AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000144

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000144.t1 MTR_5g006180 95.455 572 12 1 1 572 1 558 0.0 1115
MS.gene000144.t1 MTR_5g090110 78.307 567 72 5 8 572 7 524 0.0 884
MS.gene000144.t1 MTR_4g115780 61.176 595 202 8 1 571 101 690 0.0 664
MS.gene000144.t1 MTR_4g107920 56.863 561 207 13 19 569 10 545 0.0 573
MS.gene000144.t1 MTR_7g024390 37.228 505 290 10 29 523 9 496 7.23e-99 314
MS.gene000144.t1 MTR_7g024580 37.426 505 289 10 29 523 9 496 1.89e-98 313
MS.gene000144.t1 MTR_4g130540 36.853 502 296 10 29 523 8 495 2.52e-98 313
MS.gene000144.t1 MTR_4g063720 36.634 505 293 10 29 523 9 496 7.97e-97 308
MS.gene000144.t1 MTR_7g099680 36.170 517 279 11 29 523 9 496 1.46e-96 308
MS.gene000144.t1 MTR_4g063710 36.832 505 292 10 29 523 9 496 2.00e-96 307
MS.gene000144.t1 MTR_4g103780 40.570 456 151 7 118 569 1 340 3.07e-94 292
MS.gene000144.t1 MTR_2g038260 35.509 521 292 14 29 530 9 504 1.06e-87 284
MS.gene000144.t1 MTR_8g032650 35.271 516 302 14 28 531 8 503 5.97e-87 282
MS.gene000144.t1 MTR_2g437440 35.742 512 303 11 29 530 9 504 1.25e-86 280
MS.gene000144.t1 MTR_7g025770 34.308 513 308 12 29 530 9 503 1.42e-86 281
MS.gene000144.t1 MTR_7g451380 36.275 510 305 10 29 531 9 505 3.97e-86 280
MS.gene000144.t1 MTR_2g032550 35.897 507 295 13 29 523 10 498 1.32e-85 278
MS.gene000144.t1 MTR_7g026170 36.078 510 303 13 29 530 9 503 1.83e-85 278
MS.gene000144.t1 MTR_7g025620 33.704 540 328 13 29 557 9 529 4.86e-85 277
MS.gene000144.t1 MTR_8g099945 33.969 524 297 12 19 523 29 522 7.27e-85 278
MS.gene000144.t1 MTR_7g026090 33.640 544 323 14 29 554 9 532 2.08e-84 275
MS.gene000144.t1 MTR_8g099795 33.586 527 301 12 16 523 26 522 3.54e-84 276
MS.gene000144.t1 MTR_7g026110 34.291 522 316 13 28 539 8 512 1.56e-83 273
MS.gene000144.t1 MTR_7g025760 34.653 505 305 11 28 523 8 496 1.19e-82 271
MS.gene000144.t1 MTR_2g017730 32.943 513 307 11 29 523 8 501 1.33e-82 270
MS.gene000144.t1 MTR_7g029415 35.534 515 298 17 29 530 16 509 3.20e-82 270
MS.gene000144.t1 MTR_7g074460 33.658 514 304 12 28 535 8 490 4.86e-82 268
MS.gene000144.t1 MTR_7g021440 35.714 504 299 11 29 523 9 496 2.79e-81 267
MS.gene000144.t1 MTR_5g066800 35.135 518 294 15 29 523 9 507 2.79e-80 265
MS.gene000144.t1 MTR_7g025820 33.789 512 314 11 29 531 9 504 3.66e-80 263
MS.gene000144.t1 MTR_1g017130 33.792 509 276 15 29 530 9 463 9.54e-80 262
MS.gene000144.t1 MTR_2g022050 36.893 515 295 13 28 530 8 504 1.79e-79 262
MS.gene000144.t1 MTR_7g026130 35.465 516 297 17 29 529 9 503 2.77e-79 264
MS.gene000144.t1 MTR_7g026030 33.270 523 317 14 29 539 9 511 5.57e-79 261
MS.gene000144.t1 MTR_7g025720 33.465 508 306 11 28 523 8 495 7.78e-78 258
MS.gene000144.t1 MTR_7g025790 33.268 514 313 15 29 531 9 503 2.07e-77 256
MS.gene000144.t1 MTR_2g038270 32.946 516 320 13 30 531 10 513 2.87e-77 256
MS.gene000144.t1 MTR_7g026140 35.283 513 287 16 29 530 9 487 4.84e-77 256
MS.gene000144.t1 MTR_4g079590 33.202 506 309 13 29 523 9 496 6.07e-76 253
MS.gene000144.t1 MTR_6g033760 32.689 517 300 14 27 523 7 495 7.47e-76 253
MS.gene000144.t1 MTR_7g025630 34.357 521 302 14 28 539 8 497 1.22e-75 251
MS.gene000144.t1 MTR_6g033850 31.703 511 309 11 27 521 7 493 6.73e-75 251
MS.gene000144.t1 MTR_2g032580 33.333 516 300 13 28 532 8 490 2.26e-74 248
MS.gene000144.t1 MTR_7g025260 36.034 469 259 12 79 523 17 468 4.09e-74 248
MS.gene000144.t1 MTR_7g078210 35.078 516 302 17 29 531 9 504 4.62e-74 248
MS.gene000144.t1 MTR_4g027145 33.526 519 316 14 29 530 9 515 8.03e-74 248
MS.gene000144.t1 MTR_7g024030 33.074 514 314 14 29 531 9 503 9.05e-74 247
MS.gene000144.t1 MTR_7g011590 33.333 510 304 12 29 530 9 490 7.76e-73 244
MS.gene000144.t1 MTR_8g103930 32.588 537 284 16 27 530 9 500 5.18e-72 242
MS.gene000144.t1 MTR_7g078190 33.981 515 310 16 29 531 9 505 1.94e-71 241
MS.gene000144.t1 MTR_6g033955 32.079 505 320 12 27 521 7 498 3.24e-71 241
MS.gene000144.t1 MTR_2g010020 31.528 517 291 11 30 523 57 533 7.22e-71 241
MS.gene000144.t1 MTR_2g010020 31.528 517 291 11 30 523 58 534 7.51e-71 241
MS.gene000144.t1 MTR_6g033820 33.071 508 311 14 27 521 7 498 4.10e-70 239
MS.gene000144.t1 MTR_4g092920 29.616 547 313 12 5 523 29 531 4.93e-70 238
MS.gene000144.t1 MTR_7g025740 32.233 515 312 12 28 523 8 504 1.97e-69 236
MS.gene000144.t1 MTR_6g033870 32.567 522 296 16 27 521 8 500 4.18e-68 233
MS.gene000144.t1 MTR_7g451360 33.464 511 259 13 29 531 9 446 5.46e-67 228
MS.gene000144.t1 MTR_4g092920 32.824 393 233 7 141 523 26 397 3.18e-62 214
MS.gene000144.t1 MTR_7g007000 32.427 515 272 18 29 530 9 460 8.59e-62 214
MS.gene000144.t1 MTR_2g005690 32.157 510 305 16 27 523 69 550 9.23e-62 216
MS.gene000144.t1 MTR_6g033800 31.553 412 269 7 112 521 2 402 6.59e-60 209
MS.gene000144.t1 MTR_2g040160 33.753 397 233 12 29 414 1109 1486 9.52e-53 195
MS.gene000144.t1 MTR_2g032160 33.158 380 236 9 28 400 8 376 4.07e-52 185
MS.gene000144.t1 MTR_2g040370 32.836 402 224 10 29 411 1029 1403 1.77e-51 191
MS.gene000144.t1 MTR_2g040370 33.248 391 214 11 29 399 1413 1776 5.10e-50 187
MS.gene000144.t1 MTR_2g040370 32.836 402 224 10 29 411 1029 1403 1.93e-51 191
MS.gene000144.t1 MTR_2g040370 33.498 406 223 11 29 414 1413 1791 3.77e-50 187
MS.gene000144.t1 MTR_2g040220 32.398 392 239 11 30 410 1019 1395 5.44e-51 189
MS.gene000144.t1 MTR_2g040220 59.184 49 20 0 475 523 1399 1447 1.13e-11 68.6
MS.gene000144.t1 MTR_7g035155 30.545 514 272 18 29 530 9 449 4.28e-50 182
MS.gene000144.t1 MTR_4g063720 43.689 206 109 2 321 523 224 425 1.50e-48 178
MS.gene000144.t1 MTR_4g063720 32.287 223 141 4 29 250 9 222 4.77e-25 109
MS.gene000144.t1 MTR_2g040260 31.807 393 246 9 29 414 1038 1415 7.84e-48 180
MS.gene000144.t1 MTR_1g041565 29.175 497 266 15 29 521 5 419 1.02e-46 172
MS.gene000144.t1 MTR_2g040350 32.242 397 241 11 30 414 1039 1419 1.33e-46 176
MS.gene000144.t1 MTR_2g040350 32.242 397 241 11 30 414 821 1201 1.46e-46 176
MS.gene000144.t1 MTR_4g115870 42.000 250 76 7 92 338 57 240 1.87e-45 160
MS.gene000144.t1 MTR_4g115860 67.480 123 40 0 395 517 1 123 3.18e-45 156
MS.gene000144.t1 MTR_6g034005 29.384 422 273 10 104 521 4 404 8.35e-45 169
MS.gene000144.t1 MTR_7g026080 33.865 251 148 8 29 273 9 247 1.85e-35 134
MS.gene000144.t1 MTR_1g077230 31.944 288 173 9 29 307 9 282 1.01e-30 121
MS.gene000144.t1 MTR_7g026050 30.769 260 140 8 270 523 1 226 2.23e-28 116
MS.gene000144.t1 MTR_1g077240 36.216 185 113 3 348 531 3 183 1.15e-27 113
MS.gene000144.t1 MTR_4g012050 25.316 474 315 11 30 476 4 465 3.04e-27 117
MS.gene000144.t1 MTR_2g031670 33.878 245 141 9 41 275 43 276 4.20e-27 112
MS.gene000144.t1 MTR_4g115785 65.789 76 26 0 467 542 7 82 6.43e-27 104
MS.gene000144.t1 MTR_3g051300 25.799 438 291 13 30 455 4 419 4.27e-26 113
MS.gene000144.t1 MTR_6g033855 25.521 384 191 9 108 488 8 299 4.93e-26 109
MS.gene000144.t1 MTR_4g088515 30.573 314 177 10 29 333 276 557 5.64e-26 112
MS.gene000144.t1 MTR_4g088515 38.235 102 59 2 363 463 521 619 1.63e-11 67.4
MS.gene000144.t1 MTR_5g005870 32.995 197 124 3 329 523 75 265 6.44e-25 107
MS.gene000144.t1 MTR_2g102180 23.608 449 312 10 30 461 4 438 4.64e-23 104
MS.gene000144.t1 MTR_5g092570 24.719 445 301 13 30 459 4 429 3.43e-21 98.6
MS.gene000144.t1 MTR_6g033810 27.193 228 157 5 221 440 63 289 7.10e-19 87.8
MS.gene000144.t1 MTR_3g081170 22.846 499 313 15 23 479 20 488 4.54e-18 88.6
MS.gene000144.t1 MTR_7g074540 46.667 90 47 1 446 535 4 92 2.26e-16 78.2
MS.gene000144.t1 MTR_6g478100 30.097 206 115 7 90 293 39 217 2.61e-15 77.8
MS.gene000144.t1 MTR_1207s0010 32.168 143 89 2 134 275 7 142 4.52e-15 73.9
MS.gene000144.t1 MTR_5g097190 30.396 227 115 9 29 240 158 356 1.05e-13 73.2
MS.gene000144.t1 MTR_7g026180 34.959 123 78 2 98 219 23 144 1.02e-12 70.1
MS.gene000144.t1 MTR_7g451390 44.928 69 37 1 455 523 2 69 2.96e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000144.t1 AT2G32120 73.860 570 135 2 5 571 3 561 0.0 897
MS.gene000144.t1 AT2G32120 73.860 570 135 2 5 571 3 561 0.0 897
MS.gene000144.t1 AT3G12580 38.020 505 286 11 29 523 9 496 4.40e-102 322
MS.gene000144.t1 AT5G02500 37.030 505 291 10 29 523 9 496 2.03e-99 315
MS.gene000144.t1 AT3G09440 36.750 517 276 11 29 523 9 496 4.28e-98 312
MS.gene000144.t1 AT3G09440 36.750 517 276 11 29 523 9 496 4.28e-98 312
MS.gene000144.t1 AT3G09440 36.750 517 276 11 29 523 9 496 4.28e-98 312
MS.gene000144.t1 AT3G09440 36.750 517 276 11 29 523 9 496 4.28e-98 312
MS.gene000144.t1 AT1G16030 36.047 516 285 10 27 523 6 495 5.82e-98 311
MS.gene000144.t1 AT1G56410 37.030 505 291 10 29 523 9 496 7.05e-95 303
MS.gene000144.t1 AT5G02490 35.842 505 297 10 29 523 9 496 2.75e-94 302
MS.gene000144.t1 AT1G09080 34.469 499 306 8 30 523 43 525 6.63e-86 280
MS.gene000144.t1 AT1G09080 34.469 499 306 8 30 523 53 535 8.83e-86 280
MS.gene000144.t1 AT5G42020 33.268 514 320 10 16 523 25 521 2.45e-85 279
MS.gene000144.t1 AT5G42020 33.268 514 320 10 16 523 30 526 4.12e-85 278
MS.gene000144.t1 AT5G28540 33.800 500 309 9 30 523 38 521 3.95e-84 276
MS.gene000144.t1 AT5G42020 33.464 511 316 11 16 519 25 518 4.17e-84 274
MS.gene000144.t1 AT5G09590 30.843 522 305 10 22 523 52 537 3.18e-68 234
MS.gene000144.t1 AT4G37910 30.857 525 301 11 22 523 47 532 2.06e-64 223
MS.gene000144.t1 AT4G37910 30.857 525 301 11 22 523 47 532 2.06e-64 223
MS.gene000144.t1 AT5G02500 33.580 405 225 9 29 414 9 388 4.93e-63 216
MS.gene000144.t1 AT5G49910 31.299 508 312 14 27 523 78 559 9.39e-61 214
MS.gene000144.t1 AT4G24280 31.434 509 310 15 27 523 78 559 2.07e-59 210
MS.gene000144.t1 AT1G11660 26.464 461 305 16 30 476 4 444 2.45e-27 117
MS.gene000144.t1 AT1G11660 26.464 461 305 16 30 476 4 444 2.45e-27 117
MS.gene000144.t1 AT1G11660 26.464 461 305 16 30 476 4 444 2.45e-27 117
MS.gene000144.t1 AT1G79930 24.944 449 306 10 30 461 4 438 5.97e-26 113
MS.gene000144.t1 AT1G79930 24.944 449 306 10 30 461 4 438 6.14e-26 113
MS.gene000144.t1 AT1G11660 27.250 400 259 15 91 476 26 407 7.45e-24 106
MS.gene000144.t1 AT1G79920 24.499 449 308 10 30 461 4 438 1.01e-23 106
MS.gene000144.t1 AT1G79920 24.499 449 308 10 30 461 4 438 1.17e-23 106
MS.gene000144.t1 AT1G79920 24.499 449 308 10 30 461 4 438 1.17e-23 106
MS.gene000144.t1 AT1G79920 24.499 449 308 10 30 461 4 438 1.17e-23 106
MS.gene000144.t1 AT4G16660 24.722 360 238 10 143 480 164 512 1.46e-18 90.5
MS.gene000144.t1 AT4G16660 24.722 360 238 10 143 480 140 488 1.66e-18 90.1

Find 162 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGGATATTTCAAGATAA+TGG 0.182846 5.4:+1962151 MS.gene000144:CDS
TGGCACCATAGGTCCTATTT+AGG 0.200225 5.4:-1962352 None:intergenic
AGAAGTCCTTGCAATGTTTC+TGG 0.201625 5.4:+1961116 MS.gene000144:CDS
TCCATGCACACACTGATTTC+AGG 0.246998 5.4:-1962200 None:intergenic
TTCACTAACGCAGCAATAAA+TGG 0.261599 5.4:-1961066 None:intergenic
CAGTGATCCCTTTGGAAACT+TGG 0.262026 5.4:+1961932 MS.gene000144:CDS
TCAATTCCAATCGCTATTTC+AGG 0.266817 5.4:-1960763 None:intergenic
GTGGTGTTCCTTGGAATTAC+AGG 0.282170 5.4:-1962020 None:intergenic
CTGATTTCAGGAACTCCTTT+TGG 0.283375 5.4:-1962188 None:intergenic
TTCAAAGACGAAGCTCCTTC+TGG 0.283594 5.4:+1960886 MS.gene000144:CDS
GAAAGCTTCTCAAGAAACTA+TGG 0.285521 5.4:+1961329 MS.gene000144:CDS
TGTTCAGGTTGATGTAGATT+TGG 0.316072 5.4:+1961623 MS.gene000144:CDS
CTATGGTGCCACAATGGATT+TGG 0.318656 5.4:+1962364 MS.gene000144:CDS
AACATTGCAAGGACTTCTTC+TGG 0.325894 5.4:-1961111 None:intergenic
AAATGTGGTGCTTACTGTTC+CGG 0.327314 5.4:+1961185 MS.gene000144:CDS
TTAGGATATTTCAAGATAAT+GGG 0.332403 5.4:+1962152 MS.gene000144:CDS
ACAGCAGTGGCGTTATTATA+TGG 0.334823 5.4:+1961294 MS.gene000144:CDS
ATAGCGATTGGAATTGATAT+TGG 0.340874 5.4:+1960769 MS.gene000144:CDS
TAACAGGCACCACAGGTATA+CGG 0.347738 5.4:-1962277 None:intergenic
GGCACCATAGGTCCTATTTA+GGG 0.356331 5.4:-1962351 None:intergenic
TACCATCGGTGGAGAAGATT+TGG 0.359082 5.4:+1961464 MS.gene000144:CDS
GTTCAGGTTGATGTAGATTT+GGG 0.369664 5.4:+1961624 MS.gene000144:CDS
GGCATCCTCACTTCCATAAC+AGG 0.369992 5.4:-1962293 None:intergenic
AACGCCACTGCTGTTGGTTC+AGG 0.370548 5.4:-1961285 None:intergenic
ATCAACCTGAACACTGCTTT+GGG 0.377089 5.4:-1961613 None:intergenic
ATGGAAGGAGCTGTTGCTTC+AGG 0.378268 5.4:+1961906 MS.gene000144:CDS
CATCAACCTGAACACTGCTT+TGG 0.383070 5.4:-1961614 None:intergenic
TTCCACCCAAAGCAGTGTTC+AGG 0.388302 5.4:+1961608 MS.gene000144:CDS
GTAAGCACCACATTTCTTAT+CGG 0.392583 5.4:-1961177 None:intergenic
CACGACCATGAGATGCTGTT+TGG 0.393050 5.4:+1960931 MS.gene000144:CDS
TCGCACAAGCACGCTCTAAA+CGG 0.395103 5.4:-1961233 None:intergenic
TATACCTGTGGTGCCTGTTA+TGG 0.395756 5.4:+1962280 MS.gene000144:CDS
CATGTAAACATTGGATGATA+AGG 0.397163 5.4:-1961728 None:intergenic
TCCAAGGTCTGCACCAGAAA+TGG 0.397431 5.4:-1961030 None:intergenic
GCAGAAGAAAACCACTTGTT+AGG 0.403341 5.4:+1962134 MS.gene000144:CDS
TGGCTGGTCTTCATGTTCTC+AGG 0.414478 5.4:+1961256 MS.gene000144:CDS
TTATAAAGGTATAAACCCTT+TGG 0.418915 5.4:+1961860 MS.gene000144:CDS
AAAATAACAGAAGTTTATAA+AGG 0.432441 5.4:+1961846 MS.gene000144:CDS
CATGATGCAAAGGTAGAAGT+TGG 0.434870 5.4:+1961747 MS.gene000144:CDS
CCTTTGGAAGGTGCGGTTTG+TGG 0.435575 5.4:+1961876 MS.gene000144:CDS
CACGCTCTAAACGGGTCAGC+TGG 0.440361 5.4:-1961224 None:intergenic
GGTGTGCTGAGGCCCTAAAT+AGG 0.444919 5.4:+1962339 MS.gene000144:CDS
GCCATTTCTGGTGCAGACCT+TGG 0.447888 5.4:+1961029 MS.gene000144:CDS
ATGATGCAAAGGTAGAAGTT+GGG 0.448859 5.4:+1961748 MS.gene000144:CDS
GCTGGCATTGTGGTGTTCCT+TGG 0.449119 5.4:-1962029 None:intergenic
GCATCTGACAGTGAAACAAC+TGG 0.449771 5.4:+1960718 MS.gene000144:CDS
ATCAATTTATGCCTTCTTGA+GGG 0.450343 5.4:-1962390 None:intergenic
CTGCGTTAGTGAACAATGTC+TGG 0.455549 5.4:+1961076 MS.gene000144:CDS
TCAGGCATCAGTGATCCCTT+TGG 0.456587 5.4:+1961924 MS.gene000144:CDS
TGTTGACAGGGCGGAGTTTG+AGG 0.457797 5.4:+1961671 MS.gene000144:CDS
CTGGTGCAGACCTTGGACAT+TGG 0.457903 5.4:+1961036 MS.gene000144:CDS
GCCATTGGAATTCGAGCTGA+TGG 0.458770 5.4:+1961984 MS.gene000144:CDS
AAAATATATTCAAGCGTCAT+GGG 0.460640 5.4:+1961523 MS.gene000144:CDS
ATAAATGATGTGATACTTGT+TGG 0.460986 5.4:+1961774 MS.gene000144:CDS
CGCACAAGCACGCTCTAAAC+GGG 0.463935 5.4:-1961232 None:intergenic
GTAACAAGATTCTCCACCCT+TGG 0.466538 5.4:-1961813 None:intergenic
GAGCGTGCTTGTGCGATGGC+TGG 0.467592 5.4:+1961240 MS.gene000144:CDS
TTAGCCGGTGTTGTGATGCC+TGG 0.471501 5.4:+1962251 MS.gene000144:CDS
TGGAAACGACTGAATGAAAC+CGG 0.482736 5.4:-1961204 None:intergenic
CAAGCCACTCCTCTTGCCAT+TGG 0.482744 5.4:+1961969 MS.gene000144:CDS
CCAATCGCTATTTCAGGAAG+AGG 0.484412 5.4:-1960757 None:intergenic
GTTGATGTAGATTTGGGAGA+TGG 0.485617 5.4:+1961630 MS.gene000144:CDS
TATAATAACGCCACTGCTGT+TGG 0.489694 5.4:-1961291 None:intergenic
GGTCAAAGAAATTAAATCAA+TGG 0.491607 5.4:+1961545 MS.gene000144:CDS
TAACAAGATTCTCCACCCTT+GGG 0.492275 5.4:-1961812 None:intergenic
GAAAATATATTCAAGCGTCA+TGG 0.496398 5.4:+1961522 MS.gene000144:CDS
CAAATCCATTGTGGCACCAT+AGG 0.497810 5.4:-1962363 None:intergenic
AAAGCTGATGAATTGCATCC+TGG 0.503417 5.4:-1961587 None:intergenic
CCTCTTCCTGAAATAGCGAT+TGG 0.506297 5.4:+1960757 MS.gene000144:CDS
GTTGCTGTGACTGCTACCGC+CGG 0.506470 5.4:+1961408 MS.gene000144:CDS
TTGCTGTGACTGCTACCGCC+GGG 0.511136 5.4:+1961409 MS.gene000144:CDS
AACAAGTCCAAGTTTCCAAA+GGG 0.511681 5.4:-1961939 None:intergenic
AGATATGCAAGGTTGTTGAC+AGG 0.512232 5.4:+1961658 MS.gene000144:CDS
TATCAATTTATGCCTTCTTG+AGG 0.516603 5.4:-1962391 None:intergenic
AACAGGCACCACAGGTATAC+GGG 0.517047 5.4:-1962276 None:intergenic
GGAATACCTGCAGCACCAAA+AGG 0.524506 5.4:+1962173 MS.gene000144:CDS
CAAACAGCATCTCATGGTCG+TGG 0.528773 5.4:-1960930 None:intergenic
CACAAACCGCACCTTCCAAA+GGG 0.529952 5.4:-1961875 None:intergenic
TTCAATATGAAACGCTTGAT+CGG 0.530493 5.4:+1960964 MS.gene000144:CDS
CCTTTGCATCATGTAAACAT+TGG 0.531721 5.4:-1961737 None:intergenic
GAGGCCCTAAATAGGACCTA+TGG 0.533257 5.4:+1962347 MS.gene000144:CDS
TGCGGTTTGTGGTGCAGCAA+TGG 0.533347 5.4:+1961887 MS.gene000144:CDS
ACCACAGGTATACGGGATCC+AGG 0.535468 5.4:-1962269 None:intergenic
TCCTGAAATCAGTGTGTGCA+TGG 0.540172 5.4:+1962199 MS.gene000144:CDS
TCTTGAAATATCCTAACAAG+TGG 0.545629 5.4:-1962145 None:intergenic
AAAAGTAGATCTTTACGTGC+TGG 0.548488 5.4:-1962047 None:intergenic
AACCTGAACACTGCTTTGGG+TGG 0.551609 5.4:-1961610 None:intergenic
AAGGACTTCTTCTGGAGTGG+TGG 0.559811 5.4:-1961103 None:intergenic
TGCAAGGACTTCTTCTGGAG+TGG 0.559852 5.4:-1961106 None:intergenic
TCCCAGATAAAAGCATTGGC+AGG 0.560848 5.4:+1961438 MS.gene000144:CDS
TCTCAAGAAACTATGGGCAG+TGG 0.563843 5.4:+1961336 MS.gene000144:CDS
GGATTTGGTTACCCTCAAGA+AGG 0.565408 5.4:+1962379 MS.gene000144:CDS
AACCAAATCTTCTCCACCGA+TGG 0.568389 5.4:-1961466 None:intergenic
TGGACGAACGCCAATGTCCA+AGG 0.570950 5.4:-1961046 None:intergenic
GTACGAAGGTGAGGGAAAGA+AGG 0.571023 5.4:+1962112 MS.gene000144:CDS
TTTAGAGCGTGCTTGTGCGA+TGG 0.572607 5.4:+1961236 MS.gene000144:CDS
TCAGATGCAACGGTATATGC+AGG 0.578112 5.4:-1960703 None:intergenic
GATACTTGTGTACGAAGGTG+AGG 0.578994 5.4:+1962103 MS.gene000144:CDS
AAAGGTATAAACCCTTTGGA+AGG 0.581870 5.4:+1961864 MS.gene000144:CDS
TGTTTCACTGTCAGATGCAA+CGG 0.582470 5.4:-1960713 None:intergenic
CCCATCAGCTCGAATTCCAA+TGG 0.583989 5.4:-1961985 None:intergenic
TAACAAGTCCAAGTTTCCAA+AGG 0.584586 5.4:-1961940 None:intergenic
GAGGATGTTCTTATATCCCA+AGG 0.584781 5.4:+1961796 MS.gene000144:CDS
CCATGACCATCATCAACCAT+TGG 0.585720 5.4:-1962314 None:intergenic
AGTCCTTGCAATGTTTCTGG+TGG 0.586924 5.4:+1961119 MS.gene000144:CDS
AAGCTGATGAATTGCATCCT+GGG 0.587754 5.4:-1961586 None:intergenic
AGTATCCCAGATAAAAGCAT+TGG 0.589832 5.4:+1961434 MS.gene000144:CDS
ATTGCTTCGAGTTGCAACCC+AGG 0.590200 5.4:+1961569 MS.gene000144:CDS
CCACAAACCGCACCTTCCAA+AGG 0.591096 5.4:-1961876 None:intergenic
TGTAGTGTTGCTGTGTGGAA+TGG 0.591608 5.4:+1960802 MS.gene000144:CDS
TGATGCAAAGGTAGAAGTTG+GGG 0.591780 5.4:+1961749 MS.gene000144:CDS
ACTTCCATAACAGGCACCAC+AGG 0.592348 5.4:-1962284 None:intergenic
CCAATGTTTACATGATGCAA+AGG 0.592560 5.4:+1961737 MS.gene000144:CDS
TGCTGTGACTGCTACCGCCG+GGG 0.595836 5.4:+1961410 MS.gene000144:CDS
CACAGCAACACTACATTGTG+AGG 0.598044 5.4:-1960794 None:intergenic
CATCTGACAGTGAAACAACT+GGG 0.598563 5.4:+1960719 MS.gene000144:CDS
ATGGTCGTGGGAGAATTGAC+TGG 0.601519 5.4:-1960917 None:intergenic
CCATTGGAATTCGAGCTGAT+GGG 0.601553 5.4:+1961985 MS.gene000144:CDS
AGTTTGTACCTGTAATTCCA+AGG 0.609145 5.4:+1962012 MS.gene000144:CDS
AAAGACGAAGCTCCTTCTGG+AGG 0.611357 5.4:+1960889 MS.gene000144:CDS
ATCTTTACGTGCTGGCATTG+TGG 0.613475 5.4:-1962039 None:intergenic
AATTCCAATGGCAAGAGGAG+TGG 0.613750 5.4:-1961973 None:intergenic
AAACAGCATCTCATGGTCGT+GGG 0.616728 5.4:-1960929 None:intergenic
TATCTCCAAACAGCATCTCA+TGG 0.617418 5.4:-1960936 None:intergenic
GTTTGTGGTGCAGCAATGGA+AGG 0.619096 5.4:+1961891 MS.gene000144:CDS
TGGTCATGGTTGGTGTGCTG+AGG 0.620833 5.4:+1962328 MS.gene000144:CDS
AAAGCTTCTCAAGAAACTAT+GGG 0.624225 5.4:+1961330 MS.gene000144:CDS
AATGATGTGATACTTGTTGG+AGG 0.629718 5.4:+1961777 MS.gene000144:CDS
TAGGACCTATGGTGCCACAA+TGG 0.630018 5.4:+1962358 MS.gene000144:CDS
TGGTGCCTGTTATGGAAGTG+AGG 0.631903 5.4:+1962288 MS.gene000144:CDS
TGACAGGGCGGAGTTTGAGG+AGG 0.633647 5.4:+1961674 MS.gene000144:CDS
TATAAACCCTTTGGAAGGTG+CGG 0.634750 5.4:+1961869 MS.gene000144:CDS
GCATTGGCAGGAAGTACCAT+CGG 0.637875 5.4:+1961450 MS.gene000144:CDS
AGGATGCCAATGGTTGATGA+TGG 0.639984 5.4:+1962308 MS.gene000144:CDS
GGATCCAGGCATCACAACAC+CGG 0.644727 5.4:-1962255 None:intergenic
GATATGCAAGGTTGTTGACA+GGG 0.644837 5.4:+1961659 MS.gene000144:CDS
ATACTTGTGTACGAAGGTGA+GGG 0.646011 5.4:+1962104 MS.gene000144:CDS
AGGATGTTCTTATATCCCAA+GGG 0.646556 5.4:+1961797 MS.gene000144:CDS
GCTCGAATTCCAATGGCAAG+AGG 0.649760 5.4:-1961978 None:intergenic
TGGTTGATGATGGTCATGGT+TGG 0.651359 5.4:+1962318 MS.gene000144:CDS
CAAACGACCGATAAGAAATG+TGG 0.653357 5.4:+1961170 MS.gene000144:CDS
TGACAGTGAAACAACTGGGG+AGG 0.653527 5.4:+1960723 MS.gene000144:CDS
ACTCCACCAGAAACATTGCA+AGG 0.653765 5.4:-1961122 None:intergenic
GCCTGGATCCCGTATACCTG+TGG 0.656774 5.4:+1962268 MS.gene000144:CDS
GTTTGAGGAGGTGAACAATG+AGG 0.660540 5.4:+1961686 MS.gene000144:CDS
CCAATGGTTGATGATGGTCA+TGG 0.660629 5.4:+1962314 MS.gene000144:CDS
ATGTTCTTATATCCCAAGGG+TGG 0.662483 5.4:+1961800 MS.gene000144:CDS
TGACTGGTAACTCCTCCAGA+AGG 0.672347 5.4:-1960901 None:intergenic
GAGGGTAACCAAATCCATTG+TGG 0.675212 5.4:-1962372 None:intergenic
GATGCCTGAACCAACAGCAG+TGG 0.675835 5.4:+1961281 MS.gene000144:CDS
CAGAGTTGATACTGACCCTG+TGG 0.678557 5.4:+1960987 MS.gene000144:CDS
TATTCATGATAATCAAACTG+AGG 0.679559 5.4:+1962076 MS.gene000144:CDS
GCTGTGACTGCTACCGCCGG+GGG 0.681642 5.4:+1961411 MS.gene000144:CDS
AAATATATTCAAGCGTCATG+GGG 0.682975 5.4:+1961524 MS.gene000144:CDS
CACAATGTAGTGTTGCTGTG+TGG 0.686328 5.4:+1960797 MS.gene000144:CDS
AGATGGTTTGAAGATATGCA+AGG 0.687926 5.4:+1961647 MS.gene000144:CDS
TTGGCAGGAAGTACCATCGG+TGG 0.690515 5.4:+1961453 MS.gene000144:CDS
ATGCAAGGTTGTTGACAGGG+CGG 0.708122 5.4:+1961662 MS.gene000144:CDS
TGTGTGGAATGGCTCCGAAG+TGG 0.718800 5.4:+1960813 MS.gene000144:CDS
TATGGAAGTGAGGATGCCAA+TGG 0.723950 5.4:+1962298 MS.gene000144:CDS
ATCTGACAGTGAAACAACTG+GGG 0.741112 5.4:+1960720 MS.gene000144:CDS
GCATTGATACTTGTGTACGA+AGG 0.792141 5.4:+1962098 MS.gene000144:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAACAGAAGTTTATAA+AGG + chr5.4:1961846-1961865 MS.gene000144:CDS 15.0%
!! TTAGGATATTTCAAGATAAT+GGG + chr5.4:1962152-1962171 MS.gene000144:CDS 20.0%
!!! TTGAATATATTTTCAGAATC+TGG - chr5.4:1961516-1961535 None:intergenic 20.0%
! AAAATATATTCAAGCGTCAT+GGG + chr5.4:1961523-1961542 MS.gene000144:CDS 25.0%
! ATAAATGATGTGATACTTGT+TGG + chr5.4:1961774-1961793 MS.gene000144:CDS 25.0%
! GGTCAAAGAAATTAAATCAA+TGG + chr5.4:1961545-1961564 MS.gene000144:CDS 25.0%
! GTTAGGATATTTCAAGATAA+TGG + chr5.4:1962151-1962170 MS.gene000144:CDS 25.0%
! TATTCATGATAATCAAACTG+AGG + chr5.4:1962076-1962095 MS.gene000144:CDS 25.0%
!! AATTGCTCTCATTTTCAATA+TGG + chr5.4:1961368-1961387 MS.gene000144:CDS 25.0%
!! ATTGCTCTCATTTTCAATAT+GGG + chr5.4:1961369-1961388 MS.gene000144:CDS 25.0%
!! TTATAAAGGTATAAACCCTT+TGG + chr5.4:1961860-1961879 MS.gene000144:CDS 25.0%
!!! AACTTCTGTTATTTTGCATA+AGG - chr5.4:1961841-1961860 None:intergenic 25.0%
AAAGCTTCTCAAGAAACTAT+GGG + chr5.4:1961330-1961349 MS.gene000144:CDS 30.0%
AAATATATTCAAGCGTCATG+GGG + chr5.4:1961524-1961543 MS.gene000144:CDS 30.0%
ATAGCGATTGGAATTGATAT+TGG + chr5.4:1960769-1960788 MS.gene000144:CDS 30.0%
CATGTAAACATTGGATGATA+AGG - chr5.4:1961731-1961750 None:intergenic 30.0%
GAAAATATATTCAAGCGTCA+TGG + chr5.4:1961522-1961541 MS.gene000144:CDS 30.0%
TCTTGAAATATCCTAACAAG+TGG - chr5.4:1962148-1962167 None:intergenic 30.0%
TTCAATATGAAACGCTTGAT+CGG + chr5.4:1960964-1960983 MS.gene000144:CDS 30.0%
! AAGTAAAAATCTGCCATTTC+TGG + chr5.4:1961017-1961036 MS.gene000144:CDS 30.0%
AAAAGTAGATCTTTACGTGC+TGG - chr5.4:1962050-1962069 None:intergenic 35.0%
AACAAGTCCAAGTTTCCAAA+GGG - chr5.4:1961942-1961961 None:intergenic 35.0%
AGATGGTTTGAAGATATGCA+AGG + chr5.4:1961647-1961666 MS.gene000144:CDS 35.0%
AGGATGTTCTTATATCCCAA+GGG + chr5.4:1961797-1961816 MS.gene000144:CDS 35.0%
AGTTTGTACCTGTAATTCCA+AGG + chr5.4:1962012-1962031 MS.gene000144:CDS 35.0%
ATGATGCAAAGGTAGAAGTT+GGG + chr5.4:1961748-1961767 MS.gene000144:CDS 35.0%
CCAATGTTTACATGATGCAA+AGG + chr5.4:1961737-1961756 MS.gene000144:CDS 35.0%
CCTTTGCATCATGTAAACAT+TGG - chr5.4:1961740-1961759 None:intergenic 35.0%
GAAAGCTTCTCAAGAAACTA+TGG + chr5.4:1961329-1961348 MS.gene000144:CDS 35.0%
GTAAGCACCACATTTCTTAT+CGG - chr5.4:1961180-1961199 None:intergenic 35.0%
TAACAAGTCCAAGTTTCCAA+AGG - chr5.4:1961943-1961962 None:intergenic 35.0%
TTCACTAACGCAGCAATAAA+TGG - chr5.4:1961069-1961088 None:intergenic 35.0%
! AAAGGTATAAACCCTTTGGA+AGG + chr5.4:1961864-1961883 MS.gene000144:CDS 35.0%
! AATGATGTGATACTTGTTGG+AGG + chr5.4:1961777-1961796 MS.gene000144:CDS 35.0%
! AGTATCCCAGATAAAAGCAT+TGG + chr5.4:1961434-1961453 MS.gene000144:CDS 35.0%
! TCAATTCCAATCGCTATTTC+AGG - chr5.4:1960766-1960785 None:intergenic 35.0%
!! GTTCAGGTTGATGTAGATTT+GGG + chr5.4:1961624-1961643 MS.gene000144:CDS 35.0%
!! GTTTTTCAAAAGCTCCACTT+CGG - chr5.4:1960830-1960849 None:intergenic 35.0%
!! TGTTCAGGTTGATGTAGATT+TGG + chr5.4:1961623-1961642 MS.gene000144:CDS 35.0%
AAAGCTGATGAATTGCATCC+TGG - chr5.4:1961590-1961609 None:intergenic 40.0%
AAATGTGGTGCTTACTGTTC+CGG + chr5.4:1961185-1961204 MS.gene000144:CDS 40.0%
AACATTGCAAGGACTTCTTC+TGG - chr5.4:1961114-1961133 None:intergenic 40.0%
AAGCTGATGAATTGCATCCT+GGG - chr5.4:1961589-1961608 None:intergenic 40.0%
ACAGCAGTGGCGTTATTATA+TGG + chr5.4:1961294-1961313 MS.gene000144:CDS 40.0%
AGAAGTCCTTGCAATGTTTC+TGG + chr5.4:1961116-1961135 MS.gene000144:CDS 40.0%
AGATATGCAAGGTTGTTGAC+AGG + chr5.4:1961658-1961677 MS.gene000144:CDS 40.0%
ATACTTGTGTACGAAGGTGA+GGG + chr5.4:1962104-1962123 MS.gene000144:CDS 40.0%
ATCAACCTGAACACTGCTTT+GGG - chr5.4:1961616-1961635 None:intergenic 40.0%
ATCTGACAGTGAAACAACTG+GGG + chr5.4:1960720-1960739 MS.gene000144:CDS 40.0%
ATGTTCTTATATCCCAAGGG+TGG + chr5.4:1961800-1961819 MS.gene000144:CDS 40.0%
CAAACGACCGATAAGAAATG+TGG + chr5.4:1961170-1961189 MS.gene000144:CDS 40.0%
CATCTGACAGTGAAACAACT+GGG + chr5.4:1960719-1960738 MS.gene000144:CDS 40.0%
CATGATGCAAAGGTAGAAGT+TGG + chr5.4:1961747-1961766 MS.gene000144:CDS 40.0%
GAGGATGTTCTTATATCCCA+AGG + chr5.4:1961796-1961815 MS.gene000144:CDS 40.0%
GATATGCAAGGTTGTTGACA+GGG + chr5.4:1961659-1961678 MS.gene000144:CDS 40.0%
GCAGAAGAAAACCACTTGTT+AGG + chr5.4:1962134-1962153 MS.gene000144:CDS 40.0%
GTTGATGTAGATTTGGGAGA+TGG + chr5.4:1961630-1961649 MS.gene000144:CDS 40.0%
TAACAAGATTCTCCACCCTT+GGG - chr5.4:1961815-1961834 None:intergenic 40.0%
TATAATAACGCCACTGCTGT+TGG - chr5.4:1961294-1961313 None:intergenic 40.0%
TATCTCCAAACAGCATCTCA+TGG - chr5.4:1960939-1960958 None:intergenic 40.0%
TGATGCAAAGGTAGAAGTTG+GGG + chr5.4:1961749-1961768 MS.gene000144:CDS 40.0%
TGGAAACGACTGAATGAAAC+CGG - chr5.4:1961207-1961226 None:intergenic 40.0%
TGTTTCACTGTCAGATGCAA+CGG - chr5.4:1960716-1960735 None:intergenic 40.0%
! CTCATTTTCAATATGGGTGC+TGG + chr5.4:1961375-1961394 MS.gene000144:CDS 40.0%
! CTGATTTCAGGAACTCCTTT+TGG - chr5.4:1962191-1962210 None:intergenic 40.0%
! GCATTGATACTTGTGTACGA+AGG + chr5.4:1962098-1962117 MS.gene000144:CDS 40.0%
! TATAAACCCTTTGGAAGGTG+CGG + chr5.4:1961869-1961888 MS.gene000144:CDS 40.0%
! TCCTGCCAATGCTTTTATCT+GGG - chr5.4:1961442-1961461 None:intergenic 40.0%
! TTCCTGCCAATGCTTTTATC+TGG - chr5.4:1961443-1961462 None:intergenic 40.0%
! TTTTACTTGCGTGAACCACA+GGG - chr5.4:1961005-1961024 None:intergenic 40.0%
! TTTTTACTTGCGTGAACCAC+AGG - chr5.4:1961006-1961025 None:intergenic 40.0%
AAACAGCATCTCATGGTCGT+GGG - chr5.4:1960932-1960951 None:intergenic 45.0%
AACCAAATCTTCTCCACCGA+TGG - chr5.4:1961469-1961488 None:intergenic 45.0%
AATTCCAATGGCAAGAGGAG+TGG - chr5.4:1961976-1961995 None:intergenic 45.0%
ACTCCACCAGAAACATTGCA+AGG - chr5.4:1961125-1961144 None:intergenic 45.0%
CAAATCCATTGTGGCACCAT+AGG - chr5.4:1962366-1962385 None:intergenic 45.0%
CACAGCAACACTACATTGTG+AGG - chr5.4:1960797-1960816 None:intergenic 45.0%
CATCAACCTGAACACTGCTT+TGG - chr5.4:1961617-1961636 None:intergenic 45.0%
CCATGACCATCATCAACCAT+TGG - chr5.4:1962317-1962336 None:intergenic 45.0%
CCATTGGAATTCGAGCTGAT+GGG + chr5.4:1961985-1962004 MS.gene000144:CDS 45.0%
CCTCTTCCTGAAATAGCGAT+TGG + chr5.4:1960757-1960776 MS.gene000144:CDS 45.0%
CTGCGTTAGTGAACAATGTC+TGG + chr5.4:1961076-1961095 MS.gene000144:CDS 45.0%
GAGGGTAACCAAATCCATTG+TGG - chr5.4:1962375-1962394 None:intergenic 45.0%
GATACTTGTGTACGAAGGTG+AGG + chr5.4:1962103-1962122 MS.gene000144:CDS 45.0%
GCATCTGACAGTGAAACAAC+TGG + chr5.4:1960718-1960737 MS.gene000144:CDS 45.0%
GGCACCATAGGTCCTATTTA+GGG - chr5.4:1962354-1962373 None:intergenic 45.0%
GTAACAAGATTCTCCACCCT+TGG - chr5.4:1961816-1961835 None:intergenic 45.0%
GTGGTGTTCCTTGGAATTAC+AGG - chr5.4:1962023-1962042 None:intergenic 45.0%
GTTTGAGGAGGTGAACAATG+AGG + chr5.4:1961686-1961705 MS.gene000144:CDS 45.0%
TAACAGGCACCACAGGTATA+CGG - chr5.4:1962280-1962299 None:intergenic 45.0%
TACCATCGGTGGAGAAGATT+TGG + chr5.4:1961464-1961483 MS.gene000144:CDS 45.0%
TATACCTGTGGTGCCTGTTA+TGG + chr5.4:1962280-1962299 MS.gene000144:CDS 45.0%
TATGGAAGTGAGGATGCCAA+TGG + chr5.4:1962298-1962317 MS.gene000144:CDS 45.0%
TCAGATGCAACGGTATATGC+AGG - chr5.4:1960706-1960725 None:intergenic 45.0%
TCCTGAAATCAGTGTGTGCA+TGG + chr5.4:1962199-1962218 MS.gene000144:CDS 45.0%
TCTCAAGAAACTATGGGCAG+TGG + chr5.4:1961336-1961355 MS.gene000144:CDS 45.0%
TGGCACCATAGGTCCTATTT+AGG - chr5.4:1962355-1962374 None:intergenic 45.0%
TTCAAAGACGAAGCTCCTTC+TGG + chr5.4:1960886-1960905 MS.gene000144:CDS 45.0%
! ATCTTTACGTGCTGGCATTG+TGG - chr5.4:1962042-1962061 None:intergenic 45.0%
! CACAATGTAGTGTTGCTGTG+TGG + chr5.4:1960797-1960816 MS.gene000144:CDS 45.0%
! CAGTGATCCCTTTGGAAACT+TGG + chr5.4:1961932-1961951 MS.gene000144:CDS 45.0%
! CCAATCGCTATTTCAGGAAG+AGG - chr5.4:1960760-1960779 None:intergenic 45.0%
! GGATTTGGTTACCCTCAAGA+AGG + chr5.4:1962379-1962398 MS.gene000144:CDS 45.0%
! TGTAGTGTTGCTGTGTGGAA+TGG + chr5.4:1960802-1960821 MS.gene000144:CDS 45.0%
!! AGGATGCCAATGGTTGATGA+TGG + chr5.4:1962308-1962327 MS.gene000144:CDS 45.0%
!! AGTCCTTGCAATGTTTCTGG+TGG + chr5.4:1961119-1961138 MS.gene000144:CDS 45.0%
!! CCAATGGTTGATGATGGTCA+TGG + chr5.4:1962314-1962333 MS.gene000144:CDS 45.0%
!! CTATGGTGCCACAATGGATT+TGG + chr5.4:1962364-1962383 MS.gene000144:CDS 45.0%
!! TCCATGCACACACTGATTTC+AGG - chr5.4:1962203-1962222 None:intergenic 45.0%
!! TCCCAGATAAAAGCATTGGC+AGG + chr5.4:1961438-1961457 MS.gene000144:CDS 45.0%
!! TGGTTGATGATGGTCATGGT+TGG + chr5.4:1962318-1962337 MS.gene000144:CDS 45.0%
!! TTTTATCTGGGATACTCCCC+CGG - chr5.4:1961430-1961449 None:intergenic 45.0%
AAAGACGAAGCTCCTTCTGG+AGG + chr5.4:1960889-1960908 MS.gene000144:CDS 50.0%
AACAGGCACCACAGGTATAC+GGG - chr5.4:1962279-1962298 None:intergenic 50.0%
ACTTCCATAACAGGCACCAC+AGG - chr5.4:1962287-1962306 None:intergenic 50.0%
ATGCAAGGTTGTTGACAGGG+CGG + chr5.4:1961662-1961681 MS.gene000144:CDS 50.0%
ATGGTCGTGGGAGAATTGAC+TGG - chr5.4:1960920-1960939 None:intergenic 50.0%
CAAACAGCATCTCATGGTCG+TGG - chr5.4:1960933-1960952 None:intergenic 50.0%
CACAAACCGCACCTTCCAAA+GGG - chr5.4:1961878-1961897 None:intergenic 50.0%
CACGACCATGAGATGCTGTT+TGG + chr5.4:1960931-1960950 MS.gene000144:CDS 50.0%
CCCATCAGCTCGAATTCCAA+TGG - chr5.4:1961988-1962007 None:intergenic 50.0%
GAGGCCCTAAATAGGACCTA+TGG + chr5.4:1962347-1962366 MS.gene000144:CDS 50.0%
GCCATTGGAATTCGAGCTGA+TGG + chr5.4:1961984-1962003 MS.gene000144:CDS 50.0%
GCTCGAATTCCAATGGCAAG+AGG - chr5.4:1961981-1962000 None:intergenic 50.0%
GGAATACCTGCAGCACCAAA+AGG + chr5.4:1962173-1962192 MS.gene000144:CDS 50.0%
GGCATCCTCACTTCCATAAC+AGG - chr5.4:1962296-1962315 None:intergenic 50.0%
GTACGAAGGTGAGGGAAAGA+AGG + chr5.4:1962112-1962131 MS.gene000144:CDS 50.0%
GTTTGTGGTGCAGCAATGGA+AGG + chr5.4:1961891-1961910 MS.gene000144:CDS 50.0%
TCAGGCATCAGTGATCCCTT+TGG + chr5.4:1961924-1961943 MS.gene000144:CDS 50.0%
TCCAAGGTCTGCACCAGAAA+TGG - chr5.4:1961033-1961052 None:intergenic 50.0%
TGACAGTGAAACAACTGGGG+AGG + chr5.4:1960723-1960742 MS.gene000144:CDS 50.0%
TGACTGGTAACTCCTCCAGA+AGG - chr5.4:1960904-1960923 None:intergenic 50.0%
TGGTGCCTGTTATGGAAGTG+AGG + chr5.4:1962288-1962307 MS.gene000144:CDS 50.0%
TTCCACCCAAAGCAGTGTTC+AGG + chr5.4:1961608-1961627 MS.gene000144:CDS 50.0%
! AACCTGAACACTGCTTTGGG+TGG - chr5.4:1961613-1961632 None:intergenic 50.0%
! ATGGAAGGAGCTGTTGCTTC+AGG + chr5.4:1961906-1961925 MS.gene000144:CDS 50.0%
! TCGCACAAGCACGCTCTAAA+CGG - chr5.4:1961236-1961255 None:intergenic 50.0%
! TGCAAGGACTTCTTCTGGAG+TGG - chr5.4:1961109-1961128 None:intergenic 50.0%
! TTTAGAGCGTGCTTGTGCGA+TGG + chr5.4:1961236-1961255 MS.gene000144:CDS 50.0%
!! AACGTGCTGCGAGTTTTAGC+CGG + chr5.4:1962236-1962255 MS.gene000144:CDS 50.0%
!! AAGGACTTCTTCTGGAGTGG+TGG - chr5.4:1961106-1961125 None:intergenic 50.0%
!! ATTGCTTCGAGTTGCAACCC+AGG + chr5.4:1961569-1961588 MS.gene000144:CDS 50.0%
!! CAGAGTTGATACTGACCCTG+TGG + chr5.4:1960987-1961006 MS.gene000144:CDS 50.0%
!! GCATTGGCAGGAAGTACCAT+CGG + chr5.4:1961450-1961469 MS.gene000144:CDS 50.0%
!! TAGGACCTATGGTGCCACAA+TGG + chr5.4:1962358-1962377 MS.gene000144:CDS 50.0%
!! TGGCTGGTCTTCATGTTCTC+AGG + chr5.4:1961256-1961275 MS.gene000144:CDS 50.0%
ACCACAGGTATACGGGATCC+AGG - chr5.4:1962272-1962291 None:intergenic 55.0%
CAAGCCACTCCTCTTGCCAT+TGG + chr5.4:1961969-1961988 MS.gene000144:CDS 55.0%
CCACAAACCGCACCTTCCAA+AGG - chr5.4:1961879-1961898 None:intergenic 55.0%
CTGGTGCAGACCTTGGACAT+TGG + chr5.4:1961036-1961055 MS.gene000144:CDS 55.0%
GATGCCTGAACCAACAGCAG+TGG + chr5.4:1961281-1961300 MS.gene000144:CDS 55.0%
GCTGGCATTGTGGTGTTCCT+TGG - chr5.4:1962032-1962051 None:intergenic 55.0%
GGATCCAGGCATCACAACAC+CGG - chr5.4:1962258-1962277 None:intergenic 55.0%
GGTGTGCTGAGGCCCTAAAT+AGG + chr5.4:1962339-1962358 MS.gene000144:CDS 55.0%
TGCGGTTTGTGGTGCAGCAA+TGG + chr5.4:1961887-1961906 MS.gene000144:CDS 55.0%
TGGACGAACGCCAATGTCCA+AGG - chr5.4:1961049-1961068 None:intergenic 55.0%
TGTGTGGAATGGCTCCGAAG+TGG + chr5.4:1960813-1960832 MS.gene000144:CDS 55.0%
TGTTGACAGGGCGGAGTTTG+AGG + chr5.4:1961671-1961690 MS.gene000144:CDS 55.0%
! CCTTTGGAAGGTGCGGTTTG+TGG + chr5.4:1961876-1961895 MS.gene000144:CDS 55.0%
! CGCACAAGCACGCTCTAAAC+GGG - chr5.4:1961235-1961254 None:intergenic 55.0%
! TGGTCATGGTTGGTGTGCTG+AGG + chr5.4:1962328-1962347 MS.gene000144:CDS 55.0%
!! AACGCCACTGCTGTTGGTTC+AGG - chr5.4:1961288-1961307 None:intergenic 55.0%
!! GCCATTTCTGGTGCAGACCT+TGG + chr5.4:1961029-1961048 MS.gene000144:CDS 55.0%
!! TTAGCCGGTGTTGTGATGCC+TGG + chr5.4:1962251-1962270 MS.gene000144:CDS 55.0%
!! TTGGCAGGAAGTACCATCGG+TGG + chr5.4:1961453-1961472 MS.gene000144:CDS 55.0%
!!! GGAACTCCTTTTGGTGCTGC+AGG - chr5.4:1962182-1962201 None:intergenic 55.0%
GCCTGGATCCCGTATACCTG+TGG + chr5.4:1962268-1962287 MS.gene000144:CDS 60.0%
GTTGCTGTGACTGCTACCGC+CGG + chr5.4:1961408-1961427 MS.gene000144:CDS 60.0%
TGACAGGGCGGAGTTTGAGG+AGG + chr5.4:1961674-1961693 MS.gene000144:CDS 60.0%
TTGCTGTGACTGCTACCGCC+GGG + chr5.4:1961409-1961428 MS.gene000144:CDS 60.0%
! CACGCTCTAAACGGGTCAGC+TGG - chr5.4:1961227-1961246 None:intergenic 60.0%
! TATCTGGGATACTCCCCCGG+CGG - chr5.4:1961427-1961446 None:intergenic 60.0%
GAGCGTGCTTGTGCGATGGC+TGG + chr5.4:1961240-1961259 MS.gene000144:CDS 65.0%
TGCTGTGACTGCTACCGCCG+GGG + chr5.4:1961410-1961429 MS.gene000144:CDS 65.0%
GCTGTGACTGCTACCGCCGG+GGG + chr5.4:1961411-1961430 MS.gene000144:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 1960688 1962406 1960688 ID=MS.gene000144
chr5.4 mRNA 1960688 1962406 1960688 ID=MS.gene000144.t1;Parent=MS.gene000144
chr5.4 exon 1960688 1962406 1960688 ID=MS.gene000144.t1.exon1;Parent=MS.gene000144.t1
chr5.4 CDS 1960688 1962406 1960688 ID=cds.MS.gene000144.t1;Parent=MS.gene000144.t1
Gene Sequence

>MS.gene000144

ATGGCAGAACGAGAACCTGCATATACCGTTGCATCTGACAGTGAAACAACTGGGGAGGAAAAATCATCACCTCTTCCTGAAATAGCGATTGGAATTGATATTGGCACCTCACAATGTAGTGTTGCTGTGTGGAATGGCTCCGAAGTGGAGCTTTTGAAAAACAAAAGAAATCAGAAGCTTATGAAGTCATTTGTGACTTTCAAAGACGAAGCTCCTTCTGGAGGAGTTACCAGTCAATTCTCCCACGACCATGAGATGCTGTTTGGAGATACAATTTTCAATATGAAACGCTTGATCGGCAGAGTTGATACTGACCCTGTGGTTCACGCAAGTAAAAATCTGCCATTTCTGGTGCAGACCTTGGACATTGGCGTTCGTCCATTTATTGCTGCGTTAGTGAACAATGTCTGGAGATCCACCACTCCAGAAGAAGTCCTTGCAATGTTTCTGGTGGAGTTAAGACTGATGACTGAAACTCATCTCAAACGACCGATAAGAAATGTGGTGCTTACTGTTCCGGTTTCATTCAGTCGTTTCCAGCTGACCCGTTTAGAGCGTGCTTGTGCGATGGCTGGTCTTCATGTTCTCAGGTTGATGCCTGAACCAACAGCAGTGGCGTTATTATATGGACAACAACAACAGAAAGCTTCTCAAGAAACTATGGGCAGTGGAAGTGAGAAAATTGCTCTCATTTTCAATATGGGTGCTGGTTATTGTGATGTTGCTGTGACTGCTACCGCCGGGGGAGTATCCCAGATAAAAGCATTGGCAGGAAGTACCATCGGTGGAGAAGATTTGGTTCAGAATATGATGTGTTATCTCTTACCAGATTCTGAAAATATATTCAAGCGTCATGGGGTCAAAGAAATTAAATCAATGGCATTGCTTCGAGTTGCAACCCAGGATGCAATTCATCAGCTTTCCACCCAAAGCAGTGTTCAGGTTGATGTAGATTTGGGAGATGGTTTGAAGATATGCAAGGTTGTTGACAGGGCGGAGTTTGAGGAGGTGAACAATGAGGTGTTCGAAAAGTGTGAAAGCCTTATCATCCAATGTTTACATGATGCAAAGGTAGAAGTTGGGGATATAAATGATGTGATACTTGTTGGAGGATGTTCTTATATCCCAAGGGTGGAGAATCTTGTTACAACCTTATGCAAAATAACAGAAGTTTATAAAGGTATAAACCCTTTGGAAGGTGCGGTTTGTGGTGCAGCAATGGAAGGAGCTGTTGCTTCAGGCATCAGTGATCCCTTTGGAAACTTGGACTTGTTAACGATTCAAGCCACTCCTCTTGCCATTGGAATTCGAGCTGATGGGAACAAGTTTGTACCTGTAATTCCAAGGAACACCACAATGCCAGCACGTAAAGATCTACTTTTCACAACTATTCATGATAATCAAACTGAGGCATTGATACTTGTGTACGAAGGTGAGGGAAAGAAGGCAGAAGAAAACCACTTGTTAGGATATTTCAAGATAATGGGAATACCTGCAGCACCAAAAGGAGTTCCTGAAATCAGTGTGTGCATGGACATAGATGCTGCAAACGTGCTGCGAGTTTTAGCCGGTGTTGTGATGCCTGGATCCCGTATACCTGTGGTGCCTGTTATGGAAGTGAGGATGCCAATGGTTGATGATGGTCATGGTTGGTGTGCTGAGGCCCTAAATAGGACCTATGGTGCCACAATGGATTTGGTTACCCTCAAGAAGGCATAA
Protein sequence

>MS.gene000144.t1

MAEREPAYTVASDSETTGEEKSSPLPEIAIGIDIGTSQCSVAVWNGSEVELLKNKRNQKLMKSFVTFKDEAPSGGVTSQFSHDHEMLFGDTIFNMKRLIGRVDTDPVVHASKNLPFLVQTLDIGVRPFIAALVNNVWRSTTPEEVLAMFLVELRLMTETHLKRPIRNVVLTVPVSFSRFQLTRLERACAMAGLHVLRLMPEPTAVALLYGQQQQKASQETMGSGSEKIALIFNMGAGYCDVAVTATAGGVSQIKALAGSTIGGEDLVQNMMCYLLPDSENIFKRHGVKEIKSMALLRVATQDAIHQLSTQSSVQVDVDLGDGLKICKVVDRAEFEEVNNEVFEKCESLIIQCLHDAKVEVGDINDVILVGGCSYIPRVENLVTTLCKITEVYKGINPLEGAVCGAAMEGAVASGISDPFGNLDLLTIQATPLAIGIRADGNKFVPVIPRNTTMPARKDLLFTTIHDNQTEALILVYEGEGKKAEENHLLGYFKIMGIPAAPKGVPEISVCMDIDAANVLRVLAGVVMPGSRIPVVPVMEVRMPMVDDGHGWCAEALNRTYGATMDLVTLKKA