AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1465450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465450 MtrunA17_Chr1g0186331 95.146 103 5 0 1 103 1 103 4.50e-60 179
Msa1465450 MtrunA17_Chr1g0168521 49.038 104 45 3 4 103 2 101 2.88e-20 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465450 AT2G33390.1 41.346 104 53 4 2 103 1 98 9.18e-14 62.0
Msa1465450 AT2G33390.2 37.634 93 52 3 2 94 1 87 6.64e-11 54.7

Find 65 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTTGCCTTCTTCTTAT+TGG 0.184049 tig0045253:-4425 Msa1465450:five_prime_UTR
GCCTGTGGATGTGGCTTCTT+TGG 0.209800 tig0045253:-4328 Msa1465450:CDS
TAAGTAACTAAGAAATTTAA+AGG 0.231394 tig0045253:+1755 None:intergenic
ATGAATCGAAGGAACATTAT+TGG 0.284730 tig0045253:-2065 Msa1465450:three_prime_UTR
GGCTTCTTTGGATTGTAAAA+AGG 0.314586 tig0045253:-4316 Msa1465450:CDS
TGAATCGAAGGAACATTATT+GGG 0.325430 tig0045253:-2064 Msa1465450:three_prime_UTR
TTTATATTTACTTCAAATTC+GGG 0.330007 tig0045253:+2032 None:intergenic
AAAGTTTGAGACAGGTTATC+AGG 0.371142 tig0045253:+2095 None:intergenic
TTGTTACTTGTTGTTGATGA+TGG 0.374801 tig0045253:-4397 Msa1465450:exon
CAGTTTGTGCTCCTGGTCCC+TGG 0.385510 tig0045253:-1660 Msa1465450:three_prime_UTR
GATGAACTAGATCCTTATCT+TGG 0.397484 tig0045253:+1994 None:intergenic
ACTGTCCAAATCAAAATTGA+TGG 0.415555 tig0045253:+1898 None:intergenic
GTTATGTCAGTTTGTGCTCC+TGG 0.421767 tig0045253:-1667 Msa1465450:three_prime_UTR
ATATTATACATTCAATTGCT+TGG 0.424329 tig0045253:-1820 Msa1465450:three_prime_UTR
GAGTGTCGTCAACAAAGAAT+TGG 0.429184 tig0045253:+4251 None:intergenic
GGATGATACTGTGACCAAAA+AGG 0.437333 tig0045253:-4376 Msa1465450:CDS
TACTTCAAATTCGGGATCTA+CGG 0.445633 tig0045253:+2040 None:intergenic
ATCAATTCAGATTCTTGTTG+TGG 0.454464 tig0045253:-4617 Msa1465450:five_prime_UTR
AGGAGGAATGGCTCAAAAGT+TGG 0.455498 tig0045253:-4220 Msa1465450:CDS
TTCAGTAGAAAGTTTGAGAC+AGG 0.456310 tig0045253:+2087 None:intergenic
TGTGAATAAGCCTGTGGATG+TGG 0.459711 tig0045253:-4337 Msa1465450:CDS
TAGATCCTTATCTTGGCTGT+TGG 0.472547 tig0045253:+2001 None:intergenic
TGTTGCAGCTATGCAATTTG+AGG 0.478704 tig0045253:+1512 Msa1465440:three_prime_UTR
TTGAGACAGAACAGAACAAA+AGG 0.485586 tig0045253:+4671 None:intergenic
GAAGATCATGGTCATGTCAA+TGG 0.485595 tig0045253:-4291 Msa1465450:CDS
GTTCATGTTGTAAATGAAAG+AGG 0.488811 tig0045253:-1798 Msa1465450:three_prime_UTR
TACAATTGTATACAAACTCC+AGG 0.490301 tig0045253:+1642 None:intergenic
CAAGAATCTGAATTGATTCA+CGG 0.491740 tig0045253:+4623 None:intergenic
CAGATGATGGAGAATTTGTA+TGG 0.491801 tig0045253:+4569 None:intergenic
CGCAATAAATTGTCAAAACC+AGG 0.498420 tig0045253:+1968 None:intergenic
TGATGGAGAATTTGTATGGT+TGG 0.504663 tig0045253:+4573 None:intergenic
AAACAAGCTTGTTGAGGTGT+TGG 0.509553 tig0045253:-4157 Msa1465450:CDS
ATTCTTAAATTTAGATCCTG+TGG 0.525744 tig0045253:-4732 Msa1465450:five_prime_UTR
TCCAAAGAAGCCACATCCAC+AGG 0.532777 tig0045253:+4327 None:intergenic
AATGAAGATGAAGCAGATGA+TGG 0.533758 tig0045253:+4556 None:intergenic
TTGACGACACTCCCTAAAAG+AGG 0.533850 tig0045253:-4240 Msa1465450:CDS
AGGATCTAGTTCATCTAACC+TGG 0.533947 tig0045253:-1986 Msa1465450:three_prime_UTR
CAACACTTCCAATAAGAAGA+AGG 0.538014 tig0045253:+4417 None:intergenic
ACTCTCCAACAGCCAAGATA+AGG 0.539774 tig0045253:-2006 Msa1465450:three_prime_UTR
CTCAACAAGCTTGTTTCCAA+TGG 0.552684 tig0045253:+4164 None:intergenic
CTTTCTACTGAATGAATCGA+AGG 0.558989 tig0045253:-2076 Msa1465450:three_prime_UTR
AAAAGTTGGATAAGACATGT+AGG 0.571842 tig0045253:-4206 Msa1465450:CDS
TGATGTTATGTGAAGTGTGT+GGG 0.573174 tig0045253:+4806 None:intergenic
AAGGTTGTGAAATAATAAGA+CGG 0.577396 tig0045253:+4690 None:intergenic
GTATACAAACTCCAGGGACC+AGG 0.579980 tig0045253:+1649 None:intergenic
AGGTTGTGAAATAATAAGAC+GGG 0.581553 tig0045253:+4691 None:intergenic
ACTCCCTAAAAGAGGAGGAA+TGG 0.586268 tig0045253:-4232 Msa1465450:CDS
CTATGCAATTTGAGGTGTTG+AGG 0.586708 tig0045253:+1520 None:intergenic
GTGCAGTGGACTGATACCAT+TGG 0.587155 tig0045253:-4180 Msa1465450:CDS
GGACAGTGCTGATTTGAGCA+CGG 0.596400 tig0045253:-1882 Msa1465450:three_prime_UTR
TCTCAAATCTTATGCTCACA+AGG 0.600946 tig0045253:+1546 None:intergenic
GTAGACAATCTAAGCAACAT+AGG 0.603646 tig0045253:+1601 None:intergenic
CATTGGAAACAAGCTTGTTG+AGG 0.604618 tig0045253:-4163 Msa1465450:CDS
CGGAAGAATATCACTACACA+CGG 0.605058 tig0045253:+4643 None:intergenic
ATGATGTTATGTGAAGTGTG+TGG 0.623270 tig0045253:+4805 None:intergenic
AGAATACAACAAAACAAACA+AGG 0.636499 tig0045253:+4772 None:intergenic
GAATCGAAGGAACATTATTG+GGG 0.645264 tig0045253:-2063 Msa1465450:three_prime_UTR
GAATACAACAAAACAAACAA+GGG 0.659390 tig0045253:+4773 None:intergenic
TGTTAGTGACTCAGATGATG+AGG 0.670468 tig0045253:-2151 Msa1465450:CDS
TGGCTCTGTGAATAAGCCTG+TGG 0.674534 tig0045253:-4343 Msa1465450:CDS
ACAATTGTATACAAACTCCA+GGG 0.678972 tig0045253:+1643 None:intergenic
ATGGGTTGAAATCAAAACAG+AGG 0.679889 tig0045253:+4511 None:intergenic
ACGACACTCCCTAAAAGAGG+AGG 0.681962 tig0045253:-4237 Msa1465450:CDS
AGGTGTTGGAATATGAACCA+AGG 0.696830 tig0045253:-4143 Msa1465450:intron
AGACATGTAGGAGAGTGCAG+TGG 0.721899 tig0045253:-4194 Msa1465450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATATTTACTTCAAATT+CGG + tig0045253:4255-4274 None:intergenic 10.0%
!! AAAAAAAATCAGAAAAATCA+AGG + tig0045253:1836-1855 None:intergenic 15.0%
!!! TGTTATTTTAATGTTACATA+TGG - tig0045253:2460-2479 Msa1465450:intron 15.0%
!! ATAATACTAGTTAAATTGAA+GGG - tig0045253:3304-3323 Msa1465450:intron 15.0%
!! TTTATATTTACTTCAAATTC+GGG + tig0045253:4254-4273 None:intergenic 15.0%
!! TAAGTAACTAAGAAATTTAA+AGG + tig0045253:4531-4550 None:intergenic 15.0%
!! AAAAAATAAAAATTCACCAC+AGG + tig0045253:1570-1589 None:intergenic 20.0%
!! TAACTGATAAAAATTTACCT+TGG + tig0045253:2160-2179 None:intergenic 20.0%
!!! TGGTATTTTGGTTAAATTTA+TGG - tig0045253:2411-2430 Msa1465450:intron 20.0%
!!! TTTTAATGTTACATATGGAA+TGG - tig0045253:2465-2484 Msa1465450:intron 20.0%
!!! TTTTTCAAAAACACTAGATT+TGG + tig0045253:2807-2826 None:intergenic 20.0%
!!! TTAAACAGTTCAGATTTTAA+TGG + tig0045253:3091-3110 None:intergenic 20.0%
!! TTGCAAATGTTAAATATTAC+TGG - tig0045253:3203-3222 Msa1465450:intron 20.0%
!! GATAATACTAGTTAAATTGA+AGG - tig0045253:3303-3322 Msa1465450:intron 20.0%
!!! TCACAAGTTTATAGTTTTAA+TGG + tig0045253:3475-3494 None:intergenic 20.0%
!!! TTTTAATTGTTCATCGTTTA+TGG - tig0045253:3556-3575 Msa1465450:intron 20.0%
!! AATAACTGAATTGTAGTAAA+GGG + tig0045253:3650-3669 None:intergenic 20.0%
!! AAATAACTGAATTGTAGTAA+AGG + tig0045253:3651-3670 None:intergenic 20.0%
!! ATATTATACATTCAATTGCT+TGG - tig0045253:4463-4482 Msa1465450:five_prime_UTR 20.0%
! GAATACAACAAAACAAACAA+GGG + tig0045253:1513-1532 None:intergenic 25.0%
! AGAATACAACAAAACAAACA+AGG + tig0045253:1514-1533 None:intergenic 25.0%
! ATTCTTAAATTTAGATCCTG+TGG - tig0045253:1551-1570 Msa1465450:three_prime_UTR 25.0%
! AAGGTTGTGAAATAATAAGA+CGG + tig0045253:1596-1615 None:intergenic 25.0%
!!! ATCCAAAAATCACTGATTTT+TGG + tig0045253:1810-1829 None:intergenic 25.0%
! TTCACTATTTGTACAAAACA+AGG + tig0045253:2383-2402 None:intergenic 25.0%
! ACAAATAGTGAAATTTGAGA+TGG - tig0045253:2391-2410 Msa1465450:intron 25.0%
!!! TGAAATTTGAGATGGTATTT+TGG - tig0045253:2399-2418 Msa1465450:intron 25.0%
! AGAATATGAAAATGAGTTTG+TGG - tig0045253:2615-2634 Msa1465450:intron 25.0%
! GAATATGAAAATGAGTTTGT+GGG - tig0045253:2616-2635 Msa1465450:intron 25.0%
! GGTTAAGATTAAAAACCTTT+GGG - tig0045253:2731-2750 Msa1465450:intron 25.0%
! TCTAAGTTAGACATATTTAG+AGG - tig0045253:2951-2970 Msa1465450:intron 25.0%
! TGAAATACCATCTTAAAATC+TGG + tig0045253:3048-3067 None:intergenic 25.0%
! ATATTACTGGAATACATCTT+TGG - tig0045253:3216-3235 Msa1465450:intron 25.0%
! TAAATTGAAGGGAGATTTAT+TGG - tig0045253:3315-3334 Msa1465450:intron 25.0%
! ATGTCATAAAGATAAGTGTT+TGG - tig0045253:3363-3382 Msa1465450:intron 25.0%
! TTCAGTTATTACATAGAAAC+TGG - tig0045253:3397-3416 Msa1465450:intron 25.0%
! CTACAATTCAGTTATTTAGA+CGG - tig0045253:3654-3673 Msa1465450:intron 25.0%
!!! TTCGGTTTTTAAAACATTGA+TGG - tig0045253:3751-3770 Msa1465450:intron 25.0%
!!! TCGGTTTTTAAAACATTGAT+GGG - tig0045253:3752-3771 Msa1465450:intron 25.0%
! TAACTAGATGTATAATGCAA+AGG - tig0045253:3856-3875 Msa1465450:intron 25.0%
!!! ACACTTTTTGACATATCATA+AGG + tig0045253:4036-4055 None:intergenic 25.0%
! ATCCATTATTATCTGACATT+TGG - tig0045253:4086-4105 Msa1465450:intron 25.0%
! TTTAAAGGAAAAAAAGACTG+AGG + tig0045253:4516-4535 None:intergenic 25.0%
AGGTTGTGAAATAATAAGAC+GGG + tig0045253:1595-1614 None:intergenic 30.0%
! CAAGAATCTGAATTGATTCA+CGG + tig0045253:1663-1682 None:intergenic 30.0%
ATCAATTCAGATTCTTGTTG+TGG - tig0045253:1666-1685 Msa1465450:three_prime_UTR 30.0%
ATCAAAACAGAGGAAAAACA+GGG + tig0045253:1765-1784 None:intergenic 30.0%
AATCAAAACAGAGGAAAAAC+AGG + tig0045253:1766-1785 None:intergenic 30.0%
!! TTTTGGAGGAATGAATAGAT+GGG + tig0045253:1793-1812 None:intergenic 30.0%
!! TTTTTGGAGGAATGAATAGA+TGG + tig0045253:1794-1813 None:intergenic 30.0%
!!! CAAAAATCACTGATTTTTGG+AGG + tig0045253:1807-1826 None:intergenic 30.0%
! CTCCAAAAATCAGTGATTTT+TGG - tig0045253:1805-1824 Msa1465450:three_prime_UTR 30.0%
TGAATTTGCCTTCTTCTTAT+TGG - tig0045253:1858-1877 Msa1465450:three_prime_UTR 30.0%
!! TTGTTACTTGTTGTTGATGA+TGG - tig0045253:1886-1905 Msa1465450:three_prime_UTR 30.0%
ACCAAAAAGGATACTGTTAA+TGG - tig0045253:1920-1939 Msa1465450:three_prime_UTR 30.0%
TGTAAAAAGGTTGAAGATCA+TGG - tig0045253:1980-1999 Msa1465450:three_prime_UTR 30.0%
AAAAGTTGGATAAGACATGT+AGG - tig0045253:2077-2096 Msa1465450:three_prime_UTR 30.0%
! ACTATGGTTCAAATGAGTTT+TGG + tig0045253:2313-2332 None:intergenic 30.0%
TGTGTGTATACTAGGAAAAA+AGG - tig0045253:2332-2351 Msa1465450:intron 30.0%
AGGAAAAAAGGAGTTGAAAT+TGG - tig0045253:2344-2363 Msa1465450:intron 30.0%
TACATATGGAATGGAATCTT+TGG - tig0045253:2474-2493 Msa1465450:intron 30.0%
GAATGGAATCTTTGGTAAAT+AGG - tig0045253:2482-2501 Msa1465450:intron 30.0%
ATTTCAGAGATAACGAATTC+GGG + tig0045253:2561-2580 None:intergenic 30.0%
GATTTCAGAGATAACGAATT+CGG + tig0045253:2562-2581 None:intergenic 30.0%
!! AATTCGTTATCTCTGAAATC+CGG - tig0045253:2562-2581 Msa1465450:intron 30.0%
! ATAGGATTTTGCCTAAATGT+AGG + tig0045253:2677-2696 None:intergenic 30.0%
CATTTAGGCAAAATCCTATT+AGG - tig0045253:2678-2697 Msa1465450:intron 30.0%
AATCCTATTAGGAGATGATT+TGG - tig0045253:2689-2708 Msa1465450:intron 30.0%
! TGATTTGGAGTCAAATTTGT+AGG - tig0045253:2704-2723 Msa1465450:intron 30.0%
! GATTTGGAGTCAAATTTGTA+GGG - tig0045253:2705-2724 Msa1465450:intron 30.0%
CGGTTAAGATTAAAAACCTT+TGG - tig0045253:2730-2749 Msa1465450:intron 30.0%
! TACTGGAACTTCATTCATTT+TGG - tig0045253:2769-2788 Msa1465450:intron 30.0%
TATGTCTAACTTAGACGAAT+GGG + tig0045253:2946-2965 None:intergenic 30.0%
ATATGTCTAACTTAGACGAA+TGG + tig0045253:2947-2966 None:intergenic 30.0%
ATTTGCAAATGTTAATCCTC+AGG + tig0045253:3191-3210 None:intergenic 30.0%
TAATACAGATAAGCTCTTAG+TGG - tig0045253:3266-3285 Msa1465450:intron 30.0%
!!! TATAGTTTTAATGGAGCTAC+AGG + tig0045253:3466-3485 None:intergenic 30.0%
TGTGATATGATAGAAACTGT+TGG - tig0045253:3490-3509 Msa1465450:intron 30.0%
GTGATATGATAGAAACTGTT+GGG - tig0045253:3491-3510 Msa1465450:intron 30.0%
TGATATGATAGAAACTGTTG+GGG - tig0045253:3492-3511 Msa1465450:intron 30.0%
!!! CACTGTTTTAAAAACCAAAC+TGG - tig0045253:3588-3607 Msa1465450:intron 30.0%
!!! GTTTTAAAAACCAAACTGGA+TGG - tig0045253:3592-3611 Msa1465450:intron 30.0%
TTATTTAGACGGTTCAATTG+TGG - tig0045253:3665-3684 Msa1465450:intron 30.0%
ATGCAAAGGTGAAAATTGAT+AGG - tig0045253:3870-3889 Msa1465450:intron 30.0%
GACCAAATGTCAGATAATAA+TGG + tig0045253:4091-4110 None:intergenic 30.0%
ATGAATCGAAGGAACATTAT+TGG - tig0045253:4218-4237 Msa1465450:CDS 30.0%
TGAATCGAAGGAACATTATT+GGG - tig0045253:4219-4238 Msa1465450:CDS 30.0%
!!! GACATCCATCAATTTTGATT+TGG - tig0045253:4380-4399 Msa1465450:CDS 30.0%
ACTGTCCAAATCAAAATTGA+TGG + tig0045253:4388-4407 None:intergenic 30.0%
GTTCATGTTGTAAATGAAAG+AGG - tig0045253:4485-4504 Msa1465450:five_prime_UTR 30.0%
ACAATTGTATACAAACTCCA+GGG + tig0045253:4643-4662 None:intergenic 30.0%
TACAATTGTATACAAACTCC+AGG + tig0045253:4644-4663 None:intergenic 30.0%
TTGAGACAGAACAGAACAAA+AGG + tig0045253:1615-1634 None:intergenic 35.0%
TGATGGAGAATTTGTATGGT+TGG + tig0045253:1713-1732 None:intergenic 35.0%
!! CAGATGATGGAGAATTTGTA+TGG + tig0045253:1717-1736 None:intergenic 35.0%
AATGAAGATGAAGCAGATGA+TGG + tig0045253:1730-1749 None:intergenic 35.0%
TCAAAACAGAGGAAAAACAG+GGG + tig0045253:1764-1783 None:intergenic 35.0%
! ATGGGTTGAAATCAAAACAG+AGG + tig0045253:1775-1794 None:intergenic 35.0%
CAACACTTCCAATAAGAAGA+AGG + tig0045253:1869-1888 None:intergenic 35.0%
! GCCATTAACAGTATCCTTTT+TGG + tig0045253:1924-1943 None:intergenic 35.0%
GGCTTCTTTGGATTGTAAAA+AGG - tig0045253:1967-1986 Msa1465450:three_prime_UTR 35.0%
AACCATAGTGTGTGTATACT+AGG - tig0045253:2324-2343 Msa1465450:intron 35.0%
TTCCTAGTATACACACACTA+TGG + tig0045253:2329-2348 None:intergenic 35.0%
GAAAATGAGTTTGTGGGATT+TGG - tig0045253:2622-2641 Msa1465450:intron 35.0%
! TGTAGGAACTTCAAGGATTT+TGG + tig0045253:2660-2679 None:intergenic 35.0%
TCCTTGAAGTTCCTACATTT+AGG - tig0045253:2663-2682 Msa1465450:intron 35.0%
ACTCCAAATCATCTCCTAAT+AGG + tig0045253:2695-2714 None:intergenic 35.0%
ACAACATAAGTCTGTCTGAA+TGG - tig0045253:2826-2845 Msa1465450:intron 35.0%
!! GAGGAATTTGTATTTGCTGT+AGG - tig0045253:2970-2989 Msa1465450:intron 35.0%
!! TGTCTGTCCAGATTTTAAGA+TGG - tig0045253:3038-3057 Msa1465450:intron 35.0%
!! AATCCAAATTTTAGCACCTG+AGG - tig0045253:3172-3191 Msa1465450:intron 35.0%
AATCCTCAGGTGCTAAAATT+TGG + tig0045253:3178-3197 None:intergenic 35.0%
ATTGTTCATCGTTTATGGCA+TGG - tig0045253:3561-3580 Msa1465450:intron 35.0%
GAATTGTAGTAAAGGGCTAA+AGG + tig0045253:3643-3662 None:intergenic 35.0%
! CTCAATTCGAGTAGTTGATA+TGG - tig0045253:3694-3713 Msa1465450:intron 35.0%
GATCTCACAAGTTTGATGTT+AGG - tig0045253:3728-3747 Msa1465450:intron 35.0%
CACAAGTTTGATGTTAGGTT+CGG - tig0045253:3733-3752 Msa1465450:intron 35.0%
AGAACCAAAAAGTTATGCAC+TGG + tig0045253:3813-3832 None:intergenic 35.0%
ATGTATAATGCTGGAAGAGA+AGG - tig0045253:3903-3922 Msa1465450:intron 35.0%
! AAAGTTTGAGACAGGTTATC+AGG + tig0045253:4191-4210 None:intergenic 35.0%
! TTCAGTAGAAAGTTTGAGAC+AGG + tig0045253:4199-4218 None:intergenic 35.0%
CTTTCTACTGAATGAATCGA+AGG - tig0045253:4207-4226 Msa1465450:CDS 35.0%
GAATCGAAGGAACATTATTG+GGG - tig0045253:4220-4239 Msa1465450:CDS 35.0%
TACTTCAAATTCGGGATCTA+CGG + tig0045253:4246-4265 None:intergenic 35.0%
GATGAACTAGATCCTTATCT+TGG + tig0045253:4292-4311 None:intergenic 35.0%
CGCAATAAATTGTCAAAACC+AGG + tig0045253:4318-4337 None:intergenic 35.0%
!! GGTTTTGACAATTTATTGCG+TGG - tig0045253:4318-4337 Msa1465450:CDS 35.0%
! ATTTTGCTACAAGGGTTCTT+TGG - tig0045253:4594-4613 Msa1465450:five_prime_UTR 35.0%
GTAGACAATCTAAGCAACAT+AGG + tig0045253:4685-4704 None:intergenic 35.0%
TCTCAAATCTTATGCTCACA+AGG + tig0045253:4740-4759 None:intergenic 35.0%
CGGAAGAATATCACTACACA+CGG + tig0045253:1643-1662 None:intergenic 40.0%
GGATGATACTGTGACCAAAA+AGG - tig0045253:1907-1926 Msa1465450:three_prime_UTR 40.0%
GAAGATCATGGTCATGTCAA+TGG - tig0045253:1992-2011 Msa1465450:three_prime_UTR 40.0%
! GAGTGTCGTCAACAAAGAAT+TGG + tig0045253:2035-2054 None:intergenic 40.0%
! CTCAACAAGCTTGTTTCCAA+TGG + tig0045253:2122-2141 None:intergenic 40.0%
!! CATTGGAAACAAGCTTGTTG+AGG - tig0045253:2120-2139 Msa1465450:exon 40.0%
!! AAACAAGCTTGTTGAGGTGT+TGG - tig0045253:2126-2145 Msa1465450:CDS 40.0%
!! AGGTGTTGGAATATGAACCA+AGG - tig0045253:2140-2159 Msa1465450:CDS 40.0%
GCCTAAATGTAGGAACTTCA+AGG + tig0045253:2667-2686 None:intergenic 40.0%
!! ACCTTTGGGATCTGTCTAAA+TGG - tig0045253:2745-2764 Msa1465450:intron 40.0%
TTGCGTATCCAGTAGTTCTT+TGG - tig0045253:2915-2934 Msa1465450:intron 40.0%
TTTGGCCGATAATACAGAGA+TGG - tig0045253:3234-3253 Msa1465450:intron 40.0%
TTGGCCGATAATACAGAGAT+GGG - tig0045253:3235-3254 Msa1465450:intron 40.0%
!! AAGCTCTTAGTGGATTCTGA+AGG - tig0045253:3276-3295 Msa1465450:intron 40.0%
TGATAGAAACTGTTGGGGAA+AGG - tig0045253:3497-3516 Msa1465450:intron 40.0%
GATAGAAACTGTTGGGGAAA+GGG - tig0045253:3498-3517 Msa1465450:intron 40.0%
AGAGCAGAAACTAGCACAAT+AGG - tig0045253:3521-3540 Msa1465450:intron 40.0%
! GTGACCAGTGCATAACTTTT+TGG - tig0045253:3806-3825 Msa1465450:intron 40.0%
GCTGCTTCTATGTATAATGC+TGG - tig0045253:3894-3913 Msa1465450:intron 40.0%
CAATGCTTTCTTGCCTACTT+GGG - tig0045253:3946-3965 Msa1465450:intron 40.0%
AATGCTTTCTTGCCTACTTG+GGG - tig0045253:3947-3966 Msa1465450:intron 40.0%
ACATCACTGCACACACAATT+TGG + tig0045253:4115-4134 None:intergenic 40.0%
TGTTAGTGACTCAGATGATG+AGG - tig0045253:4132-4151 Msa1465450:intron 40.0%
TAGATCCTTATCTTGGCTGT+TGG + tig0045253:4285-4304 None:intergenic 40.0%
! AGGATCTAGTTCATCTAACC+TGG - tig0045253:4297-4316 Msa1465450:CDS 40.0%
! ACTCGTGCAATTTTGCTACA+AGG - tig0045253:4585-4604 Msa1465450:five_prime_UTR 40.0%
! CTCGTGCAATTTTGCTACAA+GGG - tig0045253:4586-4605 Msa1465450:five_prime_UTR 40.0%
!! CTATGCAATTTGAGGTGTTG+AGG + tig0045253:4766-4785 None:intergenic 40.0%
TGTTGCAGCTATGCAATTTG+AGG + tig0045253:4774-4793 None:intergenic 40.0%
TGTGAATAAGCCTGTGGATG+TGG - tig0045253:1946-1965 Msa1465450:three_prime_UTR 45.0%
TTGACGACACTCCCTAAAAG+AGG - tig0045253:2043-2062 Msa1465450:three_prime_UTR 45.0%
ACTCCCTAAAAGAGGAGGAA+TGG - tig0045253:2051-2070 Msa1465450:three_prime_UTR 45.0%
! GAGCCATTCCTCCTCTTTTA+GGG + tig0045253:2057-2076 None:intergenic 45.0%
! TGAGCCATTCCTCCTCTTTT+AGG + tig0045253:2058-2077 None:intergenic 45.0%
AGGAGGAATGGCTCAAAAGT+TGG - tig0045253:2063-2082 Msa1465450:three_prime_UTR 45.0%
CTGAAATCCGGAGTGATTCT+TGG - tig0045253:2574-2593 Msa1465450:intron 45.0%
ATTCTAACCAGAGCTTCCCT+CGG + tig0045253:2600-2619 None:intergenic 45.0%
GGAGTCAAATTTGTAGGGTG+CGG - tig0045253:2710-2729 Msa1465450:intron 45.0%
GCCATTTAGACAGATCCCAA+AGG + tig0045253:2749-2768 None:intergenic 45.0%
GGATCTGTCTAAATGGCTAC+TGG - tig0045253:2752-2771 Msa1465450:intron 45.0%
AGCATGAATGACGAACTGAC+TGG - tig0045253:2857-2876 Msa1465450:intron 45.0%
GCATGAATGACGAACTGACT+GGG - tig0045253:2858-2877 Msa1465450:intron 45.0%
GGGTGTTTCCAAAGAACTAC+TGG + tig0045253:2926-2945 None:intergenic 45.0%
CCCTCCCATCTCTGTATTAT+CGG + tig0045253:3242-3261 None:intergenic 45.0%
CCGATAATACAGAGATGGGA+GGG - tig0045253:3239-3258 Msa1465450:intron 45.0%
GCAGAAACTAGCACAATAGG+TGG - tig0045253:3524-3543 Msa1465450:intron 45.0%
ACTGGTTGAACCATCCAGTT+TGG + tig0045253:3605-3624 None:intergenic 45.0%
TGTAGTAAAGGGCTAAAGGC+TGG + tig0045253:3639-3658 None:intergenic 45.0%
CCTAAGTCTGGTAGCTTCTA+GGG + tig0045253:3786-3805 None:intergenic 45.0%
CCTAGAAGCTACCAGACTTA+GGG - tig0045253:3784-3803 Msa1465450:intron 45.0%
! CCAAGTAGGCAAGAAAGCAT+TGG + tig0045253:3948-3967 None:intergenic 45.0%
CCAATGCTTTCTTGCCTACT+TGG - tig0045253:3945-3964 Msa1465450:intron 45.0%
TGATACAGTGTACCCCAAGT+AGG + tig0045253:3962-3981 None:intergenic 45.0%
ACTCTCCAACAGCCAAGATA+AGG - tig0045253:4277-4296 Msa1465450:CDS 45.0%
GTTATGTCAGTTTGTGCTCC+TGG - tig0045253:4616-4635 Msa1465450:five_prime_UTR 45.0%
! TGGCTCTGTGAATAAGCCTG+TGG - tig0045253:1940-1959 Msa1465450:three_prime_UTR 50.0%
TCCAAAGAAGCCACATCCAC+AGG + tig0045253:1959-1978 None:intergenic 50.0%
ACGACACTCCCTAAAAGAGG+AGG - tig0045253:2046-2065 Msa1465450:three_prime_UTR 50.0%
! AGACATGTAGGAGAGTGCAG+TGG - tig0045253:2089-2108 Msa1465450:three_prime_UTR 50.0%
!! GTGCAGTGGACTGATACCAT+TGG - tig0045253:2103-2122 Msa1465450:three_prime_UTR 50.0%
TTCACGCAGTCGCACATTGT+TGG - tig0045253:3006-3025 Msa1465450:intron 50.0%
TGTCTGACTGAGTGAATGCG+TGG - tig0045253:3132-3151 Msa1465450:intron 50.0%
GCCGATAATACAGAGATGGG+AGG - tig0045253:3238-3257 Msa1465450:intron 50.0%
! AGGCTGGTTCATAGTTCGAC+TGG + tig0045253:3623-3642 None:intergenic 50.0%
CCCTAGAAGCTACCAGACTT+AGG - tig0045253:3783-3802 Msa1465450:intron 50.0%
CCCTAAGTCTGGTAGCTTCT+AGG + tig0045253:3787-3806 None:intergenic 50.0%
!! GGACAGTGCTGATTTGAGCA+CGG - tig0045253:4401-4420 Msa1465450:exon 50.0%
GTATACAAACTCCAGGGACC+AGG + tig0045253:4637-4656 None:intergenic 50.0%
GCCTGTGGATGTGGCTTCTT+TGG - tig0045253:1955-1974 Msa1465450:three_prime_UTR 55.0%
GTGTCAGTGACAGTGTGTGC+TGG - tig0045253:2238-2257 Msa1465450:intron 55.0%
! TGCACTGGTCACCCTAAGTC+TGG + tig0045253:3798-3817 None:intergenic 55.0%
TCCGGAGTGATTCTTGGCCG+AGG - tig0045253:2580-2599 Msa1465450:intron 60.0%
CCCTCGGCCAAGAATCACTC+CGG + tig0045253:2584-2603 None:intergenic 60.0%
CCGGAGTGATTCTTGGCCGA+GGG - tig0045253:2581-2600 Msa1465450:intron 60.0%
TTCTTGGCCGAGGGAAGCTC+TGG - tig0045253:2590-2609 Msa1465450:intron 60.0%
CAGTTTGTGCTCCTGGTCCC+TGG - tig0045253:4623-4642 Msa1465450:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
tig0045253 gene 1480 4825 1480 ID=Msa1465450;Name=Msa1465450
tig0045253 mRNA 1480 4825 1480 ID=Msa1465450-mRNA-1;Parent=Msa1465450;Name=Msa1465450-mRNA-1;_AED=0.00;_eAED=0.00;_QI=422|1|1|1|1|1|2|645|103
tig0045253 exon 1480 2176 1480 ID=Msa1465450-mRNA-1:exon:24800;Parent=Msa1465450-mRNA-1
tig0045253 exon 4144 4825 4144 ID=Msa1465450-mRNA-1:exon:24799;Parent=Msa1465450-mRNA-1
tig0045253 five_prime_UTR 4404 4825 4404 ID=Msa1465450-mRNA-1:five_prime_utr;Parent=Msa1465450-mRNA-1
tig0045253 CDS 4144 4403 4144 ID=Msa1465450-mRNA-1:cds;Parent=Msa1465450-mRNA-1
tig0045253 CDS 2125 2176 2125 ID=Msa1465450-mRNA-1:cds;Parent=Msa1465450-mRNA-1
tig0045253 three_prime_UTR 1480 2124 1480 ID=Msa1465450-mRNA-1:three_prime_utr;Parent=Msa1465450-mRNA-1
Gene Sequence

>Msa1465450

ATGATGGATGATACTGTGACCAAAAAGGATACTGTTAATGGCTCTGTGAATAAGCCTGTGGATGTGGCTTCTTTGGATTGTAAAAAGGTTGAAGATCATGGTCATGTCAATGGAAAAAATGATAGTGATTCCAATTCTTTGTTGACGACACTCCCTAAAAGAGGAGGAATGGCTCAAAAGTTGGATAAGACATGTAGGAGAGTGCAGTGGACTGATACCATTGGAAACAAGCTTGTTGAGGTGTTGGAATATGAACCAAGTGATGTTAGTGACTCAGATGATGAGGATGCTTGTATCTGCTCAATAATGTAG
Protein sequence

>Msa1465450

MMDDTVTKKDTVNGSVNKPVDVASLDCKKVEDHGHVNGKNDSDSNSLLTTLPKRGGMAQKLDKTCRRVQWTDTIGNKLVEVLEYEPSDVSDSDDEDACICSIM*