AlfalfaGEDB | MS.gene36348

M. sativa cultivar XinJiangDaYe / MS.gene36348

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00023.33 PF12796.10 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36348.t1	XP_003597696.1	76.1	639	92	3	1	579	1	638	7.00E-127	464.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36348.t1	A2CIR5	32.1	140	85	3	392	527	405	538	4.2e-11	71.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36348.t1	G7IUM3	76.1	639	92	3	1	579	1	638	5.1e-127	464.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene82413	MS.gene36348	PPI
MS.gene36348	MS.gene067580	PPI
MS.gene36348	MS.gene000074	PPI
MS.gene36348	MS.gene93910	PPI
MS.gene36348	MS.gene69642	PPI
MS.gene027995	MS.gene36348	PPI
MS.gene36348	MS.gene069900	PPI
MS.gene004294	MS.gene36348	PPI
MS.gene042083	MS.gene36348	PPI
MS.gene36348	MS.gene000639	PPI
MS.gene36348	MS.gene42371	PPI
MS.gene000633	MS.gene36348	PPI
MS.gene36348	MS.gene95095	PPI
MS.gene36348	MS.gene046976	PPI
MS.gene93914	MS.gene36348	PPI
MS.gene029949	MS.gene36348	PPI
MS.gene69634	MS.gene36348	PPI
MS.gene36348	MS.gene86533	PPI
MS.gene027986	MS.gene36348	PPI
MS.gene35272	MS.gene36348	PPI
MS.gene36348	MS.gene000628	PPI
MS.gene36348	MS.gene62818	PPI
MS.gene36348	MS.gene003142	PPI
MS.gene36348	MS.gene76567	PPI
MS.gene69643	MS.gene36348	PPI
MS.gene36348	MS.gene03114	PPI
MS.gene36348	MS.gene070627	PPI
MS.gene004696	MS.gene36348	PPI
MS.gene79559	MS.gene36348	PPI
MS.gene36348	MS.gene36385	PPI
MS.gene36348	MS.gene027991	PPI
MS.gene36348	MS.gene061578	PPI
MS.gene36348	MS.gene98466	PPI
MS.gene78960	MS.gene36348	PPI
MS.gene36348	MS.gene72664	PPI
MS.gene36348	MS.gene37152	PPI
MS.gene36348	MS.gene002615	PPI
MS.gene69614	MS.gene36348	PPI
MS.gene36348	MS.gene85179	PPI
MS.gene36348	MS.gene045052	PPI
MS.gene027978	MS.gene36348	PPI
MS.gene36348	MS.gene046978	PPI
MS.gene01949	MS.gene36348	PPI
MS.gene36348	MS.gene98456	PPI
MS.gene021743	MS.gene36348	PPI
MS.gene36348	MS.gene86539	PPI
MS.gene36348	MS.gene98479	PPI
MS.gene36348	MS.gene000079	PPI
MS.gene36348	MS.gene027972	PPI
MS.gene36348	MS.gene05028	PPI
MS.gene36348	MS.gene000075	PPI
MS.gene72910	MS.gene36348	PPI
MS.gene36348	MS.gene043157	PPI
MS.gene82979	MS.gene36348	PPI
MS.gene36348	MS.gene000081	PPI
MS.gene045049	MS.gene36348	PPI
MS.gene79563	MS.gene36348	PPI
MS.gene40201	MS.gene36348	PPI
MS.gene36348	MS.gene049681	PPI
MS.gene36348	MS.gene62721	PPI
MS.gene36348	MS.gene046977	PPI
MS.gene36348	MS.gene008350	PPI
MS.gene36348	MS.gene48617	PPI
MS.gene64896	MS.gene36348	PPI
MS.gene36348	MS.gene98458	PPI
MS.gene36348	MS.gene32079	PPI
MS.gene36348	MS.gene033400	PPI
MS.gene36348	MS.gene79561	PPI
MS.gene36348	MS.gene036251	PPI
MS.gene048265	MS.gene36348	PPI
MS.gene018224	MS.gene36348	PPI
MS.gene046852	MS.gene36348	PPI
MS.gene36348	MS.gene26538	PPI
MS.gene000078	MS.gene36348	PPI
MS.gene000644	MS.gene36348	PPI
MS.gene36348	MS.gene008759	PPI
MS.gene36348	MS.gene98470	PPI
MS.gene36348	MS.gene070630	PPI
MS.gene006878	MS.gene36348	PPI
MS.gene36348	MS.gene65337	PPI
MS.gene011447	MS.gene36348	PPI
MS.gene36348	MS.gene47485	PPI
MS.gene36348	MS.gene37227	PPI
MS.gene36348	MS.gene94378	PPI
MS.gene94380	MS.gene36348	PPI
MS.gene98484	MS.gene36348	PPI
MS.gene36348	MS.gene000646	PPI
MS.gene69111	MS.gene36348	PPI
MS.gene36348	MS.gene057689	PPI
MS.gene36348	MS.gene014559	PPI
MS.gene36348	MS.gene000642	PPI
MS.gene36348	MS.gene39617	PPI
MS.gene36348	MS.gene82412	PPI
MS.gene36348	MS.gene02894	PPI
MS.gene36348	MS.gene40013	PPI
MS.gene36348	MS.gene01601	PPI
MS.gene36348	MS.gene69639	PPI
MS.gene36348	MS.gene93912	PPI
MS.gene36348	MS.gene012743	PPI
MS.gene36348	MS.gene98475	PPI
MS.gene36348	MS.gene56444	PPI
MS.gene36348	MS.gene43431	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36348.t1	MTR_2g101320	75.430	639	97	3	1	579	1	639	0.0	873
MS.gene36348.t1	MTR_2g101310	69.111	641	133	10	1	579	1	638	0.0	827
MS.gene36348.t1	MTR_2g101310	79.125	503	44	4	1	443	1	502	0.0	775
MS.gene36348.t1	MTR_1g032100	24.746	493	334	9	101	572	53	529	7.83e-46	169
MS.gene36348.t1	MTR_1g032100	26.267	217	157	3	33	247	53	268	5.56e-15	78.2
MS.gene36348.t1	MTR_7g117620	27.969	522	341	13	68	572	41	544	1.96e-44	166
MS.gene36348.t1	MTR_7g117620	32.468	231	154	2	38	267	83	312	1.45e-22	102
MS.gene36348.t1	MTR_2g096190	27.695	538	336	16	68	572	25	542	1.71e-43	163
MS.gene36348.t1	MTR_2g096190	27.155	232	166	2	31	260	61	291	1.43e-22	102
MS.gene36348.t1	MTR_1g115575	26.538	520	344	10	38	550	75	563	3.18e-43	163
MS.gene36348.t1	MTR_1g115575	25.000	248	185	1	1	247	96	343	6.30e-23	103
MS.gene36348.t1	MTR_8g105260	26.636	428	287	9	138	553	98	510	9.86e-41	155
MS.gene36348.t1	MTR_8g105260	27.189	217	155	3	33	247	60	275	6.98e-17	84.3
MS.gene36348.t1	MTR_4g062100	26.969	508	329	13	68	551	29	518	9.55e-40	152
MS.gene36348.t1	MTR_4g062100	24.206	252	150	5	2	213	32	282	7.14e-17	84.3
MS.gene36348.t1	MTR_7g100390	26.946	501	326	13	71	549	11	493	3.24e-39	150
MS.gene36348.t1	MTR_7g100390	26.996	263	187	2	2	259	11	273	5.66e-24	106
MS.gene36348.t1	MTR_7g100390	29.268	164	116	0	33	196	115	278	2.52e-13	73.2
MS.gene36348.t1	MTR_7g100430	27.664	488	301	12	99	564	118	575	5.42e-38	148
MS.gene36348.t1	MTR_7g100430	28.977	176	123	2	39	213	161	335	7.09e-12	68.6
MS.gene36348.t1	MTR_1g069110	26.757	441	250	11	134	549	46	438	6.54e-25	108
MS.gene36348.t1	MTR_8g069880	24.107	448	264	14	133	550	34	435	9.98e-25	107
MS.gene36348.t1	MTR_7g021090	25.382	327	200	8	111	425	12	306	1.13e-23	103
MS.gene36348.t1	MTR_8g062410	24.350	423	256	9	133	533	37	417	1.22e-23	104
MS.gene36348.t1	MTR_8g072510	22.912	467	285	14	111	548	13	433	3.58e-23	103
MS.gene36348.t1	MTR_8g066670	23.810	399	245	8	96	463	1063	1433	8.59e-23	103
MS.gene36348.t1	MTR_8g073260	26.829	328	202	7	110	425	13	314	7.11e-22	99.0
MS.gene36348.t1	MTR_8g039620	24.234	359	222	8	133	464	41	376	4.50e-21	96.7
MS.gene36348.t1	MTR_7g099570	30.508	236	149	6	137	361	40	271	5.92e-21	97.4
MS.gene36348.t1	MTR_7g099570	25.366	205	133	6	40	229	9	208	8.29e-11	65.1
MS.gene36348.t1	MTR_3g105650	25.378	331	201	9	111	422	8	311	8.37e-20	92.4
MS.gene36348.t1	MTR_3g016210	25.566	309	185	7	137	425	41	324	4.33e-19	90.5
MS.gene36348.t1	MTR_1g059930	26.333	300	181	7	137	424	43	314	3.76e-18	87.4
MS.gene36348.t1	MTR_8g467780	25.484	310	183	6	137	425	42	324	1.24e-17	86.3
MS.gene36348.t1	MTR_8g040930	24.277	346	202	11	149	462	2	319	2.34e-17	84.7
MS.gene36348.t1	MTR_8g041240	24.277	346	202	11	149	462	2	319	2.34e-17	84.7
MS.gene36348.t1	MTR_3g085830	28.889	270	154	8	140	395	218	463	9.26e-17	84.3
MS.gene36348.t1	MTR_7g451420	24.925	333	202	10	110	425	11	312	4.43e-16	80.1
MS.gene36348.t1	MTR_8g068990	22.193	383	213	12	137	479	43	380	6.66e-16	80.9
MS.gene36348.t1	MTR_5g085480	22.746	488	290	17	100	535	206	658	6.97e-16	81.6
MS.gene36348.t1	MTR_5g085480	22.634	486	293	17	100	535	46	498	7.14e-16	81.3
MS.gene36348.t1	MTR_8g080080	24.919	309	189	9	40	328	106	391	5.28e-15	78.6
MS.gene36348.t1	MTR_5g085830	21.394	416	286	9	145	536	282	680	1.35e-14	77.4
MS.gene36348.t1	MTR_5g043640	31.148	183	116	7	101	280	51	226	1.94e-12	67.8
MS.gene36348.t1	MTR_7g099580	29.268	164	103	4	137	290	43	203	8.26e-12	67.4
MS.gene36348.t1	MTR_5g085810	22.693	401	263	12	145	517	308	689	1.22e-11	68.2
MS.gene36348.t1	MTR_6g027840	28.374	289	172	10	37	318	211	471	1.31e-11	67.8
MS.gene36348.t1	MTR_2g033050	26.087	230	150	7	100	317	161	382	1.89e-11	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36348.t1	AT1G10340	34.926	607	335	13	3	579	2	578	7.23e-105	328
MS.gene36348.t1	AT1G10340	34.596	607	333	13	3	579	2	574	4.35e-103	323
MS.gene36348.t1	AT1G34050	34.483	551	325	6	1	542	17	540	4.21e-99	313
MS.gene36348.t1	AT2G24600	34.355	620	340	15	5	579	4	601	3.33e-91	293
MS.gene36348.t1	AT2G24600	34.355	620	340	15	5	579	4	601	3.33e-91	293
MS.gene36348.t1	AT5G54710	33.271	532	319	8	33	549	65	575	2.04e-89	288
MS.gene36348.t1	AT5G54710	32.601	546	332	8	33	563	110	634	1.48e-88	287
MS.gene36348.t1	AT5G54710	23.611	288	166	5	54	287	2	289	1.04e-15	80.9
MS.gene36348.t1	AT5G50140	31.319	546	277	11	115	579	7	535	1.41e-63	218
MS.gene36348.t1	AT5G54700	35.694	353	178	5	192	542	17	322	9.06e-61	205
MS.gene36348.t1	AT2G24600	28.871	620	321	16	5	579	4	548	3.46e-57	201
MS.gene36348.t1	AT2G24600	28.871	620	321	16	5	579	4	548	3.46e-57	201
MS.gene36348.t1	AT5G54700	32.474	388	175	9	146	512	8	329	2.13e-48	176
MS.gene36348.t1	AT2G31820	28.030	528	316	13	38	550	153	631	4.96e-46	172
MS.gene36348.t1	AT2G01680	24.464	466	320	9	101	551	56	504	1.94e-45	168
MS.gene36348.t1	AT2G01680	21.154	260	190	3	3	247	12	271	1.47e-12	70.9
MS.gene36348.t1	AT1G05640	27.462	528	322	14	38	550	115	596	1.45e-43	165
MS.gene36348.t1	AT5G60070	28.125	480	297	13	99	552	68	525	2.63e-40	154
MS.gene36348.t1	AT5G60070	30.811	185	119	5	39	217	111	292	7.86e-13	71.6
MS.gene36348.t1	AT3G12360	25.505	545	357	12	38	571	64	570	5.76e-40	154
MS.gene36348.t1	AT3G12360	26.199	271	171	5	2	247	63	329	2.16e-20	95.5
MS.gene36348.t1	AT3G12360	25.505	545	357	12	38	571	76	582	6.40e-40	154
MS.gene36348.t1	AT3G12360	26.199	271	171	5	2	247	75	341	2.32e-20	95.5
MS.gene36348.t1	AT3G09550	27.959	490	275	11	38	496	90	532	2.29e-39	152
MS.gene36348.t1	AT3G09550	27.959	490	275	11	38	496	90	532	2.29e-39	152
MS.gene36348.t1	AT3G09550	27.959	490	275	11	38	496	97	539	2.50e-39	152
MS.gene36348.t1	AT1G07710	26.288	563	338	14	13	552	6	514	3.29e-38	148
MS.gene36348.t1	AT1G07710	26.288	563	338	14	13	552	6	514	3.29e-38	148
MS.gene36348.t1	AT5G02620	28.000	525	328	18	68	566	15	515	3.37e-36	142
MS.gene36348.t1	AT5G02620	28.000	525	328	18	68	566	15	515	3.37e-36	142
MS.gene36348.t1	AT5G02620	28.000	525	328	18	68	566	15	515	3.37e-36	142
MS.gene36348.t1	AT5G02620	25.557	583	354	19	2	566	18	538	1.15e-34	138
MS.gene36348.t1	AT5G02620	25.557	583	354	19	2	566	18	538	1.15e-34	138
MS.gene36348.t1	AT1G03670	24.903	518	323	12	38	534	108	580	7.24e-34	136
MS.gene36348.t1	AT4G03500	23.846	520	334	12	20	520	121	597	1.45e-31	130
MS.gene36348.t1	AT4G03500	23.846	520	334	12	20	520	98	574	2.14e-31	129
MS.gene36348.t1	AT4G03500	23.846	520	334	12	20	520	98	574	2.14e-31	129
MS.gene36348.t1	AT4G03450	23.946	522	306	11	1	463	36	525	1.27e-27	117
MS.gene36348.t1	AT4G03450	22.783	575	342	13	1	512	36	571	2.26e-27	117
MS.gene36348.t1	AT4G03460	22.814	469	297	10	20	463	67	495	4.79e-25	110
MS.gene36348.t1	AT4G03460	22.814	469	297	10	20	463	99	527	7.08e-25	109
MS.gene36348.t1	AT4G10720	27.273	374	221	10	137	491	36	377	6.57e-24	104
MS.gene36348.t1	AT4G10720	28.477	302	184	6	137	426	36	317	1.01e-23	104
MS.gene36348.t1	AT4G03440	22.321	560	324	16	39	524	155	677	3.60e-22	101
MS.gene36348.t1	AT4G03440	22.321	560	324	16	39	524	155	677	3.60e-22	101
MS.gene36348.t1	AT4G03440	22.321	560	324	16	39	524	162	684	3.67e-22	101
MS.gene36348.t1	AT4G14390	23.185	496	273	8	34	463	111	564	4.40e-22	101
MS.gene36348.t1	AT4G14390	23.185	496	273	8	34	463	130	583	4.52e-22	101
MS.gene36348.t1	AT5G51160	25.490	357	217	10	168	489	43	385	1.02e-21	98.6
MS.gene36348.t1	AT5G15500	28.144	334	200	7	111	427	9	319	1.95e-21	97.8
MS.gene36348.t1	AT4G14390	23.139	497	273	9	34	463	130	584	8.65e-21	97.1
MS.gene36348.t1	AT4G03480	24.173	393	245	10	95	463	293	656	2.31e-20	95.5
MS.gene36348.t1	AT4G03480	24.173	393	245	10	95	463	296	659	2.94e-20	95.5
MS.gene36348.t1	AT4G03470	23.132	562	335	13	33	539	116	635	9.48e-20	94.0
MS.gene36348.t1	AT1G14500	26.301	346	212	7	137	465	36	355	1.73e-18	88.6
MS.gene36348.t1	AT1G14480	24.709	344	225	5	137	465	36	360	4.02e-18	87.8
MS.gene36348.t1	AT5G54610	28.696	230	155	2	118	338	16	245	1.20e-17	85.9
MS.gene36348.t1	AT4G14390	25.000	348	192	5	34	319	111	451	3.97e-17	85.1
MS.gene36348.t1	AT5G54620	27.542	236	159	2	115	338	12	247	5.47e-17	84.0
MS.gene36348.t1	AT3G01750	28.477	302	170	10	40	319	109	386	1.14e-15	80.9
MS.gene36348.t1	AT3G01750	28.477	302	170	10	40	319	109	386	1.14e-15	80.9
MS.gene36348.t1	AT4G05040	22.449	490	284	11	34	463	113	566	1.37e-15	80.5
MS.gene36348.t1	AT4G05040	22.449	490	284	11	34	463	113	566	1.37e-15	80.5
MS.gene36348.t1	AT4G05040	22.449	490	284	11	34	463	113	566	1.37e-15	80.5
MS.gene36348.t1	AT4G05040	22.449	490	284	11	34	463	113	566	1.37e-15	80.5
MS.gene36348.t1	AT4G05040	22.449	490	284	11	34	463	113	566	1.37e-15	80.5
MS.gene36348.t1	AT4G05040	22.449	490	284	11	34	463	113	566	1.37e-15	80.5
MS.gene36348.t1	AT4G05040	22.449	490	284	11	34	463	113	566	1.37e-15	80.5
MS.gene36348.t1	AT3G04140	26.480	321	183	9	40	335	106	398	1.44e-15	80.5
MS.gene36348.t1	AT4G03490	24.806	258	186	2	61	315	135	387	5.92e-13	72.0
MS.gene36348.t1	AT4G03490	24.806	258	186	2	61	315	135	387	6.30e-13	72.0
MS.gene36348.t1	AT4G03490	24.806	258	186	2	61	315	125	377	6.43e-13	72.0
MS.gene36348.t1	AT1G14480	22.849	337	197	5	137	465	36	317	5.02e-12	68.6

Find 108 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCTTACATCTCGCAGTTTC+AGG	0.192386	2.2:+2213568	MS.gene36348:CDS
ATTGCATCTTGCAGTGATTA+AGG	0.228117	2.2:+2213360	MS.gene36348:CDS
GCACTTGGTGGTGGCTTTCT+AGG	0.247558	2.2:+2214789	MS.gene36348:CDS
TTGCATCATGCTTGTTATAA+AGG	0.260488	2.2:+2213259	MS.gene36348:CDS
TTACACCTTGCTTCCAAGTA+TGG	0.266322	2.2:+2212381	MS.gene36348:CDS
TTTGTAGATGGCAGATTTCT+TGG	0.266742	2.2:+2213928	MS.gene36348:intron
TACCTCTCATTGGTCCTTCT+TGG	0.285786	2.2:-2214509	None:intergenic
CATTTCAATGCAACCATACT+TGG	0.318111	2.2:-2212394	None:intergenic
ACTATTGACCATTGCTCATA+AGG	0.354887	2.2:+2214662	MS.gene36348:CDS
CTTCATCTTGCTGTGAGATA+TGG	0.376954	2.2:+2213463	MS.gene36348:CDS
TGTCTACAAGCCTCATGAAT+TGG	0.377283	2.2:-2212480	None:intergenic
AATGGAAGTGCTTAGTGAAA+TGG	0.384418	2.2:+2214180	MS.gene36348:CDS
AAGGTATACACCGAAGGAAA+TGG	0.384985	2.2:+2214162	MS.gene36348:CDS
ACTGTCTCTTCTTCTCTTGT+GGG	0.398158	2.2:-2213439	None:intergenic
TACTGTCTCTTCTTCTCTTG+TGG	0.399548	2.2:-2213440	None:intergenic
TGGCAGATTTCTTGGTCAAC+AGG	0.404227	2.2:+2213936	MS.gene36348:CDS
GCTGTGGCATTTATGTCAAC+TGG	0.408424	2.2:+2214696	MS.gene36348:CDS
TTGCATCTTGCAGTGATTAA+GGG	0.419674	2.2:+2213361	MS.gene36348:CDS
CTAAGCACTTCCATTTCCTT+CGG	0.423658	2.2:-2214172	None:intergenic
TTCCATGTTGCTGCAGTAAG+AGG	0.430756	2.2:+2212687	MS.gene36348:CDS
CATTGCATCTCTTGATTATG+TGG	0.432210	2.2:-2214747	None:intergenic
TTCTCAGCAGAAACCATGTC+AGG	0.439348	2.2:-2212441	None:intergenic
CCATTGCTCATAAGGTTATG+TGG	0.439545	2.2:+2214670	MS.gene36348:CDS
CTGGCTATGTTGCAGCTACA+TGG	0.442108	2.2:+2214715	MS.gene36348:CDS
CTCTCACTTACATGAGGCTC+AGG	0.446135	2.2:-2214259	None:intergenic
AGATAAGGAAAGTGAAGATT+CGG	0.451816	2.2:+2214914	MS.gene36348:CDS
AAAAGAAAGGGAAGCGGAGA+CGG	0.453963	2.2:+2214879	MS.gene36348:CDS
CAAGTATGGTTGCATTGAAA+TGG	0.461985	2.2:+2212395	MS.gene36348:CDS
TAAAGGTATCATCTGTTCTC+TGG	0.462477	2.2:-2212350	None:intergenic
ATATGGATGTTATGACGCTT+TGG	0.463213	2.2:+2213480	MS.gene36348:CDS
CAAAAGTGGATATTAATACT+AGG	0.468606	2.2:+2213960	MS.gene36348:CDS
GTTGAATCTATCTGAGATTG+TGG	0.470881	2.2:+2212632	MS.gene36348:CDS
TTTCACTATGCTTGAAAATG+TGG	0.474145	2.2:-2212307	None:intergenic
GTGCACAGACAAGATAGATA+TGG	0.475273	2.2:+2213538	MS.gene36348:CDS
TTGAATCTATCTGAGATTGT+GGG	0.482370	2.2:+2212633	MS.gene36348:CDS
TGCTTGTTGAACACTGGCTT+AGG	0.483216	2.2:+2214838	MS.gene36348:CDS
AGTTTCAGGCGGTCGCCATA+AGG	0.484353	2.2:+2213582	MS.gene36348:CDS
TACTTGGAAGCAAGGTGTAA+TGG	0.487753	2.2:-2212378	None:intergenic
TTCAAGTGATCGACGAGAAA+GGG	0.488121	2.2:+2213227	MS.gene36348:CDS
ACCATTGATATACCTCTCAT+TGG	0.496126	2.2:-2214519	None:intergenic
AACAATGACTACATATAAAG+AGG	0.497577	2.2:-2214598	None:intergenic
TTAAGCTTGCAAGCAGCTGT+TGG	0.499976	2.2:-2212543	None:intergenic
AGAAAGTCTAGGTGTTGGTG+AGG	0.500055	2.2:-2214131	None:intergenic
AACACTGGCTTAGGAAATCC+AGG	0.505473	2.2:+2214847	MS.gene36348:CDS
TCGATCACTTGAATAAGATC+TGG	0.516330	2.2:-2213217	None:intergenic
ATTCAAGTGATCGACGAGAA+AGG	0.519519	2.2:+2213226	MS.gene36348:CDS
CGGAACTGCTGAATCAGATA+AGG	0.521722	2.2:+2214899	MS.gene36348:CDS
CTTGGTCAACAGGACAAAAG+TGG	0.523539	2.2:+2213946	MS.gene36348:CDS
GTGCTACTAGCACTTGGTGG+TGG	0.525873	2.2:+2214780	MS.gene36348:CDS
AAGGTGTAATGGAGTGCTAA+AGG	0.527867	2.2:-2212367	None:intergenic
AGAAAGAAAAGAAAGGGAAG+CGG	0.530971	2.2:+2214873	MS.gene36348:CDS
GTGTGATGCTTGTTGAACAC+TGG	0.535308	2.2:+2214832	MS.gene36348:CDS
TTCTTGGTGAGAGTTGCCTA+TGG	0.538930	2.2:+2213505	MS.gene36348:CDS
TAGCCATGGTCATCTTGACT+TGG	0.541944	2.2:+2212599	MS.gene36348:CDS
AGGTGGAGAAAGAAAAGAAA+GGG	0.545486	2.2:+2214867	MS.gene36348:CDS
ATGGTCAATAGTATTGTTTG+TGG	0.546036	2.2:-2214651	None:intergenic
CCTTCTTGGTACACACCACC+AGG	0.547653	2.2:-2214495	None:intergenic
CACATAATCAAGAGATGCAA+TGG	0.548642	2.2:+2214748	MS.gene36348:CDS
ATTGTGGGGCAAGAAGTAGC+AGG	0.549345	2.2:+2212648	MS.gene36348:CDS
TGCATCTTGCAGTGATTAAG+GGG	0.555428	2.2:+2213362	MS.gene36348:CDS
TTAGAGAGCTGTTGAACAAG+TGG	0.556335	2.2:+2213194	MS.gene36348:CDS
TCCATGTTGCTGCAGTAAGA+GGG	0.561770	2.2:+2212688	MS.gene36348:CDS
TAAGGTTATGTGGGTTGCTG+TGG	0.566841	2.2:+2214680	MS.gene36348:CDS
TGCAACCATACTTGGAAGCA+AGG	0.569404	2.2:-2212386	None:intergenic
GAAAGTCTAGGTGTTGGTGA+GGG	0.571191	2.2:-2214130	None:intergenic
GCTGCTGGTATTAATCCACC+TGG	0.571700	2.2:+2214477	MS.gene36348:CDS
GTGCACTAGATTTGTACCAT+AGG	0.574161	2.2:-2213521	None:intergenic
TTAATCACTGCAAGATGCAA+TGG	0.576969	2.2:-2213358	None:intergenic
GTTGTGCTACTAGCACTTGG+TGG	0.579135	2.2:+2214777	MS.gene36348:CDS
CAGGTGGAGAAAGAAAAGAA+AGG	0.580805	2.2:+2214866	MS.gene36348:CDS
TGTTAGATTATGCCCTGACA+TGG	0.583786	2.2:+2212428	MS.gene36348:CDS
CAAGCCACATTTATCAGAGA+TGG	0.584991	2.2:+2214049	MS.gene36348:CDS
ACCAATGAGAGGTATATCAA+TGG	0.585047	2.2:+2214518	MS.gene36348:CDS
ACCGCCATCTCTGATAAATG+TGG	0.591360	2.2:-2214053	None:intergenic
GCTTTACAATACAACAACAA+TGG	0.591686	2.2:+2213328	MS.gene36348:CDS
TTTCAAAGCTCTTATCTACA+GGG	0.595521	2.2:+2214939	MS.gene36348:CDS
TCTGTTGTGCTACTAGCACT+TGG	0.599953	2.2:+2214774	MS.gene36348:CDS
TACTACCACAAAATAGTCGA+GGG	0.601617	2.2:-2213395	None:intergenic
CATTGCTCATAAGGTTATGT+GGG	0.602691	2.2:+2214671	MS.gene36348:CDS
CTTCGCGAGGAAGAAAGTCT+AGG	0.603728	2.2:-2214142	None:intergenic
GAGGAAGAAAGTCTAGGTGT+TGG	0.606764	2.2:-2214136	None:intergenic
GCCCTCTTACTGCAGCAACA+TGG	0.606854	2.2:-2212689	None:intergenic
TTACATCTCGCAGTTTCAGG+CGG	0.606870	2.2:+2213571	MS.gene36348:CDS
ATGAGAGGTATATCAATGGT+TGG	0.607766	2.2:+2214522	MS.gene36348:CDS
TCTCAGATAGATTCAACAAG+AGG	0.608966	2.2:-2212626	None:intergenic
TGGCTATGTTGCAGCTACAT+GGG	0.618021	2.2:+2214716	MS.gene36348:CDS
CCACATAACCTTATGAGCAA+TGG	0.619714	2.2:-2214670	None:intergenic
AGCATAGTGAAAGAAAGAGA+AGG	0.622329	2.2:+2212318	MS.gene36348:CDS
CTCGCGAAGGTATACACCGA+AGG	0.626060	2.2:+2214156	MS.gene36348:CDS
CCTGGTGGTGTGTACCAAGA+AGG	0.628111	2.2:+2214495	MS.gene36348:CDS
CTAGACTTTCTTCCTCGCGA+AGG	0.631029	2.2:+2214143	MS.gene36348:CDS
TTACTACCACAAAATAGTCG+AGG	0.634588	2.2:-2213396	None:intergenic
TTTATAACAAGCATGATGCA+AGG	0.645651	2.2:-2213257	None:intergenic
TACCAAGAAGGACCAATGAG+AGG	0.646349	2.2:+2214507	MS.gene36348:CDS
TTGTGGGGCAAGAAGTAGCA+GGG	0.651054	2.2:+2212649	MS.gene36348:CDS
TCAAATCTCTCACTTACATG+AGG	0.653146	2.2:-2214265	None:intergenic
GCCACATTTATCAGAGATGG+CGG	0.655061	2.2:+2214052	MS.gene36348:CDS
CACGGAGACTCCAATTCATG+AGG	0.659217	2.2:+2212470	MS.gene36348:CDS
CTTCGGTGTATACCTTCGCG+AGG	0.660706	2.2:-2214155	None:intergenic
TTGTAGACAAGAGAATGTCA+AGG	0.662532	2.2:+2212494	MS.gene36348:CDS
TCTCAGCAGAAACCATGTCA+GGG	0.665903	2.2:-2212440	None:intergenic
ACCAGCAGCAAAAGTTACAG+TGG	0.670740	2.2:-2214463	None:intergenic
GCTGCAGTAAGAGGGCACAC+AGG	0.671839	2.2:+2212696	MS.gene36348:CDS
TCTTGGTACACACCACCAGG+TGG	0.673788	2.2:-2214492	None:intergenic
TGAATCTATCTGAGATTGTG+GGG	0.685330	2.2:+2212634	MS.gene36348:CDS
GCTGGTATTAATCCACCTGG+TGG	0.710773	2.2:+2214480	MS.gene36348:CDS
TCACCAAGTCAAGATGACCA+TGG	0.728525	2.2:-2212602	None:intergenic
ACTGGCTTAGGAAATCCAGG+TGG	0.784007	2.2:+2214850	MS.gene36348:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTGACGTTTAATTAACAA+AGG	-	chr2.2:2212904-2212923	None:intergenic	20.0%
!!	TTCAAGAAGAAAATTCAATA+TGG	-	chr2.2:2213694-2213713	None:intergenic	20.0%
!	AACAATGACTACATATAAAG+AGG	-	chr2.2:2214601-2214620	None:intergenic	25.0%
!	AAGAAACACTATTGTATTAG+TGG	+	chr2.2:2214428-2214447	MS.gene36348:intron	25.0%
!	ATTTATTTGTCTGTAGTATC+TGG	+	chr2.2:2213842-2213861	MS.gene36348:intron	25.0%
!	CAAAAGTGGATATTAATACT+AGG	+	chr2.2:2213960-2213979	MS.gene36348:CDS	25.0%
!	CAATTCAATAGTTATGATCA+TGG	+	chr2.2:2213895-2213914	MS.gene36348:intron	25.0%
!	CATCATAACAAAGGAAAAAT+TGG	+	chr2.2:2214349-2214368	MS.gene36348:intron	25.0%
!	CCTTATGACATATTAATTCA+AGG	+	chr2.2:2212799-2212818	MS.gene36348:intron	25.0%
!	CCTTGAATTAATATGTCATA+AGG	-	chr2.2:2212802-2212821	None:intergenic	25.0%
!!!	AAAAAAGCACTTTTACAAGT+AGG	-	chr2.2:2212570-2212589	None:intergenic	25.0%
!!!	TTGTAAAAGTGCTTTTTTTG+TGG	+	chr2.2:2212572-2212591	MS.gene36348:CDS	25.0%
!!!	TTTTGTTTCACTAAATCAGT+TGG	-	chr2.2:2214325-2214344	None:intergenic	25.0%
	AAGGACTAATAATAAGGTGA+TGG	+	chr2.2:2213106-2213125	MS.gene36348:intron	30.0%
	AGATAAGGAAAGTGAAGATT+CGG	+	chr2.2:2214914-2214933	MS.gene36348:CDS	30.0%
	ATGCAAGTAAACAGTATATC+AGG	+	chr2.2:2213814-2213833	MS.gene36348:intron	30.0%
	ATGGTCAATAGTATTGTTTG+TGG	-	chr2.2:2214654-2214673	None:intergenic	30.0%
	ATTGAGCGAATTAGATAAAC+AGG	+	chr2.2:2212854-2212873	MS.gene36348:intron	30.0%
	CACATAAATTTCTCACCTTA+TGG	-	chr2.2:2213600-2213619	None:intergenic	30.0%
	GAACCTAAGGACTAATAATA+AGG	+	chr2.2:2213100-2213119	MS.gene36348:intron	30.0%
	GATGTAAGTTATCTCCTAAA+TGG	+	chr2.2:2213149-2213168	MS.gene36348:intron	30.0%
	GCTTTACAATACAACAACAA+TGG	+	chr2.2:2213328-2213347	MS.gene36348:CDS	30.0%
	GGTTCTATAATCTTTGTAGA+TGG	+	chr2.2:2213916-2213935	MS.gene36348:intron	30.0%
	GTAACAATTGATATACCTAG+AGG	+	chr2.2:2213058-2213077	MS.gene36348:intron	30.0%
	TCACCTTATTATTAGTCCTT+AGG	-	chr2.2:2213106-2213125	None:intergenic	30.0%
	TTCTGCTGAGAATAAAAACA+CGG	+	chr2.2:2212452-2212471	MS.gene36348:CDS	30.0%
	TTTATAACAAGCATGATGCA+AGG	-	chr2.2:2213260-2213279	None:intergenic	30.0%
	TTTCAAAGCTCTTATCTACA+GGG	+	chr2.2:2214939-2214958	MS.gene36348:CDS	30.0%
	TTTCACTATGCTTGAAAATG+TGG	-	chr2.2:2212310-2212329	None:intergenic	30.0%
!	GATTCAATGCTTTTGCTAAA+CGG	-	chr2.2:2214232-2214251	None:intergenic	30.0%
!	GTTTGTGGTTTTCTTCTAAA+TGG	-	chr2.2:2214639-2214658	None:intergenic	30.0%
!	TGCGTAGTAATATGGATTAT+AGG	-	chr2.2:2214299-2214318	None:intergenic	30.0%
!	TTGAATCTATCTGAGATTGT+GGG	+	chr2.2:2212633-2212652	MS.gene36348:CDS	30.0%
!	TTTTAACGCTATAGTCTATG+TGG	-	chr2.2:2213003-2213022	None:intergenic	30.0%
!	TTTTCAAAGCTCTTATCTAC+AGG	+	chr2.2:2214938-2214957	MS.gene36348:CDS	30.0%
!!	CAAAAGCATCATCATAACAA+AGG	+	chr2.2:2214340-2214359	MS.gene36348:intron	30.0%
!!	TTGCATCATGCTTGTTATAA+AGG	+	chr2.2:2213259-2213278	MS.gene36348:CDS	30.0%
!!!	AAGGACATTTTGAGATTGTA+TGG	+	chr2.2:2213278-2213297	MS.gene36348:CDS	30.0%
	ACCATTGATATACCTCTCAT+TGG	-	chr2.2:2214522-2214541	None:intergenic	35.0%
	ACTATTGACCATTGCTCATA+AGG	+	chr2.2:2214662-2214681	MS.gene36348:CDS	35.0%
	AGAAAGAAAAGAAAGGGAAG+CGG	+	chr2.2:2214873-2214892	MS.gene36348:CDS	35.0%
	AGCATAGTGAAAGAAAGAGA+AGG	+	chr2.2:2212318-2212337	MS.gene36348:CDS	35.0%
	AGGACACAATTTGCACAATT+TGG	+	chr2.2:2213078-2213097	MS.gene36348:intron	35.0%
	AGGTGGAGAAAGAAAAGAAA+GGG	+	chr2.2:2214867-2214886	MS.gene36348:CDS	35.0%
	ATATGGATGTTATGACGCTT+TGG	+	chr2.2:2213480-2213499	MS.gene36348:CDS	35.0%
	ATTGCATCTTGCAGTGATTA+AGG	+	chr2.2:2213360-2213379	MS.gene36348:CDS	35.0%
	CAAGTATGGTTGCATTGAAA+TGG	+	chr2.2:2212395-2212414	MS.gene36348:CDS	35.0%
	CAAGTGAAAAAGAAGAAGAC+TGG	-	chr2.2:2214090-2214109	None:intergenic	35.0%
	CACATAATCAAGAGATGCAA+TGG	+	chr2.2:2214748-2214767	MS.gene36348:CDS	35.0%
	CATTGCATCTCTTGATTATG+TGG	-	chr2.2:2214750-2214769	None:intergenic	35.0%
	CATTGCTCATAAGGTTATGT+GGG	+	chr2.2:2214671-2214690	MS.gene36348:CDS	35.0%
	CATTTCAATGCAACCATACT+TGG	-	chr2.2:2212397-2212416	None:intergenic	35.0%
	GAAATGTGAATCATACCAAG+AGG	+	chr2.2:2214371-2214390	MS.gene36348:intron	35.0%
	GCATAGACAATCAACCATTT+AGG	-	chr2.2:2213166-2213185	None:intergenic	35.0%
	GTAAGTGAGAGATTTGAGAA+TGG	+	chr2.2:2214271-2214290	MS.gene36348:intron	35.0%
	TACTACCACAAAATAGTCGA+GGG	-	chr2.2:2213398-2213417	None:intergenic	35.0%
	TCAAATCTCTCACTTACATG+AGG	-	chr2.2:2214268-2214287	None:intergenic	35.0%
	TCGATCACTTGAATAAGATC+TGG	-	chr2.2:2213220-2213239	None:intergenic	35.0%
	TCTCAGATAGATTCAACAAG+AGG	-	chr2.2:2212629-2212648	None:intergenic	35.0%
	TTAATCACTGCAAGATGCAA+TGG	-	chr2.2:2213361-2213380	None:intergenic	35.0%
	TTACTACCACAAAATAGTCG+AGG	-	chr2.2:2213399-2213418	None:intergenic	35.0%
	TTGCATCTTGCAGTGATTAA+GGG	+	chr2.2:2213361-2213380	MS.gene36348:CDS	35.0%
	TTGTAGACAAGAGAATGTCA+AGG	+	chr2.2:2212494-2212513	MS.gene36348:CDS	35.0%
	TTTGTAGATGGCAGATTTCT+TGG	+	chr2.2:2213928-2213947	MS.gene36348:intron	35.0%
!	ACCAATGAGAGGTATATCAA+TGG	+	chr2.2:2214518-2214537	MS.gene36348:CDS	35.0%
!	ATGAGAGGTATATCAATGGT+TGG	+	chr2.2:2214522-2214541	MS.gene36348:CDS	35.0%
!	CAAGGCTGAATTGTTCTTTT+TGG	+	chr2.2:2212817-2212836	MS.gene36348:intron	35.0%
!	CTCATGATAGTTTTTCCTCT+TGG	-	chr2.2:2214389-2214408	None:intergenic	35.0%
!	GTTGAATCTATCTGAGATTG+TGG	+	chr2.2:2212632-2212651	MS.gene36348:CDS	35.0%
!	TGAATCTATCTGAGATTGTG+GGG	+	chr2.2:2212634-2212653	MS.gene36348:CDS	35.0%
!	TGGTGAAACATCAGCTTTTA+AGG	+	chr2.2:2214542-2214561	MS.gene36348:CDS	35.0%
!	TTTGCACAATTTGGAACCTA+AGG	+	chr2.2:2213087-2213106	MS.gene36348:intron	35.0%
!!	AATGGAAGTGCTTAGTGAAA+TGG	+	chr2.2:2214180-2214199	MS.gene36348:CDS	35.0%
!!	GCTGAATTGTTCTTTTTGGT+AGG	+	chr2.2:2212821-2212840	MS.gene36348:intron	35.0%
!!	TAAAGGTATCATCTGTTCTC+TGG	-	chr2.2:2212353-2212372	None:intergenic	35.0%
!!!	TTATGACGCTTTGGTTTTCT+TGG	+	chr2.2:2213489-2213508	MS.gene36348:CDS	35.0%
	AAGGTATACACCGAAGGAAA+TGG	+	chr2.2:2214162-2214181	MS.gene36348:CDS	40.0%
	ACTGTCTCTTCTTCTCTTGT+GGG	-	chr2.2:2213442-2213461	None:intergenic	40.0%
	ACTTAGACAACTTAGGCTCT+AGG	-	chr2.2:2212935-2212954	None:intergenic	40.0%
	AGAGCCTAAGTTGTCTAAGT+TGG	+	chr2.2:2212935-2212954	MS.gene36348:intron	40.0%
	ATTCAAGTGATCGACGAGAA+AGG	+	chr2.2:2213226-2213245	MS.gene36348:CDS	40.0%
	CAAGCCACATTTATCAGAGA+TGG	+	chr2.2:2214049-2214068	MS.gene36348:CDS	40.0%
	CAGGTGGAGAAAGAAAAGAA+AGG	+	chr2.2:2214866-2214885	MS.gene36348:CDS	40.0%
	CCACATAACCTTATGAGCAA+TGG	-	chr2.2:2214673-2214692	None:intergenic	40.0%
	CCATTGCTCATAAGGTTATG+TGG	+	chr2.2:2214670-2214689	MS.gene36348:CDS	40.0%
	CTAAGCACTTCCATTTCCTT+CGG	-	chr2.2:2214175-2214194	None:intergenic	40.0%
	CTTGCTTAGCCAGAATAAGT+TGG	-	chr2.2:2212962-2212981	None:intergenic	40.0%
	GAGCCTAAGTTGTCTAAGTT+GGG	+	chr2.2:2212936-2212955	MS.gene36348:intron	40.0%
	GTGCACAGACAAGATAGATA+TGG	+	chr2.2:2213538-2213557	MS.gene36348:CDS	40.0%
	GTGCACTAGATTTGTACCAT+AGG	-	chr2.2:2213524-2213543	None:intergenic	40.0%
	TACTGTCTCTTCTTCTCTTG+TGG	-	chr2.2:2213443-2213462	None:intergenic	40.0%
	TACTTGGAAGCAAGGTGTAA+TGG	-	chr2.2:2212381-2212400	None:intergenic	40.0%
	TGCATCTTGCAGTGATTAAG+GGG	+	chr2.2:2213362-2213381	MS.gene36348:CDS	40.0%
	TGTCTACAAGCCTCATGAAT+TGG	-	chr2.2:2212483-2212502	None:intergenic	40.0%
	TGTTAGATTATGCCCTGACA+TGG	+	chr2.2:2212428-2212447	MS.gene36348:CDS	40.0%
	TTACACCTTGCTTCCAAGTA+TGG	+	chr2.2:2212381-2212400	MS.gene36348:CDS	40.0%
	TTAGAGAGCTGTTGAACAAG+TGG	+	chr2.2:2213194-2213213	MS.gene36348:CDS	40.0%
	TTCAAGTGATCGACGAGAAA+GGG	+	chr2.2:2213227-2213246	MS.gene36348:CDS	40.0%
	TTGTGCAAATTGTGTCCTCT+AGG	-	chr2.2:2213076-2213095	None:intergenic	40.0%
	TTTAATCTGTGCAACGTTCG+AGG	+	chr2.2:2213645-2213664	MS.gene36348:intron	40.0%
!	AAGGTGTAATGGAGTGCTAA+AGG	-	chr2.2:2212370-2212389	None:intergenic	40.0%
!	CTTCATCTTGCTGTGAGATA+TGG	+	chr2.2:2213463-2213482	MS.gene36348:CDS	40.0%
!	CTTTCTTTTCTTTCTCCACC+TGG	-	chr2.2:2214868-2214887	None:intergenic	40.0%
!	TAAGTTGGGCCAACTTATTC+TGG	+	chr2.2:2212950-2212969	MS.gene36348:intron	40.0%
!	TCAATGCTTTTGCTAAACGG+TGG	-	chr2.2:2214229-2214248	None:intergenic	40.0%
!!	AAGGTTTTGATGTTGCTGCT+GGG	+	chr2.2:2212513-2212532	MS.gene36348:CDS	40.0%
!!	GTTGGTGATGCGTAGTAATA+TGG	-	chr2.2:2214307-2214326	None:intergenic	40.0%
!!!	TTTTTTGTGGCTTGTAGCCA+TGG	+	chr2.2:2212585-2212604	MS.gene36348:CDS	40.0%
	AAAAGAAAGGGAAGCGGAGA+CGG	+	chr2.2:2214879-2214898	MS.gene36348:CDS	45.0%
	ACCAGCAGCAAAAGTTACAG+TGG	-	chr2.2:2214466-2214485	None:intergenic	45.0%
	ACCGCCATCTCTGATAAATG+TGG	-	chr2.2:2214056-2214075	None:intergenic	45.0%
	CGGAACTGCTGAATCAGATA+AGG	+	chr2.2:2214899-2214918	MS.gene36348:CDS	45.0%
	CTTGGTCAACAGGACAAAAG+TGG	+	chr2.2:2213946-2213965	MS.gene36348:CDS	45.0%
	GAGGAAGAAAGTCTAGGTGT+TGG	-	chr2.2:2214139-2214158	None:intergenic	45.0%
	GCCACATTTATCAGAGATGG+CGG	+	chr2.2:2214052-2214071	MS.gene36348:CDS	45.0%
	GCTGTGGCATTTATGTCAAC+TGG	+	chr2.2:2214696-2214715	MS.gene36348:CDS	45.0%
	GTCTTACATCTCGCAGTTTC+AGG	+	chr2.2:2213568-2213587	MS.gene36348:CDS	45.0%
	TACCAAGAAGGACCAATGAG+AGG	+	chr2.2:2214507-2214526	MS.gene36348:CDS	45.0%
	TACCTCTCATTGGTCCTTCT+TGG	-	chr2.2:2214512-2214531	None:intergenic	45.0%
	TAGCCATGGTCATCTTGACT+TGG	+	chr2.2:2212599-2212618	MS.gene36348:CDS	45.0%
	TCACCAAGTCAAGATGACCA+TGG	-	chr2.2:2212605-2212624	None:intergenic	45.0%
	TCCATGTTGCTGCAGTAAGA+GGG	+	chr2.2:2212688-2212707	MS.gene36348:CDS	45.0%
	TCTCAGCAGAAACCATGTCA+GGG	-	chr2.2:2212443-2212462	None:intergenic	45.0%
	TGCAACCATACTTGGAAGCA+AGG	-	chr2.2:2212389-2212408	None:intergenic	45.0%
	TGGCAGATTTCTTGGTCAAC+AGG	+	chr2.2:2213936-2213955	MS.gene36348:CDS	45.0%
	TGGCCCAACTTAGACAACTT+AGG	-	chr2.2:2212942-2212961	None:intergenic	45.0%
	TGGCTATGTTGCAGCTACAT+GGG	+	chr2.2:2214716-2214735	MS.gene36348:CDS	45.0%
	TTAAGCTTGCAAGCAGCTGT+TGG	-	chr2.2:2212546-2212565	None:intergenic	45.0%
	TTACATCTCGCAGTTTCAGG+CGG	+	chr2.2:2213571-2213590	MS.gene36348:CDS	45.0%
	TTCCATGTTGCTGCAGTAAG+AGG	+	chr2.2:2212687-2212706	MS.gene36348:CDS	45.0%
	TTCTCAGCAGAAACCATGTC+AGG	-	chr2.2:2212444-2212463	None:intergenic	45.0%
	TTCTTGGTGAGAGTTGCCTA+TGG	+	chr2.2:2213505-2213524	MS.gene36348:CDS	45.0%
!	ATGCTTTTGCTAAACGGTGG+AGG	-	chr2.2:2214226-2214245	None:intergenic	45.0%
!	CTCAACCCTCGACTATTTTG+TGG	+	chr2.2:2213390-2213409	MS.gene36348:CDS	45.0%
!	GTGTGATGCTTGTTGAACAC+TGG	+	chr2.2:2214832-2214851	MS.gene36348:CDS	45.0%
!	TAAGGTTATGTGGGTTGCTG+TGG	+	chr2.2:2214680-2214699	MS.gene36348:CDS	45.0%
!	TCTGTTGTGCTACTAGCACT+TGG	+	chr2.2:2214774-2214793	MS.gene36348:CDS	45.0%
!	TGCTTTTGCTAAACGGTGGA+GGG	-	chr2.2:2214225-2214244	None:intergenic	45.0%
!!	AACACTGGCTTAGGAAATCC+AGG	+	chr2.2:2214847-2214866	MS.gene36348:CDS	45.0%
!!	AGAAAGTCTAGGTGTTGGTG+AGG	-	chr2.2:2214134-2214153	None:intergenic	45.0%
!!	CAAGGTTTTGATGTTGCTGC+TGG	+	chr2.2:2212512-2212531	MS.gene36348:CDS	45.0%
!!	GAAAGTCTAGGTGTTGGTGA+GGG	-	chr2.2:2214133-2214152	None:intergenic	45.0%
!!	TGCTTGTTGAACACTGGCTT+AGG	+	chr2.2:2214838-2214857	MS.gene36348:CDS	45.0%
	ATTGTGGGGCAAGAAGTAGC+AGG	+	chr2.2:2212648-2212667	MS.gene36348:CDS	50.0%
	CACGGAGACTCCAATTCATG+AGG	+	chr2.2:2212470-2212489	MS.gene36348:CDS	50.0%
	CTAGACTTTCTTCCTCGCGA+AGG	+	chr2.2:2214143-2214162	MS.gene36348:CDS	50.0%
	CTCTCACTTACATGAGGCTC+AGG	-	chr2.2:2214262-2214281	None:intergenic	50.0%
	CTGGCTATGTTGCAGCTACA+TGG	+	chr2.2:2214715-2214734	MS.gene36348:CDS	50.0%
	CTTCGCGAGGAAGAAAGTCT+AGG	-	chr2.2:2214145-2214164	None:intergenic	50.0%
	GCTGCTGGTATTAATCCACC+TGG	+	chr2.2:2214477-2214496	MS.gene36348:CDS	50.0%
	GCTGGTATTAATCCACCTGG+TGG	+	chr2.2:2214480-2214499	MS.gene36348:CDS	50.0%
	TTGTGGGGCAAGAAGTAGCA+GGG	+	chr2.2:2212649-2212668	MS.gene36348:CDS	50.0%
!	ACTGGCTTAGGAAATCCAGG+TGG	+	chr2.2:2214850-2214869	MS.gene36348:CDS	50.0%
!	GCCACTGTAACTTTTGCTGC+TGG	+	chr2.2:2214462-2214481	MS.gene36348:CDS	50.0%
!	GTTGTGCTACTAGCACTTGG+TGG	+	chr2.2:2214777-2214796	MS.gene36348:CDS	50.0%
	AGTTTCAGGCGGTCGCCATA+AGG	+	chr2.2:2213582-2213601	MS.gene36348:CDS	55.0%
	CCTTCTTGGTACACACCACC+AGG	-	chr2.2:2214498-2214517	None:intergenic	55.0%
	GCCCTCTTACTGCAGCAACA+TGG	-	chr2.2:2212692-2212711	None:intergenic	55.0%
	TCTTGGTACACACCACCAGG+TGG	-	chr2.2:2214495-2214514	None:intergenic	55.0%
!	CAGTAAGAGGGCACACAGGT+GGG	+	chr2.2:2212700-2212719	MS.gene36348:intron	55.0%
!	CCTGGTGGTGTGTACCAAGA+AGG	+	chr2.2:2214495-2214514	MS.gene36348:CDS	55.0%
!	CTCGCGAAGGTATACACCGA+AGG	+	chr2.2:2214156-2214175	MS.gene36348:CDS	55.0%
!	CTTCGGTGTATACCTTCGCG+AGG	-	chr2.2:2214158-2214177	None:intergenic	55.0%
!	GTGCTACTAGCACTTGGTGG+TGG	+	chr2.2:2214780-2214799	MS.gene36348:CDS	55.0%
!!	GCACTTGGTGGTGGCTTTCT+AGG	+	chr2.2:2214789-2214808	MS.gene36348:CDS	55.0%
!	GCAGTAAGAGGGCACACAGG+TGG	+	chr2.2:2212699-2212718	MS.gene36348:intron	60.0%
!	GCTGCAGTAAGAGGGCACAC+AGG	+	chr2.2:2212696-2212715	MS.gene36348:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	2212264	2214977	2212264	ID=MS.gene36348
chr2.2	mRNA	2212264	2214977	2212264	ID=MS.gene36348.t1;Parent=MS.gene36348
chr2.2	exon	2212264	2212717	2212264	ID=MS.gene36348.t1.exon1;Parent=MS.gene36348.t1
chr2.2	CDS	2212264	2212717	2212264	ID=cds.MS.gene36348.t1;Parent=MS.gene36348.t1
chr2.2	exon	2213188	2213603	2213188	ID=MS.gene36348.t1.exon2;Parent=MS.gene36348.t1
chr2.2	CDS	2213188	2213603	2213188	ID=cds.MS.gene36348.t1;Parent=MS.gene36348.t1
chr2.2	exon	2213935	2214274	2213935	ID=MS.gene36348.t1.exon3;Parent=MS.gene36348.t1
chr2.2	CDS	2213935	2214274	2213935	ID=cds.MS.gene36348.t1;Parent=MS.gene36348.t1
chr2.2	exon	2214448	2214977	2214448	ID=MS.gene36348.t1.exon4;Parent=MS.gene36348.t1
chr2.2	CDS	2214448	2214977	2214448	ID=cds.MS.gene36348.t1;Parent=MS.gene36348.t1

Gene Sequence

>MS.gene36348

ATGGATCAAGAGTTTTTCGATGCAATTAAGAAAAATGACATGACCACATTTTCAAGCATAGTGAAAGAAAGAGAAGGAATTCTCAACCAGAGAACAGATGATACCTTTAGCACTCCATTACACCTTGCTTCCAAGTATGGTTGCATTGAAATGGTGTCTGAGATTGTTAGATTATGCCCTGACATGGTTTCTGCTGAGAATAAAAACACGGAGACTCCAATTCATGAGGCTTGTAGACAAGAGAATGTCAAGGTTTTGATGTTGCTGCTGGGAGTCAATCCAACAGCTGCTTGCAAGCTTAATCCTACTTGTAAAAGTGCTTTTTTTGTGGCTTGTAGCCATGGTCATCTTGACTTGGTGAACCTCTTGTTGAATCTATCTGAGATTGTGGGGCAAGAAGTAGCAGGGTTTGATCAAACTTGTTTCCATGTTGCTGCAGTAAGAGGGCACACAGGTGGGAAGAAAGAGTTGTATTCACTATGTTTGCATGCTTTTTGAACTATTATTTTTAATGAGTATTGAACTTATATTTTAGCCTTATGACATATTAATTCAAGGCTGAATTGTTCTTTTTGGTAGGCTGTGTTTCAATTGAGCGAATTAGATAAACAGGTATTCTAAATTTTACAATTAGATTCCTTTGTTAATTAAACGTCAAATTTTTAACGCCTAGAGCCTAAGTTGTCTAAGTTGGGCCAACTTATTCTGGCTAAGCAAGCTTCAAGACTCACGTTGACCACATAGACTATAGCGTTAAAAAATAGTAGACGATTAATAGATAGATGAATTTGATTGTAACAATTGATATACCTAGAGGACACAATTTGCACAATTTGGAACCTAAGGACTAATAATAAGGTGATGGATTGAAAATCTTATATAGCTGATGTAAGTTATCTCCTAAATGGTTGATTGTCTATGCAGATGTTGTTAGAGAGCTGTTGAACAAGTGGCCAGATCTTATTCAAGTGATCGACGAGAAAGGGAATACAGCCTTGCATCATGCTTGTTATAAAGGACATTTTGAGATTGTATGGATCATACTAAGTCGCGATTCAAATCTTGCTTTACAATACAACAACAATGGTTATACACCATTGCATCTTGCAGTGATTAAGGGGAAAGTCTCAACCCTCGACTATTTTGTGGTAGTAAGTACTGCATACTTTCACTATCCCACAAGAGAAGAAGAGACAGTACTTCATCTTGCTGTGAGATATGGATGTTATGACGCTTTGGTTTTCTTGGTGAGAGTTGCCTATGGTACAAATCTAGTGCACAGACAAGATAGATATGGCAACACTGTCTTACATCTCGCAGTTTCAGGCGGTCGCCATAAGGTGAGAAATTTATGTGAGCTCTGAACATATTTAACTAGTGTTTTAATCTGTGCAACGTTCGAGGAGATAATTTTAGTTTAATTATATCCATATTGAATTTTCTTCTTGAAAAGTAGTAACTGTTGTAATTTGAGAGCATTGAAATGATTTAAATACTGAAAAGTAACATTTAAGATTTTTCTAATGTTTGTTGTTTTGAAATCATGACAAATGCAAGTAAACAGTATATCAGGCATCAATTTATTTGTCTGTAGTATCTGGCTTTAGTACTTAAGCTCTTTTAATATTATTCAATTCAATAGTTATGATCATGGTTCTATAATCTTTGTAGATGGCAGATTTCTTGGTCAACAGGACAAAAGTGGATATTAATACTAGGAATAATGAAGAGCTCACAGCACTTGACATTCTTGATCAGAGTGACAGTGCAGAAAATAGACAACTACAAGCCACATTTATCAGAGATGGCGGTAAAAGAAGCTTCCAGTCTTCTTCTTTTTCACTTGAACTAGACAACACTAATTCTCCCTCACCAACACCTAGACTTTCTTCCTCGCGAAGGTATACACCGAAGGAAATGGAAGTGCTTAGTGAAATGGTTTCTTATGATTGTACAAGCCCTCCACCGTTTAGCAAAAGCATTGAATCTACATCACCTGAGCCTCATGTAAGTGAGAGATTTGAGAATGGAACCTATAATCCATATTACTACGCATCACCAACTGATTTAGTGAAACAAAAGCATCATCATAACAAAGGAAAAATTGGAAATGTGAATCATACCAAGAGGAAAAACTATCATGAGATGCACAAAGACTAAATGCAAGAAACACTATTGTATTAGTGGCTGTTTTGATTGCCACTGTAACTTTTGCTGCTGGTATTAATCCACCTGGTGGTGTGTACCAAGAAGGACCAATGAGAGGTATATCAATGGTTGGTGAAACATCAGCTTTTAAGGTATTTGCAATAAGCAATATCATAGCGCTCTTCACCTCTTTATATGTAGTCATTGTTCTTGTTAGTATCATTCCATTTAGAAGAAAACCACAAACAATACTATTGACCATTGCTCATAAGGTTATGTGGGTTGCTGTGGCATTTATGTCAACTGGCTATGTTGCAGCTACATGGGTGATTTTGCCACATAATCAAGAGATGCAATGGCTATCTGTTGTGCTACTAGCACTTGGTGGTGGCTTTCTAGGCACGATATTCATTAGTCTTAGTGTGATGCTTGTTGAACACTGGCTTAGGAAATCCAGGTGGAGAAAGAAAAGAAAGGGAAGCGGAGACGGAACTGCTGAATCAGATAAGGAAAGTGAAGATTCGGATTTTCAAAGCTCTTATCTACAGGGTTATCATTCATATTGA

Protein sequence

>MS.gene36348.t1

MDQEFFDAIKKNDMTTFSSIVKEREGILNQRTDDTFSTPLHLASKYGCIEMVSEIVRLCPDMVSAENKNTETPIHEACRQENVKVLMLLLGVNPTAACKLNPTCKSAFFVACSHGHLDLVNLLLNLSEIVGQEVAGFDQTCFHVAAVRGHTDVVRELLNKWPDLIQVIDEKGNTALHHACYKGHFEIVWIILSRDSNLALQYNNNGYTPLHLAVIKGKVSTLDYFVVVSTAYFHYPTREEETVLHLAVRYGCYDALVFLVRVAYGTNLVHRQDRYGNTVLHLAVSGGRHKMADFLVNRTKVDINTRNNEELTALDILDQSDSAENRQLQATFIRDGGKRSFQSSSFSLELDNTNSPSPTPRLSSSRRYTPKEMEVLSEMVSYDCTSPPPFSKSIESTSPEPHVMAVLIATVTFAAGINPPGGVYQEGPMRGISMVGETSAFKVFAISNIIALFTSLYVVIVLVSIIPFRRKPQTILLTIAHKVMWVAVAFMSTGYVAATWVILPHNQEMQWLSVVLLALGGGFLGTIFISLSVMLVEHWLRKSRWRKKRKGSGDGTAESDKESEDSDFQSSYLQGYHSY