AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000228

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000228.t1 MTR_2g072850 96.784 342 6 2 1 338 66 406 0.0 691
MS.gene000228.t1 MTR_1g086170 60.177 339 130 3 2 338 145 480 1.39e-152 438
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000228.t1 AT1G17030 69.322 339 99 3 2 338 157 492 4.89e-174 493
MS.gene000228.t1 AT2G47010 63.743 342 119 3 2 342 154 491 5.51e-158 452
MS.gene000228.t1 AT2G47010 63.743 342 119 3 2 342 154 491 5.51e-158 452
MS.gene000228.t1 AT2G47010 65.132 304 101 3 2 304 154 453 5.47e-143 412
MS.gene000228.t1 AT4G09965 55.882 68 27 3 277 342 148 214 3.91e-16 77.0

Find 102 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCACGACGGTTCAGCTTC+TGG 0.163416 2.3:-23341991 None:intergenic
CTCCAAGTACATTGCATTTC+CGG 0.164623 2.3:-23342127 None:intergenic
GCTACTAATGATGCAAATTT+AGG 0.188556 2.3:+23341238 MS.gene000228:CDS
AAACCGGGTGCGGCAAAATT+TGG 0.201953 2.3:-23342203 None:intergenic
CTTCTTATTACTCAGTGATT+TGG 0.228669 2.3:+23341275 MS.gene000228:CDS
CATACACTTGTGTATAATTA+TGG 0.279040 2.3:-23341162 None:intergenic
ATGAGGTAGAATTGGCTCTT+TGG 0.289938 2.3:-23341877 None:intergenic
TATGTCCATTTCACTGTATC+TGG 0.292490 2.3:-23341965 None:intergenic
ACATCAAGCTCCCAAGTTCT+TGG 0.314890 2.3:-23342276 None:intergenic
TCCAAGTACATTGCATTTCC+GGG 0.320289 2.3:-23342126 None:intergenic
TGCACGACGGTTCAGCTTCT+GGG 0.328298 2.3:-23341990 None:intergenic
CAACCTTCACCTTTCTTTGT+TGG 0.329279 2.3:-23342243 None:intergenic
CACCCGGTTTGGGGCGAATA+CGG 0.332411 2.3:+23342216 MS.gene000228:CDS
TTCTTATTACTCAGTGATTT+GGG 0.333260 2.3:+23341276 MS.gene000228:CDS
TCAGTTCCCAAGTCAATTGA+TGG 0.350327 2.3:-23342530 None:intergenic
ATAGAGAAGGTAGGCATCAT+AGG 0.363316 2.3:-23342097 None:intergenic
TCATGCCTCTAGTTTCATAA+TGG 0.367780 2.3:-23341417 None:intergenic
CCATGTCCATGAGGTAGAAT+TGG 0.377010 2.3:-23341885 None:intergenic
ATCATGAGAGGAAGTTTCTA+TGG 0.378260 2.3:+23341199 MS.gene000228:CDS
GGAAAATCATGTTGTGGAAA+TGG 0.382470 2.3:-23341100 None:intergenic
ACCTTAAAATGTGGTGACTT+TGG 0.390926 2.3:-23341820 None:intergenic
AGGAAGTTGGTAATGGAAGT+TGG 0.407243 2.3:+23341302 MS.gene000228:CDS
TACCCGTATTCGCCCCAAAC+CGG 0.411510 2.3:-23342219 None:intergenic
TGAGGCTGCGGGTTGCTATA+AGG 0.416193 2.3:-23342171 None:intergenic
CTCTCCACAGGTTCAGTACC+TGG 0.420254 2.3:-23342500 None:intergenic
GGACATTGTTCAAGATGGTT+GGG 0.431398 2.3:-23342015 None:intergenic
GAGGAAGTTTCTATGGTTCA+TGG 0.446828 2.3:+23341206 MS.gene000228:CDS
TGGGAGAAGGAAGTTGGTAA+TGG 0.447993 2.3:+23341295 MS.gene000228:CDS
AGACTTCTTCTAAATATCCT+TGG 0.456198 2.3:+23341344 MS.gene000228:CDS
AGGACATTGTTCAAGATGGT+TGG 0.459205 2.3:-23342016 None:intergenic
TAACTGTCCATTCTGCTAGT+TGG 0.463954 2.3:-23342571 None:intergenic
GGATTGGTGATCCAAGAACT+TGG 0.474749 2.3:+23342265 MS.gene000228:CDS
GTAGGCATCATAGGGGAACT+TGG 0.477337 2.3:-23342088 None:intergenic
CCTTATCATACCTTCTCAAA+TGG 0.478700 2.3:+23342039 MS.gene000228:CDS
ACCCGGTTTGGGGCGAATAC+GGG 0.480308 2.3:+23342217 MS.gene000228:CDS
CATAATTATACACAAGTGTA+TGG 0.480843 2.3:+23341163 MS.gene000228:CDS
GATGCTACTACAGGATTCTC+TGG 0.483171 2.3:+23341388 MS.gene000228:CDS
TCCCGGAAATGCAATGTACT+TGG 0.490463 2.3:+23342125 MS.gene000228:CDS
CTTAGATCAGATGCTACTAC+AGG 0.490538 2.3:+23341379 MS.gene000228:CDS
TACCTTCTCTATCGTGCTCC+CGG 0.491517 2.3:+23342108 MS.gene000228:CDS
TGTAACCAGATACAGTGAAA+TGG 0.494064 2.3:+23341960 MS.gene000228:CDS
CACTTGATATCAAGAAAGGA+GGG 0.497483 2.3:+23341851 MS.gene000228:CDS
TAGAGAAGGTAGGCATCATA+GGG 0.498127 2.3:-23342096 None:intergenic
TCATGGACATGGGAAGTTGT+TGG 0.502759 2.3:+23341896 MS.gene000228:CDS
GGAGTATCTCTTGAGGCTGC+GGG 0.502864 2.3:-23342182 None:intergenic
GTATAATTATGGTACATTGA+TGG 0.503057 2.3:-23341151 None:intergenic
TGGAGTATCTCTTGAGGCTG+CGG 0.503368 2.3:-23342183 None:intergenic
AGGAAGTTTCTATGGTTCAT+GGG 0.503598 2.3:+23341207 MS.gene000228:CDS
TTACTCAGTGATTTGGGAGA+AGG 0.506110 2.3:+23341282 MS.gene000228:CDS
GTGATTTGGGAGAAGGAAGT+TGG 0.507494 2.3:+23341289 MS.gene000228:CDS
ACAATGGAAAGCCGTGCGAC+GGG 0.508747 2.3:+23341923 MS.gene000228:CDS
ACATATTTAATGCAGGATCC+AGG 0.510318 2.3:+23342482 MS.gene000228:intron
AGAACTTGGGAGCTTGATGT+TGG 0.511845 2.3:+23342279 MS.gene000228:CDS
ACCCGTATTCGCCCCAAACC+GGG 0.512133 2.3:-23342218 None:intergenic
GGAACTGAAATATACATTAG+TGG 0.514785 2.3:+23342545 MS.gene000228:CDS
CCAATTCTACCTCATGGACA+TGG 0.515374 2.3:+23341885 MS.gene000228:CDS
GGAACTTGGCTGCATCGGTT+CGG 0.516012 2.3:-23342074 None:intergenic
TATTCGCCCCAAACCGGGTG+CGG 0.522348 2.3:-23342213 None:intergenic
CAACAAAGAAAGGTGAAGGT+TGG 0.528934 2.3:+23342244 MS.gene000228:CDS
TTCACACTTGATATCAAGAA+AGG 0.531105 2.3:+23341847 MS.gene000228:CDS
TAAGGATCAGATGGTATCAC+TGG 0.533443 2.3:-23342153 None:intergenic
AAGGAGGACATTGTTCAAGA+TGG 0.534215 2.3:-23342020 None:intergenic
TGCATCGGTTCGGTGAACTC+TGG 0.535534 2.3:-23342064 None:intergenic
ATAGGGGAACTTGGCTGCAT+CGG 0.535545 2.3:-23342079 None:intergenic
CCATTTGAGAAGGTATGATA+AGG 0.536448 2.3:-23342039 None:intergenic
TACGGGTATCCAACAAAGAA+AGG 0.538937 2.3:+23342234 MS.gene000228:CDS
ACACTGGCCATCAATTGACT+TGG 0.543082 2.3:+23342523 MS.gene000228:CDS
AAGAAAGGTGAAGGTTGGAT+TGG 0.543649 2.3:+23342249 MS.gene000228:CDS
AAAGAGCCAATTCTACCTCA+TGG 0.544588 2.3:+23341879 MS.gene000228:CDS
GATGGCCAGTGTCTCTCCAC+AGG 0.551826 2.3:-23342512 None:intergenic
AACAATGGAAAGCCGTGCGA+CGG 0.555621 2.3:+23341922 MS.gene000228:CDS
CTGAACCTGTGGAGAGACAC+TGG 0.557518 2.3:+23342507 MS.gene000228:CDS
AACTCTGGTGCCATTTGAGA+AGG 0.573760 2.3:-23342049 None:intergenic
GATTGGTGATCCAAGAACTT+GGG 0.585095 2.3:+23342266 MS.gene000228:CDS
CACTGGCCATCAATTGACTT+GGG 0.586693 2.3:+23342524 MS.gene000228:CDS
GTTGAAAAGAAGATCATGAG+AGG 0.590017 2.3:+23341187 MS.gene000228:CDS
GTGGTGGCCAACTAGCAGAA+TGG 0.594829 2.3:+23342564 MS.gene000228:CDS
GGAAGTTGTTGGAAGAACAA+TGG 0.597086 2.3:+23341907 MS.gene000228:CDS
AGATGGTTGGGTTTGCACGA+CGG 0.607042 2.3:-23342003 None:intergenic
AAGCAAGGAAAATCATGTTG+TGG 0.610626 2.3:-23341106 None:intergenic
TTTGAGAAGGTATGATAAGG+AGG 0.612656 2.3:-23342036 None:intergenic
TATCCAACAAAGAAAGGTGA+AGG 0.620362 2.3:+23342240 MS.gene000228:CDS
ACACTTGATATCAAGAAAGG+AGG 0.620950 2.3:+23341850 MS.gene000228:CDS
TTCCGGGAGCACGATAGAGA+AGG 0.622485 2.3:-23342110 None:intergenic
CTTGATATCAAGAAAGGAGG+GGG 0.623013 2.3:+23341853 MS.gene000228:CDS
CAATTCTACCTCATGGACAT+GGG 0.628930 2.3:+23341886 MS.gene000228:CDS
AAAATTTGGAGTATCTCTTG+AGG 0.629876 2.3:-23342189 None:intergenic
TAGAAGAAGTCTTCAACACA+TGG 0.632132 2.3:-23341333 None:intergenic
GGATCCAGGTACTGAACCTG+TGG 0.632510 2.3:+23342496 MS.gene000228:intron
CAATGGAAAGCCGTGCGACG+GGG 0.635908 2.3:+23341924 MS.gene000228:CDS
GGGTTGCTATAAGGATCAGA+TGG 0.650869 2.3:-23342162 None:intergenic
ACTTGATATCAAGAAAGGAG+GGG 0.653164 2.3:+23341852 MS.gene000228:CDS
GATGTGACATCCCCGTCGCA+CGG 0.655142 2.3:-23341934 None:intergenic
CTAAGATAAAGCATTAACCA+AGG 0.661382 2.3:-23341361 None:intergenic
ACTGAAATATACATTAGTGG+TGG 0.661752 2.3:+23342548 MS.gene000228:CDS
ATACACCCTTCACCAAAGCA+AGG 0.670915 2.3:-23341121 None:intergenic
GGTTACCATTATGAAACTAG+AGG 0.672011 2.3:+23341412 MS.gene000228:CDS
GGGAGCACGATAGAGAAGGT+AGG 0.676074 2.3:-23342106 None:intergenic
AACAACTTCCCATGTCCATG+AGG 0.684995 2.3:-23341894 None:intergenic
GTGAACCTTACCTTAAAATG+TGG 0.685307 2.3:-23341829 None:intergenic
GCTACTACAGGATTCTCTGG+TGG 0.687235 2.3:+23341391 MS.gene000228:CDS
AGAGAAGGTAGGCATCATAG+GGG 0.755842 2.3:-23342095 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATATAAAATAAAATTATGT+AGG + chr2.3:23341795-23341814 MS.gene000228:intron 10.0%
!!! CATAATTTTATTTTATATGA+TGG - chr2.3:23341794-23341813 None:intergenic 10.0%
!! TTATTAGTAAATACTCAAAT+TGG + chr2.3:23341488-23341507 MS.gene000228:intron 15.0%
!!! TCAATCTCTTTATTTTTATC+AGG + chr2.3:23342311-23342330 MS.gene000228:CDS 20.0%
!!! TCTAAATTGGTACAATTTTA+AGG - chr2.3:23341515-23341534 None:intergenic 20.0%
!!! TTCAATAAGCATAATCTTTT+GGG - chr2.3:23342616-23342635 None:intergenic 20.0%
!!! TTTCAATAAGCATAATCTTT+TGG - chr2.3:23342617-23342636 None:intergenic 20.0%
! ATCATTTCAAGAAAAAGTCA+AGG - chr2.3:23342351-23342370 None:intergenic 25.0%
! CATAATTATACACAAGTGTA+TGG + chr2.3:23341163-23341182 MS.gene000228:CDS 25.0%
! CATACACTTGTGTATAATTA+TGG - chr2.3:23341165-23341184 None:intergenic 25.0%
! GTATAATTATGGTACATTGA+TGG - chr2.3:23341154-23341173 None:intergenic 25.0%
! GTTAACATATTTAAACCAGA+TGG + chr2.3:23341664-23341683 MS.gene000228:intron 25.0%
! TGCATCATCAAAATATATCA+AGG + chr2.3:23341706-23341725 MS.gene000228:intron 25.0%
! TTCTTATTACTCAGTGATTT+GGG + chr2.3:23341276-23341295 MS.gene000228:CDS 25.0%
ACTGAAATATACATTAGTGG+TGG + chr2.3:23342548-23342567 MS.gene000228:CDS 30.0%
AGACTTCTTCTAAATATCCT+TGG + chr2.3:23341344-23341363 MS.gene000228:CDS 30.0%
CTTCTTATTACTCAGTGATT+TGG + chr2.3:23341275-23341294 MS.gene000228:CDS 30.0%
GATCATTCCAAAATTGATAG+TGG - chr2.3:23342400-23342419 None:intergenic 30.0%
GCTACTAATGATGCAAATTT+AGG + chr2.3:23341238-23341257 MS.gene000228:CDS 30.0%
GCTGTTAACATATTTAATGC+AGG + chr2.3:23342475-23342494 MS.gene000228:intron 30.0%
GGAACTGAAATATACATTAG+TGG + chr2.3:23342545-23342564 MS.gene000228:CDS 30.0%
TTCACACTTGATATCAAGAA+AGG + chr2.3:23341847-23341866 MS.gene000228:CDS 30.0%
! AAAATTTGGAGTATCTCTTG+AGG - chr2.3:23342192-23342211 None:intergenic 30.0%
! CTAAGATAAAGCATTAACCA+AGG - chr2.3:23341364-23341383 None:intergenic 30.0%
!!! TCAGCTTTGAAACCATTTTT+TGG - chr2.3:23341079-23341098 None:intergenic 30.0%
AAGCAAGGAAAATCATGTTG+TGG - chr2.3:23341109-23341128 None:intergenic 35.0%
AAGCTAAGTCAATAATGTCG+CGG + chr2.3:23341633-23341652 MS.gene000228:intron 35.0%
ACACTTGATATCAAGAAAGG+AGG + chr2.3:23341850-23341869 MS.gene000228:CDS 35.0%
ACATATTTAATGCAGGATCC+AGG + chr2.3:23342482-23342501 MS.gene000228:intron 35.0%
ACCTTAAAATGTGGTGACTT+TGG - chr2.3:23341823-23341842 None:intergenic 35.0%
ACTTGATATCAAGAAAGGAG+GGG + chr2.3:23341852-23341871 MS.gene000228:CDS 35.0%
AGGAAGTTTCTATGGTTCAT+GGG + chr2.3:23341207-23341226 MS.gene000228:CDS 35.0%
ATCATGAGAGGAAGTTTCTA+TGG + chr2.3:23341199-23341218 MS.gene000228:CDS 35.0%
CACTTGATATCAAGAAAGGA+GGG + chr2.3:23341851-23341870 MS.gene000228:CDS 35.0%
CCTTATCATACCTTCTCAAA+TGG + chr2.3:23342039-23342058 MS.gene000228:CDS 35.0%
CTTAGCTTCAAATTCATCGA+TGG - chr2.3:23341621-23341640 None:intergenic 35.0%
GCTGCAATTTGTCCAAAAAA+TGG + chr2.3:23341064-23341083 MS.gene000228:CDS 35.0%
GGAAAATCATGTTGTGGAAA+TGG - chr2.3:23341103-23341122 None:intergenic 35.0%
GGTTACCATTATGAAACTAG+AGG + chr2.3:23341412-23341431 MS.gene000228:CDS 35.0%
GTGAACCTTACCTTAAAATG+TGG - chr2.3:23341832-23341851 None:intergenic 35.0%
GTTAATCAAGTTCTTCCATC+TGG - chr2.3:23341682-23341701 None:intergenic 35.0%
GTTGAAAAGAAGATCATGAG+AGG + chr2.3:23341187-23341206 MS.gene000228:CDS 35.0%
TAGAAGAAGTCTTCAACACA+TGG - chr2.3:23341336-23341355 None:intergenic 35.0%
TATCCAACAAAGAAAGGTGA+AGG + chr2.3:23342240-23342259 MS.gene000228:CDS 35.0%
TATGTCCATTTCACTGTATC+TGG - chr2.3:23341968-23341987 None:intergenic 35.0%
TCTAGTCCCTAAAATACCAT+CGG - chr2.3:23341580-23341599 None:intergenic 35.0%
TGTAACCAGATACAGTGAAA+TGG + chr2.3:23341960-23341979 MS.gene000228:CDS 35.0%
! ACCAAAGTCACCACATTTTA+AGG + chr2.3:23341819-23341838 MS.gene000228:CDS 35.0%
! ATGAGCTCCACTATCAATTT+TGG + chr2.3:23342390-23342409 MS.gene000228:intron 35.0%
! ATTTTCCTTGCTTTGGTGAA+GGG + chr2.3:23341116-23341135 MS.gene000228:CDS 35.0%
! CAACATGATTTTCCTTGCTT+TGG + chr2.3:23341109-23341128 MS.gene000228:CDS 35.0%
! CCATTTGAGAAGGTATGATA+AGG - chr2.3:23342042-23342061 None:intergenic 35.0%
! TCATGCCTCTAGTTTCATAA+TGG - chr2.3:23341420-23341439 None:intergenic 35.0%
! TTTGAGAAGGTATGATAAGG+AGG - chr2.3:23342039-23342058 None:intergenic 35.0%
!!! AGTCACCACATTTTAAGGTA+AGG + chr2.3:23341824-23341843 MS.gene000228:CDS 35.0%
AAAGAGCCAATTCTACCTCA+TGG + chr2.3:23341879-23341898 MS.gene000228:CDS 40.0%
AAGAAAGGTGAAGGTTGGAT+TGG + chr2.3:23342249-23342268 MS.gene000228:CDS 40.0%
AAGGAGGACATTGTTCAAGA+TGG - chr2.3:23342023-23342042 None:intergenic 40.0%
AGGACATTGTTCAAGATGGT+TGG - chr2.3:23342019-23342038 None:intergenic 40.0%
ATAGAGAAGGTAGGCATCAT+AGG - chr2.3:23342100-23342119 None:intergenic 40.0%
CAACAAAGAAAGGTGAAGGT+TGG + chr2.3:23342244-23342263 MS.gene000228:CDS 40.0%
CAACCTTCACCTTTCTTTGT+TGG - chr2.3:23342246-23342265 None:intergenic 40.0%
CAATTCTACCTCATGGACAT+GGG + chr2.3:23341886-23341905 MS.gene000228:CDS 40.0%
CTCCAAGTACATTGCATTTC+CGG - chr2.3:23342130-23342149 None:intergenic 40.0%
CTTAGATCAGATGCTACTAC+AGG + chr2.3:23341379-23341398 MS.gene000228:CDS 40.0%
CTTGATATCAAGAAAGGAGG+GGG + chr2.3:23341853-23341872 MS.gene000228:CDS 40.0%
GAGGAAGTTTCTATGGTTCA+TGG + chr2.3:23341206-23341225 MS.gene000228:CDS 40.0%
GATAACGTGCGAGTCTAAAT+TGG - chr2.3:23341528-23341547 None:intergenic 40.0%
GGAAGTTGTTGGAAGAACAA+TGG + chr2.3:23341907-23341926 MS.gene000228:CDS 40.0%
GGACATTGTTCAAGATGGTT+GGG - chr2.3:23342018-23342037 None:intergenic 40.0%
TAACTGTCCATTCTGCTAGT+TGG - chr2.3:23342574-23342593 None:intergenic 40.0%
TAAGGATCAGATGGTATCAC+TGG - chr2.3:23342156-23342175 None:intergenic 40.0%
TACGGGTATCCAACAAAGAA+AGG + chr2.3:23342234-23342253 MS.gene000228:CDS 40.0%
TAGAGAAGGTAGGCATCATA+GGG - chr2.3:23342099-23342118 None:intergenic 40.0%
TCAGTTCCCAAGTCAATTGA+TGG - chr2.3:23342533-23342552 None:intergenic 40.0%
TCCAAGTACATTGCATTTCC+GGG - chr2.3:23342129-23342148 None:intergenic 40.0%
! ATGAGGTAGAATTGGCTCTT+TGG - chr2.3:23341880-23341899 None:intergenic 40.0%
! GATTGGTGATCCAAGAACTT+GGG + chr2.3:23342266-23342285 MS.gene000228:CDS 40.0%
! GATTTTCCTTGCTTTGGTGA+AGG + chr2.3:23341115-23341134 MS.gene000228:CDS 40.0%
! GTCCATCCGATGGTATTTTA+GGG + chr2.3:23341571-23341590 MS.gene000228:intron 40.0%
! TGTCCATCCGATGGTATTTT+AGG + chr2.3:23341570-23341589 MS.gene000228:intron 40.0%
! TTACTCAGTGATTTGGGAGA+AGG + chr2.3:23341282-23341301 MS.gene000228:CDS 40.0%
!! AGGAAGTTGGTAATGGAAGT+TGG + chr2.3:23341302-23341321 MS.gene000228:CDS 40.0%
AACAACTTCCCATGTCCATG+AGG - chr2.3:23341897-23341916 None:intergenic 45.0%
ACACTGGCCATCAATTGACT+TGG + chr2.3:23342523-23342542 MS.gene000228:CDS 45.0%
ACATCAAGCTCCCAAGTTCT+TGG - chr2.3:23342279-23342298 None:intergenic 45.0%
AGAACTTGGGAGCTTGATGT+TGG + chr2.3:23342279-23342298 MS.gene000228:CDS 45.0%
AGAGAAGGTAGGCATCATAG+GGG - chr2.3:23342098-23342117 None:intergenic 45.0%
ATACACCCTTCACCAAAGCA+AGG - chr2.3:23341124-23341143 None:intergenic 45.0%
CCAATTCTACCTCATGGACA+TGG + chr2.3:23341885-23341904 MS.gene000228:CDS 45.0%
CCATGTCCATGAGGTAGAAT+TGG - chr2.3:23341888-23341907 None:intergenic 45.0%
GATCAATTGTGTCCATCCGA+TGG + chr2.3:23341561-23341580 MS.gene000228:intron 45.0%
GATGCTACTACAGGATTCTC+TGG + chr2.3:23341388-23341407 MS.gene000228:CDS 45.0%
GGGTTGCTATAAGGATCAGA+TGG - chr2.3:23342165-23342184 None:intergenic 45.0%
GTCCCTAAAATACCATCGGA+TGG - chr2.3:23341576-23341595 None:intergenic 45.0%
TCATGGACATGGGAAGTTGT+TGG + chr2.3:23341896-23341915 MS.gene000228:CDS 45.0%
TCCCGGAAATGCAATGTACT+TGG + chr2.3:23342125-23342144 MS.gene000228:CDS 45.0%
! CACTGGCCATCAATTGACTT+GGG + chr2.3:23342524-23342543 MS.gene000228:CDS 45.0%
! GGATTGGTGATCCAAGAACT+TGG + chr2.3:23342265-23342284 MS.gene000228:CDS 45.0%
! GTGATTTGGGAGAAGGAAGT+TGG + chr2.3:23341289-23341308 MS.gene000228:CDS 45.0%
!! AACTCTGGTGCCATTTGAGA+AGG - chr2.3:23342052-23342071 None:intergenic 45.0%
!! TGGGAGAAGGAAGTTGGTAA+TGG + chr2.3:23341295-23341314 MS.gene000228:CDS 45.0%
AAACCGGGTGCGGCAAAATT+TGG - chr2.3:23342206-23342225 None:intergenic 50.0%
AACAATGGAAAGCCGTGCGA+CGG + chr2.3:23341922-23341941 MS.gene000228:CDS 50.0%
AGATGGTTGGGTTTGCACGA+CGG - chr2.3:23342006-23342025 None:intergenic 50.0%
ATAGGGGAACTTGGCTGCAT+CGG - chr2.3:23342082-23342101 None:intergenic 50.0%
GCTACTACAGGATTCTCTGG+TGG + chr2.3:23341391-23341410 MS.gene000228:CDS 50.0%
GTAGGCATCATAGGGGAACT+TGG - chr2.3:23342091-23342110 None:intergenic 50.0%
TACCTTCTCTATCGTGCTCC+CGG + chr2.3:23342108-23342127 MS.gene000228:CDS 50.0%
! AAATTTTGCCGCACCCGGTT+TGG + chr2.3:23342205-23342224 MS.gene000228:CDS 50.0%
! AATTTTGCCGCACCCGGTTT+GGG + chr2.3:23342206-23342225 MS.gene000228:CDS 50.0%
! ACTCCAAATTTTGCCGCACC+CGG + chr2.3:23342200-23342219 MS.gene000228:CDS 50.0%
! TGGAGTATCTCTTGAGGCTG+CGG - chr2.3:23342186-23342205 None:intergenic 50.0%
ACAATGGAAAGCCGTGCGAC+GGG + chr2.3:23341923-23341942 MS.gene000228:CDS 55.0%
CTCTCCACAGGTTCAGTACC+TGG - chr2.3:23342503-23342522 None:intergenic 55.0%
CTGAACCTGTGGAGAGACAC+TGG + chr2.3:23342507-23342526 MS.gene000228:CDS 55.0%
GTGGTGGCCAACTAGCAGAA+TGG + chr2.3:23342564-23342583 MS.gene000228:CDS 55.0%
TACCCGTATTCGCCCCAAAC+CGG - chr2.3:23342222-23342241 None:intergenic 55.0%
TGCACGACGGTTCAGCTTCT+GGG - chr2.3:23341993-23342012 None:intergenic 55.0%
TTGCACGACGGTTCAGCTTC+TGG - chr2.3:23341994-23342013 None:intergenic 55.0%
! GGAGTATCTCTTGAGGCTGC+GGG - chr2.3:23342185-23342204 None:intergenic 55.0%
! GGGAGCACGATAGAGAAGGT+AGG - chr2.3:23342109-23342128 None:intergenic 55.0%
! TGAGGCTGCGGGTTGCTATA+AGG - chr2.3:23342174-23342193 None:intergenic 55.0%
! TTCCGGGAGCACGATAGAGA+AGG - chr2.3:23342113-23342132 None:intergenic 55.0%
!! ATTTTGCCGCACCCGGTTTG+GGG + chr2.3:23342207-23342226 MS.gene000228:CDS 55.0%
!! GGAACTTGGCTGCATCGGTT+CGG - chr2.3:23342077-23342096 None:intergenic 55.0%
!! GGATCCAGGTACTGAACCTG+TGG + chr2.3:23342496-23342515 MS.gene000228:intron 55.0%
!! TGCATCGGTTCGGTGAACTC+TGG - chr2.3:23342067-23342086 None:intergenic 55.0%
ACCCGTATTCGCCCCAAACC+GGG - chr2.3:23342221-23342240 None:intergenic 60.0%
CAATGGAAAGCCGTGCGACG+GGG + chr2.3:23341924-23341943 MS.gene000228:CDS 60.0%
GATGGCCAGTGTCTCTCCAC+AGG - chr2.3:23342515-23342534 None:intergenic 60.0%
GATGTGACATCCCCGTCGCA+CGG - chr2.3:23341937-23341956 None:intergenic 60.0%
TATTCGCCCCAAACCGGGTG+CGG - chr2.3:23342216-23342235 None:intergenic 60.0%
!! ACCCGGTTTGGGGCGAATAC+GGG + chr2.3:23342217-23342236 MS.gene000228:CDS 60.0%
!! CACCCGGTTTGGGGCGAATA+CGG + chr2.3:23342216-23342235 MS.gene000228:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 23341052 23342688 23341052 ID=MS.gene000228
chr2.3 mRNA 23341052 23342688 23341052 ID=MS.gene000228.t1;Parent=MS.gene000228
chr2.3 exon 23341052 23341447 23341052 ID=MS.gene000228.t1.exon1;Parent=MS.gene000228.t1
chr2.3 CDS 23341052 23341447 23341052 ID=cds.MS.gene000228.t1;Parent=MS.gene000228.t1
chr2.3 exon 23341817 23342332 23341817 ID=MS.gene000228.t1.exon2;Parent=MS.gene000228.t1
chr2.3 CDS 23341817 23342332 23341817 ID=cds.MS.gene000228.t1;Parent=MS.gene000228.t1
chr2.3 exon 23342497 23342589 23342497 ID=MS.gene000228.t1.exon3;Parent=MS.gene000228.t1
chr2.3 CDS 23342497 23342589 23342497 ID=cds.MS.gene000228.t1;Parent=MS.gene000228.t1
chr2.3 exon 23342665 23342688 23342665 ID=MS.gene000228.t1.exon4;Parent=MS.gene000228.t1
chr2.3 CDS 23342665 23342688 23342665 ID=cds.MS.gene000228.t1;Parent=MS.gene000228.t1
Gene Sequence

>MS.gene000228

ATGGATACTTTAGCTGCAATTTGTCCAAAAAATGGTTTCAAAGCTGAGCCATTTCCACAACATGATTTTCCTTGCTTTGGTGAAGGGTGTATGAATATGCCATCAATGTACCATAATTATACACAAGTGTATGGTGTTGAAAAGAAGATCATGAGAGGAAGTTTCTATGGTTCATGGGATTTGAATGCTACTAATGATGCAAATTTAGGTTATATGAATGATACTTCTTATTACTCAGTGATTTGGGAGAAGGAAGTTGGTAATGGAAGTTGGATTTTTCACCATGTGTTGAAGACTTCTTCTAAATATCCTTGGTTAATGCTTTATCTTAGATCAGATGCTACTACAGGATTCTCTGGTGGTTACCATTATGAAACTAGAGGCATGACAAAAATTGTGAGTTTTTTTCTTTCTACTTTTAAGTTTATTAGCATTTTTATTAGTAAATACTCAAATTGGTCCTTAAAATTGTACCAATTTAGACTCGCACGTTATCGATATTCTATAATGATCAATTGTGTCCATCCGATGGTATTTTAGGGACTAGAATGTCATTAATTGAAATACCATCGATGAATTTGAAGCTAAGTCAATAATGTCGCGGATGCATTTGTTAACATATTTAAACCAGATGGAAGAACTTGATTAACATTTTGCATCATCAAAATATATCAAGGTTCATTTTGAAATGTTGATAAGTTGAATATTTACTATTTTCTTCTTATGCTTATGAAGTCATCCATCATATAAAATAAAATTATGTAGGTACCAAAGTCACCACATTTTAAGGTAAGGTTCACACTTGATATCAAGAAAGGAGGGGGTCCAAAGAGCCAATTCTACCTCATGGACATGGGAAGTTGTTGGAAGAACAATGGAAAGCCGTGCGACGGGGATGTCACATCTGATGTAACCAGATACAGTGAAATGGACATAAACCCAGAAGCTGAACCGTCGTGCAAACCCAACCATCTTGAACAATGTCCTCCTTATCATACCTTCTCAAATGGCACCAGAGTTCACCGAACCGATGCAGCCAAGTTCCCCTATGATGCCTACCTTCTCTATCGTGCTCCCGGAAATGCAATGTACTTGGAGAAACCAGTGATACCATCTGATCCTTATAGCAACCCGCAGCCTCAAGAGATACTCCAAATTTTGCCGCACCCGGTTTGGGGCGAATACGGGTATCCAACAAAGAAAGGTGAAGGTTGGATTGGTGATCCAAGAACTTGGGAGCTTGATGTTGGAAGACTCTCTCAATCTCTTTATTTTTATCAGGCAAGTTCTCATGTTCCTTGACTTTTTCTTGAAATGATGATTCAAACAACTTATATTATGAGCTCCACTATCAATTTTGGAATGATCATTGATGAAAGTAGAAGAAATTAGAAATACTTCGTATTTTATATTAACAATATTTGCTGTTAACATATTTAATGCAGGATCCAGGTACTGAACCTGTGGAGAGACACTGGCCATCAATTGACTTGGGAACTGAAATATACATTAGTGGTGGCCAACTAGCAGAATGGACAGTTAGCAACTTTGATATTATCATCCCAAAAGATTATGCTTATTGAAATTTCAAGATTAACAATTTATATGTTTAGCTCAGCAACACTGCAGCTTTGTAG
Protein sequence

>MS.gene000228.t1

MDTLAAICPKNGFKAEPFPQHDFPCFGEGCMNMPSMYHNYTQVYGVEKKIMRGSFYGSWDLNATNDANLGYMNDTSYYSVIWEKEVGNGSWIFHHVLKTSSKYPWLMLYLRSDATTGFSGGYHYETRGMTKIVPKSPHFKVRFTLDIKKGGGPKSQFYLMDMGSCWKNNGKPCDGDVTSDVTRYSEMDINPEAEPSCKPNHLEQCPPYHTFSNGTRVHRTDAAKFPYDAYLLYRAPGNAMYLEKPVIPSDPYSNPQPQEILQILPHPVWGEYGYPTKKGEGWIGDPRTWELDVGRLSQSLYFYQDPGTEPVERHWPSIDLGTEIYISGGQLAEWTLSNTAAL