AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85145

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85145.t1 MTR_2g101320 91.549 639 14 1 1 599 1 639 0.0 1208
MS.gene85145.t1 MTR_2g101310 80.751 639 82 2 1 599 1 638 0.0 1016
MS.gene85145.t1 MTR_2g101310 85.089 503 74 1 1 503 1 502 0.0 869
MS.gene85145.t1 MTR_2g096190 22.813 583 321 15 71 592 28 542 3.12e-33 134
MS.gene85145.t1 MTR_2g096190 26.293 232 168 2 31 260 61 291 1.35e-20 95.9
MS.gene85145.t1 MTR_4g062100 22.801 557 296 14 71 567 32 514 6.87e-33 133
MS.gene85145.t1 MTR_4g062100 23.636 275 187 5 23 278 18 288 5.01e-19 91.3
MS.gene85145.t1 MTR_7g117620 23.252 572 318 14 71 592 44 544 1.08e-32 132
MS.gene85145.t1 MTR_7g117620 30.638 235 153 4 38 267 83 312 1.35e-18 89.7
MS.gene85145.t1 MTR_7g100390 21.925 561 298 14 71 569 11 493 1.64e-26 114
MS.gene85145.t1 MTR_7g100390 25.475 263 191 2 2 259 11 273 2.00e-21 98.6
MS.gene85145.t1 MTR_1g032100 22.763 514 338 13 101 592 53 529 1.65e-25 110
MS.gene85145.t1 MTR_1g115575 27.922 308 205 5 37 341 74 367 4.15e-22 100
MS.gene85145.t1 MTR_1g115575 25.703 249 182 3 1 247 96 343 6.72e-21 97.1
MS.gene85145.t1 MTR_8g105260 22.479 476 311 13 138 592 98 536 1.82e-20 95.5
MS.gene85145.t1 MTR_8g105260 25.346 217 159 3 33 247 60 275 5.98e-13 72.0
MS.gene85145.t1 MTR_7g099570 29.437 231 151 5 137 358 40 267 3.61e-19 91.7
MS.gene85145.t1 MTR_8g072510 29.630 216 132 5 137 341 41 247 9.18e-19 89.7
MS.gene85145.t1 MTR_3g105650 25.833 240 165 3 111 338 8 246 9.93e-19 89.4
MS.gene85145.t1 MTR_8g069880 27.888 251 152 5 131 370 32 264 1.23e-18 89.4
MS.gene85145.t1 MTR_5g085480 27.612 268 181 7 100 358 46 309 3.06e-18 88.6
MS.gene85145.t1 MTR_8g039620 27.948 229 138 5 145 363 53 264 3.72e-18 87.8
MS.gene85145.t1 MTR_7g021090 25.185 270 173 6 111 368 12 264 5.28e-18 87.0
MS.gene85145.t1 MTR_5g085480 27.612 268 181 7 100 358 206 469 5.60e-18 88.2
MS.gene85145.t1 MTR_8g073260 27.734 256 165 4 110 353 13 260 1.09e-17 86.3
MS.gene85145.t1 MTR_8g062410 27.854 219 141 3 134 342 38 249 5.44e-17 84.3
MS.gene85145.t1 MTR_8g066670 26.515 264 163 6 96 341 1063 1313 7.11e-16 82.0
MS.gene85145.t1 MTR_7g100430 27.111 225 162 2 24 247 112 335 7.43e-16 81.3
MS.gene85145.t1 MTR_7g100430 29.064 203 139 2 50 249 103 303 9.66e-16 80.9
MS.gene85145.t1 MTR_1g069110 20.833 360 190 5 134 491 46 312 8.61e-15 77.4
MS.gene85145.t1 MTR_3g085830 27.820 266 153 8 145 396 223 463 1.31e-14 77.4
MS.gene85145.t1 MTR_8g467780 27.638 199 125 3 137 316 42 240 1.64e-14 76.6
MS.gene85145.t1 MTR_3g016210 25.833 240 142 5 144 363 48 271 2.16e-14 76.3
MS.gene85145.t1 MTR_1g059930 28.436 211 127 5 145 342 51 250 4.94e-14 75.1
MS.gene85145.t1 MTR_8g080080 24.800 375 212 14 40 375 106 449 5.00e-14 75.5
MS.gene85145.t1 MTR_8g040930 24.670 227 143 4 149 363 2 212 6.23e-14 74.3
MS.gene85145.t1 MTR_8g041240 24.670 227 143 4 149 363 2 212 6.23e-14 74.3
MS.gene85145.t1 MTR_7g451420 25.267 281 176 9 110 376 11 271 9.48e-14 73.2
MS.gene85145.t1 MTR_2g033050 25.664 226 156 6 100 317 161 382 2.55e-13 72.4
MS.gene85145.t1 MTR_5g085500 20.843 427 269 9 168 556 1 396 4.78e-12 68.9
MS.gene85145.t1 MTR_6g027840 24.176 273 178 8 63 314 170 434 2.23e-11 67.0
MS.gene85145.t1 MTR_8g068990 26.147 218 129 6 137 342 43 240 3.05e-11 66.2
MS.gene85145.t1 MTR_5g043640 29.121 182 121 6 101 280 51 226 6.91e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85145.t1 AT1G10340 32.562 648 315 15 3 599 2 578 1.33e-91 294
MS.gene85145.t1 AT1G10340 32.099 648 314 16 3 599 2 574 2.29e-88 285
MS.gene85145.t1 AT2G24600 31.420 662 323 17 5 599 4 601 3.27e-77 256
MS.gene85145.t1 AT2G24600 31.420 662 323 17 5 599 4 601 3.27e-77 256
MS.gene85145.t1 AT2G24600 31.994 622 319 17 5 599 4 548 3.53e-74 247
MS.gene85145.t1 AT2G24600 31.994 622 319 17 5 599 4 548 3.53e-74 247
MS.gene85145.t1 AT5G54710 28.114 594 315 11 33 583 65 589 4.28e-70 238
MS.gene85145.t1 AT5G54710 28.114 594 315 11 33 583 110 634 2.42e-69 236
MS.gene85145.t1 AT5G50140 28.080 552 307 10 115 599 7 535 2.53e-55 196
MS.gene85145.t1 AT1G34050 33.125 320 206 3 1 318 17 330 4.95e-49 179
MS.gene85145.t1 AT1G34050 39.759 166 60 1 437 562 375 540 1.42e-28 120
MS.gene85145.t1 AT2G31820 23.680 587 290 17 37 571 152 632 1.29e-34 139
MS.gene85145.t1 AT1G05640 23.718 624 313 20 3 571 82 597 9.71e-33 134
MS.gene85145.t1 AT5G54700 27.882 373 200 8 192 562 17 322 1.83e-31 125
MS.gene85145.t1 AT1G07710 23.022 556 305 15 71 572 28 514 7.90e-30 124
MS.gene85145.t1 AT1G07710 28.862 246 167 5 20 259 47 290 1.45e-20 95.9
MS.gene85145.t1 AT1G07710 24.910 277 198 5 13 283 6 278 2.73e-17 85.9
MS.gene85145.t1 AT1G07710 23.022 556 305 15 71 572 28 514 7.90e-30 124
MS.gene85145.t1 AT1G07710 28.862 246 167 5 20 259 47 290 1.45e-20 95.9
MS.gene85145.t1 AT1G07710 24.910 277 198 5 13 283 6 278 2.73e-17 85.9
MS.gene85145.t1 AT5G02620 27.083 432 231 11 71 488 18 379 8.02e-30 124
MS.gene85145.t1 AT5G02620 24.060 266 197 2 2 262 18 283 7.68e-20 93.6
MS.gene85145.t1 AT5G02620 24.468 282 186 7 28 295 12 280 2.54e-15 79.3
MS.gene85145.t1 AT5G02620 27.083 432 231 11 71 488 18 379 8.02e-30 124
MS.gene85145.t1 AT5G02620 24.060 266 197 2 2 262 18 283 7.68e-20 93.6
MS.gene85145.t1 AT5G02620 24.468 282 186 7 28 295 12 280 2.54e-15 79.3
MS.gene85145.t1 AT5G02620 27.083 432 231 11 71 488 18 379 8.02e-30 124
MS.gene85145.t1 AT5G02620 24.060 266 197 2 2 262 18 283 7.68e-20 93.6
MS.gene85145.t1 AT5G02620 24.468 282 186 7 28 295 12 280 2.54e-15 79.3
MS.gene85145.t1 AT2G01680 22.151 465 289 9 101 556 56 456 5.82e-27 115
MS.gene85145.t1 AT3G12360 22.705 599 328 16 37 591 75 582 1.48e-25 111
MS.gene85145.t1 AT3G12360 25.461 271 173 5 2 247 75 341 1.43e-18 90.1
MS.gene85145.t1 AT3G12360 22.705 599 328 16 37 591 63 570 2.01e-25 111
MS.gene85145.t1 AT3G12360 27.426 237 161 5 17 247 98 329 1.76e-18 89.7
MS.gene85145.t1 AT5G02620 24.779 452 239 13 71 488 18 402 1.17e-23 105
MS.gene85145.t1 AT5G02620 23.906 297 192 7 28 295 12 303 5.22e-15 78.6
MS.gene85145.t1 AT5G02620 24.779 452 239 13 71 488 18 402 1.17e-23 105
MS.gene85145.t1 AT5G02620 23.906 297 192 7 28 295 12 303 5.22e-15 78.6
MS.gene85145.t1 AT3G09550 25.649 308 209 5 37 341 89 379 4.46e-22 100
MS.gene85145.t1 AT3G09550 25.649 308 209 5 37 341 89 379 4.46e-22 100
MS.gene85145.t1 AT3G09550 25.649 308 209 5 37 341 96 386 4.93e-22 100
MS.gene85145.t1 AT1G03670 25.234 321 221 4 14 323 92 404 5.18e-22 100
MS.gene85145.t1 AT4G03500 24.501 351 244 6 8 341 109 455 5.83e-22 100
MS.gene85145.t1 AT4G03500 24.501 351 244 6 8 341 86 432 6.76e-22 100
MS.gene85145.t1 AT4G03500 24.501 351 244 6 8 341 86 432 6.76e-22 100
MS.gene85145.t1 AT5G54700 25.189 397 191 11 156 532 19 329 2.90e-21 97.8
MS.gene85145.t1 AT5G60070 30.396 227 148 5 99 319 68 290 6.02e-21 97.1
MS.gene85145.t1 AT5G60070 23.179 302 190 7 2 295 33 300 1.87e-16 83.2
MS.gene85145.t1 AT5G54610 32.020 203 129 2 145 339 44 245 1.03e-19 92.4
MS.gene85145.t1 AT4G10720 30.097 206 134 2 144 339 43 248 1.70e-18 88.6
MS.gene85145.t1 AT4G10720 30.097 206 134 2 144 339 43 248 2.23e-18 88.6
MS.gene85145.t1 AT5G54620 30.806 211 135 2 145 345 44 253 2.84e-18 88.2
MS.gene85145.t1 AT4G14390 25.217 345 195 6 34 319 111 451 3.32e-18 88.6
MS.gene85145.t1 AT4G03450 25.197 381 223 9 1 323 36 412 5.26e-18 88.2
MS.gene85145.t1 AT4G14390 23.150 419 256 7 1 355 98 514 5.81e-18 88.2
MS.gene85145.t1 AT4G03450 23.846 390 217 8 1 323 36 412 8.48e-18 87.8
MS.gene85145.t1 AT4G14390 23.934 422 250 9 1 356 79 495 1.16e-17 87.4
MS.gene85145.t1 AT4G14390 23.990 421 249 9 1 355 98 513 1.61e-17 87.0
MS.gene85145.t1 AT5G51160 28.729 181 121 2 168 340 43 223 2.19e-17 85.5
MS.gene85145.t1 AT4G03460 24.058 345 234 7 20 340 67 407 2.38e-17 86.3
MS.gene85145.t1 AT4G03460 24.058 345 234 7 20 340 99 439 3.11e-17 85.9
MS.gene85145.t1 AT4G03490 26.552 290 200 4 61 343 135 418 1.05e-16 84.3
MS.gene85145.t1 AT4G03490 26.552 290 200 4 61 343 135 418 1.18e-16 84.3
MS.gene85145.t1 AT4G03490 26.552 290 200 4 61 343 125 408 1.39e-16 83.6
MS.gene85145.t1 AT4G14400 21.600 375 234 5 1 316 67 440 1.34e-15 80.9
MS.gene85145.t1 AT4G14400 21.600 375 234 5 1 316 1 374 1.85e-15 80.1
MS.gene85145.t1 AT4G14400 21.600 375 234 5 1 316 1 374 1.85e-15 80.1
MS.gene85145.t1 AT4G03440 24.566 346 196 11 39 323 155 496 1.44e-14 77.4
MS.gene85145.t1 AT4G03440 24.566 346 196 11 39 323 155 496 1.44e-14 77.4
MS.gene85145.t1 AT4G03440 24.779 339 190 11 39 316 162 496 1.58e-14 77.4
MS.gene85145.t1 AT5G15500 28.037 214 134 4 144 342 44 252 1.61e-14 76.6
MS.gene85145.t1 AT1G14500 25.333 225 157 3 144 357 43 267 2.38e-14 75.9
MS.gene85145.t1 AT4G05040 25.606 289 188 4 33 321 194 455 4.87e-14 75.5
MS.gene85145.t1 AT4G05040 25.606 289 188 4 33 321 194 455 4.87e-14 75.5
MS.gene85145.t1 AT4G05040 25.606 289 188 4 33 321 194 455 4.87e-14 75.5
MS.gene85145.t1 AT4G05040 25.606 289 188 4 33 321 194 455 4.87e-14 75.5
MS.gene85145.t1 AT4G05040 25.606 289 188 4 33 321 194 455 4.87e-14 75.5
MS.gene85145.t1 AT4G05040 25.606 289 188 4 33 321 194 455 4.87e-14 75.5
MS.gene85145.t1 AT4G05040 25.606 289 188 4 33 321 194 455 4.87e-14 75.5
MS.gene85145.t1 AT4G03470 24.484 339 203 6 33 321 116 451 4.98e-14 75.9
MS.gene85145.t1 AT3G04140 26.911 327 190 10 40 339 106 410 1.69e-13 73.9
MS.gene85145.t1 AT4G03480 24.000 250 156 6 95 316 293 536 1.52e-12 70.9
MS.gene85145.t1 AT4G03480 24.000 250 156 6 95 316 296 539 1.59e-12 70.9
MS.gene85145.t1 AT3G01750 24.786 234 155 5 169 381 222 455 7.04e-12 68.9
MS.gene85145.t1 AT3G01750 24.786 234 155 5 169 381 222 455 7.04e-12 68.9

Find 119 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTACATCTTGCAGTTTC+AGG 0.113729 2.4:+3427213 MS.gene85145:CDS
ATTCTCCCTCGCCAGCTTAT+AGG 0.229102 2.4:+3427781 MS.gene85145:CDS
GTTAGCACTTCCATTTCATT+AGG 0.254868 2.4:-3427828 None:intergenic
ACCACCAGCTCTGATAAATA+TGG 0.274894 2.4:-3427709 None:intergenic
CGAGAAGTAGTATGGATTAT+AGG 0.280582 2.4:-3427952 None:intergenic
CAAAACAGCCACTAATACTA+TGG 0.309601 2.4:-3428096 None:intergenic
TTGCATCATGCTTGTAATAA+AGG 0.317495 2.4:+3426904 MS.gene85145:CDS
CATTTCAATGCAACCATACT+TGG 0.318111 2.4:-3425949 None:intergenic
TTACACCTAGCTTCCAAGTA+TGG 0.320986 2.4:+3425936 MS.gene85145:CDS
TTTCATTTAGCTGTGAGATA+TGG 0.354081 2.4:+3427108 MS.gene85145:CDS
CTATGGACAGTGTAGAAAAT+AGG 0.364824 2.4:+3427676 MS.gene85145:CDS
ATCTATCTTGGCAGTGCAAT+AGG 0.372590 2.4:-3427179 None:intergenic
TTTGTTGGCGAGAAGTAGTA+TGG 0.384661 2.4:-3427960 None:intergenic
CAAGTATGGTTGCATTGAAA+TGG 0.386813 2.4:+3425950 MS.gene85145:CDS
GTGGGTAGCTGTGGCCTTCA+TGG 0.387819 2.4:+3428349 MS.gene85145:CDS
AAGATATATCCCTAATGAAA+TGG 0.391324 2.4:+3427818 MS.gene85145:CDS
TGTCTGCAAGCCTCATGAAT+TGG 0.393222 2.4:-3426035 None:intergenic
GCACTTGGTGGTGGCTCTCT+AGG 0.397848 2.4:+3428449 MS.gene85145:CDS
CATTGGTCTTAGTGTGATGC+TGG 0.402408 2.4:+3428481 MS.gene85145:CDS
TGATCAAACCCTGCCACTCC+TGG 0.413886 2.4:-3426212 None:intergenic
TTAGCACTTCCATTTCATTA+GGG 0.416830 2.4:-3427827 None:intergenic
ATATGGATGCTATGATGCTT+TGG 0.417554 2.4:+3427125 MS.gene85145:CDS
CTGGCTATGTTGCAGCTACA+TGG 0.422155 2.4:+3428375 MS.gene85145:CDS
AGAAAGAAAAGAAAGGAAAG+TGG 0.427177 2.4:+3428533 MS.gene85145:CDS
CACTGTTGCCATATCTATCT+TGG 0.430638 2.4:-3427191 None:intergenic
TGAAGGTATCATCTGTTCTC+TGG 0.432311 2.4:-3425905 None:intergenic
ATTGTGGAGCCAGGAGTGGC+AGG 0.435017 2.4:+3426203 MS.gene85145:CDS
AAGAAACACCATAGTATTAG+TGG 0.436084 2.4:+3428088 MS.gene85145:CDS
GTGCAATAGGTTTGTACCAT+TGG 0.436425 2.4:-3427166 None:intergenic
TTTCACTATGCTTGAAAATG+TGG 0.442046 2.4:-3425862 None:intergenic
CTCAAGTGATCGACGAGAAT+GGG 0.443271 2.4:+3426872 MS.gene85145:CDS
ACCATCGATTTGCCTCTCAT+TGG 0.447371 2.4:-3428179 None:intergenic
GCTGTGGCCTTCATGGCAAC+TGG 0.452630 2.4:+3428356 MS.gene85145:CDS
TTGTTAGAGAGTTGTTGAAC+AGG 0.454168 2.4:+3426836 MS.gene85145:CDS
GAAAGAAAAGAAAGGAAAGT+GGG 0.458391 2.4:+3428534 MS.gene85145:CDS
TTCTCAGCAGAAACCATGTC+AGG 0.460824 2.4:-3425996 None:intergenic
CAAAGCTGGATATTAATACT+AGG 0.465713 2.4:+3427613 MS.gene85145:CDS
CACTGCATCCCTTGATTATG+TGG 0.466763 2.4:-3428407 None:intergenic
AGCACTTGACATTCTTGATC+AGG 0.466904 2.4:+3427653 MS.gene85145:CDS
TCTCTAGGCACAATCTTCAT+TGG 0.472565 2.4:+3428464 MS.gene85145:CDS
TTGCACTGCCAAGATAGATA+TGG 0.479262 2.4:+3427183 MS.gene85145:CDS
TCGATCACTTGAGAAAGATC+TGG 0.479293 2.4:-3426862 None:intergenic
AGGCTGAGGCGATCTAGATT+CGG 0.484359 2.4:-3427901 None:intergenic
TCTCAAGTGATCGACGAGAA+TGG 0.489174 2.4:+3426871 MS.gene85145:CDS
TACTTGGAAGCTAGGTGTAA+TGG 0.492370 2.4:-3425933 None:intergenic
TTCTCCACTTGGATTTCCTA+AGG 0.493028 2.4:-3428514 None:intergenic
AAGGATACAGAGAGACTGTA+TGG 0.493841 2.4:+3426923 MS.gene85145:CDS
TCATTGCTCACAAGGTTATG+TGG 0.497097 2.4:+3428330 MS.gene85145:intron
TAGGTGTAATGGAGTGTTGA+AGG 0.497845 2.4:-3425922 None:intergenic
AGTTTCAGGTGGTCGCCATA+AGG 0.499160 2.4:+3427227 MS.gene85145:CDS
AATGGAAGTGCTAACTGAAA+TGG 0.500539 2.4:+3427836 MS.gene85145:CDS
TGCTGGTTGAACACTTCCTT+AGG 0.501298 2.4:+3428498 MS.gene85145:CDS
CTCTCACTTACTTGAGGCTG+AGG 0.501930 2.4:-3427915 None:intergenic
TTGCTGCTAGCACTTGGTGG+TGG 0.503001 2.4:+3428440 MS.gene85145:CDS
AAAATGTGAATCATACTAAG+AGG 0.503547 2.4:+3428024 MS.gene85145:CDS
CAAGGTTATGTGGGTAGCTG+TGG 0.503676 2.4:+3428340 MS.gene85145:intron
AACATAGCCAGTTGCCATGA+AGG 0.504519 2.4:-3428363 None:intergenic
TAGCCATGGCCATCTTGACT+TGG 0.505272 2.4:+3426154 MS.gene85145:CDS
TTCTTGGTGCAAGTTTCCAA+TGG 0.512450 2.4:+3427150 MS.gene85145:CDS
TTGCACCTTGCAGTAATGAA+TGG 0.521063 2.4:+3427006 MS.gene85145:CDS
CTTGATCAACAAGACAAAGC+TGG 0.522519 2.4:+3427599 MS.gene85145:CDS
TGCAACCATACTTGGAAGCT+AGG 0.529183 2.4:-3425941 None:intergenic
GCTGCTGGTATTAGTCCACC+TGG 0.532899 2.4:+3428137 MS.gene85145:CDS
TTCATTACTGCAAGGTGCAA+TGG 0.536429 2.4:-3427003 None:intergenic
TGACATTCTTGATCAGGCTA+TGG 0.536502 2.4:+3427659 MS.gene85145:CDS
ATTAAGCTTGCAAGCAGCTG+TGG 0.538390 2.4:-3426099 None:intergenic
CTATCTGAGATTGTGGAGCC+AGG 0.539701 2.4:+3426194 MS.gene85145:CDS
GTTGAATCTATCTGAGATTG+TGG 0.541115 2.4:+3426187 MS.gene85145:CDS
AAAAGAAAGGAAAGTGGGGA+TGG 0.543080 2.4:+3428539 MS.gene85145:CDS
TTTCTTATTGCTGCATCAAG+AGG 0.547893 2.4:+3426242 MS.gene85145:CDS
CAAGCCATATTTATCAGAGC+TGG 0.548722 2.4:+3427705 MS.gene85145:CDS
CAAGTGAAAACGAGGAAGAT+CGG 0.551336 2.4:-3427743 None:intergenic
TTGTGGAGCCAGGAGTGGCA+GGG 0.557101 2.4:+3426204 MS.gene85145:CDS
GTAAGTGAGAGATTTGAGAA+TGG 0.558260 2.4:+3427927 MS.gene85145:CDS
TTAGAGAGTTGTTGAACAGG+TGG 0.576847 2.4:+3426839 MS.gene85145:CDS
TGGAGCTGCTGAATCAGATA+AGG 0.577634 2.4:+3428559 MS.gene85145:CDS
TCTCAGATAGATTCAACAAG+AGG 0.578404 2.4:-3426181 None:intergenic
CATTGCTCACAAGGTTATGT+GGG 0.580255 2.4:+3428331 MS.gene85145:intron
ATTAATACTAGGAATAGTGA+AGG 0.580926 2.4:+3427624 MS.gene85145:CDS
CCTTCTTGATACACACCACC+AGG 0.581413 2.4:-3428155 None:intergenic
TGTTAGATTATGCCCTGACA+TGG 0.583786 2.4:+3425983 MS.gene85145:CDS
GATGGAGAAAGCCTATAAGC+TGG 0.584139 2.4:-3427792 None:intergenic
AAAGAAAAGAAAGGAAAGTG+GGG 0.586702 2.4:+3428535 MS.gene85145:CDS
AGAGGTTTACCAAGTCAAGA+TGG 0.587380 2.4:-3426163 None:intergenic
TTAAGCTTGCAAGCAGCTGT+GGG 0.588027 2.4:-3426098 None:intergenic
AATATGAATGATAACCCTGT+AGG 0.588775 2.4:-3428613 None:intergenic
CACATAATCAAGGGATGCAG+TGG 0.590826 2.4:+3428408 MS.gene85145:CDS
TTGCAGACAAGAGAATGTTA+AGG 0.593256 2.4:+3426049 MS.gene85145:CDS
TCAAATCTCTCACTTACTTG+AGG 0.596625 2.4:-3427921 None:intergenic
TTACATCTTGCAGTTTCAGG+TGG 0.598056 2.4:+3427216 MS.gene85145:CDS
GCTTTGCAATACAACAACAA+TGG 0.600689 2.4:+3426973 MS.gene85145:CDS
GCCATATTTATCAGAGCTGG+TGG 0.603349 2.4:+3427708 MS.gene85145:CDS
TTTCAAAGCTCTTACCTACA+GGG 0.604162 2.4:+3428599 MS.gene85145:CDS
AGAAAGCCTATAAGCTGGCG+AGG 0.604938 2.4:-3427787 None:intergenic
ATGAGAGGCAAATCGATGGT+TGG 0.608725 2.4:+3428182 MS.gene85145:CDS
ACCAATGAGAGGCAAATCGA+TGG 0.610064 2.4:+3428178 MS.gene85145:CDS
CAAAAGCATCATCATAACAA+AGG 0.610733 2.4:+3427993 MS.gene85145:CDS
TTAGGGATATATCTTCGCGA+TGG 0.611506 2.4:-3427810 None:intergenic
TGGCTATGTTGCAGCTACAT+GGG 0.618021 2.4:+3428376 MS.gene85145:CDS
CCTGGTGGTGTGTATCAAGA+AGG 0.618149 2.4:+3428155 MS.gene85145:CDS
CAAGTGGAGAAAGAAAAGAA+AGG 0.618316 2.4:+3428526 MS.gene85145:CDS
TTCCTGACACAAAATCATCA+AGG 0.620697 2.4:-3427041 None:intergenic
AGCATAGTGAAAGAAAGAGA+AGG 0.622329 2.4:+3425873 MS.gene85145:CDS
TGAGATTGTGGAGCCAGGAG+TGG 0.630155 2.4:+3426199 MS.gene85145:CDS
GCTGCATCAAGAGGCCACAC+AGG 0.631864 2.4:+3426251 MS.gene85145:CDS
CTATCATGAGATGCACAAAG+AGG 0.644135 2.4:+3428052 MS.gene85145:CDS
GAAAGCCTATAAGCTGGCGA+GGG 0.647143 2.4:-3427786 None:intergenic
TATCAAGAAGGACCAATGAG+AGG 0.648611 2.4:+3428167 MS.gene85145:CDS
TTACCAAGTCAAGATGGCCA+TGG 0.649460 2.4:-3426157 None:intergenic
CATGGAGACTCCAATTCATG+AGG 0.649543 2.4:+3426025 MS.gene85145:CDS
GTCTAGTTCAAGTGAAAACG+AGG 0.654066 2.4:-3427751 None:intergenic
TTCTGCTGAGAATAATAACA+TGG 0.655167 2.4:+3426007 MS.gene85145:CDS
TCTCAGCAGAAACCATGTCA+GGG 0.665903 2.4:-3425995 None:intergenic
TTAATACTAGGAATAGTGAA+GGG 0.667856 2.4:+3427625 MS.gene85145:CDS
ACCAGCAGCAAAAGTTACAG+TGG 0.670740 2.4:-3428123 None:intergenic
TCTTGATACACACCACCAGG+TGG 0.691486 2.4:-3428152 None:intergenic
AAACTCTATGCTCACCTGTG+TGG 0.693290 2.4:-3426265 None:intergenic
ACTTCCTTAGGAAATCCAAG+TGG 0.726512 2.4:+3428510 MS.gene85145:CDS
GCTGGTATTAGTCCACCTGG+TGG 0.740487 2.4:+3428140 MS.gene85145:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAATTAGATGGTTAAT+AGG + chr2.4:3426631-3426650 MS.gene85145:intron 15.0%
!! TTTAATCACTAATAATAAGA+AGG + chr2.4:3426745-3426764 MS.gene85145:intron 15.0%
!! AAAATACTGTTTGTAGTATA+TGG + chr2.4:3427484-3427503 MS.gene85145:intron 20.0%
!! ACGAAAAAATAAGTTCAAAA+AGG - chr2.4:3426335-3426354 None:intergenic 20.0%
!! ATGCAATTAAACAATATATC+AGG + chr2.4:3427459-3427478 MS.gene85145:intron 20.0%
!! CAATATCCAAGAATTATTTA+TGG + chr2.4:3427542-3427561 MS.gene85145:intron 20.0%
!! TAGTAATGAACTACTATTTA+GGG + chr2.4:3426354-3426373 MS.gene85145:intron 20.0%
!!! TTTTGTTTCACTAAATTTGT+TGG - chr2.4:3427978-3427997 None:intergenic 20.0%
! AAAATGTGAATCATACTAAG+AGG + chr2.4:3428024-3428043 MS.gene85145:CDS 25.0%
! AACAATAACTACAGATAAAG+AGG - chr2.4:3428261-3428280 None:intergenic 25.0%
! AAGATATATCCCTAATGAAA+TGG + chr2.4:3427818-3427837 MS.gene85145:CDS 25.0%
! ATGATCAACAATAATGTTTG+TGG - chr2.4:3428314-3428333 None:intergenic 25.0%
! ATTAATACTAGGAATAGTGA+AGG + chr2.4:3427624-3427643 MS.gene85145:CDS 25.0%
! CCAAGAATTATTTATGGTTA+TGG + chr2.4:3427548-3427567 MS.gene85145:intron 25.0%
! CCATAACCATAAATAATTCT+TGG - chr2.4:3427551-3427570 None:intergenic 25.0%
! CTATAGGGTTAAAAATTAGA+TGG + chr2.4:3426623-3426642 MS.gene85145:intron 25.0%
! GTAGTAATGAACTACTATTT+AGG + chr2.4:3426353-3426372 MS.gene85145:intron 25.0%
! TTAATACTAGGAATAGTGAA+GGG + chr2.4:3427625-3427644 MS.gene85145:CDS 25.0%
! TTTAGGGAGATATTAACTTA+AGG + chr2.4:3426370-3426389 MS.gene85145:intron 25.0%
!! CATCTAATTTTTAACCCTAT+AGG - chr2.4:3426625-3426644 None:intergenic 25.0%
!!! AAAAAAGCACTTTTACAACT+AGG - chr2.4:3426125-3426144 None:intergenic 25.0%
!!! AAGATTTTTCAAATGCTTAC+TGG + chr2.4:3427420-3427439 MS.gene85145:intron 25.0%
!!! TTGTAAAAGTGCTTTTTTTG+TGG + chr2.4:3426127-3426146 MS.gene85145:CDS 25.0%
AAAGAAAAGAAAGGAAAGTG+GGG + chr2.4:3428535-3428554 MS.gene85145:CDS 30.0%
AAGAAACACCATAGTATTAG+TGG + chr2.4:3428088-3428107 MS.gene85145:CDS 30.0%
AATATGAATGATAACCCTGT+AGG - chr2.4:3428616-3428635 None:intergenic 30.0%
ACTGTTGTAATTTGACTGAT+TGG + chr2.4:3427368-3427387 MS.gene85145:intron 30.0%
AGAAAGAAAAGAAAGGAAAG+TGG + chr2.4:3428533-3428552 MS.gene85145:CDS 30.0%
CAAAGCTGGATATTAATACT+AGG + chr2.4:3427613-3427632 MS.gene85145:CDS 30.0%
CACTAATAATAAGAAGGTGA+TGG + chr2.4:3426751-3426770 MS.gene85145:intron 30.0%
GAAAGAAAAGAAAGGAAAGT+GGG + chr2.4:3428534-3428553 MS.gene85145:CDS 30.0%
TTAGCACTTCCATTTCATTA+GGG - chr2.4:3427830-3427849 None:intergenic 30.0%
TTCTGCTGAGAATAATAACA+TGG + chr2.4:3426007-3426026 MS.gene85145:CDS 30.0%
TTGCACAATGTTAATATCTG+TGG + chr2.4:3426698-3426717 MS.gene85145:intron 30.0%
TTGCATCATGCTTGTAATAA+AGG + chr2.4:3426904-3426923 MS.gene85145:CDS 30.0%
TTTCACTATGCTTGAAAATG+TGG - chr2.4:3425865-3425884 None:intergenic 30.0%
TTTCATTTAGCTGTGAGATA+TGG + chr2.4:3427108-3427127 MS.gene85145:CDS 30.0%
! ATTGTGCAATTTTACTCCTT+AGG - chr2.4:3426687-3426706 None:intergenic 30.0%
! GTTTGTGGTTTTCTTCTAAA+TGG - chr2.4:3428299-3428318 None:intergenic 30.0%
! TGATTTTGCCACATAATCAA+GGG + chr2.4:3428399-3428418 MS.gene85145:CDS 30.0%
!! ATAATAAGAAGGTGATGGAT+TGG + chr2.4:3426756-3426775 MS.gene85145:intron 30.0%
!! CAAAAGCATCATCATAACAA+AGG + chr2.4:3427993-3428012 MS.gene85145:CDS 30.0%
!! CATTTGAATCACGTTTTAGT+AGG - chr2.4:3426951-3426970 None:intergenic 30.0%
ACCAATTTGGCAAACTGAAA+TGG - chr2.4:3426573-3426592 None:intergenic 35.0%
AGCATAGTGAAAGAAAGAGA+AGG + chr2.4:3425873-3425892 MS.gene85145:CDS 35.0%
AGGGAGATATTAACTTAAGG+CGG + chr2.4:3426373-3426392 MS.gene85145:intron 35.0%
ATATGGATGCTATGATGCTT+TGG + chr2.4:3427125-3427144 MS.gene85145:CDS 35.0%
ATTAGATGGTTAATAGGCAG+CGG + chr2.4:3426637-3426656 MS.gene85145:intron 35.0%
ATTGTTGATCATTGCTCACA+AGG + chr2.4:3428322-3428341 MS.gene85145:intron 35.0%
CAAAACAGCCACTAATACTA+TGG - chr2.4:3428099-3428118 None:intergenic 35.0%
CAAGTATGGTTGCATTGAAA+TGG + chr2.4:3425950-3425969 MS.gene85145:CDS 35.0%
CAAGTGGAGAAAGAAAAGAA+AGG + chr2.4:3428526-3428545 MS.gene85145:CDS 35.0%
CATTTCAATGCAACCATACT+TGG - chr2.4:3425952-3425971 None:intergenic 35.0%
CCATAAGGTGAGAAATTGAT+GGG + chr2.4:3427242-3427261 MS.gene85145:intron 35.0%
CTATGGACAGTGTAGAAAAT+AGG + chr2.4:3427676-3427695 MS.gene85145:CDS 35.0%
GCATAAACAGTCAACAGTTT+AGG - chr2.4:3426811-3426830 None:intergenic 35.0%
GCATCGTCTTATAGAAAAGT+CGG + chr2.4:3427343-3427362 MS.gene85145:intron 35.0%
GCTTTGCAATACAACAACAA+TGG + chr2.4:3426973-3426992 MS.gene85145:CDS 35.0%
GTAAGTGAGAGATTTGAGAA+TGG + chr2.4:3427927-3427946 MS.gene85145:CDS 35.0%
GTGTTTCGATTATGCTAGTT+AGG + chr2.4:3426417-3426436 MS.gene85145:intron 35.0%
GTTAGCACTTCCATTTCATT+AGG - chr2.4:3427831-3427850 None:intergenic 35.0%
TCAAATCTCTCACTTACTTG+AGG - chr2.4:3427924-3427943 None:intergenic 35.0%
TCTCAGATAGATTCAACAAG+AGG - chr2.4:3426184-3426203 None:intergenic 35.0%
TGTAGAAATTGAGATGCCTA+AGG + chr2.4:3426668-3426687 MS.gene85145:intron 35.0%
TTCCTGACACAAAATCATCA+AGG - chr2.4:3427044-3427063 None:intergenic 35.0%
TTGCAGACAAGAGAATGTTA+AGG + chr2.4:3426049-3426068 MS.gene85145:CDS 35.0%
TTGTTAGAGAGTTGTTGAAC+AGG + chr2.4:3426836-3426855 MS.gene85145:CDS 35.0%
TTTCAAAGCTCTTACCTACA+GGG + chr2.4:3428599-3428618 MS.gene85145:CDS 35.0%
TTTCTTATTGCTGCATCAAG+AGG + chr2.4:3426242-3426261 MS.gene85145:CDS 35.0%
! CGAGAAGTAGTATGGATTAT+AGG - chr2.4:3427955-3427974 None:intergenic 35.0%
! CTTTCTTTTCTTTCTCCACT+TGG - chr2.4:3428528-3428547 None:intergenic 35.0%
! CTTTTCCATTCATTACTGCA+AGG - chr2.4:3427014-3427033 None:intergenic 35.0%
! GTGATTTTGCCACATAATCA+AGG + chr2.4:3428398-3428417 MS.gene85145:CDS 35.0%
! GTTGAATCTATCTGAGATTG+TGG + chr2.4:3426187-3426206 MS.gene85145:CDS 35.0%
! TAAGGCGGAAATGTACTTTT+TGG + chr2.4:3426388-3426407 MS.gene85145:intron 35.0%
! TGGTAGAACATCAGCTTTTA+AGG + chr2.4:3428202-3428221 MS.gene85145:CDS 35.0%
! TTTTCAAAGCTCTTACCTAC+AGG + chr2.4:3428598-3428617 MS.gene85145:CDS 35.0%
!! AATGGAAGTGCTAACTGAAA+TGG + chr2.4:3427836-3427855 MS.gene85145:CDS 35.0%
!! ATCCTTGATGATTTTGTGTC+AGG + chr2.4:3427039-3427058 MS.gene85145:CDS 35.0%
!! TGACGTTGACTTAACCAATT+TGG - chr2.4:3426586-3426605 None:intergenic 35.0%
!!! CTATGATGCTTTGGTTTTCT+TGG + chr2.4:3427134-3427153 MS.gene85145:CDS 35.0%
AAAAGAAAGGAAAGTGGGGA+TGG + chr2.4:3428539-3428558 MS.gene85145:CDS 40.0%
AAGGATACAGAGAGACTGTA+TGG + chr2.4:3426923-3426942 MS.gene85145:CDS 40.0%
ACTTCCTTAGGAAATCCAAG+TGG + chr2.4:3428510-3428529 MS.gene85145:CDS 40.0%
AGAGGTTTACCAAGTCAAGA+TGG - chr2.4:3426166-3426185 None:intergenic 40.0%
AGCACTTGACATTCTTGATC+AGG + chr2.4:3427653-3427672 MS.gene85145:CDS 40.0%
ATCTATCTTGGCAGTGCAAT+AGG - chr2.4:3427182-3427201 None:intergenic 40.0%
CAAGCCATATTTATCAGAGC+TGG + chr2.4:3427705-3427724 MS.gene85145:CDS 40.0%
CAAGTGAAAACGAGGAAGAT+CGG - chr2.4:3427746-3427765 None:intergenic 40.0%
CACTGTTGCCATATCTATCT+TGG - chr2.4:3427194-3427213 None:intergenic 40.0%
CATTGCTCACAAGGTTATGT+GGG + chr2.4:3428331-3428350 MS.gene85145:intron 40.0%
CCCATCAATTTCTCACCTTA+TGG - chr2.4:3427245-3427264 None:intergenic 40.0%
CTATCATGAGATGCACAAAG+AGG + chr2.4:3428052-3428071 MS.gene85145:CDS 40.0%
CTTGATCAACAAGACAAAGC+TGG + chr2.4:3427599-3427618 MS.gene85145:CDS 40.0%
GCCATAAGGTGAGAAATTGA+TGG + chr2.4:3427241-3427260 MS.gene85145:intron 40.0%
GCCATTTCAGTTTGCCAAAT+TGG + chr2.4:3426569-3426588 MS.gene85145:intron 40.0%
GTCTTACATCTTGCAGTTTC+AGG + chr2.4:3427213-3427232 MS.gene85145:CDS 40.0%
GTGCAATAGGTTTGTACCAT+TGG - chr2.4:3427169-3427188 None:intergenic 40.0%
TAAGACTCACGTAGCCTATA+GGG + chr2.4:3426608-3426627 MS.gene85145:intron 40.0%
TAAGCAAGCTGATAGTTGTG+TGG + chr2.4:3426547-3426566 MS.gene85145:intron 40.0%
TACTTGGAAGCTAGGTGTAA+TGG - chr2.4:3425936-3425955 None:intergenic 40.0%
TATCAAGAAGGACCAATGAG+AGG + chr2.4:3428167-3428186 MS.gene85145:CDS 40.0%
TATCTCGATGTACGTTTCAC+AGG - chr2.4:3427298-3427317 None:intergenic 40.0%
TCATTGCTCACAAGGTTATG+TGG + chr2.4:3428330-3428349 MS.gene85145:intron 40.0%
TCGATCACTTGAGAAAGATC+TGG - chr2.4:3426865-3426884 None:intergenic 40.0%
TCTCTAGGCACAATCTTCAT+TGG + chr2.4:3428464-3428483 MS.gene85145:CDS 40.0%
TGACATTCTTGATCAGGCTA+TGG + chr2.4:3427659-3427678 MS.gene85145:CDS 40.0%
TGTTAGATTATGCCCTGACA+TGG + chr2.4:3425983-3426002 MS.gene85145:CDS 40.0%
TTACACCTAGCTTCCAAGTA+TGG + chr2.4:3425936-3425955 MS.gene85145:CDS 40.0%
TTACATCTTGCAGTTTCAGG+TGG + chr2.4:3427216-3427235 MS.gene85145:CDS 40.0%
TTAGAGAGTTGTTGAACAGG+TGG + chr2.4:3426839-3426858 MS.gene85145:CDS 40.0%
TTAGGGATATATCTTCGCGA+TGG - chr2.4:3427813-3427832 None:intergenic 40.0%
TTCATTACTGCAAGGTGCAA+TGG - chr2.4:3427006-3427025 None:intergenic 40.0%
TTCTCCACTTGGATTTCCTA+AGG - chr2.4:3428517-3428536 None:intergenic 40.0%
TTGCACCTTGCAGTAATGAA+TGG + chr2.4:3427006-3427025 MS.gene85145:CDS 40.0%
TTGCACTGCCAAGATAGATA+TGG + chr2.4:3427183-3427202 MS.gene85145:CDS 40.0%
TTTGTTGGCGAGAAGTAGTA+TGG - chr2.4:3427963-3427982 None:intergenic 40.0%
! ACACAACTATCAGCTTGCTT+AGG - chr2.4:3426548-3426567 None:intergenic 40.0%
! ACCACCAGCTCTGATAAATA+TGG - chr2.4:3427712-3427731 None:intergenic 40.0%
! GTCTAGTTCAAGTGAAAACG+AGG - chr2.4:3427754-3427773 None:intergenic 40.0%
! TTCTTGGTGCAAGTTTCCAA+TGG + chr2.4:3427150-3427169 MS.gene85145:CDS 40.0%
!! GCTTAAGTTGACACCAACTT+AGG - chr2.4:3426523-3426542 None:intergenic 40.0%
!! TAGGTGTAATGGAGTGTTGA+AGG - chr2.4:3425925-3425944 None:intergenic 40.0%
!! TGAAGGTATCATCTGTTCTC+TGG - chr2.4:3425908-3425927 None:intergenic 40.0%
!!! TTTTTTGTGGCTTGTAGCCA+TGG + chr2.4:3426140-3426159 MS.gene85145:CDS 40.0%
AAACTCTATGCTCACCTGTG+TGG - chr2.4:3426268-3426287 None:intergenic 45.0%
AACATAGCCAGTTGCCATGA+AGG - chr2.4:3428366-3428385 None:intergenic 45.0%
ACCAATGAGAGGCAAATCGA+TGG + chr2.4:3428178-3428197 MS.gene85145:CDS 45.0%
ACCAGCAGCAAAAGTTACAG+TGG - chr2.4:3428126-3428145 None:intergenic 45.0%
ATGAGAGGCAAATCGATGGT+TGG + chr2.4:3428182-3428201 MS.gene85145:CDS 45.0%
ATTAAGCTTGCAAGCAGCTG+TGG - chr2.4:3426102-3426121 None:intergenic 45.0%
CACATAATCAAGGGATGCAG+TGG + chr2.4:3428408-3428427 MS.gene85145:CDS 45.0%
CACTGCATCCCTTGATTATG+TGG - chr2.4:3428410-3428429 None:intergenic 45.0%
CATGGAGACTCCAATTCATG+AGG + chr2.4:3426025-3426044 MS.gene85145:CDS 45.0%
CTAAGACTCACGTAGCCTAT+AGG + chr2.4:3426607-3426626 MS.gene85145:intron 45.0%
CTCAAGTGATCGACGAGAAT+GGG + chr2.4:3426872-3426891 MS.gene85145:CDS 45.0%
GATGGAGAAAGCCTATAAGC+TGG - chr2.4:3427795-3427814 None:intergenic 45.0%
GCCATATTTATCAGAGCTGG+TGG + chr2.4:3427708-3427727 MS.gene85145:CDS 45.0%
TCTCAAGTGATCGACGAGAA+TGG + chr2.4:3426871-3426890 MS.gene85145:CDS 45.0%
TCTCAGCAGAAACCATGTCA+GGG - chr2.4:3425998-3426017 None:intergenic 45.0%
TGCAACCATACTTGGAAGCT+AGG - chr2.4:3425944-3425963 None:intergenic 45.0%
TGGAGCTGCTGAATCAGATA+AGG + chr2.4:3428559-3428578 MS.gene85145:CDS 45.0%
TGGCTATGTTGCAGCTACAT+GGG + chr2.4:3428376-3428395 MS.gene85145:CDS 45.0%
TGTCTGCAAGCCTCATGAAT+TGG - chr2.4:3426038-3426057 None:intergenic 45.0%
TTAAGCTTGCAAGCAGCTGT+GGG - chr2.4:3426101-3426120 None:intergenic 45.0%
TTACCAAGTCAAGATGGCCA+TGG - chr2.4:3426160-3426179 None:intergenic 45.0%
TTCTCAGCAGAAACCATGTC+AGG - chr2.4:3425999-3426018 None:intergenic 45.0%
! AGCTTTTGCTAACTGGTGGA+GGG - chr2.4:3427881-3427900 None:intergenic 45.0%
! CAACTATCAGCTTGCTTAGG+TGG - chr2.4:3426545-3426564 None:intergenic 45.0%
! GATTCGGAGCTTTTGCTAAC+TGG - chr2.4:3427888-3427907 None:intergenic 45.0%
! TCTGTTTTGCTGCTAGCACT+TGG + chr2.4:3428434-3428453 MS.gene85145:CDS 45.0%
! TGCTGGTTGAACACTTCCTT+AGG + chr2.4:3428498-3428517 MS.gene85145:CDS 45.0%
!! ACCATCGATTTGCCTCTCAT+TGG - chr2.4:3428182-3428201 None:intergenic 45.0%
!! CATTGGTCTTAGTGTGATGC+TGG + chr2.4:3428481-3428500 MS.gene85145:CDS 45.0%
AGAAAGCCTATAAGCTGGCG+AGG - chr2.4:3427790-3427809 None:intergenic 50.0%
AGGCTGAGGCGATCTAGATT+CGG - chr2.4:3427904-3427923 None:intergenic 50.0%
ATTCTCCCTCGCCAGCTTAT+AGG + chr2.4:3427781-3427800 MS.gene85145:CDS 50.0%
CAAGGTTATGTGGGTAGCTG+TGG + chr2.4:3428340-3428359 MS.gene85145:intron 50.0%
CCTTCTTGATACACACCACC+AGG - chr2.4:3428158-3428177 None:intergenic 50.0%
CTCTCACTTACTTGAGGCTG+AGG - chr2.4:3427918-3427937 None:intergenic 50.0%
CTGGCTATGTTGCAGCTACA+TGG + chr2.4:3428375-3428394 MS.gene85145:CDS 50.0%
GAAAGCCTATAAGCTGGCGA+GGG - chr2.4:3427789-3427808 None:intergenic 50.0%
TAGCCATGGCCATCTTGACT+TGG + chr2.4:3426154-3426173 MS.gene85145:CDS 50.0%
TCTTGATACACACCACCAGG+TGG - chr2.4:3428155-3428174 None:intergenic 50.0%
TTAACGCCTGAAGCCTACGT+GGG + chr2.4:3426497-3426516 MS.gene85145:intron 50.0%
TTTAACGCCTGAAGCCTACG+TGG + chr2.4:3426496-3426515 MS.gene85145:intron 50.0%
! AGTTTCAGGTGGTCGCCATA+AGG + chr2.4:3427227-3427246 MS.gene85145:CDS 50.0%
! CCTGGTGGTGTGTATCAAGA+AGG + chr2.4:3428155-3428174 MS.gene85145:CDS 50.0%
! CTATCTGAGATTGTGGAGCC+AGG + chr2.4:3426194-3426213 MS.gene85145:CDS 50.0%
! GAGCTTTTGCTAACTGGTGG+AGG - chr2.4:3427882-3427901 None:intergenic 50.0%
! GCCACTGTAACTTTTGCTGC+TGG + chr2.4:3428122-3428141 MS.gene85145:CDS 50.0%
! GTTTTGCTGCTAGCACTTGG+TGG + chr2.4:3428437-3428456 MS.gene85145:CDS 50.0%
! TCGGAGCTTTTGCTAACTGG+TGG - chr2.4:3427885-3427904 None:intergenic 50.0%
AAGCCTACGTGGGCCTAAGT+TGG + chr2.4:3426507-3426526 MS.gene85145:intron 55.0%
ACACCAACTTAGGCCCACGT+AGG - chr2.4:3426513-3426532 None:intergenic 55.0%
GCTGCTGGTATTAGTCCACC+TGG + chr2.4:3428137-3428156 MS.gene85145:CDS 55.0%
GCTGGTATTAGTCCACCTGG+TGG + chr2.4:3428140-3428159 MS.gene85145:CDS 55.0%
TGAGATTGTGGAGCCAGGAG+TGG + chr2.4:3426199-3426218 MS.gene85145:CDS 55.0%
TGATCAAACCCTGCCACTCC+TGG - chr2.4:3426215-3426234 None:intergenic 55.0%
! TTGCTGCTAGCACTTGGTGG+TGG + chr2.4:3428440-3428459 MS.gene85145:CDS 55.0%
ATTGTGGAGCCAGGAGTGGC+AGG + chr2.4:3426203-3426222 MS.gene85145:CDS 60.0%
CTTAGGCCCACGTAGGCTTC+AGG - chr2.4:3426506-3426525 None:intergenic 60.0%
GCTGCATCAAGAGGCCACAC+AGG + chr2.4:3426251-3426270 MS.gene85145:CDS 60.0%
GCTGTGGCCTTCATGGCAAC+TGG + chr2.4:3428356-3428375 MS.gene85145:CDS 60.0%
GTGGGTAGCTGTGGCCTTCA+TGG + chr2.4:3428349-3428368 MS.gene85145:CDS 60.0%
TTGTGGAGCCAGGAGTGGCA+GGG + chr2.4:3426204-3426223 MS.gene85145:CDS 60.0%
!! GCACTTGGTGGTGGCTCTCT+AGG + chr2.4:3428449-3428468 MS.gene85145:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 3425819 3428637 3425819 ID=MS.gene85145
chr2.4 mRNA 3425819 3428637 3425819 ID=MS.gene85145.t1;Parent=MS.gene85145
chr2.4 exon 3425819 3426272 3425819 ID=MS.gene85145.t1.exon1;Parent=MS.gene85145.t1
chr2.4 CDS 3425819 3426272 3425819 ID=cds.MS.gene85145.t1;Parent=MS.gene85145.t1
chr2.4 exon 3426833 3427248 3426833 ID=MS.gene85145.t1.exon2;Parent=MS.gene85145.t1
chr2.4 CDS 3426833 3427248 3426833 ID=cds.MS.gene85145.t1;Parent=MS.gene85145.t1
chr2.4 exon 3427588 3428223 3427588 ID=MS.gene85145.t1.exon3;Parent=MS.gene85145.t1
chr2.4 CDS 3427588 3428223 3427588 ID=cds.MS.gene85145.t1;Parent=MS.gene85145.t1
chr2.4 exon 3428344 3428637 3428344 ID=MS.gene85145.t1.exon4;Parent=MS.gene85145.t1
chr2.4 CDS 3428344 3428637 3428344 ID=cds.MS.gene85145.t1;Parent=MS.gene85145.t1
Gene Sequence

>MS.gene85145

ATGGATCAAGAGTTTTTTAATGCAATTAAGAATAATGACATTTCCACATTTTCAAGCATAGTGAAAGAAAGAGAAGGAATTCTCAACCAGAGAACAGATGATACCTTCAACACTCCATTACACCTAGCTTCCAAGTATGGTTGCATTGAAATGGTATCTGAGATTGTTAGATTATGCCCTGACATGGTTTCTGCTGAGAATAATAACATGGAGACTCCAATTCATGAGGCTTGCAGACAAGAGAATGTTAAGGTCTTAATGTTGCTTTTAGAAGTCAATCCCACAGCTGCTTGCAAGCTTAATCCTAGTTGTAAAAGTGCTTTTTTTGTGGCTTGTAGCCATGGCCATCTTGACTTGGTAAACCTCTTGTTGAATCTATCTGAGATTGTGGAGCCAGGAGTGGCAGGGTTTGATCAAGCTTGTTTTCTTATTGCTGCATCAAGAGGCCACACAGGTGAGCATAGAGTTTTCTTTACTATGCTTGCATTTTTTTTAACTCTAATATCAAATTTACCTTTTTGAACTTATTTTTTCGTAGTAATGAACTACTATTTAGGGAGATATTAACTTAAGGCGGAAATGTACTTTTTGGTAGATTGTGTTTCGATTATGCTAGTTAGGTAGACATGTAATCTAATTGATACAATCAGATTACACTGTTAATTAAACGTAAAAGTTTTAACGCCTGAAGCCTACGTGGGCCTAAGTTGGTGTCAACTTAAGCCACCTAAGCAAGCTGATAGTTGTGTGGCCATTTCAGTTTGCCAAATTGGTTAAGTCAACGTCAACTAAGACTCACGTAGCCTATAGGGTTAAAAATTAGATGGTTAATAGGCAGCGGAATTTGATTGTAGAAATTGAGATGCCTAAGGAGTAAAATTGCACAATGTTAATATCTGTGGACTAAAAATGAAATTAAGCTCAACTTTAATCACTAATAATAAGAAGGTGATGGATTGGAAATTATATATAGTTGATGTAAGATATATCCTAAACTGTTGACTGTTTATGCAGATATTGTTAGAGAGTTGTTGAACAGGTGGCCAGATCTTTCTCAAGTGATCGACGAGAATGGGAATTCAGCATTGCATCATGCTTGTAATAAAGGATACAGAGAGACTGTATGGATCCTACTAAAACGTGATTCAAATGTTGCTTTGCAATACAACAACAATGGTTACACACCATTGCACCTTGCAGTAATGAATGGAAAAGTATCGATCCTTGATGATTTTGTGTCAGGAAGTGCTGCATCATTTCATTATCTCACAAGAGAAGAAGAGACAGTATTTCATTTAGCTGTGAGATATGGATGCTATGATGCTTTGGTTTTCTTGGTGCAAGTTTCCAATGGTACAAACCTATTGCACTGCCAAGATAGATATGGCAACAGTGTCTTACATCTTGCAGTTTCAGGTGGTCGCCATAAGGTGAGAAATTGATGGGAGTTCTAAACTTATTTAACTATTGTTTTAACCTGTGAAACGTACATCGAGATAATTTTAGTTTATTTATATTCATATTGCATCGTCTTATAGAAAAGTCGGTAACTGTTGTAATTTGACTGATTGGAATGATGTAAAAAACAAAAAATAACAATTAAGATTTTTCAAATGCTTACTGGTTTGAAATCATGATAAATGCAATTAAACAATATATCAGGCAAAAATACTGTTTGTAGTATATGGCTTTAGTACATATTCTTTCTCAATTTTATTTTGTTCAATATCCAAGAATTATTTATGGTTATGGTTTTGTGATAATTGTAGATGACAGATTTCTTGATCAACAAGACAAAGCTGGATATTAATACTAGGAATAGTGAAGGGATGACAGCACTTGACATTCTTGATCAGGCTATGGACAGTGTAGAAAATAGGCAACTACAAGCCATATTTATCAGAGCTGGTGGTAAAAGAAGCATCCGATCTTCCTCGTTTTCACTTGAACTAGACAAGAACAATTCTCCCTCGCCAGCTTATAGGCTTTCTCCATCGCGAAGATATATCCCTAATGAAATGGAAGTGCTAACTGAAATGGTTTCTTATGATTGTATAAGCCCTCCACCAGTTAGCAAAAGCTCCGAATCTAGATCGCCTCAGCCTCAAGTAAGTGAGAGATTTGAGAATGGAACCTATAATCCATACTACTTCTCGCCAACAAATTTAGTGAAACAAAAGCATCATCATAACAAAGGAAAAATTGAAAATGTGAATCATACTAAGAGGAAACACTATCATGAGATGCACAAAGAGGCATTACTAAATGCAAGAAACACCATAGTATTAGTGGCTGTTTTGATTGCCACTGTAACTTTTGCTGCTGGTATTAGTCCACCTGGTGGTGTGTATCAAGAAGGACCAATGAGAGGCAAATCGATGGTTGGTAGAACATCAGCTTTTAAGGTATTTGCAATAAGCAATAACATAGCACTCTTCACCTCTTTATCTGTAGTTATTGTTCTTGTTAGTGTCATTCCATTTAGAAGAAAACCACAAACATTATTGTTGATCATTGCTCACAAGGTTATGTGGGTAGCTGTGGCCTTCATGGCAACTGGCTATGTTGCAGCTACATGGGTGATTTTGCCACATAATCAAGGGATGCAGTGGCTATCTGTTTTGCTGCTAGCACTTGGTGGTGGCTCTCTAGGCACAATCTTCATTGGTCTTAGTGTGATGCTGGTTGAACACTTCCTTAGGAAATCCAAGTGGAGAAAGAAAAGAAAGGAAAGTGGGGATGGAGCTGCTGAATCAGATAAGGAAAGTGAAAATTCAGATTTTCAAAGCTCTTACCTACAGGGTTATCATTCATATTGA
Protein sequence

>MS.gene85145.t1

MDQEFFNAIKNNDISTFSSIVKEREGILNQRTDDTFNTPLHLASKYGCIEMVSEIVRLCPDMVSAENNNMETPIHEACRQENVKVLMLLLEVNPTAACKLNPSCKSAFFVACSHGHLDLVNLLLNLSEIVEPGVAGFDQACFLIAASRGHTDIVRELLNRWPDLSQVIDENGNSALHHACNKGYRETVWILLKRDSNVALQYNNNGYTPLHLAVMNGKVSILDDFVSGSAASFHYLTREEETVFHLAVRYGCYDALVFLVQVSNGTNLLHCQDRYGNSVLHLAVSGGRHKMTDFLINKTKLDINTRNSEGMTALDILDQAMDSVENRQLQAIFIRAGGKRSIRSSSFSLELDKNNSPSPAYRLSPSRRYIPNEMEVLTEMVSYDCISPPPVSKSSESRSPQPQVSERFENGTYNPYYFSPTNLVKQKHHHNKGKIENVNHTKRKHYHEMHKEALLNARNTIVLVAVLIATVTFAAGISPPGGVYQEGPMRGKSMVGRTSAFKVMWVAVAFMATGYVAATWVILPHNQGMQWLSVLLLALGGGSLGTIFIGLSVMLVEHFLRKSKWRKKRKESGDGAAESDKESENSDFQSSYLQGYHSY