACATCAGAATGCAAAGGAATGTATCTAACAAGGAAGGACTTTGTGGCATAGCTATGGAAGCTTCCTACCCAGTTAAAAACTCCTCCAAAAATCCAGCAGGACCTTTATCATCTACCAAGGATGAGCTTTGA

Protein sequence

>Msa1435360

METKKLLLIVLSIALVLEVSESFDFQDKDVSSDKSLWDLYERWRSHHTVSRNLDEKQKRFNVFKSNVIHVHNTNKMDKPYKLKLNKFADMTNHEFRTTYAGSKVNHHRMFRGTPRGSGTFMYENFTKAPASVDWRKKGAVTEVKDQGQCGSCWAFSTVVAVEGINQIKTNRLVPLSEQELIDCDNQENQGCNGGLMEYAFEFIKQKGGITTESYYPYTANDGSCDATKENVPAVSIDGHETVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFSGDCGKELNHGVAIVGYGTTVDGTNYWIVRNSWGAEWGEQGYIRMQRNVSNKEGLCGIAMEASYPVKNSSKNPAGPLSSTKDEL*