AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000081

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000081.t1 MTR_2g438440 82.332 566 98 2 3 568 141 704 0.0 953
MS.gene000081.t1 MTR_2g438700 80.423 567 108 3 3 568 144 708 0.0 932
MS.gene000081.t1 MTR_2g438720 79.718 567 112 3 3 568 146 710 0.0 920
MS.gene000081.t1 MTR_2g438400 79.893 562 111 2 7 568 4 563 0.0 918
MS.gene000081.t1 MTR_2g438640 77.562 566 125 2 3 568 146 709 0.0 894
MS.gene000081.t1 MTR_2g438640 75.088 566 113 3 3 568 146 683 0.0 848
MS.gene000081.t1 MTR_2g438470 76.393 377 53 3 60 436 158 498 0.0 568
MS.gene000081.t1 MTR_2g438740 50.987 557 263 5 8 563 33 580 0.0 561
MS.gene000081.t1 MTR_2g438760 47.935 557 269 6 8 563 39 575 1.22e-173 504
MS.gene000081.t1 MTR_2g438640 73.152 257 68 1 3 259 146 401 4.06e-133 401
MS.gene000081.t1 MTR_2g438640 81.340 209 38 1 360 568 399 606 4.17e-106 331
MS.gene000081.t1 MTR_5g028190 32.772 534 325 12 46 553 10 535 1.08e-79 260
MS.gene000081.t1 MTR_5g091220 30.836 574 351 11 19 550 33 602 3.18e-66 226
MS.gene000081.t1 MTR_3g055310 30.252 476 292 11 109 547 15 487 1.86e-59 206
MS.gene000081.t1 MTR_3g055310 30.323 465 284 11 120 547 24 485 4.33e-58 202
MS.gene000081.t1 MTR_3g055310 30.323 465 284 11 120 547 51 512 1.11e-57 201
MS.gene000081.t1 MTR_3g055310 31.265 419 253 9 161 547 44 459 3.68e-57 199
MS.gene000081.t1 MTR_3g055310 37.329 292 178 4 258 547 56 344 6.19e-57 195
MS.gene000081.t1 MTR_3g055310 37.329 292 178 4 258 547 119 407 1.49e-56 196
MS.gene000081.t1 MTR_5g028130 37.162 296 177 4 259 548 42 334 6.33e-56 192
MS.gene000081.t1 MTR_3g455790 25.899 556 373 9 18 538 34 585 6.22e-54 193
MS.gene000081.t1 MTR_5g028930 28.015 539 347 10 53 563 95 620 1.45e-51 187
MS.gene000081.t1 MTR_1g113760 26.465 563 348 14 15 549 20 544 7.49e-51 183
MS.gene000081.t1 MTR_1g113820 28.097 452 293 5 24 469 60 485 1.08e-50 184
MS.gene000081.t1 MTR_5g028450 36.644 292 182 2 262 550 4 295 2.33e-48 170
MS.gene000081.t1 MTR_3g452020 25.905 525 352 10 18 507 34 556 4.44e-45 168
MS.gene000081.t1 MTR_2g020510 34.211 304 183 6 259 550 390 688 8.02e-42 160
MS.gene000081.t1 MTR_5g085350 35.836 293 181 3 262 549 427 717 1.12e-41 160
MS.gene000081.t1 MTR_5g085340 36.271 295 179 4 262 549 446 738 1.26e-41 160
MS.gene000081.t1 MTR_5g085350 35.836 293 181 3 262 549 440 730 1.26e-41 160
MS.gene000081.t1 MTR_2g020450 34.219 301 186 5 259 550 393 690 8.60e-39 151
MS.gene000081.t1 MTR_2g438580 81.579 76 14 0 373 448 1 76 1.32e-38 136
MS.gene000081.t1 MTR_2g017930 34.815 270 159 4 278 537 378 640 7.89e-38 148
MS.gene000081.t1 MTR_3g058570 35.333 300 180 7 259 549 84 378 2.91e-37 142
MS.gene000081.t1 MTR_5g085360 27.577 359 215 8 232 550 377 730 1.27e-35 142
MS.gene000081.t1 MTR_3g452020 31.474 251 169 2 256 505 130 378 9.51e-33 130
MS.gene000081.t1 MTR_3g051060 30.961 281 190 3 274 550 449 729 2.17e-32 132
MS.gene000081.t1 MTR_6g017215 39.378 193 112 3 362 551 8 198 1.23e-31 122
MS.gene000081.t1 MTR_2g020470 29.934 304 176 8 259 550 392 670 1.61e-30 127
MS.gene000081.t1 MTR_3g050500 28.720 289 201 3 262 545 431 719 2.05e-29 124
MS.gene000081.t1 MTR_5g028110 35.602 191 100 4 360 548 2 171 1.86e-28 112
MS.gene000081.t1 MTR_5g028690 37.415 147 91 1 358 503 39 185 2.81e-23 99.4
MS.gene000081.t1 MTR_5g028840 31.795 195 125 4 360 548 2 194 2.79e-22 95.5
MS.gene000081.t1 MTR_1g113730 30.435 184 119 3 256 436 43 220 1.38e-20 94.0
MS.gene000081.t1 MTR_5g028900 33.758 157 91 3 362 516 4 149 2.44e-19 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000081.t1 AT5G35810 44.231 312 166 3 246 550 22 332 5.10e-83 263
MS.gene000081.t1 AT3G18670 31.492 543 337 8 26 550 59 584 1.41e-79 262
MS.gene000081.t1 AT3G18670 30.743 592 332 11 26 550 59 639 6.85e-79 261
MS.gene000081.t1 AT5G04700 31.927 545 320 13 52 550 117 656 3.08e-68 233
MS.gene000081.t1 AT5G04690 30.069 582 354 14 26 562 85 658 1.10e-67 231
MS.gene000081.t1 AT5G04690 30.576 556 351 13 26 562 85 624 4.19e-67 229
MS.gene000081.t1 AT5G04690 29.534 579 347 14 26 562 85 644 4.86e-66 227
MS.gene000081.t1 AT5G04680 30.385 520 308 13 82 550 12 528 7.77e-59 205
MS.gene000081.t1 AT5G04680 30.385 520 308 13 82 550 135 651 4.97e-58 206
MS.gene000081.t1 AT5G04730 29.380 548 330 14 67 561 57 600 5.11e-57 201
MS.gene000081.t1 AT5G04680 29.783 554 322 15 49 550 99 637 5.93e-56 199
MS.gene000081.t1 AT5G04680 36.213 301 178 4 262 550 382 680 3.45e-48 179
MS.gene000081.t1 AT3G54065 42.453 212 114 2 346 550 8 218 5.06e-47 164
MS.gene000081.t1 AT5G35830 37.430 179 102 3 6 178 80 254 8.70e-28 113

Find 87 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGCTGAAGTTGGAAATTT+TGG 0.113613 2.3:+52728149 MS.gene000081:CDS
ACCTGTTATAATTGGTAAAT+TGG 0.145911 2.3:-52726893 None:intergenic
AATCCAAATATTAGCTTTAT+AGG 0.156047 2.3:-52728927 None:intergenic
CCATTTCCACCTGTTATAAT+TGG 0.186786 2.3:-52726901 None:intergenic
GGACAACTTGAATTGGTTTC+TGG 0.195700 2.3:+52728383 MS.gene000081:CDS
CTGCCTATAAAGCTAATATT+TGG 0.224134 2.3:+52728924 MS.gene000081:CDS
TCCAATTGGTTAAATTTCTT+TGG 0.229153 2.3:+52728045 MS.gene000081:CDS
TTCAATCTCGTTCGTCAAAT+AGG 0.248483 2.3:+52728278 MS.gene000081:CDS
TTCATAACATACAACCATTA+TGG 0.263591 2.3:+52729005 MS.gene000081:CDS
AAATGTGTGATGAGTTATTA+TGG 0.290590 2.3:+52728417 MS.gene000081:CDS
TGTATCTACACTGGCATCTA+TGG 0.308183 2.3:+52727042 MS.gene000081:CDS
TCTATTCTTGTCTCGCGTTA+TGG 0.309263 2.3:+52728879 MS.gene000081:CDS
GTGCCCATCCTAGCTTGATA+TGG 0.317470 2.3:+52728192 MS.gene000081:CDS
TCCAAAGAAATTTAACCAAT+TGG 0.319665 2.3:-52728046 None:intergenic
GAAAATATTGAAATGCAAAT+AGG 0.329984 2.3:-52729041 None:intergenic
TTCTGTATTGCTGCTGCAGC+TGG 0.331708 2.3:+52726832 MS.gene000081:CDS
TCATTAACTCAATCTACTAT+TGG 0.340833 2.3:+52729117 MS.gene000081:CDS
CCAATTATAACAGGTGGAAA+TGG 0.352802 2.3:+52726901 MS.gene000081:CDS
ACCAATTTACCAATTATAAC+AGG 0.361393 2.3:+52726892 MS.gene000081:CDS
TCAATCTCGTTCGTCAAATA+GGG 0.376053 2.3:+52728279 MS.gene000081:CDS
TGCTACCATCATGCTTGTTA+TGG 0.377755 2.3:-52728967 None:intergenic
AGTATGCTCTAAACGGAAAC+TGG 0.384523 2.3:+52726629 MS.gene000081:CDS
CGAGACAGCTTTGCATCTCT+TGG 0.393587 2.3:+52727847 MS.gene000081:CDS
AACCTATGTACAAAAGTATT+GGG 0.399981 2.3:-52729070 None:intergenic
TGTAGGCCACCCATTTGTTA+TGG 0.405468 2.3:-52726701 None:intergenic
AATAGACAAGAAAATCAATA+TGG 0.415156 2.3:-52728862 None:intergenic
ACCACCGGGACAACTTGAAT+TGG 0.418197 2.3:+52728376 MS.gene000081:CDS
ACGAACGAGATTGAAGATAC+TGG 0.419677 2.3:-52728270 None:intergenic
ATATTTGATGCTGCTGAAGT+TGG 0.426249 2.3:+52728140 MS.gene000081:CDS
TCGTCAAATAGGGCATATAA+AGG 0.429101 2.3:+52728289 MS.gene000081:CDS
AAACCTATGTACAAAAGTAT+TGG 0.433272 2.3:-52729071 None:intergenic
TGCCCATCCTAGCTTGATAT+GGG 0.442976 2.3:+52728193 MS.gene000081:CDS
TGCTAACCACATTCACTTTG+TGG 0.444610 2.3:+52726744 MS.gene000081:CDS
GTTTAGAGCATACTTGTGAA+TGG 0.450188 2.3:-52726620 None:intergenic
ACATTATTGCATATTGCTGC+AGG 0.456201 2.3:+52726721 MS.gene000081:CDS
ATTTCGACAGTCATTGCAAC+CGG 0.475205 2.3:+52728711 MS.gene000081:CDS
GTGTTAGGATTTACCAGGAT+TGG 0.476840 2.3:-52727936 None:intergenic
TATATGTGATAGAGGAGTAA+AGG 0.479099 2.3:+52729171 MS.gene000081:CDS
ATTCACAAGTATGCTCTAAA+CGG 0.479559 2.3:+52726622 MS.gene000081:CDS
GGCTGCAAAATTAGCACCAC+CGG 0.480700 2.3:+52728361 MS.gene000081:CDS
TCTAAGATGCGCTTAGCTGC+TGG 0.481360 2.3:-52726652 None:intergenic
GCTGCTGCAGTTACTTTACC+AGG 0.482635 2.3:+52728741 MS.gene000081:CDS
AATCACGTCATGACCAATCC+TGG 0.491843 2.3:+52727923 MS.gene000081:CDS
GAATCCGTATCGAACCATAA+TGG 0.495930 2.3:-52729019 None:intergenic
TTTAGAGCATACTTGTGAAT+GGG 0.499082 2.3:-52726619 None:intergenic
TAAGGGAGGATGCAGAGTCT+TGG 0.502225 2.3:+52728658 MS.gene000081:CDS
TTCTTTGCTTGTATCTACAC+TGG 0.502845 2.3:+52727033 MS.gene000081:CDS
TAGGTGATGATGAAAGGAAA+AGG 0.504007 2.3:+52726578 MS.gene000081:intron
TGATGAGTTATTATGGTTTG+AGG 0.505669 2.3:+52728424 MS.gene000081:CDS
AGGAAACACTTTATTGCACT+TGG 0.508233 2.3:+52728340 MS.gene000081:CDS
TGCAAAGCAGCATAGTGAAT+GGG 0.514287 2.3:-52726931 None:intergenic
CCTGTGTCATCGGTACCGCC+TGG 0.516045 2.3:-52728759 None:intergenic
TTGCAAAGCAGCATAGTGAA+TGG 0.522983 2.3:-52726932 None:intergenic
TTAACTCAATCTACTATTGG+AGG 0.523979 2.3:+52729120 MS.gene000081:CDS
GTCATTCTCATCACGTTCGA+AGG 0.524895 2.3:-52727825 None:intergenic
AAAATGCTGCCATAACAAAT+GGG 0.534307 2.3:+52726692 MS.gene000081:CDS
ATTCACTATGCTGCTTTGCA+AGG 0.539160 2.3:+52726934 MS.gene000081:CDS
GCATCAAATATTAGTTGAGA+AGG 0.542969 2.3:-52728128 None:intergenic
ACAACCATTATGGTTCGATA+CGG 0.543537 2.3:+52729015 MS.gene000081:CDS
AAATATGATATATGTGATAG+AGG 0.556780 2.3:+52729163 MS.gene000081:CDS
GGTGGTTTATTCAAGTAGTG+TGG 0.557785 2.3:-52728786 None:intergenic
TGGAGGACTCTATCTAAGAC+TGG 0.561954 2.3:+52729137 MS.gene000081:CDS
GCAGCAATATGCAATAATGT+AGG 0.562566 2.3:-52726718 None:intergenic
AATCCCATATCAAGCTAGGA+TGG 0.565813 2.3:-52728196 None:intergenic
GTGACATATGTTGATGATGA+AGG 0.569060 2.3:+52728320 MS.gene000081:CDS
TTCAAATCCCATATCAAGCT+AGG 0.569234 2.3:-52728200 None:intergenic
CATCTCCATAACAAGCATGA+TGG 0.578014 2.3:+52728962 MS.gene000081:CDS
GGCTCAAAATCAAATGCCAA+TGG 0.578932 2.3:+52727868 MS.gene000081:CDS
AATTTACCAATTATAACAGG+TGG 0.586650 2.3:+52726895 MS.gene000081:CDS
GAAACCAATTCAAGTTGTCC+CGG 0.588953 2.3:-52728380 None:intergenic
ATCCCATATCAAGCTAGGAT+GGG 0.593606 2.3:-52728195 None:intergenic
CAATGACAAGAAGAATCCAT+TGG 0.600443 2.3:-52727884 None:intergenic
TGCACAAGAGTTATTCTCAA+AGG 0.611454 2.3:+52728623 MS.gene000081:CDS
AGACAGACACTACTCATCAA+AGG 0.611874 2.3:+52728079 MS.gene000081:CDS
ACTCCTCCACAAAGTGAATG+TGG 0.614353 2.3:-52726750 None:intergenic
ATAGAATTGCAAGATATGAA+CGG 0.623371 2.3:+52726799 MS.gene000081:CDS
GTTAATGATATCTGAAAACA+AGG 0.624168 2.3:-52729102 None:intergenic
AACTGCAGCAGCGAACACTC+CGG 0.662595 2.3:-52728730 None:intergenic
ATGCTGCCATAACAAATGGG+TGG 0.675092 2.3:+52726695 MS.gene000081:CDS
ACCAATTCAAGTTGTCCCGG+TGG 0.675110 2.3:-52728377 None:intergenic
GCTGCAAAATTAGCACCACC+GGG 0.676047 2.3:+52728362 MS.gene000081:CDS
TAACCACATTCACTTTGTGG+AGG 0.679323 2.3:+52726747 MS.gene000081:CDS
CTATGTACAAAAGTATTGGG+AGG 0.681529 2.3:-52729067 None:intergenic
CAGACACTACTCATCAAAGG+AGG 0.681755 2.3:+52728082 MS.gene000081:CDS
CCAGGCGGTACCGATGACAC+AGG 0.688773 2.3:+52728759 MS.gene000081:CDS
AAGGAAGATACAAAATGACA+TGG 0.688802 2.3:+52726953 MS.gene000081:CDS
GCTGCAGTTACTTTACCAGG+CGG 0.769401 2.3:+52728744 MS.gene000081:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGTTTGTTTAATAATT+TGG + chr2.3:52726452-52726471 MS.gene000081:intron 10.0%
!! TTAAATTTATTAAGTAAAGT+AGG - chr2.3:52728483-52728502 None:intergenic 10.0%
!! TAAAAAAAAAATTGTATTGC+AGG + chr2.3:52728002-52728021 MS.gene000081:intron 15.0%
!!! AAATAATATACTTTTCCAAT+TGG + chr2.3:52728031-52728050 MS.gene000081:CDS 15.0%
!!! ACTTTTAAATAAAAGTTTAG+AGG - chr2.3:52727665-52727684 None:intergenic 15.0%
!! AAATATGATATATGTGATAG+AGG + chr2.3:52729163-52729182 MS.gene000081:CDS 20.0%
!! AACTTATATTCATTTGGTAT+TGG + chr2.3:52727585-52727604 MS.gene000081:intron 20.0%
!! AATAGACAAGAAAATCAATA+TGG - chr2.3:52728865-52728884 None:intergenic 20.0%
!! GAAAATATTGAAATGCAAAT+AGG - chr2.3:52729044-52729063 None:intergenic 20.0%
!! GCAATTGAAAAATAATACTA+TGG - chr2.3:52727764-52727783 None:intergenic 20.0%
!! GTATAAGCTTGTAATATTAA+TGG + chr2.3:52727478-52727497 MS.gene000081:intron 20.0%
!! TAATATTACAAGCTTATACA+AGG - chr2.3:52727477-52727496 None:intergenic 20.0%
!! TAATTTAATGGTTGAATTGT+TGG - chr2.3:52727389-52727408 None:intergenic 20.0%
!!! AATCCAAATATTAGCTTTAT+AGG - chr2.3:52728930-52728949 None:intergenic 20.0%
!!! ATTGATTTTGTCATTCAAAA+AGG + chr2.3:52727221-52727240 MS.gene000081:intron 20.0%
!!! CATTTTTTAGTTTATTCTCT+TGG - chr2.3:52726678-52726697 None:intergenic 20.0%
!!! CTAAGAAAATATAAAGTGTT+AGG - chr2.3:52727954-52727973 None:intergenic 20.0%
!!! GGAAAAGTATATTATTTTTC+AGG - chr2.3:52728028-52728047 None:intergenic 20.0%
! AAATGTGTGATGAGTTATTA+TGG + chr2.3:52728417-52728436 MS.gene000081:CDS 25.0%
! AACCATCTGTTATGATATTA+AGG + chr2.3:52726503-52726522 MS.gene000081:intron 25.0%
! AACTGAAATCATGATTTGAT+TGG - chr2.3:52727627-52727646 None:intergenic 25.0%
! AATTTACCAATTATAACAGG+TGG + chr2.3:52726895-52726914 MS.gene000081:CDS 25.0%
! ACCAATTTACCAATTATAAC+AGG + chr2.3:52726892-52726911 MS.gene000081:CDS 25.0%
! ACCTGTTATAATTGGTAAAT+TGG - chr2.3:52726896-52726915 None:intergenic 25.0%
! ATACGCAACTTATATTCATT+TGG + chr2.3:52727579-52727598 MS.gene000081:intron 25.0%
! ATAGAATTGCAAGATATGAA+CGG + chr2.3:52726799-52726818 MS.gene000081:CDS 25.0%
! GTTAATGATATCTGAAAACA+AGG - chr2.3:52729105-52729124 None:intergenic 25.0%
! TACCTTAATATCATAACAGA+TGG - chr2.3:52726508-52726527 None:intergenic 25.0%
! TCATTAACTCAATCTACTAT+TGG + chr2.3:52729117-52729136 MS.gene000081:CDS 25.0%
! TCCAAAGAAATTTAACCAAT+TGG - chr2.3:52728049-52728068 None:intergenic 25.0%
! TCCAATTGGTTAAATTTCTT+TGG + chr2.3:52728045-52728064 MS.gene000081:CDS 25.0%
! TTCATAACATACAACCATTA+TGG + chr2.3:52729005-52729024 MS.gene000081:CDS 25.0%
! TTTCAGTTGTAAATATGTTG+AGG + chr2.3:52727639-52727658 MS.gene000081:intron 25.0%
!! AAACCTATGTACAAAAGTAT+TGG - chr2.3:52729074-52729093 None:intergenic 25.0%
!! AAAGGAGCATAAAAAACTAA+GGG + chr2.3:52728641-52728660 MS.gene000081:CDS 25.0%
!! TCGAAATACTCTTATGTTTT+AGG + chr2.3:52726559-52726578 MS.gene000081:intron 25.0%
!!! AACCTATGTACAAAAGTATT+GGG - chr2.3:52729073-52729092 None:intergenic 25.0%
!!! TTATTTTTCAATTGCAGACT+TGG + chr2.3:52727769-52727788 MS.gene000081:intron 25.0%
!!! TTTTCTTAGTTCTTTTACAC+TGG + chr2.3:52727965-52727984 MS.gene000081:intron 25.0%
!!! TTTTTCATCTTTATTTGTGC+TGG - chr2.3:52728588-52728607 None:intergenic 25.0%
AAAAATGCTGCCATAACAAA+TGG + chr2.3:52726691-52726710 MS.gene000081:CDS 30.0%
AAAATGCTGCCATAACAAAT+GGG + chr2.3:52726692-52726711 MS.gene000081:CDS 30.0%
AAATGTCGCTAAAACATTAG+TGG - chr2.3:52727135-52727154 None:intergenic 30.0%
AAGGAAGATACAAAATGACA+TGG + chr2.3:52726953-52726972 MS.gene000081:CDS 30.0%
ACTCAAAATTCTCTATCTAG+AGG - chr2.3:52727413-52727432 None:intergenic 30.0%
ATGACAATCCTTGTAAGTAT+CGG + chr2.3:52727440-52727459 MS.gene000081:intron 30.0%
CCTTAAAAATGGTAAGAGAA+AGG + chr2.3:52727792-52727811 MS.gene000081:CDS 30.0%
CTATCTAGAGGCTAATTTAA+TGG - chr2.3:52727401-52727420 None:intergenic 30.0%
CTGCCTATAAAGCTAATATT+TGG + chr2.3:52728924-52728943 MS.gene000081:CDS 30.0%
GCATCAAATATTAGTTGAGA+AGG - chr2.3:52728131-52728150 None:intergenic 30.0%
TAGGGACAAGGTTATTTAAT+TGG - chr2.3:52727346-52727365 None:intergenic 30.0%
TGATGAGTTATTATGGTTTG+AGG + chr2.3:52728424-52728443 MS.gene000081:CDS 30.0%
TTAACTCAATCTACTATTGG+AGG + chr2.3:52729120-52729139 MS.gene000081:CDS 30.0%
! ATAAAGTGTTAGGATTTACC+AGG - chr2.3:52727944-52727963 None:intergenic 30.0%
! ATTCACAAGTATGCTCTAAA+CGG + chr2.3:52726622-52726641 MS.gene000081:CDS 30.0%
! CAAAGGAGCATAAAAAACTA+AGG + chr2.3:52728640-52728659 MS.gene000081:CDS 30.0%
! CTTGAATAAACCACCATTTT+TGG + chr2.3:52728794-52728813 MS.gene000081:CDS 30.0%
! TTTAGAGCATACTTGTGAAT+GGG - chr2.3:52726622-52726641 None:intergenic 30.0%
! TTTTTGCAATCGATATGTTG+TGG - chr2.3:52727735-52727754 None:intergenic 30.0%
!! AATACATCGTTTTGATGACA+AGG + chr2.3:52726996-52727015 MS.gene000081:CDS 30.0%
!! ATGTTTTAGGTGATGATGAA+AGG + chr2.3:52726572-52726591 MS.gene000081:intron 30.0%
!! CCTTTCTCTTACCATTTTTA+AGG - chr2.3:52727795-52727814 None:intergenic 30.0%
ACAACCATTATGGTTCGATA+CGG + chr2.3:52729015-52729034 MS.gene000081:CDS 35.0%
ACATTATTGCATATTGCTGC+AGG + chr2.3:52726721-52726740 MS.gene000081:CDS 35.0%
AGGAAACACTTTATTGCACT+TGG + chr2.3:52728340-52728359 MS.gene000081:CDS 35.0%
AGGTAGTTCAATATTCTCCT+TGG + chr2.3:52726523-52726542 MS.gene000081:intron 35.0%
AGTGGAAATGTGCTAATGTT+TGG - chr2.3:52727117-52727136 None:intergenic 35.0%
ATATTTGATGCTGCTGAAGT+TGG + chr2.3:52728140-52728159 MS.gene000081:CDS 35.0%
ATTGCAAACACCAAAAATGG+TGG - chr2.3:52728807-52728826 None:intergenic 35.0%
CAATGACAAGAAGAATCCAT+TGG - chr2.3:52727887-52727906 None:intergenic 35.0%
CCAATTATAACAGGTGGAAA+TGG + chr2.3:52726901-52726920 MS.gene000081:CDS 35.0%
CCATTTCCACCTGTTATAAT+TGG - chr2.3:52726904-52726923 None:intergenic 35.0%
GAGATTGCAAACACCAAAAA+TGG - chr2.3:52728810-52728829 None:intergenic 35.0%
GATAATTGTGTATGCACACA+AGG - chr2.3:52728461-52728480 None:intergenic 35.0%
GCAGCAATATGCAATAATGT+AGG - chr2.3:52726721-52726740 None:intergenic 35.0%
GTAAAATCCCATATGCTCTA+TGG - chr2.3:52727713-52727732 None:intergenic 35.0%
GTCTTGGATGAAAAAAACTG+CGG + chr2.3:52728674-52728693 MS.gene000081:CDS 35.0%
TCAATCTCGTTCGTCAAATA+GGG + chr2.3:52728279-52728298 MS.gene000081:CDS 35.0%
TCGTCAAATAGGGCATATAA+AGG + chr2.3:52728289-52728308 MS.gene000081:CDS 35.0%
TGCACAAGAGTTATTCTCAA+AGG + chr2.3:52728623-52728642 MS.gene000081:CDS 35.0%
TTCAAATCCCATATCAAGCT+AGG - chr2.3:52728203-52728222 None:intergenic 35.0%
TTCAATCTCGTTCGTCAAAT+AGG + chr2.3:52728278-52728297 MS.gene000081:CDS 35.0%
TTCTTTGCTTGTATCTACAC+TGG + chr2.3:52727033-52727052 MS.gene000081:CDS 35.0%
! CTCCCAATACTTTTGTACAT+AGG + chr2.3:52729068-52729087 MS.gene000081:CDS 35.0%
! CTGCTGAAGTTGGAAATTTT+GGG + chr2.3:52728150-52728169 MS.gene000081:CDS 35.0%
! GTTTAGAGCATACTTGTGAA+TGG - chr2.3:52726623-52726642 None:intergenic 35.0%
! TAGGTGATGATGAAAGGAAA+AGG + chr2.3:52726578-52726597 MS.gene000081:intron 35.0%
!! CTATGTACAAAAGTATTGGG+AGG - chr2.3:52729070-52729089 None:intergenic 35.0%
!! GTGACATATGTTGATGATGA+AGG + chr2.3:52728320-52728339 MS.gene000081:CDS 35.0%
AAAACTCCATTCAACGACCT+AGG - chr2.3:52727194-52727213 None:intergenic 40.0%
AAATCCCATATGCTCTATGG+TGG - chr2.3:52727710-52727729 None:intergenic 40.0%
AATCCCATATCAAGCTAGGA+TGG - chr2.3:52728199-52728218 None:intergenic 40.0%
ACGAACGAGATTGAAGATAC+TGG - chr2.3:52728273-52728292 None:intergenic 40.0%
AGACAGACACTACTCATCAA+AGG + chr2.3:52728079-52728098 MS.gene000081:CDS 40.0%
ATCCCATATCAAGCTAGGAT+GGG - chr2.3:52728198-52728217 None:intergenic 40.0%
ATTTCGACAGTCATTGCAAC+CGG + chr2.3:52728711-52728730 MS.gene000081:CDS 40.0%
CATCTCCATAACAAGCATGA+TGG + chr2.3:52728962-52728981 MS.gene000081:CDS 40.0%
GAATCCGTATCGAACCATAA+TGG - chr2.3:52729022-52729041 None:intergenic 40.0%
GGAAATGTGCTAATGTTTGG+TGG - chr2.3:52727114-52727133 None:intergenic 40.0%
GGAGCATAAAAAACTAAGGG+AGG + chr2.3:52728644-52728663 MS.gene000081:CDS 40.0%
GGCTCAAAATCAAATGCCAA+TGG + chr2.3:52727868-52727887 MS.gene000081:CDS 40.0%
GGTGGTTTATTCAAGTAGTG+TGG - chr2.3:52728789-52728808 None:intergenic 40.0%
GTGTTAGGATTTACCAGGAT+TGG - chr2.3:52727939-52727958 None:intergenic 40.0%
TAACCACATTCACTTTGTGG+AGG + chr2.3:52726747-52726766 MS.gene000081:CDS 40.0%
TCTATTCTTGTCTCGCGTTA+TGG + chr2.3:52728879-52728898 MS.gene000081:CDS 40.0%
TGCAAAGCAGCATAGTGAAT+GGG - chr2.3:52726934-52726953 None:intergenic 40.0%
TGCTAACCACATTCACTTTG+TGG + chr2.3:52726744-52726763 MS.gene000081:CDS 40.0%
TGTATCTACACTGGCATCTA+TGG + chr2.3:52727042-52727061 MS.gene000081:CDS 40.0%
TTGCAAAGCAGCATAGTGAA+TGG - chr2.3:52726935-52726954 None:intergenic 40.0%
! AGAGTATTTCGATCGTGTGA+AGG - chr2.3:52726551-52726570 None:intergenic 40.0%
! AGTATGCTCTAAACGGAAAC+TGG + chr2.3:52726629-52726648 MS.gene000081:CDS 40.0%
! GCTGCTGAAGTTGGAAATTT+TGG + chr2.3:52728149-52728168 MS.gene000081:CDS 40.0%
! GGACAACTTGAATTGGTTTC+TGG + chr2.3:52728383-52728402 MS.gene000081:CDS 40.0%
! TGCTACCATCATGCTTGTTA+TGG - chr2.3:52728970-52728989 None:intergenic 40.0%
!! ATCGTTTTGATGACAAGGAC+AGG + chr2.3:52727001-52727020 MS.gene000081:CDS 40.0%
!! ATTCACTATGCTGCTTTGCA+AGG + chr2.3:52726934-52726953 MS.gene000081:CDS 40.0%
!! CGTTGAATGGAGTTTTTCAG+AGG + chr2.3:52727198-52727217 MS.gene000081:intron 40.0%
!! GAAACCAATTCAAGTTGTCC+CGG - chr2.3:52728383-52728402 None:intergenic 40.0%
!! TGCACATAGCTGTTTTGCAT+CGG + chr2.3:52728243-52728262 MS.gene000081:CDS 40.0%
!! TGCAGACTTGGCCTTAAAAA+TGG + chr2.3:52727781-52727800 MS.gene000081:intron 40.0%
AATCACGTCATGACCAATCC+TGG + chr2.3:52727923-52727942 MS.gene000081:CDS 45.0%
ACTCCTCCACAAAGTGAATG+TGG - chr2.3:52726753-52726772 None:intergenic 45.0%
ATAGCTTGATCGATCGGTGT+AGG + chr2.3:52727518-52727537 MS.gene000081:intron 45.0%
ATGCTGCCATAACAAATGGG+TGG + chr2.3:52726695-52726714 MS.gene000081:CDS 45.0%
CAGACACTACTCATCAAAGG+AGG + chr2.3:52728082-52728101 MS.gene000081:CDS 45.0%
CAGACGATAGCTTGATCGAT+CGG + chr2.3:52727512-52727531 MS.gene000081:intron 45.0%
CCTTGTCCCTAAATCGATTG+CGG + chr2.3:52727355-52727374 MS.gene000081:intron 45.0%
GTCAGATGCCGATACTTACA+AGG - chr2.3:52727451-52727470 None:intergenic 45.0%
GTCATTCTCATCACGTTCGA+AGG - chr2.3:52727828-52727847 None:intergenic 45.0%
GTTGCAACTTTAGCCGTCAT+CGG + chr2.3:52727683-52727702 MS.gene000081:intron 45.0%
TGCCCATCCTAGCTTGATAT+GGG + chr2.3:52728193-52728212 MS.gene000081:CDS 45.0%
! GTGTGGTTTTCCTGTGTCAT+CGG - chr2.3:52728772-52728791 None:intergenic 45.0%
! TCTCTGCCGCAATCGATTTA+GGG - chr2.3:52727364-52727383 None:intergenic 45.0%
! TGGAGGACTCTATCTAAGAC+TGG + chr2.3:52729137-52729156 MS.gene000081:CDS 45.0%
! TGTAGGCCACCCATTTGTTA+TGG - chr2.3:52726704-52726723 None:intergenic 45.0%
ACCACCGGGACAACTTGAAT+TGG + chr2.3:52728376-52728395 MS.gene000081:CDS 50.0%
CGAGACAGCTTTGCATCTCT+TGG + chr2.3:52727847-52727866 MS.gene000081:CDS 50.0%
CGGTCCACCATAGAGCATAT+GGG + chr2.3:52727703-52727722 MS.gene000081:intron 50.0%
GCTGCAAAATTAGCACCACC+GGG + chr2.3:52728362-52728381 MS.gene000081:CDS 50.0%
GGCTGCAAAATTAGCACCAC+CGG + chr2.3:52728361-52728380 MS.gene000081:CDS 50.0%
GTGCCCATCCTAGCTTGATA+TGG + chr2.3:52728192-52728211 MS.gene000081:CDS 50.0%
GTGTCTCCTAGGTCGTTGAA+TGG + chr2.3:52727185-52727204 MS.gene000081:intron 50.0%
TAAGGGAGGATGCAGAGTCT+TGG + chr2.3:52728658-52728677 MS.gene000081:CDS 50.0%
TCGGTCCACCATAGAGCATA+TGG + chr2.3:52727702-52727721 MS.gene000081:intron 50.0%
TTCTGTATTGCTGCTGCAGC+TGG + chr2.3:52726832-52726851 MS.gene000081:CDS 50.0%
! CCGCAATCGATTTAGGGACA+AGG - chr2.3:52727358-52727377 None:intergenic 50.0%
! GCTGCAGTTACTTTACCAGG+CGG + chr2.3:52728744-52728763 MS.gene000081:CDS 50.0%
! GCTGCTGCAGTTACTTTACC+AGG + chr2.3:52728741-52728760 MS.gene000081:CDS 50.0%
! GTCTCTGCCGCAATCGATTT+AGG - chr2.3:52727365-52727384 None:intergenic 50.0%
! TCTAAGATGCGCTTAGCTGC+TGG - chr2.3:52726655-52726674 None:intergenic 50.0%
!! ACCAATTCAAGTTGTCCCGG+TGG - chr2.3:52728380-52728399 None:intergenic 50.0%
!! CGACTCAAAGAGTGTCTCCT+AGG + chr2.3:52727174-52727193 MS.gene000081:intron 50.0%
!! TCGATCGTGTGAAGGTACCA+AGG - chr2.3:52726543-52726562 None:intergenic 50.0%
AACTGCAGCAGCGAACACTC+CGG - chr2.3:52728733-52728752 None:intergenic 55.0%
!! GCTCTATGGTGGACCGATGA+CGG - chr2.3:52727699-52727718 None:intergenic 55.0%
CCTGTGTCATCGGTACCGCC+TGG - chr2.3:52728762-52728781 None:intergenic 65.0%
!! CCAGGCGGTACCGATGACAC+AGG + chr2.3:52728759-52728778 MS.gene000081:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 52726441 52729190 52726441 ID=MS.gene000081
chr2.3 mRNA 52726441 52729190 52726441 ID=MS.gene000081.t1;Parent=MS.gene000081
chr2.3 exon 52726441 52726456 52726441 ID=MS.gene000081.t1.exon1;Parent=MS.gene000081.t1
chr2.3 CDS 52726441 52726456 52726441 ID=cds.MS.gene000081.t1;Parent=MS.gene000081.t1
chr2.3 exon 52726581 52727063 52726581 ID=MS.gene000081.t1.exon2;Parent=MS.gene000081.t1
chr2.3 CDS 52726581 52727063 52726581 ID=cds.MS.gene000081.t1;Parent=MS.gene000081.t1
chr2.3 exon 52727786 52727944 52727786 ID=MS.gene000081.t1.exon3;Parent=MS.gene000081.t1
chr2.3 CDS 52727786 52727944 52727786 ID=cds.MS.gene000081.t1;Parent=MS.gene000081.t1
chr2.3 exon 52728024 52728445 52728024 ID=MS.gene000081.t1.exon4;Parent=MS.gene000081.t1
chr2.3 CDS 52728024 52728445 52728024 ID=cds.MS.gene000081.t1;Parent=MS.gene000081.t1
chr2.3 exon 52728564 52729190 52728564 ID=MS.gene000081.t1.exon5;Parent=MS.gene000081.t1
chr2.3 CDS 52728564 52729190 52728564 ID=cds.MS.gene000081.t1;Parent=MS.gene000081.t1
Gene Sequence

>MS.gene000081

ATGAGTCAGGAAATATGTTTGTTTAATAATTTGGAATTGTTTTTTTCTTAAAATATTTCTTGAACCATCTGTTATGATATTAAGGTAGTTCAATATTCTCCTTGGTACCTTCACACGATCGAAATACTCTTATGTTTTAGGTGATGATGAAAGGAAAAGGTATCTCAAGCTTTGTGTTCCCATTCACAAGTATGCTCTAAACGGAAACTGGCCAGCAGCTAAGCGCATCTTAGACCAAGAGAATAAACTAAAAAATGCTGCCATAACAAATGGGTGGCCTACATTATTGCATATTGCTGCAGGTGCTAACCACATTCACTTTGTGGAGGAGTTATTGAAATTGCTTGATGACAAAGATATAGAATTGCAAGATATGAACGGAAACACTGCTTTCTGTATTGCTGCTGCAGCTGGAAACATTGAAATTGTTGACTTAATGCTGAAAATAAACACCAATTTACCAATTATAACAGGTGGAAATGGAAAAGCTCCCATTCACTATGCTGCTTTGCAAGGAAGATACAAAATGACATGGCATCTATATAATCAAAGTACAATACATCGTTTTGATGACAAGGACAGGAATTTATTGTTCTTTGCTTGTATCTACACTGGCATCTATGGTAAGCATTATTAACAGTTGTAATTCATTATTAACATTAGCACATTTCCACCAAACATTAGCACATTTCCACTAATGTTTTAGCGACATTTTACAAATCAATCTTTAAATCGACTCAAAGAGTGTCTCCTAGGTCGTTGAATGGAGTTTTTCAGAGGATTGATTTTGTCATTCAAAAAGGACTAACGACGCAATTCATTGCTCACTTGCATTTCATTCATGTGTAATTTCTTTCATCTTAAATATTGAACATTGCAATAATTAGAGACTAACTTAGTGACCAATTAAATAACCTTGTCCCTAAATCGATTGCGGCAGAGACTCCAACAATTCAACCATTAAATTAGCCTCTAGATAGAGAATTTTGAGTAGTTCATATGACAATCCTTGTAAGTATCGGCATCTGACAATCCTTGTATAAGCTTGTAATATTAATGGTTTTCACATATCAGACGATAGCTTGATCGATCGGTGTAGGTTTGACGATAAACAAGTCAAACAAATATTAATGCTTTAATACGCAACTTATATTCATTTGGTATTGGTTTTGTTTAATCAACACCAATCAAATCATGATTTCAGTTGTAAATATGTTGAGGCCTCTAAACTTTTATTTAAAAGTTGCAACTTTAGCCGTCATCGGTCCACCATAGAGCATATGGGATTTTACCACAACATATCGATTGCAAAAATACTGACCATAGTATTATTTTTCAATTGCAGACTTGGCCTTAAAAATGGTAAGAGAAAGGAATGAACTAGCCTTCGAACGTGATGAGAATGACGAGACAGCTTTGCATCTCTTGGCTCAAAATCAAATGCCAATGGATTCTTCTTGTCATTGTCTAGAATATGATCATAATCACGTCATGACCAATCCTGGTAAATCCTAACACTTTATATTTTCTTAGTTCTTTTACACTGGTTTCATTAACTTAGTAAAAAAAAAATTGTATTGCAGGCCTGAAAAATAATATACTTTTCCAATTGGTTAAATTTCTTTGGACAACGATTCTAGACAGACACTACTCATCAAAGGAGGAGTTAAATAAAATCATTAATCTACCTTCTCAACTAATATTTGATGCTGCTGAAGTTGGAAATTTTGGGTTCTTATCAGAACTTATTAGTGCCCATCCTAGCTTGATATGGGATTTGAATAGCAAAAATCACACGATATTGCACATAGCTGTTTTGCATCGGCATGCCAGTATCTTCAATCTCGTTCGTCAAATAGGGCATATAAAGGATATCATAGTGACATATGTTGATGATGAAGGAAACACTTTATTGCACTTGGCTGCAAAATTAGCACCACCGGGACAACTTGAATTGGTTTCTGGAGCAGCTTTTCAAATGTGTGATGAGTTATTATGGTTTGAGGTATAATGCAAACCTTGTGTGCATACACAATTATCCTACTTTACTTAATAAATTTAACTCTAAATTTATTTTTACATTTCTATATTTTATCTAACATATATACATTTTCATATAATAGAATGTAAAGAAGATAATGTTGCCAGCACAAATAAAGATGAAAAACTCTGAAGACTTGACTGCACAAGAGTTATTCTCAAAGGAGCATAAAAAACTAAGGGAGGATGCAGAGTCTTGGATGAAAAAAACTGCGGAGTCGTGTATGCTAATTTCGACAGTCATTGCAACCGGAGTGTTCGCTGCTGCAGTTACTTTACCAGGCGGTACCGATGACACAGGAAAACCACACTACTTGAATAAACCACCATTTTTGGTGTTTGCAATCTCAGATGCATCTGCATTCATTTCATCTTCGACTGCCATATTGATTTTCTTGTCTATTCTTGTCTCGCGTTATGGAGAGTATGACTTCTATAAATCACTGCCTATAAAGCTAATATTTGGATTGATCACATTATTCATCTCCATAACAAGCATGATGGTAGCACTTAGTAGTTGCTTCTTCATAACATACAACCATTATGGTTCGATACGGATTCCTATTTGCATTTCAATATTTTCTTTCCTCCCAATACTTTTGTACATAGGTTTGCATTTGTCCTTGTTTTCAGATATCATTAACTCAATCTACTATTGGAGGACTCTATCTAAGACTGGTGAAAATATGATATATGTGATAGAGGAGTAA
Protein sequence

>MS.gene000081.t1

MSQEICDDERKRYLKLCVPIHKYALNGNWPAAKRILDQENKLKNAAITNGWPTLLHIAAGANHIHFVEELLKLLDDKDIELQDMNGNTAFCIAAAAGNIEIVDLMLKINTNLPIITGGNGKAPIHYAALQGRYKMTWHLYNQSTIHRFDDKDRNLLFFACIYTGIYDLALKMVRERNELAFERDENDETALHLLAQNQMPMDSSCHCLEYDHNHVMTNPGLKNNILFQLVKFLWTTILDRHYSSKEELNKIINLPSQLIFDAAEVGNFGFLSELISAHPSLIWDLNSKNHTILHIAVLHRHASIFNLVRQIGHIKDIIVTYVDDEGNTLLHLAAKLAPPGQLELVSGAAFQMCDELLWFENVKKIMLPAQIKMKNSEDLTAQELFSKEHKKLREDAESWMKKTAESCMLISTVIATGVFAAAVTLPGGTDDTGKPHYLNKPPFLVFAISDASAFISSSTAILIFLSILVSRYGEYDFYKSLPIKLIFGLITLFISITSMMVALSSCFFITYNHYGSIRIPICISIFSFLPILLYIGLHLSLFSDIINSIYYWRTLSKTGENMIYVIEE