AlfalfaGEDB | MS.gene85146

M. sativa cultivar XinJiangDaYe / MS.gene85146

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00023.33 PF12796.10 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85146.t1	XP_003597696.1	88.1	640	74	2	1	640	1	638	2.10E-185	659.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85146.t1	A2CIR5	33.5	158	95	3	435	588	387	538	1.8e-15	85.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85146.t1	G7IUM3	88.1	640	74	2	1	640	1	638	1.5e-185	659.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene98456	MS.gene85146	PPI
MS.gene85146	MS.gene02894	PPI
MS.gene85146	MS.gene40013	PPI
MS.gene85146	MS.gene01601	PPI
MS.gene029949	MS.gene85146	PPI
MS.gene85146	MS.gene69639	PPI
MS.gene69642	MS.gene85146	PPI
MS.gene85146	MS.gene93912	PPI
MS.gene85146	MS.gene012743	PPI
MS.gene85146	MS.gene003142	PPI
MS.gene85146	MS.gene76567	PPI
MS.gene85146	MS.gene98475	PPI
MS.gene048265	MS.gene85146	PPI
MS.gene85146	MS.gene43431	PPI
MS.gene018224	MS.gene85146	PPI
MS.gene01949	MS.gene85146	PPI
MS.gene85146	MS.gene36385	PPI
MS.gene85146	MS.gene067580	PPI
MS.gene000078	MS.gene85146	PPI
MS.gene000644	MS.gene85146	PPI
MS.gene85179	MS.gene85146	PPI
MS.gene85146	MS.gene93910	PPI
MS.gene85146	MS.gene72664	PPI
MS.gene011447	MS.gene85146	PPI
MS.gene85146	MS.gene000639	PPI
MS.gene000075	MS.gene85146	PPI
MS.gene65337	MS.gene85146	PPI
MS.gene85146	MS.gene95095	PPI
MS.gene94380	MS.gene85146	PPI
MS.gene85146	MS.gene046976	PPI
MS.gene82979	MS.gene85146	PPI
MS.gene69111	MS.gene85146	PPI
MS.gene98458	MS.gene85146	PPI
MS.gene79563	MS.gene85146	PPI
MS.gene85146	MS.gene86533	PPI
MS.gene027972	MS.gene85146	PPI
MS.gene03114	MS.gene85146	PPI
MS.gene64896	MS.gene85146	PPI
MS.gene008759	MS.gene85146	PPI
MS.gene85146	MS.gene86539	PPI
MS.gene85146	MS.gene000628	PPI
MS.gene85146	MS.gene62818	PPI
MS.gene56444	MS.gene85146	PPI
MS.gene85146	MS.gene070627	PPI
MS.gene046852	MS.gene85146	PPI
MS.gene85146	MS.gene027991	PPI
MS.gene85146	MS.gene061578	PPI
MS.gene85146	MS.gene98466	PPI
MS.gene006878	MS.gene85146	PPI
MS.gene033400	MS.gene85146	PPI
MS.gene85146	MS.gene37152	PPI
MS.gene027995	MS.gene85146	PPI
MS.gene85146	MS.gene002615	PPI
MS.gene98484	MS.gene85146	PPI
MS.gene004294	MS.gene85146	PPI
MS.gene042083	MS.gene85146	PPI
MS.gene000633	MS.gene85146	PPI
MS.gene85146	MS.gene32079	PPI
MS.gene47485	MS.gene85146	PPI
MS.gene85146	MS.gene79561	PPI
MS.gene85146	MS.gene045052	PPI
MS.gene000646	MS.gene85146	PPI
MS.gene027986	MS.gene85146	PPI
MS.gene85146	MS.gene98479	PPI
MS.gene35272	MS.gene85146	PPI
MS.gene85146	MS.gene070630	PPI
MS.gene85146	MS.gene000079	PPI
MS.gene85146	MS.gene05028	PPI
MS.gene69643	MS.gene85146	PPI
MS.gene004696	MS.gene85146	PPI
MS.gene79559	MS.gene85146	PPI
MS.gene82413	MS.gene85146	PPI
MS.gene85146	MS.gene043157	PPI
MS.gene85146	MS.gene000081	PPI
MS.gene008350	MS.gene85146	PPI
MS.gene85146	MS.gene049681	PPI
MS.gene85146	MS.gene62721	PPI
MS.gene85146	MS.gene046977	PPI
MS.gene85146	MS.gene48617	PPI
MS.gene85146	MS.gene39617	PPI
MS.gene69614	MS.gene85146	PPI
MS.gene046978	MS.gene85146	PPI
MS.gene93914	MS.gene85146	PPI
MS.gene85146	MS.gene036251	PPI
MS.gene69634	MS.gene85146	PPI
MS.gene027978	MS.gene85146	PPI
MS.gene85146	MS.gene26538	PPI
MS.gene85146	MS.gene98470	PPI
MS.gene021743	MS.gene85146	PPI
MS.gene000642	MS.gene85146	PPI
MS.gene000074	MS.gene85146	PPI
MS.gene85146	MS.gene37227	PPI
MS.gene85146	MS.gene94378	PPI
MS.gene85146	MS.gene069900	PPI
MS.gene72910	MS.gene85146	PPI
MS.gene85146	MS.gene057689	PPI
MS.gene85146	MS.gene014559	PPI
MS.gene78960	MS.gene85146	PPI
MS.gene045049	MS.gene85146	PPI
MS.gene40201	MS.gene85146	PPI
MS.gene42371	MS.gene85146	PPI
MS.gene85146	MS.gene82412	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85146.t1	MTR_2g101310	92.656	640	45	2	1	640	1	638	0.0	1190
MS.gene85146.t1	MTR_2g101320	84.219	640	100	1	1	640	1	639	0.0	1030
MS.gene85146.t1	MTR_2g101310	91.071	504	43	2	1	504	1	502	0.0	940
MS.gene85146.t1	MTR_1g115575	26.516	577	333	13	38	611	75	563	1.65e-46	174
MS.gene85146.t1	MTR_1g115575	23.790	248	188	1	1	247	96	343	5.26e-21	97.8
MS.gene85146.t1	MTR_7g100430	25.589	594	348	14	38	625	70	575	3.39e-38	149
MS.gene85146.t1	MTR_7g100430	27.111	225	162	2	24	247	112	335	1.45e-17	87.0
MS.gene85146.t1	MTR_7g100390	25.180	556	327	13	71	610	11	493	3.74e-38	149
MS.gene85146.t1	MTR_7g100390	26.616	263	188	2	2	259	11	273	8.05e-23	102
MS.gene85146.t1	MTR_7g117620	27.027	592	318	17	68	633	41	544	3.36e-36	143
MS.gene85146.t1	MTR_7g117620	31.602	231	156	2	38	267	83	312	1.19e-20	96.3
MS.gene85146.t1	MTR_2g096190	25.127	589	347	17	68	633	25	542	1.81e-34	138
MS.gene85146.t1	MTR_2g096190	27.111	225	161	2	38	260	68	291	2.88e-21	98.2
MS.gene85146.t1	MTR_4g062100	24.824	568	326	13	68	612	29	518	6.01e-34	137
MS.gene85146.t1	MTR_4g062100	25.391	256	184	4	2	251	32	286	5.44e-20	94.4
MS.gene85146.t1	MTR_8g105260	23.156	488	289	12	138	614	98	510	6.51e-30	124
MS.gene85146.t1	MTR_8g105260	27.064	218	154	5	33	247	60	275	3.90e-15	79.0
MS.gene85146.t1	MTR_1g032100	22.587	487	322	10	163	633	82	529	5.06e-28	119
MS.gene85146.t1	MTR_1g032100	27.273	220	157	2	101	319	53	270	1.25e-23	105
MS.gene85146.t1	MTR_1g032100	23.938	259	181	6	3	247	12	268	8.40e-13	71.6
MS.gene85146.t1	MTR_1g069110	25.435	460	247	11	174	610	52	438	4.10e-22	100
MS.gene85146.t1	MTR_7g099570	28.696	230	154	4	137	358	40	267	1.77e-19	93.2
MS.gene85146.t1	MTR_8g066670	25.175	286	182	5	96	367	1063	1330	1.07e-17	88.2
MS.gene85146.t1	MTR_8g072510	26.506	249	163	5	111	347	13	253	1.52e-17	86.3
MS.gene85146.t1	MTR_8g069880	26.545	275	171	8	111	372	10	266	1.81e-17	85.9
MS.gene85146.t1	MTR_8g062410	27.477	222	142	3	133	343	37	250	6.52e-17	84.3
MS.gene85146.t1	MTR_7g021090	25.714	245	162	5	111	343	12	248	8.80e-17	83.6
MS.gene85146.t1	MTR_5g085480	25.362	276	185	8	105	367	51	318	7.23e-16	81.3
MS.gene85146.t1	MTR_8g073260	26.255	259	171	4	110	356	13	263	9.39e-16	80.5
MS.gene85146.t1	MTR_3g085830	28.409	264	152	8	146	396	224	463	1.11e-15	81.3
MS.gene85146.t1	MTR_5g085480	25.362	276	185	8	105	367	211	478	1.26e-15	80.9
MS.gene85146.t1	MTR_3g105650	23.607	305	205	6	111	390	8	309	6.02e-15	77.8
MS.gene85146.t1	MTR_8g039620	26.244	221	145	4	133	343	41	253	1.38e-14	77.0
MS.gene85146.t1	MTR_8g080080	24.333	300	184	9	40	319	106	382	1.14e-13	74.7
MS.gene85146.t1	MTR_1g059930	27.064	218	139	4	137	343	43	251	2.91e-12	69.7
MS.gene85146.t1	MTR_8g467780	24.123	228	143	4	137	343	42	260	6.83e-12	68.6
MS.gene85146.t1	MTR_2g033050	25.194	258	171	8	100	343	161	410	9.51e-12	67.8
MS.gene85146.t1	MTR_8g040930	24.576	236	150	5	149	372	2	221	2.56e-11	66.2
MS.gene85146.t1	MTR_8g041240	24.576	236	150	5	149	372	2	221	2.56e-11	66.2
MS.gene85146.t1	MTR_5g043640	30.769	182	118	6	101	280	51	226	3.65e-11	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85146.t1	AT1G10340	35.484	651	333	16	3	640	2	578	1.01e-111	347
MS.gene85146.t1	AT1G10340	35.177	651	331	16	3	640	2	574	7.98e-110	342
MS.gene85146.t1	AT2G24600	31.352	673	350	15	5	640	4	601	2.04e-75	253
MS.gene85146.t1	AT2G24600	31.352	673	350	15	5	640	4	601	2.04e-75	253
MS.gene85146.t1	AT5G50140	32.136	557	319	11	115	640	7	535	2.31e-68	232
MS.gene85146.t1	AT5G54710	26.791	642	390	10	54	624	2	634	5.69e-68	234
MS.gene85146.t1	AT5G54710	28.205	390	240	7	33	390	110	491	1.10e-36	146
MS.gene85146.t1	AT2G24600	29.279	666	320	17	5	640	4	548	9.68e-65	223
MS.gene85146.t1	AT2G24600	29.279	666	320	17	5	640	4	548	9.68e-65	223
MS.gene85146.t1	AT5G54710	26.466	597	359	10	99	624	2	589	6.10e-60	211
MS.gene85146.t1	AT5G54710	28.205	390	240	7	33	390	65	446	4.07e-37	147
MS.gene85146.t1	AT1G34050	50.000	182	86	1	427	603	359	540	1.74e-50	184
MS.gene85146.t1	AT1G34050	32.087	321	208	3	1	318	17	330	1.98e-45	170
MS.gene85146.t1	AT5G54700	52.903	155	72	1	450	603	168	322	7.12e-43	158
MS.gene85146.t1	AT5G54700	35.526	152	92	2	195	345	20	166	3.10e-21	95.9
MS.gene85146.t1	AT5G54700	59.200	125	50	1	450	573	205	329	4.94e-39	150
MS.gene85146.t1	AT5G54700	27.727	220	115	6	146	345	8	203	1.45e-13	73.9
MS.gene85146.t1	AT3G12360	23.428	636	354	13	2	632	63	570	7.93e-39	151
MS.gene85146.t1	AT3G12360	23.428	636	354	13	2	632	75	582	8.30e-39	151
MS.gene85146.t1	AT2G01680	22.868	516	327	11	101	612	56	504	2.76e-34	137
MS.gene85146.t1	AT2G01680	28.249	177	123	3	39	213	97	271	5.83e-11	65.9
MS.gene85146.t1	AT2G01680	20.385	260	192	3	3	247	12	271	7.57e-11	65.5
MS.gene85146.t1	AT5G60070	25.849	530	306	13	99	613	68	525	5.35e-33	134
MS.gene85146.t1	AT5G60070	25.738	237	160	6	2	226	33	265	2.45e-17	86.3
MS.gene85146.t1	AT5G02620	26.132	574	354	18	71	627	18	538	5.45e-33	134
MS.gene85146.t1	AT5G02620	22.500	320	188	7	2	295	18	303	6.26e-15	78.6
MS.gene85146.t1	AT5G02620	26.132	574	354	18	71	627	18	538	5.45e-33	134
MS.gene85146.t1	AT5G02620	22.500	320	188	7	2	295	18	303	6.26e-15	78.6
MS.gene85146.t1	AT1G07710	25.177	564	330	15	68	613	25	514	8.09e-31	127
MS.gene85146.t1	AT1G07710	24.915	293	206	5	11	295	4	290	2.10e-18	89.4
MS.gene85146.t1	AT1G07710	25.177	564	330	15	68	613	25	514	8.09e-31	127
MS.gene85146.t1	AT1G07710	24.915	293	206	5	11	295	4	290	2.10e-18	89.4
MS.gene85146.t1	AT5G02620	25.829	573	334	18	71	627	18	515	2.70e-30	125
MS.gene85146.t1	AT5G02620	22.074	299	192	5	2	295	18	280	1.61e-15	80.1
MS.gene85146.t1	AT5G02620	25.829	573	334	18	71	627	18	515	2.70e-30	125
MS.gene85146.t1	AT5G02620	22.074	299	192	5	2	295	18	280	1.61e-15	80.1
MS.gene85146.t1	AT5G02620	25.829	573	334	18	71	627	18	515	2.70e-30	125
MS.gene85146.t1	AT5G02620	22.074	299	192	5	2	295	18	280	1.61e-15	80.1
MS.gene85146.t1	AT2G31820	26.177	531	315	16	106	611	153	631	1.30e-27	118
MS.gene85146.t1	AT2G31820	28.696	230	163	1	38	266	191	420	1.11e-20	97.1
MS.gene85146.t1	AT1G05640	24.576	472	314	9	146	611	161	596	5.29e-25	110
MS.gene85146.t1	AT1G05640	28.889	225	159	1	28	251	145	369	1.27e-19	93.6
MS.gene85146.t1	AT3G09550	26.829	328	182	5	38	343	90	381	6.60e-24	107
MS.gene85146.t1	AT3G09550	37.288	118	65	2	445	557	419	532	8.16e-16	81.6
MS.gene85146.t1	AT3G09550	26.829	328	182	5	38	343	90	381	6.60e-24	107
MS.gene85146.t1	AT3G09550	37.288	118	65	2	445	557	419	532	8.16e-16	81.6
MS.gene85146.t1	AT3G09550	26.829	328	182	5	38	343	97	388	8.53e-24	106
MS.gene85146.t1	AT3G09550	37.288	118	65	2	445	557	426	539	9.42e-16	81.3
MS.gene85146.t1	AT4G03500	25.705	319	219	5	20	324	121	435	1.58e-21	99.8
MS.gene85146.t1	AT4G03500	32.000	125	76	3	460	581	479	597	2.86e-11	67.0
MS.gene85146.t1	AT4G03500	25.705	319	219	5	20	324	98	412	2.18e-21	99.4
MS.gene85146.t1	AT4G03500	32.000	125	76	3	460	581	456	574	3.21e-11	67.0
MS.gene85146.t1	AT4G03500	25.705	319	219	5	20	324	98	412	2.18e-21	99.4
MS.gene85146.t1	AT4G03500	32.000	125	76	3	460	581	456	574	3.21e-11	67.0
MS.gene85146.t1	AT1G03670	25.743	303	202	5	38	323	108	404	1.18e-20	96.7
MS.gene85146.t1	AT1G03670	23.048	538	335	18	106	595	74	580	1.36e-16	84.0
MS.gene85146.t1	AT4G03460	24.540	326	221	6	20	324	67	388	4.20e-19	92.0
MS.gene85146.t1	AT4G03460	24.540	326	221	6	20	324	99	420	5.13e-19	91.7
MS.gene85146.t1	AT4G03450	22.546	377	238	4	1	323	36	412	3.06e-18	89.4
MS.gene85146.t1	AT4G03450	22.546	377	238	4	1	323	36	412	5.95e-18	88.2
MS.gene85146.t1	AT4G10720	28.638	213	142	2	137	339	36	248	6.42e-18	87.0
MS.gene85146.t1	AT4G10720	28.638	213	142	2	137	339	36	248	9.02e-18	87.0
MS.gene85146.t1	AT5G15500	28.916	249	155	5	111	342	9	252	1.22e-17	86.7
MS.gene85146.t1	AT5G54610	29.648	199	131	2	149	339	48	245	2.31e-16	82.4
MS.gene85146.t1	AT5G51160	26.702	191	132	2	168	350	43	233	5.67e-16	81.3
MS.gene85146.t1	AT4G14390	24.286	350	196	5	34	321	111	453	7.46e-16	81.3
MS.gene85146.t1	AT4G14390	23.499	383	224	7	1	321	98	473	1.43e-15	80.9
MS.gene85146.t1	AT4G14390	23.499	383	223	6	1	321	79	453	1.63e-15	80.9
MS.gene85146.t1	AT4G14390	23.499	383	223	6	1	321	98	472	2.20e-15	80.5
MS.gene85146.t1	AT5G54620	28.502	207	137	2	149	345	48	253	4.93e-15	78.2
MS.gene85146.t1	AT1G14500	26.872	227	155	2	142	357	41	267	2.67e-14	75.9
MS.gene85146.t1	AT4G03490	24.723	271	196	2	61	328	135	400	3.05e-14	76.6
MS.gene85146.t1	AT4G03490	24.723	271	196	2	61	328	135	400	3.34e-14	76.3
MS.gene85146.t1	AT4G03490	22.029	690	414	24	1	634	1	622	3.03e-13	73.2
MS.gene85146.t1	AT4G03490	24.723	271	196	2	61	328	125	390	3.71e-14	76.3
MS.gene85146.t1	AT4G03490	22.642	583	341	21	107	634	85	612	1.43e-11	68.2
MS.gene85146.t1	AT4G03480	25.714	245	158	6	95	316	293	536	1.44e-13	74.3
MS.gene85146.t1	AT4G03480	25.714	245	158	6	95	316	296	539	1.72e-13	74.3
MS.gene85146.t1	AT4G03440	24.784	347	194	12	39	323	162	503	3.58e-13	73.2
MS.gene85146.t1	AT4G03440	24.784	347	194	12	39	323	155	496	3.65e-13	73.2
MS.gene85146.t1	AT4G03440	24.784	347	194	12	39	323	155	496	3.65e-13	73.2
MS.gene85146.t1	AT3G04140	25.246	305	182	8	40	319	106	389	6.27e-13	72.4
MS.gene85146.t1	AT4G03470	23.304	339	207	5	33	321	116	451	1.19e-12	71.6
MS.gene85146.t1	AT1G14480	27.193	228	154	3	142	357	41	268	2.83e-12	69.7
MS.gene85146.t1	AT3G01750	21.795	312	206	6	108	381	144	455	1.34e-11	68.2
MS.gene85146.t1	AT3G01750	21.795	312	206	6	108	381	144	455	1.34e-11	68.2
MS.gene85146.t1	AT4G05040	22.460	374	228	6	1	316	81	450	4.21e-11	66.2
MS.gene85146.t1	AT4G05040	22.460	374	228	6	1	316	81	450	4.21e-11	66.2
MS.gene85146.t1	AT4G05040	22.460	374	228	6	1	316	81	450	4.21e-11	66.2
MS.gene85146.t1	AT4G05040	22.460	374	228	6	1	316	81	450	4.21e-11	66.2
MS.gene85146.t1	AT4G05040	22.460	374	228	6	1	316	81	450	4.21e-11	66.2
MS.gene85146.t1	AT4G05040	22.460	374	228	6	1	316	81	450	4.21e-11	66.2
MS.gene85146.t1	AT4G05040	22.460	374	228	6	1	316	81	450	4.21e-11	66.2

Find 117 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGCATCTTGCAGTGATTA+AGG	0.225635	2.4:+3430681	MS.gene85146:CDS
GCACTTGGTGGTGGCTTTCT+AGG	0.247558	2.4:+3432127	MS.gene85146:CDS
TTGCATCATGCTTGTTATAA+AGG	0.260488	2.4:+3430580	MS.gene85146:CDS
TTACACCTTGCTTCCAAGTA+TGG	0.266322	2.4:+3429657	MS.gene85146:CDS
TACCTCTCATTGGTCCTTCT+TGG	0.285786	2.4:-3431847	None:intergenic
GTTGGTGACGCGTAGAAATA+TGG	0.292707	2.4:-3431635	None:intergenic
GTCTTACATCTTGCAGCTTC+AGG	0.296983	2.4:+3430889	MS.gene85146:CDS
CATTTCAATGCAACCATACT+TGG	0.318111	2.4:-3429670	None:intergenic
ACTATTGACCATTGCTCATA+AGG	0.354887	2.4:+3432000	MS.gene85146:CDS
CTTCATCTGGCTGTGAGATA+TGG	0.363162	2.4:+3430784	MS.gene85146:CDS
TGCCTACAAGCCTCATGAAT+TGG	0.364385	2.4:-3429756	None:intergenic
AATGGAAGTGCTTACTGAAA+TGG	0.380266	2.4:+3431511	MS.gene85146:CDS
TGGCAGATTTCTTGATCAAC+AGG	0.393186	2.4:+3431264	MS.gene85146:CDS
ACTGTCTCTTCTTCTCTTGT+GGG	0.398158	2.4:-3430760	None:intergenic
TACTGTCTCTTCTTCTCTTG+TGG	0.399548	2.4:-3430761	None:intergenic
CTCCAATTCATGAGGCTTGT+AGG	0.414812	2.4:+3429754	MS.gene85146:CDS
AAGAAACACTATTGTATTAG+TGG	0.415812	2.4:+3431766	MS.gene85146:CDS
ATATGGATGTTATGATGCTT+TGG	0.421848	2.4:+3430801	MS.gene85146:CDS
GCAGTGGCATTTATGTCAAC+TGG	0.422609	2.4:+3432034	MS.gene85146:CDS
AGACATTCTTGATCAGAGTA+TGG	0.425608	2.4:+3431334	MS.gene85146:CDS
AAGGTATATACCGAAGGAAA+TGG	0.426038	2.4:+3431493	MS.gene85146:CDS
TTGCATCTTGCAGTGATTAA+GGG	0.431378	2.4:+3430682	MS.gene85146:CDS
CATTGCATCTCTTGATTATG+TGG	0.432210	2.4:-3432085	None:intergenic
TTCTCAGCAGAAACCATGTC+AGG	0.439348	2.4:-3429717	None:intergenic
CCATTGCTCATAAGGTTATG+TGG	0.439545	2.4:+3432008	MS.gene85146:CDS
TTCCAAGTTCCTGCAGTAAG+AGG	0.441807	2.4:+3429963	MS.gene85146:CDS
CTGGCTATGTTGCAGCTACA+TGG	0.442108	2.4:+3432053	MS.gene85146:CDS
TTGTCTATGTAGATGTTGTC+AGG	0.443159	2.4:+3430497	MS.gene85146:intron
GTAAGCACTTCCATTTCCTT+CGG	0.451237	2.4:-3431503	None:intergenic
AAAAGAAAGGGAAGCGGAGA+CGG	0.453963	2.4:+3432217	MS.gene85146:CDS
CAAGTATGGTTGCATTGAAA+TGG	0.461985	2.4:+3429671	MS.gene85146:CDS
AACAATGACTACAGATAAAG+AGG	0.462217	2.4:-3431936	None:intergenic
CGCGTAGAAATATGGATTAT+AGG	0.462284	2.4:-3431627	None:intergenic
GCCCTCTTACTGCAGGAACT+TGG	0.463080	2.4:-3429965	None:intergenic
ATTCTCCCTCACCAACATCT+AGG	0.464972	2.4:+3431456	MS.gene85146:CDS
AGAAGAGACAGTACTTCATC+TGG	0.467668	2.4:+3430771	MS.gene85146:CDS
CAAAAGTGGATATTAATACT+AGG	0.468606	2.4:+3431288	MS.gene85146:CDS
GTTGAATCTATCTGAGATTG+TGG	0.470881	2.4:+3429908	MS.gene85146:CDS
AGCTTCAGGCGGTCGCCATA+AGG	0.472412	2.4:+3430903	MS.gene85146:CDS
GTGCACAGACAAGATAGATA+TGG	0.475273	2.4:+3430859	MS.gene85146:CDS
TTTCACTATGCTTGAAAATG+TGG	0.476317	2.4:-3429583	None:intergenic
TTGAATCTATCTGAGATTGT+GGG	0.482370	2.4:+3429909	MS.gene85146:CDS
TGCTTGTTGAACACTGGCTT+AGG	0.483216	2.4:+3432176	MS.gene85146:CDS
TACTTGGAAGCAAGGTGTAA+TGG	0.487753	2.4:-3429654	None:intergenic
TTCAAGTGATCGACGAGAAA+GGG	0.488121	2.4:+3430548	MS.gene85146:CDS
ACCATTGATATACCTCTCAT+TGG	0.496126	2.4:-3431857	None:intergenic
TTAAGCTTGCAAGCAGCTGT+TGG	0.499976	2.4:-3429819	None:intergenic
AACACTGGCTTAGGAAATCC+AGG	0.505473	2.4:+3432185	MS.gene85146:CDS
ATTAATACTAGGAATGATGA+AGG	0.509114	2.4:+3431299	MS.gene85146:CDS
CTCTCACTTACATGAGGCTG+AGG	0.510399	2.4:-3431590	None:intergenic
TCGATCACTTGAATAAGATC+TGG	0.516330	2.4:-3430538	None:intergenic
ATTCAAGTGATCGACGAGAA+AGG	0.519519	2.4:+3430547	MS.gene85146:CDS
CGGAACTGCTGAATCAGATA+AGG	0.521722	2.4:+3432237	MS.gene85146:CDS
CTTGATCAACAGGACAAAAG+TGG	0.523539	2.4:+3431274	MS.gene85146:CDS
GTGCTACTAGCACTTGGTGG+TGG	0.525873	2.4:+3432118	MS.gene85146:CDS
AGAAAGAAAAGAAAGGGAAG+CGG	0.530971	2.4:+3432211	MS.gene85146:CDS
GTGTGATGCTTGTTGAACAC+TGG	0.535308	2.4:+3432170	MS.gene85146:CDS
TTCTTGGTGAGAGTTGCCTA+TGG	0.538930	2.4:+3430826	MS.gene85146:CDS
AGGTGGAGAAAGAAAAGAAA+GGG	0.545486	2.4:+3432205	MS.gene85146:CDS
ATGGTCAATAGTATTGTTTG+TGG	0.546036	2.4:-3431989	None:intergenic
CCTTCTTGGTACACACCACC+AGG	0.547653	2.4:-3431833	None:intergenic
CACATAATCAAGAGATGCAA+TGG	0.548642	2.4:+3432086	MS.gene85146:CDS
ATTGTGGGGCAAGAAGTAGC+AGG	0.549345	2.4:+3429924	MS.gene85146:CDS
CCTGTGTGCCCTCTTACTGC+AGG	0.553125	2.4:-3429972	None:intergenic
TTAATCACTGCAAGATGCAA+TGG	0.554804	2.4:-3430679	None:intergenic
TGCATCTTGCAGTGATTAAG+GGG	0.555428	2.4:+3430683	MS.gene85146:CDS
TAAGGTTATGTGGGTTGCAG+TGG	0.558426	2.4:+3432018	MS.gene85146:CDS
TCCAAGTTCCTGCAGTAAGA+GGG	0.561483	2.4:+3429964	MS.gene85146:CDS
CTAGGATTTCTTCGACGCAA+AGG	0.566261	2.4:+3431474	MS.gene85146:CDS
CAAGGCACATTTGTCAGAGA+TGG	0.566647	2.4:+3431380	MS.gene85146:CDS
GTAAGTGAGAGATTTGAGAA+TGG	0.567318	2.4:+3431602	MS.gene85146:CDS
TGCAACCATACTTGGAAGCA+AGG	0.569404	2.4:-3429662	None:intergenic
GCTGCTGGTATTAATCCACC+TGG	0.571700	2.4:+3431815	MS.gene85146:CDS
GTGCACTAGATTTGTACCAT+AGG	0.574161	2.4:-3430842	None:intergenic
GTTGTGCTACTAGCACTTGG+TGG	0.579135	2.4:+3432115	MS.gene85146:CDS
CAAAAGCATCATCGTAACAA+AGG	0.580235	2.4:+3431671	MS.gene85146:CDS
CAGGTGGAGAAAGAAAAGAA+AGG	0.580805	2.4:+3432204	MS.gene85146:CDS
TGTTAGATTATGCCCTGACA+TGG	0.583786	2.4:+3429704	MS.gene85146:CDS
ACCAATGAGAGGTATATCAA+TGG	0.585047	2.4:+3431856	MS.gene85146:CDS
TTTCAAAGCTCTTATCTACA+GGG	0.585554	2.4:+3432277	MS.gene85146:CDS
TCAGGGAGCTGTTGAACAAG+TGG	0.588648	2.4:+3430515	MS.gene85146:CDS
TAGTCAAGGTCATCTTGACT+TGG	0.591019	2.4:+3429875	MS.gene85146:CDS
GCTTTACAATACAACAACAA+TGG	0.591686	2.4:+3430649	MS.gene85146:CDS
TCTGTTGTGCTACTAGCACT+TGG	0.599953	2.4:+3432112	MS.gene85146:CDS
TACTACCACAAAATAGTCGA+GGG	0.601617	2.4:-3430716	None:intergenic
CATTGCTCATAAGGTTATGT+GGG	0.602691	2.4:+3432009	MS.gene85146:CDS
GACGCAAAGGTATATACCGA+AGG	0.607678	2.4:+3431487	MS.gene85146:CDS
ATGAGAGGTATATCAATGGT+TGG	0.607766	2.4:+3431860	MS.gene85146:CDS
TCTCAGATAGATTCAACAAG+AGG	0.608966	2.4:-3429902	None:intergenic
AAGGTGTAATGGAGTGCTGA+AGG	0.609010	2.4:-3429643	None:intergenic
GGCACATTTGTCAGAGATGG+TGG	0.609271	2.4:+3431383	MS.gene85146:CDS
GTCGAAGAAATCCTAGATGT+TGG	0.613701	2.4:-3431467	None:intergenic
TTACATCTTGCAGCTTCAGG+CGG	0.614976	2.4:+3430892	MS.gene85146:CDS
TGGCTATGTTGCAGCTACAT+GGG	0.618021	2.4:+3432054	MS.gene85146:CDS
CCACATAACCTTATGAGCAA+TGG	0.619714	2.4:-3432008	None:intergenic
AGAAATCCTAGATGTTGGTG+AGG	0.626960	2.4:-3431462	None:intergenic
CCTGGTGGTGTGTACCAAGA+AGG	0.628111	2.4:+3431833	MS.gene85146:CDS
CTATCATGAGATGCACAAAG+AGG	0.630062	2.4:+3431730	MS.gene85146:CDS
TTACTACCACAAAATAGTCG+AGG	0.634588	2.4:-3430717	None:intergenic
GAAATCCTAGATGTTGGTGA+GGG	0.638433	2.4:-3431461	None:intergenic
GCAGAAAATAGACAGCTACA+AGG	0.645532	2.4:+3431362	MS.gene85146:CDS
TTTATAACAAGCATGATGCA+AGG	0.645651	2.4:-3430578	None:intergenic
TACCAAGAAGGACCAATGAG+AGG	0.646349	2.4:+3431845	MS.gene85146:CDS
TGTCTATGTAGATGTTGTCA+GGG	0.648564	2.4:+3430498	MS.gene85146:intron
TTGTGGGGCAAGAAGTAGCA+GGG	0.651054	2.4:+3429925	MS.gene85146:CDS
TCAAATCTCTCACTTACATG+AGG	0.653146	2.4:-3431596	None:intergenic
CATGGAGACTCCAATTCATG+AGG	0.658286	2.4:+3429746	MS.gene85146:CDS
CCTGCAGTAAGAGGGCACAC+AGG	0.664743	2.4:+3429972	MS.gene85146:CDS
TCTCAGCAGAAACCATGTCA+GGG	0.665903	2.4:-3429716	None:intergenic
ACCAGCAGCAAAAGTTACAG+TGG	0.670740	2.4:-3431801	None:intergenic
TTGTAGGCAAGAGAATGTCA+AGG	0.670867	2.4:+3429770	MS.gene85146:CDS
TTAATACTAGGAATGATGAA+GGG	0.670892	2.4:+3431300	MS.gene85146:CDS
TCTTGGTACACACCACCAGG+TGG	0.673788	2.4:-3431830	None:intergenic
GAAATGTGAATCATACCAAG+AGG	0.674322	2.4:+3431702	MS.gene85146:CDS
TGAATCTATCTGAGATTGTG+GGG	0.685330	2.4:+3429910	MS.gene85146:CDS
GCTGGTATTAATCCACCTGG+TGG	0.710773	2.4:+3431818	MS.gene85146:CDS
ACTGGCTTAGGAAATCCAGG+TGG	0.784007	2.4:+3432188	MS.gene85146:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCAATAAGAAAATGCAATA+TGG	-	chr2.4:3431015-3431034	None:intergenic	20.0%
!!	TTTGATCACTAATAATAAGA+AGG	+	chr2.4:3430421-3430440	MS.gene85146:intron	20.0%
!	AAACAGTATATCAGAAAATC+AGG	+	chr2.4:3431143-3431162	MS.gene85146:intron	25.0%
!	AAGAAACACTATTGTATTAG+TGG	+	chr2.4:3431766-3431785	MS.gene85146:CDS	25.0%
!	AAGTGAAAAAGAAGAAGATT+CGG	-	chr2.4:3431420-3431439	None:intergenic	25.0%
!	AATTGACGTTTAATTAACAG+AGG	-	chr2.4:3430185-3430204	None:intergenic	25.0%
!	ATTAATACTAGGAATGATGA+AGG	+	chr2.4:3431299-3431318	MS.gene85146:CDS	25.0%
!	CAAAAGTGGATATTAATACT+AGG	+	chr2.4:3431288-3431307	MS.gene85146:CDS	25.0%
!	TTAATACTAGGAATGATGAA+GGG	+	chr2.4:3431300-3431319	MS.gene85146:CDS	25.0%
!!!	AAAAAAGCACTTTTACAAGT+AGG	-	chr2.4:3429846-3429865	None:intergenic	25.0%
!!!	AATTTTTTGTCTGTAGTATC+TGG	+	chr2.4:3431170-3431189	MS.gene85146:intron	25.0%
!!!	TGAACTTATATTTTAGCCTT+AGG	+	chr2.4:3430065-3430084	MS.gene85146:intron	25.0%
!!!	TTTTGTTTCGCTAAATTAGT+TGG	-	chr2.4:3431656-3431675	None:intergenic	25.0%
	AACAATGACTACAGATAAAG+AGG	-	chr2.4:3431939-3431958	None:intergenic	30.0%
	AGCATAGTGAAAAAAAGAGA+AGG	+	chr2.4:3429594-3429613	MS.gene85146:CDS	30.0%
	ATATGGATGTTATGATGCTT+TGG	+	chr2.4:3430801-3430820	MS.gene85146:CDS	30.0%
	ATGGTCAATAGTATTGTTTG+TGG	-	chr2.4:3431992-3432011	None:intergenic	30.0%
	CACATAAATTTCTCACCTTA+TGG	-	chr2.4:3430921-3430940	None:intergenic	30.0%
	CACTAATAATAAGAAGGTGA+TGG	+	chr2.4:3430427-3430446	MS.gene85146:intron	30.0%
	CCTTAGGAGATATTAATTCA+AGG	+	chr2.4:3430081-3430100	MS.gene85146:intron	30.0%
	CCTTGAATTAATATCTCCTA+AGG	-	chr2.4:3430084-3430103	None:intergenic	30.0%
	GATGTAAGTTATCTCCTAAA+CGG	+	chr2.4:3430470-3430489	MS.gene85146:intron	30.0%
	GCATAGTGAAAAAAAGAGAA+GGG	+	chr2.4:3429595-3429614	MS.gene85146:CDS	30.0%
	GCTTTACAATACAACAACAA+TGG	+	chr2.4:3430649-3430668	MS.gene85146:CDS	30.0%
	GGTTCTATAATCTTTGTAGA+TGG	+	chr2.4:3431244-3431263	MS.gene85146:intron	30.0%
	GTAACAATTGATATACCTAG+TGG	+	chr2.4:3430340-3430359	MS.gene85146:intron	30.0%
	TTCTGCTGAGAATAAAAACA+TGG	+	chr2.4:3429728-3429747	MS.gene85146:CDS	30.0%
	TTGGAACTTAAGGACTAAAA+CGG	+	chr2.4:3430379-3430398	MS.gene85146:intron	30.0%
	TTTATAACAAGCATGATGCA+AGG	-	chr2.4:3430581-3430600	None:intergenic	30.0%
	TTTCAAAGCTCTTATCTACA+GGG	+	chr2.4:3432277-3432296	MS.gene85146:CDS	30.0%
	TTTCACTATGCTTGAAAATG+TGG	-	chr2.4:3429586-3429605	None:intergenic	30.0%
!	GTTTGTGGTTTTCTTCTAAA+TGG	-	chr2.4:3431977-3431996	None:intergenic	30.0%
!	TTGAATCTATCTGAGATTGT+GGG	+	chr2.4:3429909-3429928	MS.gene85146:CDS	30.0%
!	TTTGCACAATTTGGAACTTA+AGG	+	chr2.4:3430369-3430388	MS.gene85146:intron	30.0%
!	TTTTAACGCTATAGTCTATG+TGG	-	chr2.4:3430285-3430304	None:intergenic	30.0%
!	TTTTCAAAGCTCTTATCTAC+AGG	+	chr2.4:3432276-3432295	MS.gene85146:CDS	30.0%
!!	CAATTCAATAGTTCTGATCA+TGG	+	chr2.4:3431223-3431242	MS.gene85146:intron	30.0%
!!	TTGCATCATGCTTGTTATAA+AGG	+	chr2.4:3430580-3430599	MS.gene85146:CDS	30.0%
!!!	AAGGACATTTTGAGATTGTA+TGG	+	chr2.4:3430599-3430618	MS.gene85146:CDS	30.0%
!!!	TTATGATGCTTTGGTTTTCT+TGG	+	chr2.4:3430810-3430829	MS.gene85146:CDS	30.0%
!!!	TTTTTTGTAGCTTGTAGTCA+AGG	+	chr2.4:3429861-3429880	MS.gene85146:CDS	30.0%
	AAGGTATATACCGAAGGAAA+TGG	+	chr2.4:3431493-3431512	MS.gene85146:CDS	35.0%
	ACATAGACAATCAACCGTTT+AGG	-	chr2.4:3430487-3430506	None:intergenic	35.0%
	ACCATTGATATACCTCTCAT+TGG	-	chr2.4:3431860-3431879	None:intergenic	35.0%
	ACTATTGACCATTGCTCATA+AGG	+	chr2.4:3432000-3432019	MS.gene85146:CDS	35.0%
	AGAAAGAAAAGAAAGGGAAG+CGG	+	chr2.4:3432211-3432230	MS.gene85146:CDS	35.0%
	AGACATTCTTGATCAGAGTA+TGG	+	chr2.4:3431334-3431353	MS.gene85146:CDS	35.0%
	AGGTGGAGAAAGAAAAGAAA+GGG	+	chr2.4:3432205-3432224	MS.gene85146:CDS	35.0%
	ATTGCATCTTGCAGTGATTA+AGG	+	chr2.4:3430681-3430700	MS.gene85146:CDS	35.0%
	CAAGTATGGTTGCATTGAAA+TGG	+	chr2.4:3429671-3429690	MS.gene85146:CDS	35.0%
	CACATAATCAAGAGATGCAA+TGG	+	chr2.4:3432086-3432105	MS.gene85146:CDS	35.0%
	CATCGTAACAAAGGAAAAAC+TGG	+	chr2.4:3431680-3431699	MS.gene85146:CDS	35.0%
	CATTGCATCTCTTGATTATG+TGG	-	chr2.4:3432088-3432107	None:intergenic	35.0%
	CATTGCTCATAAGGTTATGT+GGG	+	chr2.4:3432009-3432028	MS.gene85146:CDS	35.0%
	CATTTCAATGCAACCATACT+TGG	-	chr2.4:3429673-3429692	None:intergenic	35.0%
	GAAATGTGAATCATACCAAG+AGG	+	chr2.4:3431702-3431721	MS.gene85146:CDS	35.0%
	GTAAGTGAGAGATTTGAGAA+TGG	+	chr2.4:3431602-3431621	MS.gene85146:CDS	35.0%
	GTGTTTCAATAGAGCGAATT+AGG	+	chr2.4:3430127-3430146	MS.gene85146:intron	35.0%
	TACTACCACAAAATAGTCGA+GGG	-	chr2.4:3430719-3430738	None:intergenic	35.0%
	TCAAATCTCTCACTTACATG+AGG	-	chr2.4:3431599-3431618	None:intergenic	35.0%
	TCGATCACTTGAATAAGATC+TGG	-	chr2.4:3430541-3430560	None:intergenic	35.0%
	TCTCAGATAGATTCAACAAG+AGG	-	chr2.4:3429905-3429924	None:intergenic	35.0%
	TGGACACAATTTGCACAATT+TGG	+	chr2.4:3430360-3430379	MS.gene85146:intron	35.0%
	TGTCTATGTAGATGTTGTCA+GGG	+	chr2.4:3430498-3430517	MS.gene85146:intron	35.0%
	TTAATCACTGCAAGATGCAA+TGG	-	chr2.4:3430682-3430701	None:intergenic	35.0%
	TTACTACCACAAAATAGTCG+AGG	-	chr2.4:3430720-3430739	None:intergenic	35.0%
	TTGCATCTTGCAGTGATTAA+GGG	+	chr2.4:3430682-3430701	MS.gene85146:CDS	35.0%
	TTGTCTATGTAGATGTTGTC+AGG	+	chr2.4:3430497-3430516	MS.gene85146:intron	35.0%
!	ACCAATGAGAGGTATATCAA+TGG	+	chr2.4:3431856-3431875	MS.gene85146:CDS	35.0%
!	ATGAGAGGTATATCAATGGT+TGG	+	chr2.4:3431860-3431879	MS.gene85146:CDS	35.0%
!	CGCGTAGAAATATGGATTAT+AGG	-	chr2.4:3431630-3431649	None:intergenic	35.0%
!	CTCATGATAGTTTTTCCTCT+TGG	-	chr2.4:3431720-3431739	None:intergenic	35.0%
!	GATTCAGTGCTTTTGCTAAA+CGG	-	chr2.4:3431563-3431582	None:intergenic	35.0%
!	GTTGAATCTATCTGAGATTG+TGG	+	chr2.4:3429908-3429927	MS.gene85146:CDS	35.0%
!	TGAATCTATCTGAGATTGTG+GGG	+	chr2.4:3429910-3429929	MS.gene85146:CDS	35.0%
!	TGGTGAAACATCAGCTTTTA+AGG	+	chr2.4:3431880-3431899	MS.gene85146:CDS	35.0%
!	TTTTAATCTGTGCAACGTAC+GGG	+	chr2.4:3430965-3430984	MS.gene85146:intron	35.0%
!!	AAGTTGGTGTCAACTTATTC+TGG	+	chr2.4:3430232-3430251	MS.gene85146:intron	35.0%
!!	AATGGAAGTGCTTACTGAAA+TGG	+	chr2.4:3431511-3431530	MS.gene85146:CDS	35.0%
!!	CAAAAGCATCATCGTAACAA+AGG	+	chr2.4:3431671-3431690	MS.gene85146:CDS	35.0%
!!	GTTTTAATCTGTGCAACGTA+CGG	+	chr2.4:3430964-3430983	MS.gene85146:intron	35.0%
!!!	TGAAGGTATCATCTGTTTTC+TGG	-	chr2.4:3429629-3429648	None:intergenic	35.0%
	ACTGTCTCTTCTTCTCTTGT+GGG	-	chr2.4:3430763-3430782	None:intergenic	40.0%
	ACTTAGACAACTTAGGCTCT+AGG	-	chr2.4:3430216-3430235	None:intergenic	40.0%
	AGAAATCCTAGATGTTGGTG+AGG	-	chr2.4:3431465-3431484	None:intergenic	40.0%
	AGAAGAGACAGTACTTCATC+TGG	+	chr2.4:3430771-3430790	MS.gene85146:CDS	40.0%
	AGAGCCTAAGTTGTCTAAGT+TGG	+	chr2.4:3430216-3430235	MS.gene85146:intron	40.0%
	ATAGAGCGAATTAGGCAAAC+AGG	+	chr2.4:3430135-3430154	MS.gene85146:intron	40.0%
	ATTCAAGTGATCGACGAGAA+AGG	+	chr2.4:3430547-3430566	MS.gene85146:CDS	40.0%
	CAGGTGGAGAAAGAAAAGAA+AGG	+	chr2.4:3432204-3432223	MS.gene85146:CDS	40.0%
	CCACATAACCTTATGAGCAA+TGG	-	chr2.4:3432011-3432030	None:intergenic	40.0%
	CCATTGCTCATAAGGTTATG+TGG	+	chr2.4:3432008-3432027	MS.gene85146:CDS	40.0%
	CTATCATGAGATGCACAAAG+AGG	+	chr2.4:3431730-3431749	MS.gene85146:CDS	40.0%
	CTTGATCAACAGGACAAAAG+TGG	+	chr2.4:3431274-3431293	MS.gene85146:CDS	40.0%
	GACACCAACTTAGACAACTT+AGG	-	chr2.4:3430223-3430242	None:intergenic	40.0%
	GCAGAAAATAGACAGCTACA+AGG	+	chr2.4:3431362-3431381	MS.gene85146:CDS	40.0%
	GTAAGCACTTCCATTTCCTT+CGG	-	chr2.4:3431506-3431525	None:intergenic	40.0%
	GTCGAAGAAATCCTAGATGT+TGG	-	chr2.4:3431470-3431489	None:intergenic	40.0%
	GTGCACAGACAAGATAGATA+TGG	+	chr2.4:3430859-3430878	MS.gene85146:CDS	40.0%
	GTGCACTAGATTTGTACCAT+AGG	-	chr2.4:3430845-3430864	None:intergenic	40.0%
	TACTGTCTCTTCTTCTCTTG+TGG	-	chr2.4:3430764-3430783	None:intergenic	40.0%
	TACTTGGAAGCAAGGTGTAA+TGG	-	chr2.4:3429657-3429676	None:intergenic	40.0%
	TAGTCAAGGTCATCTTGACT+TGG	+	chr2.4:3429875-3429894	MS.gene85146:CDS	40.0%
	TGCATCTTGCAGTGATTAAG+GGG	+	chr2.4:3430683-3430702	MS.gene85146:CDS	40.0%
	TGGCAGATTTCTTGATCAAC+AGG	+	chr2.4:3431264-3431283	MS.gene85146:CDS	40.0%
	TGTTAGATTATGCCCTGACA+TGG	+	chr2.4:3429704-3429723	MS.gene85146:CDS	40.0%
	TTACACCTTGCTTCCAAGTA+TGG	+	chr2.4:3429657-3429676	MS.gene85146:CDS	40.0%
	TTCAAGTGATCGACGAGAAA+GGG	+	chr2.4:3430548-3430567	MS.gene85146:CDS	40.0%
	TTGTAGGCAAGAGAATGTCA+AGG	+	chr2.4:3429770-3429789	MS.gene85146:CDS	40.0%
	TTGTGCAAATTGTGTCCACT+AGG	-	chr2.4:3430358-3430377	None:intergenic	40.0%
	TTTAATCTGTGCAACGTACG+GGG	+	chr2.4:3430966-3430985	MS.gene85146:intron	40.0%
!	CTTTCTTTTCTTTCTCCACC+TGG	-	chr2.4:3432206-3432225	None:intergenic	40.0%
!	GAAATCCTAGATGTTGGTGA+GGG	-	chr2.4:3431464-3431483	None:intergenic	40.0%
!	TCAAGGCTGAATTGCACTTT+TGG	+	chr2.4:3430098-3430117	MS.gene85146:intron	40.0%
	AAAAGAAAGGGAAGCGGAGA+CGG	+	chr2.4:3432217-3432236	MS.gene85146:CDS	45.0%
	ACCAGCAGCAAAAGTTACAG+TGG	-	chr2.4:3431804-3431823	None:intergenic	45.0%
	ATTCTCCCTCACCAACATCT+AGG	+	chr2.4:3431456-3431475	MS.gene85146:CDS	45.0%
	CAAGGCACATTTGTCAGAGA+TGG	+	chr2.4:3431380-3431399	MS.gene85146:CDS	45.0%
	CATGGAGACTCCAATTCATG+AGG	+	chr2.4:3429746-3429765	MS.gene85146:CDS	45.0%
	CGGAACTGCTGAATCAGATA+AGG	+	chr2.4:3432237-3432256	MS.gene85146:CDS	45.0%
	CTAGGATTTCTTCGACGCAA+AGG	+	chr2.4:3431474-3431493	MS.gene85146:CDS	45.0%
	CTCCAATTCATGAGGCTTGT+AGG	+	chr2.4:3429754-3429773	MS.gene85146:CDS	45.0%
	CTTCATCTGGCTGTGAGATA+TGG	+	chr2.4:3430784-3430803	MS.gene85146:CDS	45.0%
	GCAGTGGCATTTATGTCAAC+TGG	+	chr2.4:3432034-3432053	MS.gene85146:CDS	45.0%
	GTCTTACATCTTGCAGCTTC+AGG	+	chr2.4:3430889-3430908	MS.gene85146:CDS	45.0%
	TACCAAGAAGGACCAATGAG+AGG	+	chr2.4:3431845-3431864	MS.gene85146:CDS	45.0%
	TACCTCTCATTGGTCCTTCT+TGG	-	chr2.4:3431850-3431869	None:intergenic	45.0%
	TCCAAGTTCCTGCAGTAAGA+GGG	+	chr2.4:3429964-3429983	MS.gene85146:CDS	45.0%
	TCTCAGCAGAAACCATGTCA+GGG	-	chr2.4:3429719-3429738	None:intergenic	45.0%
	TGCAACCATACTTGGAAGCA+AGG	-	chr2.4:3429665-3429684	None:intergenic	45.0%
	TGCCTACAAGCCTCATGAAT+TGG	-	chr2.4:3429759-3429778	None:intergenic	45.0%
	TGGCTATGTTGCAGCTACAT+GGG	+	chr2.4:3432054-3432073	MS.gene85146:CDS	45.0%
	TTAAGCTTGCAAGCAGCTGT+TGG	-	chr2.4:3429822-3429841	None:intergenic	45.0%
	TTACATCTTGCAGCTTCAGG+CGG	+	chr2.4:3430892-3430911	MS.gene85146:CDS	45.0%
	TTCCAAGTTCCTGCAGTAAG+AGG	+	chr2.4:3429963-3429982	MS.gene85146:CDS	45.0%
	TTCTCAGCAGAAACCATGTC+AGG	-	chr2.4:3429720-3429739	None:intergenic	45.0%
	TTCTTGGTGAGAGTTGCCTA+TGG	+	chr2.4:3430826-3430845	MS.gene85146:CDS	45.0%
!	CTCAACCCTCGACTATTTTG+TGG	+	chr2.4:3430711-3430730	MS.gene85146:CDS	45.0%
!	GACGCAAAGGTATATACCGA+AGG	+	chr2.4:3431487-3431506	MS.gene85146:CDS	45.0%
!	GGCTGAATTGCACTTTTGGT+AGG	+	chr2.4:3430102-3430121	MS.gene85146:intron	45.0%
!	GTGTGATGCTTGTTGAACAC+TGG	+	chr2.4:3432170-3432189	MS.gene85146:CDS	45.0%
!	TAAGGTTATGTGGGTTGCAG+TGG	+	chr2.4:3432018-3432037	MS.gene85146:CDS	45.0%
!	TCAGTGCTTTTGCTAAACGG+TGG	-	chr2.4:3431560-3431579	None:intergenic	45.0%
!	TCTGTTGTGCTACTAGCACT+TGG	+	chr2.4:3432112-3432131	MS.gene85146:CDS	45.0%
!	TGCTTTTGCTAAACGGTGGA+GGG	-	chr2.4:3431556-3431575	None:intergenic	45.0%
!!	AACACTGGCTTAGGAAATCC+AGG	+	chr2.4:3432185-3432204	MS.gene85146:CDS	45.0%
!!	AAGGTGTAATGGAGTGCTGA+AGG	-	chr2.4:3429646-3429665	None:intergenic	45.0%
!!	CAAGGTTTTGATGTTGCTGC+TGG	+	chr2.4:3429788-3429807	MS.gene85146:CDS	45.0%
!!	GTTGGTGACGCGTAGAAATA+TGG	-	chr2.4:3431638-3431657	None:intergenic	45.0%
!!	TGCTTGTTGAACACTGGCTT+AGG	+	chr2.4:3432176-3432195	MS.gene85146:CDS	45.0%
	ATTGTGGGGCAAGAAGTAGC+AGG	+	chr2.4:3429924-3429943	MS.gene85146:CDS	50.0%
	CTCTCACTTACATGAGGCTG+AGG	-	chr2.4:3431593-3431612	None:intergenic	50.0%
	CTGGCTATGTTGCAGCTACA+TGG	+	chr2.4:3432053-3432072	MS.gene85146:CDS	50.0%
	GCTGCTGGTATTAATCCACC+TGG	+	chr2.4:3431815-3431834	MS.gene85146:CDS	50.0%
	GCTGGTATTAATCCACCTGG+TGG	+	chr2.4:3431818-3431837	MS.gene85146:CDS	50.0%
	GGCACATTTGTCAGAGATGG+TGG	+	chr2.4:3431383-3431402	MS.gene85146:CDS	50.0%
	TCAGGGAGCTGTTGAACAAG+TGG	+	chr2.4:3430515-3430534	MS.gene85146:CDS	50.0%
	TTGTGGGGCAAGAAGTAGCA+GGG	+	chr2.4:3429925-3429944	MS.gene85146:CDS	50.0%
!	ACTGGCTTAGGAAATCCAGG+TGG	+	chr2.4:3432188-3432207	MS.gene85146:CDS	50.0%
!	GCCACTGTAACTTTTGCTGC+TGG	+	chr2.4:3431800-3431819	MS.gene85146:CDS	50.0%
!	GTGCTTTTGCTAAACGGTGG+AGG	-	chr2.4:3431557-3431576	None:intergenic	50.0%
!	GTTGTGCTACTAGCACTTGG+TGG	+	chr2.4:3432115-3432134	MS.gene85146:CDS	50.0%
	CCTTCTTGGTACACACCACC+AGG	-	chr2.4:3431836-3431855	None:intergenic	55.0%
	GCCCTCTTACTGCAGGAACT+TGG	-	chr2.4:3429968-3429987	None:intergenic	55.0%
	TCTTGGTACACACCACCAGG+TGG	-	chr2.4:3431833-3431852	None:intergenic	55.0%
!	CAGTAAGAGGGCACACAGGT+GGG	+	chr2.4:3429976-3429995	MS.gene85146:intron	55.0%
!	CCTGGTGGTGTGTACCAAGA+AGG	+	chr2.4:3431833-3431852	MS.gene85146:CDS	55.0%
!	GTGCTACTAGCACTTGGTGG+TGG	+	chr2.4:3432118-3432137	MS.gene85146:CDS	55.0%
!!	GCACTTGGTGGTGGCTTTCT+AGG	+	chr2.4:3432127-3432146	MS.gene85146:CDS	55.0%
	AGCTTCAGGCGGTCGCCATA+AGG	+	chr2.4:3430903-3430922	MS.gene85146:CDS	60.0%
	CCTGTGTGCCCTCTTACTGC+AGG	-	chr2.4:3429975-3429994	None:intergenic	60.0%
!	CCTGCAGTAAGAGGGCACAC+AGG	+	chr2.4:3429972-3429991	MS.gene85146:CDS	60.0%
!	GCAGTAAGAGGGCACACAGG+TGG	+	chr2.4:3429975-3429994	MS.gene85146:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	3429540	3432315	3429540	ID=MS.gene85146
chr2.4	mRNA	3429540	3432315	3429540	ID=MS.gene85146.t1;Parent=MS.gene85146
chr2.4	exon	3429540	3429993	3429540	ID=MS.gene85146.t1.exon1;Parent=MS.gene85146.t1
chr2.4	CDS	3429540	3429993	3429540	ID=cds.MS.gene85146.t1;Parent=MS.gene85146.t1
chr2.4	exon	3430509	3430924	3430509	ID=MS.gene85146.t1.exon2;Parent=MS.gene85146.t1
chr2.4	CDS	3430509	3430924	3430509	ID=cds.MS.gene85146.t1;Parent=MS.gene85146.t1
chr2.4	exon	3431263	3432315	3431263	ID=MS.gene85146.t1.exon3;Parent=MS.gene85146.t1
chr2.4	CDS	3431263	3432315	3431263	ID=cds.MS.gene85146.t1;Parent=MS.gene85146.t1

Gene Sequence

>MS.gene85146

ATGGATCAAGAGTTTTTCGATGCAATTAAGAAAAATGACATGACCACATTTTCAAGCATAGTGAAAAAAAGAGAAGGGATTCTCAACCAGAAAACAGATGATACCTTCAGCACTCCATTACACCTTGCTTCCAAGTATGGTTGCATTGAAATGGTGTCAGAGATTGTTAGATTATGCCCTGACATGGTTTCTGCTGAGAATAAAAACATGGAGACTCCAATTCATGAGGCTTGTAGGCAAGAGAATGTCAAGGTTTTGATGTTGCTGCTGGAAGTCAATCCAACAGCTGCTTGCAAGCTTAATCCTACTTGTAAAAGTGCTTTTTTTGTAGCTTGTAGTCAAGGTCATCTTGACTTGGTGAACCTCTTGTTGAATCTATCTGAGATTGTGGGGCAAGAAGTAGCAGGGTTTGATCAAGCTTGTTTCCAAGTTCCTGCAGTAAGAGGGCACACAGGTGGGAAGAAAGAGTTTTATTCACTATGTTTGCATGTTTTTTGAACAATATTTTTTAATGAGTATTGAATTTGAACTTATATTTTAGCCTTAGGAGATATTAATTCAAGGCTGAATTGCACTTTTGGTAGGTTGTGTTTCAATAGAGCGAATTAGGCAAACAGGTATTCTAAATTTTACAATTAGATTCCTCTGTTAATTAAACGTCAATTTTTTAACGCCTAGAGCCTAAGTTGTCTAAGTTGGTGTCAACTTATTCTGGCTAAGCAAGCTTCAAGACTCACGTTGACCACATAGACTATAGCGTTAAAAAATAATAGACGATTAATAGATAGACAAATTTGATTGTAACAATTGATATACCTAGTGGACACAATTTGCACAATTTGGAACTTAAGGACTAAAACGGTACAATCTTAATAGCTTAATTTGATCACTAATAATAAGAAGGTGATGGATTGAAAATCTTATATAGCTGATGTAAGTTATCTCCTAAACGGTTGATTGTCTATGTAGATGTTGTCAGGGAGCTGTTGAACAAGTGGCCAGATCTTATTCAAGTGATCGACGAGAAAGGGAATACAGCCTTGCATCATGCTTGTTATAAAGGACATTTTGAGATTGTATGGATCATACTAAGTCGCGATTCAAATCTTGCTTTACAATACAACAACAATGGTTACACTCCATTGCATCTTGCAGTGATTAAGGGGAAAGTCTCAACCCTCGACTATTTTGTGGTAGTAAGTACTGCGTACTTTCACTATCCCACAAGAGAAGAAGAGACAGTACTTCATCTGGCTGTGAGATATGGATGTTATGATGCTTTGGTTTTCTTGGTGAGAGTTGCCTATGGTACAAATCTAGTGCACAGACAAGATAGATATGGCAACACTGTCTTACATCTTGCAGCTTCAGGCGGTCGCCATAAGGTGAGAAATTTATGTGAGCTCTGAATATATTTAACTAGTGTTTTAATCTGTGCAACGTACGGGGAGATAATTTTAGTGTAATTATATCCATATTGCATTTTCTTATTGAAAAGTACTAACTGTTGTAATTTGAGAGCATTGAAATGATTTAAATACTGAAAAGTAACATTTAAGATTTTTCTAATGTTTTTTATTTTGAAATCATGACAAATGCAAGTAAACAGTATATCAGAAAATCAGGCATCAATTTTTTGTCTGTAGTATCTGGCTTTAGTACTTAAGCTCTTTCAATATCATTCAATTCAATAGTTCTGATCATGGTTCTATAATCTTTGTAGATGGCAGATTTCTTGATCAACAGGACAAAAGTGGATATTAATACTAGGAATGATGAAGGGCTCACAGCACTAGACATTCTTGATCAGAGTATGGACAGTGCAGAAAATAGACAGCTACAAGGCACATTTGTCAGAGATGGTGGTAAAAGAAGCACCGAATCTTCTTCTTTTTCACTTGAACTAGACAACATCAATTCTCCCTCACCAACATCTAGGATTTCTTCGACGCAAAGGTATATACCGAAGGAAATGGAAGTGCTTACTGAAATGGTTTCTTATGATTGTACAAGCCCTCCACCGTTTAGCAAAAGCACTGAATCTACATCACCTCAGCCTCATGTAAGTGAGAGATTTGAGAATGGAACCTATAATCCATATTTCTACGCGTCACCAACTAATTTAGCGAAACAAAAGCATCATCGTAACAAAGGAAAAACTGGAAATGTGAATCATACCAAGAGGAAAAACTATCATGAGATGCACAAAGAGGCATTACTAAATGCAAGAAACACTATTGTATTAGTGGCTGTTTTGATTGCCACTGTAACTTTTGCTGCTGGTATTAATCCACCTGGTGGTGTGTACCAAGAAGGACCAATGAGAGGTATATCAATGGTTGGTGAAACATCAGCTTTTAAGGTATTTGCAATAAGCAATATCATAGCGCTCTTCACCTCTTTATCTGTAGTCATTGTTCTTGTTAGTATCATTCCATTTAGAAGAAAACCACAAACAATACTATTGACCATTGCTCATAAGGTTATGTGGGTTGCAGTGGCATTTATGTCAACTGGCTATGTTGCAGCTACATGGGTGATTTTGCCACATAATCAAGAGATGCAATGGCTATCTGTTGTGCTACTAGCACTTGGTGGTGGCTTTCTAGGCACGATATTCATTAGTCTTAGTGTGATGCTTGTTGAACACTGGCTTAGGAAATCCAGGTGGAGAAAGAAAAGAAAGGGAAGCGGAGACGGAACTGCTGAATCAGATAAGGAAAGTGAAGATTCAGATTTTCAAAGCTCTTATCTACAGGGTTATCATTCATATTGA

Protein sequence

>MS.gene85146.t1

MDQEFFDAIKKNDMTTFSSIVKKREGILNQKTDDTFSTPLHLASKYGCIEMVSEIVRLCPDMVSAENKNMETPIHEACRQENVKVLMLLLEVNPTAACKLNPTCKSAFFVACSQGHLDLVNLLLNLSEIVGQEVAGFDQACFQVPAVRGHTDVVRELLNKWPDLIQVIDEKGNTALHHACYKGHFEIVWIILSRDSNLALQYNNNGYTPLHLAVIKGKVSTLDYFVVVSTAYFHYPTREEETVLHLAVRYGCYDALVFLVRVAYGTNLVHRQDRYGNTVLHLAASGGRHKMADFLINRTKVDINTRNDEGLTALDILDQSMDSAENRQLQGTFVRDGGKRSTESSSFSLELDNINSPSPTSRISSTQRYIPKEMEVLTEMVSYDCTSPPPFSKSTESTSPQPHVSERFENGTYNPYFYASPTNLAKQKHHRNKGKTGNVNHTKRKNYHEMHKEALLNARNTIVLVAVLIATVTFAAGINPPGGVYQEGPMRGISMVGETSAFKVFAISNIIALFTSLSVVIVLVSIIPFRRKPQTILLTIAHKVMWVAVAFMSTGYVAATWVILPHNQEMQWLSVVLLALGGGFLGTIFISLSVMLVEHWLRKSRWRKKRKGSGDGTAESDKESEDSDFQSSYLQGYHSY