AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1461080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461080 MtrunA17_Chr1g0184451 100.000 81 0 0 1 81 1 81 5.82e-53 166
Msa1461080 MtrunA17_Chr6g0485031 50.000 56 27 1 21 76 176 230 9.30e-12 58.9
Msa1461080 MtrunA17_Chr1g0153571 51.724 58 27 1 17 74 54 110 1.27e-11 58.2
Msa1461080 MtrunA17_Chr1g0151421 51.724 58 27 1 17 74 55 111 1.27e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461080 AT4G19540.1 76.119 67 16 0 15 81 36 102 2.49e-30 108
Msa1461080 AT3G24430.1 53.571 56 25 1 21 76 175 229 1.05e-12 62.0

Find 30 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGATTCAACACATAGAT+TGG 0.347912 tig0041264:+1955 None:intergenic
GATACCATAGCTATTGCTTC+TGG 0.358686 tig0041264:-1654 Msa1461080:CDS
GCGAAGCATCTTCGCATTGA+TGG 0.362197 tig0041264:-1684 Msa1461080:CDS
GTGTCGATGTTCATCATGAT+AGG 0.396838 tig0041264:+538 None:intergenic
GATATACCTTGAGTGACTTC+AGG 0.399695 tig0041264:+511 None:intergenic
GTAAGTTCCAATTGAAGGTT+GGG 0.411148 tig0041264:-594 Msa1461080:CDS
TGCAAGTAAGTTCCAATTGA+AGG 0.420623 tig0041264:-599 Msa1461080:CDS
CAAGTAACCCAACCTTCAAT+TGG 0.422756 tig0041264:+587 None:intergenic
GTTCATCATGATAGGAATGT+TGG 0.432777 tig0041264:+546 None:intergenic
GATCTTCTGTATGTGATGTC+GGG 0.446456 tig0041264:+1870 None:intergenic
ATTGATTCAACACATAGATT+GGG 0.450290 tig0041264:+1956 None:intergenic
TTACTTGATGCTGATGTGTA+TGG 0.464843 tig0041264:-571 Msa1461080:CDS
ATAGCTATTGCTTCTGGTAA+AGG 0.466248 tig0041264:-1648 Msa1461080:CDS
TTCATCATGATAGGAATGTT+GGG 0.479590 tig0041264:+547 None:intergenic
GCACTTTGTTTAGTTTGAGT+TGG 0.486889 tig0041264:+2026 None:intergenic
GGATCTTCTGTATGTGATGT+CGG 0.492796 tig0041264:+1869 None:intergenic
AGTAAGTTCCAATTGAAGGT+TGG 0.499750 tig0041264:-595 Msa1461080:CDS
CATCAAAGTTGATGAGGCCG+AGG 0.513994 tig0041264:-1830 Msa1461080:exon
ATCTTCTGTATGTGATGTCG+GGG 0.543008 tig0041264:+1871 None:intergenic
GTCGGCAAATCAACAACTGC+TGG 0.546887 tig0041264:-1621 Msa1461080:intron
TACAATTACATTCAACAGCA+AGG 0.547823 tig0041264:+1980 None:intergenic
TTTCATTCTGATCAGTGGCT+AGG 0.565317 tig0041264:-1723 Msa1461080:intron
GCTTCTGGTAAAGGCGGTGT+CGG 0.565622 tig0041264:-1639 Msa1461080:CDS
TTACATTCAACAGCAAGGAA+TGG 0.615847 tig0041264:+1985 None:intergenic
TTTACCAGAAGCAATAGCTA+TGG 0.617990 tig0041264:+1650 None:intergenic
CATTCACATCAAAGTTGATG+AGG 0.635095 tig0041264:-1836 Msa1461080:exon
ATCAAAGTTGATGAGGCCGA+GGG 0.661654 tig0041264:-1829 Msa1461080:intron
GCTATTGCTTCTGGTAAAGG+CGG 0.674617 tig0041264:-1645 Msa1461080:CDS
ACAAAGCCTGAAGTCACTCA+AGG 0.675264 tig0041264:-517 None:intergenic
TCTTCTGTATGTGATGTCGG+GGG 0.721310 tig0041264:+1872 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAAATTAAGATTGC+AGG - tig0041264:1388-1407 Msa1461080:intron 15.0%
!!! TTTTTTAAATTAAGATTGCA+GGG - tig0041264:1389-1408 Msa1461080:intron 15.0%
!!! TTTTTTTAAATTAAGATTGC+AGG - tig0041264:1388-1407 Msa1461080:intron 15.0%
!!! TTTTTTAAATTAAGATTGCA+GGG - tig0041264:1389-1408 Msa1461080:intron 15.0%
!!! CAAATTAGAAACAATTTTAG+AGG + tig0041264:1122-1141 None:intergenic 20.0%
!!! ATTGTTTCTAATTTGAAGTT+AGG - tig0041264:1127-1146 Msa1461080:intron 20.0%
!!! TTTTTAAATTAAGATTGCAG+GGG - tig0041264:1390-1409 Msa1461080:intron 20.0%
!!! CAAATTAGAAACAATTTTAG+AGG + tig0041264:1122-1141 None:intergenic 20.0%
!!! ATTGTTTCTAATTTGAAGTT+AGG - tig0041264:1127-1146 Msa1461080:intron 20.0%
!!! TTTTTAAATTAAGATTGCAG+GGG - tig0041264:1390-1409 Msa1461080:intron 20.0%
!! ATTGATTCAACACATAGATT+GGG + tig0041264:601-620 None:intergenic 25.0%
!! AATTGATTCAACACATAGAT+TGG + tig0041264:602-621 None:intergenic 25.0%
!!! TTTTGATTAGGTTGAATTTG+AGG + tig0041264:644-663 None:intergenic 25.0%
! GATATAAGCATATGTTTGAT+AGG - tig0041264:1066-1085 Msa1461080:intron 25.0%
! GTAGTGTTTGATAAAATTAG+CGG - tig0041264:1309-1328 Msa1461080:intron 25.0%
! GGGTTGAACAATAAATTTAA+GGG + tig0041264:1589-1608 None:intergenic 25.0%
!! TGGGTTGAACAATAAATTTA+AGG + tig0041264:1590-1609 None:intergenic 25.0%
! TTATTTCCATAAGTTCTTCA+GGG + tig0041264:1754-1773 None:intergenic 25.0%
!! ATTGTTTTCATAAGTTCTCT+CGG - tig0041264:1791-1810 Msa1461080:intron 25.0%
!! ATTGATTCAACACATAGATT+GGG + tig0041264:601-620 None:intergenic 25.0%
!! AATTGATTCAACACATAGAT+TGG + tig0041264:602-621 None:intergenic 25.0%
!!! TTTTGATTAGGTTGAATTTG+AGG + tig0041264:644-663 None:intergenic 25.0%
! GATATAAGCATATGTTTGAT+AGG - tig0041264:1066-1085 Msa1461080:intron 25.0%
! GTAGTGTTTGATAAAATTAG+CGG - tig0041264:1309-1328 Msa1461080:intron 25.0%
! GGGTTGAACAATAAATTTAA+GGG + tig0041264:1589-1608 None:intergenic 25.0%
!! TGGGTTGAACAATAAATTTA+AGG + tig0041264:1590-1609 None:intergenic 25.0%
! TTATTTCCATAAGTTCTTCA+GGG + tig0041264:1754-1773 None:intergenic 25.0%
!! ATTGTTTTCATAAGTTCTCT+CGG - tig0041264:1791-1810 Msa1461080:intron 25.0%
TACAATTACATTCAACAGCA+AGG + tig0041264:577-596 None:intergenic 30.0%
!!! GTGGATTTGAGATTTTGATT+AGG + tig0041264:656-675 None:intergenic 30.0%
! TTGATGTGAATGTTTTCTCA+GGG + tig0041264:710-729 None:intergenic 30.0%
! TTTGATGTGAATGTTTTCTC+AGG + tig0041264:711-730 None:intergenic 30.0%
AAGCATATGTTTGATAGGTT+TGG - tig0041264:1071-1090 Msa1461080:intron 30.0%
! ATATGTTTGATAGGTTTGGT+TGG - tig0041264:1075-1094 Msa1461080:intron 30.0%
! CTTTGTTTGCTAAAGAATAG+CGG - tig0041264:1191-1210 Msa1461080:intron 30.0%
GAAAATGACAATTACCAAAC+AGG - tig0041264:1496-1515 Msa1461080:intron 30.0%
AAGACACATATTTGAGCTTA+TGG + tig0041264:1556-1575 None:intergenic 30.0%
AAATATGTGTCTTACCAAAC+AGG - tig0041264:1563-1582 Msa1461080:intron 30.0%
AATAAATTTAAGGGCCTGTT+TGG + tig0041264:1580-1599 None:intergenic 30.0%
GGATACATTTACACAAAAGT+GGG + tig0041264:1609-1628 None:intergenic 30.0%
AATGTAAAGTGGTTTCTGTT+CGG + tig0041264:1630-1649 None:intergenic 30.0%
AAAGTGAGAAGAATGTAAAG+TGG + tig0041264:1641-1660 None:intergenic 30.0%
CTTATTTCCATAAGTTCTTC+AGG + tig0041264:1755-1774 None:intergenic 30.0%
! TCAACTAATAAGAGCTTGTT+TGG + tig0041264:1872-1891 None:intergenic 30.0%
TTCATCATGATAGGAATGTT+GGG + tig0041264:2010-2029 None:intergenic 30.0%
TACAATTACATTCAACAGCA+AGG + tig0041264:577-596 None:intergenic 30.0%
!!! GTGGATTTGAGATTTTGATT+AGG + tig0041264:656-675 None:intergenic 30.0%
! TTGATGTGAATGTTTTCTCA+GGG + tig0041264:710-729 None:intergenic 30.0%
! TTTGATGTGAATGTTTTCTC+AGG + tig0041264:711-730 None:intergenic 30.0%
AAGCATATGTTTGATAGGTT+TGG - tig0041264:1071-1090 Msa1461080:intron 30.0%
! ATATGTTTGATAGGTTTGGT+TGG - tig0041264:1075-1094 Msa1461080:intron 30.0%
! CTTTGTTTGCTAAAGAATAG+CGG - tig0041264:1191-1210 Msa1461080:intron 30.0%
GAAAATGACAATTACCAAAC+AGG - tig0041264:1496-1515 Msa1461080:intron 30.0%
AAGACACATATTTGAGCTTA+TGG + tig0041264:1556-1575 None:intergenic 30.0%
AAATATGTGTCTTACCAAAC+AGG - tig0041264:1563-1582 Msa1461080:intron 30.0%
AATAAATTTAAGGGCCTGTT+TGG + tig0041264:1580-1599 None:intergenic 30.0%
GGATACATTTACACAAAAGT+GGG + tig0041264:1609-1628 None:intergenic 30.0%
AATGTAAAGTGGTTTCTGTT+CGG + tig0041264:1630-1649 None:intergenic 30.0%
AAAGTGAGAAGAATGTAAAG+TGG + tig0041264:1641-1660 None:intergenic 30.0%
CTTATTTCCATAAGTTCTTC+AGG + tig0041264:1755-1774 None:intergenic 30.0%
! TCAACTAATAAGAGCTTGTT+TGG + tig0041264:1872-1891 None:intergenic 30.0%
TTCATCATGATAGGAATGTT+GGG + tig0041264:2010-2029 None:intergenic 30.0%
!! GCACTTTGTTTAGTTTGAGT+TGG + tig0041264:531-550 None:intergenic 35.0%
TTACATTCAACAGCAAGGAA+TGG + tig0041264:572-591 None:intergenic 35.0%
! TGATGTGAATGTTTTCTCAG+GGG + tig0041264:709-728 None:intergenic 35.0%
!! CATTCACATCAAAGTTGATG+AGG - tig0041264:718-737 Msa1461080:intron 35.0%
TATCGTTTCATTCTGATCAG+TGG - tig0041264:826-845 Msa1461080:intron 35.0%
TTTACCAGAAGCAATAGCTA+TGG + tig0041264:907-926 None:intergenic 35.0%
ATAGCTATTGCTTCTGGTAA+AGG - tig0041264:906-925 Msa1461080:intron 35.0%
GCTATAAGCTGCAAATTGAA+GGG + tig0041264:967-986 None:intergenic 35.0%
TGCTATAAGCTGCAAATTGA+AGG + tig0041264:968-987 None:intergenic 35.0%
!!! TGGTTGGTTTTGAGTTGAAT+TGG - tig0041264:1091-1110 Msa1461080:intron 35.0%
ATGACTGAAAAAGACCTGTT+TGG + tig0041264:1513-1532 None:intergenic 35.0%
CGGATACATTTACACAAAAG+TGG + tig0041264:1610-1629 None:intergenic 35.0%
TGCAAGTAAGTTCCAATTGA+AGG - tig0041264:1955-1974 Msa1461080:five_prime_UTR 35.0%
AGTAAGTTCCAATTGAAGGT+TGG - tig0041264:1959-1978 Msa1461080:five_prime_UTR 35.0%
GTAAGTTCCAATTGAAGGTT+GGG - tig0041264:1960-1979 Msa1461080:five_prime_UTR 35.0%
TTACTTGATGCTGATGTGTA+TGG - tig0041264:1983-2002 Msa1461080:five_prime_UTR 35.0%
GTTCATCATGATAGGAATGT+TGG + tig0041264:2011-2030 None:intergenic 35.0%
!! GCACTTTGTTTAGTTTGAGT+TGG + tig0041264:531-550 None:intergenic 35.0%
TTACATTCAACAGCAAGGAA+TGG + tig0041264:572-591 None:intergenic 35.0%
! TGATGTGAATGTTTTCTCAG+GGG + tig0041264:709-728 None:intergenic 35.0%
!! CATTCACATCAAAGTTGATG+AGG - tig0041264:718-737 Msa1461080:intron 35.0%
TATCGTTTCATTCTGATCAG+TGG - tig0041264:826-845 Msa1461080:intron 35.0%
TTTACCAGAAGCAATAGCTA+TGG + tig0041264:907-926 None:intergenic 35.0%
ATAGCTATTGCTTCTGGTAA+AGG - tig0041264:906-925 Msa1461080:intron 35.0%
GCTATAAGCTGCAAATTGAA+GGG + tig0041264:967-986 None:intergenic 35.0%
TGCTATAAGCTGCAAATTGA+AGG + tig0041264:968-987 None:intergenic 35.0%
!!! TGGTTGGTTTTGAGTTGAAT+TGG - tig0041264:1091-1110 Msa1461080:intron 35.0%
ATGACTGAAAAAGACCTGTT+TGG + tig0041264:1513-1532 None:intergenic 35.0%
CGGATACATTTACACAAAAG+TGG + tig0041264:1610-1629 None:intergenic 35.0%
TGCAAGTAAGTTCCAATTGA+AGG - tig0041264:1955-1974 Msa1461080:five_prime_UTR 35.0%
AGTAAGTTCCAATTGAAGGT+TGG - tig0041264:1959-1978 Msa1461080:five_prime_UTR 35.0%
GTAAGTTCCAATTGAAGGTT+GGG - tig0041264:1960-1979 Msa1461080:five_prime_UTR 35.0%
TTACTTGATGCTGATGTGTA+TGG - tig0041264:1983-2002 Msa1461080:five_prime_UTR 35.0%
GTTCATCATGATAGGAATGT+TGG + tig0041264:2011-2030 None:intergenic 35.0%
ATCTTCTGTATGTGATGTCG+GGG + tig0041264:686-705 None:intergenic 40.0%
GATCTTCTGTATGTGATGTC+GGG + tig0041264:687-706 None:intergenic 40.0%
GGATCTTCTGTATGTGATGT+CGG + tig0041264:688-707 None:intergenic 40.0%
TTTCATTCTGATCAGTGGCT+AGG - tig0041264:831-850 Msa1461080:intron 40.0%
GATACCATAGCTATTGCTTC+TGG - tig0041264:900-919 Msa1461080:intron 40.0%
! ACTGATAAGCTAGCTTGTAG+CGG - tig0041264:1217-1236 Msa1461080:intron 40.0%
AAGCTACCCTGAAGAACTTA+TGG - tig0041264:1745-1764 Msa1461080:intron 40.0%
CAAGTAACCCAACCTTCAAT+TGG + tig0041264:1970-1989 None:intergenic 40.0%
! GTGTCGATGTTCATCATGAT+AGG + tig0041264:2019-2038 None:intergenic 40.0%
ATCTTCTGTATGTGATGTCG+GGG + tig0041264:686-705 None:intergenic 40.0%
GATCTTCTGTATGTGATGTC+GGG + tig0041264:687-706 None:intergenic 40.0%
GGATCTTCTGTATGTGATGT+CGG + tig0041264:688-707 None:intergenic 40.0%
TTTCATTCTGATCAGTGGCT+AGG - tig0041264:831-850 Msa1461080:intron 40.0%
GATACCATAGCTATTGCTTC+TGG - tig0041264:900-919 Msa1461080:intron 40.0%
! ACTGATAAGCTAGCTTGTAG+CGG - tig0041264:1217-1236 Msa1461080:intron 40.0%
AAGCTACCCTGAAGAACTTA+TGG - tig0041264:1745-1764 Msa1461080:intron 40.0%
CAAGTAACCCAACCTTCAAT+TGG + tig0041264:1970-1989 None:intergenic 40.0%
! GTGTCGATGTTCATCATGAT+AGG + tig0041264:2019-2038 None:intergenic 40.0%
TCTTCTGTATGTGATGTCGG+GGG + tig0041264:685-704 None:intergenic 45.0%
!! ATCAAAGTTGATGAGGCCGA+GGG - tig0041264:725-744 Msa1461080:intron 45.0%
AAATGTCTCAGTCTCACCCT+CGG + tig0041264:744-763 None:intergenic 45.0%
GCTATTGCTTCTGGTAAAGG+CGG - tig0041264:909-928 Msa1461080:intron 45.0%
TCTTCTGTATGTGATGTCGG+GGG + tig0041264:685-704 None:intergenic 45.0%
!! ATCAAAGTTGATGAGGCCGA+GGG - tig0041264:725-744 Msa1461080:intron 45.0%
AAATGTCTCAGTCTCACCCT+CGG + tig0041264:744-763 None:intergenic 45.0%
GCTATTGCTTCTGGTAAAGG+CGG - tig0041264:909-928 Msa1461080:intron 45.0%
!! CATCAAAGTTGATGAGGCCG+AGG - tig0041264:724-743 Msa1461080:intron 50.0%
! GCGAAGCATCTTCGCATTGA+TGG - tig0041264:870-889 Msa1461080:intron 50.0%
GTCGGCAAATCAACAACTGC+TGG - tig0041264:933-952 Msa1461080:intron 50.0%
!! CATCAAAGTTGATGAGGCCG+AGG - tig0041264:724-743 Msa1461080:intron 50.0%
! GCGAAGCATCTTCGCATTGA+TGG - tig0041264:870-889 Msa1461080:intron 50.0%
GTCGGCAAATCAACAACTGC+TGG - tig0041264:933-952 Msa1461080:intron 50.0%
GTGATGTCGGGGGAAAATCG+TGG + tig0041264:675-694 None:intergenic 55.0%
GCTTCTGGTAAAGGCGGTGT+CGG - tig0041264:915-934 Msa1461080:intron 55.0%
GTGATGTCGGGGGAAAATCG+TGG + tig0041264:675-694 None:intergenic 55.0%
GCTTCTGGTAAAGGCGGTGT+CGG - tig0041264:915-934 Msa1461080:intron 55.0%
Chromosome Type Strat End Strand Name
tig0041264 gene 519 2057 519 ID=Msa1461080;Name=Msa1461080
tig0041264 mRNA 519 2057 519 ID=Msa1461080-mRNA-1;Parent=Msa1461080;Name=Msa1461080-mRNA-1;_AED=0.00;_eAED=0.00;_QI=216|1|0.66|1|1|1|3|0|81
tig0041264 exon 519 640 519 ID=Msa1461080-mRNA-1:exon:23017;Parent=Msa1461080-mRNA-1
tig0041264 exon 1622 1730 1622 ID=Msa1461080-mRNA-1:exon:23016;Parent=Msa1461080-mRNA-1
tig0041264 exon 1830 2057 1830 ID=Msa1461080-mRNA-1:exon:23015;Parent=Msa1461080-mRNA-1
tig0041264 five_prime_UTR 1842 2057 1842 ID=Msa1461080-mRNA-1:five_prime_utr;Parent=Msa1461080-mRNA-1
tig0041264 CDS 1830 1841 1830 ID=Msa1461080-mRNA-1:cds;Parent=Msa1461080-mRNA-1
tig0041264 CDS 1622 1730 1622 ID=Msa1461080-mRNA-1:cds;Parent=Msa1461080-mRNA-1
tig0041264 CDS 519 640 519 ID=Msa1461080-mRNA-1:cds;Parent=Msa1461080-mRNA-1
Gene Sequence

>Msa1461080

ATGAGGCCGAGGTGGCTAGGAAGTGTTAGAAGCTACGCGAAGCATCTTCGCATTGATGGAGTCAAAGATACCATAGCTATTGCTTCTGGTAAAGGCGGTGTCGGCAAATCAACAACTGCTGTGAATTTAGCTGTTGCGCTTGCAAGTAAGTTCCAATTGAAGGTTGGGTTACTTGATGCTGATGTGTATGGACCCAACATTCCTATCATGATGAACATCGACACAAAGCCTGAAGTCACTCAA

Protein sequence

>Msa1461080

MRPRWLGSVRSYAKHLRIDGVKDTIAIASGKGGVGKSTTAVNLAVALASKFQLKVGLLDADVYGPNIPIMMNIDTKPEVTQ